Carg00547 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg00547
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	IST1-like protein
Location	Cucurbita_argyrosperma_scaffold_014 : 284850 .. 287559 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTCTTCTTCTTCTTCTTCTTCTTCTTCGTTACCCCTTTTTTTTCTCCGGCCATGAGCGCCGCCGATCGTTCCGTCAGGATCATCAAGAAGTTCATCTCCCTCCTCCGCCGTGGCTTCAACTCCTCCAAATGGTACTACGATTGTTTCTCAGAAATGGGTTTCGGATTTTTGTTTTAAAATGTGGGAAAATTGAGAGGAATAGGGATTGATTCGTTTGTTTTTTCTTCTGGATTTTGGGGGTTTTTCTTTTGTGACAGTAAAACGGCGGCGAAAATGGCGGTGGCGAGGATAAAGCTGCTGAGGAACAAGCGAGAGGCGGCGGTAAAGCAGATGAGACGCGACATTGCTCTGCTTCTTCAGTCGGGTCAGGACGCCACTGCTCGGATTCGGGTCATTCACTATCTTCGATTTCTTCGCCTTCGTTAAAAACGATTAAATTGAAATGTTTGAATCAAAGAAATGAAGGTTGTGATGTTCAGGTAGAACATGTGATAAGAGAACAGAATGTTTTGGCTGCCAATGAGATTATCGAACTCTTCTGCGAGTTGGTTGTGGCTAGATTATCAATTATAGCGAAACAGAGGTAGAAACGTTTAAGGAGAATGGGCTAAATTACTCTGTAATGGGTGGATTTTCTTGTGATGTTAATATCAATTGGTGCAGGGAATGTCCGGCAGATCTGAAAGAAGGGGTTGCTAGTTTGATCTTTGCAGCTCCGAGGTGCTCTGAGATTCCAGAGCTTTCTGCACTTACCAATGTTTTTGAGAAGAAATATGGGAAGGATTTTGTGTCTGCTGCCACTGATCTAAGGCCCAATTGTGGAGTGAATCGGCTGGTAACTACATTTTGGCCTTTTACTTATGGTCGGTTTTGGTAACACTAACGCCCAATTGGTTGGAGAGGAGAACAAAACACGACATTATAAGGGTGTCGAAACCTTTAGATGCATTTTAAAATCATTGAGTGAAAGCTCGAAAGGGAAAGCCCGAAGAGACAATGGTATCAGAGCTAGACACCGGACGATGTGTCAGCGAGGAGGCTGTTCTCCAAATGGGGTAGACACAAGGCCGTGCGCCAGTAAAGATGCTGGGGGCTCGAGGAGGTGGATTTGGTGGGGGTCCCACATCAATTGGAGAAGTGAATAAATGCCAGCGGGACAATGGGTCCTGAAGGAGGGTGAATTGTGATGTCCCACATCGGTTGGGGAGTAGAACGAAACACCCTTTGTAAGGGTGTGGAAACCTCTCCTTAGCAGATGCTTTTTAAAAGCTTTGAGGGGAAGCTTGAAAGGGAACGTCGGTGGGCTTGGGCTGTTACATGCTGGTCCTCAAGAGAATGGAGGGCTTGGACTGTTATATGTTGGCCCTCAAGGGGATGGATTGTGAGATCCTACGTTAGTTGGAAAGGGGTTTATACGGTAAACTATTGGGGGCTCATGTAGCCTACGCCGGATTAATCGTATTCTGGGTCGGAGCAATGAACGTATTCGAAGTGGCTCATTTCGTACCTGAGTAAAACGTGTTTTAAAACTGCGAGGGTGATAGTGATAAGTTAGTTATATTCATAGAGCTGTATTTGTATTGAATTGCTTGCGTTTGATTTTCCAGCTCATTGACAAGCTCTCTGTTCGAACTCCTGCGGGTGAAGTGAAGCTTAAAGTAATGAAGGAGATTGCCAAGGAGCACCAGATTGAATGGGATACGACAGAATCTGAGAAGGAGCTACTAAACCCTCCTGAAGAACTTATTGTCTGTTCCATTCTCCCTCTCTTCCTATCCTCTGATGATAATATTGTAATAAGTTACCGATGTTTTCGATTGTCGTTTGATCAGGACGGGCCTCGGAGTTTCGTTAGCGCTGCTAGCTTACCTGTGAAGCCTGCAGCAGCAGCAAGCCAATCTGCTGGAAATGATGCTCAAATTGAAAGGTAAGGAGGAAGGTACATTGGAACCATTTGATACACTCCATTGGAGCTGTTCCTCGACTCGATTCCATTGTCGTGTCTTGTGCAGGAGGAATGGTAGAGAGGATGAAAGTATGCACTTTCAAGATACAGCATCAGCCGCAGAAGCTGCTGCAAAAGCAGCGAAGCAAGCGATTGCTGCTGCAGAGGCAGCTGCCTATCTAGCAAATAAAGACATGAATTGGGATGCTCGAGACTCGGGTTTCGATCTCAGCCCTCCTGCTAATTCTACCCCGATCTGCTCTCATCATATTGATCATCAGTTCAAGGCAGGAGAAGAGAGAACAACGAATGTAAATACAGATTATGAGATGGCTTACAGGAGACATAGCTATAATCCTACCGACATAAAGTTCGACGAATCGGATTGTGAAGAAGAAACTCAAATGGACGACGGAGCTGACGGAGGTGATAGTCGACCTCCTGATGGGAATCCTCCACCGATACCTTCATCTCGTGTCCACCCCAAGCTGCCTGATTATGACACTCTTGCTGCTCGCTTTGAAGCTCTCAAGTACAGGAAAACTTGAACTTAGTCAGAATGTTGTTATAATTACTTGCCAATGATGCATGTAATTACTCAACCTTATTGCATTCAAATAAACCAGGAGTATTCTTTTGTTAGCTGTTTACAACACCCTATATAATCAAATACTGTAAAAAGATCAGAGATTATGGTATTCAGAATGCAATTCTTATACAATATTGGTTGAGATATATTGATGAAGTTTATCATGGTGTGTGTA

