CSPI04G08580 (gene) Wild cucumber (PI 183967)

NameCSPI04G08580
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionRhomboid-like protein
LocationChr4 : 6346605 .. 6349312 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCCTTCTCAACTCCAAAAGCCTAACTGACCCTCTAAATATATATTCCTCCCTTTTCTTCATTAGCTCAAATATACACTCAAAGGTTCAAATGATGGCTGTTGTTCCCACTTTTTTCCAAATAAGAAACTTTAACGAAACGATTTCCAAGCCTAATAAGAGAGTATGTGTTTGCAAATGGGATGTGGTGGTTTCAAAGAATAATCTACCAACTTTCGTCTCCTACTCCAAACATCATAGTTGGGAAATACATGATAACGATTCGTCTGTCATTACGATCTCGACGAGAGCGAGGAAGTTGAGAAGAGGGTTGAAAGCATTATTGCACCATGTGAGATGCAGTAATGGTTTGGATTTTGAGCACTCAAAAGTATCAGAAAAATTAAGTCAGGTTTGGGAGTGTAGTACTAATGAGAAGCAATTGAGATCACTGGGTTCTTATTTTGGAAGGCTTCAAGGTGTTGGGGATAGGAAAAATTTAGATTCTTTAAAGAAGATGAAAGTTCTTGATACAGGTCAGTTCAAAGCAAAGAAGGAGCTTCAATTGCTTGATGCTTATTTTCAAAAAGTTGATAAAGGTAAACTCTTTTTTATCCAAATTCATCTATATGTTATTGTTCTATCTTTCATGATTTATCTGTCTAAGCAACTAGATACTATTGTTTCATTGTCATTAAATTCAAAAGTTTAAGCGGATAAAATATCGCAAGTTTAGATATCTACATATTATTATCATGAATTCATTTTGTTTCTATTAGTTATAAAAAGTAAATCCTCTTTCGGTATAGTTCATACTTGATGCAAGTTGAGACACCAACTTTAATTGTTTTTTCTTTCTTTTTTTAAATGCAAATGTCAGTTATAAAAACTAGTTAAAAGTTCATAATAATTCAAGAGAAAAAGAAAAATAGAACCAACATAGAAACCTTTAAAGCATTTAATATCTTAGCAATTTGTAGCCATGTCAGAATTTGATATCTATAGCAGATACAGGTTTGCACAAACATCCTCTGTCTAGTTTTGATGAACAAGAAGAAGGCAAATTGGTGTTCACAACATCCCCTTCCGAAGATCGCGTTAAAGATGACGAATTGCAAATACGACATGTGAAATTGAGTGGTATGATTCACAAAAGCATGCCAAACAAAACTCAACTACAAGAAGAAGAAGTTTGTGATCTCTATTTGATGTAAGGTTCATTATTACGATTCATTTAACGAAAAACCAATGATTTTCTTTAAGAAAAATACGTTTCATAAGTCATCCCAAACTCATATTTAGTAACGTTTGATGCAGAATATATGTTCTAATAGGTTGATTTTGGAAATATATGTATGCAGAAGTGCTTTGGTATCTGTAAATATAGCAGTTTTCTTATTTGAAGTAGCCAGTCCAGTGAAAAACTCAGAACTACAACTCTTTTCACTTCCTTCTCTATATGGAGCAAAGATCAATGAGCTGATTCTAGTAGGAGAATGGTGGAGGCTTGTTACACCTATGTTTCTGGTACAACATTGATATAACTCGGTTCGTGTTTCCATTCTATAACTGTTTTCGACATTTTATTCCTTTGTCTTTAGAAAATCGTCGATTTAAACATAGTTTAAGTGTTTTCTCGATCTGGGCATTGAAATAATTTCTTTTGAATATACTAAAAAAGTGCCTTGCCTATATTAACCTCTTTATAAACGCAGCATTCAGGGGTTCTTCACGTTGCTCTCAGTTGTTGGACACTTCTTACATTTGGCCGCCAAGTTTGTAGAGAATATGGTCCCTTTACATTTTTCTTGATCTATGTCTTAGGAGGAGTTTCTGGTAATCTCACCAGCTTTCTTCATACTCCCGATCCAACTGTGGGGGGGACGGTAAGCTATCTCAAAAACACTTACGAGACAAAACCATGTATATTTATTGAGATGGTTATTGTTTAATGTAAACTATATATTGCACCCTTTGAGCAGGGGCCAGTGTTTGCAATGATTGGAGCTTGGCTTAGTTACCAATTCCAGAACAAAGATGTTATGACAAAGGATGTTTCAGACAAGATGTTTTTGAAAGCATTAGTTGCAGCTGTCATAAGTTCCATCTTAAGCAATATCGGTCCCATTGATGAATGGTAAGTTAAGAAAATCTCTCATTTAGCAAAGAGTGGAATAGTTTAAAGCATAAAAATGAAACAATGATGATGATGATGATGATATAGAAATGTTTGGTTAATTTGGTGGTGTGTTGTAATTAATTGTAGGACTCACACAGGAGCAGCTTTTAGTGGAATGTTATATGGGTTTCTAACATCCCCAATTGTGGAGGTCAATGATGCTTCTTCTTCTTCTTCTTCAAGAAGAGGCCAAGAAAAGGGAATAAAGCTCGTTAGGAAATATGCTAATCCTTGCAGATCATTGACTTTTTTTGTTCTTTTCATAATGGGATTCATAAGTTTGGTGTTCTTTATTCAACCTCCCTTCCACCACTTTGGACTTTTGAGTGCTCTTCAACTTTAATTTACTTTAGTTGTGGTTTGTTGAGAAGAAAATATTTGTATATATTGTCAAAATATTATATGTGCCTCTCTAGAGTGTGGTTTGCAAGGGCATGATATACAGTCAAGATAACTATATACCTTTTATTTAAGTTTTGGTTTACAATTTTGTCTCATTTTAACATAACTAATCTACTTAAACTTTAAATCCCTTTTTGGATTCATACCTG

