CSPI04G01650 (gene) Wild cucumber (PI 183967)

NameCSPI04G01650
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionFatty-acid desaturase
LocationChr4 : 914394 .. 916793 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTATTCCATTTCCACCTTATCTCTTTTCTTCTTCTTCTTCTTTTAACCCTTTCCTTTTCTTTCCAAAACAAACACAAACCACAACTCTGTTTCAACACTAAATCACCTCATAAATTCATTCACAAACCGACAATTTTCCCCCTTCCATTTATAAATCCCCACAGCACAATCACTTCAATTCTTCACAGTCGCCTGCTTTTTTCTTTTCCCCCGATGGCTCTTCTCAATTCTAAATTCCACCGCTTCCCATCTCTCCGCCCTTCAATCCACCGTTCACCCCTCCCCACCCTTAATTTTCCCTCCATTCCCCATGACCAATTACCCTTTCATTTTGTGGGTCGTCCTAATTTCAATTCCCTGAGGTTGAATTCCCCAATTCATATCCAAAACAGAGCAATTTCTTCGCCGACAGAAGCATTTGAGCCAGAGTCGTTGCCGCCGCCACCGGCGTCGGCGGTGGATGGGAGGAGGATTTTGTTGTCGGACGTAGTGGTGAAGCGGCGGAGGGAAGTGTTTTGGGGAAGGAAATGGAATTCGTTAGATATCGGAACGGCTGGAGTGGTGGTGGCGACGCATTTACTTACACTTTTTGCTCCTTTTCAATTCAATTGGGCTGCATTTTGGGTGGCGATTTCTTTATATATAATCACTGGTCTATTCGGTATTACCCTATCATTTCATCGGAATCTCTCTCATCGGAGCTTTAAACTTCCTAAATGGCTCGAATATCTCTTCGCTTATTGTGGAGTTCAAGCCCTTCAGGTTCTTAAAGAAGAAAGAAAAAGAATCCCCTGTTTTTCCCATGTTGTTTAGGCTTTGTTTAATGTATGCTCTGTTTGTTTCTTACACTGATTAGGTTATTTTCAACTTCGTTGCAGGGGAATCCGATCGATTGGGTTAGCACGCATAGATATCACCATCAATTTTGCGATTCCGAAAGAGACCCACATAGTCCAATTGAAGGCTTTTGGTTTAGTCATATGAGTTGGTTGTTTGATACCAATTCTGTGGTTGAGAGGGTGAGAATTCAATTCAATGGCTTGAATTTTACCCAAAAAAGTTAATGTTTTAAATTCTAATTTGGATATTGTTTTTTGGGTTAGTGTGGAGAGCCAAACAATGTTGGGGATTTGGAAAAGCAACCATTTTACAAGTTCATTCAAACTACTTATCTTCTTCATCCAATTGCTCTTGGTGCTTTACTCTATTCAATGGGTGGATTTCCTTTCATTGTTTGGGGAATGGTAAGTTTGGATTACTTATGTCCCTTTTGATGTTATTGATTTTGGGTTAATTCCACCTTTCTTAAATTTATGTGTTTCTTAAAGGATTTTAGGCCAAGTAGTCACAAAATAGGAGTAAGTAAATATACAATTGATTTGACACTTCGAATGAAAGAGACAATTAATATAAGCTAAGTTTAAACATTCTTAATTAATTCAATTGTCTTTTTCAATGAGAAAAAGAAAAAGAAAGAAAGAGTTGACTTGGGTTTGAAAGTGGGCTATGGATTGGGCTTACCCATATGGGCTTTTGTGAATCTCCATGGTCCATTGAAAGATTAGTAGAGCATGAGACCTAAAAAAAATATTAGCAGGGCAGATATAGAAACAGTAACGAGACCTGTAGTATTATCAGATATGTTATATAGCAAACTTTAGACTCGGTAATAGACTATACATGTTGGCAGTATATATAGGTTAAATTGTGATTAACCAATTATAGAACTTGTGTTGTGATTCTTAATTACAGGGTGTGAGAATAGTGTGGGTGTACCATATCACTTGGCTGGTGAATTCAGCTTGCCATGTTTGGGGAAGTCAAGCTTGGAAAACAGGAGATTTATCTAGGAACAACTGGTAACTTTTAACTTCCCATACTCCCCTTTTATATGACGATGATAAGTATTGATTAATATGTTTATTGGCGAAGGTGGGTGGCATTGCTTGCATTTGGAGAGGGATGGCACAACAACCACCACGCATTCGAGTTCTCAGCCCGACATGGCCTAGAGTGGTGGCAGCTCGACATGACATGGTACGTTGTTCGGCTTCTTCAAGCTATTGGTTTGGCCACAGATGTGAAGATACCAACACAACTTCAAAAAGAAAAATTGGCAATCATCAACACCACATGATATAAAGATATTATACTAAAACTTTAATTTTTTGTAAGCAAATCTTCAGTACCAAAAACTGTATGTCTTTCTCGTTTCGAGATTCAAATGGGTTCTGAAGGCTAAAGTATATAGAAAGAGAAGATAGCATTTCAATCGGGAGACATTGGAATTGTTCTCTCTGCACTCAACTTTACATAGCGTATGATGTATTCACTAATTTAATTAGAGAGAATTTTTTTCTCCCCATTTTTTATGAATTTTGTTGCAATACAGTTCGGT

