CSPI06G32300 (gene) Wild cucumber (PI 183967)

NameCSPI06G32300
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationChr6 : 27239419 .. 27241615 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCGTTTTCTTTTATGCCTCCCACCGTCTCCTTCTCTTCTCTCCTCCAAAGACCGTTTCACTTTTTTCACACTCCCTTTCTGCTGCTCTTCTTCTTCCTTAATCATTACCCCTTCTTTTTCCTTTGGCCATGAGCGCCGCCACTGAAGCTGCCGCTCGTTCCCTCAAGATCGTCAAGCAGTTCATCACCATCCTCCGCTGTGGCTTCAACTCCTCAAAATGGTACTCCTTTTGTTTCTTTTAAATGGGTTTCGGGTTTTTTTCAATCTGGGTAAATGGAGCCCATTGATTTTGAAATTTTCTGAGAAATGGGGTTTTGCTTTTTTCATGAAATTTTGTTTTATTTTGGGGTGGTTTTGATGACAGTAAAACGGCGGCGAAAATGGCGGTGGCGAGGATAAAACTACTGAGGAACAAGCGAGAGGCGGTGGTGAAGCAGATGAGACGAGACATTGCTCTGCTTCTTCAGTCCGGTCAAGACGCCACTGCTCGTATTCGGGTAGGTCATTCAGTTGATCCTCTGTTTCTAAAATGATTAAATCAGAGAGATGAAGTTTTGACAATTGTTTTTGTAGGTTGAACATGTGATAAGAGAACAGAATGTTTTGGCTGCTAATGAGATTATCGAGCTCTTCTGTGAGTTAGTTGTAGCTAGATTATCAATAATAGCAAAACAAAGGTACTTTTGAGAAGAATTGCCCTAATAGAAACTTTGCAATGGCTGGATTTGATTGTTGATTGTTGAGTATTGAATTTTTGCTGTAGGCAATGTCCGGCAGATCTAAAAGAAGGGGTTGCTAGTTTGATCTTTGCGGCTCCGAGGTGCTCAGAAATTCCGGAACTTTCTGCACTTAGGAATGTTTTTGAAAAGAAATATGGGAAGGATTTTGTCTCTGCTGCCGTTGATCTGAGGCCAAACTGTGGAGTGAATCGGCTGGTATAACTACATTTATAGTTCTATTTATTACATTTCACATGATTGAGTTTGTGTTGAATTGTTTGTATTTGTTTACAGCTCATTGACAAGCTCTCGGTTCGAACTCCCACGGGTGAAGTGAAGCTTAAAATAATGAAGGAGATTGCTAAGGAGCACAAGATTGAATGGGATACCACAGAATCTGAGAAAGAGCTGCTAAAGCCTTCTGAAGAACTTATTGTCTGTTATATTCTCCATATCTTCCAATCTACTCTGATGGTGAATTCTTTATGTTAATAATATATTCAATTGTGGTTTGAACAGGAAGGGCCTCGGACTTTCGTAAGTGCTGCCAGCTTACCTGTGAAACCTATAGTAAGCCATTCAGATAATGCTCAAATTGAAAGGTAAGGAAGGAATACTAACCATGGGAACCATTTGATAGATTCAATTGGAGCCAGCATTTTTGGTTAACTTCTGCCGTCACTTTTTTATGCAGAACGACAAACAGTAGAGAGAATGAAAGTATGCACTTTCAAGATTCAGCATCAGCTGCAGAAGCTGCTGCAAAAGCAGCAAAGCAAGCAATTGCTGCTGCAGAGGCAGCTGCCTATTTGGCAAACAAAGACTTGAATCGAGATGCTGGAGACTCAGGTTTCAATCTTGGATTTGGAGGAGGCCCTCCTGCCAATTCCACCCCAACCAGGTCTTATAATATGAATCATCAGTTCAAGGCAGGAGAAGATGGGACAGCCCCAACTCAGAGCTTTGGCAGGTGCTCCTCCCTTAAGAATGAGGAGACAAGGAATGTAAATACAGATTACGAGATGGCTTACAGGAGACATAGCTACAATCCTACAGACATAAAGTTTGATGAATCAGATTGTGAAGAAGAAACTGAAATGGAGGACGAAGCTGACAGAGGGGTTAGTCGACCTCCTGATCGGAATCCTCCACCAGCACCTTCATCTCGTGTTCACCCCAAACTGCCTGATTATGACACACTTGCAGCTCGTTTCGAAGCCCTCAAGTACAGGAAAGCCTGAACTTACTTAGAATCTTGCTTTGATTTTGTGGGTTTGGTAGGTGCGCATGTTAACTACTCGACCTTTTTGCATTCAAATAAACCTAAAACCAGTAGTTTAAGATTCATTTGTTGTTACACTACCCTATATAATCAAATACTGTAAACAGATAAGAGATGATGTTCAGGAAGCAGCTTTTAAGAATATTTGTTGAAATATATTGATGAATTTTTTAACCTAATGTTCAGTCAACAAC