mRNA sequence

ATGATGGCTGTTGTTCCCACTTTTTTCCAAATAAGAAACTTTAACGAAACGATTTCCAAGCCTAATAAGAGAGTATGTGTTTGCAAATGGGATGTGGTGGTTTCAAAGAATAATCTACCAACTTTCGTCTCCTACTCCAAACATCATAGTTGGGAAATACATGATAACGATTCGTCTGTCATTACGATCTCGACGAGAGCGAGGAAGTTGAGAAGAGGGTTGAAAGCATTATTGCACCATGTGAGATGCAGTAATGGTTTGGATTTTGAGCACTCAAAAGTATCAGAAAAATTAAGTCAGGTTTGGGAGTGTAGTACTAATGAGAAGCAATTGAGATCACTGGGTTCTTATTTTGGAAGGCTTCAAGGTGTTGGGGATAGGAAAAATTTAGATTCTTTAAAGAAGATGAAAGTTCTTGATACAGGTCAGTTCAAAGCAAAGAAGGAGCTTCAATTGCTTGATGCTTATTTTCAAAAAGTTGATAAAGATACAGGTTTGCACAAACATCCTCTGTCTAGTTTTGATGAACAAGAAGAAGGCAAATTGGTGTTCACAACATCCCCTTCCGAAGATCGCGTTAAAGATGACGAATTGCAAATACGACATGTGAAATTGAGTGGTATGATTCACAAAAGCATGCCAAACAAAACTCAACTACAAGAAGAAGAAGTTTGTGATCTCTATTTGATAAGTGCTTTGGTATCTGTAAATATAGCAGTTTTCTTATTTGAAGTAGCCAGTCCAGTGAAAAACTCAGAACTACAACTCTTTTCACTTCCTTCTCTATATGGAGCAAAGATCAATGAGCTGATTCTAGTAGGAGAATGGTGGAGGCTTGTTACACCTATGTTTCTGGTACAACATTGA

Coding sequence (CDS)

ATGATGGCTGTTGTTCCCACTTTTTTCCAAATAAGAAACTTTAACGAAACGATTTCCAAGCCTAATAAGAGAGTATGTGTTTGCAAATGGGATGTGGTGGTTTCAAAGAATAATCTACCAACTTTCGTCTCCTACTCCAAACATCATAGTTGGGAAATACATGATAACGATTCGTCTGTCATTACGATCTCGACGAGAGCGAGGAAGTTGAGAAGAGGGTTGAAAGCATTATTGCACCATGTGAGATGCAGTAATGGTTTGGATTTTGAGCACTCAAAAGTATCAGAAAAATTAAGTCAGGTTTGGGAGTGTAGTACTAATGAGAAGCAATTGAGATCACTGGGTTCTTATTTTGGAAGGCTTCAAGGTGTTGGGGATAGGAAAAATTTAGATTCTTTAAAGAAGATGAAAGTTCTTGATACAGGTCAGTTCAAAGCAAAGAAGGAGCTTCAATTGCTTGATGCTTATTTTCAAAAAGTTGATAAAGATACAGGTTTGCACAAACATCCTCTGTCTAGTTTTGATGAACAAGAAGAAGGCAAATTGGTGTTCACAACATCCCCTTCCGAAGATCGCGTTAAAGATGACGAATTGCAAATACGACATGTGAAATTGAGTGGTATGATTCACAAAAGCATGCCAAACAAAACTCAACTACAAGAAGAAGAAGTTTGTGATCTCTATTTGATAAGTGCTTTGGTATCTGTAAATATAGCAGTTTTCTTATTTGAAGTAGCCAGTCCAGTGAAAAACTCAGAACTACAACTCTTTTCACTTCCTTCTCTATATGGAGCAAAGATCAATGAGCTGATTCTAGTAGGAGAATGGTGGAGGCTTGTTACACCTATGTTTCTGGTACAACATTGA
BLAST of CSPI04G08580 vs. Swiss-Prot
Match: RBL9_ARATH (RHOMBOID-like protein 9, chloroplastic OS=Arabidopsis thaliana GN=RBL9 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 8.1e-74
Identity = 166/444 (37.39%), Postives = 249/444 (56.08%), Query Frame = 1

Query: 28  CKWDVVVSKNNLPTFVSYSKHHSWEIHDNDSSVITISTRARKLRRGLKALLHHVRCSNGL 87
           CK +V+   N L  F S  KH +        +  T   R  +++  +K++ +       L
Sbjct: 12  CKGEVLFDSNGLRRFSSGLKHRTM-------AEATTLGRDCRMKSYMKSIPYCRSPRRRL 71

Query: 88  DFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGRLQGVGDRKNLDSLKKMKVLDTGQFKAK 147
               +    K+++        ++L+ L SYFG+LQ   ++ ++ +   +      +    
Sbjct: 72  CLVRASSENKITK--------QRLKLLDSYFGKLQNDDEKPSISTGDDID--RKAELNVN 131

Query: 148 KELQLLDAYFQKVDKDTGLHKHPLSSFDEQEEGKLVFTTSPSEDRVKDDELQIRH-VKLS 207
           +EL  L AY  K+ KD                 K     S + D VK +   +   ++ +
Sbjct: 132 EELDSLSAYLDKLQKDA----------------KSKGLVSSTLDVVKSEGGSVASKLRKT 191

Query: 208 GMIHKSMPNKT---QLQEEEVCDLYLISALVSVNIAVFLFEVASPVKNSELQLFSLPSLY 267
           G+ + + P +    + Q E+  + Y +S L S+N+ V LFE A+PV+N+ + L SLP LY
Sbjct: 192 GIENNNSPFQQFDDEDQAEDTLNFYAVSILASINVGVCLFEAAAPVRNNNMGLLSLPLLY 251

Query: 268 GAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGG 327
           GAKIN+LIL GEWWRLVTPMFLHSG+ HVALS W LLTFG +VCR+YG FTF LIY+LGG
Sbjct: 252 GAKINDLILAGEWWRLVTPMFLHSGIPHVALSSWALLTFGPKVCRDYGLFTFCLIYILGG 311

Query: 328 VSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISS 387
           VSGN  SFLHT DPTVGGTGP FA+IGAWL  Q QNK+++  +  + +F KA++      
Sbjct: 312 VSGNFMSFLHTADPTVGGTGPAFALIGAWLVDQNQNKEMIKSNEYEDLFQKAIIMTGFGL 371

Query: 388 ILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLV----RKY 447
           ILS+ GPID+WT+ GA  +G++YGF T P++++    S       +++GI  V    +  
Sbjct: 372 ILSHFGPIDDWTNLGALIAGIVYGFFTCPVLQLGSGGSE------RQEGIVTVGPEKQNS 416

Query: 448 ANPCRSLTFFVLFIMGFISLVFFI 464
           A+PC+S   F +F+   ++ +  I
Sbjct: 432 ADPCKSFLLFTIFVAVIVTSLLLI 416

BLAST of CSPI04G08580 vs. Swiss-Prot
Match: GLUP_BACSU (Rhomboid protease GluP OS=Bacillus subtilis (strain 168) GN=gluP PE=1 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 6.1e-21
Identity = 73/229 (31.88%), Postives = 110/229 (48.03%), Query Frame = 1

Query: 185 TTSPSEDRVKDDELQIRHVK-LSGMIHKSMPNKTQ---LQEEEVCDLYLISALVSVNIAV 244
           T   SE+    +  Q+   + LS ++ +    KT+    Q  +    YL  AL    +  
Sbjct: 134 TEDCSEEHASFENAQMARERFLSLVLKQEEQRKTEAAVFQNGKPTFTYLFIALQI--LMF 193

