CSPI06G04020 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G04020
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	F1F0-ATPase inhibitor protein
Location	Chr6 : 3736501 .. 3738344 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGGCTCAATTAGTGTTAGGGCTATAAAAATGAACCGACGGAACCATGCTGTCACTCTCTAGAACATTTCGATTTCCATAAGCTCAAATGGCCATGCGATCGGTGCTCACTCGATCATCGTTGACTACTTCGACGGCGCGTGCCGGTGGATTCCGCTTCCTCTCCGACGGCAAAGGTCGAGTGCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGGTTGAATTATTAGTTTCGAATGTTCGAGAAAAGTTTGTAGAAACCAGTAGAACGCCGAGTGTCGACCGGTTTTTGTAATTTTCAGTTGTTACAAACTCAGATGGAATGGTTTAGACTCTAGAGCGAGAGCGAAGTTACTGTTTCGATACTTTCTTTTTTTGTGCTCGGAATTTGCTTTCTCTGATTTATCGCGGAGTATGGTCTTCAATCTGTTTGACCGAATACACACTTGTTCGAAACTCTAATAAAAATGAACTGGAGTGGGAGCATGTTTACTGTTTATGTCAGATGGTATTTGTGAGGAATTTGTGATCTCTGATTTACTGCAGTAGGAGTTGGCTTGGCGAATTTCCATTTTCTCGAAACTTTAATAGAAATGCACAAGACTGTAAATGAAGTTATGGAATCTATGTTCTCCGATTTTGAGTTATTGTTTTTCTAATGTTTGTAGAAAATGGAAAGAGAAAGGTTGGAGAAGCTGAGGCTTAAGGCAGAGAAAGAAAAGGCTCAGAAGGAGAAAGAACAGTCTGAGAAGGTATTCGTCACTCTTATAATTTTGGTTGAAATTGTCGAATATTGTTTATTAGTTGATTTGTGATATGTATGACAGTGAAAAACTGTGTTTTGTGATTATGCAACATTTCTTTTCATCATGCTTTATGGCTTTGTGGGATTTTGGATCCATCGTCATCCTTTGTTTGTTCCCAACTCTTTGCTTGAGTAGCTTGCACTGACTATTTGTTCGATCAGTATTTGATTTCTTGTATCTATATGGAAATTGAGCCCAAGCTTCTGATAAATAGATATCGATTGCAAGATATTATGGTCAGCTTTTGCTTGTGTCCAATCCAATTATGGTTCATTAGGAACTACGATTTTCATAGCGGCTGAAATTTAATGGGATTTAATCCTTGAATCTTCGTTTCTTGTTTCCTGCAGAAATTTGTTACCACTATTAAAGTATTCTGTAAATAAAGCCCGAATCTAGAATGCTGTTATGGCAGTATGCCGAAGCACCTTTTTGTTCCTTATCTCTTTCTTTCGGTGCTTCGTGTCTCTAGTCATCAGACTGATCGGTTTATACTTGAAATATGACAAAGTTTTGGTACTACTCTTGTGATATGACAAACTTTCTCACTAATTTTTGTACTTTTACCATTTCCCAAACAAATTTACAAGTTGAGTTATTAAATTCCACTTCAGGAGTAGCATATCTATCTTCTAAGGAGTGTGAGGCCTTTTGGTTTTTCTTTTTTCAATGTGGGTTCTCAACAGATCTCTCAAGATGATGTCCCTTTTGTGTTTACCATTCTTTTTTATTGGACCTAATACTTGCCCATATTAGATAATATGGGTTGACATGAGATTCTCCTCCGCTTGATAATGATGATGTCTCACTATATCTATAAGGAAAAAGTTGTAATGTCATAATTTAAATGTTTCAATATTAACTTAACTCTCGTTTCTCATATTGTAAACTTTGTTTACCCTTTTTCTCATCATTGTTTCATATCTGCAGAAACATGAAGGGACCCAGTAGAAAAAGACCGTGGGCACATCTGCTTTGAAGTTTGATCTTGATCGCATGGAATAAAATGTGAGATTTCACCCCCCCCC

mRNA sequence

ATGGCCATGCGATCGGTGCTCACTCGATCATCGTTGACTACTTCGACGGCGCGTGCCGGTGGATTCCGCTTCCTCTCCGACGGCAAAGGTCGAGTGCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGAAAATGGAAAGAGAAAGGTTGGAGAAGCTGAGGCTTAAGGCAGAGAAAGAAAAGGCTCAGAAGGAGAAAGAACAGTCTGAGAAGAAACATGAAGGGACCCAGTAG