mRNA sequence

ATGAGCGCCGCCACTGAAGCTGCCGCTCGTTCCCTCAAGATCGTCAAGCAGTTCATCACCATCCTCCGCTGTGGCTTCAACTCCTCAAAATGTAAAACGGCGGCGAAAATGGCGGTGGCGAGGATAAAACTACTGAGGAACAAGCGAGAGGCGGTGGTGAAGCAGATGAGACGAGACATTGCTCTGCTTCTTCAGTCCGGTCAAGACGCCACTGCTCGTATTCGGGTTGAACATGTGATAAGAGAACAGAATGTTTTGGCTGCTAATGAGATTATCGAGCTCTTCTGTGAGTTAGTTGTAGCTAGATTATCAATAATAGCAAAACAAAGGCAATGTCCGGCAGATCTAAAAGAAGGGGTTGCTAGTTTGATCTTTGCGGCTCCGAGGTGCTCAGAAATTCCGGAACTTTCTGCACTTAGGAATGTTTTTGAAAAGAAATATGGGAAGGATTTTGTCTCTGCTGCCGTTGATCTGAGGCCAAACTGTGGAGTGAATCGGCTGCTCATTGACAAGCTCTCGGTTCGAACTCCCACGGGTGAAGTGAAGCTTAAAATAATGAAGGAGATTGCTAAGGAGCACAAGATTGAATGGGATACCACAGAATCTGAGAAAGAGCTGCTAAAGCCTTCTGAAGAACTTATTGAAGGGCCTCGGACTTTCGTAAGTGCTGCCAGCTTACCTGTGAAACCTATAGTAAGCCATTCAGATAATGCTCAAATTGAAAGAACGACAAACAGTAGAGAGAATGAAAGTATGCACTTTCAAGATTCAGCATCAGCTGCAGAAGCTGCTGCAAAAGCAGCAAAGCAAGCAATTGCTGCTGCAGAGGCAGCTGCCTATTTGGCAAACAAAGACTTGAATCGAGATGCTGGAGACTCAGGTTTCAATCTTGGATTTGGAGGAGGCCCTCCTGCCAATTCCACCCCAACCAGGTCTTATAATATGAATCATCAGTTCAAGGCAGGAGAAGATGGGACAGCCCCAACTCAGAGCTTTGGCAGGTGCTCCTCCCTTAAGAATGAGGAGACAAGGAATGTAAATACAGATTACGAGATGGCTTACAGGAGACATAGCTACAATCCTACAGACATAAAGTTTGATGAATCAGATTGTGAAGAAGAAACTGAAATGGAGGACGAAGCTGACAGAGGGGTTAGTCGACCTCCTGATCGGAATCCTCCACCAGCACCTTCATCTCGTGTTCACCCCAAACTGCCTGATTATGACACACTTGCAGCTCGTTTCGAAGCCCTCAAGTACAGGAAAGCCTGA

Coding sequence (CDS)

ATGAGCGCCGCCACTGAAGCTGCCGCTCGTTCCCTCAAGATCGTCAAGCAGTTCATCACCATCCTCCGCTGTGGCTTCAACTCCTCAAAATGTAAAACGGCGGCGAAAATGGCGGTGGCGAGGATAAAACTACTGAGGAACAAGCGAGAGGCGGTGGTGAAGCAGATGAGACGAGACATTGCTCTGCTTCTTCAGTCCGGTCAAGACGCCACTGCTCGTATTCGGGTTGAACATGTGATAAGAGAACAGAATGTTTTGGCTGCTAATGAGATTATCGAGCTCTTCTGTGAGTTAGTTGTAGCTAGATTATCAATAATAGCAAAACAAAGGCAATGTCCGGCAGATCTAAAAGAAGGGGTTGCTAGTTTGATCTTTGCGGCTCCGAGGTGCTCAGAAATTCCGGAACTTTCTGCACTTAGGAATGTTTTTGAAAAGAAATATGGGAAGGATTTTGTCTCTGCTGCCGTTGATCTGAGGCCAAACTGTGGAGTGAATCGGCTGCTCATTGACAAGCTCTCGGTTCGAACTCCCACGGGTGAAGTGAAGCTTAAAATAATGAAGGAGATTGCTAAGGAGCACAAGATTGAATGGGATACCACAGAATCTGAGAAAGAGCTGCTAAAGCCTTCTGAAGAACTTATTGAAGGGCCTCGGACTTTCGTAAGTGCTGCCAGCTTACCTGTGAAACCTATAGTAAGCCATTCAGATAATGCTCAAATTGAAAGAACGACAAACAGTAGAGAGAATGAAAGTATGCACTTTCAAGATTCAGCATCAGCTGCAGAAGCTGCTGCAAAAGCAGCAAAGCAAGCAATTGCTGCTGCAGAGGCAGCTGCCTATTTGGCAAACAAAGACTTGAATCGAGATGCTGGAGACTCAGGTTTCAATCTTGGATTTGGAGGAGGCCCTCCTGCCAATTCCACCCCAACCAGGTCTTATAATATGAATCATCAGTTCAAGGCAGGAGAAGATGGGACAGCCCCAACTCAGAGCTTTGGCAGGTGCTCCTCCCTTAAGAATGAGGAGACAAGGAATGTAAATACAGATTACGAGATGGCTTACAGGAGACATAGCTACAATCCTACAGACATAAAGTTTGATGAATCAGATTGTGAAGAAGAAACTGAAATGGAGGACGAAGCTGACAGAGGGGTTAGTCGACCTCCTGATCGGAATCCTCCACCAGCACCTTCATCTCGTGTTCACCCCAAACTGCCTGATTATGACACACTTGCAGCTCGTTTCGAAGCCCTCAAGTACAGGAAAGCCTGA
BLAST of CSPI06G32300 vs. Swiss-Prot
Match: IST1L_DICDI (IST1-like protein OS=Dictyostelium discoideum GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 5.8e-23
Identity = 66/217 (30.41%), Postives = 127/217 (58.53%), Query Frame = 1

Query: 26  FNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQNV 85
           ++S K K   K+AV+RI++L+NK+  +V+  +R++A LL+   + +ARIRVE +IR++ +
Sbjct: 8   YDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYL 67

Query: 86  LAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFEK 145
           +   +IIE+ CEL+ AR+++I    + P ++KE + +L++++ R  +IPEL  ++N  + 
Sbjct: 68  IECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKA 127

Query: 146 KYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTTE--SE 205
           KYGK   + A +   +  VN  ++ KLS  TP   +  + + EIA++  ++W  ++    
Sbjct: 128 KYGKGLENEA-NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWCGSDYPPP 187

Query: 206 KELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI 241
            +L+ P   +++     +     P   I+ H    QI
Sbjct: 188 PQLIMPQPIIVQQQPQILQ----PPPQIIHHQQQPQI 218

BLAST of CSPI06G32300 vs. Swiss-Prot
Match: IST1_MOUSE (IST1 homolog OS=Mus musculus GN=Ist1 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 9.2e-21
Identity = 64/181 (35.36%), Postives = 108/181 (59.67%), Query Frame = 1

