CSPI07G18350 (gene) Wild cucumber (PI 183967)

NameCSPI07G18350
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionNifU-like protein 1
LocationChr7 : 16407665 .. 16410712 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATATTTTAAGGAAAAAAATCAGCAAAACTAAAAATATTCTTCAATTGCAATGTCTCTAAAATCGTCAAATACCAATAAATAGCAATTTTACACGAAAAGCCCTCCTTGGAGTGTGCTGCAGAACAACTACAACCTCATCATCGCCTAGATTCCTCTTCGTTTCTTCTTCATTAATGGCGTCCTTAACTACCTCCGGCCTCCTCAAAACCCCTCTTAAGAATTCCCAATCTCCTGCAAATTCTCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTGCCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCAGGGCTGTACTCCGCTCAGAAATTTGAATTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGTGGGTAGATTTCTTGTCTCGTTGAATTGAATGGGTTTGTTTTAATTTCCCTTGTTAATTTAATTTTTTGTGTTGAAATTTTTGTTACTTATAAATTGGTTGTTGTTGGGGTATGTTCTTTGGTGTTTCTATAGTTTAGCAAATTGTGTTGAATATCTTTTACACATTATGGTATTTAGTAGTATCTGCACTTTAGAGATTTGAGTCTAATTCATTTCTTTCATCGAAGCAATCCTGGGATCGTATGGTTTAGGACAAAGTTGATATCTTTGTGATTCTCGGTCTGAGTATGCCCCTTCAGCCCATCTCTTTTTACATAGCCAAATAGTTGGTGTATGCTTTCTCGACGCTGGCCCGATAGAAGTGATTCCTTTTTCTCAAAAAGAATGGTCTCTTTCTTAGCATTACTGTAACCCATTTGGTCTTAGTCGTCTTGTGTGGAAGGAGCGAGATGACAGAGATTTTGCTCTTCTCACCCCTGTCCGACCACCCGATTGGTGAAGACTCGGAACAAACCACCCCGCTTTGGGAGACAACGAAGACTTTACTCGATGTCTTGATCCATCCGTAGTCTGCTAATCTGATCCCAACGTCTTTAATTCTGACGAGCTTGTCTCGGAGAGATCTCTATCTTTTGTTTGTTACACAACACTGAAGGCTCCACTAGAAAAGGAAGGAAAAAGGAAAATACCTCCAATGAGAAATCTCAAGATCAAGATTAGTAGTGCTGCAGTTCTATTTACAATCTTAAACTTTCCTAACTACAATCTTCCTCCATTAGCACTGAATCTATTGGAAATTTTGAACAGTTTAGACTTGGGCAGATGGATATGATTTTCCTTTCCTTATATGAGGGTCTTTCCAATGAAAAGGATTTACCAGAGAATAGATGATTTGATATCTATCTTTAAAGAAAACCACGAAAAGGATCCTTAATTTCACTTGTTTGTGAACACTTTTAACCAATCTTGTGGGAGATTTGGTATATATCTAACAGGAATGTTTCTCAGGACATGGGTGGGGTTCTCATGACATTTGAAGGGACTGCAGACTGATGTGGCCTTTCGGTTTCTTTCCTGCATATACTAGTTGGAGTACATTTGTTTTGTTGTCTTGGTCTAGGTAAGTTTTGGACAGCACCATCATCTTGTACATGTTACTGGTGTGTTTTATCTGTTTCTTATCAAGTAAGGTGAAAAAGGTCTCCTTGAACTAAATGGCAAAAGGAAGTCTTGTAACCCTCACTTTCATCGAAAAGGGTGTGAGTAAGAACTTTGTTGATTTGATGTTTAACTGCTTTGTATTCCCATGAAGTCATGAACAAAAAGTAATAATGATAAACATTTTTAACTAAAAATGTTTGAATCGATCTTCAGGAGCATGCGGTAGCTGCCCTAGCTCAACAACGACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGTGAGTTTTCTGCAGACGAGCAAATACAAAATTGGCTGATGTCAAAATTCAAACAAAAACAAAGTGAGCTGATTTAGTTAGGCAATTTAGTATTAATCCTGGATGTTGCACAAATGCAGGCAGTAAACAGTCACCTTGACATATTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCAATTAATGGTGGCGATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAACCTTTCCTCAGGTTTCTGAAGCAAAGAGAATATGTATAGCTGCTTCATTTATTGAAAAGTTGGTGCTCATCTTTTTCATAGGCATGTAATGATCTCTTTTCCAATAAATGTTATATATTATTGATAGCCATATCTTATGCTCATAGAAAGGAAGTGCACTTATTTTAAAAGGGTAAACATTGTTCACACAAAATTTTGTTCTGATATAGCAGATTTACATTAACCTTATATCCTTGGACCAAGATTATTTTCTCTGTAAATTACCAGAGCCTCAACTGGCAACATACTCCTGCTAATATATGTAGATTGTTCATTTTAAAAAAAAAATTTACATCATCATTCTTTTAAATCTACTGAAAATGGTACGATCCAACGAATACTGGTATACAAAAATGGAAGTAATGAAAGAAAAAAAAAAGTTTCTCCTCATTTCTGGGTCCATTTACATATAATACACCATCATATAAGAGAACTATCCTGCTGACTCTGCGAGCAGAAAGATAAAACTAGCTAATAGATGTAAGCAAACCAGAGCTTTGGCTTCGTGATGAAGATAAAATGAAGGAAACAGACCGCTGTTAACTCGATTCCACAGATGCTAATGACTCCACAGATGAGGAAATCACGCGTCTTAACCACACCAGCATCAAAGTTCAGTTCAGATAACGATGCTCCAAAAGCTAAATTATATTCTTCAGAGTCTTCCAGCAAAAGC