Coding sequence (CDS)

ATGGCCATGCGATCGGTGCTCACTCGATCATCGTTGACTACTTCGACGGCGCGTGCCGGTGGATTCCGCTTCCTCTCCGACGGCAAAGGTCGAGTGCTTAGTGAGGAGGAGCGTGCTGCCGAGAACGTCTACATCCAGAAAATGGAAAGAGAAAGGTTGGAGAAGCTGAGGCTTAAGGCAGAGAAAGAAAAGGCTCAGAAGGAGAAAGAACAGTCTGAGAAGAAACATGAAGGGACCCAGTAG

Homology

[Close]

BLAST of CSPI06G04020 vs. TrEMBL
Match: A0A0A0K8J7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G046230 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 5.5e-30
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 1


Query: 1  MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA 60
          MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA
Sbjct: 1  MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA 60

Query: 61 EKEKAQKEKEQSEKKHEGTQ 81
          EKEKAQKEKEQSEKKHEGTQ
Sbjct: 61 EKEKAQKEKEQSEKKHEGTQ 80

[Close]

BLAST of CSPI06G04020 vs. TrEMBL
Match: B9HVV9_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s24670g PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 8.5e-15
Identity = 56/83 (67.47%), Postives = 66/83 (79.52%), Query Frame = 1


Query: 1  MAMRSVLTRSSLTT---STARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MA RS L+R SL T     +  G  R+ SDGKGRVLSEEERAAENVYIQKMERERLEKL+
Sbjct: 1  MATRSALSRLSLITIRSMKSTRGATRYFSDGKGRVLSEEERAAENVYIQKMERERLEKLK 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LK EKEKA+KEK+ ++K+ EG+Q
Sbjct: 61 LKQEKEKAEKEKQGADKRIEGSQ 83

[Close]

BLAST of CSPI06G04020 vs. TrEMBL
Match: W9QGS0_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_018375 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 3.2e-14
Identity = 53/76 (69.74%), Postives = 61/76 (80.26%), Query Frame = 1


Query: 1  MAMRSVLTRSSLTTSTARA--GGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRL 60
          MAMRSV+ R  + T +     G  R+LSDGKGR+LSEEERAAENVYIQKMERER EKL+ 
Sbjct: 1  MAMRSVVRRVGILTRSMETTRGATRYLSDGKGRILSEEERAAENVYIQKMERERSEKLKQ 60

Query: 61 KAEKEKAQKEKEQSEK 75
          KAEKEKAQKEKE ++K
Sbjct: 61 KAEKEKAQKEKENADK 76

[Close]

BLAST of CSPI06G04020 vs. TrEMBL
Match: A0A067LFT4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_26475 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.2e-13
Identity = 54/78 (69.23%), Postives = 61/78 (78.21%), Query Frame = 1


Query: 1  MAMRSVLTRSSLTTST-ARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLK 60
          MA R  ++R  +T S  +  G  R+ SDGKGRVLSEEERAAENVYIQKMERERLEKL+ K
Sbjct: 1  MATRLAISRLGITRSMESTRGATRYFSDGKGRVLSEEERAAENVYIQKMERERLEKLKQK 60

Query: 61 AEKEKAQKEKEQSEKKHE 78
          AEKEKA KEKE +EKK E
Sbjct: 61 AEKEKAAKEKESAEKKTE 78

[Close]

BLAST of CSPI06G04020 vs. TrEMBL
Match: A0A067E0M2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g034789mg PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.2e-13
Identity = 56/78 (71.79%), Postives = 60/78 (76.92%), Query Frame = 1


Query: 1  MAMRSVLTRSSLTTST-ARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLK 60
          MAMRS L R  +T S  +  G  R+ SD KGRVLSEEERAAENVYIQKMERERLEK R K
Sbjct: 1  MAMRSALCRLKVTRSMESTRGATRYFSDDKGRVLSEEERAAENVYIQKMERERLEKQRKK 60

Query: 61 AEKEKAQKEKEQSEKKHE 78
          AEKEK QKEKE +EKK E
Sbjct: 61 AEKEKLQKEKEGAEKKTE 78

[Close]

BLAST of CSPI06G04020 vs. TAIR10
Match: AT5G04750.1 (AT5G04750.1 F1F0-ATPase inhibitor protein, putative)

HSP 1 Score: 52.8 bits (125), Expect = 1.2e-07
Identity = 37/68 (54.41%), Postives = 47/68 (69.12%), Query Frame = 1


