Carg06124 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg06124
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Peptidyl-tRNA hydrolase family protein
Location	Cucurbita_argyrosperma_scaffold_026 : 1189894 .. 1193131 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGTATCTCTCTCTTTCATTCTCTTCACTTCGTCTTTGATTTTTCAATTTTCCAGTTGAATTCAAGGTCTGGTACCACCGTCGGAACAGCTGCGATCGGCGATAACAATCGGCGGCGGGGGCGAAATGAAACAACCGCTAGTGGCTGCCGCATCGCACTGTTCGTCCTTTTTCTTCTTTTTTGCTGTTAATCCTGACATTGTATTTGTAATCTGCGTTAGAATCAACTCAGGGACATGACTCGAGTGCGGAATTGAATTGCCAATAAATTTTTGTTTATTACATTTACATTTTGGGGTTGTTTCTTTTGTTCTTAATTGGTTAGGAGCTGATCGAATTTTTTGGTTTTCAGTCAATAGGGAAGTGTATGAGGGTTTGTTTTAGTGGAAAATGTTCAATAAGTTGTCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAATTCAGAGGAACCAGGCACAATGTAATTACTGCATTTTGATTTTGAAATGAGAGATATTAGTTCGTTCATTCTTATTTTGGTTCAGTACAGGTGGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGTGAAGGCATGTTCACTTTTTTGTTTACAGGACTTGTTGTTTTTCTTTTGATTGCATGAAGTTGAAGGATGTTTGTGTATTGATATGTCAGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAGACTTACATGAACTTGAGTGGTGAATCTGTATGTGATCGAGAAACTCCTTTTGGCTTCTGTCTTGTTATAATTTAGGTTCCGTTTAGTCAATGTTTTTTTATTGCTCGAGTTCACGAATTTATTGGAGTGAATTCATCTTTCAACTCGATGACTTCAAGGAAAAAAAAACACTCCGTATTCGATTTAGGATCTATTATGAGCGGTTCTTGTGATGGTTGTCATAGCTAGTTCCTTACTGAGATTGGTTTGATGCCATAAATCTTGTGTTTTGTCATCATATTGCAGGCAGGACCGCTTGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGGTAAATTCTTATGTGAATTTGAATCCTTTTCTAATTACATAGTTCTTAAATGATCAAAGGCATGGTATTATTTCGTGTACTTAACCTTTGCAGTTCCATGATGACATGAACTTGCCATGTGGGGTGCTTCGGCTTCGACATAATGGAGGGAATGACTCCCACAACGGGTATCGTTGATCTCTTTTTTTTTCTCCTTATCCTCTTCTGTTGTAGCCTATTGCCACTCTATAGCCACAAGCTAGGTTGCCCCATGTTTCCACACATGTTTGTGACATTGACATACCTTGACTGAATTCCATTAGTTGTTTTCATTTGAAAAGAAAAAGTGTGAGATCCCACATTGATTGGAGAGGGGAACGAAGCATTCCTTATAAAGGTGTGGAAACCTCTCCATAACATACATATTTTAAAACTTTGAAGGGAAGCCCAAAAGGGAAAGCTAAAAGAGTATAGTATTTGCTAGCGGTGGGCTTGGGCTATTACAAATGGTATCAGAGCTAGACACCGAGCGGTGTGCCAACAAGGACGCTGGCCCCCCAAGGGGAGTGGATTGTGAGATCCACATCGGTTCGAGAGGGGAACGAAGTATTTCTTATTAGGGTGTGGAAACTTCTCCTTAACAAACGTGTTTTAAAAACCTTGAGGGGAAGCCCAGAAAGGAAAGCCAAAAGAGGACAATATCTACTAGTAGTGAGCTTGGACTCTTACAAAAAAGAGTAACAATAAGAGTATGTAAGATGGTTTATTATATCAACATTTGATTTAGCGTCCAGCATGCTAGAGATATGATGATAATTACATCCTATTTGGACTGCTTTTTTATGTTTGGTCATGTTAATTAATCGTTCCATTATCCGTACGCGCTTGAAGAATCGAACAAAGCATTTGTGGGGTCAAAGGTATCATACATAAAGTCCTAATACAACTTTCAGAATGTTAGCTATTGCCATGAAGTTTTATCAACGTGATTCCTCTAAACATCATGTCTAGCTCTCAAGCTACAGCTTGAATTCTTTTTTTGTAACGACATACTACTAATCGTCCGTGCTTATTCTTTCTTTAGTAAAACCTTCACGACTTCTCGTCCATTCTATCGCCTTTTCTGGTACATCTTGAAATGGATTTTTATTTTAGGCTGAAGAGTGTCATTTATCACTTTCGAGGGAACCGAGAGTTTGCTAGGTTAAGAATTGGTAAGTATTGTTGCCTATGCTACTTTGTGGTCTTTTACGTGTTTCTCCTCCGTTCTTAGAACGTTTTCTTTATAATAGGCATTGGAAGGCCACCTGGTCAAATGGATCCCAAAGCATTCTTGCTTCAAAAGTTCAATAACACAGCTCAAGAACGAGTGAGTCTTTTGCTGATTATACTACACGAAACTCATGGTTGAAACCTTTCTATCTTTCTTTGAGACCCCATGAAATTTCATCTGCAAATCACTGCTTACCTTATATTCACTCTACATTCTTTTGCTTGAAAAATATATTTTGGACCCTCTTGAGTCCCACTTTTAATTTTCGGCATGTATAGTTTGCGTCTTATAATCCTCGATTACATTATGATTATTACTCATCTCGGCATAACGCTTCCCTTATTTTTTTGTTTAAAAAGATTGATACTGCATTGCAAGAGGGGGTTGGTGCATTGAAGCTCCTATTGTCTCTCGGTTTATCCGAGACTGCGAGACGCTTCAACCACGAACAGAAATACAAGCATATAAGGCTACAGACCATGCCAACATGATGAGCATACTCTCTGCTTGTTTCCATTGCCAATGGAACATACAAATTCTACCAAGTACTCAAAGGGAAGGGGAGCACAGAGGCCGAGTGGATGTCCTCCGAAGGTACAATGACTTTGTGTTCTAAGATATTGGGGCCAGCGTGTGGAGAATTTAGAGGACGTTTTGAGATATCCTGTCTCTATTATGTAATCATTTTTTGCTTACAACTGATAAACTTTATAAGCTTAGGTACTTGAAAAATTGCCTCATGATTAATTTATATTTATAGAAGTGAAGCCGATTGTTTTTGTATTTTTTTTTTCTCGTTCGAGTTCATAAGCTCTCTTAAAGAGAATTAATTTAGACGTTTTTGGTCAATTTATATTAATCGTTACATT

