Carg01659 (gene) Silver-seed gourd

Overview
NameCarg01659
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionMitochondrial import inner membrane translocase subunit Tim21
LocationCucurbita_argyrosperma_scaffold_004 : 2232772 .. 2237505 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGACCGAAATGCCCCTCCGAATGAAGGGCTTCATCAGGGTTTAACACCTCTCCTAGGGTTTCTCTGTACAGATCAATTCTTCATCAGCTCTGAAAATCAATGGCGAACGGATTAATGAAGCTCACCGCGTTCGTCTCTCGGTCTTCTCTCGTCCACCGGCAATGGCACCAGTCCTCGCCATTTGCTCGTCTCGGTGTTCAGGAATATCTCCAGGTCACTTGCATTTCAATTTTGGTCTTTATTTTACTCTACTAGTTCTGTTTCTGACTGTCGAGTATTTGCTGGATCAGTGAGTACAATCGCTCTTGGTTCGGCCGCGACTTTAAGTTAGCTACGATGCACAAGATTACATTATGCTGATGTGATTTTTTCTGCGGTCTTTTAAGTATAAATTTGATTGTTCATAATGATAAGATGCCGAATTTGATGTCTCGAGTCGTTTCAATCATGTGGAAATCAGTGTAAAAGAAAATGCATAAGATATGGAGGTTACTTCTGCTGTGTTACAACGTACAGGGTTTTTTAATTTTTTTTTTCAAAGCAAAAAGAATCAAAGAACAATCTAATAACCAAAATGGACTCCCTTGAATGTTACAGTATATAATATTTAGTTCCTTGAAAGAAAAGTAGAAAAGAACTTGTCTGCAGATAATGGCATCGGATCATATGCAAATATATGCGTCATTTTTGGCCTCTTGAAGTGAAGTGATCAATATGCTTCTTCTTTCTCTCAATTTCAAGTTCAATATAGAAACCATTTGCCTTATCTGTATGTGCTAACTAGGGAGTTTTTTTTATTCTTTTCTTTCTTTTCCTTCTGTAGGACAGTGTTCTGCTCAACACAAGACTTTGGAATTTTAACTTTAGAATGAACTTAAGAGAGTGTGATATAGAGGGATTATAAATCCCGTTTTGGTCAGCTCACTCCATGTAACGGCCCAAGCCCACCGCTAGCAGATATTGTCCTCTTTGGGCTTTCCCTCAAGGCTTTAAAACGCGTATGCTAGGGGAATGTTTCCACACCCTTATAAATGGTGTTTTGTTCTCCTCTCCAACCAATGTGGGACATCACACTCCAGCTCTTGTTTCTTCCTAAGATGCTTGTCGAGGTTCTTTGGAAAGACAAATATCCTAATAACTAAAAAAATAAGGTAAGGTGTTCCTTCTCCTTCAAAAAGGAAAAATGTGCAGCCTTCTTTTCGGCAGTTTTTTTGTTTTTTTTAAGGTTTTGTGTACAATGAGATCATACAAACTAAAAAAACCTTCACGGGCTTTCAGTTCGACACCAATTATTTTTTGTTTATTATTATCTTTTCTTTTTTTTGGGTGGCGGGGGAGGGTAAAAATAGATGTTTAACCCTGTTAGATAGATGTAGGTATTGCTCAGGAATTTCTCTCATTCTAATTATGATCCTGCAATAGTTACCAAGATTCTTATCCAATGTAGAAGATTCTCTAATTTGGGATTTCTAACGACGATGTGTAATTGGTGCAGCTGCTTGGTGCACTGAAGCCTGGCATTGCCAAGCGATCTGTTGGGTACGTGTGTGATAGTCCTAAGGTGCAATAAGTCTTTCATGTTGGATGCTGATTTTTAGTTATTATTTCTTTTAGCATTCATTGAATCATTGGATGCCTGATTTTATTATTCATCAAATTTGCAGGCTGCACTTGGAGCTAATGGTTGGTTTTATTTACATTTATTTTTCTTGAACTTAGTTATTCTACCATGTTTCTTCCCCTGCAGGGGTGGGGGTGAGTTTGCAAAATGAGAAGTTTTTAAATTTGTGTGGATGATTGAATGATACTTGTGTAACTAGAGTTAATTAAAGCTGTATCCTTCCAAATTGCTTCTGGATCGTGGTTCATAACCCGCTCAAAGCTTCCCCAGAGAGATCTATTATTTTTTTAGTTTAAGGTTCACATATCTTATACGCGGGTTCGACACATTTGATCTAGTTCTTGTATATCTTATATGATGCTATTCTTCCCCATATATGACAATGACTTGAAGGAGTACAAAGGAATTTTCAAATTGTCTAATATTGTTATTGATTACCATGCGAAAAATGAGTTTCTAGGGGCTTGATGCTTTGATCAATTAGTCTAGTAACCCAATCGTTGGTCTCTCTCGCTTGGGACGGTGGCTGTTGGGCGTCCATCTCTTGCAGTTAACCTTTCCATATTATTATTTTTGTGCAGGTATGGCTGTTTTATCTAGACGGCAGGATGCACGGGGATCTGTTGTCAACTTTTCAACATTAGTTGTCACCAGTAGATGTGATACAACAGCTGGAGTGCCGTGCTTTGCTAGATTTATGTCATCAAAATCTTCAGAGCAACAGGGACAAAGCTCATCTCAGGTTTGTGTTCAAATTTGAGTTTGTTTACCATTGAAAGGGAGCTCTGGGTTTCTCTGGGTTCAATTTCTGAGGAAGGCTCTTTGACTGGGTCTAGATTCGGGTGTCTCAAATATATATATCGTCAAATGATCATGAAGATGACTGACTAGCCTTGTCATCTGAGAAAAAATGATCATCTCTTATAAATCTTAGATCTAATAAAGTAAGGTCATGGGGATAATTAGAATATCAATAATTTATGCAAGGTAAATCTAAGTTATGGTTAAACATTGTTGAGTGAATTTCTGTAATGTTTACTAATATATGAGTGAAAAGAAGTCGAGTAATTGTTTGGTTTCTTTTTTATCTGAATAATTGCATTGTACGTTACTAAAATTAACTTCAACTAGGCATGCGTCTCATTGGGATGTTGGTAAAAATTGAATCCGACTTAACTGAGCGTTGCCATACATACCTTTTATTAATTTCTTGCATGCATTCTAATTTATCGGTGTTCGCCTGGTGATGTCACGACTAGTTATTGATCACTGATGAGTTCATAAACCTGGATTTTCCCTTGATTTTGTTATCAGAGCAAAAAAGAAATATCAACAGTGGAGGATCCATTTGATGCTCCAACATATAATATACCAGAGAAGCCTGTTACATTTGCAGAGGGAGCTTCCTACAGTTTTATCATCCTTACAGGGCTTGGAGTTGCTGCAGCTGCCGGATATGCAGTTTTTAAGGAGCTTATATTTCAACCAAAAGAGTAACAATCAACTTACATTTAGATTAATGTTATAAACGTTTGCTTAGAATCATCCTTCAAATAGCTTGTGGTTTCCTCTACTTGGTGACTCTGATATGCAAATTTGTTTGCTTAGGTATAAGATCTTTGACAAGGCACTTAAAAGGATTCAAGATGACAGCCAGGTTAGTGAATTAACCTCGAACTTTTGTGTTTTGTATGTGTTCGTGTTAAAGAAACCAAATCCATTAAAGATAAACATGTCCATGAGAACAAAGATACCAAATGTAACATAAACGCAACCAAGGCACAATTACATCTGCAAAAAAGACCAAATGGCCGGAAAAGATAAACTAGGAACCTAGCAAGAAAACAAGAGGAACTAACCAAACTACAGCAGATCCTCAGGTAATCACCAACCAAGAGAACCACATAGTGGACCTCTTCCACTTTAATTCAAGGTACCCTCTAGATGGACCTATATTTTCTCCACCTACTATGAATTAGATATAGTTCATGTTTTTTCAGCACCATGTTCGGATTTTTCATTATGCAGCATCTTAAATATTTCTTGCACCTTAATTGAGTCAGAATAAGATGAATTTCACATCATGTCTTTTAGAGATGCAGGAACAGAATCTATCTATATATATATATATATATATATATAATGGTTCAGAAAGGTTGTTCTTGTTGTTAATTATGAAAAATAAGTTGGGTTCAAGGGGAAATTGATAGGCACAGATAGGGGGCAGTGGGTGGGCAATGAGATAGTTCTTATGCTCTTCTATTAAATTCAGACTATTGTGTAATTATTCCTTTTTTTCTGACTATAAGGATGTCTTATCCTTCTCTTCAGTTCTCTCTCATGATTTTCTTATTTAAAACAAATTCCCTTTTGTAATTAAGTTTCTGTTTCTTCTTTTAATAAAAATGTACCCTTTTGAGGTCTACTCAACAATTGGTATACTTATTTCAGTGTTCAAGTTATATCCTATGATCTTTTATGTTTTAATAGATTGATTTTGCTATTCTGTCAGGTTAAGGTAAGGATTGGTTCCCCTATAACTGGCTATGGCCAAGAAACTAGAAATCGGGCTGCTCGACAGCGTATCCCTAACAGGGTATGGACCGATGAAGATGGCGTCGAGAGAGTAGAGGTAGGTAATTCATGACTTAAATTGTGATAAGGTTTTCCTGTTGTGGTAAAAACAATTTCCCCTTCCCATTAGAAATTAATTAACAAATAAATGTTGTACAGGTGAATTTTTATATCCGTGGGCCCCATGGAGGTGGGAAAGTTTATACCGAGATGTTCAAGGATCAAGTGGAGAAGCAGTGGAAGTTCACATATTTGATTGTGGAGGTGAAATCTTCTCCTCCAGTTCAATTGATCCTAGAATCTTATATGCCTGCCTGACTGACTTTGGGCAACCAATTCATTCGCTCAAGATTTTGTATCAAATTCCACATAGTTTTAGAATTATCATGTTTATAATATTTCTCCTTAGCTCTTACCACATTTTAAACAGTCAATTCGGTTTGGCCAACTTTTTCGAGTTCATTATTTATGATTTCCTCCTATGGATGTGTTCCATTCCAATAAATAAATACCGATTCAACTTACTTCCACTTGGT