mRNA sequence

ATGGCGTCCTTAACTACCTCCGGCCTCCTCAAAACCCCTCTTAAGAATTCCCAATCTCCTGCAAATTCTCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTGCCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCAGGGCTGTACTCCGCTCAGAAATTTGAATTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCTAGCTCAACAACGACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGCAGTAAACAGTCACCTTGACATATTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCAATTAATGGTGGCGATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

Coding sequence (CDS)

ATGGCGTCCTTAACTACCTCCGGCCTCCTCAAAACCCCTCTTAAGAATTCCCAATCTCCTGCAAATTCTCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTGCCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCAGGGCTGTACTCCGCTCAGAAATTTGAATTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGATGGAGTAGTTTCCCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCTAGCTCAACAACGACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGCAGTAAACAGTCACCTTGACATATTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCAATTAATGGTGGCGATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA
BLAST of CSPI07G18350 vs. Swiss-Prot
Match: NIFU1_ARATH (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 3.2e-65
Identity = 142/230 (61.74%), Postives = 169/230 (73.48%), Query Frame = 1

Query: 1   MASLTTS--GLLKTPLKNSQS----PANSLQYPPFA-YGQHQLPVSLKRAFPGRAIRASP 60
           MASL TS  G  +  +K+S +    P  S Q P F  +   +  +S    F   AI  S 
Sbjct: 2   MASLATSISGSFRILVKSSSTRNGFPVISDQNPSFVLFANKRRHISRTAIFHRSAISGSS 61

Query: 61  SNSGPSTTSS---PGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLK 120
                S  +S    GLYSAQ F+LT  NVDLVLEDVRP+LI+DGGNVDVVSVEDGVVSLK
Sbjct: 62  QGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLK 121

Query: 121 LVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPA 180
           L GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV+DEE K+ T EAVN+HLDILRPA
Sbjct: 122 LQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEVKQITVEAVNAHLDILRPA 181

Query: 181 IRNYGGSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           I+NYGGSVEV+S+ G DC+VKY GPESIG G++AAIKE+F DI+NV F+S
Sbjct: 182 IKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKDISNVTFTS 231

