Carg05603 (gene) Silver-seed gourd

Overview
NameCarg05603
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCucurbita_argyrosperma_scaffold_046 : 1580976 .. 1582071 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCTTCTTCCCTTTCTTTGCTAGCTGATGCTTGCAGAAAATGGGGCTTTTTCCATATCAAAAACCATGGAATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCGTTCTTTGAAACTCTCAGAGTCTCTGGTCCTAACTTCTTAGCATCTGCTCAGAGTTCAGCAGATTCCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGGTACATACAATTCACAGCACATCTACTTCAAATCAGTATTGTTCTATTGTTCTAACAGTCTAATTTCATGTGCATAAAGTGAGATATTGCAAGAATATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATATTGATGAGCTTAGGGGAAGGTTTTGATACGAAATACCATGAATCTCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACACACCTCCAGAAACCTTGGGAGAAGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGCAGGGGATTGGGTCGATATAGCCCCATGTGATGAATCCGAATCCGAATCCGAATCCGACTCCGAAGCTCTTGTGGTGAACATTGGCGATCTTCTTCAAGCTTGGAGCAACGACAGGCTTAGGTCGTCTGAGCACAGAGTGGTTTTGCGGCGGCCGGCGATCAATCGGTTCTCGATTGCATTCTTCTGGGGGTTTGAGGACGAGAAGGTCGTGTCGGCGCCGGAGGAAGTGGTCGGCGACGGCGGTAAGAGACTGTATAAGCCATTTGTTTGCTCGGATTACCTGAAGTTCAGGGAGAATGACGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGACTTTGCTGGGAACGCAATTACTAAAGATTTTGAAGAACAATAA

mRNA sequence

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCTTCTTCCCTTTCTTTGCTAGCTGATGCTTGCAGAAAATGGGGCTTTTTCCATATCAAAAACCATGGAATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCGTTCTTTGAAACTCTCAGAGTCTCTGGTCCTAACTTCTTAGCATCTGCTCAGAGTTCAGCAGATTCCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATATTGATGAGCTTAGGGGAAGGTTTTGATACGAAATACCATGAATCTCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACACACCTCCAGAAACCTTGGGAGAAGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGCAGGGGATTGGGTCGATATAGCCCCATGTGATGAATCCGAATCCGAATCCGAATCCGACTCCGAAGCTCTTGTGGTGAACATTGGCGATCTTCTTCAAGCTTGGAGCAACGACAGGCTTAGGTCGTCTGAGCACAGAGTGGTTTTGCGGCGGCCGGCGATCAATCGGTTCTCGATTGCATTCTTCTGGGGGTTTGAGGACGAGAAGGTCGTGTCGGCGCCGGAGGAAGTGGTCGGCGACGGCGGTAAGAGACTGTATAAGCCATTTGTTTGCTCGGATTACCTGAAGTTCAGGGAGAATGACGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGACTTTGCTGGGAACGCAATTACTAAAGATTTTGAAGAACAATAA

Coding sequence (CDS)

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTGTCTCCTTCTTCCCTTTCTTTGCTAGCTGATGCTTGCAGAAAATGGGGCTTTTTCCATATCAAAAACCATGGAATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCTCTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCGTTCTTTGAAACTCTCAGAGTCTCTGGTCCTAACTTCTTAGCATCTGCTCAGAGTTCAGCAGATTCCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATATTGATGAGCTTAGGGGAAGGTTTTGATACGAAATACCATGAATCTCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAATTACACACCTCCAGAAACCTTGGGAGAAGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGCAGGGGATTGGGTCGATATAGCCCCATGTGATGAATCCGAATCCGAATCCGAATCCGACTCCGAAGCTCTTGTGGTGAACATTGGCGATCTTCTTCAAGCTTGGAGCAACGACAGGCTTAGGTCGTCTGAGCACAGAGTGGTTTTGCGGCGGCCGGCGATCAATCGGTTCTCGATTGCATTCTTCTGGGGGTTTGAGGACGAGAAGGTCGTGTCGGCGCCGGAGGAAGTGGTCGGCGACGGCGGTAAGAGACTGTATAAGCCATTTGTTTGCTCGGATTACCTGAAGTTCAGGGAGAATGACGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGACTTTGCTGGGAACGCAATTACTAAAGATTTTGAAGAACAATAA

