CSPI03G00510 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G00510
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Aconitate hydratase
Location	Chr3 : 373277 .. 374523 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGGTCCCACAGAGCGGAAGTAATTAATTTGGATACAAAGGACTTTACACACTTTATATGGGTTCCTCATGAGCTAAGTTCAGGTGTGTGATTTGTGAATTTGTAAGGTTGTGATTTACATGTCTATTTGGATTACTTTTTAGACACTTAAAAAGTCATTTCAAACAAAATGGTACAATATAGACAAAAAGGAAAAAGTAAGTCTAAATTTGGATATGTTGCAGAGAGGTTACCATTTTCAATTAGAGTGCTTCTGGAATCGGCAATACACAACTGTGACGAATTTCAGGTGAAGTTTAAGGATGTTGAGAAGATTCTTGATTGGGAAAATACTTGTCCCAGACAGGTTGAAATCCCATTTAAGCCTTGCCAGAGTCCTGCTTCAGGTGTATTAAAAGTGTAGCCAATAGTTTAATTGAGGTTGTAAGTTTGTGAAGTTACGATTAATTTGAATTGATTGATGGTGATCAAGATTTCACTGGACTGCCTGCTGTCATTGATCTTGCTTGCATGAAAGATGCACTCAACAACATTGGTGGCAACTCCAACAAGGTGATTCCATTGGTGGGTGAAAAACTTACCTCCAAACTTCGAAGTCAGAAGTTTGGATCTTCTTCCAAAAACAAACCATCTGTGAATGTATCACATTTATGTCTAAAAAGCAAAAGAAAAATGTGCTATGCGTATTTCAAGTAAGTGTGTAATCAGATAATGAATAAAGGGGTAGAAAGAAAGATTAGTAAAATAAAGTGGGTAGGAAGAAAAATAGTACCACTTAAGTACTTTCTGGTTTTTTTAGAAGATGTGTTTTTAAGTTATAGTTCCAACACAAAGACGAGTAGTAGAATTGTTTTAATCCTTTAAGGGTATGTTGAAACATATCCAAACTAAATTTAAAGGCTGATTTATATATGTAAACTAGTCCTTGGATAATGCAGATTCCTGTAGATCTTGTGATTGACCATTCAGTAGAGGTTGACATGGCAAGATCAGAAAAGGCTGTGGAGGCCAATATGGAGCTTGATTTCCATAGAAATAAAGAAAGATTTGCTTTTCTCAAATGAGGATCCAATGCATTCCACAACATGCTCATAGTTCCAACTCGATCCATCCACAAATACCCATAATGGAGGTTATATGGGCATGAATTTGCATGTTAATCTTGAACATCTTGGGAGAGTTGTGTTCGACAGAGGAGGCATGTTTTACCCACAGTGTTATAGGGATAGATTCACATACCAC

mRNA sequence

ATGAAGAGGTCCCACAGAGCGGAAGTAATTAATTTGGATACAAAGGACTTTACACACTTTATATGGGTTCCTCATGAGCTAAGTTCAGAGAGGTTACCATTTTCAATTAGAGTGCTTCTGGAATCGGCAATACACAACTGTGACGAATTTCAGGTGAAGTTTAAGGATGTTGAGAAGATTCTTGATTGGGAAAATACTTGTCCCAGACAGGTTGAAATCCCATTTAAGCCTTGCCAGAGTCCTGCTTCAGATTTCACTGGACTGCCTGCTGTCATTGATCTTGCTTGCATGAAAGATGCACTCAACAACATTGGTGGCAACTCCAACAAGGTGATTCCATTGGTGGGTGAAAAACTTACCTCCAAACTTCGAAGTCAGAAGTTTGGATCTTCTTCCAAAAACAAACCATCTGTGAATATTCCTGTAGATCTTGTGATTGACCATTCAGTAGAGGTTGACATGGCAAGATCAGAAAAGGCTGTGGAGGCCAATATGGAGCTTGATTTCCATAGAAATAAAGAAAGATTTGCTTTTCTCAAATGA

Coding sequence (CDS)