mRNA sequence

ATGGCTCTTCTCAATTCTAAATTCCACCGCTTCCCATCTCTCCGCCCTTCAATCCACCGTTCACCCCTCCCCACCCTTAATTTTCCCTCCATTCCCCATGACCAATTACCCTTTCATTTTGTGGGTCGTCCTAATTTCAATTCCCTGAGGTTGAATTCCCCAATTCATATCCAAAACAGAGCAATTTCTTCGCCGACAGAAGCATTTGAGCCAGAGTCGTTGCCGCCGCCACCGGCGTCGGCGGTGGATGGGAGGAGGATTTTGTTGTCGGACGTAGTGGTGAAGCGGCGGAGGGAAGTGTTTTGGGGAAGGAAATGGAATTCGTTAGATATCGGAACGGCTGGAGTGGTGGTGGCGACGCATTTACTTACACTTTTTGCTCCTTTTCAATTCAATTGGGCTGCATTTTGGGTGGCGATTTCTTTATATATAATCACTGGTCTATTCGGTATTACCCTATCATTTCATCGGAATCTCTCTCATCGGAGCTTTAAACTTCCTAAATGGCTCGAATATCTCTTCGCTTATTGTGGAGTTCAAGCCCTTCAGGGGAATCCGATCGATTGGGTTAGCACGCATAGATATCACCATCAATTTTGCGATTCCGAAAGAGACCCACATAGTCCAATTGAAGGCTTTTGGTTTAGTCATATGAGTTGGTTGTTTGATACCAATTCTGTGGTTGAGAGGTGTGGAGAGCCAAACAATGTTGGGGATTTGGAAAAGCAACCATTTTACAAGTTCATTCAAACTACTTATCTTCTTCATCCAATTGCTCTTGGTGCTTTACTCTATTCAATGGGTGGATTTCCTTTCATTGTTTGGGGAATGGGTGTGAGAATAGTGTGGGTGTACCATATCACTTGGCTGGTGAATTCAGCTTGCCATGTTTGGGGAAGTCAAGCTTGGAAAACAGGAGATTTATCTAGGAACAACTGGTGGGTGGCATTGCTTGCATTTGGAGAGGGATGGCACAACAACCACCACGCATTCGAGTTCTCAGCCCGACATGGCCTAGAGTGGTGGCAGCTCGACATGACATGGTACGTTGTTCGGCTTCTTCAAGCTATTGGTTTGGCCACAGATGTGAAGATACCAACACAACTTCAAAAAGAAAAATTGGCAATCATCAACACCACATGA

Coding sequence (CDS)

ATGGCTCTTCTCAATTCTAAATTCCACCGCTTCCCATCTCTCCGCCCTTCAATCCACCGTTCACCCCTCCCCACCCTTAATTTTCCCTCCATTCCCCATGACCAATTACCCTTTCATTTTGTGGGTCGTCCTAATTTCAATTCCCTGAGGTTGAATTCCCCAATTCATATCCAAAACAGAGCAATTTCTTCGCCGACAGAAGCATTTGAGCCAGAGTCGTTGCCGCCGCCACCGGCGTCGGCGGTGGATGGGAGGAGGATTTTGTTGTCGGACGTAGTGGTGAAGCGGCGGAGGGAAGTGTTTTGGGGAAGGAAATGGAATTCGTTAGATATCGGAACGGCTGGAGTGGTGGTGGCGACGCATTTACTTACACTTTTTGCTCCTTTTCAATTCAATTGGGCTGCATTTTGGGTGGCGATTTCTTTATATATAATCACTGGTCTATTCGGTATTACCCTATCATTTCATCGGAATCTCTCTCATCGGAGCTTTAAACTTCCTAAATGGCTCGAATATCTCTTCGCTTATTGTGGAGTTCAAGCCCTTCAGGGGAATCCGATCGATTGGGTTAGCACGCATAGATATCACCATCAATTTTGCGATTCCGAAAGAGACCCACATAGTCCAATTGAAGGCTTTTGGTTTAGTCATATGAGTTGGTTGTTTGATACCAATTCTGTGGTTGAGAGGTGTGGAGAGCCAAACAATGTTGGGGATTTGGAAAAGCAACCATTTTACAAGTTCATTCAAACTACTTATCTTCTTCATCCAATTGCTCTTGGTGCTTTACTCTATTCAATGGGTGGATTTCCTTTCATTGTTTGGGGAATGGGTGTGAGAATAGTGTGGGTGTACCATATCACTTGGCTGGTGAATTCAGCTTGCCATGTTTGGGGAAGTCAAGCTTGGAAAACAGGAGATTTATCTAGGAACAACTGGTGGGTGGCATTGCTTGCATTTGGAGAGGGATGGCACAACAACCACCACGCATTCGAGTTCTCAGCCCGACATGGCCTAGAGTGGTGGCAGCTCGACATGACATGGTACGTTGTTCGGCTTCTTCAAGCTATTGGTTTGGCCACAGATGTGAAGATACCAACACAACTTCAAAAAGAAAAATTGGCAATCATCAACACCACATGA
BLAST of CSPI04G01650 vs. Swiss-Prot
Match: ADS3_ARATH (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana GN=ADS3 PE=1 SV=2)

HSP 1 Score: 538.9 bits (1387), Expect = 4.5e-152
Identity = 242/365 (66.30%), Postives = 291/365 (79.73%), Query Frame = 1

Query: 12  PSLRPSIHRSPLPTLNFPSIPHDQLPFHFVGRPNFNSLRLNSPIHIQNRAISSPTEAFEP 71
           PSL P    +  P+LNF  I             +F   +  +P    +  ++   +  + 
Sbjct: 16  PSLSPRTLNTATPSLNFTRI-------------SFTHHQKLAPFKPPSLVVAFSEKGLKR 75

Query: 72  ESLPPPPASAVDGRRILLSDVVVKRRREV-FWGRKWNSLDIGTAGVVVATHLLTLFAPFQ 131
           +      A+  D RRI+LSDV+VK++ +V +W R+W ++D G   VV++ HLL+L APFQ
Sbjct: 76  DVTTAAAATEGDYRRIMLSDVLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQ 135