Protein sequence

MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFSEILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFVCSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEQ
Homology
[Close]
BLAST of Carg05603 vs. NCBI nr
Match: XP_022959365.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 667.5 bits (1721), Expect = 2.3e-188
Identity = 337/338 (99.70%), Postives = 338/338 (100.00%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEQ 339
           CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE+
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 338

[Close]
BLAST of Carg05603 vs. NCBI nr
Match: XP_023547730.1 (gibberellin 20-oxidase-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.8 bits (1701), Expect = 4.9e-186
Identity = 325/338 (96.15%), Postives = 327/338 (96.75%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQEYGDKMTELSRKIIKNILMSLGEGFDT YHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDES          LVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDES--ESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300
           LLQAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV
Sbjct: 241 LLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEQ 339
           CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE+
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 336

[Close]
BLAST of Carg05603 vs. NCBI nr
Match: XP_023006611.1 (gibberellin 20-oxidase-like protein [Cucurbita maxima])

HSP 1 Score: 655.2 bits (1689), Expect = 1.2e-184
Identity = 322/338 (95.27%), Postives = 326/338 (96.45%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
           SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQ+YGDKMTELSRKIIKNILMSLGEGFDT YHESHFKNCHGYLRINNYTPPETLGEDE
Sbjct: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDES          LVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDES------ESEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300
           LLQAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGK+LYKPFV
Sbjct: 241 LLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEQ 339
           CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE+
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEEK 332

[Close]
BLAST of Carg05603 vs. NCBI nr
Match: XP_008458231.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo])

HSP 1 Score: 600.1 bits (1546), Expect = 4.6e-168
Identity = 293/333 (87.99%), Postives = 308/333 (92.49%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
           SLP ETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQ+SA+ LFNQKS HQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQEYG+KMTELSRKIIKN+L+S GEGF+ KYHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ES          LVVNIGD
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEES--------EALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP+E+VG+GGKR+YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITK 334
           CSDYLKFRENDERGKFEKVGFTVRDFAG  I K
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGKIINK 325

[Close]
BLAST of Carg05603 vs. NCBI nr
Match: XP_004138503.2 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] >KGN45657.1 hypothetical protein Csa_6G004480 [Cucumis sativus])

HSP 1 Score: 589.7 bits (1519), Expect = 6.2e-165
Identity = 288/328 (87.80%), Postives = 304/328 (92.68%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IYNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
           +LPSETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+ LFNQKS HQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQEYGDKMTELSRKIIKNILMS GEGF+ KYHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ES          LVVNIGD
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEES--------EALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300
           LLQAWSNDR+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE++V +GG+R+YKPFV
Sbjct: 241 LLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAG 329
           CSDYLKFRENDERGKFEKVGFTV +FAG
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVTNFAG 320

[Close]
BLAST of Carg05603 vs. TAIR10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 383.3 bits (983), Expect = 1.6e-106
Identity = 186/330 (56.36%), Postives = 251/330 (76.06%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MS   +S++LP+LD++QP+  S LS L++AC++WGFF++ NHGISK++++K+ S S ++F
Sbjct: 1   MSELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
             P E+KLK+GP+S    YTP +IASP+FE+L VSGP+F  SA++SAD LF      +  
Sbjct: 61  KAPLESKLKLGPIS----YTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELR 120

Query: 121 EILQEYGDKMTELSRKIIKNIL-MSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGED 180
           E +QEYG KM ELS+++IK +L M+LG+    + +++ F NCHGYLR+ NYTPP  + + 
Sbjct: 121 ETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQ 180

Query: 181 EE-PEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNI 240
           EE  EGLGMHTDMSC+TIVYQD  GGLQ++S  G W+DI PC++           LVVNI
Sbjct: 181 EELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCND----------FLVVNI 240

Query: 241 GDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKP 300
           GDL+QAWSN RLRSSEHRVVLR+  +NR S+AFF  FEDEKV+ AP+E+VG+G +R YK 
Sbjct: 241 GDLMQAWSNGRLRSSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKS 300

