CSPI06G00480 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G00480
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr6 : 360067 .. 361181 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TATAGGCTAGCTAGCTATAGACAAACATGTGTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCTTTGTCTCCATCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGGTTTTTCCACATAAAAAACCATGGGATCTCCAAAGAAATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAACCTCCCTTCAGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCCGTAAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAATTCAGCAGAGTTCCTCTTTAACCAAAAGAGCTCTCACCAGTTCAGGTACACACAATTCACATCACAGCACATCCACATCCACATCCACTTCAGAATCACTATCTCTGTAATCTAAGATCATTACTTCGTTTATCCTAACAATCTACTTTCATGTGGTTAAAGTGAGATATTGCAAGAATATGGAGATAAAATGACAGAGCTATCAAGGAAAATTATCAAGAACATACTGATGAGCTTTGGTGAAGGTTTTGAGAAGAAATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAACTACACACCTCCAGAAACGTTAGAAGACGATGAAGAAGTAGAAGGACTTGGAATGCATACAGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTGGACATAAACCCATGTGAAGAATCAGAAGCACTGGTGGTGAATATTGGGGACTTGCTTCAAGCTTGGAGCAACGACAGGGTGAGGTCGTCGGAGCACAGAGTGGTTTTGAGGCGGCCGGCGATCAACCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGCCGGAGGAGTTGGTGGAAGAGGGCGGTGAGAGGATGTATAAGCCATTTGTGTGCTCGGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACCGTCACAAACTTTGCTGGGAAAAAGGATTAA

mRNA sequence

ATGTGTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCTTTGTCTCCATCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGGTTTTTCCACATAAAAAACCATGGGATCTCCAAAGAAATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAACCTCCCTTCAGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCCGTAAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAATTCAGCAGAGTTCCTCTTTAACCAAAAGAGCTCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGATAAAATGACAGAGCTATCAAGGAAAATTATCAAGAACATACTGATGAGCTTTGGTGAAGGTTTTGAGAAGAAATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAACTACACACCTCCAGAAACGTTAGAAGACGATGAAGAAGTAGAAGGACTTGGAATGCATACAGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTGGACATAAACCCATGTGAAGAATCAGAAGCACTGGTGGTGAATATTGGGGACTTGCTTCAAGCTTGGAGCAACGACAGGGTGAGGTCGTCGGAGCACAGAGTGGTTTTGAGGCGGCCGGCGATCAACCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGCCGGAGGAGTTGGTGGAAGAGGGCGGTGAGAGGATGTATAAGCCATTTGTGTGCTCGGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACCGTCACAAACTTTGCTGGGAAAAAGGATTAA

Coding sequence (CDS)

ATGTGTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCTTTGTCTCCATCTTCCCTTTCTTCACTAGCTGATGCTTGCAAAAAATGGGGGTTTTTCCACATAAAAAACCATGGGATCTCCAAAGAAATCTACAACAAACTCCATTCCTTCTCCAATGAAATTTTCAACCTCCCTTCAGAAACCAAGCTCAAAATTGGCCCCCTCTCTTCCGTAAACACTTACACTCCCCATTTCATAGCCTCCCCATTCTTTGAAACCCTCAGAGTCTCTGGTCCAAACTTCTTAGCCTCTGCTCAGAATTCAGCAGAGTTCCTCTTTAACCAAAAGAGCTCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGATAAAATGACAGAGCTATCAAGGAAAATTATCAAGAACATACTGATGAGCTTTGGTGAAGGTTTTGAGAAGAAATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTGAGAATAAACAACTACACACCTCCAGAAACGTTAGAAGACGATGAAGAAGTAGAAGGACTTGGAATGCATACAGATATGAGCTGCGTGACCATTGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCAAGCGAAGGGAAATGGGTGGACATAAACCCATGTGAAGAATCAGAAGCACTGGTGGTGAATATTGGGGACTTGCTTCAAGCTTGGAGCAACGACAGGGTGAGGTCGTCGGAGCACAGAGTGGTTTTGAGGCGGCCGGCGATCAACCGATTCTCGATTGCTTTCTTTTGGGGTTTTGAGGACGAGAAGGTGGTGTCAGCGCCGGAGGAGTTGGTGGAAGAGGGCGGTGAGAGGATGTATAAGCCATTTGTGTGCTCGGATTATTTGAAGTTCAGGGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACCGTCACAAACTTTGCTGGGAAAAAGGATTAA

Homology

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BLAST of CSPI06G00480 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 8.9e-32
Identity = 97/316 (30.70%), Postives = 167/316 (52.85%), Query Frame = 1


Query: 3   VSKASVELPILDISQPLS-PSSLSS----LADACKKWGFFHIKNHGISKEIYNKLHSFSN 62
           ++   +++P++D+   LS PSS       +++ACKK GFF + NHGIS+E+ +  H +++
Sbjct: 54  INVLELDVPLIDLQNLLSDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYTS 113

Query: 63  EIFNLPSETKLKI-----GPLSSVNTYTPHFIAS-PFFETLRVSGPNFLASAQNSAEFLF 122
             F++P   K ++       +   +++T  F    P+ ETL     + ++ +++  ++  
Sbjct: 114 RFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKSVQDYFC 173

Query: 123 NQ--KSSHQFSEILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINN 182
           +        F ++ QEY + M+ LS KI++  L+    G ++ Y    F+     +R+N 
Sbjct: 174 DALGHGFQPFGKVYQEYCEAMSSLSLKIME--LLGLSLGVKRDYFREFFEENDSIMRLNY 233

