CSPI03G10300 (gene) Wild cucumber (PI 183967)

NameCSPI03G10300
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionRing finger protein, putative
LocationChr3 : 8278505 .. 8280252 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGAAAATTACGTGTCGCTTTGTATTTTCTCATCCATTTAATCATGTGCATCTCACGTGTCTCACTCCAACTGAAATTCTCTCTTCTCTTCCTCTCTCTCTCTCTTCGTTCTCTCAACTTAATGAAACCGAGAGAGACCTGCCCGAAAAAACTGACCGCTGCATTTACACTCTTTTCCATCTCCTCCGATATTTCCCATTTCTCTCTCTCTCTCTCTCTAAAATTTAGACATATTACTGCAATGGCTTCTCCGCCGACGCCGCCATTGCCGGACGATCTTGCCATTGGCTACTTTTCATCATCTCCTCCTCCTCCTCCTTCGCCGGTCTCCAGTCACACCTCGTTGAAGAATCTCAGTCCCAGCATCTTAATCATTCTTACGATTCTTGCATTTACAGCCTTTTCTTCGGGGATTCTCTGCCTTGTTCTCCGTTACCTTAACCGACGGTGTCTTCTCCGTCTCTCTGCCTTGTCTGGATCGTCATCTTCCTCTACTGTTTCTAGTCGCCGCATTATTCCAGCAGAACAAAGTGCGGTTCGTTGTACTAACTCTTTCTCTCCAATCGAATCGCTTCCTCTGTTCTCTTTCTCCTCCGTCACGCGTCGTTCTTCCACTGCCGCCGCTGATTGCGCCGTTTGTTTGTCGAAATTTGAAGCGGAGGATCAGCTTCGTCTTCTTCCTCTCTGTTGCCATGCGTTTCACGCTCAATGCGTCGATACTTGGCTTCAATCGAACCAGAGTTGTCCTCTTTGCCGCTCCGCCATTTTCGCTTCTGAATCCGATGTAATGAAGGCATCCATGGCTTCCTATGCCGCCGAAGGTCGCGGCGGGGACAGTTTTAGGCTCGAGATTGGCAGTATTAGCCGTAGACAAGCGCCGTCCGACTCTGCCGAAGGAAGGAGATCGTATTCGATAGGATCGTTCGAATATTTCGTCGAAGAAGATTCCGAGGTAAACTTCACCAATGCTCACCGGAGGAGCGTCTCCGATAAGGAGGACATCGAAGCTCCTATATCAGCGGTATCTACAGAGCGAAGCCTAGCGGCGGAAGTCGGGTCTGGAAGGAATTGGCTGAAGGACTACGTCGATAGGCTCTCGAATTCTGTATCATCTCGTGCACTGTCTTTCCGAGGCTCCGGTAGGTTCTTCACCGGAAGTAGTCGACGGAGTGAGGTGACAGTCGCGGGAGAATGGGAACAAGAGAACAACCGCGTCGGAGAGGAAATAAGCGAGTTATTCCGGTGGTTTTCAGGGGTATGATCGTAATTACAGCACACGGGAAGAGGATTTGGAAATTTAAGGAGTTGGGTTAATCGCCGGCCGATTGGTACGCAAGTATATCTTTTTTTTGCCTTATTGAAAACGGACGGTGAAGATTCCTATTCCTTTTGATCCTTTTGATTATGTCAACGGCTGAGATTCAAGCTGGTAATCTTGTGAATAATTTTTTTTTTTCACAATTATACACCAAACATTAATTTTGTTGGCATTTAATCAAAAAGCTCAAAAAGATTGCTGAGATTTTTCTGTTACCTCGTTTTGGCTGGGATTAAAGTAAACAAAAGTTTCCCATCGTGGCAGTGAAGCCTAATACCACACTTTGAATTCATATTTTATGAAAAATGTGCCATTTTTCTTTCTATAAAAAAAAAAAAAACCATTTATCCCATTCCATAATTCCTTTCCTTTCTTTCCATATAGTTCAATGGTAATTTTGGTCAAAAGCTATAATTTATCAAATCA

mRNA sequence

ATGTGCATCTCACGTGTCTCACTCCAACTGAAATTCTCTCTTCTCTTCCTCTCTCTCTCTCTTCGTTCTCTCAACTTAATGAAACCGAGAGAGACCTGCCCGAAAAAACTGACCGCTGCATTTACACTCTTTTCCATCTCCTCCGATATTTCCCATTTCTCTCTCTCTCTCTCTCTAAAATTTAGACATATTACTGCAATGGCTTCTCCGCCGACGCCGCCATTGCCGGACGATCTTGCCATTGGCTACTTTTCATCATCTCCTCCTCCTCCTCCTTCGCCGGTCTCCAGTCACACCTCGTTGAAGAATCTCAGTCCCAGCATCTTAATCATTCTTACGATTCTTGCATTTACAGCCTTTTCTTCGGGGATTCTCTGCCTTGTTCTCCGTTACCTTAACCGACGGTGTCTTCTCCGTCTCTCTGCCTTGTCTGGATCGTCATCTTCCTCTACTGTTTCTAGTCGCCGCATTATTCCAGCAGAACAAAGTGCGGTTCGTTGTACTAACTCTTTCTCTCCAATCGAATCGCTTCCTCTGTTCTCTTTCTCCTCCGTCACGCGTCGTTCTTCCACTGCCGCCGCTGATTGCGCCGTTTGTTTGTCGAAATTTGAAGCGGAGGATCAGCTTCGTCTTCTTCCTCTCTGTTGCCATGCGTTTCACGCTCAATGCGTCGATACTTGGCTTCAATCGAACCAGAGTTGTCCTCTTTGCCGCTCCGCCATTTTCGCTTCTGAATCCGATGTAATGAAGGCATCCATGGCTTCCTATGCCGCCGAAGGTCGCGGCGGGGACAGTTTTAGGCTCGAGATTGGCAGTATTAGCCGTAGACAAGCGCCGTCCGACTCTGCCGAAGGAAGGAGATCGTATTCGATAGGATCGTTCGAATATTTCGTCGAAGAAGATTCCGAGGTAAACTTCACCAATGCTCACCGGAGGAGCGTCTCCGATAAGGAGGACATCGAAGCTCCTATATCAGCGGTATCTACAGAGCGAAGCCTAGCGGCGGAAGTCGGGTCTGGAAGGAATTGGCTGAAGGACTACGTCGATAGGCTCTCGAATTCTGTATCATCTCGTGCACTGTCTTTCCGAGGCTCCGGTAGGTTCTTCACCGGAAGTAGTCGACGGAGTGAGGTGACAGTCGCGGGAGAATGGGAACAAGAGAACAACCGCGTCGGAGAGGAAATAAGCGAGTTATTCCGGTGGTTTTCAGGGGTATGA