mRNA sequence

ACTCTTCTTCTTCTTCTTCTTCTTCTTCGTTACCCCTTTTTTTTCTCCGGCCATGAGCGCCGCCGATCGTTCCGTCAGGATCATCAAGAAGTTCATCTCCCTCCTCCGCCGTGGCTTCAACTCCTCCAAATGTAAAACGGCGGCGAAAATGGCGGTGGCGAGGATAAAGCTGCTGAGGAACAAGCGAGAGGCGGCGGTAAAGCAGATGAGACGCGACATTGCTCTGCTTCTTCAGTCGGGTCAGGACGCCACTGCTCGGATTCGGGTAGAACATGTGATAAGAGAACAGAATGTTTTGGCTGCCAATGAGATTATCGAACTCTTCTGCGAGTTGGTTGTGGCTAGATTATCAATTATAGCGAAACAGAGGGAATGTCCGGCAGATCTGAAAGAAGGGGTTGCTAGTTTGATCTTTGCAGCTCCGAGGTGCTCTGAGATTCCAGAGCTTTCTGCACTTACCAATCTCATTGACAAGCTCTCTGTTCGAACTCCTGCGGGTGAAGTGAAGCTTAAAGTAATGAAGGAGATTGCCAAGGAGCACCAGATTGAATGGGATACGACAGAATCTGAGAAGGAGCTACTAAACCCTCCTGAAGAACTTATTGTCTGTTCCATTCTCCCTCTCTTCCTATCCTCTGATGATAATATTGTAATAAGTTACCGATGTTTTCGATTGTCGTTTGATCAGGACGGGCCTCGGAGTTTCGTTAGCGCTGCTAGCTTACCTGTGAAGCCTGCAGCAGCAGCAAGCCAATCTGCTGGAAATGATGCTCAAATTGAAAGGAGGAATGGTAGAGAGGATGAAAGTATGCACTTTCAAGATACAGCATCAGCCGCAGAAGCTGCTGCAAAAGCAGCGAAGCAAGCGATTGCTGCTGCAGAGGCAGCTGCCTATCTAGCAAATAAAGACATGAATTGGGATGCTCGAGACTCGGGTTTCGATCTCAGCCCTCCTGCTAATTCTACCCCGATCTGCTCTCATCATATTGATCATCAGTTCAAGGCAGGAGAAGAGAGAACAACGAATGTAAATACAGATTATGAGATGGCTTACAGGAGACATAGCTATAATCCTACCGACATAAAGTTCGACGAATCGGATTGTGAAGAAGAAACTCAAATGGACGACGGAGCTGACGGAGGTGATAGTCGACCTCCTGATGGGAATCCTCCACCGATACCTTCATCTCGTGTCCACCCCAAGCTGCCTGATTATGACACTCTTGCTGCTCGCTTTGAAGCTCTCAAGTACAGGAAAACTTGAACTTAGTCAGAATGTTGTTATAATTACTTGCATTCAAATAAACCAGGAGTATTCTTTTGTTAGCTGTTTACAACACCCTATATAATCAAATACTGTAAAAAGATCAGAGATTATAATATTGGTTGAGATATATTGATGAAGTTTATCATGGTGTGTGTA

Coding sequence (CDS)

ATGAGCGCCGCCGATCGTTCCGTCAGGATCATCAAGAAGTTCATCTCCCTCCTCCGCCGTGGCTTCAACTCCTCCAAATGTAAAACGGCGGCGAAAATGGCGGTGGCGAGGATAAAGCTGCTGAGGAACAAGCGAGAGGCGGCGGTAAAGCAGATGAGACGCGACATTGCTCTGCTTCTTCAGTCGGGTCAGGACGCCACTGCTCGGATTCGGGTAGAACATGTGATAAGAGAACAGAATGTTTTGGCTGCCAATGAGATTATCGAACTCTTCTGCGAGTTGGTTGTGGCTAGATTATCAATTATAGCGAAACAGAGGGAATGTCCGGCAGATCTGAAAGAAGGGGTTGCTAGTTTGATCTTTGCAGCTCCGAGGTGCTCTGAGATTCCAGAGCTTTCTGCACTTACCAATCTCATTGACAAGCTCTCTGTTCGAACTCCTGCGGGTGAAGTGAAGCTTAAAGTAATGAAGGAGATTGCCAAGGAGCACCAGATTGAATGGGATACGACAGAATCTGAGAAGGAGCTACTAAACCCTCCTGAAGAACTTATTGTCTGTTCCATTCTCCCTCTCTTCCTATCCTCTGATGATAATATTGTAATAAGTTACCGATGTTTTCGATTGTCGTTTGATCAGGACGGGCCTCGGAGTTTCGTTAGCGCTGCTAGCTTACCTGTGAAGCCTGCAGCAGCAGCAAGCCAATCTGCTGGAAATGATGCTCAAATTGAAAGGAGGAATGGTAGAGAGGATGAAAGTATGCACTTTCAAGATACAGCATCAGCCGCAGAAGCTGCTGCAAAAGCAGCGAAGCAAGCGATTGCTGCTGCAGAGGCAGCTGCCTATCTAGCAAATAAAGACATGAATTGGGATGCTCGAGACTCGGGTTTCGATCTCAGCCCTCCTGCTAATTCTACCCCGATCTGCTCTCATCATATTGATCATCAGTTCAAGGCAGGAGAAGAGAGAACAACGAATGTAAATACAGATTATGAGATGGCTTACAGGAGACATAGCTATAATCCTACCGACATAAAGTTCGACGAATCGGATTGTGAAGAAGAAACTCAAATGGACGACGGAGCTGACGGAGGTGATAGTCGACCTCCTGATGGGAATCCTCCACCGATACCTTCATCTCGTGTCCACCCCAAGCTGCCTGATTATGACACTCTTGCTGCTCGCTTTGAAGCTCTCAAGTACAGGAAAACTTGA

Protein sequence

MSAADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSALTNLIDKLSVRTPAGEVKLKVMKEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDGPRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAAEAAAKAAKQAIAAAEAAAYLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAYRRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSSRVHPKLPDYDTLAARFEALKYRKT

Homology

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BLAST of Carg00547 vs. NCBI nr
Match: XP_022950275.1 (IST1-like protein [Cucurbita moschata])