Query: 245 FLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLL 304
           FL E+     N+E  +      +GAK N LI  GEWWRL+TP+ LH G+ H+A +   L 
Sbjct: 194 FLLEINGGSTNTETLV-----AFGAKENSLIAQGEWWRLLTPIVLHIGIAHLAFNTLALW 253

Query: 305 TFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNK 364
           + G  V R YG   F LIY+  G++G++ SF+ +P P+ G +G +F  +GA L     N+
Sbjct: 254 SVGTAVERMYGSGRFLLIYLAAGITGSIASFVFSPYPSAGASGAIFGCLGALLYVALSNR 313

Query: 365 DVMTKDVSDKMFLKALVAAVISSILSNIG------PIDEWTHTGAAFSG 404
                    KMFL+ +   +I  I+ N+G       ID   H G    G
Sbjct: 314 ---------KMFLRTIGTNIIVIIIINLGFGFAVSNIDNSGHIGGLIGG 346

BLAST of CSPI04G08580 vs. Swiss-Prot
Match: RBL6_ARATH (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana GN=RBL6 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.2e-13
Identity = 50/181 (27.62%), Postives = 91/181 (50.28%), Query Frame = 1

Query: 275 EWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHT 334
           E WRL+T M+LH+G++H+ ++ + ++ FG ++ +++G     LIY++ G  G++ S L  
Sbjct: 96  EKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL 155

Query: 335 PDP-TVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDE 394
               +VG +G +  ++GA LS    N  +    +     L  L    I+  +  +  +D 
Sbjct: 156 QKSISVGASGALLGLMGAMLSELLTNWTIYKSKLC--ALLSFLFIIAINLAIGLLPWVDN 215

Query: 395 WTHTGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLTFFVLFI 454
           + H G    G+L GF    I+ +   S     R   + G +   KY NPC+ + FFV  +
Sbjct: 216 FAHIG----GLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSKY-NPCQYVLFFVAAV 269

BLAST of CSPI04G08580 vs. Swiss-Prot
Match: RBL4_ARATH (RHOMBOID-like protein 4 OS=Arabidopsis thaliana GN=RBL4 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.4e-12
Identity = 56/206 (27.18%), Postives = 102/206 (49.51%), Query Frame = 1

Query: 258 SLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFL 317
           +L ++ G  + +++   E WRL++  +LH GV+H+ ++  TLL  G ++ RE+G     L
Sbjct: 121 TLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRIGL 180

Query: 318 IYVLGGVSGNLTSFLH-TPDPTVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKAL 377
           +Y++ G  G++ S L    + +VG +G VF ++G  LS  F N  + +  V   + L  +
Sbjct: 181 LYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIVTLVLI 240

Query: 378 VAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLV 437
           VA  +   L  +  +D + H G   +G L GF    ++ +       + R G        
Sbjct: 241 VAVNLG--LGVLPGVDNFAHIGGFATGFLLGF----VLLIRPHYGWINQRNGPGAKPHRF 300

Query: 438 RKYANPCRSLTFFVL---FIMGFISL 460
           + Y     +++  +L   FI+G ISL
Sbjct: 301 KIYQGILWTISLLILVAGFIVGLISL 320

BLAST of CSPI04G08580 vs. Swiss-Prot
Match: RBL5_ARATH (RHOMBOID-like protein 5 OS=Arabidopsis thaliana GN=RBL5 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 4.0e-12
Identity = 48/167 (28.74%), Postives = 85/167 (50.90%), Query Frame = 1

Query: 248 PVKNSELQLFSLPSL--YGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQ 307
           P+K + L   S+P+L   GA    L+  GE WRL++ ++LH G LH+  +  +L+  G +
Sbjct: 76  PIKENMLLGPSIPTLRKLGALERRLVEEGERWRLISCIWLHGGFLHLMANMISLMCIGMR 135

Query: 308 VCREYGPFTFFLIYVLGGVSGNLTSFLHTPD---PTVGGTGPVFAMIGAWLSYQFQNKDV 367
           + +E+G      +YV+ G+ G+L S L        +VG +G +F ++GA LS    N  +
Sbjct: 136 LEQEFGFMRIGALYVISGLGGSLVSCLTDSQGERVSVGASGALFGLLGAMLSELITNWTI 195

Query: 368 MTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFL 410
                +  M L  ++  V++  +  +  +D   H G   +G   GF+
Sbjct: 196 YENKCTALMTL--ILIIVLNLSVGFLPRVDNSAHFGGFLAGFFLGFV 240

BLAST of CSPI04G08580 vs. TrEMBL
Match: A0A0A0L0I7_CUCSA (Rhomboid-like protein OS=Cucumis sativus GN=Csa_4G094530 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 3.6e-270
Identity = 468/477 (98.11%), Postives = 472/477 (98.95%), Query Frame = 1

Query: 2   MAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKNNLPTFVSYSKHHSWEIHDNDSSVI 61
           MAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSK+NLPTFVSYSKHH+WEIHDNDSSV+
Sbjct: 1   MAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEIHDNDSSVV 60

Query: 62  TISTRARKLRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGRL 121
           TISTRARK RRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGRL
Sbjct: 61  TISTRARKSRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGRL 120

Query: 122 QGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKHPLSSFDEQEEGK 181
           QGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDK  GLHKHPLSSFDEQEEGK
Sbjct: 121 QGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDK--GLHKHPLSSFDEQEEGK 180

Query: 182 LVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSVNIAVF 241
           LVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVS+NIAVF
Sbjct: 181 LVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSINIAVF 240

Query: 242 LFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLT 301
           LFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLT
Sbjct: 241 LFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLT 300

Query: 302 FGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKD 361
           FGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKD
Sbjct: 301 FGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKD 360

Query: 362 VMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASS 421
           VMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASS
Sbjct: 361 VMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASS 420

Query: 422 SSSSRRGQEKGIKLVRKYANPCRSLTFFVLFIMGFISLVFFIQPPFHHFGLLSALQL 479
           SSSSRRGQEKGIKLVRKYANPCRSL FFVLFIMGFISLVFFIQPPFHHFGLLS LQL
Sbjct: 421 SSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPPFHHFGLLSVLQL 475

BLAST of CSPI04G08580 vs. TrEMBL
Match: U5FPF7_POPTR (Rhomboid-like protein OS=Populus trichocarpa GN=POPTR_0017s13870g PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 3.5e-108
Identity = 232/495 (46.87%), Postives = 314/495 (63.43%), Query Frame = 1

Query: 2   MAVVPTFFQIRNFNETISKPNKR-------VCVCKWDVVVSKNNLPTFVSYSKHHSWE-I 61
           MAVVP  ++I   + T+S  N         +C C      ++      +S     +W  I
Sbjct: 4   MAVVPICYKIPCKDHTLSIRNSARQHERGLLCACS---AFAQGGSYLSISSDVQRNWPVI 63