Query: 21  ILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L  GF + + +   ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRC-SEIPELSAL 140
           RE  ++ A EI+EL+C+L++AR  +I   ++  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 141 RNVFEKKYGKDFVSAAVDLRPN--CGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEW 199
            +    KY K++       R N    VN  L+ KLSV  P   +  + + EIAK + + +
Sbjct: 121 ADQLCAKYSKEYGKLC---RTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPY 178

BLAST of CSPI06G32300 vs. Swiss-Prot
Match: IST1_HUMAN (IST1 homolog OS=Homo sapiens GN=IST1 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 9.2e-21
Identity = 64/181 (35.36%), Postives = 108/181 (59.67%), Query Frame = 1

Query: 21  ILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L  GF + + +   ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRC-SEIPELSAL 140
           RE  ++ A EI+EL+C+L++AR  +I   ++  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 141 RNVFEKKYGKDFVSAAVDLRPN--CGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEW 199
            +    KY K++       R N    VN  L+ KLSV  P   +  + + EIAK + + +
Sbjct: 121 ADQLCAKYSKEYGKLC---RTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPY 178

BLAST of CSPI06G32300 vs. Swiss-Prot
Match: IST1_RAT (IST1 homolog OS=Rattus norvegicus GN=Ist1 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 9.2e-21
Identity = 64/181 (35.36%), Postives = 108/181 (59.67%), Query Frame = 1

Query: 21  ILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L  GF + + +   ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRC-SEIPELSAL 140
           RE  ++ A EI+EL+C+L++AR  +I   ++  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 141 RNVFEKKYGKDFVSAAVDLRPN--CGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEW 199
            +    KY K++       R N    VN  L+ KLSV  P   +  + + EIAK + + +
Sbjct: 121 ADQLCAKYSKEYGKLC---RTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPY 178

BLAST of CSPI06G32300 vs. Swiss-Prot
Match: IST1_PONAB (IST1 homolog OS=Pongo abelii GN=IST1 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.1e-20
Identity = 64/181 (35.36%), Postives = 107/181 (59.12%), Query Frame = 1

Query: 21  ILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L  GF + + +   ++ + R+KLL  K+  + ++ R++IA  L +G+D  ARIRVEH+I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRC-SEIPELSAL 140
           RE  ++ A EI+EL+C+L++AR  +I   ++  + L E V++LI+AAPR  SE+ EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 141 RNVFEKKYGKDFVSAAVDLRPN--CGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEW 199
            +    KY K +       R N    VN  L+ KLSV  P   +  + + EIAK + + +
Sbjct: 121 ADQLCAKYSKGYGKLC---RTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPY 178

BLAST of CSPI06G32300 vs. TrEMBL
Match: A0A0A0KKW3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G507450 PE=4 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 3.0e-228
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI 240
           VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI 240

Query: 241 ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLGFG 300
           ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLGFG
Sbjct: 241 ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLGFG 300

Query: 301 GGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEMAYRRHSYN 360
           GGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEMAYRRHSYN
Sbjct: 301 GGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEMAYRRHSYN 360

Query: 361 PTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY 420
           PTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY
Sbjct: 361 PTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY 420

Query: 421 RKA 424
           RKA
Sbjct: 421 RKA 423

BLAST of CSPI06G32300 vs. TrEMBL
Match: A0A061DFK0_THECC (Regulator of Vps4 activity in the MVB pathway protein isoform 1 OS=Theobroma cacao GN=TCM_000107 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 7.1e-137
Identity = 298/450 (66.22%), Postives = 338/450 (75.11%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+ A+ A A S K++K  +++ R GFNSSKCKTAAKMAVARIKLLRNKR+ VVKQMRRDI
Sbjct: 1   MTVASAATAHSKKLMKLTLSLFRNGFNSSKCKTAAKMAVARIKLLRNKRQVVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANE IELFCELVVARLSIIAK+R+CPADLKEG+
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEFIELFCELVVARLSIIAKRRECPADLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRC+EIPEL A+R++F+KKYGKDFVSAA DLRPNCGVNRLLIDKLSV+TPTGE
Sbjct: 121 ASLIFAAPRCAEIPELVAIRDIFQKKYGKDFVSAATDLRPNCGVNRLLIDKLSVKTPTGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI 240
           VKLK+MKEIAKEH IEWDTTESEKELLK  EELIEGPRTFVSA SLPVKP  +HS   + 
Sbjct: 181 VKLKVMKEIAKEHNIEWDTTESEKELLKLPEELIEGPRTFVSATSLPVKPATNHSLETKQ 240

Query: 241 ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLN---------RDAG 300
             T   R   SMHF+D+ SAAEAA ++AK+AIAAA+AAAYLAN+D N         +   
Sbjct: 241 LMT---RGEGSMHFKDTVSAAEAAEESAKKAIAAAQAAAYLANRDSNLFNEASGHVKRLN 300

Query: 301 DSGFNLGFGGGPPANST----PTRS-YNMNHQFKAGEDGTAPTQSFGRCSSLK-NEETRN 360
           DS    GFG    ANS     PT S  N  H        T  ++SF R +    +EE R 
Sbjct: 301 DSSIITGFGN-LSANSAGGFLPTYSPVNSQHMDPPVPGRTYESRSFERSNHYPGSEEARP 360

Query: 361 VNTDYEMAYRRHSYNP----TDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSS-- 420
            N +    +RRHS N     +DIKFDESDC+EE E ED A   +  PP+R+PPP PSS  
Sbjct: 361 NNVEVGKTFRRHSNNSPSAHSDIKFDESDCDEEIETED-ASGSIKCPPERSPPPVPSSYD 420

Query: 421 ------RVHPKLPDYDTLAARFEALKYRKA 424
                 RVHPKLPDYD LAARFEALKYRK+
Sbjct: 421 KQDSIHRVHPKLPDYDDLAARFEALKYRKS 445