HSP 1 Score: 645.6 bits (1664), Expect = 1.1e-181
Identity = 375/429 (87.41%), Postives = 375/429 (87.41%), Query Frame = 0


Query: 1   MSAADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL 60
           MSAADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL
Sbjct: 1   MSAADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL 60

Query: 61  QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI 120
           QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI
Sbjct: 61  QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI 120

Query: 121 FAAPRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLK 180
           FAAPRCSEIPELSALTN                          LIDKLSVRTPAGEVKLK
Sbjct: 121 FAAPRCSEIPELSALTNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPAGEVKLK 180

Query: 181 VMKEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDG 240
           VMKEIAKEHQIEWDTTESEKELLNPPEELI                            DG
Sbjct: 181 VMKEIAKEHQIEWDTTESEKELLNPPEELI----------------------------DG 240

Query: 241 PRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXX 300
           PRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXX
Sbjct: 241 PRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXX 300

Query: 301 XXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAY 360
           XXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAY
Sbjct: 301 XXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAY 360

Query: 361 RRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSSRVHPKLPDYDTLAAR 404
           RRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSSRVHPKLPDYDTLAAR
Sbjct: 361 RRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSSRVHPKLPDYDTLAAR 401

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BLAST of Carg00547 vs. NCBI nr
Match: XP_023544297.1 (IST1-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 631.7 bits (1628), Expect = 1.7e-177
Identity = 369/429 (86.01%), Postives = 371/429 (86.48%), Query Frame = 0


Query: 1   MSAADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL 60
           MSAADRSVRIIK+FISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL
Sbjct: 1   MSAADRSVRIIKQFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL 60

Query: 61  QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI 120
           QSGQDATARIRVEHVIREQNVLAANEIIELF ELVVARLSIIAKQRECPADLKEGVASLI
Sbjct: 61  QSGQDATARIRVEHVIREQNVLAANEIIELFSELVVARLSIIAKQRECPADLKEGVASLI 120

Query: 121 FAAPRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLK 180
           FAAPRCSEIPELSALTN                          LIDKLSVRTPAGEVKLK
Sbjct: 121 FAAPRCSEIPELSALTNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPAGEVKLK 180

Query: 181 VMKEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDG 240
           +MKEIA EHQIEWDTTESEKELL PPEELI                            DG
Sbjct: 181 IMKEIAMEHQIEWDTTESEKELLKPPEELI----------------------------DG 240

Query: 241 PRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXX 300
           PRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXX
Sbjct: 241 PRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXX 300

Query: 301 XXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAY 360
           XXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAY
Sbjct: 301 XXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAY 360

Query: 361 RRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSSRVHPKLPDYDTLAAR 404
           RRHSYNPTDIKFDESDCEEETQMDDGADGG SRPPDGNPPPIPSSRVHPKLPDYDTLAAR
Sbjct: 361 RRHSYNPTDIKFDESDCEEETQMDDGADGGVSRPPDGNPPPIPSSRVHPKLPDYDTLAAR 401

[Close]

BLAST of Carg00547 vs. NCBI nr
Match: XP_022977299.1 (IST1-like protein isoform X1 [Cucurbita maxima])

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 361/429 (84.15%), Postives = 367/429 (85.55%), Query Frame = 0


Query: 1   MSAADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL 60
           MSAADRSVRI+K+FISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL
Sbjct: 24  MSAADRSVRIVKQFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL 83

Query: 61  QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI 120
           QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI
Sbjct: 84  QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI 143

Query: 121 FAAPRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLK 180
           FAAPRCSEIPELSALTN                          LIDKLSVRTPAGEVKLK
Sbjct: 144 FAAPRCSEIPELSALTNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPAGEVKLK 203

Query: 181 VMKEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDG 240
           +MKEIAKEHQIEWDTTESEKELL PPE LI                            +G
Sbjct: 204 IMKEIAKEHQIEWDTTESEKELLKPPEGLI----------------------------EG 263

Query: 241 PRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXX 300
           PRSFV AASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQD ASA XXXXXXXXXXXX
Sbjct: 264 PRSFVCAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDKASAAXXXXXXXXXXXX 323

Query: 301 XXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAY 360
           XXXXXXX ANKDMNWDA +SGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDY+MAY
Sbjct: 324 XXXXXXXXANKDMNWDALNSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYDMAY 383

Query: 361 RRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSSRVHPKLPDYDTLAAR 404
           RRHSYNPTDIKFDESDCEEETQMDDGADGG SRPPDGNPPPIPSSRVHPKLPDYDTLAAR
Sbjct: 384 RRHSYNPTDIKFDESDCEEETQMDDGADGGVSRPPDGNPPPIPSSRVHPKLPDYDTLAAR 424

[Close]

BLAST of Carg00547 vs. NCBI nr
Match: XP_022977300.1 (IST1-like protein isoform X2 [Cucurbita maxima])

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 361/429 (84.15%), Postives = 367/429 (85.55%), Query Frame = 0


Query: 1   MSAADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL 60
           MSAADRSVRI+K+FISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL
Sbjct: 1   MSAADRSVRIVKQFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLL 60

Query: 61  QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI 120
           QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI
Sbjct: 61  QSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLI 120

Query: 121 FAAPRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLK 180
           FAAPRCSEIPELSALTN                          LIDKLSVRTPAGEVKLK
Sbjct: 121 FAAPRCSEIPELSALTNVFEKKYGKDFVSAATDLRPNCGVNRLLIDKLSVRTPAGEVKLK 180

Query: 181 VMKEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDG 240
           +MKEIAKEHQIEWDTTESEKELL PPE LI                            +G
Sbjct: 181 IMKEIAKEHQIEWDTTESEKELLKPPEGLI----------------------------EG 240

Query: 241 PRSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXX 300
           PRSFV AASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQD ASA XXXXXXXXXXXX
Sbjct: 241 PRSFVCAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDKASAAXXXXXXXXXXXX 300

Query: 301 XXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYEMAY 360
           XXXXXXX ANKDMNWDA +SGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDY+MAY
Sbjct: 301 XXXXXXXXANKDMNWDALNSGFDLSPPANSTPICSHHIDHQFKAGEERTTNVNTDYDMAY 360