Query: 62  HDNDSSVITISTRARK-------LRRGLKALLHHVRCSNGLDFEH----SKVSEKLSQVW 121
           +     ++ + TR R        L +GL      ++  + +D  H     + S +   V 
Sbjct: 64  YTTADVLVKVKTRGRTNASKGTILNQGL------LKFQSVMDVPHLHKIHRTSRENLSVA 123

Query: 122 ECS----TNEKQLRSLGSYFGRLQGVGDRKNLDSL-KKMKVLDTG-QFKAKKELQLLDAY 181
            C+    TNEKQLR L SYFG+LQ      + DS  ++   LDT  Q   K+EL+ L+AY
Sbjct: 124 CCASKSGTNEKQLRLLDSYFGKLQDNVSEPSSDSCNERTDFLDTRVQINVKEELEYLNAY 183

Query: 182 FQKVDKDTGLHKHPLSSFDEQ--EEGKLVFTTSPSEDRVKDDELQIRHVK-LSGMIHKSM 241
             KVDKD  L  + LS+F+++  EE  +V   S S++  +D+E ++R  + L     +S 
Sbjct: 184 LDKVDKDANLENNVLSTFNDEATEENPIVKPISVSKESRRDNEERLRSFRNLRNQYVESG 243

Query: 242 PNKTQLQEE--EVCDLYLISALVSVNIAVFLFEVASPVKNSELQLFSLPSLYGAKINELI 301
             +++  E+  E    YLI  L S+NIAVFLFE+ASPV+NSE  LFSLP LYGAKINELI
Sbjct: 244 SRRSEALEQNYETSYFYLIGTLASINIAVFLFEIASPVRNSEFALFSLPLLYGAKINELI 303

Query: 302 LVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSF 361
           LVGEWWRLVTPMFLHSG  HV L  W+LLTFG +VCR YG FTFFLIYVLGG+SGNLTSF
Sbjct: 304 LVGEWWRLVTPMFLHSGAFHVVLGSWSLLTFGPEVCRGYGSFTFFLIYVLGGISGNLTSF 363

Query: 362 LHTPDPTVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPI 421
           LHTP+PTVGGTGPVFA+IGAWL YQ QNKDV++KD  D+MF KA++   +S ILS+ GPI
Sbjct: 364 LHTPEPTVGGTGPVFAIIGAWLIYQNQNKDVISKDDFDRMFQKAVITTAVSFILSHFGPI 423

Query: 422 DEWTHTGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLTFFVL 467
           D+WTH GA  +G++YGF T   ++++DA    SSR GQ++GI LV+++A+PC+SL  F +
Sbjct: 424 DDWTHLGAVLTGIVYGFFTCATLQLDDA----SSRSGQDEGIALVKRHADPCKSLIIFTI 483

BLAST of CSPI04G08580 vs. TrEMBL
Match: F6HSF6_VITVI (Rhomboid-like protein OS=Vitis vinifera GN=VIT_14s0006g03260 PE=3 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.7e-107
Identity = 210/390 (53.85%), Postives = 278/390 (71.28%), Query Frame = 1

Query: 90  EHSKVSEKLSQVW---ECSTNEKQLRSLGSYFGRLQGVGDRKNLDSL-KKMKVLD-TGQF 149
           E++   E L  VW   E STNEKQLR L  YFG+L    D  +L S  KK +++D +GQF
Sbjct: 13  ENASQRENLGTVWSASESSTNEKQLRLLDLYFGKLHNEDDGSSLHSSDKKTELMDQSGQF 72

Query: 150 KAKKELQLLDAYFQKVDKDTG----LHKHPLSSFDEQEEGKLVFTTSPSEDRVKDDELQI 209
           K K+ L+ L+ +  K++KD      L   P    DE +     F+ +   + V + +L+ 
Sbjct: 73  KPKEGLRSLEDHLGKINKDATSENYLPSSPCDKIDEDKHDIEPFSITEDVNPVDEGKLK- 132

Query: 210 RHVKLSGMIHKSMPNKTQLQE--EEVCDLYLISALVSVNIAVFLFEVASPVKNSELQLFS 269
            + +L      S P  ++ Q+  +E  DLYLIS LVS+NIAV LFE+ASP++NS+L+L S
Sbjct: 133 SYAELRNKDGDSGPKNSREQQPYDETSDLYLISILVSINIAVVLFELASPIRNSDLELSS 192

Query: 270 LPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFLI 329
           LP +YGAKIN+LILVGEWWRLVTPMFLHSG+ HVAL CW LLTFG QVCR YG FTFFLI
Sbjct: 193 LPLMYGAKINDLILVGEWWRLVTPMFLHSGIFHVALGCWVLLTFGPQVCRGYGSFTFFLI 252

Query: 330 YVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKALVA 389
           Y+LGG+SGNLTSFLHTPD TVGGTGPVFA+IGAW+ +Q QNKDV+ KDVS+ M+ KA++A
Sbjct: 253 YILGGISGNLTSFLHTPDLTVGGTGPVFAIIGAWVIHQIQNKDVIAKDVSESMYHKAIIA 312

Query: 390 AVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLVRK 449
             +SSILS+  PID+WTH GAAF+G+ YG+ T P +++N     +SS+ GQ++GI LVR+
Sbjct: 313 TALSSILSHFCPIDDWTHFGAAFTGIAYGYFTCPTIQLN----HTSSKTGQKEGITLVRR 372

Query: 450 YANPCRSLTFFVLFIMGFISLVFFIQPPFH 469
           YA+PC+SL  F LF++  I ++FFI+PP +
Sbjct: 373 YADPCKSLIIFTLFVLVLICVLFFIEPPLN 397

BLAST of CSPI04G08580 vs. TrEMBL
Match: M5WU58_PRUPE (Rhomboid-like protein OS=Prunus persica GN=PRUPE_ppa007489mg PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.3e-105
Identity = 204/372 (54.84%), Postives = 266/372 (71.51%), Query Frame = 1

Query: 101 VWECS---TNEKQLRSLGSYFGRLQ-GVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAY 160
           VW  S   TNEKQLRSL SY G+LQ G     +  +  +  +   GQ + KK L+ LD Y
Sbjct: 2   VWYASDSGTNEKQLRSLDSYLGKLQNGTNLPADKSNRTREPLTRNGQLRLKKGLESLDGY 61

Query: 161 FQKVDKDTGLHKHPLSSFDEQEEGKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNK 220
           F K++KD    K  LSS ++  E           D+ ++++   R+   +   H    ++
Sbjct: 62  FGKLNKDNPTEK--LSSINQDSE---------KSDK-REEQKSYRNPASTRDDHGPQSSQ 121