Query: 183 YTPPETLEDDEEVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVN 242
           Y  P  ++ D  + G G H D + +TI++QD   GLQV   E +W  I P    +A VVN
Sbjct: 234 Y--PPCIKPDLTL-GTGPHCDPTSLTILHQDHVNGLQV-FVENQWRSIRP--NPKAFVVN 293

Query: 243 IGDLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYK 302
           IGD   A SNDR +S  HR V+   +  R S+AFF   + ++VV+ P EL++    R Y 
Sbjct: 294 IGDTFMALSNDRYKSCLHRAVVNSES-ERKSLAFFLCPKKDRVVTPPRELLDSITSRRYP 353

Query: 303 PFVCSDYLKFRENDER 306
            F  S +L+F +   R
Sbjct: 354 DFTWSMFLEFTQKHYR 360

[Close]

BLAST of CSPI06G00480 vs. Swiss-Prot
Match: F6H1_ARATH (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.6e-31
Identity = 95/308 (30.84%), Postives = 158/308 (51.30%), Query Frame = 1


Query: 3   VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNL 62
           V++    +P++D+S P       ++ DA +KWGFF + NHG+  E+ + + + +++ FNL
Sbjct: 55  VNETDEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 114

Query: 63  PSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAE-------FLFNQKS 122
           P E K K    +S++T            T+R  G +F   A+ + E       F  ++  
Sbjct: 115 PVEEKRKFTKENSLST------------TVRF-GTSFSPLAEQALEWKDYLSLFFVSEAE 174

Query: 123 SHQF-SEILQEYGDKMTELSRKIIKNILMSFGEGFE-KKYHEPHFKNCHGYLRIN-NYTP 182
           + QF  +I +    +    S+K+++ +L   G+    K+  E       G +R+N NY P
Sbjct: 175 AEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYP 234

Query: 183 PETLEDDEEVEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDINPCEESEALVVNIG 242
                + +   G+G H+D+S +TI+ QDQ GGL V+S + G WV + P   S   V+NIG
Sbjct: 235 --ICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGS--FVINIG 294

Query: 243 DLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPF 300
           D +Q  SN   +S EHR VL     NR S+  F   + E V+    E++  G E +Y+  
Sbjct: 295 DAMQIMSNGLYKSVEHR-VLANGYNNRISVPIFVNPKPESVIGPLPEVIANGEEPIYRDV 344

[Close]

BLAST of CSPI06G00480 vs. Swiss-Prot
Match: G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.6e-31
Identity = 101/301 (33.55%), Postives = 149/301 (49.50%), Query Frame = 1


Query: 10  LPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSETKLK 69
           +P++D+S   +P   + + DA K WG F I NHGIS+++ + + S S  +F++PSE KL+
Sbjct: 49  IPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERKLE 108

Query: 70  IGPL-SSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSH---QFSEILQE 129
                  V+ Y    I SPFFE    S       A +S    FN    H   ++  I+QE
Sbjct: 109 AASSDKGVSGYGEPRI-SPFFEKKMWS--EGFTIADDSYRNHFNTLWPHDHTKYCGIIQE 168

Query: 130 YGDKMTELSRKIIKNILMSFGEGFE-----KKYHEPHFKNCHGYLRINNYTPPETLEDDE 189
           Y D+M +L+ +++  IL S G   E      K  +   K   G +R+N+Y       + E
Sbjct: 169 YVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY---PVCPEPE 228

Query: 190 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 249
              GL  HTD + +TI++Q  TGGLQV   E  WV + P      LVVNIGDL    SN 
Sbjct: 229 RAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA--PGVLVVNIGDLFHILSNG 288

Query: 250 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP-EELVEEGGERMYKPFVCSDYLKF 301
           ++ S  HR  +     +R SIA+ WG     V  AP  +L       +Y+     +YL+ 
Sbjct: 289 KIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEYLQI 337

[Close]

BLAST of CSPI06G00480 vs. Swiss-Prot
Match: F6H2_ARATH (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.8e-31
Identity = 96/299 (32.11%), Postives = 153/299 (51.17%), Query Frame = 1


Query: 10  LPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSETKLK 69
           +P++DIS     S   ++ DA ++WGFF + NHG+S E+   + + ++  F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  IGPLSSVNT-------YTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEI 129
                S++T       ++PH  A    E        F++ A+  A  L+      +  E 
Sbjct: 122 FSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAE--ASQLWPDSCRSETLEY 181

Query: 130 LQEYGDKMTELSRKIIKNILMSFGEGFE-KKYHEPHFKNCHGYLRIN-NYTPPETLEDDE 189
           + E        ++ ++K +L   GE    K+  +       G  RIN NY P     + E
Sbjct: 182 MNE--------TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYP--ICPNPE 241

Query: 190 EVEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDINPCEESEALVVNIGDLLQAWSN 249
              G+G H+D+S +TI+ QD+ GGL V+S + G+WV + P   S +LV+NIGD +Q  SN
Sbjct: 242 LTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI--SGSLVINIGDAMQIMSN 301

Query: 250 DRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLK 299
            R +S EHR VL   + NR S+  F   + E V+    E++E G + +YK  + +DY+K
Sbjct: 302 GRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDILYTDYVK 343

[Close]

BLAST of CSPI06G00480 vs. Swiss-Prot
Match: AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.7e-30
Identity = 88/305 (28.85%), Postives = 156/305 (51.15%), Query Frame = 1