Query: 361 RRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSSRVHPKLPDYDTLAAR 404
           RRHSYNPTDIKFDESDCEEETQMDDGADGG SRPPDGNPPPIPSSRVHPKLPDYDTLAAR
Sbjct: 361 RRHSYNPTDIKFDESDCEEETQMDDGADGGVSRPPDGNPPPIPSSRVHPKLPDYDTLAAR 401

[Close]

BLAST of Carg00547 vs. NCBI nr
Match: XP_004134834.1 (PREDICTED: IST1-like protein [Cucumis sativus] >KGN48972.1 hypothetical protein Csa_6G507450 [Cucumis sativus])

HSP 1 Score: 478.0 bits (1229), Expect = 3.1e-131
Identity = 307/448 (68.53%), Postives = 329/448 (73.44%), Query Frame = 0


Query: 3   AADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQS 62
           AA RS++I+K+FI++LR GFNSSKCKTAAKMAVARIKLLRNKREA VKQMRRDIALLLQS
Sbjct: 7   AAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQS 66

Query: 63  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA 122
           GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQR+CPADLKEGVASLIFA
Sbjct: 67  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFA 126

Query: 123 APRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLKVM 182
           APRCSEIPELSAL N                          LIDKLSVRTP GEVKLK+M
Sbjct: 127 APRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIM 186

Query: 183 KEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDGPR 242
           KEIAKEH+IEWDTTESEKELL P EELI                            +GPR
Sbjct: 187 KEIAKEHKIEWDTTESEKELLKPSEELI----------------------------EGPR 246

Query: 243 SFVSAASLPVKPAAAASQSAGNDAQIER-RNGREDESMHFQDTASAXXXXXXXXXXXXXX 302
           +FVSAASLPVKP  + S    ++AQIER  N RE+ESMHFQD+ASAXXXXXXXXXXXXXX
Sbjct: 247 TFVSAASLPVKPIVSHS----DNAQIERTTNSRENESMHFQDSASAXXXXXXXXXXXXXX 306

Query: 303 XXXXXXLANKDMNWDARDSGFDL----SPPANSTPICSHHIDHQFKAGE----------- 362
           XXXXXX                      PPANSTP  S++++HQFKAGE           
Sbjct: 307 XXXXXXXXXXXXXXXXXXXXXXXXXGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGR 366

Query: 363 ------ERTTNVNTDYEMAYRRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPP 403
                 E T NVNTDYEMAYRRHSYNPTDIKFDESDCEEET+M+D AD G SRPPD NPP
Sbjct: 367 CSSLKNEETRNVNTDYEMAYRRHSYNPTDIKFDESDCEEETEMEDEADRGVSRPPDRNPP 422

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BLAST of Carg00547 vs. TAIR10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 301.2 bits (770), Expect = 9.5e-82
Identity = 222/497 (44.67%), Postives = 266/497 (53.52%), Query Frame = 0


Query: 3   AADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQS 62
           A+ ++ +++K  +SL RRGFNSSKCKTAAKMAVARIKL+RNKR   VKQMRRDIA+LLQS
Sbjct: 7   ASAQTKKLMKFSLSLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQS 66

Query: 63  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA 122
           GQDATARIRVEHVIREQN+ AANEIIELFCEL+V+RL+II KQ++CP DLKEG+ASLIFA
Sbjct: 67  GQDATARIRVEHVIREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFA 126

Query: 123 APRCSEIPEL----------------SALTN----------LIDKLSVRTPAGEVKLKVM 182
           APRCSEIPEL                SA T+          LIDKLSVR P GE KLK+M
Sbjct: 127 APRCSEIPELGDLRDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIM 186

Query: 183 KEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDGPR 242
           KEIAKE Q++WDTTE+E+ELL P EE I                            DGPR
Sbjct: 187 KEIAKEFQVDWDTTETEQELLKPQEESI----------------------------DGPR 246

Query: 243 SFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXXXX 302
            FVSA+SLPV   AA ++       + R       + H+ DT SA               
Sbjct: 247 KFVSASSLPVN-RAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAEAATELAKQAVAAA 306

Query: 303 XXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQ--------------------- 362
                LA +    D+ +  F +S         S H  HQ                     
Sbjct: 307 QVASLLATRR---DSSNKEFSVS---------SDHSTHQKXXXXXXXXXXXXXXXXXXXX 366

Query: 363 -------FKAGEE----------RTTNVN-TDYEMAY------------RRHSYNP---- 403
                   K G E              +N +DYE  Y            RRHSYNP    
Sbjct: 367 XXXXXYYAKPGAENRGMGRRHSYNNPGINESDYEEEYTNTEAEAKETMRRRHSYNPRSVP 426

[Close]

BLAST of Carg00547 vs. TAIR10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 173.3 bits (438), Expect = 3.0e-43
Identity = 95/225 (42.22%), Postives = 134/225 (59.56%), Query Frame = 0


Query: 16  SLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHV 75
           S   +GF ++KCKT  K+ + RIKL+RN+REA +KQMRR+IA LL++GQ+ATARIRVEH+
Sbjct: 6   SFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHI 65

Query: 76  IREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPEL--- 135
           IRE+ ++AA EI+ELFCEL+  RL II  QRECP DLKE ++S+ FAAPRCS++ EL   
Sbjct: 66  IREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQV 125

Query: 136 -----------------------------------------------------SALTNLI 185
                                                                +++  L+
Sbjct: 126 QILFVSKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNSILQLV 185

[Close]

BLAST of Carg00547 vs. TAIR10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 167.5 bits (423), Expect = 1.6e-41
Identity = 87/194 (44.85%), Postives = 130/194 (67.01%), Query Frame = 0


Query: 17  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVI 76
           L  RG   +KCKT+  +A+AR+KLL+NKR+  +K M+++IA  LQ+GQ+  ARIRVEHVI
Sbjct: 7   LFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVI 66

Query: 77  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSALT 136
           RE N+ AA EI+ELFCE ++AR+ I+  ++ECP +L+E +AS+IFAAPRCSE+P+L  + 
Sbjct: 67  REMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIK 126

Query: 137 NL--------------------------IDKLSVRTPAGEVKLKVMKEIAKEHQIEWDTT 185
           NL                          I+KLS  +P+G  +LK++KEIA+E+ + WD++
Sbjct: 127 NLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSS 186