Coding sequence (CDS)

ATGTGCATCTCACGTGTCTCACTCCAACTGAAATTCTCTCTTCTCTTCCTCTCTCTCTCTCTTCGTTCTCTCAACTTAATGAAACCGAGAGAGACCTGCCCGAAAAAACTGACCGCTGCATTTACACTCTTTTCCATCTCCTCCGATATTTCCCATTTCTCTCTCTCTCTCTCTCTAAAATTTAGACATATTACTGCAATGGCTTCTCCGCCGACGCCGCCATTGCCGGACGATCTTGCCATTGGCTACTTTTCATCATCTCCTCCTCCTCCTCCTTCGCCGGTCTCCAGTCACACCTCGTTGAAGAATCTCAGTCCCAGCATCTTAATCATTCTTACGATTCTTGCATTTACAGCCTTTTCTTCGGGGATTCTCTGCCTTGTTCTCCGTTACCTTAACCGACGGTGTCTTCTCCGTCTCTCTGCCTTGTCTGGATCGTCATCTTCCTCTACTGTTTCTAGTCGCCGCATTATTCCAGCAGAACAAAGTGCGGTTCGTTGTACTAACTCTTTCTCTCCAATCGAATCGCTTCCTCTGTTCTCTTTCTCCTCCGTCACGCGTCGTTCTTCCACTGCCGCCGCTGATTGCGCCGTTTGTTTGTCGAAATTTGAAGCGGAGGATCAGCTTCGTCTTCTTCCTCTCTGTTGCCATGCGTTTCACGCTCAATGCGTCGATACTTGGCTTCAATCGAACCAGAGTTGTCCTCTTTGCCGCTCCGCCATTTTCGCTTCTGAATCCGATGTAATGAAGGCATCCATGGCTTCCTATGCCGCCGAAGGTCGCGGCGGGGACAGTTTTAGGCTCGAGATTGGCAGTATTAGCCGTAGACAAGCGCCGTCCGACTCTGCCGAAGGAAGGAGATCGTATTCGATAGGATCGTTCGAATATTTCGTCGAAGAAGATTCCGAGGTAAACTTCACCAATGCTCACCGGAGGAGCGTCTCCGATAAGGAGGACATCGAAGCTCCTATATCAGCGGTATCTACAGAGCGAAGCCTAGCGGCGGAAGTCGGGTCTGGAAGGAATTGGCTGAAGGACTACGTCGATAGGCTCTCGAATTCTGTATCATCTCGTGCACTGTCTTTCCGAGGCTCCGGTAGGTTCTTCACCGGAAGTAGTCGACGGAGTGAGGTGACAGTCGCGGGAGAATGGGAACAAGAGAACAACCGCGTCGGAGAGGAAATAAGCGAGTTATTCCGGTGGTTTTCAGGGGTATGA
BLAST of CSPI03G10300 vs. Swiss-Prot
Match: ATL4_ARATH (E3 ubiquitin-protein ligase ATL4 OS=Arabidopsis thaliana GN=ATL4 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.6e-76
Identity = 177/321 (55.14%), Postives = 218/321 (67.91%), Query Frame = 1

Query: 96  SSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGSSSSSTVSSR 155
           S  +SL +L PS+L+I+ IL  T   S  +C +LR LNR     +  LS SSS +TV+S 
Sbjct: 16  SHSSSLDSLKPSVLVIILILLMTLLISVSICFLLRCLNRCSHRSVLPLSSSSSVATVTSD 75

Query: 156 RIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTA-AADCAVCLSKFEAEDQLRLLPL 215
               +       T   S ++SLP+F FSSVTRRSS+  + DCAVCLSKFE EDQLRLLPL
Sbjct: 76  SRRFSGHRVSPETERSSVLDSLPIFKFSSVTRRSSSMNSGDCAVCLSKFEPEDQLRLLPL 135

Query: 216 CCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKASMASYAAEGRGGDSFRLEIGSIS 275
           CCHAFHA C+D WL SNQ+CPLCRS +FASESD+MK+     +  G G +SFRLEIGSIS
Sbjct: 136 CCHAFHADCIDIWLVSNQTCPLCRSPLFASESDLMKSLAVVGSNNGGGENSFRLEIGSIS 195

Query: 276 -RRQAP-SDSAEGRRSYSIGSFEYFVEE-DSEVNFTNAHRRSVSDKEDIEAPISAVST-- 335
            RRQ P  +S E  R+YSIGSF+Y V++ DSE++ +N +R    D     A  +AV+T  
Sbjct: 196 RRRQTPIPESVEQHRTYSIGSFDYIVDDVDSEISESNFNRGKQEDATTTTATATAVTTNP 255

Query: 336 ---ERSLAAEVGS--GRNWLKDYVDRLSNSVSSRALSFRGSGRFFTGSSRRSEVTVAGEW 395
              E SLAA++G+   R+WLKDYVDRLS  +SSRA+SFR SGRFFTGSSRRSE       
Sbjct: 256 TSFEASLAADIGNDGSRSWLKDYVDRLSRGISSRAMSFRSSGRFFTGSSRRSEELTV--M 315

Query: 396 EQENNRVGEEISELFRWFSGV 406
           + E N  GEEISELFRW SGV
Sbjct: 316 DLEANHAGEEISELFRWLSGV 334

BLAST of CSPI03G10300 vs. Swiss-Prot
Match: ATL49_ARATH (Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana GN=ATL49 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.2e-19
Identity = 95/317 (29.97%), Postives = 146/317 (46.06%), Query Frame = 1

Query: 85  SSSPPPPPSPVSSHTSLKNLS----PSILIILTILAFTAFSSGILCLVLRYLNRRCLLRL 144
           SSSPPPP  P+ S+TSL NL+    P+IL+I+ IL+   F SG+L +++++L        
Sbjct: 16  SSSPPPPLIPLKSNTSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFLLTPSRESR 75

Query: 145 SALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCL 204
                + ++     +++     S V      S I++LP+F + S+      +  DC VCL
Sbjct: 76  EDYFDNVTALQGQLQQLFNLHDSGV----DQSLIDTLPVFHYKSIVGLK-ISPFDCPVCL 135

Query: 205 SKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKASMASYAAEG 264
            +FE ED+LRLLP C HAFH +C+DTWL S+ +CPLCRS + +  S     S +SY    
Sbjct: 136 CEFETEDKLRLLPKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLS-SSYL--- 195