Query: 221 TQLQEEEVCDLYLISALVSVNIAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEW 280
             LQ  E+ DLYLIS L S+NIAVFLFE+ASPV++S+L LFSLP LYGAKIN LILVGEW
Sbjct: 122 DSLQYNEISDLYLISILGSINIAVFLFEIASPVRSSDLGLFSLPLLYGAKINGLILVGEW 181

Query: 281 WRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPD 340
           WRLVTPMFLHSG  H+ + CW L+TFG +VCR YG FTFFLIY+LGG+SGNL SFLHTP+
Sbjct: 182 WRLVTPMFLHSGFFHIVVGCWGLVTFGPKVCRGYGSFTFFLIYILGGISGNLISFLHTPE 241

Query: 341 PTVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTH 400
           PTVGGTGP+FAM+GAWL+YQ QNKD+++K+VS+ MF KA++  ++S  LS  GPID+WTH
Sbjct: 242 PTVGGTGPIFAMMGAWLTYQVQNKDIISKEVSEGMFRKAVITTLLSFTLSCFGPIDDWTH 301

Query: 401 TGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLTFFVLFIMGF 460
            GAAF+G+ YGFLT P ++++DASSS+S   GQE+GI LVR+YA+PC+SL FF LF +  
Sbjct: 302 LGAAFTGVAYGFLTCPTLQLDDASSSTS---GQEEGITLVRRYADPCKSLFFFTLFALVL 358

Query: 461 ISLVFFIQPPFH 469
             L+FF++PP +
Sbjct: 362 TCLLFFVEPPLN 358

BLAST of CSPI04G08580 vs. TrEMBL
Match: A0A061EIK6_THECC (Rhomboid-like protein OS=Theobroma cacao GN=TCM_019499 PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 2.4e-104
Identity = 205/370 (55.41%), Postives = 258/370 (69.73%), Query Frame = 1

Query: 107 NEKQLRSLGSYFGRLQGVGDRKNLDSLKKMKVL----DTGQFKAKKELQLLDAYFQKVDK 166
           NE QLR L SYFG+ Q   D K   S+   K +    D+G+   K  L  LDAY  K+D+
Sbjct: 115 NESQLRLLESYFGKRQE--DAKKTSSVSSGKTVELLDDSGESNTKMGLSSLDAYLSKLDE 174

Query: 167 DTGLHKHPLSSF---DEQEEGKLVFTTSPSEDRVKDDELQIRH---VKLSGMIHKSMPNK 226
           D+    +  SS       E   +    S SED   D+E ++R     +L  +   S  ++
Sbjct: 175 DSNSRNNVSSSIAGGQNPEANPVATPFSVSEDVKIDEEAKLRSDVGFRLRDVGSGSKRSE 234

Query: 227 TQLQEEEVCDLYLISALVSVNIAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEW 286
              Q +E  DLYLIS L S+NIAVFLFE+A+P++NS+L+LFSLPSLYGAKIN+LILVGEW
Sbjct: 235 ALQQYDEASDLYLISILASINIAVFLFEIATPIRNSDLELFSLPSLYGAKINDLILVGEW 294

Query: 287 WRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPD 346
           WRLVTPMFLHSG+LHVAL CW LL+FG QVCR YG FTFFLIY+LGG+SGNL SFLHTPD
Sbjct: 295 WRLVTPMFLHSGILHVALGCWALLSFGPQVCRRYGSFTFFLIYLLGGISGNLISFLHTPD 354

Query: 347 PTVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTH 406
           PTVGGTGPVFA+IGAWL YQ QNKDV  K +S++MF KA++   +S ILSN GPID+WTH
Sbjct: 355 PTVGGTGPVFAVIGAWLIYQIQNKDVTAKHISERMFQKAMLVTALSFILSNFGPIDDWTH 414

Query: 407 TGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLTFFVLFIMGF 466
            GAAF+G+ YG+ T P ++++DA    SSR GQE+ I LV + A+PC+SL  F  FI+ F
Sbjct: 415 FGAAFTGIAYGYFTCPTLQLDDA----SSRAGQEEQITLVGRNADPCKSLIVFTAFILVF 474

BLAST of CSPI04G08580 vs. TAIR10
Match: AT5G38510.1 (AT5G38510.1 Rhomboid-related intramembrane serine protease family protein)

HSP 1 Score: 279.3 bits (713), Expect = 4.6e-75
Identity = 166/444 (37.39%), Postives = 249/444 (56.08%), Query Frame = 1

Query: 28  CKWDVVVSKNNLPTFVSYSKHHSWEIHDNDSSVITISTRARKLRRGLKALLHHVRCSNGL 87
           CK +V+   N L  F S  KH +        +  T   R  +++  +K++ +       L
Sbjct: 12  CKGEVLFDSNGLRRFSSGLKHRTM-------AEATTLGRDCRMKSYMKSIPYCRSPRRRL 71

Query: 88  DFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGRLQGVGDRKNLDSLKKMKVLDTGQFKAK 147
               +    K+++        ++L+ L SYFG+LQ   ++ ++ +   +      +    
Sbjct: 72  CLVRASSENKITK--------QRLKLLDSYFGKLQNDDEKPSISTGDDID--RKAELNVN 131

Query: 148 KELQLLDAYFQKVDKDTGLHKHPLSSFDEQEEGKLVFTTSPSEDRVKDDELQIRH-VKLS 207
           +EL  L AY  K+ KD                 K     S + D VK +   +   ++ +
Sbjct: 132 EELDSLSAYLDKLQKDA----------------KSKGLVSSTLDVVKSEGGSVASKLRKT 191

Query: 208 GMIHKSMPNKT---QLQEEEVCDLYLISALVSVNIAVFLFEVASPVKNSELQLFSLPSLY 267
           G+ + + P +    + Q E+  + Y +S L S+N+ V LFE A+PV+N+ + L SLP LY
Sbjct: 192 GIENNNSPFQQFDDEDQAEDTLNFYAVSILASINVGVCLFEAAAPVRNNNMGLLSLPLLY 251

Query: 268 GAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGG 327
           GAKIN+LIL GEWWRLVTPMFLHSG+ HVALS W LLTFG +VCR+YG FTF LIY+LGG
Sbjct: 252 GAKINDLILAGEWWRLVTPMFLHSGIPHVALSSWALLTFGPKVCRDYGLFTFCLIYILGG 311

Query: 328 VSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISS 387
           VSGN  SFLHT DPTVGGTGP FA+IGAWL  Q QNK+++  +  + +F KA++      
Sbjct: 312 VSGNFMSFLHTADPTVGGTGPAFALIGAWLVDQNQNKEMIKSNEYEDLFQKAIIMTGFGL 371