[Close]

BLAST of Carg00547 vs. TAIR10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 153.3 bits (386), Expect = 3.2e-37
Identity = 83/194 (42.78%), Postives = 123/194 (63.40%), Query Frame = 0


Query: 17  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVI 76
           +L+RGF  +KCKTA +MA +R+K+L+NK+E  +KQ+RR++A LL+SGQ  TARIRVEHV+
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 77  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSALT 136
           RE+  +AA E+I ++CEL+V RL +I  Q+ CP DLKE V S++FA+ R S++PELS + 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 137 N--------------------------LIDKLSVRTPAGEVKLKVMKEIAKEHQIEW--- 179
                                      L++KLS + P G  K+K++  IA+EH + W   
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQ 183

[Close]

BLAST of Carg00547 vs. TAIR10
Match: AT4G29440.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 146.4 bits (368), Expect = 3.9e-35
Identity = 79/197 (40.10%), Postives = 125/197 (63.45%), Query Frame = 0


Query: 17  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVI 76
           +L R F  +KCK A +MA +R+K+L+NK++  +KQ+RR++A LL+SGQ  TA+IRVEHV+
Sbjct: 4   VLHRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRVEHVV 63

Query: 77  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSALT 136
           RE+  +AA E++ ++CEL+VARL +I  Q+ CP DLKE VAS+++A+ R +++ ELS + 
Sbjct: 64  REEKTVAAYELVGIYCELLVARLGVIDSQKTCPNDLKEAVASVLYASQRLTDVGELSDIV 123

Query: 137 N--------------------------LIDKLSVRTPAGEVKLKVMKEIAKEHQIEWDTT 188
                                      L++KLSV+ P G  K+K++ EIA +H + W   
Sbjct: 124 KHFSAKYGKDFVSAAIGLQPDSGVSRLLVEKLSVKAPDGPTKIKILTEIATQHNVTW--- 183

[Close]

BLAST of Carg00547 vs. Swiss-Prot
Match: sp|P53990|IST1_HUMAN (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.8e-17
Identity = 58/178 (32.58%), Postives = 98/178 (55.06%), Query Frame = 0


Query: 17  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVI 76
           +L  GF + + +   ++ + R+KLL  K+    ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 77  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRC-SEIPELSAL 136
           RE  ++ A EI+EL+C+L++AR  +I   +E  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 137 TN-------------------------LIDKLSVRTPAGEVKLKVMKEIAKEHQIEWD 169
            +                         L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 121 ADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

[Close]

BLAST of Carg00547 vs. Swiss-Prot
Match: sp|Q9CX00|IST1_MOUSE (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.8e-17
Identity = 58/178 (32.58%), Postives = 98/178 (55.06%), Query Frame = 0


Query: 17  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVI 76
           +L  GF + + +   ++ + R+KLL  K+    ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 77  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRC-SEIPELSAL 136
           RE  ++ A EI+EL+C+L++AR  +I   +E  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 137 TN-------------------------LIDKLSVRTPAGEVKLKVMKEIAKEHQIEWD 169
            +                         L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 121 ADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

[Close]

BLAST of Carg00547 vs. Swiss-Prot
Match: sp|Q5R6G8|IST1_PONAB (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.8e-17
Identity = 58/178 (32.58%), Postives = 98/178 (55.06%), Query Frame = 0


Query: 17  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVI 76
           +L  GF + + +   ++ + R+KLL  K+    ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 77  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRC-SEIPELSAL 136
           RE  ++ A EI+EL+C+L++AR  +I   +E  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 137 TN-------------------------LIDKLSVRTPAGEVKLKVMKEIAKEHQIEWD 169
            +                         L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 121 ADQLCAKYSKGYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

[Close]

BLAST of Carg00547 vs. Swiss-Prot
Match: sp|Q568Z6|IST1_RAT (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.8e-17
Identity = 58/178 (32.58%), Postives = 98/178 (55.06%), Query Frame = 0


Query: 17  LLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVI 76
           +L  GF + + +   ++ + R+KLL  K+    ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 77  REQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRC-SEIPELSAL 136
           RE  ++ A EI+EL+C+L++AR  +I   +E  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 137 TN-------------------------LIDKLSVRTPAGEVKLKVMKEIAKEHQIEWD 169
            +                         L+ KLSV  P   +  + + EIAK + + ++
Sbjct: 121 ADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYE 178

[Close]

BLAST of Carg00547 vs. Swiss-Prot
Match: sp|Q54I39|IST1L_DICDI (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.0e-16
Identity = 57/189 (30.16%), Postives = 106/189 (56.08%), Query Frame = 0


Query: 22  FNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQSGQDATARIRVEHVIREQNV 81
           ++S K K   K+AV+RI++L+NK+   V+  +R++A LL+   + +ARIRVE +IR++ +
Sbjct: 8   YDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYL 67

Query: 82  LAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFAAPRCSEIPELSALTN---- 141
           +   +IIE+ CEL+ AR+++I    E P ++KE + +L++++ R  +IPEL  + N    
Sbjct: 68  IECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKA 127

Query: 142 ---------------------LIDKLSVRTPAGEVKLKVMKEIAKEHQIEWDTTESEKEL 186
                                ++ KLS  TP   +  + + EIA++  ++W  ++     
Sbjct: 128 KYGKGLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWCGSD----- 187

[Close]

BLAST of Carg00547 vs. TrEMBL
Match: tr|A0A0A0KKW3|A0A0A0KKW3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507450 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.1e-131
Identity = 307/448 (68.53%), Postives = 329/448 (73.44%), Query Frame = 0


Query: 3   AADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQS 62
           AA RS++I+K+FI++LR GFNSSKCKTAAKMAVARIKLLRNKREA VKQMRRDIALLLQS
Sbjct: 7   AAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQS 66

Query: 63  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA 122
           GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQR+CPADLKEGVASLIFA
Sbjct: 67  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFA 126

Query: 123 APRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLKVM 182
           APRCSEIPELSAL N                          LIDKLSVRTP GEVKLK+M
Sbjct: 127 APRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIM 186