ATGAAGAGGTCCCACAGAGCGGAAGTAATTAATTTGGATACAAAGGACTTTACACACTTTATATGGGTTCCTCATGAGCTAAGTTCAGAGAGGTTACCATTTTCAATTAGAGTGCTTCTGGAATCGGCAATACACAACTGTGACGAATTTCAGGTGAAGTTTAAGGATGTTGAGAAGATTCTTGATTGGGAAAATACTTGTCCCAGACAGGTTGAAATCCCATTTAAGCCTTGCCAGAGTCCTGCTTCAGATTTCACTGGACTGCCTGCTGTCATTGATCTTGCTTGCATGAAAGATGCACTCAACAACATTGGTGGCAACTCCAACAAGGTGATTCCATTGGTGGGTGAAAAACTTACCTCCAAACTTCGAAGTCAGAAGTTTGGATCTTCTTCCAAAAACAAACCATCTGTGAATATTCCTGTAGATCTTGTGATTGACCATTCAGTAGAGGTTGACATGGCAAGATCAGAAAAGGCTGTGGAGGCCAATATGGAGCTTGATTTCCATAGAAATAAAGAAAGATTTGCTTTTCTCAAATGA

Homology

[Close]

BLAST of CSPI03G00510 vs. Swiss-Prot
Match: ACO1_ARATH (Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 1.5e-49
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCDEFQVK KDVEKILDWENT P+QVEIPFKP +    DFTG+P
Sbjct: 37  DKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 157 VQVDVARSENAVQANMELEFQRNKERFAFLK 162

[Close]

BLAST of CSPI03G00510 vs. Swiss-Prot
Match: ACOC_CUCMA (Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.2e-46
Identity = 93/151 (61.59%), Postives = 112/151 (74.17%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           +RLP+SIR+LLESAI NCD FQVK +DVEKI+DWEN+ P+QVEIPFKP +    DFTG+P
Sbjct: 37  DRLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+N +G +SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNKLGSDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 157 VQVDVARSENAVQANMELEFQRNKERFAFLK 162

[Close]

BLAST of CSPI03G00510 vs. Swiss-Prot
Match: ACO2M_ARATH (Aconitate hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=ACO2 PE=1 SV=2)

HSP 1 Score: 183.3 bits (464), Expect = 2.3e-45
Identity = 91/151 (60.26%), Postives = 110/151 (72.85%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCD FQV  +DVEKI+DWE T P+QVEIPFKP +    DFTG+P
Sbjct: 129 DKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSPKQVEIPFKPARVLLQDFTGVP 188

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+N +G +SNK+ PLV                         PVDLVIDHS
Sbjct: 189 AVVDLACMRDAMNKLGSDSNKINPLV-------------------------PVDLVIDHS 248

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 249 VQVDVARSENAVQANMELEFQRNKERFAFLK 254

[Close]

BLAST of CSPI03G00510 vs. Swiss-Prot
Match: ACOC_ORYSJ (Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os08g0191100 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.9e-40
Identity = 83/151 (54.97%), Postives = 103/151 (68.21%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCD FQV   DVEKI+DWENT P+  EIPFKP +    DFTG+P
Sbjct: 37  DKLPYSIRILLESAIRNCDNFQVNQNDVEKIIDWENTSPKLAEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLA M+DA+  +G ++NK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLAAMRDAMAKLGSDANKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARS  AV++NMEL+F RN ERF FLK
Sbjct: 157 VQVDVARSPNAVQSNMELEFKRNNERFGFLK 162

[Close]

BLAST of CSPI03G00510 vs. Swiss-Prot
Match: ACO3M_ARATH (Aconitate hydratase 3, mitochondrial OS=Arabidopsis thaliana GN=ACO3 PE=2 SV=3)

HSP 1 Score: 160.2 bits (404), Expect = 2.1e-38
Identity = 79/151 (52.32%), Postives = 101/151 (66.89%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LPFS+R+LLESAI NCD +QV   DVEKILDWENT  +QVEI FKP +    DFTG+P
Sbjct: 134 DKLPFSVRILLESAIRNCDNYQVTKDDVEKILDWENTSTKQVEIAFKPARVILQDFTGVP 193

