Carg03860 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg03860
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	calmodulin-like protein 7
Location	Cucurbita_argyrosperma_scaffold_011 : 489058 .. 489594 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATCTCCAGATTCATTGGGGACTTTTTACAAACAGTTAGTGGGCGATCCCGTGCGGCGGATTCCACCGCTGACGGCGGTGCTCCGAGTCCCCACAGAGTCATCCACGACGACGTGCTACTACGGGCGTTGATTACAGTGTTTGGAATGAGGGAAAATGGGAGAATCAAGAAGGAAAAAGCAAAAGGGGTGGTGGAGAAATTGGGGCTTATAAAGAGTGAAGAAGAGAAGGAAGGGTTTGAGCTGCCGGCGGATGGCGGTGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGATGGAGGAGGAAGGGAAACGAAATGAGCTGCTATGGGAAGCTTTTAAGATATTTGATGTGGATGGCGATGGATTCATTGATACGGTTGAGCTGAAAAGAGTGCTTGAATGTTTGGGATTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGCGTTGTGGATGTGAATTTGGATGGGAAAGTTGATTTTGGTGAGTTTGAATTAATGATGGGTGTGAAATGTTCTTGA

mRNA sequence

ATGGGTATCTCCAGATTCATTGGGGACTTTTTACAAACAGTTAGTGGGCGATCCCGTGCGGCGGATTCCACCGCTGACGGCGGTGCTCCGAGTCCCCACAGAGTCATCCACGACGACGTGCTACTACGGGCGTTGATTACAGTGTTTGGAATGAGGGAAAATGGGAGAATCAAGAAGGAAAAAGCAAAAGGGGTGGTGGAGAAATTGGGGCTTATAAAGAGTGAAGAAGAGAAGGAAGGGTTTGAGCTGCCGGCGGATGGCGGTGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGATGGAGGAGGAAGGGAAACGAAATGAGCTGCTATGGGAAGCTTTTAAGATATTTGATGTGGATGGCGATGGATTCATTGATACGGTTGAGCTGAAAAGAGTGCTTGAATGTTTGGGATTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGCGTTGTGGATGTGAATTTGGATGGGAAAGTTGATTTTGGTGAGTTTGAATTAATGATGGGTGTGAAATGTTCTTGA

Coding sequence (CDS)

ATGGGTATCTCCAGATTCATTGGGGACTTTTTACAAACAGTTAGTGGGCGATCCCGTGCGGCGGATTCCACCGCTGACGGCGGTGCTCCGAGTCCCCACAGAGTCATCCACGACGACGTGCTACTACGGGCGTTGATTACAGTGTTTGGAATGAGGGAAAATGGGAGAATCAAGAAGGAAAAAGCAAAAGGGGTGGTGGAGAAATTGGGGCTTATAAAGAGTGAAGAAGAGAAGGAAGGGTTTGAGCTGCCGGCGGATGGCGGTGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGATGGAGGAGGAAGGGAAACGAAATGAGCTGCTATGGGAAGCTTTTAAGATATTTGATGTGGATGGCGATGGATTCATTGATACGGTTGAGCTGAAAAGAGTGCTTGAATGTTTGGGATTGGATAAAGGGTGGGGGATTAGGGAGATTGAGAAAATGGTTAGCGTTGTGGATGTGAATTTGGATGGGAAAGTTGATTTTGGTGAGTTTGAATTAATGATGGGTGTGAAATGTTCTTGA

Protein sequence

MGISRFIGDFLQTVSGRSRAADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS

Homology

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BLAST of Carg03860 vs. NCBI nr
Match: XP_022951438.1 (calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 351.7 bits (901), Expect = 1.5e-93
Identity = 177/178 (99.44%), Postives = 177/178 (99.44%), Query Frame = 0


Query: 1   MGISRFIGDFLQTVSGRSRAADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKKE 60
           MGISRFIGDFLQTV GRSRAADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKKE
Sbjct: 1   MGISRFIGDFLQTVGGRSRAADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKKE 60

Query: 61  KAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFDV 120
           KAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFDV
Sbjct: 61  KAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFDV 120

Query: 121 DGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS 179
           DGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS
Sbjct: 121 DGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS 178

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BLAST of Carg03860 vs. NCBI nr
Match: XP_023002517.1 (calmodulin-like protein 2 [Cucurbita maxima])

HSP 1 Score: 345.5 bits (885), Expect = 1.1e-91
Identity = 175/178 (98.31%), Postives = 175/178 (98.31%), Query Frame = 0