BLAST of CSPI07G18350 vs. Swiss-Prot
Match: NIFU2_ARATH (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 9.1e-20
Identity = 57/148 (38.51%), Postives = 84/148 (56.76%), Query Frame = 1

Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRP-AIRNYGGSVEVISINGGDCLVKY 193
            EK  + V       +E   E   E +   L+ +RP  I    GS++++ I      ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGVKAAIKERFPDITNV 217
            GP     ++   V   ++E+ P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

BLAST of CSPI07G18350 vs. Swiss-Prot
Match: NIFU1_ORYSJ (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-19
Identity = 70/179 (39.11%), Postives = 98/179 (54.75%), Query Frame = 1

Query: 45  PGRAIRASPSNSGPSTT--SSPGL--YSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVV 104
           P   + A+P++  P     +  GL   +A +  LT GNV+ VL+ VRPYL ADGG+V + 
Sbjct: 42  PATRLVAAPNHQRPPLVVGAIAGLDPVTAVQLPLTAGNVESVLDQVRPYLTADGGDVALH 101

Query: 105 SVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEP-KETTPEA 164
            +   VV LKL GACGSCPSS  T+K GIER L EK  D V  +  V D+E   E   E 
Sbjct: 102 EIAGNVVRLKLQGACGSCPSSLITIKRGIERRLMEKIPD-VAAVEPVTDKETGLELNEEN 161

Query: 165 VNSHLDILRPAIRNY-GGSVEVISINGGDCLVKYEGPESIGTGVKAAI----KERFPDI 214
           V   L+ +RP +    GG ++ + I G    V+  GP ++   V+ A+    +E+ P I
Sbjct: 162 VEKVLNEIRPYLAGTGGGGLQFLMIKGPIVKVRLTGPAAVVRTVRIAVSKKLREKIPSI 219

BLAST of CSPI07G18350 vs. Swiss-Prot
Match: NIFU3_ARATH (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.2e-17
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 1

Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  VV LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KETTPEAVNSHLDILRPAIRNY-GGSVEVISINGGDCLV 193
           ++K  + +  + Q  + E    E   E +   L  LRP +    GG +E++ I+G    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGVKAAIKER 210
           +  GP +    V+ A+ ++
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

BLAST of CSPI07G18350 vs. Swiss-Prot
Match: YUTI_BACSU (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) GN=yutI PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.3e-13
Identity = 37/70 (52.86%), Postives = 53/70 (75.71%), Query Frame = 1

Query: 79  VDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFG 138
           V  VL+ +RP+L+ DGG+ ++V V++G+V L+L+GACGSCPSST T+K GIER L E+  
Sbjct: 43  VQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLEEV- 102

Query: 139 DSVKEICQVY 149
             V E+ QV+
Sbjct: 103 PGVVEVEQVF 111

BLAST of CSPI07G18350 vs. TrEMBL
Match: A0A0A0KB98_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G412870 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 4.6e-119
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of CSPI07G18350 vs. TrEMBL
Match: A0A140B480_PRUPE (NFU domain protein 1 OS=Prunus persica var. persica GN=NFU1 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.3e-78
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT +GL KTP   S+ PA S QYP   + Q     S    F   AIRAS + S  + 
Sbjct: 1   MASLTATGLPKTPTPFSKIPATSHQYPQSVFVQRHRLTSQGSIFVKSAIRAS-NPSAAAE 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSA+K+ELT+ NVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKL GACGSCPS
Sbjct: 61  SSSPGLYSAKKYELTVPNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGD++K+I QVYDEE KETT EAVN HLDILRPAI+N+GGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDALKDIQQVYDEENKETTVEAVNGHLDILRPAIKNFGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           +S+ GGDC V Y GP+SIG+G+KAAIKE+FPDI NVVF+
Sbjct: 181 LSVEGGDCHVNYVGPDSIGSGIKAAIKEKFPDIVNVVFT 218