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
            ++DLA M+DA+ N+G + +K+ PLV                         PVDLV+DHS
Sbjct: 194 VLVDLASMRDAVKNLGSDPSKINPLV-------------------------PVDLVVDHS 253

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           ++VD ARSE A + N+EL+F RNKERF FLK
Sbjct: 254 IQVDFARSEDAAQKNLELEFKRNKERFTFLK 259

[Close]

BLAST of CSPI03G00510 vs. TrEMBL
Match: A0A0A0L103_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G002550 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 1.4e-78
Identity = 148/152 (97.37%), Postives = 150/152 (98.68%), Query Frame = 1


Query: 29  SERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGL 88
           +ERL FSIRVLLESAIHNCDEFQVKFKDVEKILDWE TCPRQVEIPFKPCQSPASDFTGL
Sbjct: 18  AERLTFSIRVLLESAIHNCDEFQVKFKDVEKILDWEKTCPRQVEIPFKPCQSPASDFTGL 77

Query: 89  PAVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDH 148
           PAVIDLACMKDA+NNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDH
Sbjct: 78  PAVIDLACMKDAVNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDH 137

Query: 149 SVEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           SVEVDMARSEKAVEANMELDFHRNKERFAFLK
Sbjct: 138 SVEVDMARSEKAVEANMELDFHRNKERFAFLK 169

[Close]

BLAST of CSPI03G00510 vs. TrEMBL
Match: V4MAY0_EUTSA (Aconitate hydratase OS=Eutrema salsugineum GN=EUTSA_v10024358mg PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-47
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCDEFQVK KDVEKILDWENT P+QVEIPFKP +    DFTG+P
Sbjct: 37  DKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 157 VQVDVARSENAVQANMELEFQRNKERFAFLK 162

[Close]

BLAST of CSPI03G00510 vs. TrEMBL
Match: M4D585_BRARP (Aconitate hydratase OS=Brassica rapa subsp. pekinensis PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-47
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCDEFQVK KDVEKILDWENT P+QVEIPFKP +    DFTG+P
Sbjct: 37  DKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 157 VQVDVARSENAVQANMELEFQRNKERFAFLK 162

[Close]

BLAST of CSPI03G00510 vs. TrEMBL
Match: A0A078BYY1_BRANA (Aconitate hydratase OS=Brassica napus GN=BnaC01g02790D PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-47
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCDEFQVK KDVEKILDWENT P+QVEIPFKP +    DFTG+P
Sbjct: 40  DKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVP 99

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 100 AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 159

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 160 VQVDVARSENAVQANMELEFQRNKERFAFLK 165

[Close]

BLAST of CSPI03G00510 vs. TrEMBL
Match: R0FAU4_9BRAS (Aconitate hydratase OS=Capsella rubella GN=CARUB_v10007484mg PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-47
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCDEFQVK KDVEKILDWENT P+QVEIPFKP +    DFTG+P
Sbjct: 37  DKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 157 VQVDVARSENAVQANMELEFQRNKERFAFLK 162

[Close]

BLAST of CSPI03G00510 vs. TAIR10
Match: AT4G35830.1 (AT4G35830.1 aconitase 1)

HSP 1 Score: 197.2 bits (500), Expect = 8.7e-51
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCDEFQVK KDVEKILDWENT P+QVEIPFKP +    DFTG+P
Sbjct: 37  DKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 157 VQVDVARSENAVQANMELEFQRNKERFAFLK 162

[Close]

BLAST of CSPI03G00510 vs. TAIR10
Match: AT2G05710.1 (AT2G05710.1 aconitase 3)

HSP 1 Score: 183.3 bits (464), Expect = 1.3e-46
Identity = 91/151 (60.26%), Postives = 110/151 (72.85%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCD FQV  +DVEKI+DWE T P+QVEIPFKP +    DFTG+P
Sbjct: 129 DKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSPKQVEIPFKPARVLLQDFTGVP 188

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+N +G +SNK+ PLV                         PVDLVIDHS
Sbjct: 189 AVVDLACMRDAMNKLGSDSNKINPLV-------------------------PVDLVIDHS 248