Query: 388 ILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLV----RKY 447
           ILS+ GPID+WT+ GA  +G++YGF T P++++    S       +++GI  V    +  
Sbjct: 372 ILSHFGPIDDWTNLGALIAGIVYGFFTCPVLQLGSGGSE------RQEGIVTVGPEKQNS 416

Query: 448 ANPCRSLTFFVLFIMGFISLVFFI 464
           A+PC+S   F +F+   ++ +  I
Sbjct: 432 ADPCKSFLLFTIFVAVIVTSLLLI 416

BLAST of CSPI04G08580 vs. TAIR10
Match: AT1G12750.1 (AT1G12750.1 RHOMBOID-like protein 6)

HSP 1 Score: 79.3 bits (194), Expect = 7.0e-15
Identity = 50/181 (27.62%), Postives = 91/181 (50.28%), Query Frame = 1

Query: 275 EWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHT 334
           E WRL+T M+LH+G++H+ ++ + ++ FG ++ +++G     LIY++ G  G++ S L  
Sbjct: 96  EKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL 155

Query: 335 PDP-TVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDE 394
               +VG +G +  ++GA LS    N  +    +     L  L    I+  +  +  +D 
Sbjct: 156 QKSISVGASGALLGLMGAMLSELLTNWTIYKSKLC--ALLSFLFIIAINLAIGLLPWVDN 215

Query: 395 WTHTGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLVRKYANPCRSLTFFVLFI 454
           + H G    G+L GF    I+ +   S     R   + G +   KY NPC+ + FFV  +
Sbjct: 216 FAHIG----GLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSKY-NPCQYVLFFVAAV 269

BLAST of CSPI04G08580 vs. TAIR10
Match: AT3G53780.2 (AT3G53780.2 RHOMBOID-like protein 4)

HSP 1 Score: 75.9 bits (185), Expect = 7.7e-14
Identity = 56/206 (27.18%), Postives = 102/206 (49.51%), Query Frame = 1

Query: 258 SLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFL 317
           +L ++ G  + +++   E WRL++  +LH GV+H+ ++  TLL  G ++ RE+G     L
Sbjct: 121 TLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRIGL 180

Query: 318 IYVLGGVSGNLTSFLH-TPDPTVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKAL 377
           +Y++ G  G++ S L    + +VG +G VF ++G  LS  F N  + +  V   + L  +
Sbjct: 181 LYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIVTLVLI 240

Query: 378 VAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASSSSSSRRGQEKGIKLV 437
           VA  +   L  +  +D + H G   +G L GF    ++ +       + R G        
Sbjct: 241 VAVNLG--LGVLPGVDNFAHIGGFATGFLLGF----VLLIRPHYGWINQRNGPGAKPHRF 300

Query: 438 RKYANPCRSLTFFVL---FIMGFISL 460
           + Y     +++  +L   FI+G ISL
Sbjct: 301 KIYQGILWTISLLILVAGFIVGLISL 320

BLAST of CSPI04G08580 vs. TAIR10
Match: AT1G52580.1 (AT1G52580.1 RHOMBOID-like protein 5)

HSP 1 Score: 74.3 bits (181), Expect = 2.2e-13
Identity = 48/167 (28.74%), Postives = 85/167 (50.90%), Query Frame = 1

Query: 248 PVKNSELQLFSLPSL--YGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQ 307
           P+K + L   S+P+L   GA    L+  GE WRL++ ++LH G LH+  +  +L+  G +
Sbjct: 76  PIKENMLLGPSIPTLRKLGALERRLVEEGERWRLISCIWLHGGFLHLMANMISLMCIGMR 135

Query: 308 VCREYGPFTFFLIYVLGGVSGNLTSFLHTPD---PTVGGTGPVFAMIGAWLSYQFQNKDV 367
           + +E+G      +YV+ G+ G+L S L        +VG +G +F ++GA LS    N  +
Sbjct: 136 LEQEFGFMRIGALYVISGLGGSLVSCLTDSQGERVSVGASGALFGLLGAMLSELITNWTI 195

Query: 368 MTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFL 410
                +  M L  ++  V++  +  +  +D   H G   +G   GF+
Sbjct: 196 YENKCTALMTL--ILIIVLNLSVGFLPRVDNSAHFGGFLAGFFLGFV 240

BLAST of CSPI04G08580 vs. TAIR10
Match: AT5G07250.1 (AT5G07250.1 RHOMBOID-like protein 3)

HSP 1 Score: 74.3 bits (181), Expect = 2.2e-13
Identity = 41/153 (26.80%), Postives = 85/153 (55.56%), Query Frame = 1

Query: 258 SLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLTFGRQVCREYGPFTFFL 317
           +L  L   + ++++   E WRL+T ++LH+GV+H+  +  +L+  G ++ +++G     +
Sbjct: 116 TLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFVRIGV 175

Query: 318 IYVLGGVSGNLTSFLHTPDP-TVGGTGPVFAMIGAWLSYQFQNKDVMTKDVSDKMFLKAL 377
           IY+L G+ G++ S L   +  +VG +G +F ++G+ LS  F N  + +  ++    L  L
Sbjct: 176 IYLLSGIGGSVLSSLFIRNSISVGASGALFGLLGSMLSELFTNWTIYSNKIA--ALLTLL 235

Query: 378 VAAVISSILSNIGPIDEWTHTGAAFSGMLYGFL 410
              +I+  +  +  +D + H G   +G L GF+
Sbjct: 236 FVILINLAIGILPHVDNFAHVGGFVTGFLLGFI 266

BLAST of CSPI04G08580 vs. NCBI nr
Match: gi|778691757|ref|XP_004137292.2| (PREDICTED: uncharacterized protein LOC101206746 isoform X2 [Cucumis sativus])

HSP 1 Score: 949.5 bits (2453), Expect = 2.2e-273
Identity = 471/478 (98.54%), Postives = 475/478 (99.37%), Query Frame = 1

Query: 1   MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKNNLPTFVSYSKHHSWEIHDNDSSV 60
           MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSK+NLPTFVSYSKHH+WEIHDNDSSV
Sbjct: 1   MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEIHDNDSSV 60

Query: 61  ITISTRARKLRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR 120
           +TISTRARK RRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR
Sbjct: 61  VTISTRARKSRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR 120

Query: 121 LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKHPLSSFDEQEEG 180
           LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKHPLSSFDEQEEG
Sbjct: 121 LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKHPLSSFDEQEEG 180

Query: 181 KLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSVNIAV 240
           KLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVS+NIAV
Sbjct: 181 KLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSINIAV 240

Query: 241 FLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLL 300
           FLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLL
Sbjct: 241 FLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLL 300

Query: 301 TFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNK 360
           TFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNK
Sbjct: 301 TFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNK 360

Query: 361 DVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDAS 420
           DVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDAS
Sbjct: 361 DVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDAS 420

