CSPI04G09650 (gene) Wild cucumber (PI 183967)

NameCSPI04G09650
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionKazal-type serine protease inhibitor
LocationChr4 : 7641884 .. 7642952 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTTTAACTATATGCTTTGGGAATGTAGACATTTCTTTAATAAAGAAAAGATATATTTAAAAACTAGCAGAGTTGAAGGTACAAACCTTCTCCCACAAATATGCAAAGCAAAAGAATCCCTCTCACGAATAGGGTTTCAAAATTATCATTCAATATCTACTTTCTGATTCTTCAAAGGAACTAATGGAAATTCAAATGATGAGGTTAAAGAAACGAAGCGTGGAGAGAGGAAAACGATGTAGGGCCACCTAATTCTAATTCCCCTTTCATTGTTTCTTCCTCAAACGTCAAGAAAATTCCACGACCGAGGTCTCTTCCTTCCTGCCCTAAATCTAGCGAGTTGACAAAACCCGCTCAACATGATTCTCTTCTCTACACCTCCGATTTCCATCGTTTTCTTCTTTTCACTCCTCATCTTCGTCTTTGCAGTCCGATCTGAGGATATTTCCTCCGCCATCCGCTTGCCTTCTGAAGCCACCAATAACCACGGCGATATAGATCTCTGTTCTGTGTCGGCCCCCTCTTATTGCCCCGTGAAATGCTTTAGGACGGACCCCGTCTGCGGAGTTGATGGCGTTACCTATTGGTGTGGTTGTGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAAGTCGGCAACGGGGGTTCTGCGCCTATTCCTGCCCAGGCCCTCCTTCTGGTTCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGAATCTCACGCCATTTCCATTCATTTTCCTCCTTACGACTATAGATGGATTGCTGTATGAATCCCCTTTCTTTCCATTTTCTTTTACTTCAACATTCTTTTGAGAGGATCATTACTGTTCGGCAATTGATTTCATCCTATTCTTTTGTCTATTTTACTTCTCACTCCGTGCCCTGGCTAATAATAATATATCTCCTACATAGATTCTATCCTAATCAGAATTGCTTTTCTTTTCGAGTCTGTTGCTAAACTTGTGTCACGGACATACTATTACGAATTCTTTTTCTTCACCCCAACTTCCATTAATCAATTTCTGGCT

mRNA sequence

ATGATTCTCTTCTCTACACCTCCGATTTCCATCGTTTTCTTCTTTTCACTCCTCATCTTCGTCTTTGCAGTCCGATCTGAGGATATTTCCTCCGCCATCCGCTTGCCTTCTGAAGCCACCAATAACCACGGCGATATAGATCTCTGTTCTGTGTCGGCCCCCTCTTATTGCCCCGTGAAATGCTTTAGGACGGACCCCGTCTGCGGAGTTGATGGCGTTACCTATTGGTGTGGTTGTGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAAGTCGGCAACGGGGGTTCTGCGCCTATTCCTGCCCAGGCCCTCCTTCTGGTTCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGA

Coding sequence (CDS)

ATGATTCTCTTCTCTACACCTCCGATTTCCATCGTTTTCTTCTTTTCACTCCTCATCTTCGTCTTTGCAGTCCGATCTGAGGATATTTCCTCCGCCATCCGCTTGCCTTCTGAAGCCACCAATAACCACGGCGATATAGATCTCTGTTCTGTGTCGGCCCCCTCTTATTGCCCCGTGAAATGCTTTAGGACGGACCCCGTCTGCGGAGTTGATGGCGTTACCTATTGGTGTGGTTGTGCCGATGCTCTGTGTTCAGGTGTCAAGGTCGCCAAGATGGGATTTTGTGAAGTCGGCAACGGGGGTTCTGCGCCTATTCCTGCCCAGGCCCTCCTTCTGGTTCATATTTTGTGGTTGATCATCCTTGGAGTTTCCGTCTTGTTTGGACTCTTCTGA
BLAST of CSPI04G09650 vs. TrEMBL
Match: A0A0A0L0T2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G120790 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 4.7e-63
Identity = 128/130 (98.46%), Postives = 128/130 (98.46%), Query Frame = 1

Query: 1   MILFSTPPISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVK 60
           MILFSTPPISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVK
Sbjct: 1   MILFSTPPISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVK 60

Query: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWLII 120
           CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHIL  II
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHIL-PII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 129

