CSPI03G09240 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI03G09240
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionGlyoxylate reductase
LocationChr3 : 7547300 .. 7548807 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGAAGAAACTGAAGGCGATAGCAATAGCAACGACCTTCATCAAGTTTTGGTTTTAGGTTCTCCGTGGGTATTACCCGCTCTCCAATCTCAATTCTCAAACCGATTCCATTTCCTCCTACCATCTCTTTCCGATCTCCCTCTCCTCCAATTCCTTTCTTCTTATGCCCAATCCACACAAGCCTTGCTCATCCCCGGCGGCTGCTTTCTGGTCACCTCCCCCGTCCTCGATTGCCTTCCGGCGCTCAAGCTCCTTGTCACTACTAGCGCCGGTGTTGACCACCTCGACTTGCCGGAATTACGTCGCCGTCAGATCGCTATTGCCTACGTTCCGGACTTGTACTCTGAAGATGTCGCTGATCTTGCGGTTGGGTTGTTGATTGATGTTCTCATGAAGGTTTCGGCGAGAGATCGGTGCTTTAGGCTACGGCTTCCGCCTACTACCAACCCGGAATTTCCGCCTCTCGGATTAAAGGTGCCATGGATTTTCCTATTTTAACTCAAAACTACTTTTTTCGAGGGAATTTGTCATTATTACCAAATATATATAAAGGGAATAGTAAAAAATGATAAATTTAACAGAAAAAAAATTAAGAGTAACCTGATAAAAATGCTATATTTTAATTTATTTTACTATAGTTAAAATTGTTCTGTATAAAAAAAATTAAGAAAACTATAGACTGACCAATTTCTTTTGTTGTTTTCAGATTTTACAAAAAAAAATTAAAAAGAAAAAAAAAATACTTATGCTAACTTGGGAGAAAAAACAAATATATTTTAAAATGAAAATTTGTCAAAAAATAATCAAATATTTAAATGTAAAAAATTTGGTTTTGTCGATTGTAAATAGTTTGTAAAAAACCCTTTTGATTAATGAATTTCATAATTGATTTTTTTTTTATCACTTTTTGCAGTTAAATGGGAAAAGAATTGGGATAGTTGGATTGGGGAAAATAGGGTCTAAAGTTGCCAAAAGGCTGGAGGGATTTGAGTGCAAAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACTACTCCAATGTACATGAACTTGCAAGCAACTGTGATGGCCTCGTACTTTGTTGTGGGTTAACGAAAGAAACCCAACATATGATCAATAGGGAGGTGATGGCTGCATTAGGAAAAGATGGAGTGATAATCAACGTTGGTAGAGGGGCGATAATCGACGAGAAGGCTATGGTTGAATGTTTGATCAAGGGAGAGATTGGGGGAGTTGGGTTAGATGTGTTTGAGAATGAACCTGAAATTCCTGAAGAGCTCTTTAATTTCGATAATGTTGTATTATCACCACATGTTGCTGTTATGACACATGAAACTTTGCAGGGATTGTCTAGGTTGGTGGTGGACAACTTGGAGGCATTGTTCTCAAACAAGCCTTTGGTGTCTCCATTTCCTAATTAGCATAGGCTTAAAATTATGTCCATGAAATTTGAAATATATTTCATTCTGATCCCGCAATCAAAATCGGCAC

mRNA sequence

ATGGGAGAAGAAACTGAAGGCGATAGCAATAGCAACGACCTTCATCAAGTTTTGGTTTTAGGTTCTCCGTGGGTATTACCCGCTCTCCAATCTCAATTCTCAAACCGATTCCATTTCCTCCTACCATCTCTTTCCGATCTCCCTCTCCTCCAATTCCTTTCTTCTTATGCCCAATCCACACAAGCCTTGCTCATCCCCGGCGGCTGCTTTCTGGTCACCTCCCCCGTCCTCGATTGCCTTCCGGCGCTCAAGCTCCTTGTCACTACTAGCGCCGGTGTTGACCACCTCGACTTGCCGGAATTACGTCGCCGTCAGATCGCTATTGCCTACGTTCCGGACTTGTACTCTGAAGATGTCGCTGATCTTGCGGTTGGGTTGTTGATTGATGTTCTCATGAAGGTTTCGGCGAGAGATCGGTGCTTTAGGCTACGGCTTCCGCCTACTACCAACCCGGAATTTCCGCCTCTCGGATTAAAGTTAAATGGGAAAAGAATTGGGATAGTTGGATTGGGGAAAATAGGGTCTAAAGTTGCCAAAAGGCTGGAGGGATTTGAGTGCAAAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACTACTCCAATGTACATGAACTTGCAAGCAACTGTGATGGCCTCGTACTTTGTTGTGGGTTAACGAAAGAAACCCAACATATGATCAATAGGGAGGTGATGGCTGCATTAGGAAAAGATGGAGTGATAATCAACGTTGGTAGAGGGGCGATAATCGACGAGAAGGCTATGGTTGAATGTTTGATCAAGGGAGAGATTGGGGGAGTTGGGTTAGATGTGTTTGAGAATGAACCTGAAATTCCTGAAGAGCTCTTTAATTTCGATAATGTTGTATTATCACCACATGTTGCTGTTATGACACATGAAACTTTGCAGGGATTGTCTAGGTTGGTGGTGGACAACTTGGAGGCATTGTTCTCAAACAAGCCTTTGGTGTCTCCATTTCCTAATTAG

Coding sequence (CDS)