mRNA sequence

TTTTGACCGAAATGCCCCTCCGAATGAAGGGCTTCATCAGGGTTTAACACCTCTCCTAGGGTTTCTCTGTACAGATCAATTCTTCATCAGCTCTGAAAATCAATGGCGAACGGATTAATGAAGCTCACCGCGTTCGTCTCTCGGTCTTCTCTCGTCCACCGGCAATGGCACCAGTCCTCGCCATTTGCTCGTCTCGGTGTTCAGGAATATCTCCAGCTGCTTGGTGCACTGAAGCCTGGCATTGCCAAGCGATCTGTTGGGTACGTGTGTGATAGTCCTAAGGCTGCACTTGGAGCTAATGGTATGGCTGTTTTATCTAGACGGCAGGATGCACGGGGATCTGTTGTCAACTTTTCAACATTAGTTGTCACCAGTAGATGTGATACAACAGCTGGAGTGCCGTGCTTTGCTAGATTTATGTCATCAAAATCTTCAGAGCAACAGGGACAAAGCTCATCTCAGAGCAAAAAAGAAATATCAACAGTGGAGGATCCATTTGATGCTCCAACATATAATATACCAGAGAAGCCTGTTACATTTGCAGAGGGAGCTTCCTACAGTTTTATCATCCTTACAGGGCTTGGAGTTGCTGCAGCTGCCGGATATGCAAATCATCCTTCAAATAGCTTGTGGTTTCCTCTACTTGGTGACTCTGATATGCAAATTTGTTTGCTTAGGTATAAGATCTTTGACAAGGCACTTAAAAGGATTCAAGATGACAGCCAGGTTAAGGTAAGGATTGGTTCCCCTATAACTGGCTATGGCCAAGAAACTAGAAATCGGGCTGCTCGACAGCGTATCCCTAACAGGGTATGGACCGATGAAGATGGCGTCGAGAGAGTAGAGGTGAATTTTTATATCCGTGGGCCCCATGGAGGTGGGAAAGTTTATACCGAGATGTTCAAGGATCAAGTGGAGAAGCAGTGGAAGTTCACATATTTGATTGTGGAGGTGAAATCTTCTCCTCCAGTTCAATTGATCCTAGAATCTTATATGCCTGCCTGACTGACTTTGGGCAACCAATTCATTCGCTCAAGATTTTGTATCAAATTCCACATAGTTTTAGAATTATCATGTTTATAATATTTCTCCTTAGCTCTTACCACATTTTAAACAGTCAATTCGGTTTGGCCAACTTTTTCGAGTTCATTATTTATGATTTCCTCCTATGGATGTGTTCCATTCCAATAAATAAATACCGATTCAACTTACTTCCACTTGGT

Coding sequence (CDS)

ATGGCGAACGGATTAATGAAGCTCACCGCGTTCGTCTCTCGGTCTTCTCTCGTCCACCGGCAATGGCACCAGTCCTCGCCATTTGCTCGTCTCGGTGTTCAGGAATATCTCCAGCTGCTTGGTGCACTGAAGCCTGGCATTGCCAAGCGATCTGTTGGGTACGTGTGTGATAGTCCTAAGGCTGCACTTGGAGCTAATGGTATGGCTGTTTTATCTAGACGGCAGGATGCACGGGGATCTGTTGTCAACTTTTCAACATTAGTTGTCACCAGTAGATGTGATACAACAGCTGGAGTGCCGTGCTTTGCTAGATTTATGTCATCAAAATCTTCAGAGCAACAGGGACAAAGCTCATCTCAGAGCAAAAAAGAAATATCAACAGTGGAGGATCCATTTGATGCTCCAACATATAATATACCAGAGAAGCCTGTTACATTTGCAGAGGGAGCTTCCTACAGTTTTATCATCCTTACAGGGCTTGGAGTTGCTGCAGCTGCCGGATATGCAAATCATCCTTCAAATAGCTTGTGGTTTCCTCTACTTGGTGACTCTGATATGCAAATTTGTTTGCTTAGGTATAAGATCTTTGACAAGGCACTTAAAAGGATTCAAGATGACAGCCAGGTTAAGGTAAGGATTGGTTCCCCTATAACTGGCTATGGCCAAGAAACTAGAAATCGGGCTGCTCGACAGCGTATCCCTAACAGGGTATGGACCGATGAAGATGGCGTCGAGAGAGTAGAGGTGAATTTTTATATCCGTGGGCCCCATGGAGGTGGGAAAGTTTATACCGAGATGTTCAAGGATCAAGTGGAGAAGCAGTGGAAGTTCACATATTTGATTGTGGAGGTGAAATCTTCTCCTCCAGTTCAATTGATCCTAGAATCTTATATGCCTGCCTGA

Protein sequence

MANGLMKLTAFVSRSSLVHRQWHQSSPFARLGVQEYLQLLGALKPGIAKRSVGYVCDSPKAALGANGMAVLSRRQDARGSVVNFSTLVVTSRCDTTAGVPCFARFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILTGLGVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKRIQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVEVKSSPPVQLILESYMPA
Homology
[Close]
BLAST of Carg01659 vs. NCBI nr
Match: XP_022939739.1 (probable mitochondrial import inner membrane translocase subunit TIM21 isoform X1 [Cucurbita moschata])

HSP 1 Score: 515.4 bits (1326), Expect = 1.3e-142
Identity = 268/300 (89.33%), Postives = 274/300 (91.33%), Query Frame = 0 