Query: 183 KEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDGPR 242
           KEIAKEH+IEWDTTESEKELL P EELI                            +GPR
Sbjct: 187 KEIAKEHKIEWDTTESEKELLKPSEELI----------------------------EGPR 246

Query: 243 SFVSAASLPVKPAAAASQSAGNDAQIER-RNGREDESMHFQDTASAXXXXXXXXXXXXXX 302
           +FVSAASLPVKP  + S    ++AQIER  N RE+ESMHFQD+ASAXXXXXXXXXXXXXX
Sbjct: 247 TFVSAASLPVKPIVSHS----DNAQIERTTNSRENESMHFQDSASAXXXXXXXXXXXXXX 306

Query: 303 XXXXXXLANKDMNWDARDSGFDL----SPPANSTPICSHHIDHQFKAGE----------- 362
           XXXXXX                      PPANSTP  S++++HQFKAGE           
Sbjct: 307 XXXXXXXXXXXXXXXXXXXXXXXXXGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGR 366

Query: 363 ------ERTTNVNTDYEMAYRRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPP 403
                 E T NVNTDYEMAYRRHSYNPTDIKFDESDCEEET+M+D AD G SRPPD NPP
Sbjct: 367 CSSLKNEETRNVNTDYEMAYRRHSYNPTDIKFDESDCEEETEMEDEADRGVSRPPDRNPP 422

[Close]

BLAST of Carg00547 vs. TrEMBL
Match: tr|A0A1S3B2X8|A0A1S3B2X8_CUCME (IST1-like protein OS=Cucumis melo OX=3656 GN=LOC103485176 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 7.6e-126
Identity = 299/447 (66.89%), Postives = 316/447 (70.69%), Query Frame = 0


Query: 3   AADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQS 62
           AA RS++I+K+FI++LR GFNSSKCKTAAKMAVARIKLLRNKREA VKQMRRDIALLLQS
Sbjct: 7   AAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQS 66

Query: 63  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA 122
           GQDATARIRVEHVIREQNVLAANE+IELFCELVVARLSIIAKQR+CPADLKEGVASLIFA
Sbjct: 67  GQDATARIRVEHVIREQNVLAANEVIELFCELVVARLSIIAKQRQCPADLKEGVASLIFA 126

Query: 123 APRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLKVM 182
           APRCSEIPELSAL N                          LIDKLSVRTP GEVKLK+M
Sbjct: 127 APRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIM 186

Query: 183 KEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDGPR 242
           KEIAKEH+IEWDTTESEKELL PPEELI                            +GPR
Sbjct: 187 KEIAKEHKIEWDTTESEKELLKPPEELI----------------------------EGPR 246

Query: 243 SFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASA---XXXXXXXXXXXX 302
           +FVSAASLPVKP  + S    ++AQIER    E+ SMHFQDTASA   XXXXXXXXXXXX
Sbjct: 247 TFVSAASLPVKPIVSHS----DNAQIERTTNCENGSMHFQDTASAAXXXXXXXXXXXXXX 306

Query: 303 XXXXXXXXLANKDMNWDARDSGFDLSPPANSTPICSHHIDHQFKAGE------------- 362
           XXXXXXXX                               DHQFKAGE             
Sbjct: 307 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDHQFKAGEDGTAPTQSFGRCF 366

Query: 363 ----ERTTNVNTDYEMAYRRHSYNPTDIKFDESDCEEETQMDDGADGGDSRPPDGNPPPI 404
               E T NVNTDYEMAYRRHSYNPTDIKFDESDCEEET+MDD A  G SRPPD NPPP 
Sbjct: 367 SLKNEETRNVNTDYEMAYRRHSYNPTDIKFDESDCEEETEMDDEAGRGVSRPPDRNPPPA 421

[Close]

BLAST of Carg00547 vs. TrEMBL
Match: tr|A0A061DFK0|A0A061DFK0_THECC (Regulator of Vps4 activity in the MVB pathway protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_000107 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.1e-98
Identity = 256/478 (53.56%), Postives = 293/478 (61.30%), Query Frame = 0


Query: 2   SAADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQ 61
           +A   S +++K  +SL R GFNSSKCKTAAKMAVARIKLLRNKR+  VKQMRRDIALLLQ
Sbjct: 6   AATAHSKKLMKLTLSLFRNGFNSSKCKTAAKMAVARIKLLRNKRQVVVKQMRRDIALLLQ 65

Query: 62  SGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIF 121
           SGQDATARIRVEHVIREQNVLAANE IELFCELVVARLSIIAK+RECPADLKEG+ASLIF
Sbjct: 66  SGQDATARIRVEHVIREQNVLAANEFIELFCELVVARLSIIAKRRECPADLKEGIASLIF 125

Query: 122 AAPRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLKV 181
           AAPRC+EIPEL A+ +                          LIDKLSV+TP GEVKLKV
Sbjct: 126 AAPRCAEIPELVAIRDIFQKKYGKDFVSAATDLRPNCGVNRLLIDKLSVKTPTGEVKLKV 185

Query: 182 MKEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDGP 241
           MKEIAKEH IEWDTTESEKELL  PEELI                            +GP
Sbjct: 186 MKEIAKEHNIEWDTTESEKELLKLPEELI----------------------------EGP 245

Query: 242 RSFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXXX 301
           R+FVSA SLPVKPA        +  + ++   R + SMHF+DT SA       XXXXXXX
Sbjct: 246 RTFVSATSLPVKPA------TNHSLETKQLMTRGEGSMHFKDTVSAAEAAEESXXXXXXX 305

Query: 302 XXXXXXLANKDMNWDARDSGF--------------DLSPPANS--------TPICSHHID 361
           XXX   LAN+D N     SG               +LS  ANS        +P+ S H+D
Sbjct: 306 XXXAAYLANRDSNLFNEASGHVKRLNDSSIITGFGNLS--ANSAGGFLPTYSPVNSQHMD 365

Query: 362 ----------------HQFKAGEERTTNVNTDYEMAYRRHSYNP----TDIKFDESDCEE 404
                           + +   EE   N N +    +RRHS N     +DIKFDESDC+E
Sbjct: 366 PPVPGRTYESRSFERSNHYPGSEEARPN-NVEVGKTFRRHSNNSPSAHSDIKFDESDCDE 425

[Close]