ATGGGAGAAGAAACTGAAGGCGATAGCAATAGCAACGACCTTCATCAAGTTTTGGTTTTAGGTTCTCCGTGGGTATTACCCGCTCTCCAATCTCAATTCTCAAACCGATTCCATTTCCTCCTACCATCTCTTTCCGATCTCCCTCTCCTCCAATTCCTTTCTTCTTATGCCCAATCCACACAAGCCTTGCTCATCCCCGGCGGCTGCTTTCTGGTCACCTCCCCCGTCCTCGATTGCCTTCCGGCGCTCAAGCTCCTTGTCACTACTAGCGCCGGTGTTGACCACCTCGACTTGCCGGAATTACGTCGCCGTCAGATCGCTATTGCCTACGTTCCGGACTTGTACTCTGAAGATGTCGCTGATCTTGCGGTTGGGTTGTTGATTGATGTTCTCATGAAGGTTTCGGCGAGAGATCGGTGCTTTAGGCTACGGCTTCCGCCTACTACCAACCCGGAATTTCCGCCTCTCGGATTAAAGTTAAATGGGAAAAGAATTGGGATAGTTGGATTGGGGAAAATAGGGTCTAAAGTTGCCAAAAGGCTGGAGGGATTTGAGTGCAAAATCTCATATAACTCAAGGACCAAAAAGCCATTAGTTCCATACTCATACTACTCCAATGTACATGAACTTGCAAGCAACTGTGATGGCCTCGTACTTTGTTGTGGGTTAACGAAAGAAACCCAACATATGATCAATAGGGAGGTGATGGCTGCATTAGGAAAAGATGGAGTGATAATCAACGTTGGTAGAGGGGCGATAATCGACGAGAAGGCTATGGTTGAATGTTTGATCAAGGGAGAGATTGGGGGAGTTGGGTTAGATGTGTTTGAGAATGAACCTGAAATTCCTGAAGAGCTCTTTAATTTCGATAATGTTGTATTATCACCACATGTTGCTGTTATGACACATGAAACTTTGCAGGGATTGTCTAGGTTGGTGGTGGACAACTTGGAGGCATTGTTCTCAAACAAGCCTTTGGTGTCTCCATTTCCTAATTAG
Homology
[Close]
BLAST of CSPI03G09240 vs. Swiss-Prot
Match: HPR3_ARATH (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana GN=HPR3 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.6e-79
Identity = 164/314 (52.23%), Postives = 209/314 (66.56%), Query Frame = 1 

Query: 17  VLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLL-QFLSSYAQSTQALLIPGGCFLVTSP 76
           V++L  P  L  +    +  F  L+   S    L  F   +A S +A +I G    VT  
Sbjct: 9   VVLLHRPPSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVISGR-LPVTDE 68

Query: 77  VLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLIDVLMKVS 136
           +L  LP+L++LV TS G+DH+DL   +RR I I    + +S+DVAD AVGLLI VL ++ 
Sbjct: 69  LLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRRIP 128

Query: 137 ARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKISYNSRTK 196
           A DR  R         +F  LG K++GKR+GIVGLG IGS VAKRLE F C ISYNSR++
Sbjct: 129 AADRYVRSGNWAKFG-DFQ-LGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQ 188

Query: 197 KPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVGRGAIID 256
           K   PY YYS++  LA N D LVLCC LT ET H++NREVM  LGKDGV+INVGRG +ID
Sbjct: 189 KQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLID 248

Query: 257 EKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGLSRLVVD 316
           EK MV+CL+ G IGG GLDVFENEP +P+ELF  DNVVLSPH AV T  +L  ++++ + 
Sbjct: 249 EKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALA 308

Query: 317 NLEALFSNKPLVSP 330
           NL+A FSN+PL+SP
Sbjct: 309 NLKAFFSNRPLLSP 319

[Close]
BLAST of CSPI03G09240 vs. Swiss-Prot
Match: HPPR_PLESU (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides GN=HPPR PE=1 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 2.6e-63
Identity = 131/278 (47.12%), Postives = 187/278 (67.27%), Query Frame = 1 

Query: 52  FLSSYAQSTQALLIPGGCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYV 111
           FL+  A+S +A++         + ++D LP L+++ + S G+D +DL +   + + +   
Sbjct: 38  FLALQAESIRAVVGNSNAG-ADAELIDALPKLEIVSSFSVGLDKVDLIKCEEKGVRVTNT 97

Query: 112 PDLYSEDVADLAVGLLIDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLG 171
           PD+ ++DVADLA+GL++ VL ++   D+   +R       +F  L  K +GKR+GI+GLG
Sbjct: 98  PDVLTDDVADLAIGLILAVLRRICECDKY--VRRGAWKFGDFK-LTTKFSGKRVGIIGLG 157

Query: 172 KIGSKVAKRLEGFECKISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMI 231
           +IG  VA+R E F+C ISY SR+KKP   Y+YY +V ELASN D LV+ C LT ET H+I
Sbjct: 158 RIGLAVAERAEAFDCPISYFSRSKKPNTNYTYYGSVVELASNSDILVVACPLTPETTHII 217

Query: 232 NREVMAALGKDGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDN 291
           NREV+ ALG  GV+IN+GRG  +DE  +V  L++G +GG GLDVFE EPE+PE+LF  +N
Sbjct: 218 NREVIDALGPKGVLINIGRGPHVDEPELVSALVEGRLGGAGLDVFEREPEVPEKLFGLEN 277

Query: 292 VVLSPHVAVMTHETLQGLSRLVVDNLEALFSNKPLVSP 330
           VVL PHV   T ET + ++ LVV NLEA FS KPL++P
Sbjct: 278 VVLLPHVGSGTVETRKVMADLVVGNLEAHFSGKPLLTP 311

[Close]
BLAST of CSPI03G09240 vs. Swiss-Prot
Match: HPR2_ARATH (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana GN=HPR2 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 7.9e-60
Identity = 119/250 (47.60%), Postives = 169/250 (67.60%), Query Frame = 1 

Query: 80  LPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLIDVLMKVSARDR 139
           LP L+++ + S G+D +DL + + + I +   PD+ +EDVADLA+GL++ +L ++   DR
Sbjct: 65  LPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDR 124

Query: 140 CFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKISYNSRTKKPLV 199
              +R       EF  L  K +GK +GI+GLG+IG+ +AKR E F C I+Y SRT KP V
Sbjct: 125 Y--VRSGKWKQGEFQ-LTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDV 184

Query: 200 PYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVGRGAIIDEKAM 259
            Y YY  V +LA N D LV+ C LT++T+H+++R+VM ALG  GV+IN+GRG  +DE+ +
Sbjct: 185 AYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQEL 244

Query: 260 VECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGLSRLVVDNLEA 319
           ++ L +G +GG  LDVFE EP +PEELF  +NVVL PHV   T ET   ++ LVV NLEA
Sbjct: 245 IKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEA 304

Query: 320 LFSNKPLVSP 330
            FS K L++P
Sbjct: 305 HFSGKSLLTP 311

[Close]
BLAST of CSPI03G09240 vs. Swiss-Prot
Match: 2KGR_GLUOX (2-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) GN=GOX0417 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-44
Identity = 103/305 (33.77%), Postives = 166/305 (54.43%), Query Frame = 1 

Query: 25  VLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQSTQALLIPGGCFLVTSPVLDCLPALK 84
           ++P ++ +    F     +L     L+ L + A + + +   GG   V S ++D LP L+
Sbjct: 13  LVPVMKERLEKSF-----TLHPYTSLENLKNIAPAIRGITTGGGSG-VPSEIMDALPNLE 72

Query: 85  LLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLIDVLMKVSARDRCFRLR 144
           ++     G D ++L E RRR I +A   +  ++DVAD+AV L++ V+  +   D   R  
Sbjct: 73  VISVNGVGTDRINLDEARRRNIGVAITQNTLTDDVADMAVALMMAVMRSIVTNDAFVRAG 132