mRNA sequence

AAGGTATCTCTCTCTTTCATTCTCTTCACTTCGTCTTTGATTTTTCAATTTTCCAGTTGAATTCAAGGTCTGGTACCACCGTCGGAACAGCTGCGATCGGCGATAACAATCGGCGGCGGGGGCGAAATGAAACAACCGCTAGTGGCTGCCGCATCGCACTTCAATAGGGAAGTGTATGAGGGTTTGTTTTAGTGGAAAATGTTCAATAAGTTGTCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAATTCAGAGGAACCAGGCACAATGTGGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGATGTTTGTGTATTGATATGTCAGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAGACTTACATGAACTTGAGTGGTGAATCTGCAGGACCGCTTGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGTTCCATGATGACATGAACTTGCCATGTGGGGTGCTTCGGCTTCGACATAATGGAGGGAATGACTCCCACAACGGGCTGAAGAGTGTCATTTATCACTTTCGAGGGAACCGAGAGTTTGCTAGGTTAAGAATTGGCATTGGAAGGCCACCTGGTCAAATGGATCCCAAAGCATTCTTGCTTCAAAAGTTCAATAACACAGCTCAAGAACGAATTGATACTGCATTGCAAGAGGGGGTTGGTGCATTGAAGCTCCTATTGTCTCTCGGTTTATCCGAGACTGCGAGACGCTTCAACCACGAACAGAAATACAAGCATATAAGGCTACAGACCATGCCAACATGATGAGCATACTCTCTGCTTGTTTCCATTGCCAATGGAACATACAAATTCTACCAAGTACTCAAAGGGAAGGGGAGCACAGAGGCCGAGTGGATGTCCTCCGAAGGTACAATGACTTTGTGTTCTAAGATATTGGGGCCAGCGTGTGGAGAATTTAGAGGACGTTTTGAGATATCCTGTCTCTATTATGTAATCATTTTTTGCTTACAACTGATAAACTTTATAAGCTTAGGTACTTGAAAAATTGCCTCATGATTAATTTATATTTATAGAAGTGAAGCCGATTGTTTTTGTATTTTTTTTTTCTCGTTCGAGTTCATAAGCTCTCTTAAAGAGAATTAATTTAGACGTTTTTGGTCAATTTATATTAATCGTTACATT

Coding sequence (CDS)

