Carg00906 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg00906
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Iron-sulfur cluster assembly
Location	Cucurbita_argyrosperma_scaffold_014 : 2066656 .. 2069321 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTCCGACTAGTAAAAGGTAGCTGTTCGCATATAAACCGTTCGCCGTTCGCCGGACTAATCGCGACCGGTTTTCTTCGATGGCGTTCTCTTCGATTACCGTGAAATGCCCCCCTGTTCTTCACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAGAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCGATTCGATCGGTTTCAGTCCCGGGTACGCCTCTTTATTTTTTCCCGCTGCTTTCCTCCTTGATTTTTGGGTATTATATTTGACTGTATCACCGTAGTTCATCGTTGTACTGTTTGGGGGAAAATTAATCAGGAATCTGTTTCTTTTGGATTGGAACTATGAACTTGGGAAGAACGTCAGGATTCGAGTTATTCTTTTAATTAATTAAACGTAGGATTCAATTTCTGGTTGCTTGGGATGGAAATTGTTGGCTCGTGGAAGTTTTATATTGAAATTAAGAAACAGAATTTTGGGGATTTTCTTATTGAGTTACACGGAAAATAAATTTCCCTGCTACTTGGAACGGATGATTTGTTTGTGAGTTCTAATTCGAGTTCCAGTTAGTGATATACAAACCATGAGTGTGCATATTTGTTTTGTATCAATGCCTAAATGATTAGGATGGATTGATCTTCATGTGGTTGCAAAGATGTTTACTAATTCTTGTGTTTGATAGCATTGATCTTTAATTCTTTTTTAGAGCATACAGAATGGATTTGAAATGATTAAACAACTCTTTTTCATGTTGAAACCACTCTGAAATATGTCTTAAATCAATTGAATGTTTGCTTTTATACTTTTAAATGCAATTTTCGTACTAGTTGAATGATTAAAAGTATGTTTTGGAGTGATTTTGAGTGTGAAAAAAAGTGATTTCAACCAATTTCAAAGTCACTCCTAAACATGACATGCAATATGGTTTTGTGAATGGCACTTTCCCTTTGCCGCTTACTAGAGATCAAAGATAAAATCTACTGAATCTTGCAGTTTCTGCATATGAAATATGATGAGTATATTATGCGTTATGATCACAGTTTTGCATTTTCATCTTATGTTGTTGAACATGTAAATTGTGATTAAACAGCTGCGCCAGCATCTGAAGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGGTTTGTGATTGGTAAGAGTTATAGCTTTTCTTTTTTCGCTCTGAATTTCTAGTGGGGTTCTTAGATTTATCCTTTTCGAATTCATTATTTTTGTAGATGACCCATTTCTATATATATACTCTTAGCTTCATGCCTTCAAAAGAACATGGTGGATCTAAGTTGAGTTTTGCATGTTTTTGGATCGATACTTTCTTTCCCATGGAATGGTAGAGGGAAAGTTTTCAATTCTTCTTAGGATGAAAGAAGCTTACACTTTTATATATTCGGATATAGATGGGCATTTCATGAGCCATATGCCATAAATTTGAAGAGCCCAAGCCCACCGCTAACAAATATTGTCCGCTTTGGGCCGTTACGTATCATCGTCAGCCTTACTGTTTTAACATGCGACTATTAGAGAGATGTTTTCACACTCTTATAAGTAATGCTTCGTTCTCCTCTTCAATCATTGTGGGATCCCACAATCCACCCCCCTTGGGGGCCCAACGTCCTCGCTGGTACACCGCTCAGTATTTGTCTCTGATAGCATTTGTAATAACTAGTCCACCGCTAGTAGATATTGTTTGCTTTGGATTGAGAGATCCCACATCGGTTAGAGAGGAGAATCAAACATTTCTTATAAGAGCGTGGAAACCCTCTCCCTAATAGACGCGTGTTAAAAACGTGAAACTGACGTCGATACGTAACGGGCCAAATCGGACAATATTTGCTAGCAGTGAGCTTGGACTAATAATTTTACTCAATAGTTCATAGTCTATCTCCCCTTTGGACACTCGATGACTTGAATGAATTGATATCATAATTTTTTTATGCTGACTGATAAGCAGGTTGTGCAACTTTTGTCAAATGTACTTTTCTTTAATGCATACTATTTCAACACGAGCTTGTATATTGTTTCGATTCCTTGACAAATGCTCTCCTACTCTATTGAATAAGGAACCTAAGCATCTTTCAGTGAACTATGAAGGTATCATTTTATCTACTTCATTTTCGACTGTGCCCTCTTAAGTCGTAGGCTCGACGATGATTACGAGTTCACTTATATCACTTGCTTTATAGCTTAAGCTTTTGATACAAACTTCCCTCTCTACTTGTTTTGTAGTATGCGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTCATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAATCATTTGCTGCAGAAATGTAGGATCATCATTTGTGTATATATATATATATATATATGAATGATCAACACAAACTTGATAGTTTCATATTAAAGAAAACTTAGAACGTGTAAAAACTCT