Query: 132 FNWAAFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWV 191
           FNW A  VA  LYI+TGL GITLSFHRNLSH++FKLPKWLEYLFAYCG QALQGNPIDWV
Sbjct: 136 FNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWV 195

Query: 192 STHRYHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQ 251
           STHRYHHQFCDS+RDPHSP++GFWFSHM+W+FDTN++ +RCGEPNNVGDLEKQPFY+F++
Sbjct: 196 STHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFDTNTITQRCGEPNNVGDLEKQPFYRFLR 255

Query: 252 TTYLLHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSR 311
           TTY+LHP+AL   LY+MGGFPFIVWGMGVRIVWVYHITWLVNSACHVWG QAW TGDLS+
Sbjct: 256 TTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSK 315

Query: 312 NNWWVALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQ 371
           NNWWVA LAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVV+ LQAIGLATDVK+P++ Q
Sbjct: 316 NNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQ 367

Query: 372 KEKLA 376
           K+++A
Sbjct: 376 KQRMA 367

BLAST of CSPI04G01650 vs. Swiss-Prot
Match: ADS32_ARATH (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 415.2 bits (1066), Expect = 7.6e-115
Identity = 181/292 (61.99%), Postives = 225/292 (77.05%), Query Frame = 1

Query: 86  RILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYII 145
           R LLS+VVV R +  FW R W S D+    + V THLL+L APF F+W AFWV   L  I
Sbjct: 71  RRLLSEVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFI 130

Query: 146 TGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERD 205
            G+  ITLS+HRNLSHRSF LPKWLEYLFAY GV A QG+PI+WVS HRYHH+ C+++RD
Sbjct: 131 NGIC-ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRD 190

Query: 206 PHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLY 265
           PHSP +GFWFSHM+W+FDT+S++E CG   NV DL +QPFY+F+Q T LLH +A   L Y
Sbjct: 191 PHSPTQGFWFSHMAWIFDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMAYSFLFY 250

Query: 266 SMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWH 325
             GG P +VWG+G+ I    H+T+LVNS CH+WG++AW T D S+NNWWVA+L  GEGWH
Sbjct: 251 FCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEGWH 310

Query: 326 NNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAII 378
           NNHHAFEFSARHGLEWWQLD+TW ++R L+AIGLAT+VK+PT+ Q +  A++
Sbjct: 311 NNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQMKGKALV 361

BLAST of CSPI04G01650 vs. Swiss-Prot
Match: ICO5D_LIMDO (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.2e-109
Identity = 182/316 (57.59%), Postives = 230/316 (72.78%), Query Frame = 1

Query: 61  AISSPTEAFEPESLPPPPASAVDGRRILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVAT 120
           ++  P    +PE     P    D  R   S+VV++R+ + FW RKWN  DI  A  ++  
Sbjct: 36  SVLDPKIPTKPEPKTETPKPKDDLERFRTSEVVLERKAKGFWRRKWNPRDIQNAVTLLVL 95

Query: 121 HLLTLFAPFQFNWAAFWVA-ISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGV 180
           H L   APF F+W AFW++ I L   +G+ GITL FHR L+H  FKLPK +EY FAYCG 
Sbjct: 96  HALAAMAPFYFSWDAFWISFILLGFASGVLGITLCFHRCLTHGGFKLPKLVEYFFAYCGS 155

Query: 181 QALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVE-RCGEPNNVG 240
            ALQG+P++WVS HRYHHQF D+ERD HSP +GFWF H+ W+ D +  VE R G  NNV 
Sbjct: 156 LALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFWFCHIGWVLDKDLFVEKRGGRRNNVN 215

Query: 241 DLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVW 300
           DL+KQ FY+F+Q TY+ H +AL ALLY +GGFP+IVWGMG R+V+++H T+ +NS CH W
Sbjct: 216 DLKKQAFYRFLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKW 275

Query: 301 GSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIG 360
           G + W TGDLS NN +VAL AFGEGWHNNHHAFE SARHGLEWW++D+TWYV+R LQAIG
Sbjct: 276 GGRPWNTGDLSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIG 335

Query: 361 LATDVKIPTQLQKEKL 375
           LAT+VK+PT+ QK+KL
Sbjct: 336 LATNVKLPTEAQKQKL 351

BLAST of CSPI04G01650 vs. Swiss-Prot
Match: ADS1_ARATH (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.6e-109
Identity = 169/282 (59.93%), Postives = 215/282 (76.24%), Query Frame = 1

Query: 96  RRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLSF 155
           R++   W RKW  LDI  A   +  H L L APF F W A  VA+ +Y + GL GIT+S+
Sbjct: 24  RKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITVSY 83

Query: 156 HRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWF 215
           HRNL+HRSFK+PKWLEY FAYCG+ A+QG+PIDWVSTHRYHHQF DS+RDPHSP EGFWF
Sbjct: 84  HRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWF 143

Query: 216 SHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFIVW 275
           SH+ WLFDT  +VE+CG   NV DL++Q +YKF+Q T L H +  G LLY  GG  F+ W
Sbjct: 144 SHLLWLFDTGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTW 203

Query: 276 GMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFSA 335
           GMG+ +   +H+T L+NS CHVWGS+ WKT D SRN WW+++ +FGE WHNNHHAFE SA
Sbjct: 204 GMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSA 263

Query: 336 RHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAII 378
           R GLEWWQ+D++WY+VR L+ IGLATDVK+P++ Q+ ++A++
Sbjct: 264 RQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMAMV 304

BLAST of CSPI04G01650 vs. Swiss-Prot
Match: ADS2_ARATH (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2)

