CSPI05G29960 (gene) Wild cucumber (PI 183967)

NameCSPI05G29960
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionUnknown protein
LocationChr5 : 28018765 .. 28019539 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTGTAATGGTTTGTTGTATATCTGCAAGTTTATTTTTGACTCTCTATGTGAAGGCATATTAAATCCGATGACAAATGAATTTGTGGAAATTCCTCCACCCGATATAGAATTAGATGTTCACTGCGTTGGTTTTGGTTTCAGCCCTGAACAAAACAATACAAACTATTCAGAACTGAACCAGAAATCCTTCCCAATTGGAAAAAGTGCCATTACAGAATGGATATTTTTACCTTTGATAATGGCCATAAGCAATGGAGGCATTTTAAGCGTCTACCTTTTGTAGTCATCCATCATGGTCAGTACTTGAATGGAATCATCTACTGGATCGGAAAGAAATTAGAAAAAGAGGGGGAGGTTGTGATATATGCTCTCCATGTCGACACTGAACAGATGGAATGCATTGCAACCTTAAAAATTGGAGCTTTCTTGAAGAATGGTTCCATTAAGATATACGAGGACAGGGTTTACGCAGTTATTAACTTGTTGGACAAACGAGTATCTCCACCATGTTATAAGATTGAATTGTGGAGGATGCAAAAGAAAGATTCATGGATTAAAGAATTTAGTGCTGATGAAGAATTTTTTGGCTTTGTTGAAGTTTTTGAAGATGGAGAGTTGTTGTTTGCGATCAAAGATGACTTACTTCGTTTGGATAAGAGTAGAAAGAAGATCAAGATCAAAACATTGAATCCAAGTGAGAGTACAACTAAAGCTACAGGGCGTTATACTATAAAATCTATTAATTTCGAGTCTCTTTCAACCATTTTGGTTTGA

mRNA sequence

ATGGATATTTTTACCTTTGATAATGGCCATAAGCAATGGAGGCATTTTAAGCGTCTACCTTTTGTAGTCATCCATCATGGTCAGTACTTGAATGGAATCATCTACTGGATCGGAAAGAAATTAGAAAAAGAGGGGGAGGTTGTGATATATGCTCTCCATGTCGACACTGAACAGATGGAATGCATTGCAACCTTAAAAATTGGAGCTTTCTTGAAGAATGGTTCCATTAAGATATACGAGGACAGGGTTTACGCAGTTATTAACTTGTTGGACAAACGAGTATCTCCACCATGTTATAAGATTGAATTGTGGAGGATGCAAAAGAAAGATTCATGGATTAAAGAATTTAGTGCTGATGAAGAATTTTTTGGCTTTGTTGAAGTTTTTGAAGATGGAGAGTTGTTGTTTGCGATCAAAGATGACTTACTTCGTTTGGATAAGAGTAGAAAGAAGATCAAGATCAAAACATTGAATCCAAGTGAGAGTACAACTAAAGCTACAGGGCGTTATACTATAAAATCTATTAATTTCGAGTCTCTTTCAACCATTTTGGTTTGA

Coding sequence (CDS)

ATGGATATTTTTACCTTTGATAATGGCCATAAGCAATGGAGGCATTTTAAGCGTCTACCTTTTGTAGTCATCCATCATGGTCAGTACTTGAATGGAATCATCTACTGGATCGGAAAGAAATTAGAAAAAGAGGGGGAGGTTGTGATATATGCTCTCCATGTCGACACTGAACAGATGGAATGCATTGCAACCTTAAAAATTGGAGCTTTCTTGAAGAATGGTTCCATTAAGATATACGAGGACAGGGTTTACGCAGTTATTAACTTGTTGGACAAACGAGTATCTCCACCATGTTATAAGATTGAATTGTGGAGGATGCAAAAGAAAGATTCATGGATTAAAGAATTTAGTGCTGATGAAGAATTTTTTGGCTTTGTTGAAGTTTTTGAAGATGGAGAGTTGTTGTTTGCGATCAAAGATGACTTACTTCGTTTGGATAAGAGTAGAAAGAAGATCAAGATCAAAACATTGAATCCAAGTGAGAGTACAACTAAAGCTACAGGGCGTTATACTATAAAATCTATTAATTTCGAGTCTCTTTCAACCATTTTGGTTTGA
BLAST of CSPI05G29960 vs. TrEMBL
Match: A0A0A0KUJ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643280 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-26
Identity = 77/183 (42.08%), Postives = 106/183 (57.92%), Query Frame = 1

