CSPI05G02150 (gene) Wild cucumber (PI 183967)

NameCSPI05G02150
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionDefensin
LocationChr5 : 2887731 .. 2888291 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAACCCTCTTGTACATGGCTTTAAATGTATTTTATTCCCATATTTATCCTTAAATTAGGGTTATCCTCTCCTTTAACATATATAATAAACAATTTAATTCGGTATTATAGTGAATTAAACTCATGACCCCTCTTTTTTTTTTTCTTAATATTATTTTCATGGTTTTGGAGGAATTTTAACATACACAGCTACACACCAAATGCAAAAACTTCACTCAAATGCAAATGCATCAATAAACCATAATTATTATTTTGTGTATTTAATTTGATGATCTAGCAAAGACAATGTCTATTAACACTAACTCGTCACAGATTTAAATACTTACATATGTGAAAGAAAGAAAGAGAAAAAGAAAAAGTAGGATTAATATGAGAGATATGGTGGCAGGGACGGGGCCAATGGTAACAGAGGCAAGAACATGCGAATCTCCAAGCCATCGCTTCAAGGGGCTGTGCTTCAGTAAGAACAACTGTGGCCATGTCTGCAAAACGGAGGGCTTTCACGGCGGCCATTGCCGGGGCTTCCGTCGCCGCTGCTTCTGCACAAAGCACTGTGTTTGA

mRNA sequence

ATGAGAGATATGGTGGCAGGGACGGGGCCAATGGTAACAGAGGCAAGAACATGCGAATCTCCAAGCCATCGCTTCAAGGGGCTGTGCTTCAGTAAGAACAACTGTGGCCATGTCTGCAAAACGGAGGGCTTTCACGGCGGCCATTGCCGGGGCTTCCGTCGCCGCTGCTTCTGCACAAAGCACTGTGTTTGA

Coding sequence (CDS)

ATGAGAGATATGGTGGCAGGGACGGGGCCAATGGTAACAGAGGCAAGAACATGCGAATCTCCAAGCCATCGCTTCAAGGGGCTGTGCTTCAGTAAGAACAACTGTGGCCATGTCTGCAAAACGGAGGGCTTTCACGGCGGCCATTGCCGGGGCTTCCGTCGCCGCTGCTTCTGCACAAAGCACTGTGTTTGA
BLAST of CSPI05G02150 vs. Swiss-Prot
Match: DEF2_CAPAN (Defensin J1-2 OS=Capsicum annuum PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 6.7e-24
Identity = 49/62 (79.03%), Postives = 53/62 (85.48%), Query Frame = 1

Query: 1  MRDMVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTK 60
          M  +V  TG MV EARTCES SHRFKGLCFSK+NCG VC TEGF+GGHCRGFRRRCFCT+
Sbjct: 14 MMMLVFATG-MVAEARTCESQSHRFKGLCFSKSNCGSVCHTEGFNGGHCRGFRRRCFCTR 73

Query: 61 HC 63
          HC
Sbjct: 74 HC 74

BLAST of CSPI05G02150 vs. Swiss-Prot
Match: DEF02_ARATH (Defensin-like protein 2 OS=Arabidopsis thaliana GN=PDF2.2 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.6e-20
Identity = 40/59 (67.80%), Postives = 46/59 (77.97%), Query Frame = 1

Query: 4  MVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          +  G GP+  EARTCES SHRFKG C S +NC +VC  EGF GG+CRGFRRRCFCT+HC
Sbjct: 19 VATGMGPVTVEARTCESQSHRFKGTCVSASNCANVCHNEGFVGGNCRGFRRRCFCTRHC 77

BLAST of CSPI05G02150 vs. Swiss-Prot
Match: DEF01_ARATH (Defensin-like protein 1 OS=Arabidopsis thaliana GN=PDF2.3 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.3e-19
Identity = 39/59 (66.10%), Postives = 45/59 (76.27%), Query Frame = 1

Query: 4  MVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          +  G GP+  EARTCES SHRFKG C S +NC +VC  EGF GG CRGFRRRC+CT+HC
Sbjct: 19 IATGMGPVTVEARTCESKSHRFKGPCVSTHNCANVCHNEGFGGGKCRGFRRRCYCTRHC 77

BLAST of CSPI05G02150 vs. Swiss-Prot
Match: DFSD2_HELAN (Defensin SD2 OS=Helianthus annuus GN=SD2 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.4e-18
Identity = 38/54 (70.37%), Postives = 42/54 (77.78%), Query Frame = 1