Query: 421 SSSSSRRGQEKGIKLVRKYANPCRSLTFFVLFIMGFISLVFFIQPPFHHFGLLSALQL 479
           SSSSSRRGQEKGIKLVRKYANPCRSL FFVLFIMGFISLVFFIQPPFHHFGLLS LQL
Sbjct: 421 SSSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPPFHHFGLLSVLQL 478

BLAST of CSPI04G08580 vs. NCBI nr
Match: gi|778691755|ref|XP_011653344.1| (PREDICTED: uncharacterized protein LOC101206746 isoform X1 [Cucumis sativus])

HSP 1 Score: 944.9 bits (2441), Expect = 5.5e-272
Identity = 471/479 (98.33%), Postives = 475/479 (99.16%), Query Frame = 1

Query: 1   MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKNNLPTFVSYSKHHSWEIHDNDSSV 60
           MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSK+NLPTFVSYSKHH+WEIHDNDSSV
Sbjct: 1   MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEIHDNDSSV 60

Query: 61  ITISTRARKLRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR 120
           +TISTRARK RRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR
Sbjct: 61  VTISTRARKSRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR 120

Query: 121 LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDK-DTGLHKHPLSSFDEQEE 180
           LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDK DTGLHKHPLSSFDEQEE
Sbjct: 121 LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKADTGLHKHPLSSFDEQEE 180

Query: 181 GKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSVNIA 240
           GKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVS+NIA
Sbjct: 181 GKLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSINIA 240

Query: 241 VFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTL 300
           VFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTL
Sbjct: 241 VFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTL 300

Query: 301 LTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQN 360
           LTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQN
Sbjct: 301 LTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQN 360

Query: 361 KDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDA 420
           KDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDA
Sbjct: 361 KDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDA 420

Query: 421 SSSSSSRRGQEKGIKLVRKYANPCRSLTFFVLFIMGFISLVFFIQPPFHHFGLLSALQL 479
           SSSSSSRRGQEKGIKLVRKYANPCRSL FFVLFIMGFISLVFFIQPPFHHFGLLS LQL
Sbjct: 421 SSSSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPPFHHFGLLSVLQL 479

BLAST of CSPI04G08580 vs. NCBI nr
Match: gi|778691760|ref|XP_011653345.1| (PREDICTED: uncharacterized protein LOC101206746 isoform X3 [Cucumis sativus])

HSP 1 Score: 940.3 bits (2429), Expect = 1.4e-270
Identity = 469/478 (98.12%), Postives = 473/478 (98.95%), Query Frame = 1

Query: 1   MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKNNLPTFVSYSKHHSWEIHDNDSSV 60
           MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSK+NLPTFVSYSKHH+WEIHDNDSSV
Sbjct: 1   MMAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEIHDNDSSV 60

Query: 61  ITISTRARKLRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR 120
           +TISTRARK RRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR
Sbjct: 61  VTISTRARKSRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGR 120

Query: 121 LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKHPLSSFDEQEEG 180
           LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDK  GLHKHPLSSFDEQEEG
Sbjct: 121 LQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDK--GLHKHPLSSFDEQEEG 180

Query: 181 KLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSVNIAV 240
           KLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVS+NIAV
Sbjct: 181 KLVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSINIAV 240

Query: 241 FLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLL 300
           FLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLL
Sbjct: 241 FLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLL 300

Query: 301 TFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNK 360
           TFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNK
Sbjct: 301 TFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNK 360

Query: 361 DVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDAS 420
           DVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDAS
Sbjct: 361 DVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDAS 420

Query: 421 SSSSSRRGQEKGIKLVRKYANPCRSLTFFVLFIMGFISLVFFIQPPFHHFGLLSALQL 479
           SSSSSRRGQEKGIKLVRKYANPCRSL FFVLFIMGFISLVFFIQPPFHHFGLLS LQL
Sbjct: 421 SSSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPPFHHFGLLSVLQL 476

BLAST of CSPI04G08580 vs. NCBI nr
Match: gi|700198483|gb|KGN53641.1| (hypothetical protein Csa_4G094530 [Cucumis sativus])

HSP 1 Score: 938.3 bits (2424), Expect = 5.2e-270
Identity = 468/477 (98.11%), Postives = 472/477 (98.95%), Query Frame = 1

Query: 2   MAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKNNLPTFVSYSKHHSWEIHDNDSSVI 61
           MAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSK+NLPTFVSYSKHH+WEIHDNDSSV+
Sbjct: 1   MAVVPTFFQIRNFNETISKPNKRVCVCKWDVVVSKDNLPTFVSYSKHHTWEIHDNDSSVV 60

Query: 62  TISTRARKLRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGRL 121
           TISTRARK RRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGRL
Sbjct: 61  TISTRARKSRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYFGRL 120

Query: 122 QGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKHPLSSFDEQEEGK 181
           QGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDK  GLHKHPLSSFDEQEEGK
Sbjct: 121 QGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDK--GLHKHPLSSFDEQEEGK 180

Query: 182 LVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSVNIAVF 241
           LVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVS+NIAVF
Sbjct: 181 LVFTTSPSEDRVKDDELQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSINIAVF 240

Query: 242 LFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLT 301
           LFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLT
Sbjct: 241 LFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCWTLLT 300

Query: 302 FGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKD 361
           FGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKD
Sbjct: 301 FGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQFQNKD 360

Query: 362 VMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASS 421
           VMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASS
Sbjct: 361 VMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVNDASS 420

Query: 422 SSSSRRGQEKGIKLVRKYANPCRSLTFFVLFIMGFISLVFFIQPPFHHFGLLSALQL 479
           SSSSRRGQEKGIKLVRKYANPCRSL FFVLFIMGFISLVFFIQPPFHHFGLLS LQL
Sbjct: 421 SSSSRRGQEKGIKLVRKYANPCRSLAFFVLFIMGFISLVFFIQPPFHHFGLLSVLQL 475

BLAST of CSPI04G08580 vs. NCBI nr
Match: gi|659111114|ref|XP_008455584.1| (PREDICTED: uncharacterized protein LOC103495725 isoform X2 [Cucumis melo])

HSP 1 Score: 852.0 bits (2200), Expect = 4.9e-244
Identity = 425/481 (88.36%), Postives = 453/481 (94.18%), Query Frame = 1

Query: 5   VPTFFQIRNFN------ETISKPNKRVCVCKWDVVVSKNNLPTFVSYSKHHSWEIHDNDS 64
           VPTFFQIRNFN      +TISKPNKRVC+CK DVVVSKNNLP  V+YSKHH+WEIH+NDS
Sbjct: 3   VPTFFQIRNFNDLLNPTQTISKPNKRVCICKCDVVVSKNNLPILVTYSKHHNWEIHNNDS 62

