Carg02545 (gene) Silver-seed gourd

Overview
NameCarg02545
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionE3 ubiquitin-protein ligase RHA1B
LocationCucurbita_argyrosperma_scaffold_009 : 534934 .. 535470 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTTCCCTGCAAGCTACACTGAGCTGCTTCTCCCGAAGCTCTTCATCCATGTACTCTCCTTACTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTATTTTGCCTTCTAGGGCTCCAGGATTTTCTTGAACCGGACATTCCTTGGTCTAATCATTCTGATTCTTTTCCGACGGGACCATTGACGGACCTCGATCCTCTTTCCGCTGCTGTTCTCCGTGAGATTCTTCCTGTCGTCAGATTCTCTGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTTTGTCTCTACGAGTTCGAGACCGACGATGAGATCCGCCGCCTCGCGAACTGTCGCCACATCTTCCACCGCGGATGCCTCGATCGGTGGATCGGTTATGGTCAGAGGACCTGCCCGCTGTGCCGGACGGTGTTCATTCCGCCCGATTTGCGGACTGGTGGTTGTAATTTTGACGATAGGCCTTGGGAGGATTCGGAACTCTTCGAAGCACTCCACACTGATTCTTCTTCATCCTCGACGCTTCTCAGCGCTGGTTTGTAG

mRNA sequence

ATGGGCTTCCCTGCAAGCTACACTGAGCTGCTTCTCCCGAAGCTCTTCATCCATGTACTCTCCTTACTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTATTTTGCCTTCTAGGGCTCCAGGATTTTCTTGAACCGGACATTCCTTGGTCTAATCATTCTGATTCTTTTCCGACGGGACCATTGACGGACCTCGATCCTCTTTCCGCTGCTGTTCTCCGTGAGATTCTTCCTGTCGTCAGATTCTCTGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTTTGTCTCTACGAGTTCGAGACCGACGATGAGATCCGCCGCCTCGCGAACTGTCGCCACATCTTCCACCGCGGATGCCTCGATCGGTGGATCGGTTATGGTCAGAGGACCTGCCCGCTGTGCCGGACGGTGTTCATTCCGCCCGATTTGCGGACTGGTGGTTGTAATTTTGACGATAGGCCTTGGGAGGATTCGGAACTCTTCGAAGCACTCCACACTGATTCTTCTTCATCCTCGACGCTTCTCAGCGCTGGTTTGTAG

Coding sequence (CDS)

ATGGGCTTCCCTGCAAGCTACACTGAGCTGCTTCTCCCGAAGCTCTTCATCCATGTACTCTCCTTACTCGGCTTCCTCAGGAAGCTCATCTCCCTCTTATTTTGCCTTCTAGGGCTCCAGGATTTTCTTGAACCGGACATTCCTTGGTCTAATCATTCTGATTCTTTTCCGACGGGACCATTGACGGACCTCGATCCTCTTTCCGCTGCTGTTCTCCGTGAGATTCTTCCTGTCGTCAGATTCTCTGAACTTCTGGATCCGCCGGAGAGCTGCGCTGTTTGTCTCTACGAGTTCGAGACCGACGATGAGATCCGCCGCCTCGCGAACTGTCGCCACATCTTCCACCGCGGATGCCTCGATCGGTGGATCGGTTATGGTCAGAGGACCTGCCCGCTGTGCCGGACGGTGTTCATTCCGCCCGATTTGCGGACTGGTGGTTGTAATTTTGACGATAGGCCTTGGGAGGATTCGGAACTCTTCGAAGCACTCCACACTGATTCTTCTTCATCCTCGACGCTTCTCAGCGCTGGTTTGTAG

Protein sequence

MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGPLTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLDRWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL
Homology
[Close]
BLAST of Carg02545 vs. NCBI nr
Match: XP_022958594.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata])

HSP 1 Score: 370.2 bits (949), Expect = 4.0e-99
Identity = 177/178 (99.44%), Postives = 177/178 (99.44%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDR WEDSELFEALHTDSSSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRAWEDSELFEALHTDSSSSSTLLSAGL 178

[Close]
BLAST of Carg02545 vs. NCBI nr
Match: XP_022995549.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima] >XP_023533861.1 E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.6 bits (932), Expect = 3.8e-97
Identity = 177/180 (98.33%), Postives = 177/180 (98.33%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLF LLGLQDFLEPDIPWSNHSDSFPTGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFRLLGLQDFLEPDIPWSNHSDSFPTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSS--TLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSS  TLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTPTLLSAGL 180