Query: 11  KQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEGEVVIYALHVDTEQMECIATLKIGA- 70
           ++WRHFK  P     HG YLNG+IYW+GK  EK    VIYAL V+TEQME +A L++G  
Sbjct: 123 EEWRHFKCPPISFDDHGAYLNGVIYWMGK--EKGKAYVIYALDVETEQMELVADLEVGPH 182

Query: 71  -FLKNGSIKIYEDRVYAVINLLDKRVSPPCYKIELWRMQKKDSWIKEFSADEEF------ 130
            F  NG I      VYA + +    V P C KI++W MQ K+ WI+EF   +E       
Sbjct: 183 NFRYNGYIATLNKSVYAYVPI----VGPCCTKIQIWTMQGKEGWIREFVMYDEVSRGLRM 242

Query: 131 -FGFVEVFEDGELLFAIKDDLLRLDKSRKKIKIKTLNPSESTTKATGRYTIKSINFESLS 185
               V+V +DGE  F ++ ++L  DK+   IKI++L  S+  T+  G   I+S+NF S+ 
Sbjct: 243 PMQMVKVLKDGERWFFVEGNILCCDKTGMNIKIESLWNSKIETRFDGLCQIESLNFGSIQ 299

BLAST of CSPI05G29960 vs. TrEMBL
Match: A0A0A0KSN0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643960 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.5e-19
Identity = 44/45 (97.78%), Postives = 44/45 (97.78%), Query Frame = 1

Query: 1   MDIFTFDNGHKQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEG 46
           MDIFTFDNGHKQWRHFKRLPFVV HHGQYLNGIIYWIGKKLEKEG
Sbjct: 120 MDIFTFDNGHKQWRHFKRLPFVVFHHGQYLNGIIYWIGKKLEKEG 164

BLAST of CSPI05G29960 vs. TrEMBL
Match: A0A0A0KSJ2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643290 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.7e-18
Identity = 74/201 (36.82%), Postives = 100/201 (49.75%), Query Frame = 1

Query: 1   MDIFTFDNGH--KQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEGEVVIYALHVDTEQ 60
           M++  F      K+WRH   LPFV    G YLNG+IYWIG+  +KE + VIY+L+V+TE+
Sbjct: 193 MEVMRFGKNEETKEWRHLTCLPFVFYCDGVYLNGVIYWIGR--QKENKSVIYSLNVETEK 252

Query: 61  MECIATLKIGAFLKNGS----IKIYEDRVYAVINLLDKRVSPPCYKIELWRMQKKDSWIK 120
           +E I  LK+            I  Y   +YA I +        C K++LW MQ KD W+K
Sbjct: 253 IESITVLKVDPSYNTSDYGYYIGKYNGNLYATIWI----HGTCCKKVQLWMMQGKDCWVK 312

Query: 121 EFSAD-------EEFFGFVEVFEDGELLFAIKDDL-LRLDKSRKKIKIK--TLNPSESTT 180
           EF          + F   V++FEDGE  F +   L L  DK+  +IK K      S    
Sbjct: 313 EFVVHDIEDRYVDAFLVLVKIFEDGERWFYVGSRLILCYDKTGTQIKKKCRMKRVSNGRG 372

Query: 181 KATGRYT-IKSINFESLSTIL 185
              G+     S+NF SL  IL
Sbjct: 373 NLVGQLCHFDSLNFGSLPNIL 387

BLAST of CSPI05G29960 vs. TrEMBL
Match: A0A0A0LWG1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G257290 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 8.5e-18
Identity = 73/204 (35.78%), Postives = 110/204 (53.92%), Query Frame = 1

Query: 1   MDIFTFDNG--------HKQWRHFKRLPFVVIHHGQYLNGIIYWIGKK--LEKEGEVVIY 60
           M++ TF           H QWR    LPF ++  G YLNGIIYW+ K+   +KE + VIY
Sbjct: 212 MEVLTFGRSGTNHSIPIHNQWRRLHTLPFEIVADGVYLNGIIYWLVKEKGKDKEKKYVIY 271