BLAST of CSPI06G32300 vs. TrEMBL
Match: W9SMS7_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_026594 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.8e-133
Identity = 290/456 (63.60%), Postives = 340/456 (74.56%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+ AT A +++ K+VK  +++ R GFNS KCKTAAKMAVARIKLLRNKRE VVKQMRRDI
Sbjct: 1   MTVATAATSQTKKLVKLGLSLFRRGFNSPKCKTAAKMAVARIKLLRNKREVVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQD+TARIRVEHVIREQNVLAANE IELFCEL+V+RLSIIAK+R+CPADLKEG+
Sbjct: 61  ALLLQSGQDSTARIRVEHVIREQNVLAANEFIELFCELIVSRLSIIAKRRECPADLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPEL A+R+VFEKKYGKDFVSAA DLRPNCGVNR+LIDKLSVRTPTGE
Sbjct: 121 ASLIFAAPRCSEIPELVAIRDVFEKKYGKDFVSAATDLRPNCGVNRMLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHS----- 240
            KLKIMKEIAKE++++WDTTE+EKELLKP EELIEGPRTFVSA SLPVKP+ + S     
Sbjct: 181 FKLKIMKEIAKEYQVDWDTTETEKELLKPPEELIEGPRTFVSATSLPVKPVPNQSFDLNT 240

Query: 241 ----DNAQIERTTNSRENES-MHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDA 300
                ++ + RTT S ++ S M+FQDSA AA+AAAK A +AIAAA+AAA+LANKDLNR +
Sbjct: 241 NKLTTSSSLPRTTTSGDDGSHMYFQDSAGAAKAAAKCANEAIAAAKAAAFLANKDLNRTS 300

Query: 301 GDSGFNLGFGGGPPA----NSTPTRSYN-----MNHQFKAGEDGTAPTQSFGRCSSLKNE 360
                       PP+    NS    S N     +NHQ+         +Q+F    +  N 
Sbjct: 301 SQP---------PPSFDNNNSNNNPSINIGYEEINHQYNKASGRAFESQNFNGPHTPSNM 360

Query: 361 ET--RNVNTDYEMAYRRHSYNP----TDIKFDESDC-EEETEMEDEADRGVS---RPPDR 420
           ET   N N      +RRHSYN     +DIKFDESDC EE+ E+E E     S   +PP R
Sbjct: 361 ETLMNNNNKHSGRTHRRHSYNAPSAHSDIKFDESDCDEEDEEIEAETPPNGSTCFQPPQR 420

Query: 421 NPPPAPSSR-----VHPKLPDYDTLAARFEALKYRK 423
            PPP P  +     VHPKLPDYD+LAARFEALK+R+
Sbjct: 421 APPPVPLKQDSPHSVHPKLPDYDSLAARFEALKHRR 447

BLAST of CSPI06G32300 vs. TrEMBL
Match: A0A0S3R3T5_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G317600 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.4e-132
Identity = 284/438 (64.84%), Postives = 337/438 (76.94%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+ A+EA+AR+ ++VK  +++LR GFNSSKCKTAAKMAVARIKLLRNKRE VV+QMRRDI
Sbjct: 1   MTVASEASARTKRLVKLTLSLLRLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQDATARIRVEHV+REQNVLAANE IELFCEL+VARLSIIAKQR+CPADLKEG+
Sbjct: 61  ALLLQSGQDATARIRVEHVMREQNVLAANEFIELFCELIVARLSIIAKQRECPADLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPEL +LRN+FEKKYGKDFVSAAVDLRP+CGVNR LI+KLSVRTP GE
Sbjct: 121 ASLIFAAPRCSEIPELVSLRNIFEKKYGKDFVSAAVDLRPSCGVNRQLIEKLSVRTPPGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPI--VSHSDNA 240
           VKLK++KEIAKEH+I+WDTTESEKELLKP EE I+GPRTFVSA+SLPVKP   VS   N 
Sbjct: 181 VKLKVLKEIAKEHQIDWDTTESEKELLKPPEEPIDGPRTFVSASSLPVKPSTNVSVESNR 240

Query: 241 QIERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLG 300
              R +   + +++H++DS SAAEAAA+AAK+AIAAAE AAY+A K+ N    ++     
Sbjct: 241 PATRLSGGGKTDAVHYEDSRSAAEAAAEAAKKAIAAAEVAAYMAKKEYN----EAPQPYV 300

Query: 301 FGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDY-EMAYRRH 360
           + G   AN+  T            ED    +QS  R   +K+E+  ++   Y    +RRH
Sbjct: 301 YSGTFQANNPST------------EDKMYRSQSLPRSDQVKSED--SLPKQYGGNDHRRH 360

Query: 361 SYNPT----DIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSS---------RVHPK 420
           SY+PT    DIKFDESDC+EE E E+      + PP+R PPP PSS         RVHPK
Sbjct: 361 SYHPTSANSDIKFDESDCDEENEAEEPRP---TLPPNRLPPPVPSSLAKQDSSIHRVHPK 417

Query: 421 LPDYDTLAARFEALKYRK 423
           LPDYD LAARF+ALK+RK
Sbjct: 421 LPDYDELAARFDALKFRK 417

BLAST of CSPI06G32300 vs. TrEMBL
Match: A0A0L9TNY4_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g171800 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.4e-132
Identity = 284/438 (64.84%), Postives = 337/438 (76.94%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+ A+EA+AR+ ++VK  +++LR GFNSSKCKTAAKMAVARIKLLRNKRE VV+QMRRDI
Sbjct: 1   MTVASEASARTKRLVKLTLSLLRLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQDATARIRVEHV+REQNVLAANE IELFCEL+VARLSIIAKQR+CPADLKEG+
Sbjct: 61  ALLLQSGQDATARIRVEHVMREQNVLAANEFIELFCELIVARLSIIAKQRECPADLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPEL +LRN+FEKKYGKDFVSAAVDLRP+CGVNR LI+KLSVRTP GE
Sbjct: 121 ASLIFAAPRCSEIPELVSLRNIFEKKYGKDFVSAAVDLRPSCGVNRQLIEKLSVRTPPGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPI--VSHSDNA 240
           VKLK++KEIAKEH+I+WDTTESEKELLKP EE I+GPRTFVSA+SLPVKP   VS   N 
Sbjct: 181 VKLKVLKEIAKEHQIDWDTTESEKELLKPPEEPIDGPRTFVSASSLPVKPSTNVSVESNR 240

