CSPI07G23450 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI07G23450
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	DNA-directed RNA polymerase subunit beta
Location	Chr7 : 19709226 .. 19711777 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCCGTAATCCATATTCTCGACTAGCAAAAGGTTTAGGGAAGGTTTTAGCTCGCACCGAAACCAGTTTCGGAATCAATTACAATCTTAGAACCATGGTTGTGAAGCTCCCAGAAGACGCTTCGAAGTAAAGTCATCCACATTTTCTCATTATCTTCGTCTCTCTGTTTTCCGTCTCTCTGTTTTCTTTTGGGTGTATAATTGTTAAATTGCAGTTTTCCACCATTGTTAATTACTGACCAGTTATTAGCCAACTTGAAAAAAACTGCAGGAATGAGAAGCTAGGTGACAATTCTAATCCAATCGATTCTGACATTGATAAGCAATTCATAGGCCTTCCTATCAAGTCAGCCGTCGATAAGTTTCAACTTCTTCCTGAATTTTTGAAGGTGTAAATCATCACTCTGTTTTTGTTTGTTACGATTATCAGTTTAATATGCAGAAGACACTATAACAGTATATATTACTATGCGTTTGTTTAAATTTTAGCCTATAATTGGTTATTAATACTCATAGTGTTGACCCATCTGGTTGTAGGTAAGAGGTCTGGTCAAGCAACACTTGGATTCGTTTAACTACTTCGTGAATACAGAGATAAAAAAGATAGTCAAGGCCAACGATCGCATTGAATCCAGTACTGATCCGAGTATTTATCTTAGGTACACGTTTTTTCATTTTATATAACCGTCCTTTTGTTAAATACATGGGAGAATATTTTATATCCGAGTATTCTTTTTTTTTTTTTTGGAAAATATATTTTTCATTTTATGTAACCGTCCTCTCGTGGAAAATCTGCTGTTTTCTCTCAAACCACAATTCAGCCAGAGGAGCTGTAGACAAATTTAACCATATTAGTCTAGGCTCTTTGAATTGACAGGCTTTTATCTTTCCTGGCCGTTAGAAGATACCTCTTGATTCAACTCCTATTCAGTTGTCAATCGAGCTTTTATTGAATGGTTCTGTAAAGGGATTTGCCCTCTAGTTACTGTGTTTTTTTGTATGTTTGTTTAGTTTCGTCTTGTTTCTTTTGGCTTTAATGAGCTGCATCCTTGAGTTATTTAGCTTGCGATTATCTTGTTCCAACATGATGAGGGTGCTAAGGGGTGTCAATCTAGTTGGTGCGTCTCCTGATCCATAGGCTTCATAGATTATGCTCTTTGTATAATTCTCTTGTTCCATAAGCATTGGTCTCATTTCATATTATTTAAAAAGAGGTTGGTTTCCTTTTCAAAAAAAGGAAAAAATTTTGGTATTAGAGTTTGGATTCTTTTTTCTAGACATTTGGTATGATGAGTGTTTATTATAGTCAACGGGGACGTAATGAAGGGTTTCCTTTTCAATTCTATGATTGAGGAATTTCTTCCGCCTCCTCCTTCCTCCCCGACAACCAGTCCCCTTTGGCCACCTCCAAATATTTCAGAATGGAGCTTATGTGGCTGATTGCTGGTGTAATGCTACTCATTCTTGGAATTTGGGCCTTAGAAGAAATATGCTCGACAATGAGATTGTCAATGCGGCCTCAGCTTTAGAAATTCTTCACTCTTGGGCCCCCACTGAAAGGAATGATAGTCTTAAATGGATTCCTAACATGAATGGCAATTTCACTACAAAATCTACTTTTCTTAACTTAACTAAGAGATCCCCCAACATTGCCGTCCCCTTGATTCGTCAGATTTGGAAGAATAAAATCCCGAAGAAGGTGAAGTTTTTCTTATGGTCGCTTGCTTACAGAAGCCTCAACACCCATGAGAAACTACAAAAAAAAATCCAGAACACATTGCTTAGCCCCTCGATGTGTTGCCTATGCGCTAAAGATGAGGAAACTTTGGATCACTTATTCCTACATTGTCCCTTCACAAGAAAAGCTTGGAACACGCTGTTTGGTATTTTCGACTTGGAGTTTTGCCTTCCTAGCAAGATTGATAGATGGATGATTGAAGGTCTTAATATTAGAGGTTACAGCCCTAAAGGAAACATCTTATGGAAATGTGCGTCGCGTTCCCTTTTGTGGAGCATTTGGAAAGAAAGGAATAGCAGAATTTTTGAAGATAGATTTAATTCTTTTGATTCTTTTTGGACTGTGGTTCAACACACAGCCTCTTGGTGGAGTACGAAGTACACCAAACACTTTTGTAATTATAGCCTTTCTATGATTTTCAACAATTTGAAGGCCATTATGTCCTAGCTCTTTAGCTTCTTCCGGGGAGGGCCCTCTTGTCCCTCACCCTTAGGATGTTCTGTTTTGTTATATGAATATACTTGTCTCTTATAAAAAAAAAGAATGGAGGTTTTTAGCTGCAAAATCAACCAATCTCACTATTGTATTTGGAAGGAAAATGAAAGCTGTATCATTGAAGATGTGGAAGCCAACAAAACACCATCACTTACCAATGATCAACTCCGATGGACTCAAAATGCCATTGTTGAACTCCTTAATTCTCCAGTTGAACGCTTCTTCCAAATGCTAGCTGGCATTGTGACGGAATCCAATTGGTATGAATTTACTTCTTATCTACTGAATATAGAACGGGATCGTTCTACGATTACAATATGA