BLAST of CSPI04G09650 vs. TrEMBL
Match: A0A0A0L616_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G184020 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 2.0e-58
Identity = 116/130 (89.23%), Postives = 119/130 (91.54%), Query Frame = 1

Query: 1   MILFSTPPISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVK 60
           M LFS P ISI+F F LLIFVF VRSED+SSAIRLPSEATNN GD+DLC VS PS CPVK
Sbjct: 1   MTLFSRPQISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWLII 120
           CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIP QALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of CSPI04G09650 vs. TrEMBL
Match: W9QLJ4_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_001582 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.0e-37
Identity = 87/134 (64.93%), Postives = 103/134 (76.87%), Query Frame = 1

Query: 5   STPPISIV---FFFSLLIFVF---AVRSEDISSAIRLPSEATNNHGDIDLC--SVSAPSY 64
           S PP+S++     F+ L+F+    AVRS++  SAIRLPS+A       D C  S+  P  
Sbjct: 10  SNPPLSLLQTFVLFTTLLFLSSFPAVRSDEQFSAIRLPSDADTG---ADPCGKSLERPPS 69

Query: 65  CPVKCFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHIL 124
           CPVKCFRTDPVCGVDGVTYWCGCADA C+G KV+K+GFCEVGNGGSAP+ AQALLLVHI+
Sbjct: 70  CPVKCFRTDPVCGVDGVTYWCGCADARCAGTKVSKLGFCEVGNGGSAPLSAQALLLVHIV 129

Query: 125 WLIILGVSVLFGLF 131
           WLI+LG SVLFGLF
Sbjct: 130 WLIVLGFSVLFGLF 140

BLAST of CSPI04G09650 vs. TrEMBL
Match: G7K667_MEDTR (Kazal-type serine protease inhibitor OS=Medicago truncatula GN=MTR_5g068760 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 3.7e-36
Identity = 79/127 (62.20%), Postives = 94/127 (74.02%), Query Frame = 1

Query: 7   PPISIVFFFSLLIFVFAVRS---EDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVKCFR 66
           P  SI+  F  LIF+F + +    + SS +RLPS+        ++CSV+ PS CP KCFR
Sbjct: 2   PKFSIILTFIALIFIFPILTTAENEESSVLRLPSQ--------NVCSVTTPSSCPAKCFR 61

Query: 67  TDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWLIILGV 126
           TDPVCG DGVTYWCGCA+A C+G KVAK+GFCEVGNGGSA  P QALLLVHI+WLI+LG 
Sbjct: 62  TDPVCGADGVTYWCGCAEAACAGAKVAKLGFCEVGNGGSATFPGQALLLVHIVWLIVLGF 120

Query: 127 SVLFGLF 131
           SVLFG F
Sbjct: 122 SVLFGFF 120

BLAST of CSPI04G09650 vs. TrEMBL
Match: A0A059DDE6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A00973 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 4.1e-35
Identity = 81/117 (69.23%), Postives = 92/117 (78.63%), Query Frame = 1

Query: 17  LLIFVFAVRSEDI--SSAIRLPSEATNNHGDIDLCSVSA-PSYCPVKCFRTDPVCGVDGV 76
           LL F  A RSE     SAIRLP+E +++ G   LCS +A P  CPVKCFR DPVCGVDGV
Sbjct: 22  LLFFPSAARSEPQIPGSAIRLPTEKSSDGGG--LCSGAARPGSCPVKCFRADPVCGVDGV 81

Query: 77  TYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWLIILGVSVLFGLF 131
           TYWCGCADA CSGV+VAK+G+CEVGNGGS P+  QALLLVHI+WLI+LG SVLFG F
Sbjct: 82  TYWCGCADAACSGVEVAKLGYCEVGNGGSGPLSGQALLLVHIVWLIVLGFSVLFGFF 136

BLAST of CSPI04G09650 vs. TAIR10
Match: AT4G01575.1 (AT4G01575.1 serine protease inhibitor, Kazal-type family protein)

HSP 1 Score: 134.4 bits (337), Expect = 5.0e-32
Identity = 69/133 (51.88%), Postives = 91/133 (68.42%), Query Frame = 1

Query: 6   TPPISIVFFFSLLIF----VFAVRSEDISSAIRLPSEATN---NHGDIDLCS-VSAPSYC 65
           +P ++++ F  L++     VFA  S +    IRLPSE  N   N G+   C  ++ P+ C
Sbjct: 14  SPSLAVIAFLFLILLNLSSVFADPSTEGGEIIRLPSEKINGEKNRGEF--CEGIAKPASC 73

