Bhi04G000897 (gene) Wax gourd

NameBhi04G000897
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCalcium-binding protein
Locationchr4 : 27428516 .. 27429399 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTCTTCTTCCTCGAAATTTAATTACAATTTTATATAATTAAATTTCAAATACAACGCATTCGATCCACAAAAAATCCCCCAATTTCTAAAACAATTATTTCTCTGCCAGCTATCAAATCTCCACCCTCACTCATAAATTCACCTCCCCCAATTCCCAAACACACATCACACACAACCCCTTCCAACCAATCTTGCCATTCCACTAAATGGGTATCTCCAGATTCATTGGGGACTTTCTACAAACATTTGGTGGGCGATCCCGATCAGCGGCGGAATCCACCATTGACGACGGCAAAACAAGGCCCCACAGAATGGTATACGACGACGTACTACTCAGGGCGTTGATTACGGTATTTGGGATGAGAGAAAACGGGAGGATCAAAAAGGAAAAGGCAAAAGGGGTGGTGGAGAAACTTGGGTTGATACAGAGTGAAGAAGAGAAGGCGAGGTTCGAGCTGGCGACGGCGGCGGAGGGCGGCGACGAGGTGGCGGTGGAGGAGGTGATCGGAGAGGAGGAAGGGAAGAGAAATGAATTGCTTTATGAAGCGTTTAAGATATTTGATGTAGATGGAGATGGGTTTATTGATACAGTGGAATTGAAAAGAGTGCTTGATTGTTTGGGGTTAGATAAAGGATGGGGAATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTTAATTTGGATGGTAAAGTTGATTTTAGTGAGTTTGAATTAATGATGGGTGTTAAATGCCTTTGATTTATTTTATGTTATTATCATTTTTTTCCCTTCTTTTTTTATTTATGTCAAAGTTCTACCAAAAAGTGGTAGTTTTGGCATAATCTTTGTCCAACTAAAGAAAATAAAATGAATAACATTCTTCCCTCAATTTCTACCA

mRNA sequence

CCTTCTTCTTCCTCGAAATTTAATTACAATTTTATATAATTAAATTTCAAATACAACGCATTCGATCCACAAAAAATCCCCCAATTTCTAAAACAATTATTTCTCTGCCAGCTATCAAATCTCCACCCTCACTCATAAATTCACCTCCCCCAATTCCCAAACACACATCACACACAACCCCTTCCAACCAATCTTGCCATTCCACTAAATGGGTATCTCCAGATTCATTGGGGACTTTCTACAAACATTTGGTGGGCGATCCCGATCAGCGGCGGAATCCACCATTGACGACGGCAAAACAAGGCCCCACAGAATGGTATACGACGACGTACTACTCAGGGCGTTGATTACGGTATTTGGGATGAGAGAAAACGGGAGGATCAAAAAGGAAAAGGCAAAAGGGGTGGTGGAGAAACTTGGGTTGATACAGAGTGAAGAAGAGAAGGCGAGGTTCGAGCTGGCGACGGCGGCGGAGGGCGGCGACGAGGTGGCGGTGGAGGAGGTGATCGGAGAGGAGGAAGGGAAGAGAAATGAATTGCTTTATGAAGCGTTTAAGATATTTGATGTAGATGGAGATGGGTTTATTGATACAGTGGAATTGAAAAGAGTGCTTGATTGTTTGGGGTTAGATAAAGGATGGGGAATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTTAATTTGGATGGTAAAGTTGATTTTAGTGAGTTTGAATTAATGATGGGTGTTAAATGCCTTTGATTTATTTTATGTTATTATCATTTTTTTCCCTTCTTTTTTTATTTATGTCAAAGTTCTACCAAAAAGTGGTAGTTTTGGCATAATCTTTGTCCAACTAAAGAAAATAAAATGAATAACATTCTTCCCTCAATTTCTACCA

Coding sequence (CDS)