mRNA sequence

ATTTCCGACTAGTAAAAGGTAGCTGTTCGCATATAAACCGTTCGCCGTTCGCCGGACTAATCGCGACCGGTTTTCTTCGATGGCGTTCTCTTCGATTACCGTGAAATGCCCCCCTGTTCTTCACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAGAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCGATTCGATCGGTTTCAGTCCCGGCTGCGCCAGCATCTGAAGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGGTTTGTGATTGTATGCGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTCATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAATCATTTGCTGCAGAAATGTAGGATCATCATTTGTGTATATATATATATATATATATGAATGATCAACACAAACTTGATAGTTTCATATTAAAGAAAACTTAGAACGTGTAAAAACTCT

Coding sequence (CDS)

ATGGCGTTCTCTTCGATTACCGTGAAATGCCCCCCTGTTCTTCACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAGAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCGATTCGATCGGTTTCAGTCCCGGCTGCGCCAGCATCTGAAGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGGTTTGTGATTGTATGCGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTCATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAATCATTTGCTGCAGAAATGTAG

Protein sequence

MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM

Homology

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BLAST of Carg00906 vs. NCBI nr
Match: XP_022950286.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_023544833.1 iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 344.4 bits (882), Expect = 2.3e-91
Identity = 171/172 (99.42%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSIT+KCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

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BLAST of Carg00906 vs. NCBI nr
Match: XP_022977954.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 339.3 bits (869), Expect = 7.4e-90
Identity = 169/172 (98.26%), Postives = 170/172 (98.84%), Query Frame = 0


Query: 1   MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSIT+KCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

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BLAST of Carg00906 vs. NCBI nr
Match: XP_008440129.1 (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo])

HSP 1 Score: 328.2 bits (840), Expect = 1.7e-86
Identity = 164/172 (95.35%), Postives = 168/172 (97.67%), Query Frame = 0


Query: 1   MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSIT+K PPVLHLRALPKSSAPQSSISFR+SPTKFSR K+LSIRSVSVPAAPASE I
Sbjct: 1   MAFSSITMKFPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASESI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

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BLAST of Carg00906 vs. NCBI nr
Match: XP_022132407.1 (iron-sulfur assembly protein IscA, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 318.9 bits (816), Expect = 1.0e-83
Identity = 159/172 (92.44%), Postives = 165/172 (95.93%), Query Frame = 0


Query: 1   MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSS T+K P VLHLRALP+SSA QSSISFRYS TKFSRRK+LS+RSVSVPAAP SEGI
Sbjct: 1   MAFSSATMKFPSVLHLRALPESSATQSSISFRYSLTKFSRRKSLSVRSVSVPAAPTSEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNAL HLNKMRS+RNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALNHLNKMRSDRNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAA+M
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAADM 172