HSP 1 Score: 391.3 bits (1004), Expect = 1.2e-107
Identity = 166/283 (58.66%), Postives = 210/283 (74.20%), Query Frame = 1

Query: 95  KRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLS 154
           K+RR VFW R+W  LD          H L L APF F W+A WV    Y I GL GIT+S
Sbjct: 25  KKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVS 84

Query: 155 FHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFW 214
           +HRNL+HRSFK+PKWLEYL AYC + A+QG+PIDWVSTHRYHHQF DSERDPHSP EGFW
Sbjct: 85  YHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFW 144

Query: 215 FSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFIV 274
           FSH+ W++D+  +V +CG   NV DL++Q FY+F+Q T L H + LG  L+ +GG  F+ 
Sbjct: 145 FSHLLWIYDSAYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVT 204

Query: 275 WGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFS 334
           WGMGV      H+T L+NS CH+WG++ WKT D SRN WW+++ +FGE WHNNHHAFE S
Sbjct: 205 WGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESS 264

Query: 335 ARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAII 378
           AR GLEWWQ+D++WY+VR  + IGLATDVK+PT+ Q+ ++AI+
Sbjct: 265 ARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAIV 306

BLAST of CSPI04G01650 vs. TrEMBL
Match: A0A0A0KTK9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006050 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 2.0e-175
Identity = 286/287 (99.65%), Postives = 287/287 (100.00%), Query Frame = 1

Query: 94  VKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITL 153
           +KRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITL
Sbjct: 1   MKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITL 60

Query: 154 SFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGF 213
           SFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGF
Sbjct: 61  SFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGF 120

Query: 214 WFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFI 273
           WFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFI
Sbjct: 121 WFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFI 180

Query: 274 VWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEF 333
           VWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEF
Sbjct: 181 VWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEF 240

Query: 334 SARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT 381
           SARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT
Sbjct: 241 SARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT 287

BLAST of CSPI04G01650 vs. TrEMBL
Match: W9REG2_9ROSA (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase OS=Morus notabilis GN=L484_025773 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 2.1e-164
Identity = 265/322 (82.30%), Postives = 292/322 (90.68%), Query Frame = 1

Query: 57  IQNRAISSPTEAFEPESLPPPPASAVDGRRILLSDVVVKRRREVFWGRKWNSLDIGTAGV 116
           I N A    TEA EPE  PP     V G+RILLSDVVV++ R VFWGRKWNSLD+GTAGV
Sbjct: 77  IPNAAFGEATEA-EPE--PP----VVSGKRILLSDVVVEKPRRVFWGRKWNSLDMGTAGV 136

Query: 117 VVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFAY 176
           V+ATHLL+LFAPF FNW AFWVAI+LY++TGLFGITLSFHRNLSHRSFKLPKWLEYLFAY
Sbjct: 137 VLATHLLSLFAPFHFNWGAFWVAIALYVVTGLFGITLSFHRNLSHRSFKLPKWLEYLFAY 196

Query: 177 CGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPNN 236
           CGV ALQGNPIDWVSTHRYHHQFCDSERDPHSP EGFWFSHMSWLFDTNSV+ERCG PNN
Sbjct: 197 CGVLALQGNPIDWVSTHRYHHQFCDSERDPHSPTEGFWFSHMSWLFDTNSVIERCGAPNN 256

Query: 237 VGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSACH 296
           V DLEKQPFYKF++++Y+LHPIALG LLY++GGFPF+VWGMGVR++WVYHITWLVNSACH
Sbjct: 257 VSDLEKQPFYKFMRSSYILHPIALGVLLYAVGGFPFLVWGMGVRVIWVYHITWLVNSACH 316

Query: 297 VWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQA 356
           VWG QAWKTGDLSRNNWWVALLAFGEGWHNNHHAFE+SARHGLEWWQLDMTW VVRLLQA
Sbjct: 317 VWGKQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEYSARHGLEWWQLDMTWCVVRLLQA 376

Query: 357 IGLATDVKIPTQLQKEKLAIIN 379
           IG ATDVK+P + QK+++A+ N
Sbjct: 377 IGFATDVKVPNETQKQRMALNN 391

BLAST of CSPI04G01650 vs. TrEMBL
Match: B9RV72_RICCO (Delta 9 desaturase, putative OS=Ricinus communis GN=RCOM_0900580 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 1.1e-160
Identity = 254/295 (86.10%), Postives = 274/295 (92.88%), Query Frame = 1

Query: 84  GRRILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLY 143
           GRRILLSDVVVKR R VF GRKWN LDI TAGVV+A HLL+LFAPF FNW+AFWVA  LY
Sbjct: 92  GRRILLSDVVVKRERNVFLGRKWNLLDIATAGVVLAMHLLSLFAPFYFNWSAFWVAFGLY 151

Query: 144 IITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSE 203
           +ITGL GITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSE
Sbjct: 152 VITGLLGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSE 211

Query: 204 RDPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGAL 263
           RDPHSPIEGFW+SHMSWLFDTNS+++RCGEPNNVGDLEKQPFYKFIQ TY+LHPIAL  L
Sbjct: 212 RDPHSPIEGFWYSHMSWLFDTNSIIKRCGEPNNVGDLEKQPFYKFIQQTYILHPIALATL 271

Query: 264 LYSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEG 323
           LY++GGFPF+VWGMGVRI+WV+HITWLVNSACHVWG Q W TGDLSRNNWWVALLAFGEG
Sbjct: 272 LYAVGGFPFLVWGMGVRIIWVFHITWLVNSACHVWGKQEWNTGDLSRNNWWVALLAFGEG 331