Query: 145 LPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKISYNSRTKKPLVPYSYY 204
             P+      PLG  L  K++GI G G IG  +AKR+  F  +++Y +   +P     + 
Sbjct: 133 KWPSATA---PLGRSLTRKKVGIAGFGHIGQAIAKRVSAFGMEVAYFNSHARPESTCHFE 192

Query: 205 SNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVGRGAIIDEKAMVECLI 264
            ++  LA+ CD L+L       + +MI+R+ + ALGKDG ++N+ RG ++DE A++  L 
Sbjct: 193 PDLKALATWCDVLILAVSGGPRSANMIDRDTLDALGKDGFLVNIARGTVVDEAALLSALQ 252

Query: 265 KGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGLSRLVVDNLEALFSNK 324
           +  I G GLDVF+NEP I     +  N VL  H A  T ET   ++ LVVDNL A F++K
Sbjct: 253 EKRIAGAGLDVFQNEPNINPAFLSLPNTVLQAHQASATVETRTTMANLVVDNLIAYFTDK 308

Query: 325 PLVSP 330
            L++P
Sbjct: 313 TLLTP 308

[Close]
BLAST of CSPI03G09240 vs. Swiss-Prot
Match: GYAR_THEON (Glyoxylate reductase OS=Thermococcus onnurineus (strain NA1) GN=gyaR PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.2e-41
Identity = 95/254 (37.40%), Postives = 143/254 (56.30%), Query Frame = 1 

Query: 72  VTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLIDVL 131
           + + V D  P LK++   + G D++D+ E  +  + I   PD+ +   AD+A  LL+   
Sbjct: 57  IDAEVFDAAPRLKIVANYAVGYDNIDIEEATKMGVYITNTPDVLTNATADMAWVLLLATA 116

Query: 132 MKVSARDRCFRLRLPPTTNPEFPPL---GLKLNGKRIGIVGLGKIGSKVAKRLEGFECKI 191
            ++   D+  R          + PL   G  + G+ IGIVG G+IG  +A+R +GF  +I
Sbjct: 117 RRLIEADKFVRSGEWKKRGVAWHPLMFLGYDVYGRTIGIVGFGRIGQAIARRAKGFGMRI 176

Query: 192 SYNSRTKKPLVPYSY---YSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVI 251
            YNSRT+KP V       +  + EL    D +VL   LTKET HMIN E +  +    ++
Sbjct: 177 LYNSRTRKPEVEKELGAEFMPLDELLKESDFVVLVVPLTKETYHMINEERLKLMKPTAIL 236

Query: 252 INVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHET 311
           +N+ RG ++D +A+V+ L +G I G GLDVFE EP   EELF+ DNVVL+PH+   T+  
Sbjct: 237 VNIARGKVVDTEALVKALREGWIAGAGLDVFEEEPYYHEELFSLDNVVLAPHIGSATYGA 296

Query: 312 LQGLSRLVVDNLEA 320
            +G++ LV  NL A
Sbjct: 297 REGMAELVARNLIA 310

[Close]
BLAST of CSPI03G09240 vs. TrEMBL
Match: A0A0A0L908_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124900 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 3.0e-122
Identity = 226/323 (69.97%), Postives = 267/323 (82.66%), Query Frame = 1 

Query: 8   DSNSNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQSTQALLIPG 67
           +  S +L +VLVLG PWV   L+SQF N+FH+L P LS LPL QFL+SYAQSTQALLIP 
Sbjct: 4   EGESKELPEVLVLGPPWVFSTLESQFPNKFHYLKPWLSQLPLHQFLTSYAQSTQALLIPV 63

Query: 68  GCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLL 127
              L  SP+LDCLP+LKL+VT SAGV+HL+  ELR R IA+AY  +++SEDVAD+AVGLL
Sbjct: 64  SPPL-NSPILDCLPSLKLVVTVSAGVEHLNFAELRGRGIAVAYAGNVFSEDVADMAVGLL 123

Query: 128 IDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECK 187
           IDVL KVSA DR  + RL P   P+FP L  KL+GK+IGIVGLGKIGS+VAKRLEGF C+
Sbjct: 124 IDVLRKVSAGDRFVKQRLQPI-KPDFP-LRSKLSGKQIGIVGLGKIGSEVAKRLEGFGCR 183

Query: 188 ISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIIN 247
           ISYNSRTKKPLV YSYYSNVHELA+NC+ L++CCGLT+ET HMINREVM  LGKDGVIIN
Sbjct: 184 ISYNSRTKKPLVSYSYYSNVHELATNCEVLIICCGLTEETHHMINREVMLELGKDGVIIN 243

Query: 248 VGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQ 307
           +GRGA+IDEK M+ CLI+GEIGG GLDVFE EPEIP++LF  DNVVLSPH AV THE+  
Sbjct: 244 IGRGAVIDEKEMIRCLIEGEIGGAGLDVFEFEPEIPKQLFTLDNVVLSPHTAVTTHESFV 303

Query: 308 GLSRLVVDNLEALFSNKPLVSPF 331
           G+++L V+NLEA FSNKPL+SP+
Sbjct: 304 GIAKLAVENLEAFFSNKPLLSPY 323

[Close]
BLAST of CSPI03G09240 vs. TrEMBL
Match: A0A0A0L4C2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124920 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.3e-117
Identity = 227/321 (70.72%), Postives = 264/321 (82.24%), Query Frame = 1 

Query: 11  SNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQSTQALLI-PGGC 70
           S++LHQVLVL  P V  +L+SQF NRF FL P  S+LPLLQFL S AQS +A L+ PG  
Sbjct: 7   SDELHQVLVLSPPSVFTSLESQFQNRFQFLKPWDSNLPLLQFLISNAQSVRACLVTPGDG 66

Query: 71  FLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLID 130
             V+S +LDCLP+LK +VT SAGVDHL++ ELRRR +AIAY  +L+S+DVAD+AVGLLID
Sbjct: 67  LAVSSAILDCLPSLKFVVTASAGVDHLNVAELRRRGVAIAYAGNLFSQDVADMAVGLLID 126

Query: 131 VLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKIS 190
           VL  VSA DR  R  L   T  +F  LGLKL GKRIGIVGLGKIGS+VAKRLEGF C+IS
Sbjct: 127 VLRNVSAGDRFVRQGLW-ATQMDFS-LGLKLTGKRIGIVGLGKIGSEVAKRLEGFGCRIS 186

Query: 191 YNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVG 250
           YNSRTKKPLVPYS+YSNVHELA+NCD L++C  LT+ET+H+INREVM ALGKDGVIINVG
Sbjct: 187 YNSRTKKPLVPYSHYSNVHELATNCDVLIICSSLTEETRHLINREVMVALGKDGVIINVG 246