Query: 265 RGGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNFTNAHRRSVSDKEDI 324
                  LE    SR   P   +  +  Y        V  DSE     + R+S     D+
Sbjct: 196 -----LVLESEQSSRDMVPVLESNSQLGYD-------VNNDSESTRIRSGRKSCDPDGDM 255

Query: 325 E------APISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSGRFFTGSSR 384
           +       P+     +      VG G +  K+ +   S +V  R     GS  +      
Sbjct: 256 DGLDEKVVPLEVKLGKFRNIDHVGEGSDQKKNSISGNSKNVDGRRCLSMGSYEYIMDQEA 311

Query: 385 RSEVTVAGEWEQENNRV 392
             +V V+ +     +RV
Sbjct: 316 TLKVHVSTKKLSGKDRV 311

BLAST of CSPI03G10300 vs. Swiss-Prot
Match: ATL46_ARATH (RING-H2 finger protein ATL46 OS=Arabidopsis thaliana GN=ATL46 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.9e-17
Identity = 90/328 (27.44%), Postives = 145/328 (44.21%), Query Frame = 1

Query: 87  SPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGS 146
           SPP PP P S +     +SP++L ++ ILA   F SG+L L++R+L +      +A S S
Sbjct: 28  SPPSPPPPSSGN----RISPAVLFVIVILAVLFFISGLLHLLVRFLIKHP--SATASSRS 87

Query: 147 SSSSTVSSRRIIPAEQSAVRCTNSFSP----IESLPLFSFSSVTRRSSTAAA-------- 206
           +    +S+   +  +   +   N        I++LP+F +  +   +             
Sbjct: 88  NRFPEISTSDALQRQLQQLFHLNDSGLDQAFIDALPVFHYKEIVGSAGGGGGNGAAQEPF 147

Query: 207 DCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFA----------- 266
           DCAVCL +F  +D+LRLLP+C HAFH  C+DTWLQSN +CPLCR  +F+           
Sbjct: 148 DCAVCLCEFSEKDKLRLLPMCSHAFHLNCIDTWLQSNSTCPLCRGTLFSPGFSMENPMFD 207

Query: 267 -----------SESDVMKASMASYAAEGRGGDSFRL-------EIGS------ISRRQAP 326
                      +E+   K          +G    RL        +G+      ++  +  
Sbjct: 208 FDDIREDEEGVTENGSQKTMEIQEIVVEKGVLPVRLGKFKRLDNVGNGQGQDVVAGGETS 267

Query: 327 SDSAEGRRSYSIGSFEYFV-EEDSEVNFTNAH--RRSVSDKEDIEAPISAVSTERSLAAE 359
           S + + RR +S+GS++Y +   + +V F N    R    DKE  E   ++ S +      
Sbjct: 268 SSNLDARRCFSMGSYQYILGNSELKVPFANDRLPRLKPQDKES-EQTGNSSSEDNKKINT 327

BLAST of CSPI03G10300 vs. Swiss-Prot
Match: ATL13_ARATH (RING-H2 finger protein ATL13 OS=Arabidopsis thaliana GN=ATL13 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.7e-16
Identity = 63/165 (38.18%), Postives = 93/165 (56.36%), Query Frame = 1

Query: 85  SSSPPPPPSPVSSHTSL---KNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLS 144
           SS P  PP  + S  +      +SPSIL+I+ IL+   F SG+L L++R+L         
Sbjct: 25  SSLPQEPPLSLRSSANFDLNSKISPSILLIIIILSIIFFISGLLHLLVRFLLTPSSRDRE 84

Query: 145 ALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCLS 204
               + ++     +++     S V    SF  I++LP+F + S+    +    DCAVCL 
Sbjct: 85  DYFDNVTALQGQLQQLFHLHDSGV--DQSF--IDTLPVFHYKSIIGLKNYPF-DCAVCLC 144

Query: 205 KFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASES 247
           +FE ED+LRLLP C HAFH  C+DTWL S+ +CPLCRS++ +  S
Sbjct: 145 EFETEDKLRLLPKCSHAFHMDCIDTWLLSHSTCPLCRSSLLSDLS 184

BLAST of CSPI03G10300 vs. Swiss-Prot
Match: ATL50_ARATH (Putative RING-H2 finger protein ATL50 OS=Arabidopsis thaliana GN=ATL50 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.9e-15
Identity = 52/136 (38.24%), Postives = 80/136 (58.82%), Query Frame = 1

Query: 104 LSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGSSSSST-VSSRRIIPAEQ 163
           +SP +L+ +T+L+   F + ++ L++++L+R       A  G + SST +  R       
Sbjct: 32  ISPIVLLYITLLSIIFFVAALIHLLVKFLHRPQTRLDDAYDGITESSTALQGRYQTRFNL 91

Query: 164 SAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQ 223
                  SF  I++LPL  + ++       + DCAVCL +F AED+LRLLP C HAFH +
Sbjct: 92  HDAEIDQSF--IDALPLLHYKTMIGLRHDLS-DCAVCLREFTAEDELRLLPKCSHAFHVE 151

Query: 224 CVDTWLQSNQSCPLCR 239
           C+DTWL +N +CPLCR
Sbjct: 152 CIDTWLLTNSTCPLCR 164

BLAST of CSPI03G10300 vs. TrEMBL
Match: A0A0A0L3Z4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G128900 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 3.5e-181
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 1

Query: 67  MASPPTPPLPDDLAIGYFSSSPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILC 126
           MASPPTPPLPDDLAIGYFSSSPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILC
Sbjct: 1   MASPPTPPLPDDLAIGYFSSSPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILC 60

Query: 127 LVLRYLNRRCLLRLSALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVT 186
           LVLRYLNRRCLLRLSALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVT
Sbjct: 61  LVLRYLNRRCLLRLSALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVT 120

Query: 187 RRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASES 246
           RRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASES
Sbjct: 121 RRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASES 180

Query: 247 DVMKASMASYAAEGRGGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNF 306
           DVMKASMASYAAEGRGGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNF
Sbjct: 181 DVMKASMASYAAEGRGGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNF 240

Query: 307 TNAHRRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSG 366
           TNAHRRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSG
Sbjct: 241 TNAHRRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSG 300

Query: 367 RFFTGSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV 406
           RFFTGSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV
Sbjct: 301 RFFTGSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV 339

BLAST of CSPI03G10300 vs. TrEMBL
Match: A0A067JYE0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14673 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.8e-97
Identity = 211/335 (62.99%), Postives = 255/335 (76.12%), Query Frame = 1

Query: 85  SSSPPPPPSPV--------SSHTS-LKNLSPSILIILTILAFTAFSSGILCLVLRYLNRR 144
           SSS P PPSP+         +HTS  +NL PSIL+I+ IL+ T   S  LCL+LR+LNRR
Sbjct: 35  SSSSPSPPSPLLPFNNNSEDAHTSPFQNLKPSILVIVLILSITFLLSISLCLLLRHLNRR 94