Query: 7   SVELPILDIS-QPLSPSS------LSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEI 66
           S +LP++D S Q L P S       + +  A + +G F      +S E+   +     ++
Sbjct: 4   SFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDL 63

Query: 67  FNLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQF 126
           F LP  TK +       + Y  H +    +E+L ++  N L    +  + L+    +   
Sbjct: 64  FELPIPTKQRNVSSKLFHGYLCHNL----YESLGINDANVLEKVNDFTQQLWPDHGNKSI 123

Query: 127 SEILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDD 186
           SE +  + +++ EL   + + I+ SFG    +KY + H  + +   R+  YT P   +DD
Sbjct: 124 SETIHLFSEQLVELDLMVRRMIMESFGI---EKYIDEHLNSTYYLTRLMKYTSPPDDDDD 183

Query: 187 EEVE-GLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWS 246
           EE + GL  HTD + +TI++Q Q  GL+VK+ + KW+ + P ++S  ++V +GD L A  
Sbjct: 184 EETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS--VLVMVGDSLCALL 243

Query: 247 NDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLK 304
           N R+ S  HRV++      R+S   F   +   ++ +PEELV++   R++KPF  +D+L 
Sbjct: 244 NGRLHSPYHRVIMTGKK-TRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 298

[Close]

BLAST of CSPI06G00480 vs. TrEMBL
Match: A0A0A0KB92_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G004480 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 2.6e-187
Identity = 322/323 (99.69%), Postives = 323/323 (100.00%), Query Frame = 1


Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240
           EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240

Query: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFR 300
           RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE+LVEEGGERMYKPFVCSDYLKFR
Sbjct: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300

Query: 301 ENDERGKFEKVGFTVTNFAGKKD 324
           ENDERGKFEKVGFTVTNFAGKKD
Sbjct: 301 ENDERGKFEKVGFTVTNFAGKKD 323

[Close]

BLAST of CSPI06G00480 vs. TrEMBL
Match: B9RDS1_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1615250 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 4.9e-130
Identity = 225/316 (71.20%), Postives = 279/316 (88.29%), Query Frame = 1


Query: 5   KASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPS 64
           + SVELPILDIS+P++ SSLSSLA+ACK+WGFF+I NHGISK++YNK++  S  IF+LPS
Sbjct: 5   QTSVELPILDISKPINQSSLSSLAEACKEWGFFNITNHGISKQLYNKIYLLSQNIFSLPS 64

Query: 65  ETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQ 124
           E K K+GP SS+ TYTPHFIASP+FE+LRVSGPNFLASAQ+SA+ LFNQ++S +FS++LQ
Sbjct: 65  EIKFKLGPSSSIKTYTPHFIASPYFESLRVSGPNFLASAQSSADVLFNQQNS-EFSDVLQ 124

Query: 125 EYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEEVEG 184
           EYG KMTELS++I++ +L+S G+G +KK++E  F NCHGYLRI NYTPP+ LED  EVEG
Sbjct: 125 EYGSKMTELSKRIVEMLLLSLGDGLDKKFYETEFNNCHGYLRIVNYTPPKNLED--EVEG 184

Query: 185 LGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRVRS 244
           LGMHTDMSC+TIVYQD+ GGLQVKS EGKW+DI+PCEE+  LVVNIGD+LQAWSN++ RS
Sbjct: 185 LGMHTDMSCITIVYQDEIGGLQVKSREGKWMDISPCEET--LVVNIGDMLQAWSNEKFRS 244

Query: 245 SEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFRENDE 304
           SEH+VVL++ A NR S+AFFW FED+KV+SAP+E+V EG  R+YKPFVCSDYLKFRE++E
Sbjct: 245 SEHQVVLKQSA-NRLSLAFFWCFEDQKVISAPDEVVGEGCGRIYKPFVCSDYLKFRESNE 304

Query: 305 RGKFEKVGFTVTNFAG 321
           RGKFEKVGFTV +FAG
Sbjct: 305 RGKFEKVGFTVKDFAG 314

[Close]

BLAST of CSPI06G00480 vs. TrEMBL
Match: B9GQ95_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s16090g PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 2.7e-128
Identity = 226/314 (71.97%), Postives = 268/314 (85.35%), Query Frame = 1


Query: 7   SVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSET 66
           S ELPILDISQPL PS+L+SLA+AC++WGFF I NHGISKE+YNKL+S S ++F LP+ET
Sbjct: 6   SAELPILDISQPLQPSALASLAEACQEWGFFRISNHGISKELYNKLYSLSQQLFGLPAET 65

Query: 67  KLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQEY 126
           KL++GP SS+NTYTPHFIASPFFE LRV GPNF ASAQ+SA+ LF Q+ S +F   LQEY
Sbjct: 66  KLELGPSSSINTYTPHFIASPFFECLRVFGPNFFASAQSSADTLFGQQ-SFEFRAALQEY 125

Query: 127 GDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEEVEGLG 186
           G KMTELS++I++ + MS GEGF+ KY+E  FKNCHGYLRI NY PP+ L D  EVEGLG
Sbjct: 126 GTKMTELSKRIVEILSMSLGEGFDNKYYESEFKNCHGYLRIINYNPPKGLVD--EVEGLG 185

Query: 187 MHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRVRSSE 246
           MHTDMSCVTIVYQD+ GGLQVKS EGKW+DI+P EE+  LVVNIGDLLQAWSND++RSSE
Sbjct: 186 MHTDMSCVTIVYQDEVGGLQVKSREGKWMDISPGEET--LVVNIGDLLQAWSNDKLRSSE 245