Query: 301 FVCSDYLKFRENDERGKFEKVGFTVRDFAG 329
           F CS+YLKFR+++E GKFEK+GFTV+DFAG
Sbjct: 301 FKCSEYLKFRQSNEEGKFEKIGFTVKDFAG 315

[Close]
BLAST of Carg05603 vs. TAIR10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 363.2 bits (931), Expect = 1.7e-100
Identity = 186/332 (56.02%), Postives = 237/332 (71.39%), Query Frame = 0 

Query: 7   SVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSET 66
           S+ELP+ DIS+PLS SSL+ L DAC++WGFF++ NHG+S+D+Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFSEILQEY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA+SS D+  +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDEEP---- 186
           G+KMT+L  KI+K IL S G+    KY+ES F NCHGY RINNYT P    +D       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 ---EGLGMHTDMSCVTIVYQDQTGGLQVKSSAG-DWVDIAPCDESXXXXXXXXXXLVVNI 246
              EGLGMHTDMSC+TIV QD  GGLQV++  G   +DI P DE+          LVVN+
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA----------LVVNV 241

Query: 247 GDLLQAWSNDRLRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKVVSAPEEVVGD-GGKRL 306
           GDLL AW+N RLRSS+HRV+L+R     NRFS+AFFW F+D KVV AP+EVVG   G R+
Sbjct: 242 GDLLHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRV 301

Query: 307 YKPFVCSDYLKFRENDERGKFEKVGFTVRDFA 328
           ++ F C DYL+FRE++E+GKFEKVG TV DFA
Sbjct: 302 FRSFKCGDYLRFRESNEKGKFEKVGDTVEDFA 319

[Close]
BLAST of Carg05603 vs. TAIR10
Match: AT1G80330.1 (gibberellin 3-oxidase 4)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-33
Identity = 102/317 (32.18%), Postives = 150/317 (47.32%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           +S       +P++D+S   +P   +L+ DA + WG F I NHGIS+ + + + S S  +F
Sbjct: 40  VSFDAVEESIPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLF 99

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPH--- 120
            +PSE KL+        +       SPFFE    S       A  S  + FN   PH   
Sbjct: 100 DMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWS--EGFTIADDSYRNHFNTLWPHDHT 159

Query: 121 QFSEILQEYGDKMTELSRKIIKNILMSLGEGFDT-----KYHESHFKNCHGYLRINNYTP 180
           ++  I+QEY D+M +L+ +++  IL SLG   +      K  +S  K   G +R+N+Y  
Sbjct: 160 KYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-- 219

Query: 181 PETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXX 240
                E E   GL  HTD + +TI++Q  TGGLQV      WV + P             
Sbjct: 220 -PVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA----------PG 279

Query: 241 XLVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP-EEVVGDG 300
            LVVNIGDL    SN ++ S  HR  +     +R SIA+ WG     V  AP  ++ G  
Sbjct: 280 VLVVNIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPA 337

Query: 301 GKRLYKPFVCSDYLKFR 309
              LY+     +YL+ +
Sbjct: 340 EPSLYRSITWKEYLQIK 337

[Close]
BLAST of Carg05603 vs. TAIR10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 137.5 bits (345), Expect = 1.5e-32
Identity = 104/310 (33.55%), Postives = 152/310 (49.03%), Query Frame = 0 

Query: 10  LPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLK 69
           +P++DIS     S    + DA  +WGFF + NHG+S ++   + + ++  F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  IGPLSSLNT-------YTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFSEI 129
                SL+T       ++PH  A    E        F++ A++S      Q  P      
Sbjct: 122 FSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAEAS------QLWPDSCRSE 181

Query: 130 LQEYGDKMTELSRKIIKNILMSLGEGFDT----KYHESHFKNCHGYLRIN-NYTPPETLG 189
             EY   M E ++ ++K +L  LGE  +     K  ES F    G  RIN NY P     
Sbjct: 182 TLEY---MNE-TKPLVKKLLRFLGENLNVKELDKTKESFFM---GSTRINLNYYP--ICP 241