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 249 VQVDVARSENAVQANMELEFQRNKERFAFLK 254

[Close]

BLAST of CSPI03G00510 vs. TAIR10
Match: AT4G26970.1 (AT4G26970.1 aconitase 2)

HSP 1 Score: 160.2 bits (404), Expect = 1.2e-39
Identity = 79/151 (52.32%), Postives = 101/151 (66.89%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LPFS+R+LLESAI NCD +QV   DVEKILDWENT  +QVEI FKP +    DFTG+P
Sbjct: 134 DKLPFSVRILLESAIRNCDNYQVTKDDVEKILDWENTSTKQVEIAFKPARVILQDFTGVP 193

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
            ++DLA M+DA+ N+G + +K+ PLV                         PVDLV+DHS
Sbjct: 194 VLVDLASMRDAVKNLGSDPSKINPLV-------------------------PVDLVVDHS 253

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           ++VD ARSE A + N+EL+F RNKERF FLK
Sbjct: 254 IQVDFARSEDAAQKNLELEFKRNKERFTFLK 259

[Close]

BLAST of CSPI03G00510 vs. NCBI nr
Match: gi|700200502|gb|KGN55635.1| (hypothetical protein Csa_3G002550 [Cucumis sativus])

HSP 1 Score: 300.4 bits (768), Expect = 2.1e-78
Identity = 148/152 (97.37%), Postives = 150/152 (98.68%), Query Frame = 1


Query: 29  SERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGL 88
           +ERL FSIRVLLESAIHNCDEFQVKFKDVEKILDWE TCPRQVEIPFKPCQSPASDFTGL
Sbjct: 18  AERLTFSIRVLLESAIHNCDEFQVKFKDVEKILDWEKTCPRQVEIPFKPCQSPASDFTGL 77

Query: 89  PAVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDH 148
           PAVIDLACMKDA+NNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDH
Sbjct: 78  PAVIDLACMKDAVNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDH 137

Query: 149 SVEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           SVEVDMARSEKAVEANMELDFHRNKERFAFLK
Sbjct: 138 SVEVDMARSEKAVEANMELDFHRNKERFAFLK 169

[Close]

BLAST of CSPI03G00510 vs. NCBI nr
Match: gi|1009144375|ref|XP_015889766.1| (PREDICTED: aconitate hydratase 1 [Ziziphus jujuba])

HSP 1 Score: 199.9 bits (507), Expect = 3.8e-48
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           +RLP+SIR+LLESAI NCDEFQVK KDVEKI+DWENT P+QVEIPFKP +    DFTG+P
Sbjct: 37  DRLPYSIRILLESAIRNCDEFQVKIKDVEKIIDWENTSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+FHRNKERF FLK
Sbjct: 157 VQVDVARSENAVQANMELEFHRNKERFGFLK 162

[Close]

BLAST of CSPI03G00510 vs. NCBI nr
Match: gi|1009174474|ref|XP_015868363.1| (PREDICTED: aconitate hydratase 1 [Ziziphus jujuba])

HSP 1 Score: 199.9 bits (507), Expect = 3.8e-48
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           +RLP+SIR+LLESAI NCDEFQVK KDVEKI+DWENT P+QVEIPFKP +    DFTG+P
Sbjct: 37  DRLPYSIRILLESAIRNCDEFQVKIKDVEKIIDWENTSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+FHRNKERF FLK
Sbjct: 157 VQVDVARSENAVQANMELEFHRNKERFGFLK 162

[Close]

BLAST of CSPI03G00510 vs. NCBI nr
Match: gi|15233349|ref|NP_195308.1| (aconitate hydratase 1 [Arabidopsis thaliana])

HSP 1 Score: 197.2 bits (500), Expect = 2.5e-47
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCDEFQVK KDVEKILDWENT P+QVEIPFKP +    DFTG+P
Sbjct: 37  DKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVP 96

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 97  AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 156

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 157 VQVDVARSENAVQANMELEFQRNKERFAFLK 162

[Close]

BLAST of CSPI03G00510 vs. NCBI nr
Match: gi|674964712|emb|CDX69174.1| (BnaC01g02790D [Brassica napus])