Query: 1   MANGLMKLTAFVSRSSLVHRQWHQSSPFARLGVQEYLQLLGALKPGIAKRSVGYVCDSPK 60
           MANGLMKLTAFVSRS LVHR+WHQSSPF+RLGVQEYLQLLGALKPGIA+ SVGYVCDSPK
Sbjct: 1   MANGLMKLTAFVSRSPLVHRKWHQSSPFSRLGVQEYLQLLGALKPGIAEPSVGYVCDSPK 60

Query: 61  AALGANGMAVLSRRQDARGSVVNFSTLVVTSRCDTTAGVPCFARFMSSKSSEQQGQSSSQ 120
           AALGANGMAVLSRRQDARGSVVNFSTL VTSRCDTTAGVPCFARFMSSKSSEQ+GQSSSQ
Sbjct: 61  AALGANGMAVLSRRQDARGSVVNFSTLDVTSRCDTTAGVPCFARFMSSKSSEQRGQSSSQ 120

Query: 121 SKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILTGLGVAAAAGYANHPSNSLWFPL 180
           SKKEISTVEDPFDAPTYNIPEKPVTF EGASYSFIIL GLGVAAAAGYA           
Sbjct: 121 SKKEISTVEDPFDAPTYNIPEKPVTFTEGASYSFIILAGLGVAAAAGYAVF--------- 180

Query: 181 LGDSDMQICLLRYKIFDKALKRIQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTD 240
               ++      YKIFDKALKRIQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTD
Sbjct: 181 ---KELIFQPKEYKIFDKALKRIQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTD 240

Query: 241 EDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVEVKSSPPVQLILESYMPA 300
           EDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVEVKSSPPVQLILESYMPA
Sbjct: 241 EDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVEVKSSPPVQLILESYMPA 288

[Close]
BLAST of Carg01659 vs. NCBI nr
Match: XP_023550442.1 (probable mitochondrial import inner membrane translocase subunit TIM21 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 510.4 bits (1313), Expect = 4.2e-141
Identity = 266/300 (88.67%), Postives = 272/300 (90.67%), Query Frame = 0 

Query: 1   MANGLMKLTAFVSRSSLVHRQWHQSSPFARLGVQEYLQLLGALKPGIAKRSVGYVCDSPK 60
           MA GLMKLT FV RSSLVHRQWHQSS F+RLGVQEYLQLLG LKPGIA++SVGYVCDSPK
Sbjct: 1   MATGLMKLTTFVFRSSLVHRQWHQSSSFSRLGVQEYLQLLGELKPGIAEQSVGYVCDSPK 60

Query: 61  AALGANGMAVLSRRQDARGSVVNFSTLVVTSRCDTTAGVPCFARFMSSKSSEQQGQSSSQ 120
           AALGANGMAVLSRRQDARGS VNFSTLVVTSRCDTTAGVPCFARFMSSKSSEQ+GQSSSQ
Sbjct: 61  AALGANGMAVLSRRQDARGSGVNFSTLVVTSRCDTTAGVPCFARFMSSKSSEQRGQSSSQ 120

Query: 121 SKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILTGLGVAAAAGYANHPSNSLWFPL 180
           SKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIIL GLGVAAAAGYA           
Sbjct: 121 SKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILAGLGVAAAAGYAVF--------- 180

Query: 181 LGDSDMQICLLRYKIFDKALKRIQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTD 240
               ++      YKIFDKALKRIQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTD
Sbjct: 181 ---KELIFQPKEYKIFDKALKRIQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTD 240

Query: 241 EDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVEVKSSPPVQLILESYMPA 300
           EDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVEVKSSPPVQLILESYMPA
Sbjct: 241 EDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVEVKSSPPVQLILESYMPA 288

[Close]
BLAST of Carg01659 vs. NCBI nr
Match: XP_022992662.1 (probable mitochondrial import inner membrane translocase subunit TIM21 isoform X2 [Cucurbita maxima])

HSP 1 Score: 444.1 bits (1141), Expect = 3.7e-121
Identity = 248/328 (75.61%), Postives = 257/328 (78.35%), Query Frame = 0 

Query: 1   MANGLMKLTAFVSRSSLVHRQWHQSSPFARLGVQEYLQL--------------------- 60
           MANGLMKL AFV RSSLVHRQWHQSS F+RLGVQEYLQ+                     
Sbjct: 1   MANGLMKLAAFVPRSSLVHRQWHQSSSFSRLGVQEYLQVLLRNFSHSNYDPALVTKILIQ 60

Query: 61  ------LGALKPGIAKRSVGYVCDS-PKAALGANGMAVLSRRQDARGSVVNFSTLVVTSR 120
                 LG LK      +  + C +   AALGANGMAV SR Q ARGSVVNFSTLVVT+R
Sbjct: 61  CRRFSNLGFLKTMCNWCTEAWHCQAICWAALGANGMAVSSRLQAARGSVVNFSTLVVTNR 120

Query: 121 CDTTAGVPCFARFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASY 180
           CDTTAGVPCFARFMSSKSSEQ+GQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASY
Sbjct: 121 CDTTAGVPCFARFMSSKSSEQRGQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASY 180

Query: 181 SFIILTGLGVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKRIQDDSQVKVR 240
           SFIIL GLGVAAAAGYA               ++      YKIFDKALKRIQDDSQVKVR
Sbjct: 181 SFIILAGLGVAAAAGYAVF------------KELIFQPKEYKIFDKALKRIQDDSQVKVR 240