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BLAST of Carg00906 vs. NCBI nr
Match: XP_004141965.1 (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis sativus] >KGN48422.1 hypothetical protein Csa_6G486950 [Cucumis sativus])

HSP 1 Score: 317.0 bits (811), Expect = 3.9e-83
Identity = 160/174 (91.95%), Postives = 166/174 (95.40%), Query Frame = 0


Query: 1   MAFSSITVK--CPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60
           MAFSSI++K  CPPVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

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BLAST of Carg00906 vs. TAIR10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein)

HSP 1 Score: 244.2 bits (622), Expect = 5.9e-65
Identity = 120/149 (80.54%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 27  SSISF---RYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNE 86
           +SISF    Y     +RR  LS+RS SVPAAPA EG+ PAISL++NALKHL+KMRSER E
Sbjct: 32  NSISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGE 91

Query: 87  DLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 146
           DLCLRIGV+QGGCSGMSYTM+FENRANARPDDS IEY GF IVCDPKS+LFLFGMQLDYS
Sbjct: 92  DLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYS 151

Query: 147 DALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           DALIGGGFSF NPNATQTCGCGKSFAAEM
Sbjct: 152 DALIGGGFSFSNPNATQTCGCGKSFAAEM 180

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BLAST of Carg00906 vs. TAIR10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 81.6 bits (200), Expect = 5.0e-16
Identity = 41/110 (37.27%), Postives = 59/110 (53.64%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGF 123
           + L+DN ++ + +++S   E   LR+GV  GGCSG  Y  E +NR N  PDD + E NG 
Sbjct: 51  VHLSDNCIRRMKELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTN--PDDRVFEKNGV 110

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFK-NPNATQTCGCGKSFAAEM 173
            +V D  S   + G  +DY + LI   F    NP+A   C C  SF  ++
Sbjct: 111 KLVVDNVSYDLVKGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVKL 158

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BLAST of Carg00906 vs. TAIR10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 70.5 bits (171), Expect = 1.2e-12
Identity = 34/105 (32.38%), Postives = 60/105 (57.14%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGF 123
           ++L+D A   + ++   + +   LR+ V   GC+G+SY + +         D ++E  G 
Sbjct: 6   LALSDTAAARIRQLLQHQQKPF-LRLAVEAKGCNGLSYVLNYAQEKG--KFDEVVEEKGV 65

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 169
            I+ DPK+++ + G ++D+ D  +   F F NPNAT+ CGCG+SF
Sbjct: 66  KILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNATK-CGCGESF 106

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BLAST of Carg00906 vs. Swiss-Prot
Match: sp|Q9XIK3|ISCAP_ARATH (Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISCA PE=2 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-63
Identity = 120/149 (80.54%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 27  SSISF---RYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNE 86
           +SISF    Y     +RR  LS+RS SVPAAPA EG+ PAISL++NALKHL+KMRSER E
Sbjct: 32  NSISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGE 91

Query: 87  DLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 146
           DLCLRIGV+QGGCSGMSYTM+FENRANARPDDS IEY GF IVCDPKS+LFLFGMQLDYS
Sbjct: 92  DLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYS 151

Query: 147 DALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           DALIGGGFSF NPNATQTCGCGKSFAAEM
Sbjct: 152 DALIGGGFSFSNPNATQTCGCGKSFAAEM 180

[Close]

BLAST of Carg00906 vs. Swiss-Prot
Match: sp|P72731|Y1417_SYNY3 (Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1417 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.2e-36
Identity = 68/108 (62.96%), Postives = 83/108 (76.85%), Query Frame = 0


Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           A  I L+D ALKHL  ++ ++ +DLCLR+GVRQGGCSGMSY M+FE    A   D + +Y
Sbjct: 9   AKGIQLSDAALKHLLALKEQQGKDLCLRVGVRQGGCSGMSYMMDFEEPNRATEHDEVFDY 68