Query: 1   MGISRFIGDFLQTVSGRSRAADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKKE 60
           MGISRFIGDFLQTV GRSRAADS ADGGAPSP RVIHDDVLLRALITVFGMRENGRIKKE
Sbjct: 1   MGISRFIGDFLQTVGGRSRAADSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKKE 60

Query: 61  KAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFDV 120
           KAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFDV
Sbjct: 61  KAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFDV 120

Query: 121 DGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS 179
           DGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS
Sbjct: 121 DGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKCS 178

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BLAST of Carg03860 vs. NCBI nr
Match: XP_008445056.1 (PREDICTED: calmodulin-like protein 7 [Cucumis melo])

HSP 1 Score: 283.9 bits (725), Expect = 3.8e-73
Identity = 150/178 (84.27%), Postives = 160/178 (89.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTVSGRSR-AADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+ GRSR AA+ST D G   P+R+++DDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120
           EKAKGVVEKLGLI   EEKE FEL A  GGDEVAVEEVIG  EEEGKRNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIG--EEEGKRNELLYEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 178
           VDGDGFIDT+ELKRV++CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 VDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 173

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BLAST of Carg03860 vs. NCBI nr
Match: XP_004138884.1 (PREDICTED: calmodulin-like protein 2 [Cucumis sativus] >KGN62844.1 hypothetical protein Csa_2G376820 [Cucumis sativus])

HSP 1 Score: 282.7 bits (722), Expect = 8.5e-73
Identity = 149/178 (83.71%), Postives = 160/178 (89.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTVSGRSRAA-DSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+ GRSRAA +ST D G   P+R+++DDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAVESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120
           EKAKGVVEKLGLI   EEKE FEL A  GGDEVAVEE+IG  EEEGKRNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEMIG--EEEGKRNELLYEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 178
           VDGDGFIDT+ELKRV++CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 VDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 173

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BLAST of Carg03860 vs. NCBI nr
Match: XP_022132261.1 (calmodulin-like protein 2 [Momordica charantia])

HSP 1 Score: 267.3 bits (682), Expect = 3.7e-68
Identity = 143/181 (79.01%), Postives = 151/181 (83.43%), Query Frame = 0


Query: 1   MGISRFIGDFLQTVSGRSR-AADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+ G  R AA S A      P    +DD LL ALITVFGMRENGRIKK
Sbjct: 72  MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 131

Query: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFK 120
           EKAKGVVEKLGLI+ EEEK  F+LP   GGDEVAVEEVIGEM EE   GK++ELL EAFK
Sbjct: 132 EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 191

Query: 121 IFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVK 178
           IFDVDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMV VVDVNLDGKVDFGEF+LMMGVK
Sbjct: 192 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 251

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BLAST of Carg03860 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 78.2 bits (191), Expect = 5.8e-15
Identity = 49/136 (36.03%), Postives = 74/136 (54.41%), Query Frame = 0


Query: 42  LRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIG-- 101
           L  +  +F    +G+I K + K   + +G++  E E        D  GD     +  G  
Sbjct: 6   LSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSL 65

Query: 102 --EMEEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVD 161
             EM EE +  E + EAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VD
Sbjct: 66  YQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 125

Query: 162 VNLDGKVDFGEFELMM 174
           V+ DG V+F EF+ MM
Sbjct: 126 VDGDGMVNFKEFKQMM 141

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BLAST of Carg03860 vs. TAIR10
Match: AT5G44460.1 (calmodulin like 43)

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-13
Identity = 37/70 (52.86%), Postives = 48/70 (68.57%), Query Frame = 0


Query: 104 EGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGK 163
           +G     L EAF +FD DGDGFI  VEL++VL+ LGL +   I ++EKM+  VD N DG+
Sbjct: 105 DGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGR 164

Query: 164 VDFGEFELMM 174
           VDF EF+ MM
Sbjct: 165 VDFFEFKNMM 174

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BLAST of Carg03860 vs. TAIR10
Match: AT4G20780.1 (calmodulin like 42)

HSP 1 Score: 69.7 bits (169), Expect = 2.1e-12
Identity = 35/63 (55.56%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 111 LWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFE 170
           L EAFK+FD +GDGFI   EL+ VL+ LGL +G  +  +EKM+  VD N DG+VDF EF+
Sbjct: 121 LAEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFK 180