BLAST of CSPI07G18350 vs. TrEMBL
Match: M5XUC8_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011214mg PE=4 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.3e-78
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT +GL KTP   S+ PA S QYP   + Q     S    F   AIRAS + S  + 
Sbjct: 1   MASLTATGLPKTPTPFSKIPATSHQYPQSVFVQRHRLTSQGSIFVKSAIRAS-NPSAAAE 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSA+K+ELT+ NVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKL GACGSCPS
Sbjct: 61  SSSPGLYSAKKYELTVPNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGD++K+I QVYDEE KETT EAVN HLDILRPAI+N+GGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDALKDIQQVYDEENKETTVEAVNGHLDILRPAIKNFGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           +S+ GGDC V Y GP+SIG+G+KAAIKE+FPDI NVVF+
Sbjct: 181 LSVEGGDCHVNYVGPDSIGSGIKAAIKEKFPDIVNVVFT 218

BLAST of CSPI07G18350 vs. TrEMBL
Match: W9R6P7_9ROSA (NifU-like protein 1 OS=Morus notabilis GN=L484_018769 PE=4 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 3.3e-77
Identity = 152/219 (69.41%), Postives = 176/219 (80.37%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTT G+ K+P  +      S Q+P F   + +   S  +     AIRAS  +S   +
Sbjct: 1   MASLTTLGIPKSPTPSGDILLKSHQFPQFIPLKQRRITSQSKGLSKTAIRASNQSSTTGS 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSAQKF+LT  NVD VLEDVRPYLI+DGGNVDVVSVEDGV+SLKL GACGSCPS
Sbjct: 61  SSSPGLYSAQKFDLTAQNVDFVLEDVRPYLISDGGNVDVVSVEDGVISLKLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGD+VK+I QVYDEEPKET  EAVN HLDILRPAI+N+GGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDAVKDIRQVYDEEPKETAVEAVNGHLDILRPAIKNFGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           +S+ G DC VKY+GPESIG+G+KAAIKE+FPDI NVVF+
Sbjct: 181 LSVEGEDCFVKYKGPESIGSGIKAAIKEKFPDIVNVVFT 219

BLAST of CSPI07G18350 vs. TrEMBL
Match: B9RNA2_RICCO (Nitrogen fixation protein nifU, putative OS=Ricinus communis GN=RCOM_1345940 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 9.3e-72
Identity = 151/217 (69.59%), Postives = 172/217 (79.26%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASL  +   K P    + PAN+ +YP        + +  +      AI+A+ S +G   
Sbjct: 1   MASLAFTAPSKIPPYCPKIPANTHRYP------QSITMKTRVVLSKTAIKAT-SQTG--- 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           + SPGLYSAQKFELT  NVDLVLEDVRPYLIADGGNVDVVSVEDGV+SL+L GACGSCPS
Sbjct: 61  SQSPGLYSAQKFELTPENVDLVLEDVRPYLIADGGNVDVVSVEDGVISLQLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVK+I QV DEE KETTPEAVN HLDILRPAI+NYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKDIRQVNDEEVKETTPEAVNRHLDILRPAIKNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVV 218
           +SI  G+CLVKY GPESIG+G+KAAIKE+FP+ITNVV
Sbjct: 181 VSIESGECLVKYSGPESIGSGIKAAIKEKFPEITNVV 207

BLAST of CSPI07G18350 vs. TAIR10
Match: AT4G01940.1 (AT4G01940.1 NFU domain protein 1)

HSP 1 Score: 249.6 bits (636), Expect = 1.8e-66
Identity = 142/230 (61.74%), Postives = 169/230 (73.48%), Query Frame = 1

Query: 1   MASLTTS--GLLKTPLKNSQS----PANSLQYPPFA-YGQHQLPVSLKRAFPGRAIRASP 60
           MASL TS  G  +  +K+S +    P  S Q P F  +   +  +S    F   AI  S 
Sbjct: 2   MASLATSISGSFRILVKSSSTRNGFPVISDQNPSFVLFANKRRHISRTAIFHRSAISGSS 61