Query: 251 RGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGL 310
           RGAIIDEKAM+E LI+GEI G GLDVFE+EPEIP++LFN DNVVLSPHVAV T E++ GL
Sbjct: 247 RGAIIDEKAMIEYLIQGEIKGAGLDVFEDEPEIPKQLFNLDNVVLSPHVAVTTTESIAGL 306

Query: 311 SRLVVDNLEALFSNKPLVSPF 331
             L ++NLEA FSNKPLVSPF
Sbjct: 307 IELALENLEAFFSNKPLVSPF 325

[Close]
BLAST of CSPI03G09240 vs. TrEMBL
Match: A0A0A0L7K6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124910 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.9e-117
Identity = 223/328 (67.99%), Postives = 264/328 (80.49%), Query Frame = 1 

Query: 8   DSNSNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQSTQALLIPG 67
           +  + +L Q+L+LG P + P L+SQFSNRF FL P L +LPL QFL+SYAQSTQALLI G
Sbjct: 4   EEQAKELPQILILGPPSIFPYLESQFSNRFLFLKPWLYNLPLTQFLTSYAQSTQALLIRG 63

Query: 68  GCFL-VTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLA--- 127
           G    +TS ++DCLP+LKL+VT+S GVDHLD PELRRR +AIA   +L+SED AD+A   
Sbjct: 64  GGNTQLTSTIIDCLPSLKLVVTSSVGVDHLDFPELRRRGVAIANAGNLFSEDTADMAGGG 123

Query: 128 --VGLLIDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRL 187
             VGLLIDVL K+SA DR  R  L  +   +FPP GLKL+GKRIGIVGLGKIGS+VAKRL
Sbjct: 124 VAVGLLIDVLRKISAGDRFVRQGLW-SKKEDFPP-GLKLSGKRIGIVGLGKIGSEVAKRL 183

Query: 188 EGFECKISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGK 247
           EGF CKISYNSRTKK + PYSYY NV+ELA+N + L++CC LTKET H+IN+EVM ALGK
Sbjct: 184 EGFGCKISYNSRTKKSMAPYSYYPNVYELAANTEALIICCALTKETYHLINKEVMLALGK 243

Query: 248 DGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVM 307
           DGVI+N+GRG IIDEK M+ CL +GEIGG GLDVFENEP +PEELFN DNVVLSPH AVM
Sbjct: 244 DGVIVNIGRGLIIDEKEMIRCLTQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHAAVM 303

Query: 308 THETLQGLSRLVVDNLEALFSNKPLVSP 330
           T+E+   LS+LVV+NLEA FSNKPLVSP
Sbjct: 304 TYESKVELSKLVVNNLEAFFSNKPLVSP 329

[Close]
BLAST of CSPI03G09240 vs. TrEMBL
Match: E6NU31_JATCU (JHL05D22.12 protein OS=Jatropha curcas GN=JHL05D22.12 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.9e-98
Identity = 196/331 (59.21%), Postives = 242/331 (73.11%), Query Frame = 1 

Query: 5   TEGDSNSNDLHQVLVLGSPWVLPALQ-----SQFSNRFHFLLPSLSDLPLLQFLSSYAQS 64
           T+   +  DL +VL+L  P   P+ Q     S  S +F +L    S LPL QFL+++AQS
Sbjct: 4   TDPQESPEDLPKVLLLKKP---PSFQFFEGESFTSTKFQYLKAYESPLPLHQFLAAHAQS 63

Query: 65  TQALLIPGGCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDV 124
            QA+L  GG   + + +L  LPA++++VTTSAG++ +D+PE RRR IAIA   D+YS DV
Sbjct: 64  VQAVLASGGAS-INADILQLLPAVRVVVTTSAGLNQIDIPECRRRGIAIANAGDVYSADV 123

Query: 125 ADLAVGLLIDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAK 184
           ADLAVGLLIDVL K+SA DR  R  L  T      PLG KL+GKR GIVGLG+IG +VAK
Sbjct: 124 ADLAVGLLIDVLRKISASDRYVRQGLWATKGDY--PLGAKLSGKRAGIVGLGRIGYEVAK 183

Query: 185 RLEGFECKISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAAL 244
           RLE F C ISYNSR KKP V Y +Y NV ELA+NCD L++CCGLTK+T HMIN+EV++AL
Sbjct: 184 RLEAFGCYISYNSRKKKPNVSYPFYQNVSELAANCDALIICCGLTKQTFHMINKEVLSAL 243

Query: 245 GKDGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVA 304
           GK GVI+N+GRGAIIDEK MV CL+ GEI G GLDVFENEP++P+E F  +NVVLSPH A
Sbjct: 244 GKKGVIVNIGRGAIIDEKEMVRCLVAGEIAGAGLDVFENEPDVPKEFFTMENVVLSPHTA 303

Query: 305 VMTHETLQGLSRLVVDNLEALFSNKPLVSPF 331
           V T E+L+ LS LVV NLEA FSNKPL+S +
Sbjct: 304 VFTPESLKDLSELVVGNLEAFFSNKPLLSEY 328

[Close]
BLAST of CSPI03G09240 vs. TrEMBL
Match: M5XK89_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025117mg PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 9.0e-95
Identity = 185/322 (57.45%), Postives = 230/322 (71.43%), Query Frame = 1 

Query: 8   DSNSNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQSTQALLIPG 67
           + N ++L QVLVL  P     L+S +S++F FL    S LP  QFL+++A S QALL   
Sbjct: 4   NGNHSELPQVLVLLPPGCFTLLESNYSHKFKFLKAWDSPLPQDQFLATHAGSVQALLSSA 63

Query: 68  GCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLL 127
               +T+ +L  LP+LK++VTTS G+DHLDL E R R +AIA  P +++EDVAD+AVGLL
Sbjct: 64  NGPTITANILQMLPSLKVIVTTSVGIDHLDLAECRSRGVAIASTPKIFTEDVADMAVGLL 123

Query: 128 IDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECK 187
           +DV+ K+SA DR  R  L  T       LG K+ GKR+GIVGLG IG +VAKRLE F C 
Sbjct: 124 LDVMRKISAGDRYVRDGLWATRGDY--ALGSKIGGKRVGIVGLGNIGLEVAKRLEAFGCN 183

Query: 188 ISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIIN 247
           I YNSRT+KP V Y +YS++ ELA+N D LV+CC LT ET H+IN++V+ ALG+DGVI+N
Sbjct: 184 ILYNSRTEKPFVSYPFYSDICELAANSDALVICCALTAETHHLINKKVLLALGRDGVIVN 243