Query: 247 HRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFRENDERG 306
           HR+VL++P +NR S+AFFW FEDEKV+ AP E+V EG  R+Y+PFVCSDYLKFRE+ ERG
Sbjct: 246 HRIVLKKP-VNRLSLAFFWCFEDEKVIMAPNEVVGEGNARIYEPFVCSDYLKFRESSERG 305

Query: 307 KFEKVGFTVTNFAG 321
           KFEKVGFTV +FAG
Sbjct: 306 KFEKVGFTVKDFAG 313

[Close]

BLAST of CSPI06G00480 vs. TrEMBL
Match: W9RSL8_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_017281 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.6e-128
Identity = 231/321 (71.96%), Postives = 272/321 (84.74%), Query Frame = 1


Query: 1   MCVSKASVELPILDISQP-LSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEI 60
           M  S+ SV+L ILDIS+P L PSSLSSLA+ C+ WGFFHI NHGIS ++Y+K+ S S E+
Sbjct: 1   MSESQNSVKLHILDISEPVLHPSSLSSLAETCRTWGFFHITNHGISSDLYSKICSISKEL 60

Query: 61  FNLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQF 120
           FNLPSETKLK+GP SS  TYTPHFIASPFFE+LRVSGPNF ASAQNSA+ LF+Q+ S  F
Sbjct: 61  FNLPSETKLKLGPFSSTRTYTPHFIASPFFESLRVSGPNFFASAQNSADILFDQQCS-LF 120

Query: 121 SEILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDD 180
           SE+LQEYG KM ELS+KI++ +LMS G+GFE KY +  FKNCHGYLRINNY+PPE LED 
Sbjct: 121 SEMLQEYGSKMVELSKKIMETLLMSLGDGFETKY-KSEFKNCHGYLRINNYSPPENLEDH 180

Query: 181 EEVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSN 240
            EVEGLGMHTDMSCVTI+YQD+ GGLQV+S EGKW+DI+PCE +  L+VNIGD+LQAWSN
Sbjct: 181 GEVEGLGMHTDMSCVTIIYQDKMGGLQVRSEEGKWMDISPCEGT--LLVNIGDMLQAWSN 240

Query: 241 DRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKF 300
           +++RSSEHRVVLRRP  NRFS+AFF  FED+KVV AP+++V EG  R YKPFVC DYLKF
Sbjct: 241 EKLRSSEHRVVLRRPE-NRFSLAFFCCFEDDKVVLAPDDVVGEGNMRTYKPFVCLDYLKF 300

Query: 301 RENDERGKFEKVGFTVTNFAG 321
           REN+ERGKFEKV FTV +FAG
Sbjct: 301 RENNERGKFEKVKFTVKDFAG 316

[Close]

BLAST of CSPI06G00480 vs. TrEMBL
Match: M5X5L1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019644mg PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 2.5e-126
Identity = 224/322 (69.57%), Postives = 274/322 (85.09%), Query Frame = 1


Query: 3   VSKASVELPILDISQP-LSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFN 62
           + + SV+LP+LDISQP L PSSLSSLA+A K WGF HI NHGISK+++ KL+S SN +F+
Sbjct: 4   IPQTSVKLPMLDISQPILHPSSLSSLAEAFKTWGFLHITNHGISKDLFRKLYSLSNGLFS 63

Query: 63  LPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSE 122
           LPS+TKLK+GP SS  TYTPHFIASPFFE+LRVSGP F  SAQ SA  LF+Q +S +FSE
Sbjct: 64  LPSDTKLKLGPFSSAKTYTPHFIASPFFESLRVSGPKFFESAQTSANVLFDQHNS-EFSE 123

Query: 123 ILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLED-DE 182
           ILQEYG KMTELS+KI+K  LMS G+G  KK++E  F+NCHGYLRINNY+ PE+LED ++
Sbjct: 124 ILQEYGSKMTELSKKIVKIALMSLGDGLVKKFYESEFQNCHGYLRINNYSAPESLEDHED 183

Query: 183 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 242
           EVEGLGMHTDMSCVTIVYQD+ GGLQV+S EGKW+DI+PCE +  LVVNIGD+ QAWSN+
Sbjct: 184 EVEGLGMHTDMSCVTIVYQDEIGGLQVRSKEGKWMDISPCEGT--LVVNIGDMFQAWSNE 243

Query: 243 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFR 302
           ++RSSEHRV+L++P +NRFS+A+FW FEDEKV+ AP+++V E   R+YKPFVC DYLKFR
Sbjct: 244 KLRSSEHRVILKQP-VNRFSLAYFWCFEDEKVIFAPDDVVGEENVRIYKPFVCLDYLKFR 303

Query: 303 ENDERGKFEKVGFTVTNFAGKK 323
           E++ERGKFEKVGFTV +FAG K
Sbjct: 304 ESNERGKFEKVGFTVRDFAGIK 321

[Close]

BLAST of CSPI06G00480 vs. TAIR10
Match: AT4G23340.1 (AT4G23340.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 392.9 bits (1008), Expect = 1.9e-109
Identity = 190/319 (59.56%), Postives = 251/319 (78.68%), Query Frame = 1


Query: 6   ASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSE 65
           +S++LP+LD++QP+  S LSSL++ACK+WGFF++ NHGISKE+++K+ S S ++F  P E
Sbjct: 6   SSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKAPLE 65

Query: 66  TKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQE 125
           +KLK+GP+S    YTP +IASP+FE+L VSGP+F  SA+ SA+ LF      +  E +QE
Sbjct: 66  SKLKLGPIS----YTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELRETMQE 125

