CSPI05G20980 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI05G20980
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	tRNA pseudouridine synthase
Location	Chr5 : 21899160 .. 21903935 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATGATCGAGTACGACCCAGGAAAGCAAACTACCATCGTCTACCTTCGGGCAGTGGCGAAGCAACTTCTTCAACATATGCCGGAGTTCCATAGCACTCATCTTTAACCTCTACAATGCTTTAAACTCACACTAGAACACTTGTTACGCTTCAATCCATGACGGAAAACCAGAAACATGGGCTAGAATTAAGTATAGACTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACCACAGACGAACTTTTTCACGGCGACGAGGAGGAAGCAGAAACCATGAACCCTAAACCTGGAATCCAGCGGTACCTTGTCGCAGTCGAATATATTGGAACTCGGTTTTATGGGTCTCAGCAACAGCCCAACTGCCGTACGGTGGTTGGCGCGCTCGAGGTACTTTCCCATTTTTCTTTCAACTGCCTTTTTCTTATTAACCAATGGAAAACTTTGAAATGGGACATGGCTTATTTAAACCGGCGCTCTGTGTAGCTGTTTAGATGTTTGACCATAAGCACTCTAGAGAGACCAAAAGGAAAGATGAACTTTTAGAGACATAGTTAATGCTTATTTGAGTTTGGGTTTGAGTTGCATACTATCTAGTCACAATGCCAAAGAGGAAGGGTAAAAAATGCAACTAAAAGTGCATAATGGAGGATTTGAAATATAACGTTACACTTAATCGAAGTAATAATGGGAGATCAAAACATGGGTGGTTTATATTAGCCCATATCATCCACCTGTAGTTGGGGTGAGACATCCATTCAAATTCAACGGTTCCCTTCTCTCTCTCTTTGTTGTCTAGTTATCATCGATAATGAATATGAAACATCCGAATTGATATTATCTCATTGCCACTCTGTATCGCTTCCAAGTCACGATAGTTTCAAATGTCATGGAGTTAAGCTAAAGTTTATTTCTATCTATGTTTCTGTTGTCATTTAAATGATTTGAAAAGTAAGATTCTTTTTTTAACAATGCATCTCCTTAATTACACCATGTAGTAGTTGTAAGGAACCGATAGTTGTATTAGCGAGGGCATTAGAGTAAGTAGATAGGGAGCTAGTTAGGGTGTTTGAGGGAGAGGGATCGAGGGAAGACGTGCAATTGTTAAGTGATTAAGGACTTGGGTTAGGATACTCAAGAGGGGCGGTCACAGGGGCCTTATACTTGGTTTATCTTGTAGTTTTCCATACTTTGTATTTCAATATATTTCAAATCTTAATTCCTATTAGGTGGTACCCTAACATACCTCTCTTTCAAAATGATTAACTTAAACTTGTCAGGAAGCATTCCAGAAGTTTATCGGTCAACCAGTCTCAGTGTTTTGTTCTAGCAGAACTGTAAGTTTATCTTATTTTGGATTTTTTTTGTCATACAAAGCAATCTTATTACTACTATTACTTATTAGAATGTACAGTATGCGTGTAGGATATTATTACTTATTATACTAGATGAAGATATAGCCGAATTTAAGTTGAAAAGTAATTTGAAGGCCGTTCTCTCTTTCAAGGTTCCACAACTTTTTTCTTGTTGATTCTTCAGTCGTCTGGCAGTTTTTCTCCCACTAGATTTGATACGCGTAGAGAAGGATATGCAATTGCACTAATTATTTGGCTGTTCACTTATCGATGTTGAAATCCTTGAACTTATATTCATTTGAAATAATGGACTCACATTTATTGTTCTAAGTTGTATTTTGTAACAAATGAACTGTGGCGTTTTAGGATAAGTTACTTTCCTGGCTTGTTTGTTGCAGAGTGCAATTCATCTTGCTCCATATTCGTACATTCTGATATGTGGCAAATTTGAAGGGGATCCATGATGGAGCTGCTTTTTTTATTACATTGATTGTTAGCCAAAGAAAATTAATATTTTGATCTACTTGAAATTTATGGACCAATCATATTCTTATAAATTATGTTTAGTTTAGAACAAATATGCTAATCAGTTTCAGGGTAAGTTATTTTCTTTCTTATAAGAAAAAAGTTTCAGGATAAGTAACTTTGGGATTTTCTGCTGAACTGTAACCTTGCATCATATGCATTTACTACTGTAGTTGTGAAGAACAAATATTTGTCTTAATTTTATCAATGCAATTGTTTTTGTTTTCTTTGTTATTTGAATTCTCATGCGTGTAGTATTTTGTTTCTTTCTAAAATAATTGTATTAGGATATCCGATTCACGTTGTGACTTTCCTTTATTTTTTCTTGGTACGATCAAGGATGCTGGAGTACATGCCTTATCAAATGTTTGTCATGTTGATGTTGAGCGCATAAGCAAAAGAAAGCCTGGAGAAGTGGTCTGTCCCTTTATAAAAGAATATCAGCATCCCTATTTCCTCTCATTTGTACACTTGTAATGTTTGCTCAACTAGATGAGTAATTTTCCCTGAAAACGTTGGTTCACTAGTTGACTTTATTGTTCATTGGCTGTTGGTATCATATTCCATTGCCTTGTGGAGCAGCGTTCCTGCCTTTACTTCTTGGGATTGGCCATAAGACCTAAATTACTTATTTCTAATTCAACTAATTGACTTTATTGTTGAGATTGAAGTTTAAACTTCCTCATGCATATGACTTTGAAGAAATGGAGCTCACTTTGCCACAGAAACGCTAACATCTTTTTCCTAACTATGATGCAGTTACCACCTCATGAACCGGGAGTGGTTCATAAGGCCGTGAATCATTTCTTACAGGTTTGGCTCTTCGCTGACCAATCACTTTCTCCAGCTACTTCTGCTTCTCCTAACTTTCATTGTTTTGATTTTGTGCTTCGTTTTTAAATGGTGCTACATGCAGTTGTCTTGTTATAATGGCCATCTTCAATTGATAATTTTATTCTGTTTGAATTTGCCAGCACGTTAGAATAAACAATCTTCCCTCTTTACTGTTACTAATGAACTTAGATTTAGAACTGCTATATATCCTCTTACCATTGTTATTGACTATTGTTTAAAGGCTATAATATGAGATAGCATCTGAGACATAACTTCGTTTATCCAGCTTGAGCCTTTTGGTTCTTTATTGCTTAACAAAGAAAATGATGAATGAGAGGTATTTATGTTGGTTTTGATTTCAGAAACATGAAGGTGATGTTACTGTAGTTGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACGTGAGTACAATTCATCCATGCTTTAGTAGCTGTCTAGTTGATACCGTTGTTGTATATTAGATAGTAGGATTAATTGTAATCCTTCGTAAGTTGGCCTAATGGTAAGAAGGAGATATAGTCTCAATAAATGGCTAAGAGGTCAAGGTTTCTATTCATGATGGACATCTGCCTACGAGTTTTCTTAACACCCAAATGTAGTAAGGTCAGTCGAGTTGTCCTATGAGATTAGTCGAGGTGCGCATAAGCTGGCCTGGACACTCACAAATATACAAAAAGAAAATAGAAGTATAATTAAATATACATACCTCTATAGAACTTGTTTTGTATCTCATGAAATATTTTCAGGTACTTTTACCGGTTAGTTTCTGGACCAGAAGGTCTCTCAAGCTTTGAGAAGGATCGAGCATGGCACGTGCCTGAGACACTAAATATTGGGGCAATGCAGGTATGAACATAACAATTCATCGCTGGTGATCATGTAGATTTTGAATTACATTATAGAACTGATGCAACTTTATTGGAATGTGTAACGACCTTATTTGAACATGATTTGTTCTATAAGTTGTATGAGTAACTTTTTGAGTTCTAAAAATTATTGGAACATGTGAATTCAGGAGGCATGCAAAGTGCTGGTCGGACATCATGATTTTAGCTCCTTCAGGGCAGCTGGTTGTCAGGTCACATAATACTAAAATTATTTATGTTCCCAGCTTATCATACATTTTGTTTGTAACAAAAGGTGGGGAGTGGTCGGGAAATGAAACGGTGTATGGTGTAGTTTAGATGTGCATTGTGCAGTGCAGGCAAACTCACCAATCAGAACTCTGGATGAACTAAACGTTTGTGAGGTGGCTTCAACTCCTTATTTTCCAACATCAATGGAGCGAAAGCAAAGGCAGGGCAAAGAAGAAAGTGAGATTGAAGTGCCATGTTCAGAAACTTGTGAGGTTGGATTTGGAATCAGGAGAAGGCATCGTTGTTTCGTGGTGACGGCACGAGCACGCTCTTTTCTCTATCATCAGGTTCAAAACCCCAACACTAGACCTGGTGCATGTATGAGAGTGATTTTGAAGTTGATGTTTTATTCAAAATCACTTTGAAACACATTTTTATTCACTTTCAATTAATTTAATATTTAGTTTTATGTTTTTAAATGTGATTTCCATATCATCAAATCTAGTTTTTGGATCATTAAAAGCATTTTAGAATCACTCTCAAACATGTCATTTCTTTATTGTTCATGAGATTGATCAATCAGTTAGTTATTCTCTTCTTAGGTGAGACTGATGGTTGGAGTTCTTAAAGCAGTTGGCTCTGGAGATCTAACCGTAGGAGATGGTAGGTGGCTAGGCAGCAACATATGCAATAGAAATACTTGAAAGTAGTTTTTATATTTGAATTAATAGAACATGGATTGTTCAGTGTAGAACAATAATATGACGTACGTTTGTTATCCACTGCAGTAGGAAGAATATTGGAAGCGAAGAATGTGAGTTCAGCAAGGCCAATGGCTCCTGCATGCGGACTCTACCTCGGCCATGTCAAATACGATCTACCGCCAGCTCCCCATTGAATTCAATCTTGTTGCACCTAATTCCATGTTTTCCTTTTTGTTA