Query: 241 IGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKVYTEMFKDQVE 300
           IGSPITGYGQETRNRAARQ IPNRVWTDEDGVERVEVNFYIRGPHGGGKV TEMFKDQVE
Sbjct: 241 IGSPITGYGQETRNRAARQCIPNRVWTDEDGVERVEVNFYIRGPHGGGKVSTEMFKDQVE 300

[Close]
BLAST of Carg01659 vs. NCBI nr
Match: XP_022992663.1 (probable mitochondrial import inner membrane translocase subunit TIM21 isoform X3 [Cucurbita maxima])

HSP 1 Score: 437.2 bits (1123), Expect = 4.5e-119
Identity = 231/262 (88.17%), Postives = 235/262 (89.69%), Query Frame = 0 

Query: 39  LLGALKPGIAKRSVGYVCDSPKAALGANGMAVLSRRQDARGSVVNFSTLVVTSRCDTTAG 98
           LLGALKPGIAKRSVGYVCDSPKAALGANGMAV SR Q ARGSVVNFSTLVVT+RCDTTAG
Sbjct: 20  LLGALKPGIAKRSVGYVCDSPKAALGANGMAVSSRLQAARGSVVNFSTLVVTNRCDTTAG 79

Query: 99  VPCFARFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILT 158
           VPCFARFMSSKSSEQ+GQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIIL 
Sbjct: 80  VPCFARFMSSKSSEQRGQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILA 139

Query: 159 GLGVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKRIQDDSQVKVRIGSPIT 218
           GLGVAAAAGYA               ++      YKIFDKALKRIQDDSQVKVRIGSPIT
Sbjct: 140 GLGVAAAAGYAVF------------KELIFQPKEYKIFDKALKRIQDDSQVKVRIGSPIT 199

Query: 219 GYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFT 278
           GYGQETRNRAARQ IPNRVWTDEDGVERVEVNFYIRGPHGGGKV TEMFKDQVEKQWKFT
Sbjct: 200 GYGQETRNRAARQCIPNRVWTDEDGVERVEVNFYIRGPHGGGKVSTEMFKDQVEKQWKFT 259

Query: 279 YLIVEVKSSPPVQLILESYMPA 301
           YLIVEVKSSPPVQLILESYMPA
Sbjct: 260 YLIVEVKSSPPVQLILESYMPA 269

[Close]
BLAST of Carg01659 vs. NCBI nr
Match: XP_022990405.1 (probable mitochondrial import inner membrane translocase subunit TIM21 [Cucurbita maxima] >XP_022990406.1 probable mitochondrial import inner membrane translocase subunit TIM21 [Cucurbita maxima])

HSP 1 Score: 436.0 bits (1120), Expect = 1.0e-118
Identity = 237/337 (70.33%), Postives = 250/337 (74.18%), Query Frame = 0 

Query: 1   MANGLMKLTAFVSRSSLVHRQWHQSSPFARLGVQEYL----------------------- 60
           MANGLMKLT FVSRS +V RQWHQSS F+RL   EYL                       
Sbjct: 1   MANGLMKLTTFVSRSFIVPRQWHQSSSFSRLVAHEYLKAAEVISRSGMNISKGVAVPTID 60

Query: 61  --------------QLLGALKPGIAKRSVGYVCDSPKAALGANGMAVLSRRQDARGSVVN 120
                         +LLG LK G+AKRS GYVC+ PK  LGA GMAVLSRRQ ARGS+VN
Sbjct: 61  RAKRALPDTYVTVRELLGPLKSGVAKRSAGYVCNIPKDTLGATGMAVLSRRQGARGSIVN 120

Query: 121 FSTLVVTSRCDTTAGVPCFARFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEKP 180
            S L VTSRCDT AGV CFARFMSSKSSEQ+GQ+ S+ KKEISTVEDPFDAPTYNIPEKP
Sbjct: 121 LSPLAVTSRCDTNAGVACFARFMSSKSSEQRGQTPSERKKEISTVEDPFDAPTYNIPEKP 180

Query: 181 VTFAEGASYSFIILTGLGVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKRI 240
           VTFAEGASYSFIIL GLGVAAAAGYA               ++      YKIF KALKRI
Sbjct: 181 VTFAEGASYSFIILAGLGVAAAAGYAVF------------KELIFQPKEYKIFGKALKRI 240

Query: 241 QDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKVY 300
           QDDSQV+VRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHG GKVY
Sbjct: 241 QDDSQVRVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGAGKVY 300

[Close]
BLAST of Carg01659 vs. TAIR10
Match: AT4G00026.1 (FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 261.2 bits (666), Expect = 8.1e-70
Identity = 143/257 (55.64%), Postives = 173/257 (67.32%), Query Frame = 0 

Query: 46  GIAKRSVGYVCDS--PKAALGANGMAVLSRRQDARGSVVNFSTLVVTSRCDTTAGVPCFA 105
           GI K S     +S   K + GANG+    R   A          + T           F 
Sbjct: 34  GIPKSSKRVFSNSFLSKDSTGANGLLFRFRNPQAS---------ICTEARPKNINSSYFT 93

Query: 106 RFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILTGLGVA 165
           R  +S++S++ G   +++KKE++TVEDPFD+PTY+IPEKPVTF EGASYS +IL GLGVA
Sbjct: 94  RSFASRTSKEPGNQQNKAKKEVTTVEDPFDSPTYHIPEKPVTFTEGASYSLVILAGLGVA 153