Query: 126 YGDKMTELSRKIIKNILM-SFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEE-VE 185
           YG KM ELS+++IK +LM + G+   K+ ++  F NCHGYLR+ NYTPP  +E  EE VE
Sbjct: 126 YGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELVE 185

Query: 186 GLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRVR 245
           GLGMHTDMSC+TIVYQD  GGLQ++S EGKW+DINPC  ++ LVVNIGDL+QAWSN R+R
Sbjct: 186 GLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC--NDFLVVNIGDLMQAWSNGRLR 245

Query: 246 SSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFREND 305
           SSEHRVVLR+  +NR S+AFF  FEDEKV+ AP+E+V EG +R YK F CS+YLKFR+++
Sbjct: 246 SSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSN 305

Query: 306 ERGKFEKVGFTVTNFAGKK 323
           E GKFEK+GFTV +FAG K
Sbjct: 306 EEGKFEKIGFTVKDFAGLK 317

[Close]

BLAST of CSPI06G00480 vs. TAIR10
Match: AT5G51310.1 (AT5G51310.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 367.5 bits (942), Expect = 8.7e-102
Identity = 185/328 (56.40%), Postives = 242/328 (73.78%), Query Frame = 1


Query: 7   SVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSET 66
           S+ELP+ DIS+PLS SSL+SL DACK+WGFF++ NHG+S+++Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQEY 126
           K+K+G     + YTP FIASPFFE+LRVSGP+F ASA++S +   +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEE----- 186
           G+KMT+L  KI+K IL SFG+    KY+E  F NCHGY RINNYT P   EDD       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 --VEGLGMHTDMSCVTIVYQDQTGGLQVKSSEG-KWVDINPCEESEALVVNIGDLLQAWS 246
             +EGLGMHTDMSC+TIV QD  GGLQV++ +G   +DINP  + EALVVN+GDLL AW+
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINP--KDEALVVNVGDLLHAWT 241

Query: 247 NDRVRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKVVSAPEELVEE-GGERMYKPFVCSD 306
           N R+RSS+HRV+L+R     NRFS+AFFW F+D KVV AP+E+V    G R+++ F C D
Sbjct: 242 NGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRVFRSFKCGD 301

Query: 307 YLKFRENDERGKFEKVGFTVTNFAGKKD 324
           YL+FRE++E+GKFEKVG TV +FA  +D
Sbjct: 302 YLRFRESNEKGKFEKVGDTVEDFARIED 323

[Close]

BLAST of CSPI06G00480 vs. TAIR10
Match: AT4G25420.1 (AT4G25420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 139.0 bits (349), Expect = 5.0e-33
Identity = 97/316 (30.70%), Postives = 167/316 (52.85%), Query Frame = 1


Query: 3   VSKASVELPILDISQPLS-PSSLSS----LADACKKWGFFHIKNHGISKEIYNKLHSFSN 62
           ++   +++P++D+   LS PSS       +++ACKK GFF + NHGIS+E+ +  H +++
Sbjct: 54  INVLELDVPLIDLQNLLSDPSSTLDASRLISEACKKHGFFLVVNHGISEELISDAHEYTS 113

Query: 63  EIFNLPSETKLKI-----GPLSSVNTYTPHFIAS-PFFETLRVSGPNFLASAQNSAEFLF 122
             F++P   K ++       +   +++T  F    P+ ETL     + ++ +++  ++  
Sbjct: 114 RFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFCDDMSRSKSVQDYFC 173

Query: 123 NQ--KSSHQFSEILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINN 182
           +        F ++ QEY + M+ LS KI++  L+    G ++ Y    F+     +R+N 
Sbjct: 174 DALGHGFQPFGKVYQEYCEAMSSLSLKIME--LLGLSLGVKRDYFREFFEENDSIMRLNY 233

Query: 183 YTPPETLEDDEEVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVN 242
           Y  P  ++ D  + G G H D + +TI++QD   GLQV   E +W  I P    +A VVN
Sbjct: 234 Y--PPCIKPDLTL-GTGPHCDPTSLTILHQDHVNGLQV-FVENQWRSIRP--NPKAFVVN 293

Query: 243 IGDLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYK 302
           IGD   A SNDR +S  HR V+   +  R S+AFF   + ++VV+ P EL++    R Y 
Sbjct: 294 IGDTFMALSNDRYKSCLHRAVVNSES-ERKSLAFFLCPKKDRVVTPPRELLDSITSRRYP 353

Query: 303 PFVCSDYLKFRENDER 306
            F  S +L+F +   R
Sbjct: 354 DFTWSMFLEFTQKHYR 360

[Close]

BLAST of CSPI06G00480 vs. TAIR10
Match: AT3G13610.1 (AT3G13610.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 137.5 bits (345), Expect = 1.5e-32
Identity = 95/308 (30.84%), Postives = 158/308 (51.30%), Query Frame = 1


Query: 3   VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNL 62
           V++    +P++D+S P       ++ DA +KWGFF + NHG+  E+ + + + +++ FNL
Sbjct: 55  VNETDEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 114

Query: 63  PSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAE-------FLFNQKS 122
           P E K K    +S++T            T+R  G +F   A+ + E       F  ++  
Sbjct: 115 PVEEKRKFTKENSLST------------TVRF-GTSFSPLAEQALEWKDYLSLFFVSEAE 174