mRNA sequence

ATGGTTGTGAAGCTCCCAGAAGACGCTTCGAAGAATGAGAAGCTAGGTGACAATTCTAATCCAATCGATTCTGACATTGATAAGCAATTCATAGGCCTTCCTATCAAGTCAGCCGTCGATAAGTTTCAACTTCTTCCTGAATTTTTGAAGGTAAGAGGTCTGGTCAAGCAACACTTGGATTCGTTTAACTACTTCGTGAATACAGAGATAAAAAAGATAGTCAAGGCCAACGATCGCATTGAATCCAGTACTGATCCGAGTATTTATCTTAGGAATTTCTTCCGCCTCCTCCTTCCTCCCCGACAACCAGTCCCCTTTGGCCACCTCCAAATATTTCAGAATGGAGCTTATGTGGCTGATTGCTGGTGTAATGCTACTCATTCTTGGAATTTGGGCCTTAGAAGAAATATGCTCGACAATGAGATTGTCAATGCGGCCTCAGCTTTAGAAATTCTTCACTCTTGGGCCCCCACTGAAAGGAATGATAGTCTTAAATGGATTCCTAACATGAATGGCAATTTCACTACAAAATCTACTTTTCTTAACTTAACTAAGAGATCCCCCAACATTGCCGTCCCCTTGATTCGTCAGATTTGGAAGAATAAAATCCCGAAGAAGGTGAAGTTTTTCTTATGGTCGCTTGCTTACAGAAGCCTCAACACCCATGAGAAACTACAAAAAAAAATCCAGAACACATTGCTTAGCCCCTCGATGTGTTGCCTATGCGCTAAAGATGAGGAAACTTTGGATCACTTATTCCTACATTGTCCCTTCACAAGAAAAGCTTGGAACACGCTGTTTGGTATTTTCGACTTGGAGTTTTGCCTTCCTAGCAAGATTGATAGATGGATGATTGAAGGTCTTAATATTAGAGGTTACAGCCCTAAAGGAAACATCTTATGGAAATGTGCGTCGCGTTCCCTTTTGTGGAGCATTTGGAAAGAAAGGAATAGCAGAATTTTTGAAGATAGATTTAATTCTTTTGATTCTTTTTGGACTGTGGTTCAACACACAGCCTCTTGGTGGAGTACGAAGCCATTATGTCCTAGCTCTTTAGCTTCTTCCGGGGAGGGCCCTCTTGAAAATGAAAGCTGTATCATTGAAGATGTGGAAGCCAACAAAACACCATCACTTACCAATGATCAACTCCGATGGACTCAAAATGCCATTGTTGAACTCCTTAATTCTCCAGTTGAACGCTTCTTCCAAATGCTAGCTGGCATTGTGACGGAATCCAATTGGTATGAATTTACTTCTTATCTACTGAATATAGAACGGGATCGTTCTACGATTACAATATGA

Coding sequence (CDS)

ATGGTTGTGAAGCTCCCAGAAGACGCTTCGAAGAATGAGAAGCTAGGTGACAATTCTAATCCAATCGATTCTGACATTGATAAGCAATTCATAGGCCTTCCTATCAAGTCAGCCGTCGATAAGTTTCAACTTCTTCCTGAATTTTTGAAGGTAAGAGGTCTGGTCAAGCAACACTTGGATTCGTTTAACTACTTCGTGAATACAGAGATAAAAAAGATAGTCAAGGCCAACGATCGCATTGAATCCAGTACTGATCCGAGTATTTATCTTAGGAATTTCTTCCGCCTCCTCCTTCCTCCCCGACAACCAGTCCCCTTTGGCCACCTCCAAATATTTCAGAATGGAGCTTATGTGGCTGATTGCTGGTGTAATGCTACTCATTCTTGGAATTTGGGCCTTAGAAGAAATATGCTCGACAATGAGATTGTCAATGCGGCCTCAGCTTTAGAAATTCTTCACTCTTGGGCCCCCACTGAAAGGAATGATAGTCTTAAATGGATTCCTAACATGAATGGCAATTTCACTACAAAATCTACTTTTCTTAACTTAACTAAGAGATCCCCCAACATTGCCGTCCCCTTGATTCGTCAGATTTGGAAGAATAAAATCCCGAAGAAGGTGAAGTTTTTCTTATGGTCGCTTGCTTACAGAAGCCTCAACACCCATGAGAAACTACAAAAAAAAATCCAGAACACATTGCTTAGCCCCTCGATGTGTTGCCTATGCGCTAAAGATGAGGAAACTTTGGATCACTTATTCCTACATTGTCCCTTCACAAGAAAAGCTTGGAACACGCTGTTTGGTATTTTCGACTTGGAGTTTTGCCTTCCTAGCAAGATTGATAGATGGATGATTGAAGGTCTTAATATTAGAGGTTACAGCCCTAAAGGAAACATCTTATGGAAATGTGCGTCGCGTTCCCTTTTGTGGAGCATTTGGAAAGAAAGGAATAGCAGAATTTTTGAAGATAGATTTAATTCTTTTGATTCTTTTTGGACTGTGGTTCAACACACAGCCTCTTGGTGGAGTACGAAGCCATTATGTCCTAGCTCTTTAGCTTCTTCCGGGGAGGGCCCTCTTGAAAATGAAAGCTGTATCATTGAAGATGTGGAAGCCAACAAAACACCATCACTTACCAATGATCAACTCCGATGGACTCAAAATGCCATTGTTGAACTCCTTAATTCTCCAGTTGAACGCTTCTTCCAAATGCTAGCTGGCATTGTGACGGAATCCAATTGGTATGAATTTACTTCTTATCTACTGAATATAGAACGGGATCGTTCTACGATTACAATATGA