[Close]
BLAST of Carg02545 vs. NCBI nr
Match: XP_023549736.1 (E3 ubiquitin-protein ligase RHA1B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 342.0 bits (876), Expect = 1.2e-90
Identity = 165/178 (92.70%), Postives = 171/178 (96.07%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS+LGFLRKLISLLF LLGLQDFLEPDIPW N SDSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FSELLDPPESCAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDR WEDSE+FEALH+DSSSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSDSSSSSTLLSAGL 178

[Close]
BLAST of Carg02545 vs. NCBI nr
Match: XP_022970110.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima])

HSP 1 Score: 334.7 bits (857), Expect = 1.9e-88
Identity = 163/178 (91.57%), Postives = 169/178 (94.94%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS+LGFLRKLISLLF LLGLQDFLEPDIPW N SDSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSILGFLRKLISLLFRLLGLQDFLEPDIPWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FSELLDPPESCAVCLYEFE+DDEIR LANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRHLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDR WEDSE+FEALH+D SSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSD-SSSSTLLSAGL 177

[Close]
BLAST of Carg02545 vs. NCBI nr
Match: XP_022928753.1 (E3 ubiquitin-protein ligase RHA1B [Cucurbita moschata])

HSP 1 Score: 332.4 bits (851), Expect = 9.4e-88
Identity = 163/178 (91.57%), Postives = 169/178 (94.94%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPASYTELLLPKLFIHVLS+LGFLRKLISLLF LLGLQDFLEPDI W N SDSF TGP
Sbjct: 1   MGFPASYTELLLPKLFIHVLSVLGFLRKLISLLFRLLGLQDFLEPDILWPNQSDSFTTGP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           LTDLDPLSAAVLREILPVV+FSELLDPPESCAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  LTDLDPLSAAVLREILPVVKFSELLDPPESCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIP DLR+G CNFDDR WEDSE+FEALH+D SSSSTLLSAGL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPGDLRSGVCNFDDRLWEDSEIFEALHSD-SSSSTLLSAGL 177

[Close]
BLAST of Carg02545 vs. TAIR10
Match: AT1G63840.1 (RING/U-box superfamily protein)

HSP 1 Score: 184.1 bits (466), Expect = 7.5e-47
Identity = 100/173 (57.80%), Postives = 119/173 (68.79%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFP  Y+ELLLPK+F ++LS LG +RKLIS +F ++GL DFLEP+      S S+P  P
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPE----PVSTSWPDPP 60

Query: 61  LTDLDPLSAAVLR-EILPVVRFSELLDP-PESCAVCLYEFETDDEIRRLANCRHIFHRGC 120
            T   P SAA+L  E+LPVVRFS++  P  E CAVCLY+FE DDEIRRL NCRHIFHRGC
Sbjct: 61  PTLTKPDSAAILAGEMLPVVRFSDINRPESECCAVCLYDFENDDEIRRLTNCRHIFHRGC 120

Query: 121 LDRW-IGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFEALHTDSSSS 171
           LDRW +GY Q TCPLCRT FIP  L+     F+ R W  S    +   D SSS
Sbjct: 121 LDRWMMGYNQMTCPLCRTQFIPDHLQ---LEFNQRLWSQSSAVSSQLLDESSS 166

[Close]
BLAST of Carg02545 vs. TAIR10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2)

HSP 1 Score: 177.2 bits (448), Expect = 9.1e-45
Identity = 86/161 (53.42%), Postives = 111/161 (68.94%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFP  YTE+ LPKLF+  LS+LGF+R ++  +F  LGL DFLE D  W +++ S+PT  
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYT-SYPTRI 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELL----DPPESCAVCLYEFETDDEIRRLANCRHIFHR 120
                P SA ++REILPV++F EL     D PE+CAVCLYEFE + EIR L NCRHIFHR
Sbjct: 61  PETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHR 120

Query: 121 GCLDRWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDS 158
            CLDRW+ + Q+TCPLCRT F+P +++     F+ R W  S
Sbjct: 121 SCLDRWMDHDQKTCPLCRTPFVPDEMQE---EFNQRLWAAS 157

[Close]
BLAST of Carg02545 vs. TAIR10
Match: AT5G41400.1 (RING/U-box superfamily protein)

HSP 1 Score: 160.6 bits (405), Expect = 8.8e-40
Identity = 87/174 (50.00%), Postives = 106/174 (60.92%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPD---------IPWSN 60
           MG+P  YTELLLP++F+H+LSLLG +R LI   F +LGL DFLE D              
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSXXXXXXXXXXX 60