mRNA sequence

ATGACGGAAAACCAGAAACATGGGCTAGAATTAAGTATAGACTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACCACAGACGAACTTTTTCACGGCGACGAGGAGGAAGCAGAAACCATGAACCCTAAACCTGGAATCCAGCGGTACCTTGTCGCAGTCGAATATATTGGAACTCGGTTTTATGGGTCTCAGCAACAGCCCAACTGCCGTACGGTGGTTGGCGCGCTCGAGGAAGCATTCCAGAAGTTTATCGGTCAACCAGTCTCAGTGTTTTGTTCTAGCAGAACTGATGCTGGAGTACATGCCTTATCAAATGTTTGTCATGTTGATGTTGAGCGCATAAGCAAAAGAAAGCCTGGAGAAGTGTTACCACCTCATGAACCGGGAGTGGTTCATAAGGCCGTGAATCATTTCTTACAGAAACATGAAGGTGATGTTACTGTAGTTGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACGTACTTTTACCGGTTAGTTTCTGGACCAGAAGGTCTCTCAAGCTTTGAGAAGGATCGAGCATGGCACGTGCCTGAGACACTAAATATTGGGGCAATGCAGGAGGCATGCAAAGTGCTGGTCGGACATCATGATTTTAGCTCCTTCAGGGCAGCTGGTTGTCAGGCAAACTCACCAATCAGAACTCTGGATGAACTAAACGTTTGTGAGGTGGCTTCAACTCCTTATTTTCCAACATCAATGGAGCGAAAGCAAAGGCAGGGCAAAGAAGAAAGTGAGATTGAAGTGCCATGTTCAGAAACTTGTGAGGTTGGATTTGGAATCAGGAGAAGGCATCGTTGTTTCGTGGTGACGGCACGAGCACGCTCTTTTCTCTATCATCAGGTGAGACTGATGGTTGGAGTTCTTAAAGCAGTTGGCTCTGGAGATCTAACCGTAGGAGATGTAGGAAGAATATTGGAAGCGAAGAATGTGAGTTCAGCAAGGCCAATGGCTCCTGCATGCGGACTCTACCTCGGCCATGTCAAATACGATCTACCGCCAGCTCCCCATTGA