Homology

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BLAST of CSPI07G23450 vs. Swiss-Prot
Match: NRPC2_ARATH (DNA-directed RNA polymerase III subunit 2 OS=Arabidopsis thaliana GN=NRPC2 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.3e-21
Identity = 51/69 (73.91%), Postives = 56/69 (81.16%), Query Frame = 1


Query: 23 DSDIDKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLDSFNYFVNTEIKKIVKANDRIES 82
          D  IDK+ +  PIKS  DKFQL+PEFLKVRGLVKQHLDSFNYF+N  I KIVKAN RI S
Sbjct: 14 DHFIDKEKLSAPIKSTADKFQLVPEFLKVRGLVKQHLDSFNYFINVGIHKIVKANSRITS 73

Query: 83 STDPSIYLR 92
          + DPSIYLR
Sbjct: 74 TVDPSIYLR 82

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BLAST of CSPI07G23450 vs. Swiss-Prot
Match: RPC2_HUMAN (DNA-directed RNA polymerase III subunit RPC2 OS=Homo sapiens GN=POLR3B PE=1 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 5.9e-15
Identity = 36/61 (59.02%), Postives = 49/61 (80.33%), Query Frame = 1


Query: 31 IGLPIKSAVDKFQLLPEFLKVRGLVKQHLDSFNYFVNTEIKKIVKANDRIESSTDPSIYL 90
          +  PI +  +K++LLP FLKV+GLVKQH+DSFNYF+N EIKKI+KAN+++ S  DP  YL
Sbjct: 16 LAAPIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYL 75

Query: 91 R 92
          +
Sbjct: 76 K 76

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BLAST of CSPI07G23450 vs. Swiss-Prot
Match: RPC2_MOUSE (DNA-directed RNA polymerase III subunit RPC2 OS=Mus musculus GN=Polr3b PE=1 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 7.8e-15
Identity = 36/58 (62.07%), Postives = 48/58 (82.76%), Query Frame = 1


Query: 34 PIKSAVDKFQLLPEFLKVRGLVKQHLDSFNYFVNTEIKKIVKANDRIESSTDPSIYLR 92
          PI +  +K++LLP FLKV+GLVKQH+DSFNYF+N EIKKI+KAN+++ S  DP  YL+
Sbjct: 19 PIPTVEEKWRLLPAFLKVKGLVKQHIDSFNYFINVEIKKIMKANEKVTSDADPMWYLK 76

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BLAST of CSPI07G23450 vs. Swiss-Prot
Match: RPC2_YEAST (DNA-directed RNA polymerase III subunit RPC2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RET1 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 1.7e-14
Identity = 38/57 (66.67%), Postives = 46/57 (80.70%), Query Frame = 1


Query: 35 IKSAVDKFQLLPEFLKVRGLVKQHLDSFNYFVNTEIKKIVKANDRIESSTDPSIYLR 92
          I +A DK+ LLP FLKV+GLVKQHLDSFNYFV+T++KKI+KAN  I S  DP  YL+
Sbjct: 38 INTAQDKWHLLPAFLKVKGLVKQHLDSFNYFVDTDLKKIIKANQLILSDVDPEFYLK 94

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BLAST of CSPI07G23450 vs. Swiss-Prot
Match: RPC2_DROME (DNA-directed RNA polymerase III subunit RPC2 OS=Drosophila melanogaster GN=RpIII128 PE=2 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 5.0e-14
Identity = 35/65 (53.85%), Postives = 50/65 (76.92%), Query Frame = 1