Query: 171 LMM 174
            MM
Sbjct: 181 NMM 183

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BLAST of Carg03860 vs. TAIR10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 65.1 bits (157), Expect = 5.1e-11
Identity = 40/117 (34.19%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 57  IKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFK 116
           I +E+A+ V+  LGL  ++++ +          ++ + +EV    EE+    E + +AF 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQ----------EQYSAKEVSSLFEEKEASLEEVKQAFD 142

Query: 117 IFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMM 174
           +FD + DGFID +EL+RVL  LG  +G  +     M+  +D N DGK+DF EF   M
Sbjct: 143 VFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFM 189

[Close]

BLAST of Carg03860 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 62.8 bits (151), Expect = 2.5e-10
Identity = 46/136 (33.82%), Postives = 67/136 (49.26%), Query Frame = 0


Query: 42  LRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEM 101
           L A+   F +  +G+I  ++   ++  LG    EEE E      D  GD     E   E+
Sbjct: 38  LEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVEL 97

Query: 102 EEEGKRN----ELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVD 161
             +G       E L +AF ++D+DG+G I   EL  VL  LG +    I E  KM+  VD
Sbjct: 98  NTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE--CSIAECRKMIGGVD 157

Query: 162 VNLDGKVDFGEFELMM 174
            + DG +DF EF++MM
Sbjct: 158 KDGDGTIDFEEFKIMM 171

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BLAST of Carg03860 vs. Swiss-Prot
Match: sp|Q9SU00|CML2_ARATH (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.0e-13
Identity = 49/136 (36.03%), Postives = 74/136 (54.41%), Query Frame = 0


Query: 42  LRALITVFGMRENGRIKKEKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIG-- 101
           L  +  +F    +G+I K + K   + +G++  E E        D  GD     +  G  
Sbjct: 6   LSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSL 65

Query: 102 --EMEEEGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVD 161
             EM EE +  E + EAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VD
Sbjct: 66  YQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 125

Query: 162 VNLDGKVDFGEFELMM 174
           V+ DG V+F EF+ MM
Sbjct: 126 VDGDGMVNFKEFKQMM 141

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BLAST of Carg03860 vs. Swiss-Prot
Match: sp|Q10LX4|CML27_ORYSJ (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 GN=CML27 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 53/151 (35.10%), Postives = 78/151 (51.66%), Query Frame = 0


Query: 42  LRALITVFGMRENGRIKKEKAKGVVEKLGLIKSE---EEKEGFELPADGG----GDEVAV 101
           LR +  +F    +G I  ++    ++ LGL       E   G  +PA       GD  A+
Sbjct: 32  LRRVFDLFDRNGDGEITLDEMASALDALGLGADRAGLEATVGGYIPAGAAGLRFGDFEAL 91

Query: 102 EEVIG-------EMEEEGKRNE-----LLWEAFKIFDVDGDGFIDTVELKRVLECLGLDK 161
              +G       E EE GK+ E      + EAF++FD DGDGFI   EL+ VL+ LGL +
Sbjct: 92  HRALGDALFGPVEEEEPGKQGEDDDEGDMKEAFRVFDEDGDGFISAAELQAVLKKLGLPE 151

Query: 162 GWGIREIEKMVSVVDVNLDGKVDFGEFELMM 174
              +  +++M+  VD + DG+VDFGEF+ MM
Sbjct: 152 ARNLATVQEMICNVDRDCDGRVDFGEFKCMM 182

[Close]

BLAST of Carg03860 vs. Swiss-Prot
Match: sp|Q9FI19|CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-12
Identity = 37/70 (52.86%), Postives = 48/70 (68.57%), Query Frame = 0


Query: 104 EGKRNELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGK 163
           +G     L EAF +FD DGDGFI  VEL++VL+ LGL +   I ++EKM+  VD N DG+
Sbjct: 105 DGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGR 164

Query: 164 VDFGEFELMM 174
           VDF EF+ MM
Sbjct: 165 VDFFEFKNMM 174

[Close]

BLAST of Carg03860 vs. Swiss-Prot
Match: sp|Q84UL5|CML32_ORYSJ (Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 GN=CML32 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.7e-12
Identity = 34/61 (55.74%), Postives = 44/61 (72.13%), Query Frame = 0


Query: 113 EAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELM 172
           EAFK+FDVDGDGFI   EL+ VL+ LGL +   +  + +M+  VD N DG+VDFGEF+ M
Sbjct: 128 EAFKVFDVDGDGFISASELQEVLKKLGLPEAGSLATVREMICNVDRNSDGRVDFGEFKSM 187