Query: 190 EDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKS-SAGDWVDIAPCDESXXXXXXXXXXLVV 249
             E   G+G H+D+S +TI+ QD+ GGL V+S + G WV + P   S          LV+
Sbjct: 242 NPELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPISGS----------LVI 301

Query: 250 NIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLY 307
           NIGD +Q  SN R +S EHR VL   + NR S+  F   + E V+    EV+ +G K +Y
Sbjct: 302 NIGDAMQIMSNGRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVY 343

[Close]
BLAST of Carg05603 vs. TAIR10
Match: AT3G13610.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 133.7 bits (335), Expect = 2.2e-31
Identity = 96/316 (30.38%), Postives = 161/316 (50.95%), Query Frame = 0 

Query: 3   VSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSL 62
           V++    +P++D+S P        + DA  KWGFF + NHG+  ++ + + + +++ F+L
Sbjct: 55  VNETDEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 114

Query: 63  PSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD-----SLF--NQKS 122
           P E K K    +SL+T            T+R  G +F   A+ + +     SLF  ++  
Sbjct: 115 PVEEKRKFTKENSLST------------TVRF-GTSFSPLAEQALEWKDYLSLFFVSEAE 174

Query: 123 PHQF-SEILQEYGDKMTELSRKIIKNILMSLGEGFDTK-YHESHFKNCHGYLRIN-NYTP 182
             QF  +I +    +    S+K+++ +L  LG+  + K   E+      G +R+N NY P
Sbjct: 175 AEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYP 234

Query: 183 PETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKS-SAGDWVDIAPCDESXXXXXXXX 242
                  +   G+G H+D+S +TI+ QDQ GGL V+S ++G+WV + P   S        
Sbjct: 235 --ICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGS-------- 294

Query: 243 XXLVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDG 302
              V+NIGD +Q  SN   +S EHR VL     NR S+  F   + E V+    EV+ +G
Sbjct: 295 --FVINIGDAMQIMSNGLYKSVEHR-VLANGYNNRISVPIFVNPKPESVIGPLPEVIANG 344

Query: 303 GKRLYKPFVCSDYLKF 308
            + +Y+  + SDY+K+
Sbjct: 355 EEPIYRDVLYSDYVKY 344

[Close]
BLAST of Carg05603 vs. Swiss-Prot
Match: sp|F4KBY0|GAOXL_ARATH (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 3.1e-99
Identity = 186/332 (56.02%), Postives = 237/332 (71.39%), Query Frame = 0 

Query: 7   SVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSET 66
           S+ELP+ DIS+PLS SSL+ L DAC++WGFF++ NHG+S+D+Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFSEILQEY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA+SS D+  +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDEEP---- 186
           G+KMT+L  KI+K IL S G+    KY+ES F NCHGY RINNYT P    +D       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 ---EGLGMHTDMSCVTIVYQDQTGGLQVKSSAG-DWVDIAPCDESXXXXXXXXXXLVVNI 246
              EGLGMHTDMSC+TIV QD  GGLQV++  G   +DI P DE+          LVVN+
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEA----------LVVNV 241

Query: 247 GDLLQAWSNDRLRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKVVSAPEEVVGD-GGKRL 306
           GDLL AW+N RLRSS+HRV+L+R     NRFS+AFFW F+D KVV AP+EVVG   G R+
Sbjct: 242 GDLLHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRV 301

Query: 307 YKPFVCSDYLKFRENDERGKFEKVGFTVRDFA 328
           ++ F C DYL+FRE++E+GKFEKVG TV DFA
Sbjct: 302 FRSFKCGDYLRFRESNEKGKFEKVGDTVEDFA 319

[Close]
BLAST of Carg05603 vs. Swiss-Prot
Match: sp|Q9C971|G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.9e-32
Identity = 102/317 (32.18%), Postives = 150/317 (47.32%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           +S       +P++D+S   +P   +L+ DA + WG F I NHGIS+ + + + S S  +F
Sbjct: 40  VSFDAVEESIPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLF 99

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPH--- 120
            +PSE KL+        +       SPFFE    S       A  S  + FN   PH   
Sbjct: 100 DMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWS--EGFTIADDSYRNHFNTLWPHDHT 159