Query: 248 VGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQ 307
           +GRGAIIDEK MV CL+ GEIGG GLDVFE+EPE+PEELF  DNVVLSPH A  T E   
Sbjct: 244 IGRGAIIDEKEMVRCLVIGEIGGAGLDVFEDEPEVPEELFALDNVVLSPHYATYTPECFM 303

Query: 308 GLSRLVVDNLEALFSNKPLVSP 330
            L  LV  N EA FSNKPL+SP
Sbjct: 304 ALCELVAGNFEAFFSNKPLLSP 323

[Close]
BLAST of CSPI03G09240 vs. TAIR10
Match: AT2G45630.2 (AT2G45630.2 D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 316.6 bits (810), Expect = 1.8e-86
Identity = 171/324 (52.78%), Postives = 226/324 (69.75%), Query Frame = 1 

Query: 9   SNSNDLHQVLVLGSPWVLPALQSQF--SNRFHFLLPSLSDLPLLQFLSSYAQSTQALLIP 68
           S++  L +VL++  P  +  L   F  S +F  L    S LPL +FL+ ++ S  A++ P
Sbjct: 16  SSTEKLPRVLIVKRPDAMAVLGDGFVASTKFEILKAFESPLPLPEFLAYHSDSISAIIAP 75

Query: 69  GGCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGL 128
                VT+ ++  LP L+L+VTTSAGVDH+DL E RRR I++A     +SEDVAD AVGL
Sbjct: 76  VAA-PVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVADTAVGL 135

Query: 129 LIDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFEC 188
           LIDV  ++SA +R  + R  P       PLG KL  KRIGIVGLG IGSKVA RL+ F C
Sbjct: 136 LIDVFRRISAANRFVKQRFWPLKGDY--PLGSKLGRKRIGIVGLGSIGSKVATRLDAFGC 195

Query: 189 KISYNSRTKKPL-VPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVI 248
           +ISY+SR +KP  VPY YY ++ E+A+N D L++CC L ++T  +IN++V++ALGK GVI
Sbjct: 196 QISYSSRNRKPYDVPYHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKRGVI 255

Query: 249 INVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHET 308
           +NV RGAIIDE+ MV CL +GEIGG GLDVFE+EP +P+ELF  DNVV SPH A MT E 
Sbjct: 256 VNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKELFELDNVVFSPHSAFMTLEG 315

Query: 309 LQGLSRLVVDNLEALFSNKPLVSP 330
           L+ L ++VV N+EA FSNKPL++P
Sbjct: 316 LEELGKVVVGNIEAFFSNKPLLTP 336

[Close]
BLAST of CSPI03G09240 vs. TAIR10
Match: AT1G12550.1 (AT1G12550.1 D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 297.0 bits (759), Expect = 1.5e-80
Identity = 164/314 (52.23%), Postives = 209/314 (66.56%), Query Frame = 1 

Query: 17  VLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLL-QFLSSYAQSTQALLIPGGCFLVTSP 76
           V++L  P  L  +    +  F  L+   S    L  F   +A S +A +I G    VT  
Sbjct: 9   VVLLHRPPSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVISGR-LPVTDE 68

Query: 77  VLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLIDVLMKVS 136
           +L  LP+L++LV TS G+DH+DL   +RR I I    + +S+DVAD AVGLLI VL ++ 
Sbjct: 69  LLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRRIP 128

Query: 137 ARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKISYNSRTK 196
           A DR  R         +F  LG K++GKR+GIVGLG IGS VAKRLE F C ISYNSR++
Sbjct: 129 AADRYVRSGNWAKFG-DFQ-LGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQ 188

Query: 197 KPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVGRGAIID 256
           K   PY YYS++  LA N D LVLCC LT ET H++NREVM  LGKDGV+INVGRG +ID
Sbjct: 189 KQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLID 248

Query: 257 EKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGLSRLVVD 316
           EK MV+CL+ G IGG GLDVFENEP +P+ELF  DNVVLSPH AV T  +L  ++++ + 
Sbjct: 249 EKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALA 308

Query: 317 NLEALFSNKPLVSP 330
           NL+A FSN+PL+SP
Sbjct: 309 NLKAFFSNRPLLSP 319

[Close]
BLAST of CSPI03G09240 vs. TAIR10
Match: AT1G79870.1 (AT1G79870.1 D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 232.3 bits (591), Expect = 4.5e-61
Identity = 119/250 (47.60%), Postives = 169/250 (67.60%), Query Frame = 1 

Query: 80  LPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLIDVLMKVSARDR 139
           LP L+++ + S G+D +DL + + + I +   PD+ +EDVADLA+GL++ +L ++   DR
Sbjct: 65  LPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDR 124

Query: 140 CFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKISYNSRTKKPLV 199
              +R       EF  L  K +GK +GI+GLG+IG+ +AKR E F C I+Y SRT KP V
Sbjct: 125 Y--VRSGKWKQGEFQ-LTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDV 184

Query: 200 PYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVGRGAIIDEKAM 259
            Y YY  V +LA N D LV+ C LT++T+H+++R+VM ALG  GV+IN+GRG  +DE+ +
Sbjct: 185 AYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQEL 244

Query: 260 VECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGLSRLVVDNLEA 319
           ++ L +G +GG  LDVFE EP +PEELF  +NVVL PHV   T ET   ++ LVV NLEA
Sbjct: 245 IKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLEA 304

Query: 320 LFSNKPLVSP 330
            FS K L++P
Sbjct: 305 HFSGKSLLTP 311

[Close]
BLAST of CSPI03G09240 vs. TAIR10
Match: AT5G28310.1 (AT5G28310.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 122.9 bits (307), Expect = 3.8e-28
Identity = 76/183 (41.53%), Postives = 96/183 (52.46%), Query Frame = 1 

Query: 148 TTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKISYNSRTKKPL-VPYSYYSN 207
           T+  + P L    + KRIGIVGLG IGSKVA RL+ F C+ISY+SR +KP  VPY YY +
Sbjct: 100 TSGYDDPDLNQYQSKKRIGIVGLGSIGSKVATRLKAFGCQISYSSRNRKPYAVPYHYYMD 159

Query: 208 VHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVGRGAIIDEKAMVECLIKG 267
           + E+                                GVI+NV  GAIIDE+ M       
Sbjct: 160 IEEM-------------------------------HGVIVNVALGAIIDEEEM------- 219

Query: 268 EIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGLSRLVVDNLEALFSNKPL 327
                          +P+ELF  DNVV SPH A MT E L+ L ++VV N+EA FSNKPL
Sbjct: 220 -------------SNVPKELFELDNVVFSPHCAFMTLEGLEELGKVVVGNIEAFFSNKPL 231