ATGGGTATCTCCAGATTCATTGGGGACTTTCTACAAACATTTGGTGGGCGATCCCGATCAGCGGCGGAATCCACCATTGACGACGGCAAAACAAGGCCCCACAGAATGGTATACGACGACGTACTACTCAGGGCGTTGATTACGGTATTTGGGATGAGAGAAAACGGGAGGATCAAAAAGGAAAAGGCAAAAGGGGTGGTGGAGAAACTTGGGTTGATACAGAGTGAAGAAGAGAAGGCGAGGTTCGAGCTGGCGACGGCGGCGGAGGGCGGCGACGAGGTGGCGGTGGAGGAGGTGATCGGAGAGGAGGAAGGGAAGAGAAATGAATTGCTTTATGAAGCGTTTAAGATATTTGATGTAGATGGAGATGGGTTTATTGATACAGTGGAATTGAAAAGAGTGCTTGATTGTTTGGGGTTAGATAAAGGATGGGGAATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTTAATTTGGATGGTAAAGTTGATTTTAGTGAGTTTGAATTAATGATGGGTGTTAAATGCCTTTGA

Protein sequence

MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
BLAST of Bhi04G000897 vs. Swiss-Prot
Match: sp|Q9SU00|CML2_ARATH (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 9.7e-12
Identity = 45/136 (33.09%), Postives = 72/136 (52.94%), Query Frame = 0

Query: 43  LRALITVFGMRENGRIKKEKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIG- 102
           L  +  +F    +G+I K + K   + +G++  E E           G   + ++E    
Sbjct: 6   LSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSL 65

Query: 103 ----EEEGKRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVD 162
                EE +  E + EAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VD
Sbjct: 66  YQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 125

Query: 163 VNLDGKVDFSEFELMM 174
           V+ DG V+F EF+ MM
Sbjct: 126 VDGDGMVNFKEFKQMM 141

BLAST of Bhi04G000897 vs. Swiss-Prot
Match: sp|Q9FI19|CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-11
Identity = 50/146 (34.25%), Postives = 72/146 (49.32%), Query Frame = 0

Query: 43  LRALITVFGMRENGRIKKEKAKGVVEKLGL------IQSEEEKARFELATAAEGGDEVAV 102
           L  +  +F    +G I  E+    + +LGL      ++S  +       T     D  A+
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 103 EEVIGEE---------EGKRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIR 162
            + + E          +G     L EAF +FD DGDGFI  VEL++VL  LGL +   I 
Sbjct: 89  HKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIE 148

Query: 163 EIEKMVSVVDVNLDGKVDFSEFELMM 174
           ++EKM+  VD N DG+VDF EF+ MM
Sbjct: 149 QVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of Bhi04G000897 vs. Swiss-Prot
Match: sp|Q9SVG9|CML42_ARATH (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.3e-11
Identity = 35/63 (55.56%), Postives = 44/63 (69.84%), Query Frame = 0

Query: 111 LYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFE 170
           L EAFK+FD +GDGFI   EL+ VL  LGL +G  +  +EKM+  VD N DG+VDF EF+
Sbjct: 121 LAEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFK 180

Query: 171 LMM 174
            MM
Sbjct: 181 NMM 183

BLAST of Bhi04G000897 vs. Swiss-Prot
Match: sp|Q84UL5|CML32_ORYSJ (Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 GN=CML32 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-10
Identity = 49/156 (31.41%), Postives = 71/156 (45.51%), Query Frame = 0

Query: 41  VLLRALITVFGMRENGRIKKEKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEE-- 100
           V LR +  +F    +G I  ++    ++ LGL+   +  A    A   EG   +  E+  
Sbjct: 33  VRLRRVFDLFDRNGDGEITVDELAQALDALGLVADRDGLAATVSAYVPEGAAGLRFEDFD 92

Query: 101 ---------VIGEEEGKRNELLY------------EAFKIFDVDGDGFIDTVELKRVLDC 160
                    + G  +G                   EAFK+FDVDGDGFI   EL+ VL  
Sbjct: 93  ALHRALGDALFGSLDGXXXXXXXXXXXXXXXXXXREAFKVFDVDGDGFISASELQEVLKK 152