Query: 61  HSDSFPTGPLTDLDPLSAAVLREILPVVRFSELLDP-----PESCAVCLYEFETDDEIRR 120
                          ++A +  EILPV+RFSEL  P      + CAVCL+EFE DDEIRR
Sbjct: 61  XXXXXXXXXXXXXXXVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LANCRHIFHRGCLDRW-IGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSEL 160
           L NC+HIFHR CLDRW +GY Q TCPLCRT FI  +L+     F+ R W +SEL
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVA---FNQRVWSESEL 171

[Close]
BLAST of Carg02545 vs. TAIR10
Match: AT4G11360.1 (RING-H2 finger A1B)

HSP 1 Score: 113.2 bits (282), Expect = 1.6e-25
Identity = 58/146 (39.73%), Postives = 85/146 (58.22%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MG P  + EL +P   +  L ++GF R ++  L   +GL  FL+       H+++  + P
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLD-------HNETSRSDP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELL-DPPESCAVCLYEFETDDEIRRLANCRHIFHRGCL 120
                  SA +  E++PVVRFS+LL DP + C VCL +F +DD+IR+L  C H+FH  CL
Sbjct: 61  TRLALSTSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWI-GYGQRTCPLCRTVFIPPDLRT 145
           DRWI    + TCP+CR  F+P +  T
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEEKST 139

[Close]
BLAST of Carg02545 vs. TAIR10
Match: AT4G11370.1 (RING-H2 finger A1A)

HSP 1 Score: 109.8 bits (273), Expect = 1.8e-24
Identity = 57/147 (38.78%), Postives = 86/147 (58.50%), Query Frame = 0 

Query: 1   MGFPASY-TELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTG 60
           MG P  + TEL +P   + +L ++GF R ++  L   +GL  FL+       H+++    
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLD-------HNETSAPD 60

Query: 61  PLTDLDPLSAAVLREILPVVRFSEL-LDPPESCAVCLYEFETDDEIRRLANCRHIFHRGC 120
                   SA++  E++PVVRFS+L  DP + C VCL +FE+DD++R+L  C H+FH  C
Sbjct: 61  LTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGQRTCPLCRTVFIPPDLRT 145
           LDRWI  Y +  CP+CR  F+P +  T
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKEKYT 140

[Close]
BLAST of Carg02545 vs. Swiss-Prot
Match: sp|Q9SUS5|RHA1B_ARATH (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.9e-24
Identity = 58/146 (39.73%), Postives = 85/146 (58.22%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MG P  + EL +P   +  L ++GF R ++  L   +GL  FL+       H+++  + P
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLD-------HNETSRSDP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELL-DPPESCAVCLYEFETDDEIRRLANCRHIFHRGCL 120
                  SA +  E++PVVRFS+LL DP + C VCL +F +DD+IR+L  C H+FH  CL
Sbjct: 61  TRLALSTSATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCL 120

Query: 121 DRWI-GYGQRTCPLCRTVFIPPDLRT 145
           DRWI    + TCP+CR  F+P +  T
Sbjct: 121 DRWIVDCNKITCPICRNRFLPEEKST 139

[Close]
BLAST of Carg02545 vs. Swiss-Prot
Match: sp|Q9SUS4|RHA1A_ARATH (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.2e-23
Identity = 57/147 (38.78%), Postives = 86/147 (58.50%), Query Frame = 0 

Query: 1   MGFPASY-TELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTG 60
           MG P  + TEL +P   + +L ++GF R ++  L   +GL  FL+       H+++    
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLD-------HNETSAPD 60

Query: 61  PLTDLDPLSAAVLREILPVVRFSEL-LDPPESCAVCLYEFETDDEIRRLANCRHIFHRGC 120
                   SA++  E++PVVRFS+L  DP + C VCL +FE+DD++R+L  C H+FH  C
Sbjct: 61  LTRHALSTSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYC 120

Query: 121 LDRWI-GYGQRTCPLCRTVFIPPDLRT 145
           LDRWI  Y +  CP+CR  F+P +  T
Sbjct: 121 LDRWIVDYNKMKCPVCRHRFLPKEKYT 140

[Close]
BLAST of Carg02545 vs. Swiss-Prot
Match: sp|Q9P7E1|YOF7_SCHPO (Uncharacterized RING finger protein P4H10.07 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBP4H10.07 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.7e-11
Identity = 27/47 (57.45%), Postives = 32/47 (68.09%), Query Frame = 0 