Coding sequence (CDS)

ATGACGGAAAACCAGAAACATGGGCTAGAATTAAGTATAGACTCATCATCTCAAAGGCCCTTGAAAATTGGAAAGACCACAGACGAACTTTTTCACGGCGACGAGGAGGAAGCAGAAACCATGAACCCTAAACCTGGAATCCAGCGGTACCTTGTCGCAGTCGAATATATTGGAACTCGGTTTTATGGGTCTCAGCAACAGCCCAACTGCCGTACGGTGGTTGGCGCGCTCGAGGAAGCATTCCAGAAGTTTATCGGTCAACCAGTCTCAGTGTTTTGTTCTAGCAGAACTGATGCTGGAGTACATGCCTTATCAAATGTTTGTCATGTTGATGTTGAGCGCATAAGCAAAAGAAAGCCTGGAGAAGTGTTACCACCTCATGAACCGGGAGTGGTTCATAAGGCCGTGAATCATTTCTTACAGAAACATGAAGGTGATGTTACTGTAGTTGATGTTCGAAGTGTTCCCAATGATTTTCATGCTAGATATAAAGCTCAAGAGCGTACGTACTTTTACCGGTTAGTTTCTGGACCAGAAGGTCTCTCAAGCTTTGAGAAGGATCGAGCATGGCACGTGCCTGAGACACTAAATATTGGGGCAATGCAGGAGGCATGCAAAGTGCTGGTCGGACATCATGATTTTAGCTCCTTCAGGGCAGCTGGTTGTCAGGCAAACTCACCAATCAGAACTCTGGATGAACTAAACGTTTGTGAGGTGGCTTCAACTCCTTATTTTCCAACATCAATGGAGCGAAAGCAAAGGCAGGGCAAAGAAGAAAGTGAGATTGAAGTGCCATGTTCAGAAACTTGTGAGGTTGGATTTGGAATCAGGAGAAGGCATCGTTGTTTCGTGGTGACGGCACGAGCACGCTCTTTTCTCTATCATCAGGTGAGACTGATGGTTGGAGTTCTTAAAGCAGTTGGCTCTGGAGATCTAACCGTAGGAGATGTAGGAAGAATATTGGAAGCGAAGAATGTGAGTTCAGCAAGGCCAATGGCTCCTGCATGCGGACTCTACCTCGGCCATGTCAAATACGATCTACCGCCAGCTCCCCATTGA

Homology

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BLAST of CSPI05G20980 vs. Swiss-Prot
Match: TRUA_PARL1 (tRNA pseudouridine synthase A OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) GN=truA PE=3 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 9.0e-46
Identity = 112/298 (37.58%), Postives = 156/298 (52.35%), Query Frame = 1


Query: 49  RYLVAVEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVC 108
           RY + +EY G+ F G Q Q N R+V   LEE  + F GQ + +F + RTDAGVHAL  V 
Sbjct: 3   RYKLTIEYDGSPFVGWQAQTNGRSVQQVLEEGIKGFCGQELKIFGAGRTDAGVHALGQVA 62

Query: 109 HVDVERISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQER 168
           HVD E            P  P  +  AVN  ++ H   V +V+   VP+ F AR+ A +R
Sbjct: 63  HVDFET-----------PVAPDTLRDAVNAHMRPHP--VAIVEAEEVPDSFEARFSAVKR 122

Query: 169 TYFYRLVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPI 228
            Y YR+V+    L+  ++ +AW V +TL+  AM +A + LVG+HDF++FR+  CQA SP+
Sbjct: 123 HYMYRIVNRRAPLT-LDRGQAWLVHKTLDADAMHDAAQALVGNHDFTTFRSVQCQAKSPV 182

Query: 229 RTLDELNVCEVASTPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTAR 288
           +T+DE++V   A                    EIE+ C                      
Sbjct: 183 KTVDEISVSRYA-------------------DEIEIVCR--------------------- 242

Query: 289 ARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYD 347
           ARSFL++QVR  VG LK VG G  T   V + L AK+ ++  P+AP  GLYL  V Y+
Sbjct: 243 ARSFLHNQVRSFVGTLKMVGDGSWTRRKVEKALAAKDRAACGPVAPPDGLYLLQVDYE 246

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BLAST of CSPI05G20980 vs. Swiss-Prot
Match: TRUA_RHOCS (tRNA pseudouridine synthase A OS=Rhodospirillum centenum (strain ATCC 51521 / SW) GN=truA PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 5.0e-44
Identity = 120/299 (40.13%), Postives = 152/299 (50.84%), Query Frame = 1


Query: 47  IQRYLVAVEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSN 106
           +QR+ + +EY G  F G Q+Q N  +V  ALEEA  +F  + V+VFC+ RTDAGVHAL+ 
Sbjct: 1   MQRWKLTLEYDGRPFVGWQRQDNGPSVQQALEEAIYRFTQETVTVFCAGRTDAGVHALAM 60

Query: 107 VCHVDVERISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQ 166
           V  VD+ R +               V  A+N  L+ H   V V+ V  V  DFHAR+   
Sbjct: 61  VASVDLAREATADK-----------VQAALNFHLKPHP--VAVLRVEPVAPDFHARFSCL 120

Query: 167 ERTYFYRLVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANS 226
            R Y YR+++     ++ E  R WHV   L+  AM EA + LVG HDFSSFRA+ CQA S
Sbjct: 121 GRAYLYRILNR-RAPAALEAGRVWHVQGGLDAEAMHEAAQRLVGQHDFSSFRASLCQAKS 180

Query: 227 PIRTLDELNVCEVASTPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVT 286
           P                             K  S++ V       VG  +R       + 
Sbjct: 181 P----------------------------VKTLSDLSV-----ARVGEEVR-------IV 240

Query: 287 ARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKY 346
           ARARSFL+HQVR MVG LK VG G  T  DV R L A+N S+A P APA GLY     Y
Sbjct: 241 ARARSFLHHQVRNMVGTLKLVGEGRWTADDVSRALAARNRSAAGPTAPAAGLYFTEAWY 245