BLAST of Carg00547 vs. TrEMBL
Match: tr|A0A1S3UIY2|A0A1S3UIY2_VIGRR (IST1-like protein OS=Vigna radiata var. radiata OX=3916 GN=LOC106765768 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 7.4e-97
Identity = 249/444 (56.08%), Postives = 291/444 (65.54%), Query Frame = 0


Query: 3   AADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQS 62
           A+ R+ R++K  +SLLR GFNSSKCKTAAKMAVARIKLLRNKRE  V+QMRRDIALLLQS
Sbjct: 7   ASARTKRLVKLTLSLLRLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDIALLLQS 66

Query: 63  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA 122
           GQDATARIRVEHV+REQN+LAANE IELFCEL+VARLSIIAKQRECPADLKEG+ASLIFA
Sbjct: 67  GQDATARIRVEHVMREQNILAANEFIELFCELIVARLSIIAKQRECPADLKEGIASLIFA 126

Query: 123 APRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLKVM 182
           APRCSEIPEL +L N                          LI+KLSVRTP GEVKLKV+
Sbjct: 127 APRCSEIPELVSLKNIFEKKYGKDFVSAAVDLRPSCGVNRQLIEKLSVRTPPGEVKLKVL 186

Query: 183 KEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDGPR 242
           KEIAKEHQI+WDTTESEKELL PPEE I                            DGPR
Sbjct: 187 KEIAKEHQIDWDTTESEKELLKPPEEQI----------------------------DGPR 246

Query: 243 SFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXXXX 302
           +FVSA+SLPVKP+   S  +   A      GR+ +++H++D+ SA  XXXXXXXXXXXXX
Sbjct: 247 TFVSASSLPVKPSTNVSVESNKPAATRLSGGRKMDAVHYEDSRSAAEXXXXXXXXXXXXX 306

Query: 303 XXXXXLANKDMNWDAR----DSGFDLSPPANSTPIC-SHHIDHQFKAGEERTTNVNTDYE 362
           XXXXX   K+ N   +       F  + P+    +  S  +    +   E  +  N    
Sbjct: 307 XXXXXXXKKEYNEAPQPYIYSGTFQANNPSTEDKMYRSQSLPRSDQMKSEEDSLPNQYGG 366

Query: 363 MAYRRHSYNPT----DIKFDESDCEEETQMDDGADGGDSRPPDGNPPPIPSS-------- 403
             +RRHSY+PT    DIKFDESDC+EE + +       + PP+  PPPIPSS        
Sbjct: 367 NDHRRHSYHPTSAHSDIKFDESDCDEENEAEAPR---PTLPPNRLPPPIPSSLAKQDSSI 419

[Close]

BLAST of Carg00547 vs. TrEMBL
Match: tr|A0A2N9I1V1|A0A2N9I1V1_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS45906 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 9.7e-97
Identity = 258/470 (54.89%), Postives = 288/470 (61.28%), Query Frame = 0


Query: 3   AADRSVRIIKKFISLLRRGFNSSKCKTAAKMAVARIKLLRNKREAAVKQMRRDIALLLQS 62
           A   +  I+K  +SL RRGFNSSKCKTAAKMAVARIKLLRNKRE  VKQMRRDIALLLQS
Sbjct: 292 ATSHAKNIMKIGLSLFRRGFNSSKCKTAAKMAVARIKLLRNKREVVVKQMRRDIALLLQS 351

Query: 63  GQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRECPADLKEGVASLIFA 122
           GQDATA+IRVEHVIREQNVLAANE IELFCELVVARLSII++QRECPADLKEG+AS+IFA
Sbjct: 352 GQDATAQIRVEHVIREQNVLAANEFIELFCELVVARLSIISQQRECPADLKEGIASIIFA 411

Query: 123 APRCSEIPELSALTN--------------------------LIDKLSVRTPAGEVKLKVM 182
           +PRCSEIPEL AL N                          LIDKLSVRTPAGEVKLK+M
Sbjct: 412 SPRCSEIPELVALRNIFEKKYGKDFVSAATDLRPNCGVNRMLIDKLSVRTPAGEVKLKIM 471

Query: 183 KEIAKEHQIEWDTTESEKELLNPPEELIVCSILPLFLSSDDNIVISYRCFRLSFDQDGPR 242
           KEIAKEHQI+WDTTESE ELL PPEE I                            +GPR
Sbjct: 472 KEIAKEHQIQWDTTESEMELLKPPEERI----------------------------EGPR 531

Query: 243 SFVSAASLPVKPAAAASQSAGNDAQIERRNGREDESMHFQDTASAXXXXXXXXXXXXXXX 302
           SFVSA SLP+KP    S +  N       + RE+ +MHF+DTASA XXXXXXXXXXXXXX
Sbjct: 532 SFVSATSLPMKPQPTQS-AEPNKPTTRSTSNRENGNMHFEDTASAAXXXXXXXXXXXXXX 591

Query: 303 XXXXXLANKDMNWDARDSGFD------LSPPANSTPICSHHIDHQFKA------------ 362
           XXXXX ANK+ N     S  D         P + +P+ S ++ HQ KA            
Sbjct: 592 XXXXXXANKNFNQTTEASSLDDKLNDSWVMPNHHSPVNSQNMGHQQKAHGRTYASQSFRT 651

Query: 363 -----GEERTTNVNTDYEMAYRRHSYN--------------PTDIKFDESDCEEETQMDD 403
                 EE T   N +    YRRHSYN                DIKFDESDC+EE +M++
Sbjct: 652 TPFGSSEEETPTSNVNSGKVYRRHSYNAPPXXXXXXXXXXXXXDIKFDESDCDEEIEMEE 711

The following BLAST results are available for this feature:

BLAST of Carg00547 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950275.1	1.1e-181	87.41	IST1-like protein [Cucurbita moschata]	[more]
XP_023544297.1	1.7e-177	86.01	IST1-like protein [Cucurbita pepo subsp. pepo]	[more]
XP_022977299.1	2.3e-174	84.15	IST1-like protein isoform X1 [Cucurbita maxima]	[more]
XP_022977300.1	2.3e-174	84.15	IST1-like protein isoform X2 [Cucurbita maxima]	[more]
XP_004134834.1	3.1e-131	68.53	PREDICTED: IST1-like protein [Cucumis sativus] >KGN48972.1 hypothetical protein ...	[more]

