Carg01257 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg01257
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Serine hydrolase FSH
Location	Cucurbita_argyrosperma_scaffold_002 : 1809302 .. 1810478 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGAGAGGAAGTAAATGAAATGGAGAGTGGAGGGAAGAGAGAAGAGAGCAGAGAGCAGAGAGATTGAAGATTTTATGCCTTCATGGATTCAGAACCAGTGGGAGTTTTCTCAGAAAGTAGCTCACCAAATGGGATCCTTCCATTTTCGCTCACTTTGATATGGTATTTTCCTTTTTTTATTTACTCATTATTAAAACATTATTTTATATAATTTTCTAATTATATAATAATTTTTGTATATTGAAATAGGAATTTCCAGATGCTATATTTCCAGCAGGAGGAAAATCAGAGATTGAAGGATATTTTCCACCTCCTTATTTTGAATGGTTTCAATTTAATGAGGTACACAATTAATTTAAAATATTTTTTAAAATTTAAATATATATATATATATTTATTTATTTATTTGACCTTTACCTGGCAGGATTTCACAGAGTACACAAACTTGGAACAATGCATTGATTACTTGAACAACTACATCACATCCAATGGCCCATTTCATGGACTCTTAGGCTTTTCTCAGGTTGCTTTATTTACCTTAATTTTACTCTTAATCATAATTTAATTCATTTTAATTTTTTAATCTTTTATCCAGGGCGCCGTATTGTCAGCACTCTTGTTGGGTTACCAAGCACAGGTACGTCTTAATTAACGCCAACAAATCTAGCATACCTGAAATGATTTTACCGCCAAAATGAACCTAACTACTATAATCGATACCGATAATGAACATAACTCAATATATCAAATTCAGTCAAAATCAATAAACAACACTAAATTTACGATCATTTTTTACCCATATCGTAACATCGTAAGCAGTTCAAAAATAACGAAACAGGGGAAGGTGTTGAAGGAGCATCCAGCAATGAAGATGTTTGTGTCAATATCAGGAACAAAGTTCAAAGATGCAGTTCAAAGATGAAAGCATCTGTGAGGTTGCACAAAATAAAGGTCAAATCTGTTCACCTGATAGGAGAAAAGGATTGGTTGAAGCTTCCTTCTGAAGAACTTGCTTCTGCCTTTCATGAGCCTCTCATCATAAGGCATCCTCAAGGTCATACTGTCCCTAGATTAGACGAAGTAGCCACTAAGAAGCTCCGCTGCTGGGTCGATGCGATAGTTTCGAGTCATGATCATGTTGGAGATGGAGAAGCAAAGGAGAATATCACTCAGAAA

mRNA sequence

GAGGAGAGGAAGTAAATGAAATGGAGAGTGGAGGGAAGAGAGAAGAGAGCAGAGAGCAGAGAGATTGAAGATTTTATGCCTTCATGGATTCAGAACCAGTGGGAGTTTTCTCAGAAAGTAGCTCACCAAATGGGATCCTTCCATTTTCGCTCACTTTGATATGGGCGCCGTATTGTCAGCACTCTTGTTGGGTTACCAAGCACAGGAACAAAGTTCAAAGATGCAGTTCAAAGATGAAAGCATCTGTGAGGTTGCACAAAATAAAGGTCAAATCTGTTCACCTGATAGGAGAAAAGGATTGGTTGAAGCTTCCTTCTGAAGAACTTGCTTCTGCCTTTCATGAGCCTCTCATCATAAGGCATCCTCAAGGTCATACTGTCCCTAGATTAGACGAAGTAGCCACTAAGAAGCTCCGCTGCTGGGTCGATGCGATAGTTTCGAGTCATGATCATGTTGGAGATGGAGAAGCAAAGGAGAATATCACTCAGAAA

Coding sequence (CDS)

ATGGATTCAGAACCAGTGGGAGTTTTCTCAGAAAGTAGCTCACCAAATGGGATCCTTCCATTTTCGCTCACTTTGATATGGGCGCCGTATTGTCAGCACTCTTGTTGGGTTACCAAGCACAGGAACAAAGTTCAAAGATGCAGTTCAAAGATGAAAGCATCTGTGAGGTTGCACAAAATAAAGGTCAAATCTGTTCACCTGATAGGAGAAAAGGATTGGTTGAAGCTTCCTTCTGAAGAACTTGCTTCTGCCTTTCATGAGCCTCTCATCATAAGGCATCCTCAAGGTCATACTGTCCCTAGATTAGACGAAGTAGCCACTAAGAAGCTCCGCTGCTGGGTCGATGCGATAGTTTCGAGTCATGATCATGTTGGAGATGGAGAAGCAAAGGAGAATATCACTCAGAAA