Query: 66  PVKCFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILW 125
           PV+CFR DPVCG D VTYWCGCADALC GV+V K G C+VGNG    +P QALLL+HI+W
Sbjct: 74  PVQCFRPDPVCGEDSVTYWCGCADALCHGVRVVKQGACDVGNGVGLSVPGQALLLIHIVW 133

Query: 126 LIILGVSVLFGLF 131
           +++LG S+LFGLF
Sbjct: 134 MMLLGFSILFGLF 144

BLAST of CSPI04G09650 vs. TAIR10
Match: AT3G61980.1 (AT3G61980.1 serine protease inhibitor, Kazal-type family protein)

HSP 1 Score: 108.2 bits (269), Expect = 3.8e-24
Identity = 57/122 (46.72%), Postives = 72/122 (59.02%), Query Frame = 1

Query: 9   ISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVKCFRTDPVC 68
           +SI F F +L  +    ++D     R         GD+    V     C + CFR DPVC
Sbjct: 6   LSIRFLFLVLCLIGLQAADDFPDKSR---------GDV-CPRVKDRGGCTINCFRADPVC 65

Query: 69  GVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWLIILGVSVLFG 128
           G DGVTYWCGC DA C G +V K G C+ GN GSA +P QALLL+HI+WL +LG+S+L G
Sbjct: 66  GTDGVTYWCGCPDAACHGARVVKKGACDTGNAGSASVPGQALLLIHIVWLFLLGLSLLVG 117

Query: 129 LF 131
            F
Sbjct: 126 GF 117

BLAST of CSPI04G09650 vs. NCBI nr
Match: gi|778692069|ref|XP_011653402.1| (PREDICTED: uncharacterized protein LOC101220346 [Cucumis sativus])

HSP 1 Score: 248.4 bits (633), Expect = 6.7e-63
Identity = 128/130 (98.46%), Postives = 128/130 (98.46%), Query Frame = 1

Query: 1   MILFSTPPISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVK 60
           MILFSTPPISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVK
Sbjct: 1   MILFSTPPISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVK 60

Query: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWLII 120
           CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHIL  II
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHIL-PII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 129

BLAST of CSPI04G09650 vs. NCBI nr
Match: gi|449445981|ref|XP_004140750.1| (PREDICTED: uncharacterized protein LOC101210178 [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 2.9e-58
Identity = 116/130 (89.23%), Postives = 119/130 (91.54%), Query Frame = 1

Query: 1   MILFSTPPISIVFFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVK 60
           M LFS P ISI+F F LLIFVF VRSED+SSAIRLPSEATNN GD+DLC VS PS CPVK
Sbjct: 1   MTLFSRPQISIIFLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVK 60

Query: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWLII 120
           CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIP QALLLVHILWLII
Sbjct: 61  CFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPGQALLLVHILWLII 120

Query: 121 LGVSVLFGLF 131
           LGVSVLFGLF
Sbjct: 121 LGVSVLFGLF 130

BLAST of CSPI04G09650 vs. NCBI nr
Match: gi|659077721|ref|XP_008439348.1| (PREDICTED: uncharacterized protein LOC103484162, partial [Cucumis melo])

HSP 1 Score: 218.0 bits (554), Expect = 9.7e-54
Identity = 107/118 (90.68%), Postives = 109/118 (92.37%), Query Frame = 1

Query: 13  FFFSLLIFVFAVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCPVKCFRTDPVCGVDG 72
           F F LLIFVF VRSED+SSAIRLPSEATNN GD+DLC VS PS CPVKCFRTDPVCGVDG
Sbjct: 25  FLFLLLIFVFPVRSEDVSSAIRLPSEATNNDGDVDLCPVSVPSSCPVKCFRTDPVCGVDG 84

Query: 73  VTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWLIILGVSVLFGLF 131
           VTYWCGCADALCSGVKVAKMGFCEVGNGGSA IP QALLLVHILWLIILGVSVLFGLF
Sbjct: 85  VTYWCGCADALCSGVKVAKMGFCEVGNGGSASIPGQALLLVHILWLIILGVSVLFGLF 142

BLAST of CSPI04G09650 vs. NCBI nr
Match: gi|703063339|ref|XP_010086937.1| (hypothetical protein L484_001582 [Morus notabilis])

HSP 1 Score: 163.3 bits (412), Expect = 2.8e-37
Identity = 87/134 (64.93%), Postives = 103/134 (76.87%), Query Frame = 1