BLAST of Carg00547 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G35730.1	9.5e-82	44.67	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.1	3.0e-43	42.22	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G25420.1	1.6e-41	44.85	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT2G19710.1	3.2e-37	42.78	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT4G29440.1	3.9e-35	40.10	Regulator of Vps4 activity in the MVB pathway protein	[more]

BLAST of Carg00547 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P53990\|IST1_HUMAN	7.8e-17	32.58	IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1	[more]
sp\|Q9CX00\|IST1_MOUSE	7.8e-17	32.58	IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1	[more]
sp\|Q5R6G8\|IST1_PONAB	7.8e-17	32.58	IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1	[more]
sp\|Q568Z6\|IST1_RAT	7.8e-17	32.58	IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1	[more]
sp\|Q54I39\|IST1L_DICDI	1.0e-16	30.16	IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1	[more]

BLAST of Carg00547 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KKW3\|A0A0A0KKW3_CUCSA	2.1e-131	68.53	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507450 PE=4 SV=1	[more]
tr\|A0A1S3B2X8\|A0A1S3B2X8_CUCME	7.6e-126	66.89	IST1-like protein OS=Cucumis melo OX=3656 GN=LOC103485176 PE=4 SV=1	[more]
tr\|A0A061DFK0\|A0A061DFK0_THECC	5.1e-98	53.56	Regulator of Vps4 activity in the MVB pathway protein isoform 1 OS=Theobroma cac...	[more]
tr\|A0A1S3UIY2\|A0A1S3UIY2_VIGRR	7.4e-97	56.08	IST1-like protein OS=Vigna radiata var. radiata OX=3916 GN=LOC106765768 PE=4 SV=...	[more]
tr\|A0A2N9I1V1\|A0A2N9I1V1_FAGSY	9.7e-97	54.89	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS45906 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0015031	protein transport

Vocabulary: **INTERPRO**
Term	Definition
IPR005061	Ist1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00547-RA	Carg00547-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 259..279
None	No IPR available	GENE3D	G3DSA:1.20.1260.60		coord: 139..177 e-value: 1.5E-6 score: 29.8 coord: 15..138 e-value: 2.4E-50 score: 172.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 341..387
None	No IPR available	PANTHER	PTHR12161:SF19	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 213..292
None	No IPR available	PANTHER	PTHR12161:SF19	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 11..138
None	No IPR available	PANTHER	PTHR12161:SF19	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 314..402
None	No IPR available	PANTHER	PTHR12161:SF19	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 137..185
IPR005061	Vacuolar protein sorting-associated protein Ist1	PFAM	PF03398	Ist1	coord: 28..138 e-value: 6.6E-42 score: 143.1
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	UNCHARACTERIZED DUF292	coord: 137..185
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	UNCHARACTERIZED DUF292	coord: 314..402
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	UNCHARACTERIZED DUF292	coord: 11..138
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	UNCHARACTERIZED DUF292	coord: 213..292

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg00547	Cla014333	Watermelon (97103) v1	carwmB0407
Carg00547	Csa6G507450	Cucumber (Chinese Long) v2	carcuB0441
Carg00547	MELO3C007956	Melon (DHL92) v3.5.1	carmeB0414
Carg00547	ClCG01G020720	Watermelon (Charleston Gray)	carwcgB0397
Carg00547	CSPI06G32300	Wild cucumber (PI 183967)	carcpiB0446
Carg00547	Cucsa.044080	Cucumber (Gy14) v1	carcgyB0466
Carg00547	CmaCh07G003960	Cucurbita maxima (Rimu)	carcmaB0571
Carg00547	CmaCh03G006380	Cucurbita maxima (Rimu)	carcmaB0567
Carg00547	CmoCh07G003900	Cucurbita moschata (Rifu)	carcmoB0554
Carg00547	Lsi01G005510	Bottle gourd (USVL1VR-Ls)	carlsiB364
Carg00547	Cp4.1LG10g10350	Cucurbita pepo (Zucchini)	carcpeB0554
Carg00547	Cp4.1LG19g08550	Cucurbita pepo (Zucchini)	carcpeB0563
Carg00547	CsGy6G031850	Cucumber (Gy14) v2	carcgybB0418
Carg00547	MELO3C007956.2	Melon (DHL92) v3.6.1	carmedB0418
Carg00547	CsaV3_6G047650	Cucumber (Chinese Long) v3	carcucB0442
Carg00547	Cla97C01G020220	Watermelon (97103) v2	carwmbB0426
Carg00547	Bhi03G000586	Wax gourd	carwgoB0488

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg00547	Carg07926	Silver-seed gourd	carcarB425

The following block(s) are covering this gene:


Gene	Organism	Block
Carg00547	Watermelon (97103) v2	carwmbB0427
Carg00547	Melon (DHL92) v3.6.1	carmedB0414
Carg00547	Silver-seed gourd	carcarB189
Carg00547	Silver-seed gourd	carcarB370
Carg00547	Silver-seed gourd	carcarB422
Carg00547	Cucumber (Gy14) v2	carcgybB0413
Carg00547	Cucumber (Gy14) v1	carcgyB0472
Carg00547	Cucurbita maxima (Rimu)	carcmaB0560
Carg00547	Cucurbita maxima (Rimu)	carcmaB0564
Carg00547	Cucurbita moschata (Rifu)	carcmoB0547
Carg00547	Cucurbita moschata (Rifu)	carcmoB0549
Carg00547	Cucurbita moschata (Rifu)	carcmoB0551
Carg00547	Cucurbita pepo (Zucchini)	carcpeB0559
Carg00547	Cucurbita pepo (Zucchini)	carcpeB0561
Carg00547	Cucurbita pepo (Zucchini)	carcpeB0575
Carg00547	Wild cucumber (PI 183967)	carcpiB0441
Carg00547	Cucumber (Chinese Long) v3	carcucB0438
Carg00547	Bottle gourd (USVL1VR-Ls)	carlsiB368
Carg00547	Melon (DHL92) v3.5.1	carmeB0410
Carg00547	Watermelon (Charleston Gray)	carwcgB0395
Carg00547	Watermelon (97103) v1	carwmB0398