Protein sequence

MDSEPVGVFSESSSPNGILPFSLTLIWAPYCQHSCWVTKHRNKVQRCSSKMKASVRLHKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAIVSSHDHVGDGEAKENITQK

Homology

[Close]

BLAST of Carg01257 vs. NCBI nr
Match: XP_023539260.1 (family of serine hydrolases 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164.1 bits (414), Expect = 3.4e-37
Identity = 76/79 (96.20%), Postives = 78/79 (98.73%), Query Frame = 0


Query: 58  HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAI 117
           HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVAT++LRCWVDAI
Sbjct: 154 HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATEELRCWVDAI 213

Query: 118 VSSHDHVGDGEAKENITQK 137
           VSSHDHVGDGE KENITQK
Sbjct: 214 VSSHDHVGDGEVKENITQK 232

[Close]

BLAST of Carg01257 vs. NCBI nr
Match: XP_022971394.1 (uncharacterized protein LOC111470131 [Cucurbita maxima])

HSP 1 Score: 158.7 bits (400), Expect = 1.4e-35
Identity = 74/79 (93.67%), Postives = 77/79 (97.47%), Query Frame = 0


Query: 58  HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAI 117
           HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVAT++LRCWVDAI
Sbjct: 58  HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATEELRCWVDAI 117

Query: 118 VSSHDHVGDGEAKENITQK 137
           VSSH+ VGD EAKENITQK
Sbjct: 118 VSSHERVGDAEAKENITQK 136

[Close]

BLAST of Carg01257 vs. NCBI nr
Match: XP_004136600.1 (PREDICTED: ovarian cancer-associated gene 2 protein homolog [Cucumis sativus] >KGN59361.1 hypothetical protein Csa_3G813300 [Cucumis sativus])

HSP 1 Score: 145.2 bits (365), Expect = 1.6e-31
Identity = 65/79 (82.28%), Postives = 71/79 (89.87%), Query Frame = 0


Query: 58  HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAI 117
           HKIKVKSVH IGEKDWLKLPS++LAS FHEPLIIRHPQGHTVPRLDE ATK+L+CWVDAI
Sbjct: 156 HKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAI 215

Query: 118 VSSHDHVGDGEAKENITQK 137
           +SS DH GDGE KEN+  K
Sbjct: 216 LSSRDHDGDGEVKENVIDK 234

[Close]

BLAST of Carg01257 vs. NCBI nr
Match: XP_022154224.1 (dihydrofolate reductase-like [Momordica charantia])

HSP 1 Score: 135.6 bits (340), Expect = 1.3e-28
Identity = 62/76 (81.58%), Postives = 69/76 (90.79%), Query Frame = 0


Query: 58  HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAI 117
           HKI+ KSVHLIGEKDWLKLPS+ELASAFH+PLIIRHPQGHTVPRLDE ATK+L+CWVDA+
Sbjct: 153 HKIEAKSVHLIGEKDWLKLPSQELASAFHDPLIIRHPQGHTVPRLDEAATKELQCWVDAV 212

Query: 118 VSSHDHV-GDGEAKEN 133
           +S HDHV  D E KEN
Sbjct: 213 LSGHDHVIEDEEVKEN 228

[Close]

BLAST of Carg01257 vs. NCBI nr
Match: XP_008443142.1 (PREDICTED: esterase C25G4.2-like [Cucumis melo])

HSP 1 Score: 135.2 bits (339), Expect = 1.7e-28
Identity = 60/79 (75.95%), Postives = 68/79 (86.08%), Query Frame = 0


Query: 58  HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAI 117
           HKIKVKSVH IGEKDWLKLPS++LAS FH+PLIIRHPQGHTVPRLDE ATK+L+CWVD I
Sbjct: 154 HKIKVKSVHFIGEKDWLKLPSQQLASVFHQPLIIRHPQGHTVPRLDESATKELQCWVDTI 213

Query: 118 VSSHDHVGDGEAKENITQK 137
           +SS  H GD E KE++  K
Sbjct: 214 LSSRGHDGDSEVKESVIDK 232

[Close]

BLAST of Carg01257 vs. TAIR10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process)

HSP 1 Score: 61.6 bits (148), Expect = 4.3e-10
Identity = 24/70 (34.29%), Postives = 45/70 (64.29%), Query Frame = 0