Query: 324 WHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIIN 379
           WHNNHHAFE+SARHGLEWWQ+DMTWYVVRLL+AIGLATDVK+P + QK+K A  N
Sbjct: 332 WHNNHHAFEYSARHGLEWWQIDMTWYVVRLLEAIGLATDVKVPNETQKQKKAFNN 386

BLAST of CSPI04G01650 vs. TrEMBL
Match: A0A067KLF1_JATCU (Fatty acid desaturase 5 OS=Jatropha curcas GN=JCGZ_06132 PE=2 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.3e-158
Identity = 246/294 (83.67%), Postives = 273/294 (92.86%), Query Frame = 1

Query: 85  RRILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYI 144
           RRILLSDVVV R R VFWGRKWNS+DIGTAGVV+ATHLL+L APF FNW+AFWVA  LY 
Sbjct: 78  RRILLSDVVVTRERNVFWGRKWNSMDIGTAGVVLATHLLSLLAPFYFNWSAFWVAFGLYC 137

Query: 145 ITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSER 204
           +TGL GITLSFHRNLSHRSFKLPKWLEYLFAYCGVQA QGNPIDWVSTHRYHHQFCDSER
Sbjct: 138 VTGLLGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQAFQGNPIDWVSTHRYHHQFCDSER 197

Query: 205 DPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALL 264
           DPHSP+EGFW+SH+SW+FD++S++ERCG   NVGDLEKQPFYKFIQ TY+LHPIALG LL
Sbjct: 198 DPHSPVEGFWYSHISWMFDSSSIIERCGGQTNVGDLEKQPFYKFIQNTYILHPIALGVLL 257

Query: 265 YSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGW 324
           Y++GGFP++VWGMGVRI+WV+HITWLVNSACHVWG QAW TGDLSRNNWWVALLAFGEGW
Sbjct: 258 YAIGGFPYLVWGMGVRIIWVFHITWLVNSACHVWGKQAWNTGDLSRNNWWVALLAFGEGW 317

Query: 325 HNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIIN 379
           HNNHHAFE+SARHGLEWWQ+DMTWYVVR LQAIGLATDVKIP ++QK+++A  N
Sbjct: 318 HNNHHAFEYSARHGLEWWQIDMTWYVVRFLQAIGLATDVKIPNEIQKQRMAFNN 371

BLAST of CSPI04G01650 vs. TrEMBL
Match: D3YLK2_VERFO (Delta-9 fatty acid desaturase OS=Vernicia fordii PE=2 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.3e-158
Identity = 246/294 (83.67%), Postives = 275/294 (93.54%), Query Frame = 1

Query: 85  RRILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYI 144
           RRILLSDV VKR R+VF GRKWN+LDI TAGVV+A HLL+LFAPF FNW+AFWVA  LY+
Sbjct: 19  RRILLSDVAVKRERKVFLGRKWNTLDISTAGVVLAMHLLSLFAPFYFNWSAFWVAFGLYV 78

Query: 145 ITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSER 204
           +TGL GITLSFHRNLSHRSFKLPKWLEY FAYCGVQALQGNPIDWVSTHRYHHQFCDSER
Sbjct: 79  VTGLLGITLSFHRNLSHRSFKLPKWLEYSFAYCGVQALQGNPIDWVSTHRYHHQFCDSER 138

Query: 205 DPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALL 264
           DPHSPIEGFW+SHMSWLFDT++++ERCG PNNVGDLEKQPFYKFIQ TY+ HPIALG LL
Sbjct: 139 DPHSPIEGFWYSHMSWLFDTSAIIERCGGPNNVGDLEKQPFYKFIQNTYIFHPIALGVLL 198

Query: 265 YSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGW 324
           Y++GGFPF+VWGMGVRI+WV+HITWLVNSACHVWG QAW TGDLSRNNWWVA+LAFGEGW
Sbjct: 199 YAIGGFPFLVWGMGVRIIWVFHITWLVNSACHVWGKQAWNTGDLSRNNWWVAVLAFGEGW 258

Query: 325 HNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIIN 379
           HNNHHAFE+SARHGLEWWQ+DMTWYVVR L+AIGLATDVK+P+++QK+++A  N
Sbjct: 259 HNNHHAFEYSARHGLEWWQIDMTWYVVRFLEAIGLATDVKVPSEMQKQRMAFNN 312

BLAST of CSPI04G01650 vs. TAIR10
Match: AT3G15850.1 (AT3G15850.1 fatty acid desaturase 5)

HSP 1 Score: 538.9 bits (1387), Expect = 2.6e-153
Identity = 242/365 (66.30%), Postives = 291/365 (79.73%), Query Frame = 1

Query: 12  PSLRPSIHRSPLPTLNFPSIPHDQLPFHFVGRPNFNSLRLNSPIHIQNRAISSPTEAFEP 71
           PSL P    +  P+LNF  I             +F   +  +P    +  ++   +  + 
Sbjct: 16  PSLSPRTLNTATPSLNFTRI-------------SFTHHQKLAPFKPPSLVVAFSEKGLKR 75

Query: 72  ESLPPPPASAVDGRRILLSDVVVKRRREV-FWGRKWNSLDIGTAGVVVATHLLTLFAPFQ 131
           +      A+  D RRI+LSDV+VK++ +V +W R+W ++D G   VV++ HLL+L APFQ
Sbjct: 76  DVTTAAAATEGDYRRIMLSDVLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQ 135

Query: 132 FNWAAFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWV 191
           FNW A  VA  LYI+TGL GITLSFHRNLSH++FKLPKWLEYLFAYCG QALQGNPIDWV
Sbjct: 136 FNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWV 195

Query: 192 STHRYHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQ 251
           STHRYHHQFCDS+RDPHSP++GFWFSHM+W+FDTN++ +RCGEPNNVGDLEKQPFY+F++
Sbjct: 196 STHRYHHQFCDSDRDPHSPLDGFWFSHMNWMFDTNTITQRCGEPNNVGDLEKQPFYRFLR 255