Query: 328 VSP 330
           ++P
Sbjct: 280 LTP 231

[Close]
BLAST of CSPI03G09240 vs. TAIR10
Match: AT1G72190.1 (AT1G72190.1 D-isomer specific 2-hydroxyacid dehydrogenase family protein)

HSP 1 Score: 112.1 bits (279), Expect = 6.7e-25
Identity = 73/266 (27.44%), Postives = 137/266 (51.50%), Query Frame = 1 

Query: 74  SPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVA---DLAVGLLIDV 133
           S V+     +KL++    G+D +D+    +  I +A +P   + + A   ++A+ L++ +
Sbjct: 107 SNVISRASNIKLIMQYGVGLDGVDIDAATKHGIKVARIPSEGTGNAASCSEMAIYLMLGL 166

Query: 134 LMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKISY 193
           L K +      R RL         P G  L GK + I+G G IG ++AKRL+ F  ++  
Sbjct: 167 LKKQNEMQISLRNRLLGE------PTGDTLLGKTVFILGYGNIGIELAKRLKPFGSRVIA 226

Query: 194 NSRTKKPLVPYS---------YYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGK 253
             R     +  S          + +++  A   D +V+C  L KET  ++N+E + ++ K
Sbjct: 227 TKRFWPASIVDSDSRLVDEKGSHEDIYTFAGKADIVVVCLRLNKETAEIVNKEFICSMKK 286

Query: 254 DGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEE-LFNFDNVVLSPHVAV 313
             +++N+ RG +I+ ++  + L  G +GG+G+DV  +EP  P + +  F NV+++PHVA 
Sbjct: 287 GALLVNIARGGLINYESAFQNLESGHLGGLGIDVAWSEPFDPNDPILKFKNVIITPHVAG 346

Query: 314 MTHETLQGLSRLVVDNLEALFSNKPL 327
           +T  + + ++++V D    L    PL
Sbjct: 347 VTEYSYRSMAKIVGDLALQLHEGLPL 366

[Close]
BLAST of CSPI03G09240 vs. NCBI nr
Match: gi|778677348|ref|XP_004133892.2| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis sativus])

HSP 1 Score: 663.3 bits (1710), Expect = 2.2e-187
Identity = 331/332 (99.70%), Postives = 332/332 (100.00%), Query Frame = 1 

Query: 1   MGEETEGDSNSNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQST 60
           MGEETEGDSNSNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQST
Sbjct: 1   MGEETEGDSNSNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQST 60

Query: 61  QALLIPGGCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVA 120
           QALLIPGGCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVA
Sbjct: 61  QALLIPGGCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVA 120

Query: 121 DLAVGLLIDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKR 180
           DLAVGLLIDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKR
Sbjct: 121 DLAVGLLIDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKR 180

Query: 181 LEGFECKISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALG 240
           LEGFECKISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALG
Sbjct: 181 LEGFECKISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALG 240

Query: 241 KDGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAV 300
           KDGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAV
Sbjct: 241 KDGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAV 300

Query: 301 MTHETLQGLSRLVVDNLEALFSNKPLVSPFPN 333
           MTHETL+GLSRLVVDNLEALFSNKPLVSPFPN
Sbjct: 301 MTHETLEGLSRLVVDNLEALFSNKPLVSPFPN 332

[Close]
BLAST of CSPI03G09240 vs. NCBI nr
Match: gi|659075382|ref|XP_008438113.1| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo])

HSP 1 Score: 607.4 bits (1565), Expect = 1.4e-170
Identity = 306/332 (92.17%), Postives = 318/332 (95.78%), Query Frame = 1 

Query: 1   MGEETEGDSNSNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQST 60
           M EE+EG  +  +LHQVLVLGSPWVLPAL+SQFSNRFHFL+PSLSDLPLLQFLSSYAQST
Sbjct: 1   MEEESEG--HGKNLHQVLVLGSPWVLPALRSQFSNRFHFLIPSLSDLPLLQFLSSYAQST 60

Query: 61  QALLIPGGCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVA 120
           QALLIPGGCFLVTS VLDCLPALKLLVTTSAGVDHLDLPELRRR+I IAYVPDLYSEDVA
Sbjct: 61  QALLIPGGCFLVTSAVLDCLPALKLLVTTSAGVDHLDLPELRRRRIGIAYVPDLYSEDVA 120

Query: 121 DLAVGLLIDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKR 180
           DLAVGLLIDVLMKVSA DR FRLRLPPTTN +FPP  LKLNGKRIGIVGLG+IGSKVAKR
Sbjct: 121 DLAVGLLIDVLMKVSAGDRFFRLRLPPTTNGDFPPFRLKLNGKRIGIVGLGQIGSKVAKR 180

Query: 181 LEGFECKISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALG 240
           LEGF+CKISYNSRTKKPLVPYS+YSNVHELASNCDGLV+CCGLTKETQHMI+REVM ALG
Sbjct: 181 LEGFDCKISYNSRTKKPLVPYSFYSNVHELASNCDGLVICCGLTKETQHMIDREVMVALG 240

Query: 241 KDGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAV 300
           KDGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAV
Sbjct: 241 KDGVIINVGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAV 300

Query: 301 MTHETLQGLSRLVVDNLEALFSNKPLVSPFPN 333
           MTHETL GLSRLVVDNLEALFSNKPLVSPFPN
Sbjct: 301 MTHETLVGLSRLVVDNLEALFSNKPLVSPFPN 330

[Close]
BLAST of CSPI03G09240 vs. NCBI nr
Match: gi|449433109|ref|XP_004134340.1| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus])

HSP 1 Score: 446.4 bits (1147), Expect = 4.3e-122
Identity = 226/323 (69.97%), Postives = 267/323 (82.66%), Query Frame = 1 

Query: 8   DSNSNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQSTQALLIPG 67
           +  S +L +VLVLG PWV   L+SQF N+FH+L P LS LPL QFL+SYAQSTQALLIP 
Sbjct: 4   EGESKELPEVLVLGPPWVFSTLESQFPNKFHYLKPWLSQLPLHQFLTSYAQSTQALLIPV 63

Query: 68  GCFLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLL 127
              L  SP+LDCLP+LKL+VT SAGV+HL+  ELR R IA+AY  +++SEDVAD+AVGLL
Sbjct: 64  SPPL-NSPILDCLPSLKLVVTVSAGVEHLNFAELRGRGIAVAYAGNVFSEDVADMAVGLL 123

Query: 128 IDVLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECK 187
           IDVL KVSA DR  + RL P   P+FP L  KL+GK+IGIVGLGKIGS+VAKRLEGF C+
Sbjct: 124 IDVLRKVSAGDRFVKQRLQPI-KPDFP-LRSKLSGKQIGIVGLGKIGSEVAKRLEGFGCR 183