Query: 145 CLLRLSALSGSSSSS-TVSSRRIIPAEQSAVRCTN---SFSPIESLPLFSFSSVTRRSST 204
           CL  LS  S +S+++ T+++        S  R +    +FS I SLPLFSFSS+ RRS+T
Sbjct: 95  CLRHLSPTSHASTTAITITTSSASSTRHSTNRVSPESPAFSIINSLPLFSFSSIKRRSAT 154

Query: 205 AAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKA 264
            + DCAVCLSKFE++DQLRLLPLCCHAFHA C+DTWLQSNQ+CPLCRS I ASESD+ KA
Sbjct: 155 TSGDCAVCLSKFESQDQLRLLPLCCHAFHAHCIDTWLQSNQTCPLCRSPIHASESDLFKA 214

Query: 265 SMASYAAEGRGGDSFRLEIGSISRRQAPSDSA-EGRRSYSIGSFEYFVEEDSEVNFTNAH 324
             +S   +G G +SFRLEIGSISRRQA SDSA E R SYS+GSFEY VEE+SEV  ++ H
Sbjct: 215 LSSSI--DGSGAESFRLEIGSISRRQAASDSAVETRGSYSVGSFEYVVEEESEVTMSHTH 274

Query: 325 RRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSGRFFT 384
           RRSVSDKE I AP+S    E +LA+EV SGR+WLKDYVDRLSN++SSRALSFR SGRFFT
Sbjct: 275 RRSVSDKE-IVAPVSPSCQEPNLASEVASGRSWLKDYVDRLSNTLSSRALSFRSSGRFFT 334

Query: 385 GSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV 406
           GSSRRSE+   G+++ E NR+GEEISE+FRWFSGV
Sbjct: 335 GSSRRSEIGSVGDYDVEANRMGEEISEMFRWFSGV 366

BLAST of CSPI03G10300 vs. TrEMBL
Match: A0A061DVX8_THECC (Toxicos en levadura 4, putative OS=Theobroma cacao GN=TCM_005794 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 5.5e-94
Identity = 201/323 (62.23%), Postives = 248/323 (76.78%), Query Frame = 1

Query: 93  SPVSSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGSS--SSS 152
           S  SS +S++N+ PSI+II+ IL+ T   S  LCL+LR+LNRRCL  LS  + S+  S++
Sbjct: 36  SSSSSSSSIENVKPSIIIIILILSITVLVSVSLCLLLRHLNRRCLRHLSRSTTSTITSAA 95

Query: 153 TVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRS---STAAADCAVCLSKFEAED 212
             +S R+ P E          S ++SLP+F+FSS+ RRS   ST + DCAVCLSKFE+ D
Sbjct: 96  ATASHRVSPEESQTA------SLLDSLPVFTFSSIIRRSNNDSTVSGDCAVCLSKFESHD 155

Query: 213 QLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKA---SMASYAAEGRGG- 272
           QLRLLPLCCHAFHA C+DTWL SNQ+CPLCRS +FASESD+MK    S  S AA G GG 
Sbjct: 156 QLRLLPLCCHAFHAHCIDTWLASNQTCPLCRSPLFASESDLMKVLLQSSNSAAAIGSGGS 215

Query: 273 DSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNFTNAHRRSVSDKEDIEAP 332
           DSFRLEIGSISRRQ  S+S E RRSYSIGSF+Y VEE+SEV     HRR+VSDKED+ AP
Sbjct: 216 DSFRLEIGSISRRQPGSESGEQRRSYSIGSFDYVVEEESEVTRNQTHRRTVSDKEDVAAP 275

Query: 333 ISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSGRFFTGSSRRSEVT-VAG 392
           ++A  +E SLAAEV +GR+WLK+YVDRLS S+SSRA+SFR SGRFFTGSSRRS+++ VA 
Sbjct: 276 VAA--SEASLAAEVATGRSWLKEYVDRLSFSLSSRAMSFRSSGRFFTGSSRRSDISGVAV 335

Query: 393 EWEQENNRVGEEISELFRWFSGV 406
           +++ E NR+GEEISE+FRWFSGV
Sbjct: 336 DYDVEANRIGEEISEMFRWFSGV 350

BLAST of CSPI03G10300 vs. TrEMBL
Match: A0A0D2R0B8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G067900 PE=4 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.4e-89
Identity = 202/349 (57.88%), Postives = 251/349 (71.92%), Query Frame = 1

Query: 67  MASPPTPPLPDDLAIGYFSSSPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILC 126
           MASPP  PL   +  G  +SS         S +S+++  PSI+II+ IL+ T   S  LC
Sbjct: 1   MASPP--PLLYGVIGGPVASSENNSSERSHSSSSIESAKPSIIIIILILSITLLVSVSLC 60

Query: 127 LVLRYLNRRCLLRLSALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVT 186
           L+LR+LNRRCL  LS  S S+  ++ +S R+ P EQS          ++SLPLF+FSS+T
Sbjct: 61  LLLRHLNRRCLRHLSRSSTSTIVASAASHRVSP-EQSPTALL-----LDSLPLFTFSSIT 120

Query: 187 RR----SSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIF 246
           RR     ST + DCAVCLSKFE +DQLRLLPLCCHAFHAQC+DTWL SNQ+CPLCRS +F
Sbjct: 121 RRRSNGDSTVSGDCAVCLSKFEQQDQLRLLPLCCHAFHAQCIDTWLTSNQTCPLCRSPLF 180

Query: 247 ASESDVMKASMASYAAE---GRGG-DSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFV 306
           ASESD+MK+ + S  A    G GG DSFRLEIGS+S RQ  S+S E RRSYSIGSF+Y V
Sbjct: 181 ASESDLMKSLLQSSNAAAPIGSGGSDSFRLEIGSVSLRQPGSESGEQRRSYSIGSFDYIV 240

Query: 307 EEDSEVNFTNAHRRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSR 366
           EE+SEV     H+R+VSDKE++     A S E SLA EV +GR+WLK+YVDRLS+S+SSR
Sbjct: 241 EEESEVTRNQTHQRNVSDKEEVVGGAEAAS-EASLAGEVATGRSWLKEYVDRLSSSLSSR 300

Query: 367 ALSFRGSGRFFTGSSRRSEV--TVAGEWEQENNRVGEEISELFRWFSGV 406
           A+SFR SGRFFTGSSRRS++   V  +++ E NR+GEEISE+FRWFSGV
Sbjct: 301 AMSFRSSGRFFTGSSRRSDIAGVVTADYDVEANRIGEEISEMFRWFSGV 340