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BLAST of CSPI05G20980 vs. Swiss-Prot
Match: TRUA_ROSDO (tRNA pseudouridine synthase A OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) GN=truA PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.1e-43
Identity = 107/303 (35.31%), Postives = 162/303 (53.47%), Query Frame = 1


Query: 47  IQRYLVAVEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSN 106
           + RY + VEY G  F G Q+Q    T+ GA+E A  K    P ++  + RTDAGVHA++ 
Sbjct: 1   MHRYALKVEYNGAPFVGWQRQKEHPTIQGAIERALGKIAPGPHTIAAAGRTDAGVHAIAQ 60

Query: 107 VCHVDVERISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQ 166
           V H D+ER             +P  + +A+N+ L+     + +    +V  ++HAR+ A+
Sbjct: 61  VAHCDLER-----------DWDPFRLSEALNYHLKP--APIAITACTAVDAEWHARFSAR 120

Query: 167 ERTYFYRLVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANS 226
           +R Y +R++      ++ +K   W V + L++ AM+EA  +L+G HDF++FR+  CQA S
Sbjct: 121 QRQYLFRILCR-RAPATHQKGLVWQVKQKLDVTAMREAAALLIGQHDFTTFRSTICQAES 180

Query: 227 PIRTLDELNVCEVASTPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVT 286
           P++TLD L+V EV  TPY           G+E             + F +R         
Sbjct: 181 PVKTLDRLDVNEV-DTPY-----------GRE-------------IHFDVR--------- 240

Query: 287 ARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYD 346
             ARSFL++QVR  VG L+ VG+G  T  DV R L  ++ ++  P+ P  GLYL HV YD
Sbjct: 241 --ARSFLHNQVRSFVGTLERVGAGSSTPQDVARALALRDRAACGPVCPPHGLYLAHVVYD 253

Query: 347 LPP 350
            PP
Sbjct: 301 RPP 253

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BLAST of CSPI05G20980 vs. Swiss-Prot
Match: PUSL1_HUMAN (tRNA pseudouridine synthase-like 1 OS=Homo sapiens GN=PUSL1 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 4.3e-40
Identity = 119/307 (38.76%), Postives = 160/307 (52.12%), Query Frame = 1


Query: 49  RYLVAVEYIGTRFYGSQQQPNCRTVVGA---LEEAFQKFIG-QPVSVFCSSRTDAGVHAL 108
           RYLV  +Y+GT F G       +  VG    LEEA ++    +PV    SSRTDAGVHAL
Sbjct: 13  RYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVRFTISSRTDAGVHAL 72

Query: 109 SNVCHVDVERISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYK 168
           SN  H+DV+R S R      PP  P V+ +A+N  L +H   + V+    VP+DFHAR+ 
Sbjct: 73  SNAAHLDVQRRSGR------PPFPPEVLAEALNTHL-RHPA-IRVLRAFRVPSDFHARHA 132

Query: 169 AQERTYFYRLVSG---PEGLSSFEKDRAWHVP-ETLNIGAMQEACKVLVGHHDFSSFRAA 228
           A  RTY YRL +G    + L  FE++  W +P + L++ AMQEA + L+G HDFS+F++A
Sbjct: 133 ATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAFQSA 192

Query: 229 GCQANSPIRTLDELNVCEVASTPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRH 288
           G    SP+RTL  ++V    ++P       RK R    E E                   
Sbjct: 193 GSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFE------------------- 252

Query: 289 RCFVVTARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARP--MAPACGL 346
                   ++SFLY QVR M  VL AVG G L    V  ILE+++        +APA GL
Sbjct: 253 --------SQSFLYRQVRRMTAVLVAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGL 284

[Close]

BLAST of CSPI05G20980 vs. Swiss-Prot
Match: PUSL1_MOUSE (tRNA pseudouridine synthase-like 1 OS=Mus musculus GN=Pusl1 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.7e-39
Identity = 119/309 (38.51%), Postives = 163/309 (52.75%), Query Frame = 1


Query: 49  RYLVAVEYIGTRFYGS---QQQPNCRTVVGALEEAFQKFIG-QPVSVFCSSRTDAGVHAL 108
           RYLV ++Y+GT F G    +  P    V+  LEEA ++     PV    SSRTDAGVHAL
Sbjct: 13  RYLVFLQYLGTDFNGVAAVRGNPRAVGVLNFLEEAAKRLNSVDPVRFTISSRTDAGVHAL 72

Query: 109 SNVCHVDVERISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYK 168
           SN  H+D++R    +PG+   P  P VV KA+N  L KH   + V+    VPNDFHAR+ 
Sbjct: 73  SNAAHLDIQR----RPGQ--SPFSPEVVAKALNTHL-KHPA-IRVLKAFRVPNDFHARHA 132

Query: 169 AQERTYFYRLVSG---PEGLSSFEKDRAWHVP-ETLNIGAMQEACKVLVGHHDFSSFRAA 228
           A  RTY YRL +G   P  L  FE++  W +  E L++ AMQEA + L+G HDFS+F++A
Sbjct: 133 ATSRTYQYRLATGCSWPNQLPVFEQNVCWALQTEYLDMAAMQEAAQHLLGTHDFSAFQSA 192

Query: 229 GCQANSPIRTLDELNVCEVASTPY-FPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRR 288
           G    + +RTL  ++V    ++P+  P                               RR
Sbjct: 193 GSPVTNTVRTLRRVSVSPGPASPFVLPEG----------------------------SRR 252

Query: 289 HRCFVVTARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNV--SSARPMAPACG 347
            + + +   ++SFLY QVR M  VL AVG G L    V  ILE+++        +APA G
Sbjct: 253 LQFWTLEFESQSFLYRQVRRMTAVLVAVGLGILAPTQVKVILESQDPLGKYQARVAPARG 285

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BLAST of CSPI05G20980 vs. TrEMBL
Match: A0A0A0KRX0_CUCSA (tRNA pseudouridine synthase OS=Cucumis sativus GN=Csa_5G586020 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 4.6e-206
Identity = 351/352 (99.72%), Postives = 351/352 (99.72%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60
           MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120
           FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEG 180
           GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240
           LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 353
           VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH
Sbjct: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 352

[Close]