Query: 188 ISYNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIIN 247
           ISYNSRTKKPLV YSYYSNVHELA+NC+ L++CCGLT+ET HMINREVM  LGKDGVIIN
Sbjct: 184 ISYNSRTKKPLVSYSYYSNVHELATNCEVLIICCGLTEETHHMINREVMLELGKDGVIIN 243

Query: 248 VGRGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQ 307
           +GRGA+IDEK M+ CLI+GEIGG GLDVFE EPEIP++LF  DNVVLSPH AV THE+  
Sbjct: 244 IGRGAVIDEKEMIRCLIEGEIGGAGLDVFEFEPEIPKQLFTLDNVVLSPHTAVTTHESFV 303

Query: 308 GLSRLVVDNLEALFSNKPLVSPF 331
           G+++L V+NLEA FSNKPL+SP+
Sbjct: 304 GIAKLAVENLEAFFSNKPLLSPY 323

[Close]
BLAST of CSPI03G09240 vs. NCBI nr
Match: gi|659075398|ref|XP_008438122.1| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo])

HSP 1 Score: 436.4 bits (1121), Expect = 4.4e-119
Identity = 225/321 (70.09%), Postives = 267/321 (83.18%), Query Frame = 1 

Query: 11  SNDLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQSTQALLI-PGGC 70
           S++LHQVLVL  P+   +L+SQF NRF FL P  S+LPLLQFL S AQS +A L+ PGG 
Sbjct: 7   SDELHQVLVLSPPFEFTSLESQFQNRFQFLNPWNSNLPLLQFLISNAQSVRACLVMPGGR 66

Query: 71  FLVTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLID 130
             V+S +LDCLP+L+L+VTTSAGVDHL + E+RRR +AIA+  +L S+DVAD+AVGLLID
Sbjct: 67  LAVSSAILDCLPSLELVVTTSAGVDHLHVAEIRRRGVAIAFAGNLLSQDVADMAVGLLID 126

Query: 131 VLMKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKIS 190
           VL  +SA DR  R  L PT   +F  +GLKL+GKRIGIVGLGKIGS+VAKRLEGF C+IS
Sbjct: 127 VLRNISAGDRFVRQGLWPTQG-DFS-IGLKLSGKRIGIVGLGKIGSEVAKRLEGFGCRIS 186

Query: 191 YNSRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVG 250
           YNSRTKKPLVPYS+YSNVHELA+NCD L++CC LT+ET+H+INREVM ALGKDGVIINVG
Sbjct: 187 YNSRTKKPLVPYSHYSNVHELATNCDALIICCCLTEETRHLINREVMVALGKDGVIINVG 246

Query: 251 RGAIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGL 310
           RGAIIDEKAM+ECLI+GEI G GLDVFE+EPEIP++LFN DNVVLSPH A  T E++ GL
Sbjct: 247 RGAIIDEKAMIECLIQGEIKGAGLDVFEHEPEIPKQLFNLDNVVLSPHAAGTTTESIVGL 306

Query: 311 SRLVVDNLEALFSNKPLVSPF 331
           + L  +NLEA FSNKPLVSPF
Sbjct: 307 TELAFENLEAFFSNKPLVSPF 325

[Close]
BLAST of CSPI03G09240 vs. NCBI nr
Match: gi|659075400|ref|XP_008438123.1| (PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo])

HSP 1 Score: 434.1 bits (1115), Expect = 2.2e-118
Identity = 226/318 (71.07%), Postives = 262/318 (82.39%), Query Frame = 1 

Query: 13  DLHQVLVLGSPWVLPALQSQFSNRFHFLLPSLSDLPLLQFLSSYAQSTQALLIPGG-CFL 72
           +L QVLVLG P + P L+SQF NRF FL P L +LPL QFL+SYAQSTQALLI GG    
Sbjct: 2   ELPQVLVLGPPSIFPYLESQFPNRFFFLKPWLYNLPLTQFLTSYAQSTQALLIRGGGSTQ 61

Query: 73  VTSPVLDCLPALKLLVTTSAGVDHLDLPELRRRQIAIAYVPDLYSEDVADLAVGLLIDVL 132
           +TS ++DCLP+LKL+VT+S GVDHLDLPELRRR +AIA   +L+SED AD+AVGLLIDVL
Sbjct: 62  LTSAIIDCLPSLKLVVTSSVGVDHLDLPELRRRGVAIANAGNLFSEDAADMAVGLLIDVL 121

Query: 133 MKVSARDRCFRLRLPPTTNPEFPPLGLKLNGKRIGIVGLGKIGSKVAKRLEGFECKISYN 192
            KVSA DR  R  L  +   +FPP GLKL+GKRIGIVGLGKIGS+VAKRLEGF CK+SYN
Sbjct: 122 RKVSAGDRFVRQGLW-SKKGDFPP-GLKLSGKRIGIVGLGKIGSEVAKRLEGFGCKVSYN 181

Query: 193 SRTKKPLVPYSYYSNVHELASNCDGLVLCCGLTKETQHMINREVMAALGKDGVIINVGRG 252
           SRTKK + PYSYYSNV+ELA+N + L++CC LTKET  +IN+EVM ALGKDGVI+NVGRG
Sbjct: 182 SRTKKSMAPYSYYSNVYELAANTEALIICCALTKETYRLINKEVMQALGKDGVIVNVGRG 241

Query: 253 AIIDEKAMVECLIKGEIGGVGLDVFENEPEIPEELFNFDNVVLSPHVAVMTHETLQGLSR 312
            IIDEK M+ CLI+GEIGG GLDVFENEP +PEELFN DNVVLSPH AVMT+E+   LS+
Sbjct: 242 LIIDEKEMIRCLIQGEIGGAGLDVFENEPNVPEELFNLDNVVLSPHNAVMTYESKVELSK 301