Query: 89  ESCAVCLYEFETDDEIRRLANCRHIFHRGCLDRWIGYGQRTCPLCRT 136
           E C VCL  FE +DE RRL  C H FHR C+D+W+   Q +CPLCRT
Sbjct: 523 ERCLVCLSNFELNDECRRLKQCNHFFHRECIDQWLTSSQNSCPLCRT 569

[Close]
BLAST of Carg02545 vs. Swiss-Prot
Match: sp|Q9LZJ6|ATL5_ARATH (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.7e-11
Identity = 37/85 (43.53%), Postives = 46/85 (54.12%), Query Frame = 0 

Query: 57  PTGPLTDLDPLSAAVLREILPVVRFSELL--DPPESCAVCLYEFETDDEIRRLANCRHIF 116
           PT   + L PL   VL +I P+  +S      P E C+VCL EFE DDE R L  C H+F
Sbjct: 78  PTQSSSSLSPLDPTVLEKI-PIFVYSVKTHESPLEECSVCLSEFEEDDEGRVLPKCGHVF 137

Query: 117 HRGCLDRWIGYGQRTCPLCRTVFIP 140
           H  C+D W    + +CPLCR    P
Sbjct: 138 HVDCIDTWF-RSRSSCPLCRAPVQP 160

[Close]
BLAST of Carg02545 vs. Swiss-Prot
Match: sp|Q570X5|ATL58_ARATH (RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.8e-11
Identity = 31/84 (36.90%), Postives = 51/84 (60.71%), Query Frame = 0 

Query: 57  PTGPLTDLDPLSAAVLREILPVVRFSELLDPPES-CAVCLYEFETDDEIRRLANCRHIFH 116
           P   L+ ++   +  LRE+LP+V F E     +S C+VCL +++ +D+++++  C+H FH
Sbjct: 65  PGNSLSTIELGLSKELREMLPIVVFKESFTVMDSQCSVCLGDYQPNDKLQQIPVCKHTFH 124

Query: 117 RGCLDRWIGYGQRTCPLCRTVFIP 140
             C+D W+     TCPLCR   IP
Sbjct: 125 MDCIDLWL-TSHTTCPLCRLALIP 147

[Close]
BLAST of Carg02545 vs. TrEMBL
Match: tr|A0A0A0KQV2|A0A0A0KQV2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G152910 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.0e-82
Identity = 149/179 (83.24%), Postives = 162/179 (90.50%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPA+YTELLLPKLFIH+LS LGFLRKLIS +F L GLQDFLEPDIPW N  DSFPT P
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFIFRLFGLQDFLEPDIPWPNPPDSFPTSP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVV+FS+LLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRT-GGCNFDDRPWEDSELFEALHTDSSSSSTLLSAGL 179
           RWIGYGQRTCPLCRTVFIPPDLR+ GGCNFDDR WEDSE+FE LH+DSSSS+ L + GL
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHSDSSSSNLLSTDGL 179

[Close]
BLAST of Carg02545 vs. TrEMBL
Match: tr|A0A1S3AU97|A0A1S3AU97_CUCME (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103482771 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.3e-81
Identity = 145/167 (86.83%), Postives = 154/167 (92.22%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFPA+YTELLLPKLFIH+LS LGFLRKLIS LF L GLQDFLEPDIPW N SDSFPT P
Sbjct: 1   MGFPATYTELLLPKLFIHLLSFLGFLRKLISFLFRLFGLQDFLEPDIPWPNPSDSFPTSP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
            T LDPLSAA+LREILPVV+FS+LLDPP+ CAVCLYEFE+DDEIRRLANCRHIFHRGCLD
Sbjct: 61  FTHLDPLSAALLREILPVVKFSDLLDPPDCCAVCLYEFESDDEIRRLANCRHIFHRGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRT-GGCNFDDRPWEDSELFEALHTD 167
           RWIGYGQRTCPLCRTVFIPPDLR+ GGCNFDDR WEDSE+FE LHTD
Sbjct: 121 RWIGYGQRTCPLCRTVFIPPDLRSGGGCNFDDRLWEDSEIFEPLHTD 167