Query: 123 SHQF-SEILQEYGDKMTELSRKIIKNILMSFGEGFE-KKYHEPHFKNCHGYLRIN-NYTP 182
           + QF  +I +    +    S+K+++ +L   G+    K+  E       G +R+N NY P
Sbjct: 175 AEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYP 234

Query: 183 PETLEDDEEVEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDINPCEESEALVVNIG 242
                + +   G+G H+D+S +TI+ QDQ GGL V+S + G WV + P   S   V+NIG
Sbjct: 235 --ICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGS--FVINIG 294

Query: 243 DLLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPF 300
           D +Q  SN   +S EHR VL     NR S+  F   + E V+    E++  G E +Y+  
Sbjct: 295 DAMQIMSNGLYKSVEHR-VLANGYNNRISVPIFVNPKPESVIGPLPEVIANGEEPIYRDV 344

[Close]

BLAST of CSPI06G00480 vs. TAIR10
Match: AT1G80330.1 (AT1G80330.1 gibberellin 3-oxidase 4)

HSP 1 Score: 137.5 bits (345), Expect = 1.5e-32
Identity = 101/301 (33.55%), Postives = 149/301 (49.50%), Query Frame = 1


Query: 10  LPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSETKLK 69
           +P++D+S   +P   + + DA K WG F I NHGIS+++ + + S S  +F++PSE KL+
Sbjct: 49  IPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERKLE 108

Query: 70  IGPL-SSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSH---QFSEILQE 129
                  V+ Y    I SPFFE    S       A +S    FN    H   ++  I+QE
Sbjct: 109 AASSDKGVSGYGEPRI-SPFFEKKMWS--EGFTIADDSYRNHFNTLWPHDHTKYCGIIQE 168

Query: 130 YGDKMTELSRKIIKNILMSFGEGFE-----KKYHEPHFKNCHGYLRINNYTPPETLEDDE 189
           Y D+M +L+ +++  IL S G   E      K  +   K   G +R+N+Y       + E
Sbjct: 169 YVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY---PVCPEPE 228

Query: 190 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 249
              GL  HTD + +TI++Q  TGGLQV   E  WV + P      LVVNIGDL    SN 
Sbjct: 229 RAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPA--PGVLVVNIGDLFHILSNG 288

Query: 250 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP-EELVEEGGERMYKPFVCSDYLKF 301
           ++ S  HR  +     +R SIA+ WG     V  AP  +L       +Y+     +YL+ 
Sbjct: 289 KIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEYLQI 337

[Close]

BLAST of CSPI06G00480 vs. NCBI nr
Match: gi|778709081|ref|XP_004138503.2| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 662.5 bits (1708), Expect = 3.7e-187
Identity = 322/323 (99.69%), Postives = 323/323 (100.00%), Query Frame = 1


Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240
           EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240

Query: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFR 300
           RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPE+LVEEGGERMYKPFVCSDYLKFR
Sbjct: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFVCSDYLKFR 300

Query: 301 ENDERGKFEKVGFTVTNFAGKKD 324
           ENDERGKFEKVGFTVTNFAGKKD
Sbjct: 301 ENDERGKFEKVGFTVTNFAGKKD 323

[Close]

BLAST of CSPI06G00480 vs. NCBI nr
Match: gi|659116735|ref|XP_008458231.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo])

HSP 1 Score: 632.9 bits (1631), Expect = 3.1e-178
Identity = 306/321 (95.33%), Postives = 316/321 (98.44%), Query Frame = 1


Query: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120
           +LP ETKLKIGPLSS+NTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180
           EILQEYG+KMTELSRKIIKN+L+SFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSND 240

Query: 241 RVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFR 300
           R+RSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAP+ELV EGG+RMYKPFVCSDYLKFR
Sbjct: 241 RLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFVCSDYLKFR 300

Query: 301 ENDERGKFEKVGFTVTNFAGK 322
           ENDERGKFEKVGFTV +FAGK
Sbjct: 301 ENDERGKFEKVGFTVRDFAGK 321

[Close]

BLAST of CSPI06G00480 vs. NCBI nr
Match: gi|255541592|ref|XP_002511860.1| (PREDICTED: gibberellin 20 oxidase 1 [Ricinus communis])

HSP 1 Score: 472.2 bits (1214), Expect = 7.1e-130
Identity = 225/316 (71.20%), Postives = 279/316 (88.29%), Query Frame = 1


Query: 5   KASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPS 64
           + SVELPILDIS+P++ SSLSSLA+ACK+WGFF+I NHGISK++YNK++  S  IF+LPS
Sbjct: 5   QTSVELPILDISKPINQSSLSSLAEACKEWGFFNITNHGISKQLYNKIYLLSQNIFSLPS 64

Query: 65  ETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQ 124
           E K K+GP SS+ TYTPHFIASP+FE+LRVSGPNFLASAQ+SA+ LFNQ++S +FS++LQ
Sbjct: 65  EIKFKLGPSSSIKTYTPHFIASPYFESLRVSGPNFLASAQSSADVLFNQQNS-EFSDVLQ 124

Query: 125 EYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEEVEG 184
           EYG KMTELS++I++ +L+S G+G +KK++E  F NCHGYLRI NYTPP+ LED  EVEG
Sbjct: 125 EYGSKMTELSKRIVEMLLLSLGDGLDKKFYETEFNNCHGYLRIVNYTPPKNLED--EVEG 184

Query: 185 LGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRVRS 244
           LGMHTDMSC+TIVYQD+ GGLQVKS EGKW+DI+PCEE+  LVVNIGD+LQAWSN++ RS
Sbjct: 185 LGMHTDMSCITIVYQDEIGGLQVKSREGKWMDISPCEET--LVVNIGDMLQAWSNEKFRS 244