Query: 121 QFSEILQEYGDKMTELSRKIIKNILMSLGEGFDT-----KYHESHFKNCHGYLRINNYTP 180
           ++  I+QEY D+M +L+ +++  IL SLG   +      K  +S  K   G +R+N+Y  
Sbjct: 160 KYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-- 219

Query: 181 PETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXX 240
                E E   GL  HTD + +TI++Q  TGGLQV      WV + P             
Sbjct: 220 -PVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA----------PG 279

Query: 241 XLVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP-EEVVGDG 300
            LVVNIGDL    SN ++ S  HR  +     +R SIA+ WG     V  AP  ++ G  
Sbjct: 280 VLVVNIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPA 337

Query: 301 GKRLYKPFVCSDYLKFR 309
              LY+     +YL+ +
Sbjct: 340 EPSLYRSITWKEYLQIK 337

[Close]
BLAST of Carg05603 vs. Swiss-Prot
Match: sp|Q9C899|F6H2_ARATH (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.7e-31
Identity = 104/310 (33.55%), Postives = 152/310 (49.03%), Query Frame = 0 

Query: 10  LPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLK 69
           +P++DIS     S    + DA  +WGFF + NHG+S ++   + + ++  F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  IGPLSSLNT-------YTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFSEI 129
                SL+T       ++PH  A    E        F++ A++S      Q  P      
Sbjct: 122 FSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAEAS------QLWPDSCRSE 181

Query: 130 LQEYGDKMTELSRKIIKNILMSLGEGFDT----KYHESHFKNCHGYLRIN-NYTPPETLG 189
             EY   M E ++ ++K +L  LGE  +     K  ES F    G  RIN NY P     
Sbjct: 182 TLEY---MNE-TKPLVKKLLRFLGENLNVKELDKTKESFFM---GSTRINLNYYP--ICP 241

Query: 190 EDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKS-SAGDWVDIAPCDESXXXXXXXXXXLVV 249
             E   G+G H+D+S +TI+ QD+ GGL V+S + G WV + P   S          LV+
Sbjct: 242 NPELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPISGS----------LVI 301

Query: 250 NIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLY 307
           NIGD +Q  SN R +S EHR VL   + NR S+  F   + E V+    EV+ +G K +Y
Sbjct: 302 NIGDAMQIMSNGRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVY 343

[Close]
BLAST of Carg05603 vs. Swiss-Prot
Match: sp|Q9LHN8|F6H1_ARATH (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 4.0e-30
Identity = 96/316 (30.38%), Postives = 161/316 (50.95%), Query Frame = 0 

Query: 3   VSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSL 62
           V++    +P++D+S P        + DA  KWGFF + NHG+  ++ + + + +++ F+L
Sbjct: 55  VNETDEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 114

Query: 63  PSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSAD-----SLF--NQKS 122
           P E K K    +SL+T            T+R  G +F   A+ + +     SLF  ++  
Sbjct: 115 PVEEKRKFTKENSLST------------TVRF-GTSFSPLAEQALEWKDYLSLFFVSEAE 174

Query: 123 PHQF-SEILQEYGDKMTELSRKIIKNILMSLGEGFDTK-YHESHFKNCHGYLRIN-NYTP 182
             QF  +I +    +    S+K+++ +L  LG+  + K   E+      G +R+N NY P
Sbjct: 175 AEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYP 234

Query: 183 PETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKS-SAGDWVDIAPCDESXXXXXXXX 242
                  +   G+G H+D+S +TI+ QDQ GGL V+S ++G+WV + P   S        
Sbjct: 235 --ICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGS-------- 294

Query: 243 XXLVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDG 302
              V+NIGD +Q  SN   +S EHR VL     NR S+  F   + E V+    EV+ +G
Sbjct: 295 --FVINIGDAMQIMSNGLYKSVEHR-VLANGYNNRISVPIFVNPKPESVIGPLPEVIANG 344