Query: 9  GPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          GP+V EARTCES SH+FKG C S  NC +VC +E F GG CRGFRRRCFCT HC
Sbjct: 25 GPLVVEARTCESQSHKFKGTCLSDTNCANVCHSERFSGGKCRGFRRRCFCTTHC 78

BLAST of CSPI05G02150 vs. Swiss-Prot
Match: DEF04_ARATH (Defensin-like protein 4 OS=Arabidopsis thaliana GN=PDF2.1 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 2.7e-17
Identity = 37/59 (62.71%), Postives = 42/59 (71.19%), Query Frame = 1

Query: 4  MVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          +  G GP+  EARTC S S RFKG C S  NC +VC  EGF GG CRGFRRRCFCT++C
Sbjct: 19 VATGMGPVTVEARTCASQSQRFKGKCVSDTNCENVCHNEGFPGGDCRGFRRRCFCTRNC 77

BLAST of CSPI05G02150 vs. TrEMBL
Match: A0A0A0KJ50_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G114570 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 6.3e-29
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 1

Query: 7  GTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 64
          G GPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV
Sbjct: 18 GMGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

BLAST of CSPI05G02150 vs. TrEMBL
Match: V5LEQ8_CITLA (Defensin-like protein 1 OS=Citrullus lanatus GN=PDF2.1 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 2.0e-27
Identity = 53/60 (88.33%), Postives = 55/60 (91.67%), Query Frame = 1

Query: 4  MVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 63
          +  G GPMVT+ARTCESPS  FKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV
Sbjct: 15 LATGMGPMVTDARTCESPSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

BLAST of CSPI05G02150 vs. TrEMBL
Match: A0A0C4FSU3_TOBAC (DEF2 protein OS=Nicotiana tabacum GN=DEF2 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 3.3e-22
Identity = 46/54 (85.19%), Postives = 49/54 (90.74%), Query Frame = 1

Query: 9  GPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          GPMV EARTCES SHRFKGLCFSK+NC  VC TEGF+GGHCRGFRRRCFCT+HC
Sbjct: 24 GPMVAEARTCESQSHRFKGLCFSKSNCASVCHTEGFYGGHCRGFRRRCFCTRHC 77

BLAST of CSPI05G02150 vs. TrEMBL
Match: V5LF08_CITLA (Defensin-like protein 4 OS=Citrullus lanatus GN=PDF2.4 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.8e-21
Identity = 47/59 (79.66%), Postives = 50/59 (84.75%), Query Frame = 1

Query: 7  GTG--PMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 64
          GTG  P+V EAR CES SH FKGLCFS  NCG++CKTEGF GGHCRGFRRRCFCTKHCV
Sbjct: 17 GTGMEPIVVEARVCESLSHSFKGLCFSSTNCGNICKTEGFTGGHCRGFRRRCFCTKHCV 75

BLAST of CSPI05G02150 vs. TrEMBL
Match: A0A0V0GKJ2_SOLCH (Putative defensin J1-2-like OS=Solanum chacoense PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.4e-20
Identity = 46/62 (74.19%), Postives = 51/62 (82.26%), Query Frame = 1

Query: 1  MRDMVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTK 60
          M  ++  TG M+ EARTCES S RFKGLCFSK+NCG VC TEGF GGHCRGFRRRCFCT+
Sbjct: 14 MLTLIFSTG-MIAEARTCESQSRRFKGLCFSKSNCGSVCHTEGFSGGHCRGFRRRCFCTR 73

Query: 61 HC 63
          HC
Sbjct: 74 HC 74

BLAST of CSPI05G02150 vs. TAIR10
Match: AT2G02100.1 (AT2G02100.1 low-molecular-weight cysteine-rich 69)

HSP 1 Score: 98.6 bits (244), Expect = 1.5e-21
Identity = 40/59 (67.80%), Postives = 46/59 (77.97%), Query Frame = 1

Query: 4  MVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          +  G GP+  EARTCES SHRFKG C S +NC +VC  EGF GG+CRGFRRRCFCT+HC
Sbjct: 19 VATGMGPVTVEARTCESQSHRFKGTCVSASNCANVCHNEGFVGGNCRGFRRRCFCTRHC 77

BLAST of CSPI05G02150 vs. TAIR10
Match: AT2G02130.1 (AT2G02130.1 low-molecular-weight cysteine-rich 68)