Query: 161 LGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMM 174
           LGL +   +  + +M+  VD N DG+VDF EF+ MM
Sbjct: 153 LGLPEAGSLATVREMICNVDRNSDGRVDFGEFKSMM 188

BLAST of Bhi04G000897 vs. Swiss-Prot
Match: sp|Q2QVI1|CML28_ORYSJ (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-10
Identity = 41/89 (46.07%), Postives = 55/89 (61.80%), Query Frame = 0

Query: 85  ATAAEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGW 144
           A AA GG+  A ++   E+EG R     EAF +FD +GDGFI   EL+ VL  LGL  G 
Sbjct: 82  AAAAIGGEGGAPDD---EDEGMR-----EAFNVFDQNGDGFITVDELRSVLSSLGLKHGR 141

Query: 145 GIREIEKMVSVVDVNLDGKVDFSEFELMM 174
              +  +M+S+VD + DG+VDF EF+ MM
Sbjct: 142 TADDCRRMISMVDADGDGRVDFKEFKQMM 162

BLAST of Bhi04G000897 vs. TAIR10
Match: AT4G12860.1 (EF hand calcium-binding protein family)

HSP 1 Score: 71.6 bits (174), Expect = 5.4e-13
Identity = 45/136 (33.09%), Postives = 72/136 (52.94%), Query Frame = 0

Query: 43  LRALITVFGMRENGRIKKEKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIG- 102
           L  +  +F    +G+I K + K   + +G++  E E           G   + ++E    
Sbjct: 6   LSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSL 65

Query: 103 ----EEEGKRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVD 162
                EE +  E + EAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VD
Sbjct: 66  YQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 125

Query: 163 VNLDGKVDFSEFELMM 174
           V+ DG V+F EF+ MM
Sbjct: 126 VDGDGMVNFKEFKQMM 141

BLAST of Bhi04G000897 vs. TAIR10
Match: AT5G44460.1 (calmodulin like 43)

HSP 1 Score: 70.5 bits (171), Expect = 1.2e-12
Identity = 50/146 (34.25%), Postives = 72/146 (49.32%), Query Frame = 0

Query: 43  LRALITVFGMRENGRIKKEKAKGVVEKLGL------IQSEEEKARFELATAAEGGDEVAV 102
           L  +  +F    +G I  E+    + +LGL      ++S  +       T     D  A+
Sbjct: 29  LHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFDDFAAL 88

Query: 103 EEVIGEE---------EGKRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIR 162
            + + E          +G     L EAF +FD DGDGFI  VEL++VL  LGL +   I 
Sbjct: 89  HKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIE 148

Query: 163 EIEKMVSVVDVNLDGKVDFSEFELMM 174
           ++EKM+  VD N DG+VDF EF+ MM
Sbjct: 149 QVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of Bhi04G000897 vs. TAIR10
Match: AT4G20780.1 (calmodulin like 42)

HSP 1 Score: 68.9 bits (167), Expect = 3.5e-12
Identity = 35/63 (55.56%), Postives = 44/63 (69.84%), Query Frame = 0

Query: 111 LYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFE 170
           L EAFK+FD +GDGFI   EL+ VL  LGL +G  +  +EKM+  VD N DG+VDF EF+
Sbjct: 121 LAEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFK 180

Query: 171 LMM 174
            MM
Sbjct: 181 NMM 183

BLAST of Bhi04G000897 vs. TAIR10
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 61.2 bits (147), Expect = 7.3e-10
Identity = 43/136 (31.62%), Postives = 68/136 (50.00%), Query Frame = 0

Query: 43  LRALITVFGMRENGRIKKEKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVI-- 102
           L A+   F +  +G+I  ++   ++  LG    EEE  +       +G   +  EE +  
Sbjct: 38  LEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVEL 97