Query: 27 DKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLDSFNYFVNTEIKKIVKANDRIESSTDP 86
          D +   +PIK   +K++L+P FL+V+GLVKQH+DSFN+F+N +IKKIVKAN+ + S  DP
Sbjct: 20 DSKDWSVPIKPLTEKWKLVPAFLQVKGLVKQHIDSFNHFINVDIKKIVKANELVTSGADP 79

Query: 87 SIYLR 92
            YL+
Sbjct: 80 LFYLK 84

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BLAST of CSPI07G23450 vs. TrEMBL
Match: A5BPI6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_008538 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-36
Identity = 78/216 (36.11%), Postives = 125/216 (57.87%), Query Frame = 1


Query: 128  SWNLGLRRNMLDNEIVNAASALEIL-HSWAPTERNDSLKWIPNMNGNFTTKSTFLNLTKR 187
            SWN   RRN+ D+EI    S ++ L H        D   W  + +G FT KS FL L++ 
Sbjct: 1091 SWNFNFRRNLSDSEIEKVESLMQSLDHIHLSPSVPDKRSWSLSSSGLFTVKSFFLALSQI 1150

Query: 188  SPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSLNTHEKLQKKIQNTLLSPSMCCLCAKDE 247
            S   +V   + +W +++P K+KFF+W +A++ +NT++ LQ +     LSP +C LC +  
Sbjct: 1151 SGLPSVFPTKLVWNSQVPFKIKFFVWLVAHKKVNTNDMLQLRRPYKALSPDICMLCMERG 1210

Query: 248  ETLDHLFLHCPFTRKAWNTLFGIFDLEFCLPSKIDRWMIEGLNIRGYSPKGNILWKCASR 307
            ET+DHLFLHC  T   W+ LF +  +++  P  +   +    N  G S +G +LW+ A  
Sbjct: 1211 ETVDHLFLHCSMTMGLWHRLFQLTKIDWVPPRSVFDMISINFNGFGSSKRGIVLWQAACI 1270

Query: 308  SLLWSIWKERNSRIFEDRFNSFDSFWTVVQHTASWW 343
            ++LW +W+ERN+RIFED+  + ++ W ++   AS W
Sbjct: 1271 AILWVVWRERNARIFEDKSRNSENLWDMIHFLASLW 1306

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BLAST of CSPI07G23450 vs. TrEMBL
Match: A5BTG1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030741 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.3e-36
Identity = 82/219 (37.44%), Postives = 126/219 (57.53%), Query Frame = 1


Query: 128  SWNLGLRRNMLDNEIVNAAS---ALEILHSWAPTERNDSLKWIPNMNGNFTTKSTFLNLT 187
            SWN   RRN+ D+EI +  S   +L+ LH        D   W  + +G FT KS FL L+
Sbjct: 1067 SWNFNFRRNLTDSEIEDLESLMRSLDRLHISPSVP--DKRSWSISPSGLFTVKSFFLALS 1126

Query: 188  KRSPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSLNTHEKLQKKIQNTLLSPSMCCLCAK 247
            + S +  V   + +W +++P KVK F+W +A++ LNT++ LQ +  +  LSP++C LC K
Sbjct: 1127 QHSESPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNTNDLLQLRRPHKALSPNICKLCMK 1186

Query: 248  DEETLDHLFLHCPFTRKAWNTLFGIFDLEFCLPSKIDRWMIEGLNIRGYSPKGNILWKCA 307
              ET+DHLFLHC  T+  W+ LF +  +++  P  I        N  G S +G +LW+ A
Sbjct: 1187 HGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSISDMFFTNFNGFGSSKRGVVLWQDA 1246

Query: 308  SRSLLWSIWKERNSRIFEDRFNSFDSFWTVVQHTASWWS 344
              +L+W +W+ERN+RIFED+  +    W  +   AS W+
Sbjct: 1247 CIALMWVVWRERNARIFEDKARNSGCLWDSICFLASLWA 1283

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BLAST of CSPI07G23450 vs. TrEMBL
Match: A5BKH7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026806 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.3e-36
Identity = 82/219 (37.44%), Postives = 126/219 (57.53%), Query Frame = 1


Query: 128 SWNLGLRRNMLDNEIVNAAS---ALEILHSWAPTERNDSLKWIPNMNGNFTTKSTFLNLT 187
           SWN   RRN+ D+EI +  S   +L+ LH        D   W  + +G FT KS FL L+
Sbjct: 445 SWNFNFRRNLTDSEIEDLESLMRSLDRLHISPSVP--DKRSWSISPSGLFTVKSFFLALS 504

Query: 188 KRSPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSLNTHEKLQKKIQNTLLSPSMCCLCAK 247
           + S +  V   + +W +++P KVK F+W +A++ LNT++ LQ +  +  LSP++C LC K
Sbjct: 505 QYSESPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNTNDLLQLRRPHKALSPNICKLCMK 564

Query: 248 DEETLDHLFLHCPFTRKAWNTLFGIFDLEFCLPSKIDRWMIEGLNIRGYSPKGNILWKCA 307
             ET+DHLFLHC  T+  W+ LF +  +++  P  I        N  G S +G +LW+ A
Sbjct: 565 HGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSISDMFFTNFNGFGSSKRGVVLWQDA 624