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 169
            GF I+CD KSLL+L+G+ LDYS+ALIGGGF F NPNA QTCGCGKSF
Sbjct: 69  EGFQIICDRKSLLYLYGLMLDYSNALIGGGFQFTNPNANQTCGCGKSF 116

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BLAST of Carg00906 vs. Swiss-Prot
Match: sp|P51217|YCF83_PORPU (Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.7e-32
Identity = 59/106 (55.66%), Postives = 83/106 (78.30%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGF 123
           I+LT+ A + +  ++S  + ++CLRIGV+QGGCSGMSY+M FE+ ++ R  D ++  + F
Sbjct: 8   ITLTEPAARQIKILKSNFDNEVCLRIGVKQGGCSGMSYSMNFESVSSLRETDEVLMLDSF 67

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           ++ CDPKSLL+L+G+ LDYS  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 68  LVACDPKSLLYLYGLSLDYSSELIGGGFQFSNPNASQTCGCGKSFS 113

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BLAST of Carg00906 vs. Swiss-Prot
Match: sp|Q1XDR9|YCF83_PYRYE (Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.5e-30
Identity = 61/112 (54.46%), Postives = 81/112 (72.32%), Query Frame = 0


Query: 58  EGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSI 117
           EG    I++T  ALK +  ++++   D+ LRIGVRQGGCSGMSY+M FE+    +  D  
Sbjct: 2   EGNMGFITITKPALKQIAILKNDHENDVHLRIGVRQGGCSGMSYSMNFEHVDKLKDTDER 61

Query: 118 IEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           +  + F +VCDPKSLL+L+G+ LD+S  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 62  LRLDNFSVVCDPKSLLYLYGLSLDFSSELIGGGFQFSNPNASQTCGCGKSFS 113

[Close]

BLAST of Carg00906 vs. Swiss-Prot
Match: sp|Q9MSA1|YCF83_GALSU (Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 7.7e-30
Identity = 58/107 (54.21%), Postives = 81/107 (75.70%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNG 123
           I++T +AL HLN +    +ED L +RIG++QGGCSG+SY M +E ++N   +D + +Y+ 
Sbjct: 6   INITKSALDHLNSISKLNSEDKLYIRIGIKQGGCSGLSYFMTYEKKSNISDNDLVYDYDN 65

Query: 124 FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           F +VCD KS+L+L+G+ LDYS +LI GGF F NPNA QTCGCGKSF+
Sbjct: 66  FTLVCDNKSILYLYGISLDYSSSLIDGGFKFLNPNAKQTCGCGKSFS 112

[Close]

BLAST of Carg00906 vs. TrEMBL
Match: tr|A0A1S3B105|A0A1S3B105_CUCME (iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484688 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.1e-86
Identity = 164/172 (95.35%), Postives = 168/172 (97.67%), Query Frame = 0


Query: 1   MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSIT+K PPVLHLRALPKSSAPQSSISFR+SPTKFSR K+LSIRSVSVPAAPASE I
Sbjct: 1   MAFSSITMKFPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASESI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]

BLAST of Carg00906 vs. TrEMBL
Match: tr|A0A0A0KJ66|A0A0A0KJ66_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.6e-83
Identity = 160/174 (91.95%), Postives = 166/174 (95.40%), Query Frame = 0


Query: 1   MAFSSITVK--CPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60
           MAFSSI++K  CPPVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

[Close]

BLAST of Carg00906 vs. TrEMBL
Match: tr|A0A2I4EFK0|A0A2I4EFK0_9ROSI (iron-sulfur assembly protein IscA, chloroplastic-like OS=Juglans regia OX=51240 GN=LOC108989112 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-73
Identity = 142/173 (82.08%), Postives = 156/173 (90.17%), Query Frame = 0


Query: 1   MAFSSITVKCPPVLHL-RALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEG 60
           MAFS+I+ +CP  L L +  P SS+PQ+S+SFR+S +K + RK LSIR+VSV AAP SEG
Sbjct: 1   MAFSAISTRCPFFLPLPQNPPSSSSPQNSVSFRFSQSKLNGRKPLSIRAVSVSAAPTSEG 60