Query: 10 SSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKAEKEKAQKEK 69
          +S +  + R    R+ SD KGRVLSEEERA E++YIQKMERERLEK + K E++K   EK
Sbjct: 24 ASRSVVSTRGPAIRYFSDDKGRVLSEEERAKESMYIQKMERERLEK-KKKLEQDKLDGEK 83

Query: 70 EQSEKKHE 78
            + KK E
Sbjct: 84 GSANKKPE 90

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: gi|449446299|ref|XP_004140909.1| (PREDICTED: uncharacterized protein At2g27730, mitochondrial [Cucumis sativus])

HSP 1 Score: 137.9 bits (346), Expect = 7.9e-30
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 1


Query: 1  MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA 60
          MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA
Sbjct: 1  MAMRSVLTRSSLTTSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRLKA 60

Query: 61 EKEKAQKEKEQSEKKHEGTQ 81
          EKEKAQKEKEQSEKKHEGTQ
Sbjct: 61 EKEKAQKEKEQSEKKHEGTQ 80

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: gi|659113500|ref|XP_008456607.1| (PREDICTED: uncharacterized protein LOC103496516 [Cucumis melo])

HSP 1 Score: 125.6 bits (314), Expect = 4.1e-26
Identity = 76/83 (91.57%), Postives = 78/83 (93.98%), Query Frame = 1


Query: 1  MAMRSVLTRSSLTTSTARA---GGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MAMRSVL+RSSLT  TARA   GGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR
Sbjct: 1  MAMRSVLSRSSLTPCTARAAGAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LKAEKEKA+KEKEQSEKKHEGTQ
Sbjct: 61 LKAEKEKAEKEKEQSEKKHEGTQ 83

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: gi|1009119695|ref|XP_015876519.1| (PREDICTED: uncharacterized protein At2g27730, mitochondrial-like [Ziziphus jujuba])

HSP 1 Score: 88.2 bits (217), Expect = 7.2e-15
Identity = 59/81 (72.84%), Postives = 66/81 (81.48%), Query Frame = 1


Query: 1  MAMRS-VLTRSSLT-TSTARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRL 60
          MAMRS VL+R+ LT T  +  G  R+ SD KGRVLSEEERAAEN+YIQKMERERLEK +L
Sbjct: 1  MAMRSLVLSRAVLTRTMESTRGPTRYFSDSKGRVLSEEERAAENIYIQKMERERLEKQKL 60

Query: 61 KAEKEKAQKEKEQSEKKHEGT 80
          KAEKEKA+KEKE S KK EGT
Sbjct: 61 KAEKEKAEKEKENSGKKPEGT 81

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: gi|224113431|ref|XP_002316494.1| (hypothetical protein POPTR_0010s24670g [Populus trichocarpa])

HSP 1 Score: 87.4 bits (215), Expect = 1.2e-14
Identity = 56/83 (67.47%), Postives = 66/83 (79.52%), Query Frame = 1


Query: 1  MAMRSVLTRSSLTT---STARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLR 60
          MA RS L+R SL T     +  G  R+ SDGKGRVLSEEERAAENVYIQKMERERLEKL+
Sbjct: 1  MATRSALSRLSLITIRSMKSTRGATRYFSDGKGRVLSEEERAAENVYIQKMERERLEKLK 60

Query: 61 LKAEKEKAQKEKEQSEKKHEGTQ 81
          LK EKEKA+KEK+ ++K+ EG+Q
Sbjct: 61 LKQEKEKAEKEKQGADKRIEGSQ 83

[Close]

BLAST of CSPI06G04020 vs. NCBI nr
Match: gi|1009165502|ref|XP_015901073.1| (PREDICTED: uncharacterized protein At2g27730, mitochondrial-like [Ziziphus jujuba])

HSP 1 Score: 86.7 bits (213), Expect = 2.1e-14
Identity = 58/81 (71.60%), Postives = 66/81 (81.48%), Query Frame = 1


Query: 1  MAMRS-VLTRSSLTTST-ARAGGFRFLSDGKGRVLSEEERAAENVYIQKMERERLEKLRL 60
          MAMRS VL+R+ LT +  +  G  R+ SD KGRVLSEEERAAEN+YIQKMERERLEK +L
Sbjct: 1  MAMRSLVLSRAVLTRAMESTRGPTRYFSDSKGRVLSEEERAAENIYIQKMERERLEKQKL 60