Query: 61  ALHVDTEQMECIATLKIGAFLKNGSIKIYE--DRVYAVINLLDKRVSPPCYKIELWRMQK 120
           AL V+TE +E    L++ +   NG  KI++    +YA  ++  ++ S     I++WRMQ+
Sbjct: 272 ALDVETEHIEMTVVLQVPS---NGYGKIHQFNGTIYATFHINWEKDS---RTIQVWRMQE 331

Query: 121 KDSWIKEF-----SADEEFFGFVEVFEDGELLFAIKDDLLRL-DKSRKKIKIKTLNPSES 180
           KDSW+++F     S +      +++FED E+LF I  D     + S KK +I + N  + 
Sbjct: 332 KDSWVRKFVICDISREWIHLQLIKMFEDEEILFMINMDFFCFYNPSSKKKRIISKNQKKK 391

Query: 181 TTKATGRY--TIKSINFESLSTIL 185
                 RY   I+S+NF  LS IL
Sbjct: 392 ------RYICQIESLNFGRLSQIL 403

BLAST of CSPI05G29960 vs. TrEMBL
Match: A0A0A0LPC6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G014420 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.1e-16
Identity = 71/200 (35.50%), Postives = 104/200 (52.00%), Query Frame = 1

Query: 1   MDIFTFDNG-------HKQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEG--EVVIYA 60
           M++ TF          H QWRH   L   +IH G YLNGIIYW+GK+  K+   E VIYA
Sbjct: 233 MEVLTFGRSGTNHIPIHNQWRHLHNLSCDLIH-GVYLNGIIYWLGKEKGKKEKKEYVIYA 292

Query: 61  LHVDTEQMECIATLKIGAFLKNGSIKIYEDRVYAVINLLDKRVSPPCYKIELWRMQKKDS 120
           L V+TEQ+E    L++ A   +G +  +   +YA+  +  ++ S     I++W MQ+K S
Sbjct: 293 LDVETEQIEMSVILQVQA--SDGKMHPFNGTIYALFYINWEKDSRT---IQVWSMQEKCS 352

Query: 121 WIKEF-----SADEEFFGFVEVFEDGELLFAIKDDLLRL--DKSRKKIKIKTLNPSESTT 180
           WI++F     S +      +++FEDGE+LF I  D        + KK  I     S++  
Sbjct: 353 WIRQFVIRDISKEWGCLELIKIFEDGEILFLIDSDFFCFYHPLTEKKRII-----SKNQK 412

Query: 181 KATGRYTIKSINFESLSTIL 185
           K      I+ +NF SL  IL
Sbjct: 413 KNRFVCQIECLNFGSLPEIL 421

BLAST of CSPI05G29960 vs. NCBI nr
Match: gi|659119533|ref|XP_008459707.1| (PREDICTED: putative F-box protein At1g55070 [Cucumis melo])

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-77
Identity = 146/184 (79.35%), Postives = 159/184 (86.41%), Query Frame = 1

Query: 1   MDIFTFDNGHKQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEGEVVIYALHVDTEQME 60
           M+IFTFDNG+KQWRHF+RLPFVV HHGQYLNG+IYWIGKKLEKEGE VIYAL VDTEQME
Sbjct: 190 MEIFTFDNGYKQWRHFERLPFVVFHHGQYLNGVIYWIGKKLEKEGEAVIYALDVDTEQME 249

Query: 61  CIATLKIGAFLKNGSIKIYEDRVYAVINLLDKRVSPPCYKIELWRMQKKDSWIKEFSADE 120
           C ATL+IG  LKNGSIKI+E+RVYA+I+L+DKRVSP  YKIELWRMQKKDSWIKEFS DE
Sbjct: 250 CTATLEIGPSLKNGSIKIFEERVYAIISLMDKRVSPTSYKIELWRMQKKDSWIKEFSIDE 309

Query: 121 EFFGFVEVFEDGELLFAIKDDLLRLDKSRKKIKIKTLNPSESTTKATGRYTIKSINFESL 180
           E FG + VFEDGELL AIKD++ RLDKSRKKIKIKTLN SES  K  G  TI SINFE  
Sbjct: 310 ESFGLITVFEDGELLIAIKDNIFRLDKSRKKIKIKTLNSSESRIKVIGCNTINSINFECF 369