Query: 103 ---GEEEGKRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVD 162
              G ++    E L +AF ++D+DG+G I   EL  VL  LG +    I E  KM+  VD
Sbjct: 98  NTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE--CSIAECRKMIGGVD 157

Query: 163 VNLDGKVDFSEFELMM 174
            + DG +DF EF++MM
Sbjct: 158 KDGDGTIDFEEFKIMM 171

BLAST of Bhi04G000897 vs. TAIR10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 61.2 bits (147), Expect = 7.3e-10
Identity = 42/116 (36.21%), Postives = 65/116 (56.03%), Query Frame = 0

Query: 58  IKKEKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGEEEGKRNELLYEAFKI 117
           I +E+A+ V+  LGL  ++++    E  +A E      V  +  E+E    E + +AF +
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQ--EQYSAKE------VSSLFEEKEASLEE-VKQAFDV 142

Query: 118 FDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMM 174
           FD + DGFID +EL+RVL  LG  +G  +     M+  +D N DGK+DF+EF   M
Sbjct: 143 FDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFM 189

BLAST of Bhi04G000897 vs. TrEMBL
Match: tr|A0A1S3BBS0|A0A1S3BBS0_CUCME (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103488209 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.3e-81
Identity = 161/178 (90.45%), Postives = 169/178 (94.94%), Query Frame = 0

Query: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GGRSR+AAESTID G+++P+RMVYDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV 120
           EKAKGVVEKLGLI   EEK +FELA A EGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELA-AGEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV 120

Query: 121 DGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 179
           DGDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 DGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

BLAST of Bhi04G000897 vs. TrEMBL
Match: tr|A0A0A0LP90|A0A0A0LP90_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G376820 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.1e-80
Identity = 159/178 (89.33%), Postives = 168/178 (94.38%), Query Frame = 0

Query: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GGRSR+A ESTID G+++P+RMVYDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAVESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV 120
           EKAKGVVEKLGLI   EEK +FELA A EGGDEVAVEE+IGEEEGKRNELLYEAFKIFDV
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELA-AGEGGDEVAVEEMIGEEEGKRNELLYEAFKIFDV 120

Query: 121 DGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 179
           DGDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 DGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

BLAST of Bhi04G000897 vs. TrEMBL
Match: tr|M5Y644|M5Y644_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G455200 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.4e-46
Identity = 113/181 (62.43%), Postives = 129/181 (71.27%), Query Frame = 0

Query: 4   SRFIGDFLQTFGGRSRSAAESTIDDG-KTRPHRMVYDDVLLRALITVFGMRENGRIKKEK 63
           SR +GD +Q FGG SR + E    +G          D+ + RALITVFGM  NGRIKKE 
Sbjct: 8   SRLLGDLVQAFGGVSRPSTECKPQNGXXXXXXXXXVDESMARALITVFGMETNGRIKKEN 67

Query: 64  AKGVVEKLGLIQSEEEKARFELATAAEGGD-EVAVEEVIG--EEE---GKRNELLYEAFK 123
           A+ VVE LGLI  EE+K+ F+L     G D EV VEEV+G  EEE    KRNELL EAFK
Sbjct: 68  ARRVVENLGLICHEEDKSSFDL----PGDDHEVPVEEVLGGLEEEDDGSKRNELLLEAFK 127

Query: 124 IFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVK 178
           IFD DGDGFI+ VELKRVL+CLGL  GW + +IEKMV VVDVNLDGKVDF+EFELMMGVK
Sbjct: 128 IFDEDGDGFIEAVELKRVLECLGLGSGWDMDQIEKMVRVVDVNLDGKVDFNEFELMMGVK 184

BLAST of Bhi04G000897 vs. TrEMBL
Match: tr|A0A2N9JA27|A0A2N9JA27_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS61285 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.5e-45
Identity = 111/179 (62.01%), Postives = 123/179 (68.72%), Query Frame = 0