Query: 245 SEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFRENDE 304
           SEH+VVL++ A NR S+AFFW FED+KV+SAP+E+V EG  R+YKPFVCSDYLKFRE++E
Sbjct: 245 SEHQVVLKQSA-NRLSLAFFWCFEDQKVISAPDEVVGEGCGRIYKPFVCSDYLKFRESNE 304

Query: 305 RGKFEKVGFTVTNFAG 321
           RGKFEKVGFTV +FAG
Sbjct: 305 RGKFEKVGFTVKDFAG 314

[Close]

BLAST of CSPI06G00480 vs. NCBI nr
Match: gi|694383856|ref|XP_009367858.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Pyrus x bretschneideri])

HSP 1 Score: 469.9 bits (1208), Expect = 3.5e-129
Identity = 231/323 (71.52%), Postives = 276/323 (85.45%), Query Frame = 1


Query: 3   VSKASVELPILDISQP-LSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFN 62
           + + SV+LP+LDISQP L PSSLSSLA+A K WGFFHI NHGISK+++ KL+S SN++F+
Sbjct: 4   IFQTSVKLPMLDISQPVLHPSSLSSLAEAFKTWGFFHITNHGISKDLFRKLYSLSNDLFS 63

Query: 63  LPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSE 122
           LPS TKLK+GP SS  TYTPHFIASP+FE+LRVSGPNF  SAQ+SA+ LF+Q +S +FSE
Sbjct: 64  LPSNTKLKLGPSSSAKTYTPHFIASPYFESLRVSGPNFFDSAQSSAQVLFDQHNS-EFSE 123

Query: 123 ILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNY-TPPETLEDD- 182
           ILQEYG KM ELS+KI+K  LMS G+G EKK +E  FKNCHGYLR+NNY TPPE+LED  
Sbjct: 124 ILQEYGSKMAELSKKIVKIALMSLGDGLEKKQYETEFKNCHGYLRVNNYSTPPESLEDQH 183

Query: 183 EEVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSN 242
           +EVEGLGMHTDMSCVTIVYQ++ GGLQV+S EGKW+DI PCE +  LVVNIGD+ QAWSN
Sbjct: 184 QEVEGLGMHTDMSCVTIVYQEEIGGLQVRSKEGKWMDITPCEGT--LVVNIGDMFQAWSN 243

Query: 243 DRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKF 302
           D +RSSEHRV+L++P  NRFS+AFFW FEDEKV+ APE++V EG ER+YKPFVC DYLKF
Sbjct: 244 DNLRSSEHRVILKQPK-NRFSLAFFWSFEDEKVIFAPEDVVGEGNERIYKPFVCLDYLKF 303

Query: 303 RENDERGKFEKVGFTVTNFAGKK 323
           RE++ERGKFEKVGFTV +FAG K
Sbjct: 304 RESNERGKFEKVGFTVRDFAGIK 322

[Close]

BLAST of CSPI06G00480 vs. NCBI nr
Match: gi|224067894|ref|XP_002302586.1| (hypothetical protein POPTR_0002s16090g [Populus trichocarpa])

HSP 1 Score: 466.5 bits (1199), Expect = 3.9e-128
Identity = 226/314 (71.97%), Postives = 268/314 (85.35%), Query Frame = 1


Query: 7   SVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIFNLPSET 66
           S ELPILDISQPL PS+L+SLA+AC++WGFF I NHGISKE+YNKL+S S ++F LP+ET
Sbjct: 6   SAELPILDISQPLQPSALASLAEACQEWGFFRISNHGISKELYNKLYSLSQQLFGLPAET 65

Query: 67  KLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFSEILQEY 126
           KL++GP SS+NTYTPHFIASPFFE LRV GPNF ASAQ+SA+ LF Q+ S +F   LQEY
Sbjct: 66  KLELGPSSSINTYTPHFIASPFFECLRVFGPNFFASAQSSADTLFGQQ-SFEFRAALQEY 125

Query: 127 GDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDEEVEGLG 186
           G KMTELS++I++ + MS GEGF+ KY+E  FKNCHGYLRI NY PP+ L D  EVEGLG
Sbjct: 126 GTKMTELSKRIVEILSMSLGEGFDNKYYESEFKNCHGYLRIINYNPPKGLVD--EVEGLG 185

Query: 187 MHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCEESEALVVNIGDLLQAWSNDRVRSSE 246
           MHTDMSCVTIVYQD+ GGLQVKS EGKW+DI+P EE+  LVVNIGDLLQAWSND++RSSE
Sbjct: 186 MHTDMSCVTIVYQDEVGGLQVKSREGKWMDISPGEET--LVVNIGDLLQAWSNDKLRSSE 245

Query: 247 HRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVEEGGERMYKPFVCSDYLKFRENDERG 306
           HR+VL++P +NR S+AFFW FEDEKV+ AP E+V EG  R+Y+PFVCSDYLKFRE+ ERG
Sbjct: 246 HRIVLKKP-VNRLSLAFFWCFEDEKVIMAPNEVVGEGNARIYEPFVCSDYLKFRESSERG 305

Query: 307 KFEKVGFTVTNFAG 321
           KFEKVGFTV +FAG
Sbjct: 306 KFEKVGFTVKDFAG 313

The following BLAST results are available for this feature:

BLAST of CSPI06G00480 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GAOX1_ARATH	8.9e-32	30.70	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2	[more]
F6H1_ARATH	2.6e-31	30.84	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana GN=F6'H1 PE=1 SV=1	[more]
G3OX4_ARATH	2.6e-31	33.55	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana GN=GA3OX4 PE=1 SV=1	[more]
F6H2_ARATH	5.8e-31	32.11	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana GN=F6'H2 PE=1 SV=1	[more]
AOP1V_ARATH	1.7e-30	28.85	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]

BLAST of CSPI06G00480 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KB92_CUCSA	2.6e-187	99.69	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G004480 PE=3 SV=1	[more]
B9RDS1_RICCO	4.9e-130	71.20	Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1615250 PE=3 SV=1	[more]
B9GQ95_POPTR	2.7e-128	71.97	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s16090g PE=3 SV=1	[more]
W9RSL8_9ROSA	4.6e-128	71.96	Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_017281 PE=3 SV=1	[more]
M5X5L1_PRUPE	2.5e-126	69.57	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019644mg PE=3 SV=1	[more]

BLAST of CSPI06G00480 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G23340.1	1.9e-109	59.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G51310.1	8.7e-102	56.40	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT4G25420.1	5.0e-33	30.70	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT3G13610.1	1.5e-32	30.84	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G80330.1	1.5e-32	33.55	gibberellin 3-oxidase 4	[more]

BLAST of CSPI06G00480 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778709081\|ref\|XP_004138503.2\|	3.7e-187	99.69	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus]	[more]
gi\|659116735\|ref\|XP_008458231.1\|	3.1e-178	95.33	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo]	[more]
gi\|255541592\|ref\|XP_002511860.1\|	7.1e-130	71.20	PREDICTED: gibberellin 20 oxidase 1 [Ricinus communis]	[more]
gi\|694383856\|ref\|XP_009367858.1\|	3.5e-129	71.52	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Pyrus x bretschne...	[more]
gi\|224067894\|ref\|XP_002302586.1\|	3.9e-128	71.97	hypothetical protein POPTR_0002s16090g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0047927	gibberellin-44 dioxygenase activity
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G00480.1	CSPI06G00480.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 166..264 score: 4.1
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 161..267 score: 11
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..76 score: 2.0
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 7..305 score: 8.5
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..309 score: 2.1E
None	No IPR available	PANTHER	PTHR10209:SF151	SUBFAMILY NOT NAMED	coord: 1..309 score: 2.1E
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 7..303 score: 1.51

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI06G00480	Cla001227	Watermelon (97103) v1	cpiwmB558
CSPI06G00480	Csa6G004480	Cucumber (Chinese Long) v2	cpicuB314
CSPI06G00480	MELO3C021211	Melon (DHL92) v3.5.1	cpimeB418
CSPI06G00480	ClCG06G003700	Watermelon (Charleston Gray)	cpiwcgB507
CSPI06G00480	Lsi09G015640	Bottle gourd (USVL1VR-Ls)	cpilsiB397
CSPI06G00480	MELO3C021211.2	Melon (DHL92) v3.6.1	cpimedB413
CSPI06G00480	CsaV3_6G000540	Cucumber (Chinese Long) v3	cpicucB364
CSPI06G00480	Cla97C06G113150	Watermelon (97103) v2	cpiwmbB499
CSPI06G00480	Bhi12G001655	Wax gourd	cpiwgoB538
CSPI06G00480	Cucsa.135520	Cucumber (Gy14) v1	cgycpiB198
CSPI06G00480	CmaCh17G004320	Cucurbita maxima (Rimu)	cmacpiB372
CSPI06G00480	CmoCh17G004110	Cucurbita moschata (Rifu)	cmocpiB360
CSPI06G00480	Cp4.1LG12g03730	Cucurbita pepo (Zucchini)	cpecpiB143
CSPI06G00480	CsGy6G000540	Cucumber (Gy14) v2	cgybcpiB288
CSPI06G00480	Carg05603	Silver-seed gourd	carcpiB0077

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI06G00480	Cucumber (Chinese Long) v3	cpicucB359
CSPI06G00480	Watermelon (97103) v2	cpiwmbB435
CSPI06G00480	Wax gourd	cpiwgoB546
CSPI06G00480	Wild cucumber (PI 183967)	cpicpiB182
CSPI06G00480	Cucumber (Gy14) v1	cgycpiB458
CSPI06G00480	Cucurbita maxima (Rimu)	cmacpiB319
CSPI06G00480	Cucurbita maxima (Rimu)	cmacpiB647
CSPI06G00480	Cucurbita maxima (Rimu)	cmacpiB925
CSPI06G00480	Cucurbita moschata (Rifu)	cmocpiB310
CSPI06G00480	Cucurbita moschata (Rifu)	cmocpiB641
CSPI06G00480	Cucurbita moschata (Rifu)	cmocpiB903
CSPI06G00480	Cucumber (Chinese Long) v2	cpicuB309
CSPI06G00480	Melon (DHL92) v3.5.1	cpimeB412
CSPI06G00480	Watermelon (Charleston Gray)	cpiwcgB455
CSPI06G00480	Watermelon (97103) v1	cpiwmB523
CSPI06G00480	Cucurbita pepo (Zucchini)	cpecpiB317
CSPI06G00480	Bottle gourd (USVL1VR-Ls)	cpilsiB408
CSPI06G00480	Melon (DHL92) v3.6.1	cpimedB408
CSPI06G00480	Cucumber (Gy14) v2	cgybcpiB243
CSPI06G00480	Silver-seed gourd	carcpiB0966