Query: 166 AAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKRIQDDSQVKVRIGSPITGYGQE 225
            AAGY                ++      YK+FDKALKRIQDD QV+VRIGSPI GYGQE
Sbjct: 154 GAAGYGVF------------KELIFQPKEYKVFDKALKRIQDDGQVRVRIGSPIKGYGQE 213

Query: 226 TRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVE 285
           TRNRAARQRIPNRV+TDEDGVE VEVNFYIRGP G GKVYTEMFKD+ EK+WK+TYLIVE
Sbjct: 214 TRNRAARQRIPNRVFTDEDGVEHVEVNFYIRGPQGAGKVYTEMFKDKAEKEWKYTYLIVE 269

Query: 286 VKSSPPVQLILESYMPA 301
           + +  P +L+LESY+PA
Sbjct: 274 ILTPSPAKLMLESYLPA 269

[Close]
BLAST of Carg01659 vs. Swiss-Prot
Match: sp|Q1G3L1|TIM21_ARATH (Probable mitochondrial import inner membrane translocase subunit TIM21 OS=Arabidopsis thaliana OX=3702 GN=TIM21 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.5e-68
Identity = 143/257 (55.64%), Postives = 173/257 (67.32%), Query Frame = 0 

Query: 46  GIAKRSVGYVCDS--PKAALGANGMAVLSRRQDARGSVVNFSTLVVTSRCDTTAGVPCFA 105
           GI K S     +S   K + GANG+    R   A          + T           F 
Sbjct: 34  GIPKSSKRVFSNSFLSKDSTGANGLLFRFRNPQAS---------ICTEARPKNINSSYFT 93

Query: 106 RFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILTGLGVA 165
           R  +S++S++ G   +++KKE++TVEDPFD+PTY+IPEKPVTF EGASYS +IL GLGVA
Sbjct: 94  RSFASRTSKEPGNQQNKAKKEVTTVEDPFDSPTYHIPEKPVTFTEGASYSLVILAGLGVA 153

Query: 166 AAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKRIQDDSQVKVRIGSPITGYGQE 225
            AAGY                ++      YK+FDKALKRIQDD QV+VRIGSPI GYGQE
Sbjct: 154 GAAGYGVF------------KELIFQPKEYKVFDKALKRIQDDGQVRVRIGSPIKGYGQE 213

Query: 226 TRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYLIVE 285
           TRNRAARQRIPNRV+TDEDGVE VEVNFYIRGP G GKVYTEMFKD+ EK+WK+TYLIVE
Sbjct: 214 TRNRAARQRIPNRVFTDEDGVEHVEVNFYIRGPQGAGKVYTEMFKDKAEKEWKYTYLIVE 269

Query: 286 VKSSPPVQLILESYMPA 301
           + +  P +L+LESY+PA
Sbjct: 274 ILTPSPAKLMLESYLPA 269

[Close]
BLAST of Carg01659 vs. TrEMBL
Match: tr|A0A1S3C3T0|A0A1S3C3T0_CUCME (probable mitochondrial import inner membrane translocase subunit TIM21 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496532 PE=4 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 3.1e-116
Identity = 233/338 (68.93%), Postives = 252/338 (74.56%), Query Frame = 0 

Query: 1   MANGLMKLTAFVSRSSLVHRQWHQSSPFARLGVQEYLQ---------------------- 60
           MANGL+K T F+S SSL+ RQWH SS F+RLG  E+LQ                      
Sbjct: 1   MANGLLKFTHFISPSSLLPRQWHHSSTFSRLGSHEFLQTAEVMRSSGTNASKRVAVNIID 60

Query: 61  ----------------LLGALKPGIAKRSVGYVCDSPKAALGANGMAVLSRRQDARGSVV 120
                           LLG LK GI+K+S  YVCD PKAA+G NG+ VLSR Q+ R SVV
Sbjct: 61  EAKRGLPDTYATSVRELLGPLKFGISKQSAVYVCDIPKAAIGGNGIPVLSRWQNVRASVV 120

Query: 121 NFSTLVVTSRCDTTAGVPCFARFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEK 180
           NFSTL VTSRCDT+A  PCFARFMSSKSSE++GQ+ S+SKKEISTVEDPFDAPTYNIPEK
Sbjct: 121 NFSTLGVTSRCDTSARGPCFARFMSSKSSEKRGQTESESKKEISTVEDPFDAPTYNIPEK 180

Query: 181 PVTFAEGASYSFIILTGLGVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKR 240
           PVTFAEGASYSFIIL GLGVAAAAGYA               ++      YKIFDKALKR
Sbjct: 181 PVTFAEGASYSFIILAGLGVAAAAGYAVF------------KELIFQPKEYKIFDKALKR 240

Query: 241 IQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKV 300
           IQDDSQV+VRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHG GKV
Sbjct: 241 IQDDSQVRVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGAGKV 300

[Close]
BLAST of Carg01659 vs. TrEMBL
Match: tr|A0A0A0K9E8|A0A0A0K9E8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G046410 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 4.5e-115
Identity = 237/338 (70.12%), Postives = 252/338 (74.56%), Query Frame = 0 