Query: 4   SRFIGDFLQTF-GGRSRSAAESTIDDGKTRPHRMV-YDDVLLRALITVFGMRENGRIKKE 63
           SR IGD L  F   +  SAA    D    +PH     DD ++RALITVFGM  NGRIKKE
Sbjct: 8   SRLIGDLLHAFANSKPGSAAHLDKDQPTCKPHNACKVDDTMVRALITVFGMENNGRIKKE 67

Query: 64  KAKGVVEKLGLIQSEEE--KARFELATAAE---GGDEVAVEEVIG--EEEGKRNELLYEA 123
            A+ VVEKLGLI S EE  K  FEL        G DEV VEEV+G  E+  KRNELL EA
Sbjct: 68  NARRVVEKLGLIYSSEEDNKPSFELPNQGGLGLGDDEVPVEEVLGGLEDVSKRNELLQEA 127

Query: 124 FKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMM 174
           FKIFD DGDGFI+ VELKRVL+CLGLD GW + EI+KMV  VD+NLDGKVDF EFELMM
Sbjct: 128 FKIFDEDGDGFIEAVELKRVLECLGLDNGWDMGEIQKMVKAVDLNLDGKVDFGEFELMM 186

BLAST of Bhi04G000897 vs. TrEMBL
Match: tr|A0A2P4I020|A0A2P4I020_QUESU (Putative calcium-binding protein cml43 OS=Quercus suber OX=58331 GN=CFP56_79139 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.4e-44
Identity = 112/186 (60.22%), Postives = 129/186 (69.35%), Query Frame = 0

Query: 1   MGIS----RFIGDFLQTFGGRSRSAAESTIDDGKT-RPHRMV-YDDVLLRALITVFGMRE 60
           MGI+    R IGD L  FG    S+A    +D  T +P      DD ++RALITVFGM  
Sbjct: 1   MGITGVCIRLIGDMLHAFGNSKPSSAAHLDEDHSTCKPQNACKVDDTMVRALITVFGMEN 60

Query: 61  NGRIKKEKAKGVVEKLGL-IQSEEEKARFELATAAEG----GDEVAVEEVIG--EEEGKR 120
           NGRIKKE A+ VVEKLGL   SEE+K+ FEL    +G     DEV VEEV+G  E+  KR
Sbjct: 61  NGRIKKENARRVVEKLGLTYSSEEDKSSFELPGDQDGVGLEDDEVPVEEVLGGLEDMSKR 120

Query: 121 NELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFS 174
           NELL+EAFKIFD DG+GFI+ VELKRVLDCLGLD GW + EIEKMV  VD+N DGKVDF 
Sbjct: 121 NELLHEAFKIFDEDGNGFIEAVELKRVLDCLGLDCGWDMGEIEKMVKAVDLNFDGKVDFG 180

BLAST of Bhi04G000897 vs. NCBI nr
Match: XP_008445056.1 (PREDICTED: calmodulin-like protein 7 [Cucumis melo])

HSP 1 Score: 310.1 bits (793), Expect = 5.0e-81
Identity = 161/178 (90.45%), Postives = 169/178 (94.94%), Query Frame = 0

Query: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GGRSR+AAESTID G+++P+RMVYDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV 120
           EKAKGVVEKLGLI   EEK +FELA A EGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELA-AGEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV 120

Query: 121 DGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 179
           DGDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 DGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

BLAST of Bhi04G000897 vs. NCBI nr
Match: XP_004138884.1 (PREDICTED: calmodulin-like protein 2 [Cucumis sativus] >KGN62844.1 hypothetical protein Csa_2G376820 [Cucumis sativus])

HSP 1 Score: 307.4 bits (786), Expect = 3.2e-80
Identity = 159/178 (89.33%), Postives = 168/178 (94.38%), Query Frame = 0

Query: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GGRSR+A ESTID G+++P+RMVYDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAVESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV 120
           EKAKGVVEKLGLI   EEK +FELA A EGGDEVAVEE+IGEEEGKRNELLYEAFKIFDV
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELA-AGEGGDEVAVEEMIGEEEGKRNELLYEAFKIFDV 120

Query: 121 DGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 179
           DGDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 DGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