ATGTTCAATAAGTTGTCGAAGCGTGGCTTTTGCACGGCAATATCATCACGGCCTTGGCTTTTTGTGGGCTTGGGCAATCCTGGAGAGAAATTCAGAGGAACCAGGCACAATGTGGGGTTTGAGATGATTGATGTGTTTGCTGAGTCTGTTGGAATTCCAATGAACAGGATTCACTGCAAAGCTGTGTTTGGATGTTTGTGTATTGATATGTCAGGTTTTGTTGGGGACATCCCTGTTTTTCTGGCAAAGCCTCAGACTTACATGAACTTGAGTGGTGAATCTGCAGGACCGCTTGCAGCATATTACAAGCTACCTCTAAACCGTGTGCTCGTGTTCCATGATGACATGAACTTGCCATGTGGGGTGCTTCGGCTTCGACATAATGGAGGGAATGACTCCCACAACGGGCTGAAGAGTGTCATTTATCACTTTCGAGGGAACCGAGAGTTTGCTAGGTTAAGAATTGGCATTGGAAGGCCACCTGGTCAAATGGATCCCAAAGCATTCTTGCTTCAAAAGTTCAATAACACAGCTCAAGAACGAATTGATACTGCATTGCAAGAGGGGGTTGGTGCATTGAAGCTCCTATTGTCTCTCGGTTTATCCGAGACTGCGAGACGCTTCAACCACGAACAGAAATACAAGCATATAAGGCTACAGACCATGCCAACATGA

Protein sequence

MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT

Homology

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BLAST of Carg06124 vs. NCBI nr
Match: XP_022961338.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita moschata] >XP_022961339.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 449.5 bits (1155), Expect = 6.6e-123
Identity = 217/224 (96.88%), Postives = 217/224 (96.88%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           AVFG       GFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC
Sbjct: 61  AVFG------EGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE
Sbjct: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 225
           RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT
Sbjct: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 218

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BLAST of Carg06124 vs. NCBI nr
Match: XP_022988207.1 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita maxima] >XP_022988208.1 peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 447.2 bits (1149), Expect = 3.3e-122
Identity = 215/224 (95.98%), Postives = 216/224 (96.43%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           AVFG       GFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC
Sbjct: 61  AVFG------EGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE
Sbjct: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 225
           RIDTALQEG+GALKLLLSLGLSETAR FNHEQKYKHIRLQTMPT
Sbjct: 181 RIDTALQEGIGALKLLLSLGLSETARHFNHEQKYKHIRLQTMPT 218

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BLAST of Carg06124 vs. NCBI nr
Match: XP_023532821.1 (peptidyl-tRNA hydrolase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532822.1 peptidyl-tRNA hydrolase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 445.3 bits (1144), Expect = 1.2e-121
Identity = 215/224 (95.98%), Postives = 215/224 (95.98%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK
Sbjct: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           AVFG       GFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC
Sbjct: 61  AVFG------EGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRLRHNGGNDSHNGLKSVIYHFRGNREFAR RIGIGRPPGQMDPKAFLLQKFNNTAQE
Sbjct: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARFRIGIGRPPGQMDPKAFLLQKFNNTAQE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 225
           RIDTALQEGVGALKLLLS GLSETARRFNHEQKYKHIRLQTMPT
Sbjct: 181 RIDTALQEGVGALKLLLSHGLSETARRFNHEQKYKHIRLQTMPT 218

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BLAST of Carg06124 vs. NCBI nr
Match: XP_008451022.1 (PREDICTED: peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 427.2 bits (1097), Expect = 3.5e-116
Identity = 204/223 (91.48%), Postives = 211/223 (94.62%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           MFNKL+KRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGF+MIDVFAESVGIPMNR+HCK
Sbjct: 1   MFNKLTKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFQMIDVFAESVGIPMNRVHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           AVFG       GFVGDIPVFLAKPQTYMNLSGESAGPLAA+YKLPLNRVLVFHDDMNLPC
Sbjct: 61  AVFG------EGFVGDIPVFLAKPQTYMNLSGESAGPLAAHYKLPLNRVLVFHDDMNLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRL+HNGG+D HNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE
Sbjct: 121 GVLRLQHNGGHDYHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMP 224
           RIDTALQEGVGALK LLS GLSE+ R FNHEQKYKHIRLQTMP
Sbjct: 181 RIDTALQEGVGALKRLLSHGLSESVRHFNHEQKYKHIRLQTMP 217

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BLAST of Carg06124 vs. NCBI nr
Match: XP_022147698.1 (peptidyl-tRNA hydrolase, mitochondrial [Momordica charantia])

HSP 1 Score: 421.8 bits (1083), Expect = 1.5e-114
Identity = 202/224 (90.18%), Postives = 212/224 (94.64%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           MF+KL+KRGFCTA+S RPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIP+NR+HCK
Sbjct: 1   MFSKLTKRGFCTALSPRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPINRVHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           A+FG       GFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC
Sbjct: 61  ALFG------EGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRLR +GG+D HNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTA+E
Sbjct: 121 GVLRLRPDGGHDYHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTARE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 225
           RIDTAL EGVGALK LLSLGLSE+ARRFNHEQKYKHIRLQTMPT
Sbjct: 181 RIDTALLEGVGALKDLLSLGLSESARRFNHEQKYKHIRLQTMPT 218