Query: 48  SSKMKASVRLHKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVAT 107
           S+K+  +     ++  S+H +GE D+LK    +L  ++  P+++ HP+GHTVPRLDE + 
Sbjct: 145 SAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIESYKNPVVVHHPKGHTVPRLDEKSL 204

Query: 108 KKLRCWVDAI 118
           +K+  ++D +
Sbjct: 205 EKVTAFIDTL 214

[Close]

BLAST of Carg01257 vs. TAIR10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 54.7 bits (130), Expect = 5.2e-08
Identity = 22/58 (37.93%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 60  IKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAI 118
           ++  S+H IGE+D+LK+  E L  +F EP++I H  GH +P+LD  A + +  +  +I
Sbjct: 180 VRCPSLHFIGERDFLKIEGEVLVESFVEPVVIHHSGGHIIPKLDTKAEETMLSFFQSI 237

[Close]

BLAST of Carg01257 vs. TrEMBL
Match: tr|A0A0A0LH35|A0A0A0LH35_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G813300 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.1e-31
Identity = 65/79 (82.28%), Postives = 71/79 (89.87%), Query Frame = 0


Query: 58  HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAI 117
           HKIKVKSVH IGEKDWLKLPS++LAS FHEPLIIRHPQGHTVPRLDE ATK+L+CWVDAI
Sbjct: 156 HKIKVKSVHFIGEKDWLKLPSQQLASVFHEPLIIRHPQGHTVPRLDESATKELKCWVDAI 215

Query: 118 VSSHDHVGDGEAKENITQK 137
           +SS DH GDGE KEN+  K
Sbjct: 216 LSSRDHDGDGEVKENVIDK 234

[Close]

BLAST of Carg01257 vs. TrEMBL
Match: tr|A0A1S3B6W5|A0A1S3B6W5_CUCME (esterase C25G4.2-like OS=Cucumis melo OX=3656 GN=LOC103486820 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-28
Identity = 60/79 (75.95%), Postives = 68/79 (86.08%), Query Frame = 0


Query: 58  HKIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAI 117
           HKIKVKSVH IGEKDWLKLPS++LAS FH+PLIIRHPQGHTVPRLDE ATK+L+CWVD I
Sbjct: 154 HKIKVKSVHFIGEKDWLKLPSQQLASVFHQPLIIRHPQGHTVPRLDESATKELQCWVDTI 213

Query: 118 VSSHDHVGDGEAKENITQK 137
           +SS  H GD E KE++  K
Sbjct: 214 LSSRGHDGDSEVKESVIDK 232

[Close]

BLAST of Carg01257 vs. TrEMBL
Match: tr|A0A2P6RH73|A0A2P6RH73_ROSCH (Putative dihydrofolate reductase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0495211 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.8e-21
Identity = 50/63 (79.37%), Postives = 56/63 (88.89%), Query Frame = 0


Query: 59  KIKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAIV 118
           +IKVKSVHLIG+KDWLKLPSEELAS FH PLIIRHPQGHTVPRLDEVAT++LR W   I+
Sbjct: 153 RIKVKSVHLIGDKDWLKLPSEELASEFHNPLIIRHPQGHTVPRLDEVATEQLREWTKTII 212

Query: 119 SSH 122
            S+
Sbjct: 213 QSN 215

[Close]

BLAST of Carg01257 vs. TrEMBL
Match: tr|A0A1S3E1A1|A0A1S3E1A1_CICAR (dihydrofolate reductase-like OS=Cicer arietinum OX=3827 GN=LOC101515745 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 3.2e-20
Identity = 49/60 (81.67%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 60  IKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAIVS 119
           IK KSVH IGEKDWLK+PSEELASAFH+PLIIRHPQGHTVPRLDEVAT +L+ WV  I+S
Sbjct: 154 IKAKSVHFIGEKDWLKIPSEELASAFHKPLIIRHPQGHTVPRLDEVATGQLQNWVAEIIS 213

[Close]

BLAST of Carg01257 vs. TrEMBL
Match: tr|A0A2N9G3L3|A0A2N9G3L3_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS22030 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 4.2e-20
Identity = 53/84 (63.10%), Postives = 62/84 (73.81%), Query Frame = 0