Query: 173 M 174
           M
Sbjct: 188 M 188

[Close]

BLAST of Carg03860 vs. Swiss-Prot
Match: sp|Q7XHW4|CML24_ORYSJ (Probable calcium-binding protein CML24 OS=Oryza sativa subsp. japonica OX=39947 GN=CML24 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-11
Identity = 32/61 (52.46%), Postives = 44/61 (72.13%), Query Frame = 0


Query: 113 EAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELM 172
           EAFK+FDVDGDGFI   EL+ VL+ LG+ +   +  + +M+  VD + DG+VDFGEF+ M
Sbjct: 129 EAFKVFDVDGDGFISASELQEVLKKLGMPEAGSLANVREMICNVDRDSDGRVDFGEFKCM 188

Query: 173 M 174
           M
Sbjct: 189 M 189

[Close]

BLAST of Carg03860 vs. TrEMBL
Match: tr|A0A1S3BBS0|A0A1S3BBS0_CUCME (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103488209 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 2.5e-73
Identity = 150/178 (84.27%), Postives = 160/178 (89.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTVSGRSR-AADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+ GRSR AA+ST D G   P+R+++DDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120
           EKAKGVVEKLGLI   EEKE FEL A  GGDEVAVEEVIG  EEEGKRNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIG--EEEGKRNELLYEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 178
           VDGDGFIDT+ELKRV++CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 VDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 173

[Close]

BLAST of Carg03860 vs. TrEMBL
Match: tr|A0A0A0LP90|A0A0A0LP90_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G376820 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 5.6e-73
Identity = 149/178 (83.71%), Postives = 160/178 (89.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTVSGRSRAA-DSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+ GRSRAA +ST D G   P+R+++DDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAVESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120
           EKAKGVVEKLGLI   EEKE FEL A  GGDEVAVEE+IG  EEEGKRNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEMIG--EEEGKRNELLYEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 178
           VDGDGFIDT+ELKRV++CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 VDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 173

[Close]

BLAST of Carg03860 vs. TrEMBL
Match: tr|A0A2N9JA27|A0A2N9JA27_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS61285 PE=4 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.1e-47
Identity = 112/179 (62.57%), Postives = 126/179 (70.39%), Query Frame = 0


Query: 4   SRFIGDFLQTVSGRSRAADSTADGGAPS--PHRVIH-DDVLLRALITVFGMRENGRIKKE 63
           SR IGD L   +     + +  D   P+  PH     DD ++RALITVFGM  NGRIKKE
Sbjct: 8   SRLIGDLLHAFANSKPGSAAHLDKDQPTCKPHNACKVDDTMVRALITVFGMENNGRIKKE 67

Query: 64  KAKGVVEKLGLIKSEEE--KEGFELPADG----GGDEVAVEEVIGEMEEEGKRNELLWEA 123
            A+ VVEKLGLI S EE  K  FELP  G    G DEV VEEV+G +E+  KRNELL EA
Sbjct: 68  NARRVVEKLGLIYSSEEDNKPSFELPNQGGLGLGDDEVPVEEVLGGLEDVSKRNELLQEA 127

Query: 124 FKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMM 174
           FKIFD DGDGFI+ VELKRVLECLGLD GW + EI+KMV  VD+NLDGKVDFGEFELMM
Sbjct: 128 FKIFDEDGDGFIEAVELKRVLECLGLDNGWDMGEIQKMVKAVDLNLDGKVDFGEFELMM 186

[Close]

BLAST of Carg03860 vs. TrEMBL
Match: tr|M5Y644|M5Y644_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G455200 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.4e-47
Identity = 111/179 (62.01%), Postives = 125/179 (69.83%), Query Frame = 0


Query: 4   SRFIGDFLQTVSGRSRAAD--STADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKKEK 63
           SR +GD +Q   G SR +      +G          D+ + RALITVFGM  NGRIKKE 
Sbjct: 8   SRLLGDLVQAFGGVSRPSTECKPQNGXXXXXXXXXVDESMARALITVFGMETNGRIKKEN 67

Query: 64  AKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFKIF 123
           A+ VVE LGLI  EE+K  F+LP D    EV VEEV+G +EEE    KRNELL EAFKIF
Sbjct: 68  ARRVVENLGLICHEEDKSSFDLPGD--DHEVPVEEVLGGLEEEDDGSKRNELLLEAFKIF 127