Query: 308 SRSLLWSIWKERNSRIFEDRFNSFDSFWTVVQHTASWWS 344
             +L W +W+ERN+RIFED+  +    W  ++  AS W+
Sbjct: 625 CIALKWVVWRERNARIFEDKARNSGFLWDSIRFLASLWA 661

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BLAST of CSPI07G23450 vs. TrEMBL
Match: A5C377_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016641 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.3e-36
Identity = 82/219 (37.44%), Postives = 128/219 (58.45%), Query Frame = 1


Query: 128  SWNLGLRRNMLDNEIVNAAS---ALEILHSWAPTERNDSLKWIPNMNGNFTTKSTFLNLT 187
            SWN   RRN+ D+EI +  S   +L+ LH  +P+   D   W  + +G FT KS FL L+
Sbjct: 1627 SWNFNFRRNLTDSEIEDLESLMRSLDRLHI-SPSVX-DKRSWSISPSGLFTVKSFFLALS 1686

Query: 188  KRSPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSLNTHEKLQKKIQNTLLSPSMCCLCAK 247
            +      V   + +W +++P KVK F+W +A++ LNT++ LQ +  +  LSP++C LC K
Sbjct: 1687 QXXEXPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNTNDLLQLRRPHKALSPNICKLCMK 1746

Query: 248  DEETLDHLFLHCPFTRKAWNTLFGIFDLEFCLPSKIDRWMIEGLNIRGYSPKGNILWKCA 307
              ET+DHLFLHC  T+  W+ LF +  +++  P  I        N  G S +G +LW+ A
Sbjct: 1747 HGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSISDMFFTNFNGFGSSKRGVVLWQDA 1806

Query: 308  SRSLLWSIWKERNSRIFEDRFNSFDSFWTVVQHTASWWS 344
              +L+W +W+ERN+RIFED+  +    W  ++  AS W+
Sbjct: 1807 CIALMWVVWRERNARIFEDKARNSGCLWDSIRFLASLWA 1843

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BLAST of CSPI07G23450 vs. TrEMBL
Match: A5BQD9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000232 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.2e-35
Identity = 77/216 (35.65%), Postives = 124/216 (57.41%), Query Frame = 1


Query: 128  SWNLGLRRNMLDNEIVNAASALEIL-HSWAPTERNDSLKWIPNMNGNFTTKSTFLNLTKR 187
            SWN   RRN+ D+EI    S ++ L H        D   W  + +G FT KS FL L++ 
Sbjct: 1691 SWNFNFRRNLSDSEIEELESLMQSLDHLHLSPXVPDKRSWSLSSSGLFTVKSFFLALSQI 1750

Query: 188  SPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSLNTHEKLQKKIQNTLLSPSMCCLCAKDE 247
            S   +V   + +W +++P K+K F+W +A++ +NT++ LQ +     LSP +C LC +  
Sbjct: 1751 SGLPSVFPTKLVWNSQVPFKIKSFVWLVAHKKVNTNDMLQLRRPYKALSPDICMLCMEQG 1810

Query: 248  ETLDHLFLHCPFTRKAWNTLFGIFDLEFCLPSKIDRWMIEGLNIRGYSPKGNILWKCASR 307
            ET+DHLFLHC  T   W+ LF +  +++  P  +   +    N  G S +G +LW+ A  
Sbjct: 1811 ETVDHLFLHCSMTMGLWHRLFQLTKIDWVPPRSVFDMISINFNGFGSSKRGIVLWQAACI 1870

Query: 308  SLLWSIWKERNSRIFEDRFNSFDSFWTVVQHTASWW 343
            ++LW +W+ERN+RIFED+  + ++ W ++   AS W
Sbjct: 1871 AILWVVWRERNARIFEDKSRNSENLWDMIHFLASLW 1906

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BLAST of CSPI07G23450 vs. TAIR10
Match: AT5G45140.1 (AT5G45140.1 nuclear RNA polymerase C2)

HSP 1 Score: 104.4 bits (259), Expect = 1.8e-22
Identity = 51/69 (73.91%), Postives = 56/69 (81.16%), Query Frame = 1


Query: 23 DSDIDKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLDSFNYFVNTEIKKIVKANDRIES 82
          D  IDK+ +  PIKS  DKFQL+PEFLKVRGLVKQHLDSFNYF+N  I KIVKAN RI S
Sbjct: 14 DHFIDKEKLSAPIKSTADKFQLVPEFLKVRGLVKQHLDSFNYFINVGIHKIVKANSRITS 73

Query: 83 STDPSIYLR 92
          + DPSIYLR
Sbjct: 74 TVDPSIYLR 82

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BLAST of CSPI07G23450 vs. TAIR10
Match: AT3G09510.1 (AT3G09510.1 Ribonuclease H-like superfamily protein)

HSP 1 Score: 63.2 bits (152), Expect = 4.7e-10
Identity = 45/175 (25.71%), Postives = 76/175 (43.43%), Query Frame = 1