Query: 313 LVVDNLEALFSNKPLVSP 330
           LVV+NLEA FSN PLVSP
Sbjct: 302 LVVNNLEAFFSNTPLVSP 317

The following BLAST results are available for this feature:
BLAST of CSPI03G09240 vs. Swiss-Prot
Match NameE-valueIdentityDescription
HPR3_ARATH2.6e-7952.23Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana GN=HPR3 PE=2 S... [more]
HPPR_PLESU2.6e-6347.12Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides GN=HPPR PE=1 SV=... [more]
HPR2_ARATH7.9e-6047.60Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana GN=HPR2 PE=1... [more]
2KGR_GLUOX1.2e-4433.772-ketogluconate reductase OS=Gluconobacter oxydans (strain 621H) GN=GOX0417 PE=1... [more]
GYAR_THEON2.2e-4137.40Glyoxylate reductase OS=Thermococcus onnurineus (strain NA1) GN=gyaR PE=3 SV=1[more]
BLAST of CSPI03G09240 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L908_CUCSA3.0e-12269.97Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124900 PE=3 SV=1[more]
A0A0A0L4C2_CUCSA1.3e-11770.72Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124920 PE=3 SV=1[more]
A0A0A0L7K6_CUCSA2.9e-11767.99Uncharacterized protein OS=Cucumis sativus GN=Csa_3G124910 PE=3 SV=1[more]
E6NU31_JATCU3.9e-9859.21JHL05D22.12 protein OS=Jatropha curcas GN=JHL05D22.12 PE=3 SV=1[more]
M5XK89_PRUPE9.0e-9557.45Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025117mg PE=3 SV=1[more]
BLAST of CSPI03G09240 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G45630.21.8e-8652.78 D-isomer specific 2-hydroxyacid dehydrogenase family protein[more]
AT1G12550.11.5e-8052.23 D-isomer specific 2-hydroxyacid dehydrogenase family protein[more]
AT1G79870.14.5e-6147.60 D-isomer specific 2-hydroxyacid dehydrogenase family protein[more]
AT5G28310.13.8e-2841.53 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT1G72190.16.7e-2527.44 D-isomer specific 2-hydroxyacid dehydrogenase family protein[more]
BLAST of CSPI03G09240 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778677348|ref|XP_004133892.2|2.2e-18799.70PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis sativus][more]
gi|659075382|ref|XP_008438113.1|1.4e-17092.17PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo][more]
gi|449433109|ref|XP_004134340.1|4.3e-12269.97PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3 [Cucumis sativus][more]
gi|659075398|ref|XP_008438122.1|4.4e-11970.09PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo][more]
gi|659075400|ref|XP_008438123.1|2.2e-11871.07PREDICTED: glyoxylate/hydroxypyruvate reductase HPR3-like [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006139D-isomer_2_OHA_DH_cat_dom
IPR006140D-isomer_DH_NAD-bd
IPR016040NAD(P)-bd_dom
Vocabulary: Molecular Function
TermDefinition
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0051287NAD binding
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G09240.1CSPI03G09240.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 72..326
score: 2.3
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 154..299
score: 1.2
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 130..302
score: 5.7E-49coord: 17..129
score: 7.3
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 116..299
score: 1.63
NoneNo IPR availablePANTHERPTHR109962-HYDROXYACID DEHYDROGENASE-RELATEDcoord: 4..329
score: 3.5E
NoneNo IPR availablePANTHERPTHR10996:SF155D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE-LIKE PROTEIN-RELATEDcoord: 4..329
score: 3.5E
NoneNo IPR availableunknownSSF52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 25..126
score: 9.99

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI03G09240Cla021276Watermelon (97103) v1cpiwmB231
CSPI03G09240MELO3C006155Melon (DHL92) v3.5.1cpimeB230
CSPI03G09240ClCG05G001950Watermelon (Charleston Gray)cpiwcgB226
CSPI03G09240Lsi05G019950Bottle gourd (USVL1VR-Ls)cpilsiB207
CSPI03G09240MELO3C006155.2Melon (DHL92) v3.6.1cpimedB224
CSPI03G09240CsaV3_3G009360Cucumber (Chinese Long) v3cpicucB144
CSPI03G09240Cla97C05G082390Watermelon (97103) v2cpiwmbB209
CSPI03G09240Bhi01G000222Wax gourdcpiwgoB287
CSPI03G09240Cucsa.257950Cucumber (Gy14) v1cgycpiB383
CSPI03G09240CmaCh06G011140Cucurbita maxima (Rimu)cmacpiB826
CSPI03G09240CmoCh06G011410Cucurbita moschata (Rifu)cmocpiB814
CSPI03G09240CsGy3G009320Cucumber (Gy14) v2cgybcpiB110
CSPI03G09240Carg23886Silver-seed gourdcarcpiB0525
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI03G09240Watermelon (97103) v2cpiwmbB178
CSPI03G09240Watermelon (97103) v2cpiwmbB234
CSPI03G09240Wild cucumber (PI 183967)cpicpiB105
CSPI03G09240Wild cucumber (PI 183967)cpicpiB111
CSPI03G09240Wild cucumber (PI 183967)cpicpiB126
CSPI03G09240Cucumber (Gy14) v1cgycpiB455
CSPI03G09240Cucumber (Gy14) v1cgycpiB537
CSPI03G09240Cucurbita maxima (Rimu)cmacpiB255
CSPI03G09240Cucurbita maxima (Rimu)cmacpiB292
CSPI03G09240Cucurbita maxima (Rimu)cmacpiB906
CSPI03G09240Cucurbita moschata (Rifu)cmocpiB243
CSPI03G09240Cucurbita moschata (Rifu)cmocpiB283
CSPI03G09240Cucurbita moschata (Rifu)cmocpiB886
CSPI03G09240Cucumber (Chinese Long) v2cpicuB120
CSPI03G09240Cucumber (Chinese Long) v2cpicuB150
CSPI03G09240Melon (DHL92) v3.5.1cpimeB167
CSPI03G09240Melon (DHL92) v3.5.1cpimeB177
CSPI03G09240Melon (DHL92) v3.5.1cpimeB249
CSPI03G09240Watermelon (Charleston Gray)cpiwcgB250
CSPI03G09240Watermelon (97103) v1cpiwmB251
CSPI03G09240Watermelon (97103) v1cpiwmB259
CSPI03G09240Watermelon (97103) v1cpiwmB278
CSPI03G09240Cucurbita pepo (Zucchini)cpecpiB315
CSPI03G09240Cucurbita pepo (Zucchini)cpecpiB611
CSPI03G09240Cucurbita pepo (Zucchini)cpecpiB861
CSPI03G09240Bottle gourd (USVL1VR-Ls)cpilsiB153
CSPI03G09240Melon (DHL92) v3.6.1cpimedB158
CSPI03G09240Melon (DHL92) v3.6.1cpimedB168
CSPI03G09240Melon (DHL92) v3.6.1cpimedB246
CSPI03G09240Cucumber (Gy14) v2cgybcpiB209
CSPI03G09240Cucumber (Gy14) v2cgybcpiB264
CSPI03G09240Silver-seed gourdcarcpiB0651
CSPI03G09240Silver-seed gourdcarcpiB0974
CSPI03G09240Silver-seed gourdcarcpiB1116
CSPI03G09240Cucumber (Chinese Long) v3cpicucB164
CSPI03G09240Cucumber (Chinese Long) v3cpicucB178