Query: 5   STPPISIV---FFFSLLIFVF---AVRSEDISSAIRLPSEATNNHGDIDLC--SVSAPSY 64
           S PP+S++     F+ L+F+    AVRS++  SAIRLPS+A       D C  S+  P  
Sbjct: 10  SNPPLSLLQTFVLFTTLLFLSSFPAVRSDEQFSAIRLPSDADTG---ADPCGKSLERPPS 69

Query: 65  CPVKCFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHIL 124
           CPVKCFRTDPVCGVDGVTYWCGCADA C+G KV+K+GFCEVGNGGSAP+ AQALLLVHI+
Sbjct: 70  CPVKCFRTDPVCGVDGVTYWCGCADARCAGTKVSKLGFCEVGNGGSAPLSAQALLLVHIV 129

Query: 125 WLIILGVSVLFGLF 131
           WLI+LG SVLFGLF
Sbjct: 130 WLIVLGFSVLFGLF 140

BLAST of CSPI04G09650 vs. NCBI nr
Match: gi|720100133|ref|XP_010248153.1| (PREDICTED: uncharacterized protein LOC104591052 [Nelumbo nucifera])

HSP 1 Score: 161.8 bits (408), Expect = 8.3e-37
Identity = 85/132 (64.39%), Postives = 98/132 (74.24%), Query Frame = 1

Query: 5   STPPISIVFFFSLLIFVF------AVRSEDISSAIRLPSEATNNHGDIDLCSVSAPSYCP 64
           ++ P S  F  S L+F         VRSE  SSAIRLPS+    H D DLC+ S+P  CP
Sbjct: 4   TSSPSSPFFLISFLVFFIFGLCSSLVRSELDSSAIRLPSDDAV-HAD-DLCAGSSPPSCP 63

Query: 65  VKCFRTDPVCGVDGVTYWCGCADALCSGVKVAKMGFCEVGNGGSAPIPAQALLLVHILWL 124
           V CFRTDPVCG DGVTYWCGCADA+C+G +VAK+GFCEVGNGGS P+  QALLLVHI+WL
Sbjct: 64  VNCFRTDPVCGEDGVTYWCGCADAMCAGTRVAKLGFCEVGNGGSGPVSGQALLLVHIVWL 123

Query: 125 IILGVSVLFGLF 131
           I+LG SVLFGLF
Sbjct: 124 IVLGFSVLFGLF 133

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0L0T2_CUCSA4.7e-6398.46Uncharacterized protein OS=Cucumis sativus GN=Csa_4G120790 PE=4 SV=1[more]
A0A0A0L616_CUCSA2.0e-5889.23Uncharacterized protein OS=Cucumis sativus GN=Csa_3G184020 PE=4 SV=1[more]
W9QLJ4_9ROSA2.0e-3764.93Uncharacterized protein OS=Morus notabilis GN=L484_001582 PE=4 SV=1[more]
G7K667_MEDTR3.7e-3662.20Kazal-type serine protease inhibitor OS=Medicago truncatula GN=MTR_5g068760 PE=2... [more]
A0A059DDE6_EUCGR4.1e-3569.23Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A00973 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G01575.15.0e-3251.88 serine protease inhibitor, Kazal-type family protein[more]
AT3G61980.13.8e-2446.72 serine protease inhibitor, Kazal-type family protein[more]
Match NameE-valueIdentityDescription
gi|778692069|ref|XP_011653402.1|6.7e-6398.46PREDICTED: uncharacterized protein LOC101220346 [Cucumis sativus][more]
gi|449445981|ref|XP_004140750.1|2.9e-5889.23PREDICTED: uncharacterized protein LOC101210178 [Cucumis sativus][more]
gi|659077721|ref|XP_008439348.1|9.7e-5490.68PREDICTED: uncharacterized protein LOC103484162, partial [Cucumis melo][more]
gi|703063339|ref|XP_010086937.1|2.8e-3764.93hypothetical protein L484_001582 [Morus notabilis][more]
gi|720100133|ref|XP_010248153.1|8.3e-3764.39PREDICTED: uncharacterized protein LOC104591052 [Nelumbo nucifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008233 peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G09650.1CSPI04G09650.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.60.30coord: 60..96
score: 4.
NoneNo IPR availablePANTHERPTHR34376FAMILY NOT NAMEDcoord: 11..130
score: 4.1
NoneNo IPR availablePANTHERPTHR34376:SF2SERINE PROTEASE INHIBITOR, KAZAL-TYPE FAMILY PROTEINcoord: 11..130
score: 4.1