Query: 60  IKVKSVHLIGEKDWLKLPSEELASAFHEPLIIRHPQGHTVPRLDEVATKKLRCWVDAIVS 119
           IKVKSVH IG KDWLKLPSEELA+AF  PLIIRHPQGHTVPRLDE AT++LR W   IV 
Sbjct: 154 IKVKSVHFIGAKDWLKLPSEELATAFDSPLIIRHPQGHTVPRLDEAATEQLRSWTAEIVK 213

Query: 120 SHDH-------VGDGEAKENITQK 137
            +++       + +GEAK  I +K
Sbjct: 214 CNNYAVEKKHELENGEAKVEIEEK 237

The following BLAST results are available for this feature:

BLAST of Carg01257 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023539260.1	3.4e-37	96.20	family of serine hydrolases 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022971394.1	1.4e-35	93.67	uncharacterized protein LOC111470131 [Cucurbita maxima]	[more]
XP_004136600.1	1.6e-31	82.28	PREDICTED: ovarian cancer-associated gene 2 protein homolog [Cucumis sativus] >K...	[more]
XP_022154224.1	1.3e-28	81.58	dihydrofolate reductase-like [Momordica charantia]	[more]
XP_008443142.1	1.7e-28	75.95	PREDICTED: esterase C25G4.2-like [Cucumis melo]	[more]

BLAST of Carg01257 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G24380.1	4.3e-10	34.29	INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri...	[more]
AT5G65400.1	5.2e-08	37.93	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Carg01257 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Carg01257 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LH35\|A0A0A0LH35_CUCSA	1.1e-31	82.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G813300 PE=4 SV=1	[more]
tr\|A0A1S3B6W5\|A0A1S3B6W5_CUCME	1.1e-28	75.95	esterase C25G4.2-like OS=Cucumis melo OX=3656 GN=LOC103486820 PE=4 SV=1	[more]
tr\|A0A2P6RH73\|A0A2P6RH73_ROSCH	3.8e-21	79.37	Putative dihydrofolate reductase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g049...	[more]
tr\|A0A1S3E1A1\|A0A1S3E1A1_CICAR	3.2e-20	81.67	dihydrofolate reductase-like OS=Cicer arietinum OX=3827 GN=LOC101515745 PE=4 SV=...	[more]
tr\|A0A2N9G3L3\|A0A2N9G3L3_FAGSY	4.2e-20	63.10	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS22030 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR029058	AB_hydrolase
IPR005645	FSH_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01257-RA	Carg01257-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005645	Serine hydrolase FSH	PFAM	PF03959	FSH1	coord: 51..106 e-value: 5.8E-8 score: 32.6
IPR029058	Alpha/Beta hydrolase fold	GENE3D	G3DSA:3.40.50.1820		coord: 13..120 e-value: 1.8E-9 score: 39.4
None	No IPR available	PANTHER	PTHR22778:SF22	SUBFAMILY NOT NAMED	coord: 59..125
None	No IPR available	PANTHER	PTHR22778	OVARIAN CANCER GENE-2 PROTEIN-RELATED	coord: 59..125

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg01257	Cla017488	Watermelon (97103) v1	carwmB0148
Carg01257	Csa3G813300	Cucumber (Chinese Long) v2	carcuB0172
Carg01257	MELO3C009683	Melon (DHL92) v3.5.1	carmeB0163
Carg01257	ClCG10G017000	Watermelon (Charleston Gray)	carwcgB0150
Carg01257	CSPI03G37070	Wild cucumber (PI 183967)	carcpiB0178
Carg01257	Cucsa.337060	Cucumber (Gy14) v1	carcgyB0198
Carg01257	CmoCh06G000590	Cucurbita moschata (Rifu)	carcmoB0227
Carg01257	Lsi03G016830	Bottle gourd (USVL1VR-Ls)	carlsiB143
Carg01257	CsGy3G034530	Cucumber (Gy14) v2	carcgybB0167
Carg01257	MELO3C009683.2	Melon (DHL92) v3.6.1	carmedB0164
Carg01257	CsaV3_3G039400	Cucumber (Chinese Long) v3	carcucB0178
Carg01257	Cla97C10G199850	Watermelon (97103) v2	carwmbB0162

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg01257	Silver-seed gourd	carcarB182
Carg01257	Cucurbita maxima (Rimu)	carcmaB0202
Carg01257	Cucurbita maxima (Rimu)	carcmaB0216
Carg01257	Cucurbita moschata (Rifu)	carcmoB0213
Carg01257	Cucurbita pepo (Zucchini)	carcpeB0229
Carg01257	Cucurbita pepo (Zucchini)	carcpeB0234
Carg01257	Wax gourd	carwgoB0197