Query: 241 QIERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLG 300
              R +   + +++H++DS SAAEAAA+AAK+AIAAAE AAY+A K+ N    ++     
Sbjct: 241 PATRLSGGGKTDAVHYEDSRSAAEAAAEAAKKAIAAAEVAAYMAKKEYN----EAPQPYV 300

Query: 301 FGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDY-EMAYRRH 360
           + G   AN+  T            ED    +QS  R   +K+E+  ++   Y    +RRH
Sbjct: 301 YSGTFQANNPST------------EDKMYRSQSLPRSDQVKSED--SLPKQYGGNDHRRH 360

Query: 361 SYNPT----DIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSS---------RVHPK 420
           SY+PT    DIKFDESDC+EE E E+      + PP+R PPP PSS         RVHPK
Sbjct: 361 SYHPTSANSDIKFDESDCDEENEAEEPRP---TLPPNRLPPPVPSSLAKQDSSIHRVHPK 417

Query: 421 LPDYDTLAARFEALKYRK 423
           LPDYD LAARF+ALK+RK
Sbjct: 421 LPDYDELAARFDALKFRK 417

BLAST of CSPI06G32300 vs. TAIR10
Match: AT4G35730.1 (AT4G35730.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 385.2 bits (988), Expect = 5.3e-107
Identity = 243/467 (52.03%), Postives = 304/467 (65.10%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+AA  A+A++ K++K  +++ R GFNSSKCKTAAKMAVARIKL+RNKR  VVKQMRRDI
Sbjct: 1   MTAAEVASAQTKKLMKFSLSLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           A+LLQSGQDATARIRVEHVIREQN+ AANEIIELFCEL+V+RL+II KQ+QCP DLKEG+
Sbjct: 61  AVLLQSGQDATARIRVEHVIREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPEL  LR++F KKYGKDFVSAA DLRP+CGVNR+LIDKLSVR P GE
Sbjct: 121 ASLIFAAPRCSEIPELGDLRDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVK--PIVSHSDNA 240
            KLKIMKEIAKE +++WDTTE+E+ELLKP EE I+GPR FVSA+SLPV    I    D  
Sbjct: 181 YKLKIMKEIAKEFQVDWDTTETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPT 240

Query: 241 QIERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLG 300
           +    + S  + + H+ D+ SAAEAA + AKQA+AAA+ A+ LA +   RD+ +  F++ 
Sbjct: 241 KAVPRSTSSMSINTHYHDTESAAEAATELAKQAVAAAQVASLLATR---RDSSNKEFSVS 300

Query: 301 FGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSF-----------GRCSSLKNEETRNVN 360
                    +    ++ +H     +   + T S+           GR  S  N       
Sbjct: 301 SDHSTHQKDSQYMDHHHHHPGSRRQSRDSETSSYYAKPGAENRGMGRRHSYNNPGIN--E 360

Query: 361 TDYEMAY------------RRHSYNPTDIKFDESDCEEETEMEDEADRGVSRPPDRN--- 420
           +DYE  Y            RRHSYNP  +    +   +  E +   +      P +    
Sbjct: 361 SDYEEEYTNTEAEAKETMRRRHSYNPRSVPPPATSEIKFDESDYYEEETEPDEPSQGRVS 420

Query: 421 -------PPPAPSS----------RVHPKLPDYDTLAARFEALKYRK 423
                  PP AP S          +VHPKLPDYD LAARFEA+++ K
Sbjct: 421 SLPPNRAPPQAPQSGESRQDSSGHQVHPKLPDYDILAARFEAIRHSK 462

BLAST of CSPI06G32300 vs. TAIR10
Match: AT1G34220.1 (AT1G34220.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 216.5 bits (550), Expect = 3.2e-56
Identity = 129/308 (41.88%), Postives = 192/308 (62.34%), Query Frame = 1

Query: 25  GFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQN 84
           GF ++KCKT  K+ + RIKL+RN+REA +KQMRR+IA LL++GQ+ATARIRVEH+IRE+ 
Sbjct: 11  GFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEK 70

Query: 85  VLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFE 144
           ++AA EI+ELFCEL+  RL II  QR+CP DLKE ++S+ FAAPRCS++ EL  ++ +F 
Sbjct: 71  MMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFV 130

Query: 145 KKYGKDFVSAAVDLRPNCGVNR------------------------------LLIDKLSV 204
            KYGK+FV+AA +L+P+ GVNR                               L++ LSV
Sbjct: 131 SKYGKEFVAAASELKPDSGVNRKTESLIFIAWFSLVETRDLFMFLYFSNSILQLVELLSV 190

Query: 205 RTPTGEVKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSH 264
           R P+ E KLK++KEIA+EH+++WD   +E +L K  E+L++GP+ F   + LP+    + 
Sbjct: 191 RAPSPETKLKLLKEIAEEHELDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNE 250

Query: 265 SDN-----AQIERTTNSRENESMHFQDSASAAEAAAKAAKQAIA--AAEAAAY-LANKDL 295
             N     A  E++ +  E + + F +  +        A    A  AA++A+Y   + DL
Sbjct: 251 KTNLTSLSAAKEKSDSDSEYDILDFPEVPNVLLRPTPGATSVNAPDAAKSASYEHTSHDL 310

BLAST of CSPI06G32300 vs. TAIR10
Match: AT1G25420.1 (AT1G25420.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 115/272 (42.28%), Postives = 183/272 (67.28%), Query Frame = 1

Query: 25  GFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVIREQN 84
           G   +KCKT+  +A+AR+KLL+NKR+  +K M+++IA  LQ+GQ+  ARIRVEHVIRE N
Sbjct: 11  GIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIREMN 70

Query: 85  VLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALRNVFE 144
           + AA EI+ELFCE ++AR+ I+  +++CP +L+E +AS+IFAAPRCSE+P+L  ++N+F 
Sbjct: 71  LWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLFG 130

Query: 145 KKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTTESEK 204
            KYGK+F+  A +LRP+ GVNR +I+KLS  +P+G  +LK++KEIA+E+ + WD++ +E 
Sbjct: 131 TKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNWDSSATEA 190