[Close]
BLAST of Carg02545 vs. TrEMBL
Match: tr|M5WAZ7|M5WAZ7_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G104300 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.9e-52
Identity = 100/157 (63.69%), Postives = 122/157 (77.71%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFP  YTELLLPKLFIH LSLLG +RKLIS  F LLGLQDF++PD  W++     P  P
Sbjct: 1   MGFPVGYTELLLPKLFIHTLSLLGLIRKLISTFFSLLGLQDFIQPDNAWAD-----PPAP 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
             +    SA ++REILPVV+FS+L+DPPESCAVCLYEFE++DEIRRL NCRHIFH+GC+D
Sbjct: 61  FPEFHSASAVLIREILPVVKFSDLVDPPESCAVCLYEFESEDEIRRLTNCRHIFHKGCVD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDS 158
           RW+GY Q+TCPLCRT FI  D++    +F++R W  S
Sbjct: 121 RWVGYDQKTCPLCRTPFISDDMQG---DFNERLWAAS 149

[Close]
BLAST of Carg02545 vs. TrEMBL
Match: tr|A0A2I4H8Q8|A0A2I4H8Q8_9ROSI (E3 ubiquitin-protein ligase RHA1B-like OS=Juglans regia OX=51240 GN=LOC109014495 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.5e-52
Identity = 103/161 (63.98%), Postives = 122/161 (75.78%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFP  YTELLLPKLF+H LS+LGFLRK IS +F  LGL DFLEPDI W +  D  P   
Sbjct: 1   MGFPVGYTELLLPKLFLHTLSVLGFLRKFISTIFRYLGLGDFLEPDISWPDTPDRIP--- 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
             +   +SA ++REILPVVRFS+L+DPPESCAVCLYEFE+ DEIRRL NCRHIFH+GCLD
Sbjct: 61  --EFHSVSAVLIREILPVVRFSDLVDPPESCAVCLYEFESQDEIRRLTNCRHIFHKGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFE 162
           RW+GY Q+TCPLCRT FIP D++      ++R W  S + E
Sbjct: 121 RWMGYEQKTCPLCRTPFIPDDMQG---TINERLWAASGIPE 153

[Close]
BLAST of Carg02545 vs. TrEMBL
Match: tr|A0A2I4FCA2|A0A2I4FCA2_9ROSI (E3 ubiquitin-protein ligase RHA1B-like OS=Juglans regia OX=51240 GN=LOC108997441 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.5e-52
Identity = 104/161 (64.60%), Postives = 124/161 (77.02%), Query Frame = 0 

Query: 1   MGFPASYTELLLPKLFIHVLSLLGFLRKLISLLFCLLGLQDFLEPDIPWSNHSDSFPTGP 60
           MGFP  YT+LLLPKLFIH LS+LGFLRKLIS LF  LGL DFLEPDI W +         
Sbjct: 1   MGFPVGYTDLLLPKLFIHALSVLGFLRKLISTLFRHLGLADFLEPDIAWHD-----APAR 60

Query: 61  LTDLDPLSAAVLREILPVVRFSELLDPPESCAVCLYEFETDDEIRRLANCRHIFHRGCLD 120
           +++ + +SA ++REILPVVRFS+L+DPPESCAVCLYEFE  DEIRRL NCRHIFH+GCLD
Sbjct: 61  VSEFNSISAVLIREILPVVRFSDLVDPPESCAVCLYEFEPQDEIRRLTNCRHIFHKGCLD 120

Query: 121 RWIGYGQRTCPLCRTVFIPPDLRTGGCNFDDRPWEDSELFE 162
           RW+GY Q+TCPLCRT FIP D++     F++R W  S + E
Sbjct: 121 RWMGYEQKTCPLCRTPFIPDDMQGA---FNERLWAASGIPE 153