Query: 252 TTYLLHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSR 311
           TTY+LHP+AL   LY+MGGFPFIVWGMGVRIVWVYHITWLVNSACHVWG QAW TGDLS+
Sbjct: 256 TTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLSK 315

Query: 312 NNWWVALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQ 371
           NNWWVA LAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVV+ LQAIGLATDVK+P++ Q
Sbjct: 316 NNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEAQ 367

Query: 372 KEKLA 376
           K+++A
Sbjct: 376 KQRMA 367

BLAST of CSPI04G01650 vs. TAIR10
Match: AT3G15870.1 (AT3G15870.1 Fatty acid desaturase family protein)

HSP 1 Score: 415.2 bits (1066), Expect = 4.3e-116
Identity = 181/292 (61.99%), Postives = 225/292 (77.05%), Query Frame = 1

Query: 86  RILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYII 145
           R LLS+VVV R +  FW R W S D+    + V THLL+L APF F+W AFWV   L  I
Sbjct: 71  RRLLSEVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFI 130

Query: 146 TGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERD 205
            G+  ITLS+HRNLSHRSF LPKWLEYLFAY GV A QG+PI+WVS HRYHH+ C+++RD
Sbjct: 131 NGIC-ITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRD 190

Query: 206 PHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLY 265
           PHSP +GFWFSHM+W+FDT+S++E CG   NV DL +QPFY+F+Q T LLH +A   L Y
Sbjct: 191 PHSPTQGFWFSHMAWIFDTSSILENCGGEENVDDLVRQPFYRFLQRTVLLHMMAYSFLFY 250

Query: 266 SMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWH 325
             GG P +VWG+G+ I    H+T+LVNS CH+WG++AW T D S+NNWWVA+L  GEGWH
Sbjct: 251 FCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFSKNNWWVAILTLGEGWH 310

Query: 326 NNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAII 378
           NNHHAFEFSARHGLEWWQLD+TW ++R L+AIGLAT+VK+PT+ Q +  A++
Sbjct: 311 NNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTETQMKGKALV 361

BLAST of CSPI04G01650 vs. TAIR10
Match: AT1G06080.1 (AT1G06080.1 delta 9 desaturase 1)

HSP 1 Score: 397.5 bits (1020), Expect = 9.2e-111
Identity = 169/282 (59.93%), Postives = 215/282 (76.24%), Query Frame = 1

Query: 96  RRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLSF 155
           R++   W RKW  LDI  A   +  H L L APF F W A  VA+ +Y + GL GIT+S+
Sbjct: 24  RKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGL-GITVSY 83

Query: 156 HRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWF 215
           HRNL+HRSFK+PKWLEY FAYCG+ A+QG+PIDWVSTHRYHHQF DS+RDPHSP EGFWF
Sbjct: 84  HRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWF 143

Query: 216 SHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFIVW 275
           SH+ WLFDT  +VE+CG   NV DL++Q +YKF+Q T L H +  G LLY  GG  F+ W
Sbjct: 144 SHLLWLFDTGYLVEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTW 203

Query: 276 GMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFSA 335
           GMG+ +   +H+T L+NS CHVWGS+ WKT D SRN WW+++ +FGE WHNNHHAFE SA
Sbjct: 204 GMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSA 263

Query: 336 RHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAII 378
           R GLEWWQ+D++WY+VR L+ IGLATDVK+P++ Q+ ++A++
Sbjct: 264 RQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQRRRMAMV 304

BLAST of CSPI04G01650 vs. TAIR10
Match: AT2G31360.1 (AT2G31360.1 16:0delta9 desaturase 2)

HSP 1 Score: 391.3 bits (1004), Expect = 6.6e-109
Identity = 166/283 (58.66%), Postives = 210/283 (74.20%), Query Frame = 1

Query: 95  KRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLS 154
           K+RR VFW R+W  LD          H L L APF F W+A WV    Y I GL GIT+S
Sbjct: 25  KKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGL-GITVS 84

Query: 155 FHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFW 214
           +HRNL+HRSFK+PKWLEYL AYC + A+QG+PIDWVSTHRYHHQF DSERDPHSP EGFW
Sbjct: 85  YHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFW 144

Query: 215 FSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFIV 274
           FSH+ W++D+  +V +CG   NV DL++Q FY+F+Q T L H + LG  L+ +GG  F+ 
Sbjct: 145 FSHLLWIYDSAYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVT 204

Query: 275 WGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFS 334
           WGMGV      H+T L+NS CH+WG++ WKT D SRN WW+++ +FGE WHNNHHAFE S
Sbjct: 205 WGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESS 264

Query: 335 ARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAII 378
           AR GLEWWQ+D++WY+VR  + IGLATDVK+PT+ Q+ ++AI+
Sbjct: 265 ARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAIV 306

BLAST of CSPI04G01650 vs. TAIR10
Match: AT1G06100.1 (AT1G06100.1 Fatty acid desaturase family protein)

HSP 1 Score: 379.0 bits (972), Expect = 3.4e-105
Identity = 170/283 (60.07%), Postives = 211/283 (74.56%), Query Frame = 1

Query: 94  VKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITL 153
           V++ +  +  RKW   D+G A  V   HLL L APF + W AF   I L I+T L  IT 
Sbjct: 16  VRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLC-ITF 75

Query: 154 SFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGF 213
           S+HRNL+HRSFKLPKWLEY FAY  + ALQG+P+DWVS HR+HHQF DS+RDPHSPIEGF
Sbjct: 76  SYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPIEGF 135