Query: 61  SNSGPSTTSS---PGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLK 120
                S  +S    GLYSAQ F+LT  NVDLVLEDVRP+LI+DGGNVDVVSVEDGVVSLK
Sbjct: 62  QGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLK 121

Query: 121 LVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPA 180
           L GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV+DEE K+ T EAVN+HLDILRPA
Sbjct: 122 LQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEVKQITVEAVNAHLDILRPA 181

Query: 181 IRNYGGSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           I+NYGGSVEV+S+ G DC+VKY GPESIG G++AAIKE+F DI+NV F+S
Sbjct: 182 IKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKDISNVTFTS 231

BLAST of CSPI07G18350 vs. TAIR10
Match: AT5G49940.1 (AT5G49940.1 NIFU-like protein 2)

HSP 1 Score: 98.6 bits (244), Expect = 5.1e-21
Identity = 57/148 (38.51%), Postives = 84/148 (56.76%), Query Frame = 1

Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRP-AIRNYGGSVEVISINGGDCLVKY 193
            EK  + V       +E   E   E +   L+ +RP  I    GS++++ I      ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGVKAAIKERFPDITNV 217
            GP     ++   V   ++E+ P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

BLAST of CSPI07G18350 vs. TAIR10
Match: AT4G25910.1 (AT4G25910.1 NFU domain protein 3)

HSP 1 Score: 90.1 bits (222), Expect = 1.8e-18
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 1

Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  VV LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KETTPEAVNSHLDILRPAIRNY-GGSVEVISINGGDCLV 193
           ++K  + +  + Q  + E    E   E +   L  LRP +    GG +E++ I+G    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGVKAAIKER 210
           +  GP +    V+ A+ ++
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

BLAST of CSPI07G18350 vs. TAIR10
Match: AT3G20970.1 (AT3G20970.1 NFU domain protein 4)

HSP 1 Score: 54.7 bits (130), Expect = 8.5e-08
Identity = 32/85 (37.65%), Postives = 50/85 (58.82%), Query Frame = 1

Query: 75  TIGNVDLVLED-VRPYLIADGGNVDVVSV--EDGVVSLKLVGACGSCPSSTTTMKMGIER 134
           T+  +  +LE  +RP +  DGG+++      E G+V L++ GAC  CPSS+ T+K GIE 
Sbjct: 192 TVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSSSVTLKSGIEN 251

Query: 135 VLKEKFGDSVKEICQVYDEEPKETT 157
           +L   +   VK + Q +D E +E T
Sbjct: 252 MLMH-YVSEVKGVEQEFDGEDEEGT 275

BLAST of CSPI07G18350 vs. TAIR10
Match: AT1G51390.1 (AT1G51390.1 NFU domain protein 5)

HSP 1 Score: 53.1 bits (126), Expect = 2.5e-07
Identity = 31/85 (36.47%), Postives = 50/85 (58.82%), Query Frame = 1

Query: 75  TIGNVDLVLED-VRPYLIADGGNVDVVS--VEDGVVSLKLVGACGSCPSSTTTMKMGIER 134
           T+  +  +LE  +RP +  DGG+++      E G+V L++ GAC  CPSS+ T+K GIE 
Sbjct: 187 TVAMIKELLETRIRPSVQDDGGDIEYCGFDTETGIVKLRMQGACSGCPSSSVTLKSGIEN 246

Query: 135 VLKEKFGDSVKEICQVYDEEPKETT 157
           +L   +   VK + Q +D E + T+
Sbjct: 247 MLMH-YVSEVKGVEQEFDGEEEGTS 270

BLAST of CSPI07G18350 vs. NCBI nr
Match: gi|449436401|ref|XP_004135981.1| (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 435.3 bits (1118), Expect = 6.5e-119
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of CSPI07G18350 vs. NCBI nr
Match: gi|659101131|ref|XP_008451442.1| (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo])