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BLAST of Carg06124 vs. TAIR10
Match: AT5G19830.1 (Peptidyl-tRNA hydrolase family protein)

HSP 1 Score: 327.8 bits (839), Expect = 5.3e-90
Identity = 155/222 (69.82%), Postives = 183/222 (82.43%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           M ++LS+R +CT+   RPWLF+GLGNPG+K++GTRHN+GFEMIDVFAESVGI MN ++ K
Sbjct: 1   MLSRLSRRCYCTSSVHRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           A+ G       GFV D+PV LAKPQTYMNLSGES+GPLAAYYKLPLNRVLV HDDM LPC
Sbjct: 61  AIMG------QGFVADLPVILAKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRL+  GG+  HNGLKSV+ HFRGNREFARLRIGIG+PPGQMDPKAFLLQKF+  A+E
Sbjct: 121 GVLRLQEKGGHGCHNGLKSVMNHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTM 223
           R+D AL EGV ALKL+L+    E+ R FN EQKYKH++  T+
Sbjct: 181 RMDKALAEGVDALKLVLAKDFGESWRLFNVEQKYKHLKQHTI 216

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BLAST of Carg06124 vs. TAIR10
Match: AT5G16140.1 (Peptidyl-tRNA hydrolase family protein)

HSP 1 Score: 230.3 bits (586), Expect = 1.1e-60
Identity = 111/198 (56.06%), Postives = 139/198 (70.20%), Query Frame = 0


Query: 18  PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDI 77
           PWL VGLGNPG K+ GTRHNVGFEMIDV A   G+ MN I  KA+ G       G + D+
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGI------GAIEDV 104

Query: 78  PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGL 137
           P+ LAKPQTYMN SGES G LA++Y++PL  +L+ +D+M LP GVLRL+  GG   HNG+
Sbjct: 105 PILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGV 164

Query: 138 KSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLL 197
           KSV+ H  G R F RL IGIG+PPG MD KAFLLQKF+   Q++I+ AL++G  A+K L+
Sbjct: 165 KSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLV 224

Query: 198 SLGLSETARRFNHEQKYK 216
             G ++   RFN  QKYK
Sbjct: 225 LNGFNQGISRFNLVQKYK 236

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BLAST of Carg06124 vs. TAIR10
Match: AT5G38290.2 (Peptidyl-tRNA hydrolase family protein)

HSP 1 Score: 226.9 bits (577), Expect = 1.3e-59
Identity = 109/198 (55.05%), Postives = 136/198 (68.69%), Query Frame = 0


Query: 18  PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDI 77
           PWL VGLGNPG K+ GTRHN+GFEMID  A +  I MN I  KA+ G       G VG++
Sbjct: 55  PWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQSKALVGI------GSVGEV 114

Query: 78  PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGL 137
           P+ L KPQ YMN SGES GPLAAYY++PL  +L+ +DDM L  GVLRL+  GG+  HNGL
Sbjct: 115 PILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGL 174

Query: 138 KSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLL 197
           K+V  H  G R + RL IGIG PPG MD KAFLLQKF+   ++++D  L++GV  +K L+
Sbjct: 175 KNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLV 234

Query: 198 SLGLSETARRFNHEQKYK 216
             G S++  RFN  QKYK
Sbjct: 235 EEGFSDSISRFNLGQKYK 246

[Close]

BLAST of Carg06124 vs. TAIR10
Match: AT1G18440.1 (Peptidyl-tRNA hydrolase family protein)

HSP 1 Score: 226.5 bits (576), Expect = 1.7e-59
Identity = 102/200 (51.00%), Postives = 145/200 (72.50%), Query Frame = 0


Query: 18  PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDI 77
           PWL VGLGNPG+K++GTRHNVGFEM+D  A++ GI MN ++ KA+FG       G +G+I
Sbjct: 94  PWLIVGLGNPGKKYQGTRHNVGFEMVDALADAEGISMNTVNFKALFG------KGVIGNI 153

Query: 78  PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGL 137
           P+ LAKPQT+MNLSGES G + ++YK+PL +VLV +DD++LP G LRL   GG+  HNG+
Sbjct: 154 PIMLAKPQTFMNLSGESVGQIVSFYKIPLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNGM 213

Query: 138 KSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLL 197
           +S+I   +G+R+F RLRIGIGRPPG+MD   F+L++FN   QE +D   Q G+ A+++LL
Sbjct: 214 RSIIDRLKGSRDFPRLRIGIGRPPGKMDTANFVLRQFNRQEQEELDHTFQTGLEAIRILL 273