Query: 124 DVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 178
           D DGDGFI+ VELKRVLECLGL  GW + +IEKMV VVDVNLDGKVDF EFELMMGVKC
Sbjct: 128 DEDGDGFIEAVELKRVLECLGLGSGWDMDQIEKMVRVVDVNLDGKVDFNEFELMMGVKC 184

[Close]

BLAST of Carg03860 vs. TrEMBL
Match: tr|A0A2P4I020|A0A2P4I020_QUESU (Putative calcium-binding protein cml43 OS=Quercus suber OX=58331 GN=CFP56_79139 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.9e-45
Identity = 113/186 (60.75%), Postives = 126/186 (67.74%), Query Frame = 0


Query: 1   MGIS----RFIGDFLQTV--SGRSRAADSTADGGAPSPHRVIH-DDVLLRALITVFGMRE 60
           MGI+    R IGD L     S  S AA    D     P      DD ++RALITVFGM  
Sbjct: 1   MGITGVCIRLIGDMLHAFGNSKPSSAAHLDEDHSTCKPQNACKVDDTMVRALITVFGMEN 60

Query: 61  NGRIKKEKAKGVVEKLGL-IKSEEEKEGFELPADGGG-----DEVAVEEVIGEMEEEGKR 120
           NGRIKKE A+ VVEKLGL   SEE+K  FELP D  G     DEV VEEV+G +E+  KR
Sbjct: 61  NGRIKKENARRVVEKLGLTYSSEEDKSSFELPGDQDGVGLEDDEVPVEEVLGGLEDMSKR 120

Query: 121 NELLWEAFKIFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFG 174
           NELL EAFKIFD DG+GFI+ VELKRVL+CLGLD GW + EIEKMV  VD+N DGKVDFG
Sbjct: 121 NELLHEAFKIFDEDGNGFIEAVELKRVLDCLGLDCGWDMGEIEKMVKAVDLNFDGKVDFG 180

The following BLAST results are available for this feature:

BLAST of Carg03860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951438.1	1.5e-93	99.44	calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like p...	[more]
XP_023002517.1	1.1e-91	98.31	calmodulin-like protein 2 [Cucurbita maxima]	[more]
XP_008445056.1	3.8e-73	84.27	PREDICTED: calmodulin-like protein 7 [Cucumis melo]	[more]
XP_004138884.1	8.5e-73	83.71	PREDICTED: calmodulin-like protein 2 [Cucumis sativus] >KGN62844.1 hypothetical ...	[more]
XP_022132261.1	3.7e-68	79.01	calmodulin-like protein 2 [Momordica charantia]	[more]

BLAST of Carg03860 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G12860.1	5.8e-15	36.03	EF hand calcium-binding protein family	[more]
AT5G44460.1	1.4e-13	52.86	calmodulin like 43	[more]
AT4G20780.1	2.1e-12	55.56	calmodulin like 42	[more]
AT3G29000.1	5.1e-11	34.19	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	2.5e-10	33.82	EF hand calcium-binding protein family	[more]

BLAST of Carg03860 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9SU00\|CML2_ARATH	1.0e-13	36.03	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
sp\|Q10LX4\|CML27_ORYSJ	1.2e-12	35.10	Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q9FI19\|CML43_ARATH	2.6e-12	52.86	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
sp\|Q84UL5\|CML32_ORYSJ	5.7e-12	55.74	Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
sp\|Q7XHW4\|CML24_ORYSJ	2.2e-11	52.46	Probable calcium-binding protein CML24 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Carg03860 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BBS0\|A0A1S3BBS0_CUCME	2.5e-73	84.27	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103488209 PE=4 SV=1	[more]
tr\|A0A0A0LP90\|A0A0A0LP90_CUCSA	5.6e-73	83.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G376820 PE=4 SV=1	[more]
tr\|A0A2N9JA27\|A0A2N9JA27_FAGSY	1.1e-47	62.57	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS61285 PE=4 SV=1	[more]
tr\|M5Y644\|M5Y644_PRUPE	2.4e-47	62.01	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G455200 PE=4 SV=1	[more]
tr\|A0A2P4I020\|A0A2P4I020_QUESU	2.9e-45	60.75	Putative calcium-binding protein cml43 OS=Quercus suber OX=58331 GN=CFP56_79139 ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03860-RA	Carg03860-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 110..138 e-value: 0.0074 score: 25.5 coord: 148..176 e-value: 1.1 score: 17.4
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 111..139 e-value: 6.1E-8 score: 31.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 144..178 score: 8.851
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 106..141 score: 14.82
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 113..169 e-value: 1.97586E-13 score: 61.0245
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 9..176 e-value: 7.9E-20 score: 73.6
None	No IPR available	PANTHER	PTHR45003:SF2	CALCIUM-BINDING PROTEIN CML42-RELATED	coord: 100..173
None	No IPR available	PANTHER	PTHR45003	FAMILY NOT NAMED	coord: 100..173
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 119..131
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 48..173