HSP 1 Score: 426.4 bits (1095), Expect = 3.0e-116
Identity = 214/220 (97.27%), Postives = 218/220 (99.09%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKNSQSPAN  QYPPFAYGQHQLPVSLKRAFPGRA+RASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGG+CLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

BLAST of CSPI07G18350 vs. NCBI nr
Match: gi|596109907|ref|XP_007221501.1| (hypothetical protein PRUPE_ppa011214mg [Prunus persica])

HSP 1 Score: 300.1 bits (767), Expect = 3.3e-78
Identity = 158/219 (72.15%), Postives = 179/219 (81.74%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT +GL KTP   S+ PA S QYP   + Q     S    F   AIRAS + S  + 
Sbjct: 1   MASLTATGLPKTPTPFSKIPATSHQYPQSVFVQRHRLTSQGSIFVKSAIRAS-NPSAAAE 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSA+K+ELT+ NVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKL GACGSCPS
Sbjct: 61  SSSPGLYSAKKYELTVPNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGD++K+I QVYDEE KETT EAVN HLDILRPAI+N+GGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDALKDIQQVYDEENKETTVEAVNGHLDILRPAIKNFGGSVEV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           +S+ GGDC V Y GP+SIG+G+KAAIKE+FPDI NVVF+
Sbjct: 181 LSVEGGDCHVNYVGPDSIGSGIKAAIKEKFPDIVNVVFT 218

BLAST of CSPI07G18350 vs. NCBI nr
Match: gi|1009148079|ref|XP_015891751.1| (PREDICTED: nifU-like protein 1, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 297.0 bits (759), Expect = 2.8e-77
Identity = 156/219 (71.23%), Postives = 174/219 (79.45%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT +GL KTP  +      + QYP F   +       K  F   AI AS  +S P+ 
Sbjct: 1   MASLTATGLTKTPTPSQNIGGKTHQYPQFLSPKQLGLTCQKTIFLKTAINASNPSSAPTG 60

Query: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSPGLYSAQ FELT  NVDLVLEDVRPYLIADGGNVDVVSVE+GVVSLKL GACGSCPS
Sbjct: 61  SSSPGLYSAQTFELTARNVDLVLEDVRPYLIADGGNVDVVSVEEGVVSLKLQGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGD+VK+I QV +EE KETT EAVN HLDILRPAI+NYGGSV+V
Sbjct: 121 STTTMKMGIERVLKEKFGDAVKDIRQVEEEEQKETTAEAVNGHLDILRPAIKNYGGSVQV 180

Query: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           +S+ GGDC VKY GPESIG+G+KAAIKERFPDI NVVF+
Sbjct: 181 LSVEGGDCFVKYLGPESIGSGIKAAIKERFPDIQNVVFT 219

BLAST of CSPI07G18350 vs. NCBI nr
Match: gi|470104310|ref|XP_004288551.1| (PREDICTED: nifU-like protein 1, chloroplastic [Fragaria vesca subsp. vesca])

HSP 1 Score: 296.6 bits (758), Expect = 3.6e-77
Identity = 156/224 (69.64%), Postives = 184/224 (82.14%), Query Frame = 1

Query: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSN----- 60
           MASLTTS L KTP+ + Q PA S Q P F      + V   R F G ++RAS S+     
Sbjct: 1   MASLTTSWLPKTPIPSPQIPAKSHQSPQF------MLVKRTRIFLGSSVRASNSSAAAES 60

Query: 61  SGPSTTSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGAC 120
           S  S++SSPGLYSA++F+LT+ NVD+VLEDVRPYLIADGGNVDVVSVEDGVVSLKL GAC
Sbjct: 61  SSSSSSSSPGLYSAKQFDLTVPNVDMVLEDVRPYLIADGGNVDVVSVEDGVVSLKLQGAC 120