Query: 198 SLGLSETARRFNHEQKYKHI 218
             G +++A   N  +  + +
Sbjct: 274 LEGFNKSATFVNTRKSMEQL 287

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BLAST of Carg06124 vs. Swiss-Prot
Match: sp|Q6NLS8|PTHM_ARATH (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 9.5e-89
Identity = 155/222 (69.82%), Postives = 183/222 (82.43%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           M ++LS+R +CT+   RPWLF+GLGNPG+K++GTRHN+GFEMIDVFAESVGI MN ++ K
Sbjct: 1   MLSRLSRRCYCTSSVHRPWLFLGLGNPGDKYKGTRHNIGFEMIDVFAESVGIQMNLVNFK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           A+ G       GFV D+PV LAKPQTYMNLSGES+GPLAAYYKLPLNRVLV HDDM LPC
Sbjct: 61  AIMG------QGFVADLPVILAKPQTYMNLSGESSGPLAAYYKLPLNRVLVVHDDMQLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRL+  GG+  HNGLKSV+ HFRGNREFARLRIGIG+PPGQMDPKAFLLQKF+  A+E
Sbjct: 121 GVLRLQEKGGHGCHNGLKSVMNHFRGNREFARLRIGIGKPPGQMDPKAFLLQKFSMPARE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTM 223
           R+D AL EGV ALKL+L+    E+ R FN EQKYKH++  T+
Sbjct: 181 RMDKALAEGVDALKLVLAKDFGESWRLFNVEQKYKHLKQHTI 216

[Close]

BLAST of Carg06124 vs. Swiss-Prot
Match: sp|Q9M5P4|CRS2_MAIZE (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays OX=4577 GN=CRS2 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.3e-61
Identity = 112/202 (55.45%), Postives = 145/202 (71.78%), Query Frame = 0


Query: 18  PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDI 77
           PWL  GLGNPG K+ GTRHNVGFEM+D  A   GI MN I  K++ G       G +G++
Sbjct: 61  PWLIAGLGNPGNKYYGTRHNVGFEMVDRIAAEEGITMNTIQSKSLLGI------GSIGEV 120

Query: 78  PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGL 137
           PV + KPQ+YMN SGE+ GPLAAYY++PL  +L+ +DD +LP GVLRL+  GG+  HNGL
Sbjct: 121 PVLVVKPQSYMNYSGEAIGPLAAYYQVPLRHILLIYDDTSLPNGVLRLQKKGGHGRHNGL 180

Query: 138 KSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLL 197
           ++VI H  G REF RL IGIG PPG+MDP+AFLLQKF++  + +IDTAL++GV A++ L+
Sbjct: 181 QNVIEHLDGRREFPRLSIGIGSPPGKMDPRAFLLQKFSSEERVQIDTALEQGVDAVRTLV 240

Query: 198 SLGLSETARRFNHEQKYKHIRL 220
             G S +  RFN  QKYK  R+
Sbjct: 241 LKGFSGSTERFNLVQKYKFHRV 256

[Close]

BLAST of Carg06124 vs. Swiss-Prot
Match: sp|Q9LF14|CRS2B_ARATH (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2B PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.1e-59
Identity = 111/198 (56.06%), Postives = 139/198 (70.20%), Query Frame = 0


Query: 18  PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDI 77
           PWL VGLGNPG K+ GTRHNVGFEMIDV A   G+ MN I  KA+ G       G + D+
Sbjct: 45  PWLIVGLGNPGNKYHGTRHNVGFEMIDVLARKEGVLMNTIQSKALIGI------GAIEDV 104

Query: 78  PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGL 137
           P+ LAKPQTYMN SGES G LA++Y++PL  +L+ +D+M LP GVLRL+  GG   HNG+
Sbjct: 105 PILLAKPQTYMNFSGESVGSLASHYRVPLRHILMIYDEMALPNGVLRLQPKGGQGYHNGV 164

Query: 138 KSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLL 197
           KSV+ H  G R F RL IGIG+PPG MD KAFLLQKF+   Q++I+ AL++G  A+K L+
Sbjct: 165 KSVMGHLDGRRNFPRLSIGIGKPPGNMDMKAFLLQKFSPLEQKQIEEALEQGSEAVKTLV 224

Query: 198 SLGLSETARRFNHEQKYK 216
             G ++   RFN  QKYK
Sbjct: 225 LNGFNQGISRFNLVQKYK 236

[Close]

BLAST of Carg06124 vs. Swiss-Prot
Match: sp|Q9FKN4|CRS2A_ARATH (Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2A PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.3e-58
Identity = 109/198 (55.05%), Postives = 136/198 (68.69%), Query Frame = 0


Query: 18  PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDI 77
           PWL VGLGNPG K+ GTRHN+GFEMID  A +  I MN I  KA+ G       G VG++
Sbjct: 51  PWLIVGLGNPGLKYYGTRHNIGFEMIDHIARATDISMNTIQSKALVGI------GSVGEV 110