Query: 303 GKRLYKPFVCSDYLKF 308
            + +Y+  + SDY+K+
Sbjct: 355 EEPIYRDVLYSDYVKY 344

[Close]
BLAST of Carg05603 vs. Swiss-Prot
Match: sp|Q39110|GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 130.2 bits (326), Expect = 4.4e-29
Identity = 97/325 (29.85%), Postives = 163/325 (50.15%), Query Frame = 0 

Query: 2   SVSKASVELPILDISQPLS-PSSL----SLLADACRKWGFFHIKNHGISKDIYNKLHSFS 61
           S++   +++P++D+   LS PSS      L+++AC+K GFF + NHGIS+++ +  H ++
Sbjct: 53  SINVLELDVPLIDLQNLLSDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYT 112

Query: 62  NEIFSLPSETKLKI-----GPLSSLNTYTPHFIAS-PFFETLRVSGPNFLASAQSSADSL 121
           +  F +P   K ++       +   +++T  F    P+ ETL     + ++ ++S  D  
Sbjct: 113 SRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKSVQDYF 172

Query: 122 FNQKSP--HQFSEILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRIN 181
            +        F ++ QEY + M+ LS KI++ + +SLG   D  Y    F+     +R+N
Sbjct: 173 CDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRD--YFREFFEENDSIMRLN 232

Query: 182 NYTPPETLGEDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXX 241
            Y P     + +   G G H D + +TI++QD   GLQV      W  I P  ++     
Sbjct: 233 YYPP---CIKPDLTLGTGPHCDPTSLTILHQDHVNGLQV-FVENQWRSIRPNPKA----- 292

Query: 242 XXXXXLVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVV 301
                 VVNIGD   A SNDR +S  HR V+   +  R S+AFF   + ++VV+ P E++
Sbjct: 293 -----FVVNIGDTFMALSNDRYKSCLHRAVVNSES-ERKSLAFFLCPKKDRVVTPPRELL 352

Query: 302 GDGGKRLYKPFVCSDYLKFRENDER 314
                R Y  F  S +L+F +   R
Sbjct: 353 DSITSRRYPDFTWSMFLEFTQKHYR 360

[Close]
BLAST of Carg05603 vs. TrEMBL
Match: tr|A0A1S3C8M6|A0A1S3C8M6_CUCME (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LOC103497717 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 3.0e-168
Identity = 293/333 (87.99%), Postives = 308/333 (92.49%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
           SLP ETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQ+SA+ LFNQKS HQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQEYG+KMTELSRKIIKN+L+S GEGF+ KYHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ES          LVVNIGD
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEES--------EALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP+E+VG+GGKR+YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITK 334
           CSDYLKFRENDERGKFEKVGFTVRDFAG  I K
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGKIINK 325

[Close]
BLAST of Carg05603 vs. TrEMBL
Match: tr|A0A0A0KB92|A0A0A0KB92_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004480 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 4.1e-165
Identity = 288/328 (87.80%), Postives = 304/328 (92.68%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLS LADAC+KWGFFHIKNHGISK+IYNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
           +LPSETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQ+SA+ LFNQKS HQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQEYGDKMTELSRKIIKNILMS GEGF+ KYHE HFKNCHGYLRINNYTPPETL +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI PC+ES          LVVNIGD
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEES--------EALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300
           LLQAWSNDR+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE++V +GG+R+YKPFV
Sbjct: 241 LLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAG 329
           CSDYLKFRENDERGKFEKVGFTV +FAG
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVTNFAG 320

[Close]
BLAST of Carg05603 vs. TrEMBL
Match: tr|A0A2P4JT81|A0A2P4JT81_QUESU (Gibberellin 20-oxidase-like protein OS=Quercus suber OX=58331 GN=CFP56_37011 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 2.5e-130
Identity = 233/328 (71.04%), Postives = 278/328 (84.76%), Query Frame = 0 

Query: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MS S+ S+ELPILDISQPL+PS LS LA+AC+KWGFFHI NHGISKD+++KL+  SN++F
Sbjct: 1   MSESQTSIELPILDISQPLNPSFLSSLAEACKKWGFFHIINHGISKDLFSKLYLLSNDLF 60