BLAST of Bhi04G000897 vs. NCBI nr
Match: XP_022951438.1 (calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.4 bits (747), Expect = 1.1e-75
Identity = 156/179 (87.15%), Postives = 163/179 (91.06%), Query Frame = 0

Query: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GGRSR AA+ST D G   PHR+++DDVLLRALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSR-AADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIG--EEEGKRNELLYEAFKIF 120
           EKAKGVVEKLGLI+SEEEK  FEL  A  GGDEVAVEEVIG  EEEGKRNELL+EAFKIF
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFEL-PADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIF 120

Query: 121 DVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 178
           DVDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 DVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 177

BLAST of Bhi04G000897 vs. NCBI nr
Match: XP_023002517.1 (calmodulin-like protein 2 [Cucurbita maxima])

HSP 1 Score: 286.2 bits (731), Expect = 7.7e-74
Identity = 154/179 (86.03%), Postives = 161/179 (89.94%), Query Frame = 0

Query: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GGRSR AA+S  D G   P R+++DDVLLRALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSR-AADSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIG--EEEGKRNELLYEAFKIF 120
           EKAKGVVEKLGLI+SEEEK  FEL  A  GGDEVAVEEVIG  EEEGKRNELL+EAFKIF
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFEL-PADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIF 120

Query: 121 DVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 178
           DVDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 DVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 177

BLAST of Bhi04G000897 vs. NCBI nr
Match: XP_022132261.1 (calmodulin-like protein 2 [Momordica charantia])

HSP 1 Score: 268.1 bits (684), Expect = 2.2e-68
Identity = 145/182 (79.67%), Postives = 153/182 (84.07%), Query Frame = 0

Query: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GG  R+AA+S      ++P    YDD LL ALITVFGMRENGRIKK
Sbjct: 72  MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 131

Query: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGE--EE---GKRNELLYEAF 120
           EKAKGVVEKLGLIQ EEEKARF+L    EGGDEVAVEEVIGE  EE   GK++ELL EAF
Sbjct: 132 EKAKGVVEKLGLIQGEEEKARFDL-PPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAF 191

Query: 121 KIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGV 178
           KIFDVDGDGFIDTVELKRVL CLGLDKGWGIREIEKMV VVDVNLDGKVDF EF+LMMGV
Sbjct: 192 KIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGV 251

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q9SU00|CML2_ARATH9.7e-1233.09Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1[more]
sp|Q9FI19|CML43_ARATH2.2e-1134.25Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ... [more]
sp|Q9SVG9|CML42_ARATH6.3e-1155.56Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
sp|Q84UL5|CML32_ORYSJ1.4e-1031.41Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
sp|Q2QVI1|CML28_ORYSJ1.8e-1046.07Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
AT4G12860.15.4e-1333.09EF hand calcium-binding protein family[more]
AT5G44460.11.2e-1234.25calmodulin like 43[more]
AT4G20780.13.5e-1255.56calmodulin like 42[more]
AT1G24620.17.3e-1031.62EF hand calcium-binding protein family[more]
AT3G29000.17.3e-1036.21Calcium-binding EF-hand family protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BBS0|A0A1S3BBS0_CUCME3.3e-8190.45calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103488209 PE=4 SV=1[more]
tr|A0A0A0LP90|A0A0A0LP90_CUCSA2.1e-8089.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G376820 PE=4 SV=1[more]
tr|M5Y644|M5Y644_PRUPE3.4e-4662.43Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G455200 PE=4 SV=1[more]
tr|A0A2N9JA27|A0A2N9JA27_FAGSY6.5e-4562.01Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS61285 PE=4 SV=1[more]
tr|A0A2P4I020|A0A2P4I020_QUESU1.4e-4460.22Putative calcium-binding protein cml43 OS=Quercus suber OX=58331 GN=CFP56_79139 ... [more]
Match NameE-valueIdentityDescription
XP_008445056.15.0e-8190.45PREDICTED: calmodulin-like protein 7 [Cucumis melo][more]
XP_004138884.13.2e-8089.33PREDICTED: calmodulin-like protein 2 [Cucumis sativus] >KGN62844.1 hypothetical ... [more]
XP_022951438.11.1e-7587.15calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like p... [more]
XP_023002517.17.7e-7486.03calmodulin-like protein 2 [Cucurbita maxima][more]
XP_022132261.12.2e-6879.67calmodulin-like protein 2 [Momordica charantia][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
IPR002048EF_hand_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M000897Bhi04M000897mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 148..176
e-value: 0.71
score: 18.9
coord: 110..138
e-value: 0.0018
score: 27.6
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 110..173
e-value: 9.8E-10
score: 38.7
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 144..178
score: 8.962
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 106..141
score: 14.151
IPR002048EF-hand domainCDDcd00051EFhcoord: 113..169
e-value: 3.07574E-13
score: 60.2541
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 9..176
e-value: 4.6E-21
score: 77.6
NoneNo IPR availablePANTHERPTHR45003FAMILY NOT NAMEDcoord: 95..173
NoneNo IPR availablePANTHERPTHR45003:SF2CALCIUM-BINDING PROTEIN CML42-RELATEDcoord: 95..173
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 119..131
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 50..173