Query: 214 WFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFI 273
           WFSH+ W+FDT+ + E+CG  NNV DL++Q FY+F++ T +LH +A   L+Y  GG P++
Sbjct: 136 WFSHVLWIFDTDYIREKCGRRNNVMDLKQQWFYRFLKKTLVLHILAFWTLIYLWGGLPYL 195

Query: 274 VWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEF 333
            W +G   V  YH TWLVNSACH+ GSQAW+T D SRN WW+ALL  GE WHNNHHAFE 
Sbjct: 196 TWTVGFGGVIGYHGTWLVNSACHICGSQAWQTNDTSRNVWWLALLTMGESWHNNHHAFET 255

Query: 334 SARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAI 377
           SARHGLEW+QLD+TWY++   QA+GLAT+VK+PT  QK K+AI
Sbjct: 256 SARHGLEWYQLDITWYLIWFFQALGLATNVKLPTDAQKRKMAI 297

BLAST of CSPI04G01650 vs. NCBI nr
Match: gi|659108483|ref|XP_008454223.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 770.8 bits (1989), Expect = 1.1e-219
Identity = 364/385 (94.55%), Postives = 370/385 (96.10%), Query Frame = 1

Query: 1   MALLN-----SKFHRFPSLRPSIHRSPLPTLNFPSIPHDQLPFHFVGRPNFNSLRLNSPI 60
           MALLN     SKFHRFPSLRPSIHR  LP LNFPSIPH+QLPF+FVG  NF SLRLNSPI
Sbjct: 1   MALLNPPPSHSKFHRFPSLRPSIHRQRLPALNFPSIPHEQLPFNFVGPRNFISLRLNSPI 60

Query: 61  HIQNRAISSPTEAFEPESLPPPPASAVDGRRILLSDVVVKRRREVFWGRKWNSLDIGTAG 120
           HIQNRAISSPTEAFEPESLPPP AS VDGRRILLSDVVVKRRREVFWGRKW SLDIGTAG
Sbjct: 61  HIQNRAISSPTEAFEPESLPPPLASGVDGRRILLSDVVVKRRREVFWGRKWTSLDIGTAG 120

Query: 121 VVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFA 180
           VVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFA
Sbjct: 121 VVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFA 180

Query: 181 YCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPN 240
           YCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPN
Sbjct: 181 YCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPN 240

Query: 241 NVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSAC 300
           NVGDL+KQPFYKFIQTTYLLHPIAL ALLY+MGGFPFIVWGMGVRIVWVYHITWLVNSAC
Sbjct: 241 NVGDLQKQPFYKFIQTTYLLHPIALAALLYTMGGFPFIVWGMGVRIVWVYHITWLVNSAC 300

Query: 301 HVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQ 360
           HVWG+QAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQ
Sbjct: 301 HVWGNQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQ 360

Query: 361 AIGLATDVKIPTQLQKEKLAIINTT 381
           A+GLATDVKIPTQLQKEKLAIINTT
Sbjct: 361 ALGLATDVKIPTQLQKEKLAIINTT 385

BLAST of CSPI04G01650 vs. NCBI nr
Match: gi|778730885|ref|XP_011659875.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like, partial [Cucumis sativus])

HSP 1 Score: 661.4 bits (1705), Expect = 9.7e-187
Identity = 306/306 (100.00%), Postives = 306/306 (100.00%), Query Frame = 1

Query: 75  PPPPASAVDGRRILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWA 134
           PPPPASAVDGRRILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWA
Sbjct: 1   PPPPASAVDGRRILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWA 60

Query: 135 AFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHR 194
           AFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHR
Sbjct: 61  AFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHR 120

Query: 195 YHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYL 254
           YHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYL
Sbjct: 121 YHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYL 180

Query: 255 LHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWW 314
           LHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWW
Sbjct: 181 LHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWW 240

Query: 315 VALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKL 374
           VALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKL
Sbjct: 241 VALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKL 300

Query: 375 AIINTT 381
           AIINTT
Sbjct: 301 AIINTT 306

BLAST of CSPI04G01650 vs. NCBI nr
Match: gi|778697414|ref|XP_004152346.2| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 629.4 bits (1622), Expect = 4.1e-177
Identity = 290/296 (97.97%), Postives = 293/296 (98.99%), Query Frame = 1

Query: 85  RRILLSDVVVKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYI 144
           + ILLS+  +KRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYI
Sbjct: 7   KMILLSEKGMKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYI 66

Query: 145 ITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSER 204
           ITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSER
Sbjct: 67  ITGLFGITLSFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSER 126

Query: 205 DPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALL 264
           DPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALL
Sbjct: 127 DPHSPIEGFWFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALL 186

Query: 265 YSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGW 324
           YSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGW
Sbjct: 187 YSMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGW 246

Query: 325 HNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT 381
           HNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT
Sbjct: 247 HNNHHAFEFSARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT 302

BLAST of CSPI04G01650 vs. NCBI nr
Match: gi|700197740|gb|KGN52898.1| (hypothetical protein Csa_4G006050 [Cucumis sativus])

HSP 1 Score: 623.2 bits (1606), Expect = 2.9e-175
Identity = 286/287 (99.65%), Postives = 287/287 (100.00%), Query Frame = 1

Query: 94  VKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITL 153
           +KRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITL
Sbjct: 1   MKRRREVFWGRKWNSLDIGTAGVVVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITL 60

Query: 154 SFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGF 213
           SFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGF
Sbjct: 61  SFHRNLSHRSFKLPKWLEYLFAYCGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGF 120

Query: 214 WFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFI 273
           WFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFI
Sbjct: 121 WFSHMSWLFDTNSVVERCGEPNNVGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFI 180

Query: 274 VWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEF 333
           VWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEF
Sbjct: 181 VWGMGVRIVWVYHITWLVNSACHVWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEF 240