Query: 205 ELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQ--IERTTNSRENESMHFQDSA--SA 264
           E +K  E+L+ G      A  +  +  +S S  +Q    +++ SRE ES+  + +     
Sbjct: 191 EFMKSHEDLLGG------AKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQK 250

Query: 265 AEAAAKAAKQAIAAAEAAAYLANKDLNRDAGD 293
            +A    +K   ++   +A+ A  D  R+  D
Sbjct: 251 LQAQNPVSKSMPSSKLTSAFQAPPDTRRNQTD 276

BLAST of CSPI06G32300 vs. TAIR10
Match: AT2G19710.1 (AT2G19710.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 194.9 bits (494), Expect = 1.0e-49
Identity = 112/270 (41.48%), Postives = 174/270 (64.44%), Query Frame = 1

Query: 21  ILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L+ GF  +KCKTA +MA +R+K+L+NK+E  +KQ+RR++A LL+SGQ  TARIRVEHV+
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALR 140
           RE+  +AA E+I ++CEL+V RL +I  Q+ CP DLKE V S++FA+ R S++PELS + 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 141 NVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTT 200
             F  KYGKDF ++AV+LRP+ GV+RLL++KLS + P G  K+KI+  IA+EH + W+  
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWE-A 183

Query: 201 ESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHS----DNAQIERTTNSR--ENESMHF 260
           +S  E      EL+ G  +F  A+S+ +   ++ +     N     T N+    +E  H 
Sbjct: 184 QSFVESDPKDTELLNGANSFQPASSMNMDSSINSNKEQPPNIHAPATVNAHHGSSERHHS 243

Query: 261 QDSASAAEAAAKAAKQAIAAAEAAAYLANK 285
            +++ A    + +    + + +A  Y  +K
Sbjct: 244 PENSYANGGRSSSRSNNVTSGKADDYYHSK 272

BLAST of CSPI06G32300 vs. TAIR10
Match: AT4G29440.1 (AT4G29440.1 Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 192.6 bits (488), Expect = 5.0e-49
Identity = 103/226 (45.58%), Postives = 153/226 (67.70%), Query Frame = 1

Query: 21  ILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDIALLLQSGQDATARIRVEHVI 80
           +L   F  +KCK A +MA +R+K+L+NK++  +KQ+RR++A LL+SGQ  TA+IRVEHV+
Sbjct: 4   VLHRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRVEHVV 63

Query: 81  REQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGVASLIFAAPRCSEIPELSALR 140
           RE+  +AA E++ ++CEL+VARL +I  Q+ CP DLKE VAS+++A+ R +++ ELS + 
Sbjct: 64  REEKTVAAYELVGIYCELLVARLGVIDSQKTCPNDLKEAVASVLYASQRLTDVGELSDIV 123

Query: 141 NVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGEVKLKIMKEIAKEHKIEWDTT 200
             F  KYGKDFVSAA+ L+P+ GV+RLL++KLSV+ P G  K+KI+ EIA +H + W+  
Sbjct: 124 KHFSAKYGKDFVSAAIGLQPDSGVSRLLVEKLSVKAPDGPTKIKILTEIATQHNVTWE-- 183

Query: 201 ESEKELLKPSEELIEG-PRTFVSAA------SLPVKPIVSHSDNAQ 240
                    +E L+E  P+  +SA+      S P   I S S   Q
Sbjct: 184 ---------AESLVESDPKETMSASGASSSVSQPATGIKSESSRIQ 218

BLAST of CSPI06G32300 vs. NCBI nr
Match: gi|449434100|ref|XP_004134834.1| (PREDICTED: IST1-like protein [Cucumis sativus])

HSP 1 Score: 798.9 bits (2062), Expect = 4.3e-228
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI 240
           VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI 240

Query: 241 ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLGFG 300
           ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLGFG
Sbjct: 241 ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLGFG 300

Query: 301 GGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEMAYRRHSYN 360
           GGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEMAYRRHSYN
Sbjct: 301 GGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEMAYRRHSYN 360

Query: 361 PTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY 420
           PTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY
Sbjct: 361 PTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY 420

Query: 421 RKA 424
           RKA
Sbjct: 421 RKA 423

BLAST of CSPI06G32300 vs. NCBI nr
Match: gi|659080620|ref|XP_008440890.1| (PREDICTED: IST1-like protein [Cucumis melo])

HSP 1 Score: 753.1 bits (1943), Expect = 2.7e-214
Identity = 405/422 (95.97%), Postives = 410/422 (97.16%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI
Sbjct: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANE+IELFCELVVARLSIIAKQRQCPADLKEGV
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEVIELFCELVVARLSIIAKQRQCPADLKEGV 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE
Sbjct: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI 240
           VKLKIMKEIAKEHKIEWDTTESEKELLKP EELIEGPRTFVSAASLPVKPIVSHSDNAQI
Sbjct: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPPEELIEGPRTFVSAASLPVKPIVSHSDNAQI 240

Query: 241 ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNLGFG 300
           ERTTN  EN SMHFQD+ASAAEAAAKAAKQAIAAAEAAAYLANKD N+DA DSGFNLGFG
Sbjct: 241 ERTTNC-ENGSMHFQDTASAAEAAAKAAKQAIAAAEAAAYLANKDRNQDARDSGFNLGFG 300

Query: 301 GGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEMAYRRHSYN 360
           G  PANST  RSYNM+HQFKAGEDGTAPTQSFGRC SLKNEETRNVNTDYEMAYRRHSYN
Sbjct: 301 GS-PANSTSNRSYNMDHQFKAGEDGTAPTQSFGRCFSLKNEETRNVNTDYEMAYRRHSYN 360

Query: 361 PTDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY 420
           PTDIKFDESDCEEETEM+DEA RGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY
Sbjct: 361 PTDIKFDESDCEEETEMDDEAGRGVSRPPDRNPPPAPSSRVHPKLPDYDTLAARFEALKY 420

Query: 421 RK 423
           RK
Sbjct: 421 RK 420

BLAST of CSPI06G32300 vs. NCBI nr
Match: gi|590702166|ref|XP_007046560.1| (Regulator of Vps4 activity in the MVB pathway protein isoform 1 [Theobroma cacao])