Query: 181 STIL 185
           S IL
Sbjct: 370 SNIL 373

BLAST of CSPI05G29960 vs. NCBI nr
Match: gi|449447691|ref|XP_004141601.1| (PREDICTED: F-box protein At3g07870-like [Cucumis sativus])

HSP 1 Score: 127.1 bits (318), Expect = 3.2e-26
Identity = 77/183 (42.08%), Postives = 106/183 (57.92%), Query Frame = 1

Query: 11  KQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEGEVVIYALHVDTEQMECIATLKIGA- 70
           ++WRHFK  P     HG YLNG+IYW+GK  EK    VIYAL V+TEQME +A L++G  
Sbjct: 123 EEWRHFKCPPISFDDHGAYLNGVIYWMGK--EKGKAYVIYALDVETEQMELVADLEVGPH 182

Query: 71  -FLKNGSIKIYEDRVYAVINLLDKRVSPPCYKIELWRMQKKDSWIKEFSADEEF------ 130
            F  NG I      VYA + +    V P C KI++W MQ K+ WI+EF   +E       
Sbjct: 183 NFRYNGYIATLNKSVYAYVPI----VGPCCTKIQIWTMQGKEGWIREFVMYDEVSRGLRM 242

Query: 131 -FGFVEVFEDGELLFAIKDDLLRLDKSRKKIKIKTLNPSESTTKATGRYTIKSINFESLS 185
               V+V +DGE  F ++ ++L  DK+   IKI++L  S+  T+  G   I+S+NF S+ 
Sbjct: 243 PMQMVKVLKDGERWFFVEGNILCCDKTGMNIKIESLWNSKIETRFDGLCQIESLNFGSIQ 299

BLAST of CSPI05G29960 vs. NCBI nr
Match: gi|659119531|ref|XP_008459706.1| (PREDICTED: uncharacterized protein LOC103498748 [Cucumis melo])

HSP 1 Score: 124.4 bits (311), Expect = 2.1e-25
Identity = 73/183 (39.89%), Postives = 108/183 (59.02%), Query Frame = 1

Query: 11  KQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEGEVVIYALHVDTEQMECIATLKIG-- 70
           ++WRHFK  P    +HG YLNG+IYW+GK  E+  E VIYAL V+TEQ+E +A L++G  
Sbjct: 209 EEWRHFKCPPISFDNHGAYLNGVIYWVGK--EEGKEHVIYALDVETEQIESVAVLEVGPH 268

Query: 71  AFLKNGSIKIYEDRVYAVINLLDKRVSPPCYKIELWRMQKKDSWIKEFSADEEF------ 130
           +F  NG I    + VYA + +    +   C ++++W MQ+K SWI+EF   +E       
Sbjct: 269 SFRYNGYITTLNNSVYAHVLI----IGTCCTRVQVWTMQEKKSWIREFVMHDEIPKSLIM 328

Query: 131 -FGFVEVFEDGELLFAIKDDLLRLDKSRKKIKIKTLNPSESTTKATGRYTIKSINFESLS 185
               V+V +DGE  F +  ++   DK+   IKIK+L  S+  T+  G   I+S+NF S+ 
Sbjct: 329 PMQLVKVLKDGERWFFVDGNIFCCDKTGMNIKIKSLWNSKIETRFDGLCQIESLNFGSIQ 385

BLAST of CSPI05G29960 vs. NCBI nr
Match: gi|659117583|ref|XP_008458678.1| (PREDICTED: uncharacterized protein LOC103498006 [Cucumis melo])

HSP 1 Score: 108.6 bits (270), Expect = 1.2e-20
Identity = 70/195 (35.90%), Postives = 105/195 (53.85%), Query Frame = 1

Query: 1   MDIFTFD-NGHKQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEGEVVIYALHVDTEQM 60
           M I  FD +G  + +    LPF +  HG YLNG IYWIG++  K    VIYAL V+TE++
Sbjct: 158 MQITKFDRHGTNRCKDIMCLPFNIDGHGSYLNGFIYWIGREQTK---FVIYALDVETEKI 217