BLAST of CSPI05G20980 vs. TrEMBL
Match: A0A061DW68_THECC (tRNA pseudouridine synthase OS=Theobroma cacao GN=TCM_006169 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 8.0e-142
Identity = 262/376 (69.68%), Postives = 291/376 (77.39%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAE--------TMNPKPGIQRYLV 60
           M+EN KH L  + +SS  RP KI KT D   H D+EE E        +M+  P IQRYLV
Sbjct: 1   MSENPKHSLSTTPESS--RPSKIPKTQD---HSDQEEEEEDKQQQESSMSKNPRIQRYLV 60

Query: 61  AVEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDV 120
           A+EYIGTRF GSQQQPNCRTVVG LEEAF KFIGQPVS+FCSSRTDAGVHALSNVCHVDV
Sbjct: 61  AIEYIGTRFSGSQQQPNCRTVVGVLEEAFLKFIGQPVSIFCSSRTDAGVHALSNVCHVDV 120

Query: 121 ERISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFY 180
           ERISKRKPGEVLPPHEP VV +AVNHFLQK EGD+TV+DVR VPNDFHARYKAQERTYFY
Sbjct: 121 ERISKRKPGEVLPPHEPSVVRRAVNHFLQKSEGDITVIDVRCVPNDFHARYKAQERTYFY 180

Query: 181 RLVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLD 240
           RL+SG E LS+FEK+RAWHVPE L++ AMQEACKVLVGHHDFSSFRAAGCQA SPIRTLD
Sbjct: 181 RLLSGSEPLSTFEKERAWHVPEKLDLYAMQEACKVLVGHHDFSSFRAAGCQAKSPIRTLD 240

Query: 241 ELNVCEVASTPYFPTSMERKQRQGKEE--------------------SEIEVPCSETCEV 300
           EL+V E   TPYFP+  E+KQ     E                    S+ EV  +   ++
Sbjct: 241 ELHVSEAPPTPYFPSISEKKQNNSSTEDSHACSREPEIDLPIDSFSNSDDEVGSNGITDL 300

Query: 301 GFGIRRRHRCFVVTARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARPM 349
           GFGIRRRH C+VVTARARSFLYHQVRL+VGVLK VG+G+LT  DV RIL AK V++A PM
Sbjct: 301 GFGIRRRHHCYVVTARARSFLYHQVRLLVGVLKCVGTGELTTSDVERILNAKTVTAASPM 360

[Close]

BLAST of CSPI05G20980 vs. TrEMBL
Match: K4B1T1_SOLLC (tRNA pseudouridine synthase OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 4.4e-140
Identity = 253/377 (67.11%), Postives = 296/377 (78.51%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDE----LFHGDEEE---AETMNPKPGIQRYLVA 60
           ++E  KH L+   +S  QRP K+ K  D+    +  G+E++     TMNP P +QRYL+A
Sbjct: 10  ISETNKHSLDQRPESP-QRPSKLPKVNDDGGNDVVEGEEQKESVTRTMNPNPRLQRYLLA 69

Query: 61  VEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVE 120
           +EYIGTRF G+QQQPNCRTVVG L+EAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVE
Sbjct: 70  IEYIGTRFAGAQQQPNCRTVVGVLQEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVE 129

Query: 121 RISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYR 180
           RISKRKPG+VLPPHEP VV +AVNHFLQK+ GD+ V+DVR VP+DFHARYKAQERTYFYR
Sbjct: 130 RISKRKPGDVLPPHEPLVVKRAVNHFLQKNAGDIMVIDVRCVPDDFHARYKAQERTYFYR 189

Query: 181 LVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDE 240
           L+SGPE +SSFE+DRAWHVPE L++ AMQ+AC +L+G HDFSSFRAA CQANSPIRTLDE
Sbjct: 190 LLSGPEPVSSFERDRAWHVPENLDLLAMQKACNILIGRHDFSSFRAAACQANSPIRTLDE 249

Query: 241 LNVCEVASTPYFPTSMERKQRQ---------------------GKEESEIEVPCSETCEV 300
           LNV EV STP+FP+  ER +                          E ++E    E C+ 
Sbjct: 250 LNVIEVVSTPFFPSISERLKSSSVMEDPTVYLGSDTNHSQISFNTNEGKLEGSNGEACQ- 309

Query: 301 GFGIRRRHRCFVVTARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARPM 350
            FG+RRRHRCFVVTARARSFLYHQVRL+VGVLK+VG+GDLT  DV RILEAK++++A PM
Sbjct: 310 EFGLRRRHRCFVVTARARSFLYHQVRLLVGVLKSVGAGDLTDPDVKRILEAKSIAAAPPM 369

[Close]

BLAST of CSPI05G20980 vs. TrEMBL
Match: A0A061DY96_THECC (tRNA pseudouridine synthase OS=Theobroma cacao GN=TCM_006169 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 4.4e-140
Identity = 261/376 (69.41%), Postives = 290/376 (77.13%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAE--------TMNPKPGIQRYLV 60
           M+EN KH L  + +SS  RP KI KT D   H D+EE E        +M+  P IQRYLV
Sbjct: 1   MSENPKHSLSTTPESS--RPSKIPKTQD---HSDQEEEEEDKQQQESSMSKNPRIQRYLV 60

Query: 61  AVEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDV 120
           A+EYIGTRF GSQQQPNCRTVVG LEEAF KFIGQPVS+FCSSRTDAGVHALSNVCHVDV
Sbjct: 61  AIEYIGTRFSGSQQQPNCRTVVGVLEEAFLKFIGQPVSIFCSSRTDAGVHALSNVCHVDV 120

Query: 121 ERISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFY 180
           ERISKRKPGEVLPPHEP VV +AVNHFLQ  EGD+TV+DVR VPNDFHARYKAQERTYFY
Sbjct: 121 ERISKRKPGEVLPPHEPSVVRRAVNHFLQS-EGDITVIDVRCVPNDFHARYKAQERTYFY 180

Query: 181 RLVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLD 240
           RL+SG E LS+FEK+RAWHVPE L++ AMQEACKVLVGHHDFSSFRAAGCQA SPIRTLD
Sbjct: 181 RLLSGSEPLSTFEKERAWHVPEKLDLYAMQEACKVLVGHHDFSSFRAAGCQAKSPIRTLD 240