Query: 156 APTERNDSLKWIPNMNGNFTTKSTFLNLTK---------RSPNIAVPLIRQIWKNKIPKK 215
           A +++ D + W  N  G +T +S +  LT            P+ ++ L  +IW   I  K
Sbjct: 111 AKSKKPDKIIWNYNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPK 170

Query: 216 VKFFLWSLAYRSLNTHEKLQKKIQNTLLSPSMCCLCAKDEETLDHLFLHCPFTRKAWNTL 275
           +K FLW    ++L T E+L  +     + PS C  C ++ E+++H    CPF   AW   
Sbjct: 171 LKHFLWRALSQALATTERLTTR--GMRIDPS-CPRCHRENESINHALFTCPFATMAWRLS 230

Query: 276 FGIFDLEFCLPSKIDRWMIEGLNIRGYSPKGNILWKCASRSLLWSIWKERNSRIF 322
                    + +  +  +   LN    +   +   K     L+W IWK RN+ +F
Sbjct: 231 DSSLIRNQLMSNDFEENISNILNFVQDTTMSD-FHKLLPVWLIWRIWKARNNVVF 281

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BLAST of CSPI07G23450 vs. TAIR10
Match: AT5G18880.1 (AT5G18880.1 RNA-directed DNA polymerase (reverse transcriptase)-related family protein)

HSP 1 Score: 62.4 bits (150), Expect = 8.0e-10
Identity = 48/181 (26.52%), Postives = 81/181 (44.75%), Query Frame = 1


Query: 160 RNDSLKWIPNMNGNFTTKSTFLNLTKRSPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSL 219
           RN +  ++P+    F+++ T+  +   SP   VP  + +W  +   +     W      L
Sbjct: 99  RNAAGSYLPS----FSSRDTWEQIRVHSPT--VPWAKVVWFKEYIPRFSLITWMSFLERL 158

Query: 220 NTHEKLQKKIQNTLLSPSMCCLCAKDEETLDHLFLHCPFTRKAWNTLFGIF--DLEFCLP 279
            T ++L+    N    PS   LC+  +ET  HLF  C F+   W      F     F LP
Sbjct: 159 PTRDRLRGWGMNI---PSSWVLCSNGDETHAHLFFECSFSLAIWEFFASKFRPSPPFGLP 218

Query: 280 SKIDRWMIEGLNIRGYSPKGNILWKCASRSLLWSIWKERNSRIFEDRFNSFDSFWTVVQH 339
           +    W+++ L +R +S     + K   +S ++ +WKERN+RIF    +S  S    +  
Sbjct: 219 AA-SSWILQ-LPLRSHS---TTILKLLLQSAVYHVWKERNARIFTSISSSASSLRLAIDR 265

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BLAST of CSPI07G23450 vs. NCBI nr
Match: gi|778690261|ref|XP_011653085.1| (PREDICTED: uncharacterized protein LOC105435174 [Cucumis sativus])

HSP 1 Score: 186.8 bits (473), Expect = 8.0e-44
Identity = 87/137 (63.50%), Postives = 106/137 (77.37%), Query Frame = 1


Query: 162 DSLKWIPNMNGNFTTKSTFLNLTKRSPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSLNT 221
           DSLKW  N +G FTTKSTFLNLTKRSP IA  L+  IWK K+PKKVKFFLWSL+YRSLNT
Sbjct: 82  DSLKWELNASGIFTTKSTFLNLTKRSP-IAFVLVCTIWKIKVPKKVKFFLWSLSYRSLNT 141

Query: 222 HEKLQKKIQNTLLSPSMCCLCAKDEETLDHLFLHCPFTRKAWNTLFGIFDLEFCLPSKID 281
           H+KLQ+K+    L+PSMCCLC ++EETLD LFLHC F  K W+ LF I +L+ CLPSK  
Sbjct: 142 HDKLQRKLHYATLNPSMCCLCFRNEETLDQLFLHCEFVMKGWSFLFRILNLDCCLPSKTA 201

Query: 282 RWMIEGLNIRGYSPKGN 299
            WM++G+N + + PK +
Sbjct: 202 SWMLDGVNSKAFRPKAS 217

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BLAST of CSPI07G23450 vs. NCBI nr
Match: gi|778730035|ref|XP_004141655.2| (PREDICTED: DNA-directed RNA polymerase III subunit RPC2 [Cucumis sativus])

HSP 1 Score: 181.8 bits (460), Expect = 2.6e-42
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1


Query: 1  MVVKLPEDASKNEKLGDNSNPIDSDIDKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLD 60
          MVVKLPEDASKNEKLGDNSNPIDSDIDKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLD
Sbjct: 1  MVVKLPEDASKNEKLGDNSNPIDSDIDKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLD 60

Query: 61 SFNYFVNTEIKKIVKANDRIESSTDPSIYLR 92
          SFNYFVNTEIKKIVKANDRIESSTDPSIYLR
Sbjct: 61 SFNYFVNTEIKKIVKANDRIESSTDPSIYLR 91