Query: 78  PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGL 137
           P+ L KPQ YMN SGES GPLAAYY++PL  +L+ +DDM L  GVLRL+  GG+  HNGL
Sbjct: 111 PILLVKPQGYMNFSGESVGPLAAYYQIPLRHILMIYDDMGLSNGVLRLQPKGGHSQHNGL 170

Query: 138 KSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLL 197
           K+V  H  G R + RL IGIG PPG MD KAFLLQKF+   ++++D  L++GV  +K L+
Sbjct: 171 KNVTEHLNGCRGYPRLSIGIGNPPGNMDMKAFLLQKFSPLERKQMDEGLEQGVEGVKTLV 230

Query: 198 SLGLSETARRFNHEQKYK 216
             G S++  RFN  QKYK
Sbjct: 231 EEGFSDSISRFNLGQKYK 242

[Close]

BLAST of Carg06124 vs. Swiss-Prot
Match: sp|Q5ZCL8|CRS2_ORYSJ (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0132800 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 3.0e-58
Identity = 107/198 (54.04%), Postives = 141/198 (71.21%), Query Frame = 0


Query: 18  PWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCKAVFGCLCIDMSGFVGDI 77
           PWL  GLGNPG K+ GTRHNVGFEM+D  A   GI MN I  K++ G       G +G++
Sbjct: 64  PWLIAGLGNPGSKYHGTRHNVGFEMVDRIARDEGITMNTIQSKSLLGI------GSIGEV 123

Query: 78  PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPCGVLRLRHNGGNDSHNGL 137
           PV L KPQ+Y+N SGE+ GPLAAYY++PL  +LV +D+M+LP GVLRL+  GG+  HNGL
Sbjct: 124 PVLLVKPQSYINYSGEAIGPLAAYYQVPLRHILVMYDEMSLPNGVLRLQRKGGHGRHNGL 183

Query: 138 KSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQERIDTALQEGVGALKLLL 197
           ++V+     +RE  RL IGIG PPG+MD +AFLLQKF++  + +IDTAL++GV A++ L+
Sbjct: 184 QNVMECLDSSRELPRLSIGIGSPPGKMDTRAFLLQKFSSEERLQIDTALEQGVDAVRTLV 243

Query: 198 SLGLSETARRFNHEQKYK 216
             G S +  RFN  QKYK
Sbjct: 244 LKGFSGSIERFNLVQKYK 255

[Close]

BLAST of Carg06124 vs. TrEMBL
Match: tr|A0A1S3BPZ7|A0A1S3BPZ7_CUCME (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492429 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 2.3e-116
Identity = 204/223 (91.48%), Postives = 211/223 (94.62%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           MFNKL+KRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGF+MIDVFAESVGIPMNR+HCK
Sbjct: 1   MFNKLTKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFQMIDVFAESVGIPMNRVHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           AVFG       GFVGDIPVFLAKPQTYMNLSGESAGPLAA+YKLPLNRVLVFHDDMNLPC
Sbjct: 61  AVFG------EGFVGDIPVFLAKPQTYMNLSGESAGPLAAHYKLPLNRVLVFHDDMNLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRL+HNGG+D HNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE
Sbjct: 121 GVLRLQHNGGHDYHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMP 224
           RIDTALQEGVGALK LLS GLSE+ R FNHEQKYKHIRLQTMP
Sbjct: 181 RIDTALQEGVGALKRLLSHGLSESVRHFNHEQKYKHIRLQTMP 217

[Close]

BLAST of Carg06124 vs. TrEMBL
Match: tr|A0A0A0LWX1|A0A0A0LWX1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G600200 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 6.3e-114
Identity = 202/223 (90.58%), Postives = 208/223 (93.27%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           MF+KL+KRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNR+HCK
Sbjct: 1   MFSKLTKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRVHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           AVFG       GFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDM LPC
Sbjct: 61  AVFG------EGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMTLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRL+HNGG+  HNGLKSVI HFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE
Sbjct: 121 GVLRLQHNGGHGYHNGLKSVISHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMP 224
           RIDTALQEGVGALKLLLS  L E+AR FNHEQKYKHIRLQTMP
Sbjct: 181 RIDTALQEGVGALKLLLSHSLFESARHFNHEQKYKHIRLQTMP 217

[Close]