Query: 241 ELNVCEVASTPYFPTSMERKQRQGKEE--------------------SEIEVPCSETCEV 300
           EL+V E   TPYFP+  E+KQ     E                    S+ EV  +   ++
Sbjct: 241 ELHVSEAPPTPYFPSISEKKQNNSSTEDSHACSREPEIDLPIDSFSNSDDEVGSNGITDL 300

Query: 301 GFGIRRRHRCFVVTARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARPM 349
           GFGIRRRH C+VVTARARSFLYHQVRL+VGVLK VG+G+LT  DV RIL AK V++A PM
Sbjct: 301 GFGIRRRHHCYVVTARARSFLYHQVRLLVGVLKCVGTGELTTSDVERILNAKTVTAASPM 360

[Close]

BLAST of CSPI05G20980 vs. TrEMBL
Match: M1CBM9_SOLTU (tRNA pseudouridine synthase OS=Solanum tuberosum GN=PGSC0003DMG400024892 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.3e-139
Identity = 252/377 (66.84%), Postives = 294/377 (77.98%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDE----LFHGDEEE---AETMNPKPGIQRYLVA 60
           ++E  KH L+   +S  QRP K  K  D+    +  G+E++     TMNP P +QRYL+A
Sbjct: 10  ISETNKHSLDQRPESP-QRPSKFPKVNDDAGNDVLEGEEQKESVTRTMNPNPRLQRYLIA 69

Query: 61  VEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVE 120
           +EYIGTRF G+QQQPNCRTVVG L+EAFQKF+GQPVSVFCSSRTDAGVHALSNVCHVDVE
Sbjct: 70  IEYIGTRFAGAQQQPNCRTVVGVLQEAFQKFVGQPVSVFCSSRTDAGVHALSNVCHVDVE 129

Query: 121 RISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYR 180
           RISKRKPG+VLPPHEP VV +AVNHFLQK+EGD+ V+DVR VP DFHARYKAQERTYFYR
Sbjct: 130 RISKRKPGDVLPPHEPLVVKRAVNHFLQKNEGDIMVIDVRCVPADFHARYKAQERTYFYR 189

Query: 181 LVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDE 240
           L+SGPE +SSFE+DRAWHVPE L++ AMQ+AC +L+G HDFSSFRAA CQANSPIRTLDE
Sbjct: 190 LLSGPESVSSFERDRAWHVPENLDLLAMQKACNILIGRHDFSSFRAAACQANSPIRTLDE 249

Query: 241 LNVCEVASTPYFPTSMERKQRQG---------------------KEESEIEVPCSETCEV 300
           LNV EV STP+FP+  ER +                          E ++E    E C+ 
Sbjct: 250 LNVIEVVSTPFFPSISERLKSSSVMEDPTVSLGSDTNHSQISLNTNEEKLEGSNGEACQE 309

Query: 301 GFGIRRRHRCFVVTARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARPM 350
            FG+RRRHRCFVVTARARSFLYHQVRL+VGVLK+VG+G LT  DV RILEAK++++A PM
Sbjct: 310 -FGLRRRHRCFVVTARARSFLYHQVRLLVGVLKSVGAGVLTDPDVKRILEAKSITAAPPM 369

[Close]

BLAST of CSPI05G20980 vs. TAIR10
Match: AT1G09800.1 (AT1G09800.1 Pseudouridine synthase family protein)

HSP 1 Score: 459.5 bits (1181), Expect = 1.8e-129
Identity = 239/374 (63.90%), Postives = 277/374 (74.06%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSS-QRPLKIGKTTDELFHGDEEEA-------ETMNP-KPGIQRYL 60
           M++N KH L+ +    S +R  K+ +   E F  +E E        E+MNP  P +QRYL
Sbjct: 1   MSDNLKHPLDCTASEESLERLSKVARI--EGFSDEESEEQQGLADLESMNPVNPRVQRYL 60

Query: 61  VAVEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVD 120
           VA+EYIGTRF GSQQQ   RTVVG L+EAF KFIGQPV +FCSSRTDAGVHALSNVCHVD
Sbjct: 61  VAIEYIGTRFSGSQQQAKDRTVVGVLQEAFHKFIGQPVKIFCSSRTDAGVHALSNVCHVD 120

Query: 121 VERISKRKPGEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYF 180
           VERISKRKPGEVLPPHEPGVV +AVNHFLQ+ +GDV V+DVR VP+++HARYKAQERTYF
Sbjct: 121 VERISKRKPGEVLPPHEPGVVQRAVNHFLQRKDGDVMVIDVRCVPSNYHARYKAQERTYF 180

Query: 181 YRLVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTL 240
           YRL+SG + LS  EKDR WHVPE LN+  MQEAC+VLVG HDFSSFRAAGCQA SP+R+L
Sbjct: 181 YRLLSGSDPLSILEKDRCWHVPEELNLRFMQEACRVLVGSHDFSSFRAAGCQAKSPMRSL 240

Query: 241 DELNVCEVASTPYFPTSMER---KQRQGKEESEIEVPCSETCEV--------------GF 300
           DELN+ EV STPYFP+  ER       G   +    P SET  V               F
Sbjct: 241 DELNITEVPSTPYFPSITERTLSNLNNGDPLACSNQPKSETAGVTTNVGEVEGSTEGDSF 300

Query: 301 GIRRRHRCFVVTARARSFLYHQVRLMVGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAP 349
           G RRRHRC+VVTAR+R FLYHQVRL+VG LK VG+G+LTV DV RILEAK VS+A+PMAP
Sbjct: 301 GRRRRHRCYVVTARSRGFLYHQVRLIVGALKCVGTGELTVSDVERILEAKTVSAAKPMAP 360

[Close]

BLAST of CSPI05G20980 vs. TAIR10
Match: AT3G06950.1 (AT3G06950.1 Pseudouridine synthase family protein)

HSP 1 Score: 87.4 bits (215), Expect = 1.9e-17
Identity = 59/203 (29.06%), Postives = 98/203 (48.28%), Query Frame = 1