Query: 334 SARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT 381
           SARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT
Sbjct: 241 SARHGLEWWQLDMTWYVVRLLQAIGLATDVKIPTQLQKEKLAIINTT 287

BLAST of CSPI04G01650 vs. NCBI nr
Match: gi|703101574|ref|XP_010097224.1| (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase [Morus notabilis])

HSP 1 Score: 586.6 bits (1511), Expect = 3.0e-164
Identity = 265/322 (82.30%), Postives = 292/322 (90.68%), Query Frame = 1

Query: 57  IQNRAISSPTEAFEPESLPPPPASAVDGRRILLSDVVVKRRREVFWGRKWNSLDIGTAGV 116
           I N A    TEA EPE  PP     V G+RILLSDVVV++ R VFWGRKWNSLD+GTAGV
Sbjct: 77  IPNAAFGEATEA-EPE--PP----VVSGKRILLSDVVVEKPRRVFWGRKWNSLDMGTAGV 136

Query: 117 VVATHLLTLFAPFQFNWAAFWVAISLYIITGLFGITLSFHRNLSHRSFKLPKWLEYLFAY 176
           V+ATHLL+LFAPF FNW AFWVAI+LY++TGLFGITLSFHRNLSHRSFKLPKWLEYLFAY
Sbjct: 137 VLATHLLSLFAPFHFNWGAFWVAIALYVVTGLFGITLSFHRNLSHRSFKLPKWLEYLFAY 196

Query: 177 CGVQALQGNPIDWVSTHRYHHQFCDSERDPHSPIEGFWFSHMSWLFDTNSVVERCGEPNN 236
           CGV ALQGNPIDWVSTHRYHHQFCDSERDPHSP EGFWFSHMSWLFDTNSV+ERCG PNN
Sbjct: 197 CGVLALQGNPIDWVSTHRYHHQFCDSERDPHSPTEGFWFSHMSWLFDTNSVIERCGAPNN 256

Query: 237 VGDLEKQPFYKFIQTTYLLHPIALGALLYSMGGFPFIVWGMGVRIVWVYHITWLVNSACH 296
           V DLEKQPFYKF++++Y+LHPIALG LLY++GGFPF+VWGMGVR++WVYHITWLVNSACH
Sbjct: 257 VSDLEKQPFYKFMRSSYILHPIALGVLLYAVGGFPFLVWGMGVRVIWVYHITWLVNSACH 316

Query: 297 VWGSQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVRLLQA 356
           VWG QAWKTGDLSRNNWWVALLAFGEGWHNNHHAFE+SARHGLEWWQLDMTW VVRLLQA
Sbjct: 317 VWGKQAWKTGDLSRNNWWVALLAFGEGWHNNHHAFEYSARHGLEWWQLDMTWCVVRLLQA 376

Query: 357 IGLATDVKIPTQLQKEKLAIIN 379
           IG ATDVK+P + QK+++A+ N
Sbjct: 377 IGFATDVKVPNETQKQRMALNN 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ADS3_ARATH4.5e-15266.30Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
ADS32_ARATH7.6e-11561.99Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.... [more]
ICO5D_LIMDO1.2e-10957.59Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1[more]
ADS1_ARATH1.6e-10959.93Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1[more]
ADS2_ARATH1.2e-10758.66Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KTK9_CUCSA2.0e-17599.65Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006050 PE=3 SV=1[more]
W9REG2_9ROSA2.1e-16482.30Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase OS=Morus notabilis GN=... [more]
B9RV72_RICCO1.1e-16086.10Delta 9 desaturase, putative OS=Ricinus communis GN=RCOM_0900580 PE=3 SV=1[more]
A0A067KLF1_JATCU1.3e-15883.67Fatty acid desaturase 5 OS=Jatropha curcas GN=JCGZ_06132 PE=2 SV=1[more]
D3YLK2_VERFO1.3e-15883.67Delta-9 fatty acid desaturase OS=Vernicia fordii PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G15850.12.6e-15366.30 fatty acid desaturase 5[more]
AT3G15870.14.3e-11661.99 Fatty acid desaturase family protein[more]
AT1G06080.19.2e-11159.93 delta 9 desaturase 1[more]
AT2G31360.16.6e-10958.66 16:0delta9 desaturase 2[more]
AT1G06100.13.4e-10560.07 Fatty acid desaturase family protein[more]
Match NameE-valueIdentityDescription
gi|659108483|ref|XP_008454223.1|1.1e-21994.55PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|778730885|ref|XP_011659875.1|9.7e-187100.00PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|778697414|ref|XP_004152346.2|4.1e-17797.97PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|700197740|gb|KGN52898.1|2.9e-17599.65hypothetical protein Csa_4G006050 [Cucumis sativus][more]
gi|703101574|ref|XP_010097224.1|3.0e-16482.30Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005804FA_desaturase_dom
IPR015876Acyl-CoA_DS
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016717oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0010205 photoinhibition
biological_process GO:0006636 unsaturated fatty acid biosynthetic process
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0004768 stearoyl-CoA 9-desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G01650.1CSPI04G01650.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 132..352
score: 4.9
IPR015876Fatty acid desaturase, type 1, corePRINTSPR00075FACDDSATRASEcoord: 321..335
score: 1.2E-25coord: 156..176
score: 1.2E-25coord: 278..299
score: 1.2E-25coord: 193..222
score: 1.2
NoneNo IPR availablePANTHERPTHR11351ACYL-COA DESATURASEcoord: 85..377
score: 7.7E
NoneNo IPR availablePANTHERPTHR11351:SF2DELTA-9 ACYL-LIPID DESATURASE 1-RELATEDcoord: 85..377
score: 7.7E

The following gene(s) are paralogous to this gene:

None