HSP 1 Score: 495.4 bits (1274), Expect = 1.0e-136
Identity = 298/450 (66.22%), Postives = 338/450 (75.11%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+ A+ A A S K++K  +++ R GFNSSKCKTAAKMAVARIKLLRNKR+ VVKQMRRDI
Sbjct: 1   MTVASAATAHSKKLMKLTLSLFRNGFNSSKCKTAAKMAVARIKLLRNKRQVVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQDATARIRVEHVIREQNVLAANE IELFCELVVARLSIIAK+R+CPADLKEG+
Sbjct: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEFIELFCELVVARLSIIAKRRECPADLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRC+EIPEL A+R++F+KKYGKDFVSAA DLRPNCGVNRLLIDKLSV+TPTGE
Sbjct: 121 ASLIFAAPRCAEIPELVAIRDIFQKKYGKDFVSAATDLRPNCGVNRLLIDKLSVKTPTGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHSDNAQI 240
           VKLK+MKEIAKEH IEWDTTESEKELLK  EELIEGPRTFVSA SLPVKP  +HS   + 
Sbjct: 181 VKLKVMKEIAKEHNIEWDTTESEKELLKLPEELIEGPRTFVSATSLPVKPATNHSLETKQ 240

Query: 241 ERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLN---------RDAG 300
             T   R   SMHF+D+ SAAEAA ++AK+AIAAA+AAAYLAN+D N         +   
Sbjct: 241 LMT---RGEGSMHFKDTVSAAEAAEESAKKAIAAAQAAAYLANRDSNLFNEASGHVKRLN 300

Query: 301 DSGFNLGFGGGPPANST----PTRS-YNMNHQFKAGEDGTAPTQSFGRCSSLK-NEETRN 360
           DS    GFG    ANS     PT S  N  H        T  ++SF R +    +EE R 
Sbjct: 301 DSSIITGFGN-LSANSAGGFLPTYSPVNSQHMDPPVPGRTYESRSFERSNHYPGSEEARP 360

Query: 361 VNTDYEMAYRRHSYNP----TDIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSS-- 420
            N +    +RRHS N     +DIKFDESDC+EE E ED A   +  PP+R+PPP PSS  
Sbjct: 361 NNVEVGKTFRRHSNNSPSAHSDIKFDESDCDEEIETED-ASGSIKCPPERSPPPVPSSYD 420

Query: 421 ------RVHPKLPDYDTLAARFEALKYRKA 424
                 RVHPKLPDYD LAARFEALKYRK+
Sbjct: 421 KQDSIHRVHPKLPDYDDLAARFEALKYRKS 445

BLAST of CSPI06G32300 vs. NCBI nr
Match: gi|703163224|ref|XP_010113265.1| (hypothetical protein L484_026594 [Morus notabilis])

HSP 1 Score: 483.4 bits (1243), Expect = 4.0e-133
Identity = 290/456 (63.60%), Postives = 340/456 (74.56%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+ AT A +++ K+VK  +++ R GFNS KCKTAAKMAVARIKLLRNKRE VVKQMRRDI
Sbjct: 1   MTVATAATSQTKKLVKLGLSLFRRGFNSPKCKTAAKMAVARIKLLRNKREVVVKQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQD+TARIRVEHVIREQNVLAANE IELFCEL+V+RLSIIAK+R+CPADLKEG+
Sbjct: 61  ALLLQSGQDSTARIRVEHVIREQNVLAANEFIELFCELIVSRLSIIAKRRECPADLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPEL A+R+VFEKKYGKDFVSAA DLRPNCGVNR+LIDKLSVRTPTGE
Sbjct: 121 ASLIFAAPRCSEIPELVAIRDVFEKKYGKDFVSAATDLRPNCGVNRMLIDKLSVRTPTGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKPIVSHS----- 240
            KLKIMKEIAKE++++WDTTE+EKELLKP EELIEGPRTFVSA SLPVKP+ + S     
Sbjct: 181 FKLKIMKEIAKEYQVDWDTTETEKELLKPPEELIEGPRTFVSATSLPVKPVPNQSFDLNT 240

Query: 241 ----DNAQIERTTNSRENES-MHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDA 300
                ++ + RTT S ++ S M+FQDSA AA+AAAK A +AIAAA+AAA+LANKDLNR +
Sbjct: 241 NKLTTSSSLPRTTTSGDDGSHMYFQDSAGAAKAAAKCANEAIAAAKAAAFLANKDLNRTS 300

Query: 301 GDSGFNLGFGGGPPA----NSTPTRSYN-----MNHQFKAGEDGTAPTQSFGRCSSLKNE 360
                       PP+    NS    S N     +NHQ+         +Q+F    +  N 
Sbjct: 301 SQP---------PPSFDNNNSNNNPSINIGYEEINHQYNKASGRAFESQNFNGPHTPSNM 360

Query: 361 ET--RNVNTDYEMAYRRHSYNP----TDIKFDESDC-EEETEMEDEADRGVS---RPPDR 420
           ET   N N      +RRHSYN     +DIKFDESDC EE+ E+E E     S   +PP R
Sbjct: 361 ETLMNNNNKHSGRTHRRHSYNAPSAHSDIKFDESDCDEEDEEIEAETPPNGSTCFQPPQR 420

Query: 421 NPPPAPSSR-----VHPKLPDYDTLAARFEALKYRK 423
            PPP P  +     VHPKLPDYD+LAARFEALK+R+
Sbjct: 421 APPPVPLKQDSPHSVHPKLPDYDSLAARFEALKHRR 447

BLAST of CSPI06G32300 vs. NCBI nr
Match: gi|950997337|ref|XP_014505983.1| (PREDICTED: IST1-like protein [Vigna radiata var. radiata])

HSP 1 Score: 483.0 bits (1242), Expect = 5.3e-133
Identity = 284/438 (64.84%), Postives = 337/438 (76.94%), Query Frame = 1

Query: 1   MSAATEAAARSLKIVKQFITILRCGFNSSKCKTAAKMAVARIKLLRNKREAVVKQMRRDI 60
           M+ A+EA+AR+ ++VK  +++LR GFNSSKCKTAAKMAVARIKLLRNKRE VV+QMRRDI
Sbjct: 1   MTVASEASARTKRLVKLTLSLLRLGFNSSKCKTAAKMAVARIKLLRNKREVVVRQMRRDI 60