Query: 46  GIQRYLVAVEYIGTRFYGSQQQPNCRTVVGALEEAFQKFIG---QPVSVFCSSRTDAGVH 105
           G  ++ + + Y GTRF G Q Q +  T+   LE+A  +      + + +  + RTDAGVH
Sbjct: 39  GAYKWRLVIAYDGTRFAGWQYQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVH 98

Query: 106 ALSNVCHVDVERISKRKPGEVLPPHEPGV--VHKAVNHFLQKHEGDVTVVDVRSVPNDFH 165
           A   V H             V P +   +   H A+N  L K   D+ V ++ +   +FH
Sbjct: 99  AWGQVAHF------------VTPFNYTSLDSFHAALNGLLPK---DIRVRELSAAVPEFH 158

Query: 166 ARYKAQERTYFYRLVSGPEGLSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAA 225
           AR+ A  + Y Y++ +    +  F++  A+H    LN   M+EA  + VG HDFS+F  A
Sbjct: 159 ARFSASSKVYRYQIYNDTF-MDPFQRHWAYHCAYKLNASKMREAANLFVGKHDFSAFANA 218

Query: 226 GCQ--ANSPIRTLDELNVCEVAS 242
             +     P++T+   +V ++ S
Sbjct: 219 TREDGVPDPLKTISRFDVIQMGS 225

[Close]

BLAST of CSPI05G20980 vs. NCBI nr
Match: gi|778704462|ref|XP_011655540.1| (PREDICTED: tRNA pseudouridine synthase-like 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 724.9 bits (1870), Expect = 6.6e-206
Identity = 351/352 (99.72%), Postives = 351/352 (99.72%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60
           MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120
           FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEG 180
           GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240
           LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 353
           VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH
Sbjct: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 352

[Close]

BLAST of CSPI05G20980 vs. NCBI nr
Match: gi|659090353|ref|XP_008445970.1| (PREDICTED: tRNA pseudouridine synthase-like 1 isoform X1 [Cucumis melo])

HSP 1 Score: 705.3 bits (1819), Expect = 5.4e-200
Identity = 343/352 (97.44%), Postives = 346/352 (98.30%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60
           MTENQKHGLELSIDSSSQRPLKIGKTTD++FHGDEEEAETMNPKPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120
           F GSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEG 180
           GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240
           LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEESE E P SETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 353
           VGVLKAVG+GDLTVGDV RILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH
Sbjct: 301 VGVLKAVGTGDLTVGDVERILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 352

[Close]

BLAST of CSPI05G20980 vs. NCBI nr
Match: gi|659090355|ref|XP_008445972.1| (PREDICTED: tRNA pseudouridine synthase-like 1 isoform X2 [Cucumis melo])

HSP 1 Score: 699.1 bits (1803), Expect = 3.9e-198
Identity = 342/352 (97.16%), Postives = 345/352 (98.01%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60
           MTENQKHGLELSIDSSSQRPLKIGKTTD++FHGDEEEAETMNPKPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120
           F GSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120

Query: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEG 180
           GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 
Sbjct: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240
           LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEESE E P SETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 353
           VGVLKAVG+GDLTVGD  RILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH
Sbjct: 301 VGVLKAVGTGDLTVGD-ERILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 351

[Close]

BLAST of CSPI05G20980 vs. NCBI nr
Match: gi|778704466|ref|XP_011655541.1| (PREDICTED: tRNA pseudouridine synthase-like 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 657.1 bits (1694), Expect = 1.7e-185
Identity = 325/352 (92.33%), Postives = 325/352 (92.33%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60
           MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120
           FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRT                       
Sbjct: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRT----------------------- 120

Query: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEG 180
              LPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 
Sbjct: 121 ---LPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240
           LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 353
           VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH
Sbjct: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 326

[Close]

BLAST of CSPI05G20980 vs. NCBI nr
Match: gi|659090357|ref|XP_008445973.1| (PREDICTED: tRNA pseudouridine synthase-like 1 isoform X3 [Cucumis melo])

HSP 1 Score: 637.5 bits (1643), Expect = 1.4e-179
Identity = 317/352 (90.06%), Postives = 320/352 (90.91%), Query Frame = 1


Query: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDELFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60
           MTENQKHGLELSIDSSSQRPLKIGKTTD++FHGDEEEAETMNPKPGIQRYLVAVEYIGTR
Sbjct: 1   MTENQKHGLELSIDSSSQRPLKIGKTTDQVFHGDEEEAETMNPKPGIQRYLVAVEYIGTR 60

Query: 61  FYGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRTDAGVHALSNVCHVDVERISKRKP 120
           F GSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRT                       
Sbjct: 61  FSGSQQQPNCRTVVGALEEAFQKFIGQPVSVFCSSRT----------------------- 120

Query: 121 GEVLPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEG 180
              LPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPE 
Sbjct: 121 ---LPPHEPGVVHKAVNHFLQKHEGDVTVVDVRSVPNDFHARYKAQERTYFYRLVSGPEA 180

Query: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240
           LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA
Sbjct: 181 LSSFEKDRAWHVPETLNIGAMQEACKVLVGHHDFSSFRAAGCQANSPIRTLDELNVCEVA 240

Query: 241 STPYFPTSMERKQRQGKEESEIEVPCSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300
           STPYFPTSMERKQRQGKEESE E P SETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM
Sbjct: 241 STPYFPTSMERKQRQGKEESENEEPHSETCEVGFGIRRRHRCFVVTARARSFLYHQVRLM 300

Query: 301 VGVLKAVGSGDLTVGDVGRILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 353
           VGVLKAVG+GDLTVGDV RILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH
Sbjct: 301 VGVLKAVGTGDLTVGDVERILEAKNVSSARPMAPACGLYLGHVKYDLPPAPH 326

The following BLAST results are available for this feature:

BLAST of CSPI05G20980 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TRUA_PARL1	9.0e-46	37.58	tRNA pseudouridine synthase A OS=Parvibaculum lavamentivorans (strain DS-1 / DSM...	[more]
TRUA_RHOCS	5.0e-44	40.13	tRNA pseudouridine synthase A OS=Rhodospirillum centenum (strain ATCC 51521 / SW...	[more]
TRUA_ROSDO	1.1e-43	35.31	tRNA pseudouridine synthase A OS=Roseobacter denitrificans (strain ATCC 33942 / ...	[more]
PUSL1_HUMAN	4.3e-40	38.76	tRNA pseudouridine synthase-like 1 OS=Homo sapiens GN=PUSL1 PE=1 SV=1	[more]
PUSL1_MOUSE	3.7e-39	38.51	tRNA pseudouridine synthase-like 1 OS=Mus musculus GN=Pusl1 PE=1 SV=1	[more]