BLAST of CSPI03G10300 vs. TrEMBL
Match: W9R595_9ROSA (E3 ubiquitin-protein ligase ATL4 OS=Morus notabilis GN=L484_008028 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.1e-89
Identity = 210/362 (58.01%), Postives = 251/362 (69.34%), Query Frame = 1

Query: 85  SSSPPPPPS-------------PVSSHT--SLKNLSPSILIILTILAFTAFSSGILCLVL 144
           SSSPPP PS             P  +HT  SL N+SPSI II+ ILA T  +S  LC +L
Sbjct: 2   SSSPPPWPSVFGASATPMSFGTPPPTHTVSSLGNISPSIFIIIIILAVTVIASVSLCFLL 61

Query: 145 RYLNRRCLLRLSALSGSSSSST-------------VSSRRIIPAEQSAVRCTNSFSPIES 204
           R+LNRRCL  LS LS SSS S+             +SSRR+ PAE +     ++ S IE+
Sbjct: 62  RHLNRRCLRHLSPLSSSSSPSSSTAAAAVGDSHRAISSRRVSPAENN-----HNSSLIEA 121

Query: 205 LPLFSFSSVTRR----SSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQ 264
           LPLF+F+SVTRR    S+  AADCAVCLSKFE +DQLRLLPLCCHAFHA CVDTWLQSNQ
Sbjct: 122 LPLFAFASVTRRGAATSAIFAADCAVCLSKFEPDDQLRLLPLCCHAFHALCVDTWLQSNQ 181

Query: 265 SCPLCRSAIFASESDVMKASMASYAAEGRGGDSFRLEIGSISRRQ-APSDS------AEG 324
           +CPLCRS IF S+++++K S+      G    SFRLEIG++SRRQ  PSDS         
Sbjct: 182 TCPLCRSPIFVSDAEILK-SLGEINRRGDNSGSFRLEIGNVSRRQTTPSDSNFSGDLGRR 241

Query: 325 RRSYSIGSFEYFVEEDSEVNFTNAHRRSVSDKEDIEAPISAVSTERSLAAEV-GSGRNWL 384
            RSYSIGSF+Y VEEDSEV  TNAHRR++SD ++   P S    E S+ AEV  SGR+WL
Sbjct: 242 SRSYSIGSFDYLVEEDSEVVLTNAHRRTISDFKEPPPPASIPVPEPSVVAEVASSGRSWL 301

Query: 385 KDYVDRLSNSVSSRALSFRGSGRFFTGSSRRSEVTV-AGEWEQENNRVGEEISELFRWFS 406
           K+YVDRLS S+SSRA+SFR SGRFF+GSSRRS+V +  GEW+ E +RVGEEISELFRW S
Sbjct: 302 KEYVDRLSMSLSSRAMSFRSSGRFFSGSSRRSDVVLGVGEWDLEASRVGEEISELFRWVS 357

BLAST of CSPI03G10300 vs. TAIR10
Match: AT3G60220.1 (AT3G60220.1 TOXICOS EN LEVADURA 4)

HSP 1 Score: 286.2 bits (731), Expect = 3.2e-77
Identity = 177/321 (55.14%), Postives = 218/321 (67.91%), Query Frame = 1

Query: 96  SSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGSSSSSTVSSR 155
           S  +SL +L PS+L+I+ IL  T   S  +C +LR LNR     +  LS SSS +TV+S 
Sbjct: 16  SHSSSLDSLKPSVLVIILILLMTLLISVSICFLLRCLNRCSHRSVLPLSSSSSVATVTSD 75

Query: 156 RIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTA-AADCAVCLSKFEAEDQLRLLPL 215
               +       T   S ++SLP+F FSSVTRRSS+  + DCAVCLSKFE EDQLRLLPL
Sbjct: 76  SRRFSGHRVSPETERSSVLDSLPIFKFSSVTRRSSSMNSGDCAVCLSKFEPEDQLRLLPL 135

Query: 216 CCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKASMASYAAEGRGGDSFRLEIGSIS 275
           CCHAFHA C+D WL SNQ+CPLCRS +FASESD+MK+     +  G G +SFRLEIGSIS
Sbjct: 136 CCHAFHADCIDIWLVSNQTCPLCRSPLFASESDLMKSLAVVGSNNGGGENSFRLEIGSIS 195

Query: 276 -RRQAP-SDSAEGRRSYSIGSFEYFVEE-DSEVNFTNAHRRSVSDKEDIEAPISAVST-- 335
            RRQ P  +S E  R+YSIGSF+Y V++ DSE++ +N +R    D     A  +AV+T  
Sbjct: 196 RRRQTPIPESVEQHRTYSIGSFDYIVDDVDSEISESNFNRGKQEDATTTTATATAVTTNP 255

Query: 336 ---ERSLAAEVGS--GRNWLKDYVDRLSNSVSSRALSFRGSGRFFTGSSRRSEVTVAGEW 395
              E SLAA++G+   R+WLKDYVDRLS  +SSRA+SFR SGRFFTGSSRRSE       
Sbjct: 256 TSFEASLAADIGNDGSRSWLKDYVDRLSRGISSRAMSFRSSGRFFTGSSRRSEELTV--M 315

Query: 396 EQENNRVGEEISELFRWFSGV 406
           + E N  GEEISELFRW SGV
Sbjct: 316 DLEANHAGEEISELFRWLSGV 334

BLAST of CSPI03G10300 vs. TAIR10
Match: AT2G18650.1 (AT2G18650.1 RING/U-box superfamily protein)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-20
Identity = 95/317 (29.97%), Postives = 146/317 (46.06%), Query Frame = 1

Query: 85  SSSPPPPPSPVSSHTSLKNLS----PSILIILTILAFTAFSSGILCLVLRYLNRRCLLRL 144
           SSSPPPP  P+ S+TSL NL+    P+IL+I+ IL+   F SG+L +++++L        
Sbjct: 16  SSSPPPPLIPLKSNTSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFLLTPSRESR 75

Query: 145 SALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCL 204
                + ++     +++     S V      S I++LP+F + S+      +  DC VCL
Sbjct: 76  EDYFDNVTALQGQLQQLFNLHDSGV----DQSLIDTLPVFHYKSIVGLK-ISPFDCPVCL 135

Query: 205 SKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKASMASYAAEG 264
            +FE ED+LRLLP C HAFH +C+DTWL S+ +CPLCRS + +  S     S +SY    
Sbjct: 136 CEFETEDKLRLLPKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLS-SSYL--- 195