Query: 1   MANGLMKLTAFVSRSSLVHRQWHQSSPFARLGVQEYLQ---------------------- 60
           MANGLMKLT F+S SSL+ RQWH SS F+RLG  E+LQ                      
Sbjct: 1   MANGLMKLTRFISPSSLLPRQWHHSS-FSRLGSHEFLQTAEVMRSSGMNASKRVTVNIID 60

Query: 61  ----------------LLGALKPGIAKRSVGYVCDSPKAALGANGMAVLSRRQDARGSVV 120
                           LL  LK GI+K+S  YVCD PKAALG  GM VLSR QDAR SVV
Sbjct: 61  EAKSGLPDTYATSVRELLAPLKSGISKQSAVYVCDIPKAALG--GMPVLSRWQDARASVV 120

Query: 121 NFSTLVVTSRCDTTAGVPCFARFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEK 180
           NFSTL VTSRCDT+A  PCFARFMSSKSSE++GQ+ S+SKKEISTVEDPFDAPTYNIPEK
Sbjct: 121 NFSTLGVTSRCDTSARGPCFARFMSSKSSEKRGQTESESKKEISTVEDPFDAPTYNIPEK 180

Query: 181 PVTFAEGASYSFIILTGLGVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKR 240
           PVTFAEGASYSFIIL GLGVAAAAGYA               ++      YKIFDKALKR
Sbjct: 181 PVTFAEGASYSFIILAGLGVAAAAGYAVF------------KELIFQPKEYKIFDKALKR 240

Query: 241 IQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKV 300
           IQDDSQV+VRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHG GKV
Sbjct: 241 IQDDSQVRVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGAGKV 300

[Close]
BLAST of Carg01659 vs. TrEMBL
Match: tr|A0A1S3C3P7|A0A1S3C3P7_CUCME (probable mitochondrial import inner membrane translocase subunit TIM21 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496532 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 5.0e-114
Identity = 232/338 (68.64%), Postives = 251/338 (74.26%), Query Frame = 0 

Query: 1   MANGLMKLTAFVSRSSLVHRQWHQSSPFARLGVQEYLQ---------------------- 60
           MANGL+K T F+S SSL+ RQWH SS F+RLG  E+LQ                      
Sbjct: 1   MANGLLKFTHFISPSSLLPRQWHHSSTFSRLGSHEFLQTAEVMRSSGTNASKRVAVNIID 60

Query: 61  ----------------LLGALKPGIAKRSVGYVCDSPKAALGANGMAVLSRRQDARGSVV 120
                           LLG LK GI+K+S  YVCD PKAA+G  G+ VLSR Q+ R SVV
Sbjct: 61  EAKRGLPDTYATSVRELLGPLKFGISKQSAVYVCDIPKAAIG--GIPVLSRWQNVRASVV 120

Query: 121 NFSTLVVTSRCDTTAGVPCFARFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEK 180
           NFSTL VTSRCDT+A  PCFARFMSSKSSE++GQ+ S+SKKEISTVEDPFDAPTYNIPEK
Sbjct: 121 NFSTLGVTSRCDTSARGPCFARFMSSKSSEKRGQTESESKKEISTVEDPFDAPTYNIPEK 180

Query: 181 PVTFAEGASYSFIILTGLGVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKR 240
           PVTFAEGASYSFIIL GLGVAAAAGYA               ++      YKIFDKALKR
Sbjct: 181 PVTFAEGASYSFIILAGLGVAAAAGYAVF------------KELIFQPKEYKIFDKALKR 240

Query: 241 IQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKV 300
           IQDDSQV+VRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHG GKV
Sbjct: 241 IQDDSQVRVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGAGKV 300

[Close]
BLAST of Carg01659 vs. TrEMBL
Match: tr|A0A1Q3C0P6|A0A1Q3C0P6_CEPFO (TIM21 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_17184 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.5e-75
Identity = 161/260 (61.92%), Postives = 194/260 (74.62%), Query Frame = 0 

Query: 43  LKPGIAKR-SVGYVCDSPKAALGANGMAVLSRRQDARGSVVNFST-LVVTSRCDTTAGVP 102
           LK G A+      V  + +  LGANG+ V+ R +  R S+V+FS+        +  A  P
Sbjct: 59  LKSGCARHIFTDAVLMTKQDVLGANGIPVIYRGRRTRESIVSFSSPFGRMENHEVKAMTP 118

Query: 103 CFARFMSSKSSEQQGQSSSQSKKEISTVEDPFDAPTYNIPEKPVTFAEGASYSFIILTGL 162
           C AR  +S++S+Q GQ+SS++KK+ISTVEDPFDAPTYNIP+KPVTFAEGASYS IIL GL
Sbjct: 119 CSARSFASRASKQPGQTSSETKKDISTVEDPFDAPTYNIPDKPVTFAEGASYSVIILAGL 178

Query: 163 GVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKALKRIQDDSQVKVRIGSPITGY 222
           GVAA A YA      ++  L+ +         YKIF KALKRIQDD QV+VRIGSPITGY
Sbjct: 179 GVAAVAAYA------VFKELIFEPK------EYKIFSKALKRIQDDGQVRVRIGSPITGY 238

Query: 223 GQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGGKVYTEMFKDQVEKQWKFTYL 282
           GQE+RNRAARQRIP+RV+TDEDGVERVEVNF++RGPHG GKVYTEMFKD+ +KQWKFTYL
Sbjct: 239 GQESRNRAARQRIPSRVYTDEDGVERVEVNFHVRGPHGAGKVYTEMFKDKADKQWKFTYL 298