Query: 61  ECIATLKIGAF-LKNGSIKIYEDRVYAVINLLDKRVSPPCYKIELWRMQKKDSWIKEFSA 120
           E   TL +G     +G I+     +Y  + + +     P + +++WRMQ KDSWI+ F  
Sbjct: 218 EWNTTLDVGTLSFCSGEIQTLNGSIYVTVFMYE-----PSHMLQVWRMQGKDSWIRTFVI 277

Query: 121 --------DEEFFGFVEVFEDGELLFAIKDDLLRLDKSRKKIKIKTLNPSESTTKATGRY 180
                     E+F  V++FE G ++F +  DL  +D S KK+K+ T  P     K   R+
Sbjct: 278 YDVPKRWWSAEYFQLVKIFEYGTVMFLVDGDLFCIDTSGKKVKMTTFYPD---NKLVTRF 337

Query: 181 T-IKSINFESLSTIL 185
           + I ++NF SL  +L
Sbjct: 338 SNIDALNFGSLPAVL 341

BLAST of CSPI05G29960 vs. NCBI nr
Match: gi|659120418|ref|XP_008460183.1| (PREDICTED: uncharacterized protein LOC103499068 [Cucumis melo])

HSP 1 Score: 105.5 bits (262), Expect = 1.0e-19
Identity = 65/173 (37.57%), Postives = 96/173 (55.49%), Query Frame = 1

Query: 1   MDIFTF------DNGHKQWRHFKRLPFVVIHHGQYLNGIIYWIGKKLEKEGEVVIYALHV 60
           MD+ TF      ++ H QWR    LP + IH+G YLNG+IYWIG K +KE E +IYA++V
Sbjct: 190 MDVLTFTRRSETNHNHSQWRRLHSLP-LRIHNGAYLNGVIYWIGTKEDKENEYIIYAVNV 249

Query: 61  DTEQMECIATLKIG--AFLKNGSIKIYEDRVYAVINLLDKRVSPPCY--KIELWRMQKKD 120
           +TE +E    L +G  +   + S+K +   VYA        ++P  Y   I++WRMQ+KD
Sbjct: 250 ETELIELSFILDLGPCSTSSHASLKQFNSSVYATF-----FINPKTYNSSIQVWRMQEKD 309

Query: 121 SWIKEF-----SADEEFFGFVEVFEDGELLFAIKDDLLRLDKSRKKIKIKTLN 159
            WI+EF       +      ++ FEDGE+L+  K       K R+  +I+ LN
Sbjct: 310 LWIREFVIVDIPINWSSLTLIKAFEDGEILWRKKIVTKNQKKDRRVCQIEYLN 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KUJ1_CUCSA2.2e-2642.08Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643280 PE=4 SV=1[more]
A0A0A0KSN0_CUCSA3.5e-1997.78Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643960 PE=4 SV=1[more]
A0A0A0KSJ2_CUCSA1.7e-1836.82Uncharacterized protein OS=Cucumis sativus GN=Csa_5G643290 PE=4 SV=1[more]
A0A0A0LWG1_CUCSA8.5e-1835.78Uncharacterized protein OS=Cucumis sativus GN=Csa_1G257290 PE=4 SV=1[more]
A0A0A0LPC6_CUCSA6.1e-1635.50Uncharacterized protein OS=Cucumis sativus GN=Csa_1G014420 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|659119533|ref|XP_008459707.1|1.1e-7779.35PREDICTED: putative F-box protein At1g55070 [Cucumis melo][more]
gi|449447691|ref|XP_004141601.1|3.2e-2642.08PREDICTED: F-box protein At3g07870-like [Cucumis sativus][more]
gi|659119531|ref|XP_008459706.1|2.1e-2539.89PREDICTED: uncharacterized protein LOC103498748 [Cucumis melo][more]
gi|659117583|ref|XP_008458678.1|1.2e-2035.90PREDICTED: uncharacterized protein LOC103498006 [Cucumis melo][more]
gi|659120418|ref|XP_008460183.1|1.0e-1937.57PREDICTED: uncharacterized protein LOC103499068 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR017451F-box-assoc_interact_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G29960.1CSPI05G29960.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017451F-box associated interaction domainTIGRFAMsTIGR01640TIGR01640coord: 2..137
score: 3.7