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg03860	Cla022233	Watermelon (97103) v1	carwmB0978
Carg03860	Csa2G376820	Cucumber (Chinese Long) v2	carcuB0987
Carg03860	MELO3C011166	Melon (DHL92) v3.5.1	carmeB0955
Carg03860	ClCG08G012470	Watermelon (Charleston Gray)	carwcgB0958
Carg03860	CSPI02G22530	Wild cucumber (PI 183967)	carcpiB1010
Carg03860	CmaCh12G002700	Cucurbita maxima (Rimu)	carcmaB1349
Carg03860	CmoCh12G002230	Cucurbita moschata (Rifu)	carcmoB1310
Carg03860	Lsi08G011080	Bottle gourd (USVL1VR-Ls)	carlsiB898
Carg03860	Cp4.1LG07g02180	Cucurbita pepo (Zucchini)	carcpeB1368
Carg03860	CsGy2G022300	Cucumber (Gy14) v2	carcgybB0960
Carg03860	MELO3C011166.2	Melon (DHL92) v3.6.1	carmedB0961
Carg03860	CsaV3_2G031170	Cucumber (Chinese Long) v3	carcucB1015
Carg03860	Cla97C08G155670	Watermelon (97103) v2	carwmbB1034
Carg03860	Bhi04G000897	Wax gourd	carwgoB1145

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg03860	Silver-seed gourd	carcarB071
Carg03860	Silver-seed gourd	carcarB072
Carg03860	Silver-seed gourd	carcarB513
Carg03860	Silver-seed gourd	carcarB558
Carg03860	Cucumber (Gy14) v2	carcgybB0963
Carg03860	Cucumber (Gy14) v2	carcgybB0965
Carg03860	Cucumber (Gy14) v1	carcgyB1091
Carg03860	Cucumber (Gy14) v1	carcgyB1093
Carg03860	Cucumber (Gy14) v1	carcgyB1097
Carg03860	Cucurbita maxima (Rimu)	carcmaB1348
Carg03860	Cucurbita maxima (Rimu)	carcmaB1356
Carg03860	Cucurbita maxima (Rimu)	carcmaB1358
Carg03860	Cucurbita moschata (Rifu)	carcmoB1308
Carg03860	Cucurbita moschata (Rifu)	carcmoB1315
Carg03860	Cucurbita moschata (Rifu)	carcmoB1318
Carg03860	Cucurbita moschata (Rifu)	carcmoB1320
Carg03860	Cucurbita pepo (Zucchini)	carcpeB1351
Carg03860	Cucurbita pepo (Zucchini)	carcpeB1353
Carg03860	Cucurbita pepo (Zucchini)	carcpeB1363
Carg03860	Cucurbita pepo (Zucchini)	carcpeB1366
Carg03860	Wild cucumber (PI 183967)	carcpiB1014
Carg03860	Wild cucumber (PI 183967)	carcpiB1016
Carg03860	Cucumber (Chinese Long) v2	carcuB0990
Carg03860	Cucumber (Chinese Long) v2	carcuB0993
Carg03860	Cucumber (Chinese Long) v3	carcucB1018
Carg03860	Cucumber (Chinese Long) v3	carcucB1022
Carg03860	Bottle gourd (USVL1VR-Ls)	carlsiB897
Carg03860	Bottle gourd (USVL1VR-Ls)	carlsiB899
Carg03860	Melon (DHL92) v3.5.1	carmeB0954
Carg03860	Melon (DHL92) v3.5.1	carmeB0958
Carg03860	Melon (DHL92) v3.6.1	carmedB0960
Carg03860	Melon (DHL92) v3.6.1	carmedB0964
Carg03860	Watermelon (Charleston Gray)	carwcgB0959
Carg03860	Watermelon (97103) v1	carwmB0979
Carg03860	Watermelon (97103) v2	carwmbB1035
Carg03860	Wax gourd	carwgoB1144
Carg03860	Wax gourd	carwgoB1153