Query: 265 RGGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNFTNAHRRSVSDKEDI 324
                  LE    SR   P   +  +  Y        V  DSE     + R+S     D+
Sbjct: 196 -----LVLESEQSSRDMVPVLESNSQLGYD-------VNNDSESTRIRSGRKSCDPDGDM 255

Query: 325 E------APISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSGRFFTGSSR 384
           +       P+     +      VG G +  K+ +   S +V  R     GS  +      
Sbjct: 256 DGLDEKVVPLEVKLGKFRNIDHVGEGSDQKKNSISGNSKNVDGRRCLSMGSYEYIMDQEA 311

Query: 385 RSEVTVAGEWEQENNRV 392
             +V V+ +     +RV
Sbjct: 316 TLKVHVSTKKLSGKDRV 311

BLAST of CSPI03G10300 vs. TAIR10
Match: AT5G40250.1 (AT5G40250.1 RING/U-box superfamily protein)

HSP 1 Score: 90.1 bits (222), Expect = 3.3e-18
Identity = 90/328 (27.44%), Postives = 145/328 (44.21%), Query Frame = 1

Query: 87  SPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGS 146
           SPP PP P S +     +SP++L ++ ILA   F SG+L L++R+L +      +A S S
Sbjct: 28  SPPSPPPPSSGN----RISPAVLFVIVILAVLFFISGLLHLLVRFLIKHP--SATASSRS 87

Query: 147 SSSSTVSSRRIIPAEQSAVRCTNSFSP----IESLPLFSFSSVTRRSSTAAA-------- 206
           +    +S+   +  +   +   N        I++LP+F +  +   +             
Sbjct: 88  NRFPEISTSDALQRQLQQLFHLNDSGLDQAFIDALPVFHYKEIVGSAGGGGGNGAAQEPF 147

Query: 207 DCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFA----------- 266
           DCAVCL +F  +D+LRLLP+C HAFH  C+DTWLQSN +CPLCR  +F+           
Sbjct: 148 DCAVCLCEFSEKDKLRLLPMCSHAFHLNCIDTWLQSNSTCPLCRGTLFSPGFSMENPMFD 207

Query: 267 -----------SESDVMKASMASYAAEGRGGDSFRL-------EIGS------ISRRQAP 326
                      +E+   K          +G    RL        +G+      ++  +  
Sbjct: 208 FDDIREDEEGVTENGSQKTMEIQEIVVEKGVLPVRLGKFKRLDNVGNGQGQDVVAGGETS 267

Query: 327 SDSAEGRRSYSIGSFEYFV-EEDSEVNFTNAH--RRSVSDKEDIEAPISAVSTERSLAAE 359
           S + + RR +S+GS++Y +   + +V F N    R    DKE  E   ++ S +      
Sbjct: 268 SSNLDARRCFSMGSYQYILGNSELKVPFANDRLPRLKPQDKES-EQTGNSSSEDNKKINT 327

BLAST of CSPI03G10300 vs. TAIR10
Match: AT4G30400.1 (AT4G30400.1 RING/U-box superfamily protein)

HSP 1 Score: 88.6 bits (218), Expect = 9.7e-18
Identity = 63/165 (38.18%), Postives = 93/165 (56.36%), Query Frame = 1

Query: 85  SSSPPPPPSPVSSHTSL---KNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLS 144
           SS P  PP  + S  +      +SPSIL+I+ IL+   F SG+L L++R+L         
Sbjct: 25  SSLPQEPPLSLRSSANFDLNSKISPSILLIIIILSIIFFISGLLHLLVRFLLTPSSRDRE 84

Query: 145 ALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCLS 204
               + ++     +++     S V    SF  I++LP+F + S+    +    DCAVCL 
Sbjct: 85  DYFDNVTALQGQLQQLFHLHDSGV--DQSF--IDTLPVFHYKSIIGLKNYPF-DCAVCLC 144

Query: 205 KFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASES 247
           +FE ED+LRLLP C HAFH  C+DTWL S+ +CPLCRS++ +  S
Sbjct: 145 EFETEDKLRLLPKCSHAFHMDCIDTWLLSHSTCPLCRSSLLSDLS 184

BLAST of CSPI03G10300 vs. TAIR10
Match: AT5G57750.1 (AT5G57750.1 RING/U-box superfamily protein)

HSP 1 Score: 85.1 bits (209), Expect = 1.1e-16
Identity = 52/136 (38.24%), Postives = 80/136 (58.82%), Query Frame = 1

Query: 104 LSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGSSSSST-VSSRRIIPAEQ 163
           +SP +L+ +T+L+   F + ++ L++++L+R       A  G + SST +  R       
Sbjct: 32  ISPIVLLYITLLSIIFFVAALIHLLVKFLHRPQTRLDDAYDGITESSTALQGRYQTRFNL 91

Query: 164 SAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQ 223
                  SF  I++LPL  + ++       + DCAVCL +F AED+LRLLP C HAFH +
Sbjct: 92  HDAEIDQSF--IDALPLLHYKTMIGLRHDLS-DCAVCLREFTAEDELRLLPKCSHAFHVE 151

Query: 224 CVDTWLQSNQSCPLCR 239
           C+DTWL +N +CPLCR
Sbjct: 152 CIDTWLLTNSTCPLCR 164

BLAST of CSPI03G10300 vs. NCBI nr
Match: gi|778677680|ref|XP_004134365.2| (PREDICTED: E3 ubiquitin-protein ligase ATL4 [Cucumis sativus])

HSP 1 Score: 718.4 bits (1853), Expect = 7.1e-204
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 1

Query: 27  MKPRETCPKKLTAAFTLFSISSDISHFSLSLSLKFRHITAMASPPTPPLPDDLAIGYFSS 86
           MKPRETCPKKLTAAFTLFSISSDISHFSLSLSLKFRHITAMASPPTPPLPDDLAIGYFSS
Sbjct: 1   MKPRETCPKKLTAAFTLFSISSDISHFSLSLSLKFRHITAMASPPTPPLPDDLAIGYFSS 60

Query: 87  SPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGS 146
           SPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGS
Sbjct: 61  SPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLSALSGS 120

Query: 147 SSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCLSKFEAE 206
           SSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCLSKFEAE
Sbjct: 121 SSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSSTAAADCAVCLSKFEAE 180

Query: 207 DQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKASMASYAAEGRGGDSF 266
           DQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKASMASYAAEGRGGDSF
Sbjct: 181 DQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKASMASYAAEGRGGDSF 240

Query: 267 RLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNFTNAHRRSVSDKEDIEAPISA 326
           RLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNFTNAHRRSVSDKEDIEAPISA
Sbjct: 241 RLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNFTNAHRRSVSDKEDIEAPISA 300