BLAST of CSPI05G20980 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KRX0_CUCSA	4.6e-206	99.72	tRNA pseudouridine synthase OS=Cucumis sativus GN=Csa_5G586020 PE=3 SV=1	[more]
A0A061DW68_THECC	8.0e-142	69.68	tRNA pseudouridine synthase OS=Theobroma cacao GN=TCM_006169 PE=3 SV=1	[more]
K4B1T1_SOLLC	4.4e-140	67.11	tRNA pseudouridine synthase OS=Solanum lycopersicum PE=3 SV=1	[more]
A0A061DY96_THECC	4.4e-140	69.41	tRNA pseudouridine synthase OS=Theobroma cacao GN=TCM_006169 PE=3 SV=1	[more]
M1CBM9_SOLTU	1.3e-139	66.84	tRNA pseudouridine synthase OS=Solanum tuberosum GN=PGSC0003DMG400024892 PE=3 SV...	[more]

BLAST of CSPI05G20980 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G09800.1	1.8e-129	63.90	Pseudouridine synthase family protein	[more]
AT3G06950.1	1.9e-17	29.06	Pseudouridine synthase family protein	[more]

BLAST of CSPI05G20980 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778704462\|ref\|XP_011655540.1\|	6.6e-206	99.72	PREDICTED: tRNA pseudouridine synthase-like 1 isoform X1 [Cucumis sativus]	[more]
gi\|659090353\|ref\|XP_008445970.1\|	5.4e-200	97.44	PREDICTED: tRNA pseudouridine synthase-like 1 isoform X1 [Cucumis melo]	[more]
gi\|659090355\|ref\|XP_008445972.1\|	3.9e-198	97.16	PREDICTED: tRNA pseudouridine synthase-like 1 isoform X2 [Cucumis melo]	[more]
gi\|778704466\|ref\|XP_011655541.1\|	1.7e-185	92.33	PREDICTED: tRNA pseudouridine synthase-like 1 isoform X2 [Cucumis sativus]	[more]
gi\|659090357\|ref\|XP_008445973.1\|	1.4e-179	90.06	PREDICTED: tRNA pseudouridine synthase-like 1 isoform X3 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001406	PsdUridine_synth_TruA
IPR020094	Pseudouridine synthase I, TruA, N-terminal
IPR020095	PsdUridine_synth_TruA_C
IPR020097	PsdUridine_synth_TruA_a/b_dom
IPR020103	PsdUridine_synth_cat_dom_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0001522	pseudouridine synthesis
GO:0009451	RNA modification

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0009982	pseudouridine synthase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0008033	tRNA processing
biological_process	GO:0009451	RNA modification
biological_process	GO:0019243	methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G20980.1	CSPI05G20980.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001406	Pseudouridine synthase I, TruA	HAMAP	MF_00171	TruA	coord: 48..345 score: 2
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 1..249 score: 6.3E-208coord: 280..346 score: 6.3E
IPR020094	Pseudouridine synthase I, TruA, N-terminal	GENE3D	G3DSA:3.30.70.580		coord: 48..165 score: 7.0
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	G3DSA:3.30.70.660		coord: 167..238 score: 5.0E-48coord: 279..344 score: 5.0
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 53..163 score: 2.0E-7coord: 204..346 score: 5.4
IPR020103	Pseudouridine synthase, catalytic domain	unknown	SSF55120	Pseudouridine synthase	coord: 282..346 score: 9.21E-71coord: 47..241 score: 9.21
None	No IPR available	PANTHER	PTHR11142:SF1	TRNA PSEUDOURIDINE SYNTHASE	coord: 1..249 score: 6.3E-208coord: 280..346 score: 6.3E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI05G20980	Cla022928	Watermelon (97103) v1	cpiwmB444
CSPI05G20980	Csa5G586020	Cucumber (Chinese Long) v2	cpicuB239
CSPI05G20980	MELO3C011820	Melon (DHL92) v3.5.1	cpimeB369
CSPI05G20980	ClCG11G013270	Watermelon (Charleston Gray)	cpiwcgB369
CSPI05G20980	Lsi04G007180	Bottle gourd (USVL1VR-Ls)	cpilsiB372
CSPI05G20980	MELO3C011820.2	Melon (DHL92) v3.6.1	cpimedB363
CSPI05G20980	CsaV3_5G029790	Cucumber (Chinese Long) v3	cpicucB284
CSPI05G20980	Cla97C11G219190	Watermelon (97103) v2	cpiwmbB373
CSPI05G20980	BhiUN887G1	Wax gourd	cpiwgoB463
CSPI05G20980	Cucsa.076140	Cucumber (Gy14) v1	cgycpiB076
CSPI05G20980	CmaCh04G018630	Cucurbita maxima (Rimu)	cmacpiB758
CSPI05G20980	CmoCh04G019610	Cucurbita moschata (Rifu)	cmocpiB748
CSPI05G20980	Cp4.1LG01g16420	Cucurbita pepo (Zucchini)	cpecpiB440
CSPI05G20980	CsGy5G020500	Cucumber (Gy14) v2	cgybcpiB225
CSPI05G20980	Carg25942	Silver-seed gourd	carcpiB0388
CSPI05G20980	Carg24425	Silver-seed gourd	carcpiB0153

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI05G20980	Melon (DHL92) v3.6.1	cpimedB360
CSPI05G20980	Silver-seed gourd	carcpiB0727
CSPI05G20980	Cucumber (Chinese Long) v3	cpicucB297
CSPI05G20980	Cucurbita maxima (Rimu)	cmacpiB419
CSPI05G20980	Cucurbita moschata (Rifu)	cmocpiB412
CSPI05G20980	Melon (DHL92) v3.5.1	cpimeB368
CSPI05G20980	Cucurbita pepo (Zucchini)	cpecpiB042