Query: 121 GSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYG 180
           GSCPSSTTTMKMGIERVLKEKFGD++K+I QV+DEE +ETT EAVN HLDILRPAI+N+G
Sbjct: 121 GSCPSSTTTMKMGIERVLKEKFGDALKDIQQVFDEENRETTVEAVNGHLDILRPAIKNFG 180

Query: 181 GSVEVISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           GSVEV+S+ GGDC VKY GP+SIG+G++AAIKE+FPDI NVVF+
Sbjct: 181 GSVEVLSVEGGDCHVKYVGPDSIGSGIRAAIKEKFPDIVNVVFT 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NIFU1_ARATH3.2e-6561.74NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana GN=NIFU1 PE=1 SV=1[more]
NIFU2_ARATH9.1e-2038.51NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana GN=NIFU2 PE=1 SV=1[more]
NIFU1_ORYSJ1.2e-1939.11NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica GN=NIFU1 PE=1... [more]
NIFU3_ARATH3.2e-1740.29NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana GN=NIFU3 PE=2 SV=1[more]
YUTI_BACSU6.3e-1352.86Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) GN=yut... [more]
Match NameE-valueIdentityDescription
A0A0A0KB98_CUCSA4.6e-119100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G412870 PE=4 SV=1[more]
A0A140B480_PRUPE2.3e-7872.15NFU domain protein 1 OS=Prunus persica var. persica GN=NFU1 PE=2 SV=1[more]
M5XUC8_PRUPE2.3e-7872.15Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011214mg PE=4 SV=1[more]
W9R6P7_9ROSA3.3e-7769.41NifU-like protein 1 OS=Morus notabilis GN=L484_018769 PE=4 SV=1[more]
B9RNA2_RICCO9.3e-7269.59Nitrogen fixation protein nifU, putative OS=Ricinus communis GN=RCOM_1345940 PE=... [more]
Match NameE-valueIdentityDescription
AT4G01940.11.8e-6661.74 NFU domain protein 1[more]
AT5G49940.15.1e-2138.51 NIFU-like protein 2[more]
AT4G25910.11.8e-1840.29 NFU domain protein 3[more]
AT3G20970.18.5e-0837.65 NFU domain protein 4[more]
AT1G51390.12.5e-0736.47 NFU domain protein 5[more]
Match NameE-valueIdentityDescription
gi|449436401|ref|XP_004135981.1|6.5e-119100.00PREDICTED: nifU-like protein 1, chloroplastic [Cucumis sativus][more]
gi|659101131|ref|XP_008451442.1|3.0e-11697.27PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo][more]
gi|596109907|ref|XP_007221501.1|3.3e-7872.15hypothetical protein PRUPE_ppa011214mg [Prunus persica][more]
gi|1009148079|ref|XP_015891751.1|2.8e-7771.23PREDICTED: nifU-like protein 1, chloroplastic [Ziziphus jujuba][more]
gi|470104310|ref|XP_004288551.1|3.6e-7769.64PREDICTED: nifU-like protein 1, chloroplastic [Fragaria vesca subsp. vesca][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001075NIF_FeS_clus_asmbl_NifU_C
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0051536iron-sulfur cluster binding
Vocabulary: Biological Process
TermDefinition
GO:0016226iron-sulfur cluster assembly
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0008152 metabolic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0005575 cellular_component
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0005198 structural molecule activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G18350.1CSPI07G18350.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPRODOMPD002830coord: 83..135
score: 9.0
IPR001075NIF system FeS cluster assembly, NifU, C-terminalPFAMPF01106NifUcoord: 161..216
score: 8.0E-5coord: 80..139
score: 8.0
NoneNo IPR availablePANTHERPTHR11178IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATEDcoord: 65..211
score: 5.6
NoneNo IPR availableunknownSSF117916Fe-S cluster assembly (FSCA) domain-likecoord: 77..149
score: 3.49E-21coord: 155..217
score: 7.8

The following gene(s) are paralogous to this gene:

None