Query: 327 VSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSGRFFTGSSRRSEVTVAGEWEQ 386
           VSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSGRFFTGSSRRSEVTVAGEWEQ
Sbjct: 301 VSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSGRFFTGSSRRSEVTVAGEWEQ 360

Query: 387 ENNRVGEEISELFRWFSGV 406
           ENNRVGEEISELFRWFSGV
Sbjct: 361 ENNRVGEEISELFRWFSGV 379

BLAST of CSPI03G10300 vs. NCBI nr
Match: gi|700201547|gb|KGN56680.1| (hypothetical protein Csa_3G128900 [Cucumis sativus])

HSP 1 Score: 642.5 bits (1656), Expect = 5.0e-181
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 1

Query: 67  MASPPTPPLPDDLAIGYFSSSPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILC 126
           MASPPTPPLPDDLAIGYFSSSPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILC
Sbjct: 1   MASPPTPPLPDDLAIGYFSSSPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILC 60

Query: 127 LVLRYLNRRCLLRLSALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVT 186
           LVLRYLNRRCLLRLSALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVT
Sbjct: 61  LVLRYLNRRCLLRLSALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVT 120

Query: 187 RRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASES 246
           RRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASES
Sbjct: 121 RRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASES 180

Query: 247 DVMKASMASYAAEGRGGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNF 306
           DVMKASMASYAAEGRGGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNF
Sbjct: 181 DVMKASMASYAAEGRGGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEEDSEVNF 240

Query: 307 TNAHRRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSG 366
           TNAHRRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSG
Sbjct: 241 TNAHRRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSG 300

Query: 367 RFFTGSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV 406
           RFFTGSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV
Sbjct: 301 RFFTGSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV 339

BLAST of CSPI03G10300 vs. NCBI nr
Match: gi|659075688|ref|XP_008438276.1| (PREDICTED: E3 ubiquitin-protein ligase ATL4 [Cucumis melo])

HSP 1 Score: 592.0 bits (1525), Expect = 7.7e-166
Identity = 322/345 (93.33%), Postives = 330/345 (95.65%), Query Frame = 1

Query: 67  MASPPTPPLPDDL---AIGYFSSSPPPPPSPVSSH--TSLKNLSPSILIILTILAFTAFS 126
           MASPP PPLPDD    AI Y SSSPP P SPVSS   +SLKNLSPSILIILTILAFTAF+
Sbjct: 1   MASPPPPPLPDDFSGSAIDYVSSSPPLPQSPVSSSHSSSLKNLSPSILIILTILAFTAFA 60

Query: 127 SGILCLVLRYLNRRCLLRLSALSGSSSSSTVSSRRIIPAEQSAVRCTNSFSPIESLPLFS 186
           S ILCLVLRYLNRRCLLRLSA+SGSSSSS+VSSRRIIPAEQ+AVRCTNSFSPIESLPLFS
Sbjct: 61  SVILCLVLRYLNRRCLLRLSAVSGSSSSSSVSSRRIIPAEQTAVRCTNSFSPIESLPLFS 120

Query: 187 FSSVTRRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAI 246
           FSSVTRRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAI
Sbjct: 121 FSSVTRRSSTAAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAI 180

Query: 247 FASESDVMKASMASYAAEGR-GGDSFRLEIGSISRRQAPSDSAEGRRSYSIGSFEYFVEE 306
           FASESDVMKASMASYAAEGR GGDSFRLEIGSISRRQAPSDS EGRRSYSIGSFEYFVEE
Sbjct: 181 FASESDVMKASMASYAAEGRGGGDSFRLEIGSISRRQAPSDSGEGRRSYSIGSFEYFVEE 240

Query: 307 DSEVNFTNAHRRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRAL 366
           DSEVNFTNAHRRSVSDKEDIEAPISAVST+RSLAA+VGSGRNWLKDYVDRLSNSVSSRAL
Sbjct: 241 DSEVNFTNAHRRSVSDKEDIEAPISAVSTDRSLAADVGSGRNWLKDYVDRLSNSVSSRAL 300

Query: 367 SFRGSGRFFTGSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV 406
           SFRGSGRFFTGSSRRSEVTVAGEWEQEN+RVGEEISELFRWFSGV
Sbjct: 301 SFRGSGRFFTGSSRRSEVTVAGEWEQENSRVGEEISELFRWFSGV 345

BLAST of CSPI03G10300 vs. NCBI nr
Match: gi|1009170531|ref|XP_015866250.1| (PREDICTED: E3 ubiquitin-protein ligase ATL4 [Ziziphus jujuba])

HSP 1 Score: 365.5 bits (937), Expect = 1.2e-97
Identity = 222/347 (63.98%), Postives = 258/347 (74.35%), Query Frame = 1

Query: 83  YFSSSPPPPPSPVSSHTSLKNLSPSILIILTILAFTAFSSGILCLVLRYLNRRCLLRLS- 142
           Y++S+   PP   S+ TS  NLSPSILII+ ILA T  +S  LCL+LR+LNRRCL  LS 
Sbjct: 26  YYNSTASSPPHHHSTSTSFSNLSPSILIIVLILAITFIASVSLCLLLRHLNRRCLRHLSQ 85

Query: 143 --ALSGSSSSSTV--------SSRRIIPAEQSAVRCTNSFSPIESLPLFSFSSVTRRSST 202
             + S SSSSSTV        S+RR+ P + +A       S I+SLPLF+FSSVTRRSS+
Sbjct: 86  SASSSFSSSSSTVIPLESHRISARRVSPEDPTA-------SLIDSLPLFTFSSVTRRSSS 145

Query: 203 ----AAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESD 262
               AAADCAVCLSKFE +DQLRLLPLCCHAFHA CVDTWLQSNQ+CPLCRS IFASE++
Sbjct: 146 SGAAAAADCAVCLSKFEPDDQLRLLPLCCHAFHALCVDTWLQSNQTCPLCRSPIFASETE 205

Query: 263 VMKASMASYAAE---GRGGDSFRLEIGSISRRQAPSDSAEG----RRSYSIGSFEYFVEE 322
           ++K+  A+   E   GR G SFRLEIGS+S RQ  S    G    RRSYSIGSFEY VE+
Sbjct: 206 ILKSLTAANTGENTSGRSGGSFRLEIGSVSGRQTESIGDGGGRDRRRSYSIGSFEYLVED 265

Query: 323 DSEVNFTNAHRRSVSDKEDIEAPISAVSTERSLAAEV--GSGRNWLKDYVDRLSNSVSSR 382
           DSEV+ +NAHRRSVSD++D   P   V  E SLA EV  GSGR+WLK+YVDRLS S+SSR
Sbjct: 266 DSEVSLSNAHRRSVSDQKDDGQPPGMV-PEESLAGEVAAGSGRSWLKEYVDRLSMSLSSR 325

Query: 383 ALSFRGSGRFFTGSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV 406
           A SFR SGRFFTGSSR+SEVTVAGEW+ E  RVGEEISE+FRWFSGV
Sbjct: 326 AQSFRSSGRFFTGSSRQSEVTVAGEWDMEAARVGEEISEMFRWFSGV 364

BLAST of CSPI03G10300 vs. NCBI nr
Match: gi|643717276|gb|KDP28902.1| (hypothetical protein JCGZ_14673 [Jatropha curcas])

HSP 1 Score: 364.4 bits (934), Expect = 2.6e-97
Identity = 211/335 (62.99%), Postives = 255/335 (76.12%), Query Frame = 1

Query: 85  SSSPPPPPSPV--------SSHTS-LKNLSPSILIILTILAFTAFSSGILCLVLRYLNRR 144
           SSS P PPSP+         +HTS  +NL PSIL+I+ IL+ T   S  LCL+LR+LNRR
Sbjct: 35  SSSSPSPPSPLLPFNNNSEDAHTSPFQNLKPSILVIVLILSITFLLSISLCLLLRHLNRR 94

Query: 145 CLLRLSALSGSSSSS-TVSSRRIIPAEQSAVRCTN---SFSPIESLPLFSFSSVTRRSST 204
           CL  LS  S +S+++ T+++        S  R +    +FS I SLPLFSFSS+ RRS+T
Sbjct: 95  CLRHLSPTSHASTTAITITTSSASSTRHSTNRVSPESPAFSIINSLPLFSFSSIKRRSAT 154

Query: 205 AAADCAVCLSKFEAEDQLRLLPLCCHAFHAQCVDTWLQSNQSCPLCRSAIFASESDVMKA 264
            + DCAVCLSKFE++DQLRLLPLCCHAFHA C+DTWLQSNQ+CPLCRS I ASESD+ KA
Sbjct: 155 TSGDCAVCLSKFESQDQLRLLPLCCHAFHAHCIDTWLQSNQTCPLCRSPIHASESDLFKA 214

Query: 265 SMASYAAEGRGGDSFRLEIGSISRRQAPSDSA-EGRRSYSIGSFEYFVEEDSEVNFTNAH 324
             +S   +G G +SFRLEIGSISRRQA SDSA E R SYS+GSFEY VEE+SEV  ++ H
Sbjct: 215 LSSSI--DGSGAESFRLEIGSISRRQAASDSAVETRGSYSVGSFEYVVEEESEVTMSHTH 274

Query: 325 RRSVSDKEDIEAPISAVSTERSLAAEVGSGRNWLKDYVDRLSNSVSSRALSFRGSGRFFT 384
           RRSVSDKE I AP+S    E +LA+EV SGR+WLKDYVDRLSN++SSRALSFR SGRFFT
Sbjct: 275 RRSVSDKE-IVAPVSPSCQEPNLASEVASGRSWLKDYVDRLSNTLSSRALSFRSSGRFFT 334

Query: 385 GSSRRSEVTVAGEWEQENNRVGEEISELFRWFSGV 406
           GSSRRSE+   G+++ E NR+GEEISE+FRWFSGV
Sbjct: 335 GSSRRSEIGSVGDYDVEANRMGEEISEMFRWFSGV 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ATL4_ARATH5.6e-7655.14E3 ubiquitin-protein ligase ATL4 OS=Arabidopsis thaliana GN=ATL4 PE=1 SV=1[more]
ATL49_ARATH2.2e-1929.97Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana GN=ATL49 PE=3 SV=1[more]
ATL46_ARATH5.9e-1727.44RING-H2 finger protein ATL46 OS=Arabidopsis thaliana GN=ATL46 PE=2 SV=1[more]
ATL13_ARATH1.7e-1638.18RING-H2 finger protein ATL13 OS=Arabidopsis thaliana GN=ATL13 PE=2 SV=2[more]
ATL50_ARATH1.9e-1538.24Putative RING-H2 finger protein ATL50 OS=Arabidopsis thaliana GN=ATL50 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L3Z4_CUCSA3.5e-181100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G128900 PE=4 SV=1[more]
A0A067JYE0_JATCU1.8e-9762.99Uncharacterized protein OS=Jatropha curcas GN=JCGZ_14673 PE=4 SV=1[more]
A0A061DVX8_THECC5.5e-9462.23Toxicos en levadura 4, putative OS=Theobroma cacao GN=TCM_005794 PE=4 SV=1[more]
A0A0D2R0B8_GOSRA2.4e-8957.88Uncharacterized protein OS=Gossypium raimondii GN=B456_004G067900 PE=4 SV=1[more]
W9R595_9ROSA3.1e-8958.01E3 ubiquitin-protein ligase ATL4 OS=Morus notabilis GN=L484_008028 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G60220.13.2e-7755.14 TOXICOS EN LEVADURA 4[more]
AT2G18650.11.2e-2029.97 RING/U-box superfamily protein[more]
AT5G40250.13.3e-1827.44 RING/U-box superfamily protein[more]
AT4G30400.19.7e-1838.18 RING/U-box superfamily protein[more]
AT5G57750.11.1e-1638.24 RING/U-box superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778677680|ref|XP_004134365.2|7.1e-204100.00PREDICTED: E3 ubiquitin-protein ligase ATL4 [Cucumis sativus][more]
gi|700201547|gb|KGN56680.1|5.0e-181100.00hypothetical protein Csa_3G128900 [Cucumis sativus][more]
gi|659075688|ref|XP_008438276.1|7.7e-16693.33PREDICTED: E3 ubiquitin-protein ligase ATL4 [Cucumis melo][more]
gi|1009170531|ref|XP_015866250.1|1.2e-9763.98PREDICTED: E3 ubiquitin-protein ligase ATL4 [Ziziphus jujuba][more]
gi|643717276|gb|KDP28902.1|2.6e-9762.99hypothetical protein JCGZ_14673 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016874 ligase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G10300.1CSPI03G10300.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 195..238
score: 1.3
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 196..237
score: 1.
IPR001841Zinc finger, RING-typePROFILEPS50089ZF_RING_2coord: 196..238
score: 12
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 194..241
score: 1.6
NoneNo IPR availablePANTHERPTHR14155RING FINGER DOMAIN-CONTAININGcoord: 77..264
score: 6.1
NoneNo IPR availablePANTHERPTHR14155:SF129SUBFAMILY NOT NAMEDcoord: 77..264
score: 6.1
NoneNo IPR availableunknownSSF57850RING/U-boxcoord: 175..244
score: 2.99

The following gene(s) are paralogous to this gene:

None