BLAST of Carg06124 vs. TrEMBL
Match: tr|A0A2I4FSD8|A0A2I4FSD8_9ROSI (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Juglans regia OX=51240 GN=LOC109001646 PE=3 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 5.2e-100
Identity = 177/224 (79.02%), Postives = 195/224 (87.05%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           M N+LS+  FCTA + RPWLFVGLGNPG+K++GTRHNVGFE+ID FAES GI MN +HCK
Sbjct: 1   MLNRLSRHCFCTA-TPRPWLFVGLGNPGDKYKGTRHNVGFELIDAFAESEGISMNAVHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           A+FG       GFV ++PVF+AKPQTYMNLSGES GPLAAYYKLPLNRVLVFHDD+NLPC
Sbjct: 61  AIFG------QGFVDEVPVFIAKPQTYMNLSGESTGPLAAYYKLPLNRVLVFHDDLNLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRL H GG+ SHNGLKSVI HFRGNREF RLRIGIG+PPGQMDPKAFLLQKFN TAQE
Sbjct: 121 GVLRLHHKGGHGSHNGLKSVIDHFRGNREFPRLRIGIGKPPGQMDPKAFLLQKFNATAQE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 225
           RID AL+EGVG LKLLLS GL+E+AR FN EQKYKHIRLQTMPT
Sbjct: 181 RIDAALEEGVGVLKLLLSKGLTESARCFNREQKYKHIRLQTMPT 217

[Close]

BLAST of Carg06124 vs. TrEMBL
Match: tr|A0A059CQE8|A0A059CQE8_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_C01519 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.5e-99
Identity = 177/224 (79.02%), Postives = 197/224 (87.95%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           M + +++R FCT  + RPWLFVGLGNPG+K++GTRHNVGFEMID FAES GIPM+ +HCK
Sbjct: 1   MHSVIARRCFCTG-APRPWLFVGLGNPGDKYKGTRHNVGFEMIDAFAESQGIPMSTVHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           A+FG       GFVGD+PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDM+LPC
Sbjct: 61  AMFG------QGFVGDVPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMDLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRL+  GG+ SHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFN TA+E
Sbjct: 121 GVLRLQPKGGHGSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNQTARE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 225
           RID ALQEG  ALKL+LS GL+E+ R FN EQKYKHIRLQT+PT
Sbjct: 181 RIDVALQEGADALKLVLSKGLTESTRCFNMEQKYKHIRLQTLPT 217

[Close]

BLAST of Carg06124 vs. TrEMBL
Match: tr|A0A059CPZ2|A0A059CPZ2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_C01330 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 2.9e-98
Identity = 176/224 (78.57%), Postives = 196/224 (87.50%), Query Frame = 0


Query: 1   MFNKLSKRGFCTAISSRPWLFVGLGNPGEKFRGTRHNVGFEMIDVFAESVGIPMNRIHCK 60
           M + +++R FCT  + RPWLFVGLGNPG+K+RGTRHNVGFEMID FAES GI M+ +HCK
Sbjct: 1   MHSVIARRCFCTG-APRPWLFVGLGNPGDKYRGTRHNVGFEMIDAFAESQGILMSTVHCK 60

Query: 61  AVFGCLCIDMSGFVGDIPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMNLPC 120
           A+FG       GFVGD+PVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDM+LPC
Sbjct: 61  AMFG------QGFVGDVPVFLAKPQTYMNLSGESAGPLAAYYKLPLNRVLVFHDDMDLPC 120

Query: 121 GVLRLRHNGGNDSHNGLKSVIYHFRGNREFARLRIGIGRPPGQMDPKAFLLQKFNNTAQE 180
           GVLRL+  GG+ SHNGLKSVIYHF+GNREFARLRIGIGRPPGQMDPKAFLLQKFN TA+E
Sbjct: 121 GVLRLQPKGGHGSHNGLKSVIYHFQGNREFARLRIGIGRPPGQMDPKAFLLQKFNQTARE 180

Query: 181 RIDTALQEGVGALKLLLSLGLSETARRFNHEQKYKHIRLQTMPT 225
           RID ALQEG  ALKL+LS GL+E+ R FN EQKYKHIRLQT+PT
Sbjct: 181 RIDVALQEGADALKLVLSKGLTESTRCFNMEQKYKHIRLQTLPT 217

The following BLAST results are available for this feature:

BLAST of Carg06124 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961338.1	6.6e-123	96.88	peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita moschata] >XP_02296...	[more]
XP_022988207.1	3.3e-122	95.98	peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucurbita maxima] >XP_0229882...	[more]
XP_023532821.1	1.2e-121	95.98	peptidyl-tRNA hydrolase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pe...	[more]
XP_008451022.1	3.5e-116	91.48	PREDICTED: peptidyl-tRNA hydrolase, mitochondrial isoform X1 [Cucumis melo]	[more]
XP_022147698.1	1.5e-114	90.18	peptidyl-tRNA hydrolase, mitochondrial [Momordica charantia]	[more]

BLAST of Carg06124 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G19830.1	5.3e-90	69.82	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.1	1.1e-60	56.06	Peptidyl-tRNA hydrolase family protein	[more]
AT5G38290.2	1.3e-59	55.05	Peptidyl-tRNA hydrolase family protein	[more]
AT1G18440.1	1.7e-59	51.00	Peptidyl-tRNA hydrolase family protein	[more]