Query: 283 IVEVKSSPPVQLILESYMPA 301
           IVE+ +    QLILESYMPA
Sbjct: 299 IVEITTPTHAQLILESYMPA 306

[Close]
BLAST of Carg01659 vs. TrEMBL
Match: tr|A0A2I4H1F3|A0A2I4H1F3_9ROSI (Ribosomal protein L15 OS=Juglans regia OX=51240 GN=LOC109012668 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.9e-74
Identity = 165/280 (58.93%), Postives = 188/280 (67.14%), Query Frame = 0 

Query: 30  RLGVQEYLQLLGALKPGIAKRSVGYVCDSPKAALGANGMAVLSRRQDARGSVVNFSTLVV 89
           RLG  E  QLL  LK G  ++   +  D  K  L ANG+  LS         +       
Sbjct: 43  RLG-DEANQLLVLLKSGCERQHTLHTNDMIKDVLRANGIPALS-------DCIYRYPFHF 102

Query: 90  TSRCDTTAGVPCFARFMSSKSSEQQGQSSSQ---------SKKEISTVEDPFDAPTYNIP 149
              C T   +P F R  SSK+SE+  Q+ S          SKKEISTVEDPFDAPTYNIP
Sbjct: 103 KQTCGTNTMIPFFTRSFSSKASERPRQTGSNKXXXXXXXXSKKEISTVEDPFDAPTYNIP 162

Query: 150 EKPVTFAEGASYSFIILTGLGVAAAAGYANHPSNSLWFPLLGDSDMQICLLRYKIFDKAL 209
           +KPVTF EGASY+ IIL GLGVAAAAGYA               ++      YK+F+KAL
Sbjct: 163 QKPVTFVEGASYTAIILAGLGVAAAAGYAVF------------KELIFQPKEYKVFNKAL 222

Query: 210 KRIQDDSQVKVRIGSPITGYGQETRNRAARQRIPNRVWTDEDGVERVEVNFYIRGPHGGG 269
           KRIQDDSQV+VRIGSPITGYGQE+RNRAARQRIPN++WTDEDGVE VEVNFYIRGPHG G
Sbjct: 223 KRIQDDSQVRVRIGSPITGYGQESRNRAARQRIPNKIWTDEDGVEHVEVNFYIRGPHGAG 282

Query: 270 KVYTEMFKDQVEKQWKFTYLIVEVKSSPPVQLILESYMPA 301
           KVY EMFKDQ +KQWK+TYLIVE+KS  P Q+ILESYMPA
Sbjct: 283 KVYCEMFKDQADKQWKYTYLIVEIKSPSPSQMILESYMPA 302

The following BLAST results are available for this feature:
BLAST of Carg01659 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022939739.11.3e-14289.33probable mitochondrial import inner membrane translocase subunit TIM21 isoform X... [more]
XP_023550442.14.2e-14188.67probable mitochondrial import inner membrane translocase subunit TIM21 [Cucurbit... [more]
XP_022992662.13.7e-12175.61probable mitochondrial import inner membrane translocase subunit TIM21 isoform X... [more]
XP_022992663.14.5e-11988.17probable mitochondrial import inner membrane translocase subunit TIM21 isoform X... [more]
XP_022990405.11.0e-11870.33probable mitochondrial import inner membrane translocase subunit TIM21 [Cucurbit... [more]
BLAST of Carg01659 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G00026.18.1e-7055.64FUNCTIONS IN: molecular_function unknown[more]
BLAST of Carg01659 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q1G3L1|TIM21_ARATH1.5e-6855.64Probable mitochondrial import inner membrane translocase subunit TIM21 OS=Arabid... [more]
BLAST of Carg01659 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3C3T0|A0A1S3C3T0_CUCME3.1e-11668.93probable mitochondrial import inner membrane translocase subunit TIM21 isoform X... [more]
tr|A0A0A0K9E8|A0A0A0K9E8_CUCSA4.5e-11570.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G046410 PE=4 SV=1[more]
tr|A0A1S3C3P7|A0A1S3C3P7_CUCME5.0e-11468.64probable mitochondrial import inner membrane translocase subunit TIM21 isoform X... [more]
tr|A0A1Q3C0P6|A0A1Q3C0P6_CEPFO4.5e-7561.92TIM21 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_17... [more]
tr|A0A2I4H1F3|A0A2I4H1F3_9ROSI2.9e-7458.93Ribosomal protein L15 OS=Juglans regia OX=51240 GN=LOC109012668 PE=3 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0030150protein import into mitochondrial matrix
Vocabulary: Cellular Component
TermDefinition
GO:0005744mitochondrial inner membrane presequence translocase complex
Vocabulary: INTERPRO
TermDefinition
IPR038552Tim21_IMS_sf
IPR013261Tim21
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0005744 mitochondrial inner membrane presequence translocase complex
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01659-RACarg01659-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013261Mitochondrial import inner membrane translocase subunit Tim21PFAMPF08294TIM21coord: 150..287
e-value: 5.3E-16
score: 58.7
IPR013261Mitochondrial import inner membrane translocase subunit Tim21PANTHERPTHR13032FAMILY NOT NAMEDcoord: 193..300
coord: 97..169
IPR038552Tim21 IMS domain superfamilyGENE3DG3DSA:3.10.450.320coord: 192..299
e-value: 4.5E-12
score: 47.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..133