Query: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120
           +LPS+TKLK+GP SS+ TYTPHFIASP+FE+LRVSGPNFL SAQSSA++LF Q++  +FS
Sbjct: 61  NLPSDTKLKVGPFSSIKTYTPHFIASPYFESLRVSGPNFLDSAQSSANALFVQQN-SEFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180
           EILQEYG KM ELS+ IIK +LMSL +GF  K +ES FKNCHGY+RINNY+PPE+L  + 
Sbjct: 121 EILQEYGRKMEELSKNIIKVLLMSLKDGFGKKDYESEFKNCHGYMRINNYSPPESL--EV 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGD 240
           E EGLGMHTDMSCVTIVYQD+ GGLQVKS  G W+DI PC+ +          LVVNIGD
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDEIGGLQVKSKEGKWMDIRPCEGT----------LVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300
           +LQAWSN++ RSSEHRVVL++PA NRFS+AFFW FEDEKV+ AP+EVVG+G  RLY PFV
Sbjct: 241 MLQAWSNEKFRSSEHRVVLKQPA-NRFSLAFFWCFEDEKVIIAPDEVVGEGNVRLYNPFV 300

Query: 301 CSDYLKFRENDERGKFEKVGFTVRDFAG 329
           C DYLKFRE++ERGKFEKVGFTVRDFAG
Sbjct: 301 CLDYLKFRESNERGKFEKVGFTVRDFAG 314

[Close]
BLAST of Carg05603 vs. TrEMBL
Match: tr|A0A2C9VT09|A0A2C9VT09_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_05G034600 PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 5.6e-130
Identity = 235/326 (72.09%), Postives = 279/326 (85.58%), Query Frame = 0 

Query: 5   KASVELPILDISQP-LSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLP 64
           K SVELPILDIS+P L PSSLS LA+AC+KWGFFHIKNHGISK +YNK++S+S ++F+LP
Sbjct: 6   KTSVELPILDISKPLLHPSSLSSLAEACKKWGFFHIKNHGISKQLYNKIYSYSQDMFNLP 65

Query: 65  SETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFSEIL 124
           S+ KL++GP SSL TYTPHFIASPFFE+LRVSGPNF ASAQSSA+ LFNQ+S  +FSE L
Sbjct: 66  SDAKLELGPSSSLKTYTPHFIASPFFESLRVSGPNFFASAQSSANVLFNQQS-CEFSEAL 125

Query: 125 QEYGDKMTELSRKIIKNILMSLGEGFD-TKYHESHFKNCHGYLRINNYTPPETLGEDEEP 184
           QEYG+KMTELS++II+ +L+SL +G D  KY+ES FKN HGYLRI NYTPP+ L  D+E 
Sbjct: 126 QEYGNKMTELSKRIIEILLLSLEDGLDHKKYYESEFKNGHGYLRIINYTPPKCL--DDEV 185

Query: 185 EGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGDLL 244
           EGLGMHTDMSCVTIVYQDQ GGLQVKS  G W+DI+PC+E+          LVVNIGD+L
Sbjct: 186 EGLGMHTDMSCVTIVYQDQIGGLQVKSKQGKWMDISPCEET----------LVVNIGDML 245

Query: 245 QAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFVCS 304
           QAWSN++ RSSEHRVVL++P +NRFS+AFFW FEDEK++ AP+EVVG+G  R+YKPFVCS
Sbjct: 246 QAWSNEKFRSSEHRVVLKQP-VNRFSLAFFWCFEDEKMILAPDEVVGEGHARIYKPFVCS 305

Query: 305 DYLKFRENDERGKFEKVGFTVRDFAG 329
           DYLKFRE+ ERGKFEKVGFTVRDFAG
Sbjct: 306 DYLKFRESSERGKFEKVGFTVRDFAG 317

[Close]
BLAST of Carg05603 vs. TrEMBL
Match: tr|B9GQ95|B9GQ95_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_002G159500v3 PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 2.3e-128
Identity = 227/322 (70.50%), Postives = 269/322 (83.54%), Query Frame = 0 

Query: 7   SVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSET 66
           S ELPILDISQPL PS+L+ LA+AC++WGFF I NHGISK++YNKL+S S ++F LP+ET
Sbjct: 6   SAELPILDISQPLQPSALASLAEACQEWGFFRISNHGISKELYNKLYSLSQQLFGLPAET 65