HSP 1 Score: 96.3 bits (238), Expect = 7.4e-21
Identity = 39/59 (66.10%), Postives = 45/59 (76.27%), Query Frame = 1

Query: 4  MVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          +  G GP+  EARTCES SHRFKG C S +NC +VC  EGF GG CRGFRRRC+CT+HC
Sbjct: 19 IATGMGPVTVEARTCESKSHRFKGPCVSTHNCANVCHNEGFGGGKCRGFRRRCYCTRHC 77

BLAST of CSPI05G02150 vs. TAIR10
Match: AT2G02120.1 (AT2G02120.1 Scorpion toxin-like knottin superfamily protein)

HSP 1 Score: 88.6 bits (218), Expect = 1.5e-18
Identity = 37/59 (62.71%), Postives = 42/59 (71.19%), Query Frame = 1

Query: 4  MVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          +  G GP+  EARTC S S RFKG C S  NC +VC  EGF GG CRGFRRRCFCT++C
Sbjct: 19 VATGMGPVTVEARTCASQSQRFKGKCVSDTNCENVCHNEGFPGGDCRGFRRRCFCTRNC 77

BLAST of CSPI05G02150 vs. TAIR10
Match: AT5G63660.1 (AT5G63660.1 Scorpion toxin-like knottin superfamily protein)

HSP 1 Score: 74.3 bits (181), Expect = 3.0e-14
Identity = 29/49 (59.18%), Postives = 35/49 (71.43%), Query Frame = 1

Query: 14 EARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          E RTC+S SH FK +C S +NC  VC+ EGF GG C GF RRC+CT+ C
Sbjct: 25 EGRTCQSKSHHFKYMCTSNHNCAIVCRNEGFSGGRCHGFHRRCYCTRLC 73

BLAST of CSPI05G02150 vs. TAIR10
Match: AT2G02140.1 (AT2G02140.1 low-molecular-weight cysteine-rich 72)

HSP 1 Score: 65.9 bits (159), Expect = 1.1e-11
Identity = 28/56 (50.00%), Postives = 38/56 (67.86%), Query Frame = 1

Query: 7  GTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          G GP+  EARTCESPS++F+G+C +  +C   C +EGF GG C     RC+C+K C
Sbjct: 22 GMGPV--EARTCESPSNKFQGVCLNSQSCAKACPSEGFSGGRCSSL--RCYCSKAC 73

BLAST of CSPI05G02150 vs. NCBI nr
Match: gi|778698443|ref|XP_011654535.1| (PREDICTED: defensin J1-2-like [Cucumis sativus])

HSP 1 Score: 134.0 bits (336), Expect = 9.0e-29
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 1

Query: 7  GTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 64
          G GPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV
Sbjct: 18 GMGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

BLAST of CSPI05G02150 vs. NCBI nr
Match: gi|659072682|ref|XP_008466730.1| (PREDICTED: defensin J1-2-like [Cucumis melo])

HSP 1 Score: 134.0 bits (336), Expect = 9.0e-29
Identity = 56/57 (98.25%), Postives = 56/57 (98.25%), Query Frame = 1

Query: 7  GTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 64
          G GPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV
Sbjct: 18 GMGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

BLAST of CSPI05G02150 vs. NCBI nr
Match: gi|558067078|gb|AHA50470.1| (defensin-like protein 1 [Citrullus lanatus])

HSP 1 Score: 129.0 bits (323), Expect = 2.9e-27
Identity = 53/60 (88.33%), Postives = 55/60 (91.67%), Query Frame = 1

Query: 4  MVAGTGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 63
          +  G GPMVT+ARTCESPS  FKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV
Sbjct: 15 LATGMGPMVTDARTCESPSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

BLAST of CSPI05G02150 vs. NCBI nr
Match: gi|392621850|gb|AFM82500.1| (defensin [Nicotiana tabacum])

HSP 1 Score: 111.7 bits (278), Expect = 4.8e-22
Identity = 46/54 (85.19%), Postives = 49/54 (90.74%), Query Frame = 1

Query: 9  GPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          GPMV EARTCES SHRFKGLCFSK+NC  VC TEGF+GGHCRGFRRRCFCT+HC
Sbjct: 24 GPMVAEARTCESQSHRFKGLCFSKSNCASVCHTEGFYGGHCRGFRRRCFCTRHC 77