Query: 65  SVITISTRARKLRRGLKALLHHVRCSNGLDFEHSKVSEKLSQVWECSTNEKQLRSLGSYF 124
           SV+ ISTRARK   GLKALL HVRCSNGL+FE+SKV EKLSQVW+C+TNEKQLRSLGSYF
Sbjct: 63  SVVKISTRARKSIGGLKALLQHVRCSNGLNFEYSKVLEKLSQVWKCTTNEKQLRSLGSYF 122

Query: 125 GRLQGVGDRKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTGLHKHPLSSFDEQE 184
           GRLQG+GD+KNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDT LHKHPLSSFD+Q+
Sbjct: 123 GRLQGLGDKKNLDSLKKMKVLDTGQFKAKKELQLLDAYFQKVDKDTDLHKHPLSSFDDQQ 182

Query: 185 EGKLVFTTSPSEDRVKDDE-LQIRHVKLSGMIHKSMPNKTQLQEEEVCDLYLISALVSVN 244
           EGKLVFTTSP ED VKDD+  QI+HVKLSGM HKSMP+ TQLQEEEVCDLYLISALVS+N
Sbjct: 183 EGKLVFTTSPFEDYVKDDDKSQIQHVKLSGMTHKSMPDTTQLQEEEVCDLYLISALVSIN 242

Query: 245 IAVFLFEVASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCW 304
           IAVFLFE+ASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCW
Sbjct: 243 IAVFLFEIASPVKNSELQLFSLPSLYGAKINELILVGEWWRLVTPMFLHSGVLHVALSCW 302

Query: 305 TLLTFGRQVCREYGPFTFFLIYVLGGVSGNLTSFLHTPDPTVGGTGPVFAMIGAWLSYQF 364
           TLLTFGRQVCR+YGPFTFFLIYVLGGVSGNLTSFLHTP+PTVGGTGPVFAMIGAWLSYQF
Sbjct: 303 TLLTFGRQVCRKYGPFTFFLIYVLGGVSGNLTSFLHTPNPTVGGTGPVFAMIGAWLSYQF 362

Query: 365 QNKDVMTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPIVEVN 424
           QNKDV+TKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSP+ +VN
Sbjct: 363 QNKDVVTKDVSDKMFLKALVAAVISSILSNIGPIDEWTHTGAAFSGMLYGFLTSPLGKVN 422

Query: 425 DASSSSSSRRGQEKGIKLVRKYANPCRSLTFFVLFIMGFISLVFFIQPPFHHFGLLSALQ 479
           DA  SSSSRRGQEKGIK+VRKYANPC+S  FFVLFIMGFISLVFF+QPPFHHFGLLS +Q
Sbjct: 423 DA--SSSSRRGQEKGIKVVRKYANPCKSFAFFVLFIMGFISLVFFVQPPFHHFGLLSVIQ 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RBL9_ARATH8.1e-7437.39RHOMBOID-like protein 9, chloroplastic OS=Arabidopsis thaliana GN=RBL9 PE=2 SV=1[more]
GLUP_BACSU6.1e-2131.88Rhomboid protease GluP OS=Bacillus subtilis (strain 168) GN=gluP PE=1 SV=2[more]
RBL6_ARATH1.2e-1327.62RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana GN=RBL6 PE=2 SV=1[more]
RBL4_ARATH1.4e-1227.18RHOMBOID-like protein 4 OS=Arabidopsis thaliana GN=RBL4 PE=2 SV=1[more]
RBL5_ARATH4.0e-1228.74RHOMBOID-like protein 5 OS=Arabidopsis thaliana GN=RBL5 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L0I7_CUCSA3.6e-27098.11Rhomboid-like protein OS=Cucumis sativus GN=Csa_4G094530 PE=3 SV=1[more]
U5FPF7_POPTR3.5e-10846.87Rhomboid-like protein OS=Populus trichocarpa GN=POPTR_0017s13870g PE=3 SV=1[more]
F6HSF6_VITVI1.7e-10753.85Rhomboid-like protein OS=Vitis vinifera GN=VIT_14s0006g03260 PE=3 SV=1[more]
M5WU58_PRUPE1.3e-10554.84Rhomboid-like protein OS=Prunus persica GN=PRUPE_ppa007489mg PE=3 SV=1[more]
A0A061EIK6_THECC2.4e-10455.41Rhomboid-like protein OS=Theobroma cacao GN=TCM_019499 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G38510.14.6e-7537.39 Rhomboid-related intramembrane serine protease family protein[more]
AT1G12750.17.0e-1527.62 RHOMBOID-like protein 6[more]
AT3G53780.27.7e-1427.18 RHOMBOID-like protein 4[more]
AT1G52580.12.2e-1328.74 RHOMBOID-like protein 5[more]
AT5G07250.12.2e-1326.80 RHOMBOID-like protein 3[more]
Match NameE-valueIdentityDescription
gi|778691757|ref|XP_004137292.2|2.2e-27398.54PREDICTED: uncharacterized protein LOC101206746 isoform X2 [Cucumis sativus][more]
gi|778691755|ref|XP_011653344.1|5.5e-27298.33PREDICTED: uncharacterized protein LOC101206746 isoform X1 [Cucumis sativus][more]
gi|778691760|ref|XP_011653345.1|1.4e-27098.12PREDICTED: uncharacterized protein LOC101206746 isoform X3 [Cucumis sativus][more]
gi|700198483|gb|KGN53641.1|5.2e-27098.11hypothetical protein Csa_4G094530 [Cucumis sativus][more]
gi|659111114|ref|XP_008455584.1|4.9e-24488.36PREDICTED: uncharacterized protein LOC103495725 isoform X2 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002610Peptidase_S54_rhomboid
IPR022764Peptidase_S54_rhomboid_dom
IPR002610Peptidase_S54_rhomboid
IPR022764Peptidase_S54_rhomboid_dom
IPR002610Peptidase_S54_rhomboid
IPR002610Peptidase_S54_rhomboid
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
GO:0004252serine-type endopeptidase activity
GO:0004252serine-type endopeptidase activity
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO:0006508proteolysis
GO:0006508proteolysis
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO:0016021integral component of membrane
GO:0016021integral component of membrane
GO:0016021integral component of membrane
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G08580.4CSPI04G08580.4mRNA
CSPI04G08580.3CSPI04G08580.3mRNA
CSPI04G08580.2CSPI04G08580.2mRNA
CSPI04G08580.1CSPI04G08580.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002610Peptidase S54, rhomboidPANTHERPTHR22936RHOMBOID-RELATEDcoord: 227..285
score: 3.0
NoneNo IPR availablePANTHERPTHR22936:SF4RHOMBOID FAMILY PROTEINcoord: 227..285
score: 3.0
NoneNo IPR availableunknownSSF144091Rhomboid-likecoord: 229..285
score: 1.1

The following gene(s) are paralogous to this gene:

None