Query: 67  KLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFSEILQEY 126
           KL++GP SS+NTYTPHFIASPFFE LRV GPNF ASAQSSAD+LF Q+S  +F   LQEY
Sbjct: 66  KLELGPSSSINTYTPHFIASPFFECLRVFGPNFFASAQSSADTLFGQQS-FEFRAALQEY 125

Query: 127 GDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDEEPEGLG 186
           G KMTELS++I++ + MSLGEGFD KY+ES FKNCHGYLRI NY PP+  G  +E EGLG
Sbjct: 126 GTKMTELSKRIVEILSMSLGEGFDNKYYESEFKNCHGYLRIINYNPPK--GLVDEVEGLG 185

Query: 187 MHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESXXXXXXXXXXLVVNIGDLLQAWS 246
           MHTDMSCVTIVYQD+ GGLQVKS  G W+DI+P +E+          LVVNIGDLLQAWS
Sbjct: 186 MHTDMSCVTIVYQDEVGGLQVKSREGKWMDISPGEET----------LVVNIGDLLQAWS 245

Query: 247 NDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFVCSDYLK 306
           ND+LRSSEHR+VL++P +NR S+AFFW FEDEKV+ AP EVVG+G  R+Y+PFVCSDYLK
Sbjct: 246 NDKLRSSEHRIVLKKP-VNRLSLAFFWCFEDEKVIMAPNEVVGEGNARIYEPFVCSDYLK 305

Query: 307 FRENDERGKFEKVGFTVRDFAG 329
           FRE+ ERGKFEKVGFTV+DFAG
Sbjct: 306 FRESSERGKFEKVGFTVKDFAG 313

The following BLAST results are available for this feature:
BLAST of Carg05603 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022959365.12.3e-18899.70gibberellin 20-oxidase-like protein [Cucurbita moschata][more]
XP_023547730.14.9e-18696.15gibberellin 20-oxidase-like protein [Cucurbita pepo subsp. pepo][more]
XP_023006611.11.2e-18495.27gibberellin 20-oxidase-like protein [Cucurbita maxima][more]
XP_008458231.14.6e-16887.99PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo][more]
XP_004138503.26.2e-16587.80PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus] ... [more]
BLAST of Carg05603 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G23340.11.6e-10656.362-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT5G51310.11.7e-10056.022-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G80330.11.1e-3332.18gibberellin 3-oxidase 4[more]
AT1G55290.11.5e-3233.552-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT3G13610.12.2e-3130.382-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
BLAST of Carg05603 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|F4KBY0|GAOXL_ARATH3.1e-9956.02Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310... [more]
sp|Q9C971|G3OX4_ARATH1.9e-3232.18Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ... [more]
sp|Q9C899|F6H2_ARATH2.7e-3133.55Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S... [more]
sp|Q9LHN8|F6H1_ARATH4.0e-3030.38Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S... [more]
sp|Q39110|GAOX1_ARATH4.4e-2929.85Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2[more]
BLAST of Carg05603 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3C8M6|A0A1S3C8M6_CUCME3.0e-16887.99probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LO... [more]
tr|A0A0A0KB92|A0A0A0KB92_CUCSA4.1e-16587.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004480 PE=3 SV=1[more]
tr|A0A2P4JT81|A0A2P4JT81_QUESU2.5e-13071.04Gibberellin 20-oxidase-like protein OS=Quercus suber OX=58331 GN=CFP56_37011 PE=... [more]
tr|A0A2C9VT09|A0A2C9VT09_MANES5.6e-13072.09Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_05G034600 PE=3 SV=... [more]
tr|B9GQ95|B9GQ95_POPTR2.3e-12870.50Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_002G159500v3 PE=... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR026992DIOX_N
IPR027443IPNS-like
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05603-RACarg05603-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 166..272
e-value: 5.8E-19
score: 68.4
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 161..275
score: 11.205
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 1..307
e-value: 2.9E-69
score: 236.0
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 10..72
e-value: 9.9E-15
score: 55.2
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 2..330
NoneNo IPR availablePANTHERPTHR10209:SF212OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 2..330
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 7..311