Query: 61  IAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIE 120
             PAISLTDNALKHLNKMRS+RNEDLCLRIGV+QGGCSGMSYTMEFENRAN RPDDSIIE
Sbjct: 61  TTPAISLTDNALKHLNKMRSDRNEDLCLRIGVKQGGCSGMSYTMEFENRANKRPDDSIIE 120

Query: 121 YNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           YNGFVIVCDPKSLLF+FGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 YNGFVIVCDPKSLLFVFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173

[Close]

BLAST of Carg00906 vs. TrEMBL
Match: tr|A0A140B490|A0A140B490_PRUPE (Fe-S cluster assembly protein OS=Prunus persica var. persica OX=323852 GN=ISA2 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.0e-71
Identity = 137/173 (79.19%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 1   MAFSSITVKCPPVLHLRA-LPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEG 60
           MAFS+IT  CP +L     LP + +P +SISFR+S    SRRK LSIRS S  AAP +EG
Sbjct: 1   MAFSAITTHCPSLLRPPTNLPVTFSPPNSISFRFSKNNLSRRKPLSIRSTSTSAAPKTEG 60

Query: 61  IAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIE 120
           +APAISLTDNALKH NKMRSER EDLCLRIGV+QGGCSGMSYTM+FE+RANARPDDS+IE
Sbjct: 61  LAPAISLTDNALKHFNKMRSERGEDLCLRIGVKQGGCSGMSYTMDFESRANARPDDSVIE 120

Query: 121 YNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           YNGFV+VCDPKSLLFL+GMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 YNGFVMVCDPKSLLFLYGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173

[Close]

BLAST of Carg00906 vs. TrEMBL
Match: tr|M5WD48|M5WD48_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G169400 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.0e-71
Identity = 137/173 (79.19%), Postives = 151/173 (87.28%), Query Frame = 0


Query: 1   MAFSSITVKCPPVLHLRA-LPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEG 60
           MAFS+IT  CP +L     LP + +P +SISFR+S    SRRK LSIRS S  AAP +EG
Sbjct: 1   MAFSAITTHCPSLLRPPTNLPVTFSPPNSISFRFSKNNLSRRKPLSIRSTSTSAAPKTEG 60

Query: 61  IAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIE 120
           +APAISLTDNALKH NKMRSER EDLCLRIGV+QGGCSGMSYTM+FE+RANARPDDS+IE
Sbjct: 61  LAPAISLTDNALKHFNKMRSERGEDLCLRIGVKQGGCSGMSYTMDFESRANARPDDSVIE 120

Query: 121 YNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           YNGFV+VCDPKSLLFL+GMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 YNGFVMVCDPKSLLFLYGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 173

The following BLAST results are available for this feature:

BLAST of Carg00906 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950286.1	2.3e-91	99.42	iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_0...	[more]
XP_022977954.1	7.4e-90	98.26	iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima]	[more]
XP_008440129.1	1.7e-86	95.35	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo]	[more]
XP_022132407.1	1.0e-83	92.44	iron-sulfur assembly protein IscA, chloroplastic isoform X1 [Momordica charantia...	[more]
XP_004141965.1	3.9e-83	91.95	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis sativus] >K...	[more]

BLAST of Carg00906 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G10500.1	5.9e-65	80.54	chloroplast-localized ISCA-like protein	[more]
AT5G03905.1	5.0e-16	37.27	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	1.2e-12	32.38	Iron-sulphur cluster biosynthesis family protein	[more]

BLAST of Carg00906 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9XIK3\|ISCAP_ARATH	1.1e-63	80.54	Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
sp\|P72731\|Y1417_SYNY3	3.2e-36	62.96	Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ...	[more]
sp\|P51217\|YCF83_PORPU	3.7e-32	55.66	Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1	[more]
sp\|Q1XDR9\|YCF83_PYRYE	1.5e-30	54.46	Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1	[more]
sp\|Q9MSA1\|YCF83_GALSU	7.7e-30	54.21	Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 S...	[more]