BLAST of Carg06124 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q6NLS8\|PTHM_ARATH	9.5e-89	69.82	Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19...	[more]
sp\|Q9M5P4\|CRS2_MAIZE	1.3e-61	55.45	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea m...	[more]
sp\|Q9LF14\|CRS2B_ARATH	2.1e-59	56.06	Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Ara...	[more]
sp\|Q9FKN4\|CRS2A_ARATH	2.3e-58	55.05	Chloroplastic group IIB intron splicing facilitator CRS2-A, chloroplastic OS=Ara...	[more]
sp\|Q5ZCL8\|CRS2_ORYSJ	3.0e-58	54.04	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Oryza...	[more]

BLAST of Carg06124 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BPZ7\|A0A1S3BPZ7_CUCME	2.3e-116	91.48	peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC...	[more]
tr\|A0A0A0LWX1\|A0A0A0LWX1_CUCSA	6.3e-114	90.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G600200 PE=3 SV=1	[more]
tr\|A0A2I4FSD8\|A0A2I4FSD8_9ROSI	5.2e-100	79.02	peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Juglans regia OX=51240 GN=L...	[more]
tr\|A0A059CQE8\|A0A059CQE8_EUCGR	1.5e-99	79.02	Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_C01519 PE=3 SV...	[more]
tr\|A0A059CPZ2\|A0A059CPZ2_EUCGR	2.9e-98	78.57	Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_C01330 PE=3 SV...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004045	aminoacyl-tRNA hydrolase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR018171	Pept_tRNA_hydro_CS
IPR036416	Pept_tRNA_hydro_sf
IPR001328	Pept_tRNA_hydro

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06124-RA	Carg06124-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001328	Peptidyl-tRNA hydrolase	PFAM	PF01195	Pept_tRNA_hydro	coord: 20..208 e-value: 1.3E-48 score: 165.0
IPR001328	Peptidyl-tRNA hydrolase	TIGRFAM	TIGR00447	TIGR00447	coord: 20..209 e-value: 1.1E-51 score: 173.2
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 1..220
IPR001328	Peptidyl-tRNA hydrolase	HAMAP	MF_00083	Pept_tRNA_hydro_bact	coord: 18..202 score: 30.24
IPR036416	Peptidyl-tRNA hydrolase superfamily	GENE3D	G3DSA:3.40.50.1470		coord: 4..212 e-value: 6.8E-67 score: 226.8
IPR036416	Peptidyl-tRNA hydrolase superfamily	SUPERFAMILY	SSF53178	Peptidyl-tRNA hydrolase-like	coord: 18..209
None	No IPR available	PANTHER	PTHR17224:SF4	PEPTIDYL-TRNA HYDROLASE, MITOCHONDRIAL	coord: 1..220
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01195	PEPT_TRNA_HYDROL_1	coord: 31..44
IPR018171	Peptidyl-tRNA hydrolase, conserved site	PROSITE	PS01196	PEPT_TRNA_HYDROL_2	coord: 130..140

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg06124	Cla011101	Watermelon (97103) v1	carwmB0018
Carg06124	Csa1G599570	Cucumber (Chinese Long) v2	carcuB0015
Carg06124	MELO3C015585	Melon (DHL92) v3.5.1	carmeB0019
Carg06124	ClCG03G014460	Watermelon (Charleston Gray)	carwcgB0024
Carg06124	CSPI01G29310	Wild cucumber (PI 183967)	carcpiB0015
Carg06124	Cucsa.142350	Cucumber (Gy14) v1	carcgyB0020
Carg06124	CmaCh10G010040	Cucurbita maxima (Rimu)	carcmaB0026
Carg06124	CmoCh10G010590	Cucurbita moschata (Rifu)	carcmoB0027
Carg06124	Lsi06G004030	Bottle gourd (USVL1VR-Ls)	carlsiB021
Carg06124	Cp4.1LG05g08460	Cucurbita pepo (Zucchini)	carcpeB0038
Carg06124	CsGy1G028550	Cucumber (Gy14) v2	carcgybB0014
Carg06124	MELO3C015585.2	Melon (DHL92) v3.6.1	carmedB0020
Carg06124	CsaV3_1G041710	Cucumber (Chinese Long) v3	carcucB0015
Carg06124	Cla97C03G064680	Watermelon (97103) v2	carwmbB0020
Carg06124	Bhi02G001444	Wax gourd	carwgoB0027

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg06124	Silver-seed gourd	carcarB017
Carg06124	Cucurbita maxima (Rimu)	carcmaB0027
Carg06124	Cucurbita moschata (Rifu)	carcmoB0028
Carg06124	Cucurbita pepo (Zucchini)	carcpeB0037