[Close]

BLAST of CSPI07G23450 vs. NCBI nr
Match: gi|659124980|ref|XP_008462447.1| (PREDICTED: DNA-directed RNA polymerase III subunit RPC2 [Cucumis melo])

HSP 1 Score: 177.6 bits (449), Expect = 4.8e-41
Identity = 89/91 (97.80%), Postives = 90/91 (98.90%), Query Frame = 1


Query: 1  MVVKLPEDASKNEKLGDNSNPIDSDIDKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLD 60
          MVVKLPEDASKNEK GD+SNPIDSDIDKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLD
Sbjct: 1  MVVKLPEDASKNEKQGDDSNPIDSDIDKQFIGLPIKSAVDKFQLLPEFLKVRGLVKQHLD 60

Query: 61 SFNYFVNTEIKKIVKANDRIESSTDPSIYLR 92
          SFNYFVNTEIKKIVKANDRIESSTDPSIYLR
Sbjct: 61 SFNYFVNTEIKKIVKANDRIESSTDPSIYLR 91

[Close]

BLAST of CSPI07G23450 vs. NCBI nr
Match: gi|147798966|emb|CAN68165.1| (hypothetical protein VITISV_008538 [Vitis vinifera])

HSP 1 Score: 162.2 bits (409), Expect = 2.1e-36
Identity = 78/216 (36.11%), Postives = 125/216 (57.87%), Query Frame = 1


Query: 128  SWNLGLRRNMLDNEIVNAASALEIL-HSWAPTERNDSLKWIPNMNGNFTTKSTFLNLTKR 187
            SWN   RRN+ D+EI    S ++ L H        D   W  + +G FT KS FL L++ 
Sbjct: 1091 SWNFNFRRNLSDSEIEKVESLMQSLDHIHLSPSVPDKRSWSLSSSGLFTVKSFFLALSQI 1150

Query: 188  SPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSLNTHEKLQKKIQNTLLSPSMCCLCAKDE 247
            S   +V   + +W +++P K+KFF+W +A++ +NT++ LQ +     LSP +C LC +  
Sbjct: 1151 SGLPSVFPTKLVWNSQVPFKIKFFVWLVAHKKVNTNDMLQLRRPYKALSPDICMLCMERG 1210

Query: 248  ETLDHLFLHCPFTRKAWNTLFGIFDLEFCLPSKIDRWMIEGLNIRGYSPKGNILWKCASR 307
            ET+DHLFLHC  T   W+ LF +  +++  P  +   +    N  G S +G +LW+ A  
Sbjct: 1211 ETVDHLFLHCSMTMGLWHRLFQLTKIDWVPPRSVFDMISINFNGFGSSKRGIVLWQAACI 1270

Query: 308  SLLWSIWKERNSRIFEDRFNSFDSFWTVVQHTASWW 343
            ++LW +W+ERN+RIFED+  + ++ W ++   AS W
Sbjct: 1271 AILWVVWRERNARIFEDKSRNSENLWDMIHFLASLW 1306

[Close]

BLAST of CSPI07G23450 vs. NCBI nr
Match: gi|147815645|emb|CAN61757.1| (hypothetical protein VITISV_030741 [Vitis vinifera])

HSP 1 Score: 161.0 bits (406), Expect = 4.7e-36
Identity = 82/219 (37.44%), Postives = 126/219 (57.53%), Query Frame = 1


Query: 128  SWNLGLRRNMLDNEIVNAAS---ALEILHSWAPTERNDSLKWIPNMNGNFTTKSTFLNLT 187
            SWN   RRN+ D+EI +  S   +L+ LH        D   W  + +G FT KS FL L+
Sbjct: 1067 SWNFNFRRNLTDSEIEDLESLMRSLDRLHISPSVP--DKRSWSISPSGLFTVKSFFLALS 1126

Query: 188  KRSPNIAVPLIRQIWKNKIPKKVKFFLWSLAYRSLNTHEKLQKKIQNTLLSPSMCCLCAK 247
            + S +  V   + +W +++P KVK F+W +A++ LNT++ LQ +  +  LSP++C LC K
Sbjct: 1127 QHSESPPVFPTKFVWNSQVPFKVKSFVWLVAHKKLNTNDLLQLRRPHKALSPNICKLCMK 1186

Query: 248  DEETLDHLFLHCPFTRKAWNTLFGIFDLEFCLPSKIDRWMIEGLNIRGYSPKGNILWKCA 307
              ET+DHLFLHC  T+  W+ LF +  +++  P  I        N  G S +G +LW+ A
Sbjct: 1187 HGETVDHLFLHCSLTKGLWHRLFQLAKMDWVSPRSISDMFFTNFNGFGSSKRGVVLWQDA 1246

Query: 308  SRSLLWSIWKERNSRIFEDRFNSFDSFWTVVQHTASWWS 344
              +L+W +W+ERN+RIFED+  +    W  +   AS W+
Sbjct: 1247 CIALMWVVWRERNARIFEDKARNSGCLWDSICFLASLWA 1283