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi04G000897Wax gourdwgowgoB090
Bhi04G000897Cucumber (Gy14) v1cgywgoB316
Bhi04G000897Cucumber (Gy14) v1cgywgoB628
Bhi04G000897Cucumber (Gy14) v1cgywgoB662
Bhi04G000897Cucumber (Gy14) v2cgybwgoB302
Bhi04G000897Cucurbita maxima (Rimu)cmawgoB0015
Bhi04G000897Cucurbita maxima (Rimu)cmawgoB0569
Bhi04G000897Cucurbita maxima (Rimu)cmawgoB0940
Bhi04G000897Cucurbita maxima (Rimu)cmawgoB0946
Bhi04G000897Cucurbita moschata (Rifu)cmowgoB0006
Bhi04G000897Cucurbita moschata (Rifu)cmowgoB0565
Bhi04G000897Cucurbita moschata (Rifu)cmowgoB0941
Bhi04G000897Cucurbita moschata (Rifu)cmowgoB0947
Bhi04G000897Cucurbita pepo (Zucchini)cpewgoB0138
Bhi04G000897Cucurbita pepo (Zucchini)cpewgoB0144
Bhi04G000897Cucurbita pepo (Zucchini)cpewgoB0688
Bhi04G000897Cucurbita pepo (Zucchini)cpewgoB0977
Bhi04G000897Wild cucumber (PI 183967)cpiwgoB314
Bhi04G000897Wild cucumber (PI 183967)cpiwgoB580
Bhi04G000897Cucumber (Chinese Long) v3cucwgoB319
Bhi04G000897Cucumber (Chinese Long) v3cucwgoB594
Bhi04G000897Cucumber (Chinese Long) v2cuwgoB312
Bhi04G000897Cucumber (Chinese Long) v2cuwgoB575
Bhi04G000897Bottle gourd (USVL1VR-Ls)lsiwgoB358
Bhi04G000897Bottle gourd (USVL1VR-Ls)lsiwgoB596
Bhi04G000897Melon (DHL92) v3.6.1medwgoB327
Bhi04G000897Melon (DHL92) v3.6.1medwgoB413
Bhi04G000897Melon (DHL92) v3.5.1mewgoB328
Bhi04G000897Melon (DHL92) v3.5.1mewgoB411
Bhi04G000897Watermelon (Charleston Gray)wcgwgoB615
Bhi04G000897Watermelon (97103) v2wgowmbB316
Bhi04G000897Watermelon (97103) v1wgowmB300
Bhi04G000897Silver-seed gourdcarwgoB0069
Bhi04G000897Silver-seed gourdcarwgoB0177
Bhi04G000897Silver-seed gourdcarwgoB0661
Bhi04G000897Silver-seed gourdcarwgoB0748