BLAST of CSPI05G02150 vs. NCBI nr
Match: gi|697112663|ref|XP_009610211.1| (PREDICTED: defensin J1-2 [Nicotiana tomentosiformis])

HSP 1 Score: 111.7 bits (278), Expect = 4.8e-22
Identity = 46/54 (85.19%), Postives = 49/54 (90.74%), Query Frame = 1

Query: 9  GPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 63
          GPMV EARTCES SHRFKGLCFSK+NC  VC TEGF+GGHCRGFRRRCFCT+HC
Sbjct: 24 GPMVAEARTCESQSHRFKGLCFSKSNCASVCHTEGFYGGHCRGFRRRCFCTRHC 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
DEF2_CAPAN6.7e-2479.03Defensin J1-2 OS=Capsicum annuum PE=1 SV=1[more]
DEF02_ARATH2.6e-2067.80Defensin-like protein 2 OS=Arabidopsis thaliana GN=PDF2.2 PE=2 SV=1[more]
DEF01_ARATH1.3e-1966.10Defensin-like protein 1 OS=Arabidopsis thaliana GN=PDF2.3 PE=2 SV=1[more]
DFSD2_HELAN1.4e-1870.37Defensin SD2 OS=Helianthus annuus GN=SD2 PE=2 SV=1[more]
DEF04_ARATH2.7e-1762.71Defensin-like protein 4 OS=Arabidopsis thaliana GN=PDF2.1 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KJ50_CUCSA6.3e-2998.25Uncharacterized protein OS=Cucumis sativus GN=Csa_5G114570 PE=3 SV=1[more]
V5LEQ8_CITLA2.0e-2788.33Defensin-like protein 1 OS=Citrullus lanatus GN=PDF2.1 PE=2 SV=1[more]
A0A0C4FSU3_TOBAC3.3e-2285.19DEF2 protein OS=Nicotiana tabacum GN=DEF2 PE=2 SV=1[more]
V5LF08_CITLA2.8e-2179.66Defensin-like protein 4 OS=Citrullus lanatus GN=PDF2.4 PE=2 SV=1[more]
A0A0V0GKJ2_SOLCH1.4e-2074.19Putative defensin J1-2-like OS=Solanum chacoense PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G02100.11.5e-2167.80 low-molecular-weight cysteine-rich 69[more]
AT2G02130.17.4e-2166.10 low-molecular-weight cysteine-rich 68[more]
AT2G02120.11.5e-1862.71 Scorpion toxin-like knottin superfamily protein[more]
AT5G63660.13.0e-1459.18 Scorpion toxin-like knottin superfamily protein[more]
AT2G02140.11.1e-1150.00 low-molecular-weight cysteine-rich 72[more]
Match NameE-valueIdentityDescription
gi|778698443|ref|XP_011654535.1|9.0e-2998.25PREDICTED: defensin J1-2-like [Cucumis sativus][more]
gi|659072682|ref|XP_008466730.1|9.0e-2998.25PREDICTED: defensin J1-2-like [Cucumis melo][more]
gi|558067078|gb|AHA50470.1|2.9e-2788.33defensin-like protein 1 [Citrullus lanatus][more]
gi|392621850|gb|AFM82500.1|4.8e-2285.19defensin [Nicotiana tabacum][more]
gi|697112663|ref|XP_009610211.1|4.8e-2285.19PREDICTED: defensin J1-2 [Nicotiana tomentosiformis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003614Scorpion_toxin-like
IPR008176Defensin_plant
Vocabulary: Biological Process
TermDefinition
GO:0006952defense response
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0051704 multi-organism process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G02150.1CSPI05G02150.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003614Knottin, scorpion toxin-likeGENE3DG3DSA:3.30.30.10coord: 16..62
score: 1.2
IPR003614Knottin, scorpion toxin-likeSMARTSM00505gth_4coord: 17..62
score: 5.1
IPR003614Knottin, scorpion toxin-likeunknownSSF57095Scorpion toxin-likecoord: 16..62
score: 1.22
IPR008176Defensin, plantPRINTSPR00288PUROTHIONINcoord: 47..61
score: 7.4E-10coord: 17..32
score: 7.4
IPR008176Defensin, plantPROSITEPS00940GAMMA_THIONINcoord: 16..39
scor
NoneNo IPR availablePANTHERPTHR33147FAMILY NOT NAMEDcoord: 11..63
score: 4.7
NoneNo IPR availablePFAMPF00304Gamma-thionincoord: 16..62
score: 1.6