Query: 61 KAEKEKAQKEKEQSEKKHEGT 80
          KAEKEKA+KEKE S KK EGT
Sbjct: 61 KAEKEKAEKEKENSGKKPEGT 81

The following BLAST results are available for this feature:

BLAST of CSPI06G04020 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI06G04020 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8J7_CUCSA	5.5e-30	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G046230 PE=4 SV=1	[more]
B9HVV9_POPTR	8.5e-15	67.47	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0010s24670g PE=4 SV=1	[more]
W9QGS0_9ROSA	3.2e-14	69.74	Uncharacterized protein OS=Morus notabilis GN=L484_018375 PE=4 SV=1	[more]
A0A067LFT4_JATCU	1.2e-13	69.23	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_26475 PE=4 SV=1	[more]
A0A067E0M2_CITSI	1.2e-13	71.79	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g034789mg PE=4 SV=1	[more]

BLAST of CSPI06G04020 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G04750.1	1.2e-07	54.41	F1F0-ATPase inhibitor protein, putative	[more]

BLAST of CSPI06G04020 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449446299\|ref\|XP_004140909.1\|	7.9e-30	100.00	PREDICTED: uncharacterized protein At2g27730, mitochondrial [Cucumis sativus]	[more]
gi\|659113500\|ref\|XP_008456607.1\|	4.1e-26	91.57	PREDICTED: uncharacterized protein LOC103496516 [Cucumis melo]	[more]
gi\|1009119695\|ref\|XP_015876519.1\|	7.2e-15	72.84	PREDICTED: uncharacterized protein At2g27730, mitochondrial-like [Ziziphus jujub...	[more]
gi\|224113431\|ref\|XP_002316494.1\|	1.2e-14	67.47	hypothetical protein POPTR_0010s24670g [Populus trichocarpa]	[more]
gi\|1009165502\|ref\|XP_015901073.1\|	2.1e-14	71.60	PREDICTED: uncharacterized protein At2g27730, mitochondrial-like [Ziziphus jujub...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR007648	ATPase_inhibitor_mt

Vocabulary: **Cellular Component**
Term	Definition
GO:0005739	mitochondrion

Vocabulary: **Biological Process**
Term	Definition
GO:0032780	negative regulation of ATPase activity

Vocabulary: **Molecular Function**
Term	Definition
GO:0042030	ATPase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032780	negative regulation of ATPase activity
biological_process	GO:0045980	negative regulation of nucleotide metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0042030	ATPase inhibitor activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G04020.1	CSPI06G04020.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007648	Mitochondrial ATPase inhibitor	PFAM	PF04568	IATP	coord: 27..74 score: 1.
None	No IPR available	unknown	Coil	Coil	coord: 45..80 scor
None	No IPR available	PANTHER	PTHR33878	FAMILY NOT NAMED	coord: 2..80 score: 9.0
None	No IPR available	PANTHER	PTHR33878:SF2	SUBFAMILY NOT NAMED	coord: 2..80 score: 9.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI06G04020	Cla013688	Watermelon (97103) v1	cpiwmB472
CSPI06G04020	ClCG08G008330	Watermelon (Charleston Gray)	cpiwcgB523
CSPI06G04020	CsaV3_6G004310	Cucumber (Chinese Long) v3	cpicucB364
CSPI06G04020	Bhi04G001317	Wax gourd	cpiwgoB593
CSPI06G04020	Carg15855	Silver-seed gourd	carcpiB0070
CSPI06G04020	Carg01673	Silver-seed gourd	carcpiB0870

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI06G04020	Cucurbita moschata (Rifu)	cmocpiB306
CSPI06G04020	Cucurbita moschata (Rifu)	cmocpiB769
CSPI06G04020	Cucumber (Chinese Long) v2	cpicuB314
CSPI06G04020	Melon (DHL92) v3.5.1	cpimeB473
CSPI06G04020	Cucurbita pepo (Zucchini)	cpecpiB198
CSPI06G04020	Cucurbita pepo (Zucchini)	cpecpiB460
CSPI06G04020	Bottle gourd (USVL1VR-Ls)	cpilsiB487
CSPI06G04020	Melon (DHL92) v3.6.1	cpimedB465
CSPI06G04020	Cucumber (Gy14) v2	cgybcpiB288
CSPI06G04020	Watermelon (97103) v2	cpiwmbB514
CSPI06G04020	Cucumber (Gy14) v1	cgycpiB540
CSPI06G04020	Cucurbita maxima (Rimu)	cmacpiB315
CSPI06G04020	Cucurbita maxima (Rimu)	cmacpiB778