The following BLAST results are available for this feature:
BLAST of Carg02545 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022958594.14.0e-9999.44E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata][more]
XP_022995549.13.8e-9798.33E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima] >XP_023533861.1 E3 ubi... [more]
XP_023549736.11.2e-9092.70E3 ubiquitin-protein ligase RHA1B [Cucurbita pepo subsp. pepo][more]
XP_022970110.11.9e-8891.57E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima][more]
XP_022928753.19.4e-8891.57E3 ubiquitin-protein ligase RHA1B [Cucurbita moschata][more]
BLAST of Carg02545 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G63840.17.5e-4757.80RING/U-box superfamily protein[more]
AT3G61460.19.1e-4553.42brassinosteroid-responsive RING-H2[more]
AT5G41400.18.8e-4050.00RING/U-box superfamily protein[more]
AT4G11360.11.6e-2539.73RING-H2 finger A1B[more]
AT4G11370.11.8e-2438.78RING-H2 finger A1A[more]
BLAST of Carg02545 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9SUS5|RHA1B_ARATH2.9e-2439.73E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ... [more]
sp|Q9SUS4|RHA1A_ARATH3.2e-2338.78Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
sp|Q9P7E1|YOF7_SCHPO1.7e-1157.45Uncharacterized RING finger protein P4H10.07 OS=Schizosaccharomyces pombe (strai... [more]
sp|Q9LZJ6|ATL5_ARATH3.7e-1143.53RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1[more]
sp|Q570X5|ATL58_ARATH4.8e-1136.90RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1[more]
BLAST of Carg02545 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0KQV2|A0A0A0KQV2_CUCSA1.0e-8283.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G152910 PE=4 SV=1[more]
tr|A0A1S3AU97|A0A1S3AU97_CUCME3.3e-8186.83E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103482771 PE=4 S... [more]
tr|M5WAZ7|M5WAZ7_PRUPE1.9e-5263.69Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G104300 PE=4 SV=1[more]
tr|A0A2I4H8Q8|A0A2I4H8Q8_9ROSI2.5e-5263.98E3 ubiquitin-protein ligase RHA1B-like OS=Juglans regia OX=51240 GN=LOC109014495... [more]
tr|A0A2I4FCA2|A0A2I4FCA2_9ROSI2.5e-5264.60E3 ubiquitin-protein ligase RHA1B-like OS=Juglans regia OX=51240 GN=LOC108997441... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR001841Znf_RING
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02545-RACarg02545-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 91..133
e-value: 3.1E-6
score: 36.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 90..134
e-value: 1.1E-13
score: 51.2
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 91..134
score: 12.46
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 85..137
e-value: 1.3E-17
score: 65.1
NoneNo IPR availablePANTHERPTHR14155RING FINGER DOMAIN-CONTAININGcoord: 1..164
NoneNo IPR availablePANTHERPTHR14155:SF463SUBFAMILY NOT NAMEDcoord: 1..164
NoneNo IPR availableCDDcd16448RING-H2coord: 91..134
e-value: 3.02709E-13
score: 60.1583
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 72..140

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg02545Cla011427Watermelon (97103) v1carwmB0925
Carg02545Cla021223Watermelon (97103) v1carwmB0922
Carg02545Csa5G152910Cucumber (Chinese Long) v2carcuB0939
Carg02545MELO3C021190Melon (DHL92) v3.5.1carmeB0907
Carg02545MELO3C005696Melon (DHL92) v3.5.1carmeB0903
Carg02545ClCG06G003510Watermelon (Charleston Gray)carwcgB0904
Carg02545ClCG01G002500Watermelon (Charleston Gray)carwcgB0898
Carg02545CSPI05G05200Wild cucumber (PI 183967)carcpiB0961
Carg02545CSPI06G00710Wild cucumber (PI 183967)carcpiB0966
Carg02545Cucsa.135690Cucumber (Gy14) v1carcgyB1038
Carg02545Cucsa.303350Cucumber (Gy14) v1carcgyB1043
Carg02545CmaCh02G014340Cucurbita maxima (Rimu)carcmaB1268
Carg02545CmoCh02G014690Cucurbita moschata (Rifu)carcmoB1237
Carg02545CmoCh15G013080Cucurbita moschata (Rifu)carcmoB1230
Carg02545Lsi09G015870Bottle gourd (USVL1VR-Ls)carlsiB835
Carg02545Lsi09G002350Bottle gourd (USVL1VR-Ls)carlsiB836
Carg02545Cp4.1LG13g01360Cucurbita pepo (Zucchini)carcpeB1271
Carg02545CsGy5G002480Cucumber (Gy14) v2carcgybB0912
Carg02545CsGy6G000770Cucumber (Gy14) v2carcgybB0917
Carg02545MELO3C021190.2Melon (DHL92) v3.6.1carmedB0914
Carg02545MELO3C005696.2Melon (DHL92) v3.6.1carmedB0910
Carg02545CsaV3_5G002600Cucumber (Chinese Long) v3carcucB0963
Carg02545Cla97C01G002560Watermelon (97103) v2carwmbB0963
Carg02545Cla97C06G112880Watermelon (97103) v2carwmbB0970
Carg02545Bhi12G000272Wax gourdcarwgoB1059
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg02545Carg16622Silver-seed gourdcarcarB643
The following block(s) are covering this gene:
GeneOrganismBlock
Carg02545Cucurbita maxima (Rimu)carcmaB1261
Carg02545Cucurbita pepo (Zucchini)carcpeB1282