Query: 61  ALLLQSGQDATARIRVEHVIREQNVLAANEIIELFCELVVARLSIIAKQRQCPADLKEGV 120
           ALLLQSGQDATARIRVEHV+REQN+LAANE IELFCEL+VARLSIIAKQR+CPADLKEG+
Sbjct: 61  ALLLQSGQDATARIRVEHVMREQNILAANEFIELFCELIVARLSIIAKQRECPADLKEGI 120

Query: 121 ASLIFAAPRCSEIPELSALRNVFEKKYGKDFVSAAVDLRPNCGVNRLLIDKLSVRTPTGE 180
           ASLIFAAPRCSEIPEL +L+N+FEKKYGKDFVSAAVDLRP+CGVNR LI+KLSVRTP GE
Sbjct: 121 ASLIFAAPRCSEIPELVSLKNIFEKKYGKDFVSAAVDLRPSCGVNRQLIEKLSVRTPPGE 180

Query: 181 VKLKIMKEIAKEHKIEWDTTESEKELLKPSEELIEGPRTFVSAASLPVKP---IVSHSDN 240
           VKLK++KEIAKEH+I+WDTTESEKELLKP EE I+GPRTFVSA+SLPVKP   +   S+ 
Sbjct: 181 VKLKVLKEIAKEHQIDWDTTESEKELLKPPEEQIDGPRTFVSASSLPVKPSTNVSVESNK 240

Query: 241 AQIERTTNSRENESMHFQDSASAAEAAAKAAKQAIAAAEAAAYLANKDLNRDAGDSGFNL 300
               R +  R+ +++H++DS SAAEAAA+AAK+AIAAAE AAY+A K+ N    ++    
Sbjct: 241 PAATRLSGGRKMDAVHYEDSRSAAEAAAEAAKKAIAAAEVAAYMAKKEYN----EAPQPY 300

Query: 301 GFGGGPPANSTPTRSYNMNHQFKAGEDGTAPTQSFGRCSSLKNEETRNVNTDYEMAYRRH 360
            + G   AN+  T            ED    +QS  R   +K+EE    N      +RRH
Sbjct: 301 IYSGTFQANNPST------------EDKMYRSQSLPRSDQMKSEEDSLPNQYGGNDHRRH 360

Query: 361 SYNPT----DIKFDESDCEEETEMEDEADRGVSRPPDRNPPPAPSS---------RVHPK 420
           SY+PT    DIKFDESDC+EE E   EA R  + PP+R PPP PSS         RVHPK
Sbjct: 361 SYHPTSAHSDIKFDESDCDEENEA--EAPR-PTLPPNRLPPPIPSSLAKQDSSIHRVHPK 419

Query: 421 LPDYDTLAARFEALKYRK 423
           LPDYD LAARF+ALK+RK
Sbjct: 421 LPDYDELAARFDALKFRK 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
IST1L_DICDI5.8e-2330.41IST1-like protein OS=Dictyostelium discoideum GN=DDB_G0289029 PE=3 SV=1[more]
IST1_MOUSE9.2e-2135.36IST1 homolog OS=Mus musculus GN=Ist1 PE=1 SV=1[more]
IST1_HUMAN9.2e-2135.36IST1 homolog OS=Homo sapiens GN=IST1 PE=1 SV=1[more]
IST1_RAT9.2e-2135.36IST1 homolog OS=Rattus norvegicus GN=Ist1 PE=2 SV=1[more]
IST1_PONAB2.1e-2035.36IST1 homolog OS=Pongo abelii GN=IST1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KKW3_CUCSA3.0e-228100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G507450 PE=4 SV=1[more]
A0A061DFK0_THECC7.1e-13766.22Regulator of Vps4 activity in the MVB pathway protein isoform 1 OS=Theobroma cac... [more]
W9SMS7_9ROSA2.8e-13363.60Uncharacterized protein OS=Morus notabilis GN=L484_026594 PE=4 SV=1[more]
A0A0S3R3T5_PHAAN2.4e-13264.84Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.01G317600 PE=... [more]
A0A0L9TNY4_PHAAN2.4e-13264.84Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan01g171800 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35730.15.3e-10752.03 Regulator of Vps4 activity in the MVB pathway protein[more]
AT1G34220.13.2e-5641.88 Regulator of Vps4 activity in the MVB pathway protein[more]
AT1G25420.11.1e-5142.28 Regulator of Vps4 activity in the MVB pathway protein[more]
AT2G19710.11.0e-4941.48 Regulator of Vps4 activity in the MVB pathway protein[more]
AT4G29440.15.0e-4945.58 Regulator of Vps4 activity in the MVB pathway protein[more]
Match NameE-valueIdentityDescription
gi|449434100|ref|XP_004134834.1|4.3e-228100.00PREDICTED: IST1-like protein [Cucumis sativus][more]
gi|659080620|ref|XP_008440890.1|2.7e-21495.97PREDICTED: IST1-like protein [Cucumis melo][more]
gi|590702166|ref|XP_007046560.1|1.0e-13666.22Regulator of Vps4 activity in the MVB pathway protein isoform 1 [Theobroma cacao... [more]
gi|703163224|ref|XP_010113265.1|4.0e-13363.60hypothetical protein L484_026594 [Morus notabilis][more]
gi|950997337|ref|XP_014505983.1|5.3e-13364.84PREDICTED: IST1-like protein [Vigna radiata var. radiata][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005061Ist1
Vocabulary: Biological Process
TermDefinition
GO:0015031protein transport
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport
cellular_component GO:0005575 cellular_component
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G32300.1CSPI06G32300.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161UNCHARACTERIZED DUF292coord: 15..422
score: 2.9E
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 32..196
score: 8.6
NoneNo IPR availableunknownCoilCoilcoord: 258..278
scor
NoneNo IPR availablePANTHERPTHR12161:SF19REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 15..422
score: 2.9E

The following gene(s) are paralogous to this gene:

None