BLAST of Carg00906 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3B105\|A0A1S3B105_CUCME	1.1e-86	95.35	iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1...	[more]
tr\|A0A0A0KJ66\|A0A0A0KJ66_CUCSA	2.6e-83	91.95	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950 PE=4 SV=1	[more]
tr\|A0A2I4EFK0\|A0A2I4EFK0_9ROSI	1.1e-73	82.08	iron-sulfur assembly protein IscA, chloroplastic-like OS=Juglans regia OX=51240 ...	[more]
tr\|A0A140B490\|A0A140B490_PRUPE	3.0e-71	79.19	Fe-S cluster assembly protein OS=Prunus persica var. persica OX=323852 GN=ISA2 P...	[more]
tr\|M5WD48\|M5WD48_PRUPE	3.0e-71	79.19	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G169400 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0097428	protein maturation by iron-sulfur cluster transfer

Vocabulary: **INTERPRO**
Term	Definition
IPR017870	FeS_cluster_insertion_CS
IPR000361	FeS_biogenesis
IPR035903	HesB-like_dom_sf
IPR016092	FeS_cluster_insertion

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00906-RA	Carg00906-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 64..169 e-value: 2.8E-37 score: 125.1
IPR035903	HesB-like domain superfamily	GENE3D	G3DSA:2.60.300.12		coord: 63..171 e-value: 1.1E-36 score: 127.1
IPR035903	HesB-like domain superfamily	SUPERFAMILY	SSF89360	HesB-like domain	coord: 63..159
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 64..166 e-value: 4.2E-22 score: 78.3
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 21..172
None	No IPR available	PANTHER	PTHR10072:SF48	IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTIC	coord: 21..172
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 151..168

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg00906	Cla008487	Watermelon (97103) v1	carwmB0405
Carg00906	Csa6G486950	Cucumber (Chinese Long) v2	carcuB0441
Carg00906	MELO3C007469	Melon (DHL92) v3.5.1	carmeB0414
Carg00906	ClCG01G015990	Watermelon (Charleston Gray)	carwcgB0397
Carg00906	CSPI06G27140	Wild cucumber (PI 183967)	carcpiB0446
Carg00906	CmaCh03G010480	Cucurbita maxima (Rimu)	carcmaB0567
Carg00906	CmaCh07G007610	Cucurbita maxima (Rimu)	carcmaB0571
Carg00906	CmoCh03G010460	Cucurbita moschata (Rifu)	carcmoB0551
Carg00906	CmoCh07G007820	Cucurbita moschata (Rifu)	carcmoB0554
Carg00906	Lsi01G010810	Bottle gourd (USVL1VR-Ls)	carlsiB364
Carg00906	Cp4.1LG19g11660	Cucurbita pepo (Zucchini)	carcpeB0563
Carg00906	Cp4.1LG10g03120	Cucurbita pepo (Zucchini)	carcpeB0554
Carg00906	CsGy6G026480	Cucumber (Gy14) v2	carcgybB0418
Carg00906	MELO3C007469.2	Melon (DHL92) v3.6.1	carmedB0418
Carg00906	CsaV3_6G042270	Cucumber (Chinese Long) v3	carcucB0442
Carg00906	Cla97C01G014980	Watermelon (97103) v2	carwmbB0426
Carg00906	Bhi03G001108	Wax gourd	carwgoB0492

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg00906	Carg05270	Silver-seed gourd	carcarB428

The following block(s) are covering this gene:


Gene	Organism	Block
Carg00906	Cucumber (Gy14) v1	carcgyB0467
Carg00906	Cucurbita maxima (Rimu)	carcmaB0565
Carg00906	Cucurbita pepo (Zucchini)	carcpeB0560
Carg00906	Melon (DHL92) v3.5.1	carmeB0403