The following BLAST results are available for this feature:

BLAST of CSPI07G23450 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NRPC2_ARATH	3.3e-21	73.91	DNA-directed RNA polymerase III subunit 2 OS=Arabidopsis thaliana GN=NRPC2 PE=2 ...	[more]
RPC2_HUMAN	5.9e-15	59.02	DNA-directed RNA polymerase III subunit RPC2 OS=Homo sapiens GN=POLR3B PE=1 SV=2	[more]
RPC2_MOUSE	7.8e-15	62.07	DNA-directed RNA polymerase III subunit RPC2 OS=Mus musculus GN=Polr3b PE=1 SV=2	[more]
RPC2_YEAST	1.7e-14	66.67	DNA-directed RNA polymerase III subunit RPC2 OS=Saccharomyces cerevisiae (strain...	[more]
RPC2_DROME	5.0e-14	53.85	DNA-directed RNA polymerase III subunit RPC2 OS=Drosophila melanogaster GN=RpIII...	[more]

BLAST of CSPI07G23450 vs. TrEMBL


Match Name	E-value	Identity	Description
A5BPI6_VITVI	1.5e-36	36.11	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_008538 PE=4 SV=1	[more]
A5BTG1_VITVI	3.3e-36	37.44	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030741 PE=4 SV=1	[more]
A5BKH7_VITVI	7.3e-36	37.44	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026806 PE=4 SV=1	[more]
A5C377_VITVI	7.3e-36	37.44	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016641 PE=4 SV=1	[more]
A5BQD9_VITVI	1.2e-35	35.65	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000232 PE=4 SV=1	[more]

BLAST of CSPI07G23450 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G45140.1	1.8e-22	73.91	nuclear RNA polymerase C2	[more]
AT3G09510.1	4.7e-10	25.71	Ribonuclease H-like superfamily protein	[more]
AT5G18880.1	8.0e-10	26.52	RNA-directed DNA polymerase (reverse transcriptase)-related family p...	[more]

BLAST of CSPI07G23450 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778690261\|ref\|XP_011653085.1\|	8.0e-44	63.50	PREDICTED: uncharacterized protein LOC105435174 [Cucumis sativus]	[more]
gi\|778730035\|ref\|XP_004141655.2\|	2.6e-42	100.00	PREDICTED: DNA-directed RNA polymerase III subunit RPC2 [Cucumis sativus]	[more]
gi\|659124980\|ref\|XP_008462447.1\|	4.8e-41	97.80	PREDICTED: DNA-directed RNA polymerase III subunit RPC2 [Cucumis melo]	[more]
gi\|147798966\|emb\|CAN68165.1\|	2.1e-36	36.11	hypothetical protein VITISV_008538 [Vitis vinifera]	[more]
gi\|147815645\|emb\|CAN61757.1\|	4.7e-36	37.44	hypothetical protein VITISV_030741 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR015712	DNA-dir_RNA_pol_su2
IPR026960	RVT-Znf

Vocabulary: **Biological Process**
Term	Definition
GO:0006351	transcription, DNA-templated

Vocabulary: **Molecular Function**
Term	Definition
GO:0003899	DNA-directed RNA polymerase activity
GO:0032549	ribonucleoside binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0032549	ribonucleoside binding
molecular_function	GO:0003677	DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G23450.1	CSPI07G23450.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015712	DNA-directed RNA polymerase, subunit 2	PANTHER	PTHR20856	DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2	coord: 11..91 score: 4.0
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 174..263 score: 4.8
None	No IPR available	GENE3D	G3DSA:3.90.1100.10		coord: 46..86 score: 1.
None	No IPR available	PANTHER	PTHR20856:SF8	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC2	coord: 11..91 score: 4.0
None	No IPR available	unknown	SSF64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 35..129 score: 4.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI07G23450	MELO3C024452	Melon (DHL92) v3.5.1	cpimeB525
CSPI07G23450	MELO3C024455.2	Melon (DHL92) v3.6.1	cpimedB512
CSPI07G23450	CsaV3_7G035430	Cucumber (Chinese Long) v3	cpicucB414
CSPI07G23450	Cucsa.361180	Cucumber (Gy14) v1	cgycpiB535
CSPI07G23450	CsGy7G021700	Cucumber (Gy14) v2	cgybcpiB331

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI07G23450	Cucurbita pepo (Zucchini)	cpecpiB158
CSPI07G23450	Bottle gourd (USVL1VR-Ls)	cpilsiB517
CSPI07G23450	Silver-seed gourd	carcpiB1063
CSPI07G23450	Watermelon (97103) v2	cpiwmbB596
CSPI07G23450	Wax gourd	cpiwgoB721
CSPI07G23450	Cucurbita maxima (Rimu)	cmacpiB387
CSPI07G23450	Cucurbita moschata (Rifu)	cmocpiB376
CSPI07G23450	Cucumber (Chinese Long) v2	cpicuB354
CSPI07G23450	Watermelon (Charleston Gray)	cpiwcgB553
CSPI07G23450	Watermelon (97103) v1	cpiwmB569