HSP 1 Score: 197.2 bits (500), Expect = 2.5e-47
Identity = 98/151 (64.90%), Postives = 115/151 (76.16%), Query Frame = 1


Query: 30  ERLPFSIRVLLESAIHNCDEFQVKFKDVEKILDWENTCPRQVEIPFKPCQSPASDFTGLP 89
           ++LP+SIR+LLESAI NCDEFQVK KDVEKILDWENT P+QVEIPFKP +    DFTG+P
Sbjct: 40  DKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLLQDFTGVP 99

Query: 90  AVIDLACMKDALNNIGGNSNKVIPLVGEKLTSKLRSQKFGSSSKNKPSVNIPVDLVIDHS 149
           AV+DLACM+DA+NN+GG+SNK+ PLV                         PVDLVIDHS
Sbjct: 100 AVVDLACMRDAMNNLGGDSNKINPLV-------------------------PVDLVIDHS 159

Query: 150 VEVDMARSEKAVEANMELDFHRNKERFAFLK 181
           V+VD+ARSE AV+ANMEL+F RNKERFAFLK
Sbjct: 160 VQVDVARSENAVQANMELEFQRNKERFAFLK 165

The following BLAST results are available for this feature:

BLAST of CSPI03G00510 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ACO1_ARATH	1.5e-49	64.90	Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2	[more]
ACOC_CUCMA	1.2e-46	61.59	Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1	[more]
ACO2M_ARATH	2.3e-45	60.26	Aconitate hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=ACO2 PE=1 SV=2	[more]
ACOC_ORYSJ	2.9e-40	54.97	Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os0...	[more]
ACO3M_ARATH	2.1e-38	52.32	Aconitate hydratase 3, mitochondrial OS=Arabidopsis thaliana GN=ACO3 PE=2 SV=3	[more]

BLAST of CSPI03G00510 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L103_CUCSA	1.4e-78	97.37	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G002550 PE=4 SV=1	[more]
V4MAY0_EUTSA	1.7e-47	64.90	Aconitate hydratase OS=Eutrema salsugineum GN=EUTSA_v10024358mg PE=3 SV=1	[more]
M4D585_BRARP	1.7e-47	64.90	Aconitate hydratase OS=Brassica rapa subsp. pekinensis PE=3 SV=1	[more]
A0A078BYY1_BRANA	1.7e-47	64.90	Aconitate hydratase OS=Brassica napus GN=BnaC01g02790D PE=3 SV=1	[more]
R0FAU4_9BRAS	1.7e-47	64.90	Aconitate hydratase OS=Capsella rubella GN=CARUB_v10007484mg PE=3 SV=1	[more]

BLAST of CSPI03G00510 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G35830.1	8.7e-51	64.90	aconitase 1	[more]
AT2G05710.1	1.3e-46	60.26	aconitase 3	[more]
AT4G26970.1	1.2e-39	52.32	aconitase 2	[more]

BLAST of CSPI03G00510 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700200502\|gb\|KGN55635.1\|	2.1e-78	97.37	hypothetical protein Csa_3G002550 [Cucumis sativus]	[more]
gi\|1009144375\|ref\|XP_015889766.1\|	3.8e-48	64.90	PREDICTED: aconitate hydratase 1 [Ziziphus jujuba]	[more]
gi\|1009174474\|ref\|XP_015868363.1\|	3.8e-48	64.90	PREDICTED: aconitate hydratase 1 [Ziziphus jujuba]	[more]
gi\|15233349\|ref\|NP_195308.1\|	2.5e-47	64.90	aconitate hydratase 1 [Arabidopsis thaliana]	[more]
gi\|674964712\|emb\|CDX69174.1\|	2.5e-47	64.90	BnaC01g02790D [Brassica napus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001030	Acoase/IPM_deHydtase_lsu_aba
IPR015931	Acnase/IPM_dHydase_lsu_aba_1/3
IPR015937	Aconitase/isopropylmalate dehydratase

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0072350	tricarboxylic acid metabolic process
biological_process	GO:0008152	metabolic process
cellular_component	GO:0044444	cytoplasmic part
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0016829	lyase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G00510.1	CSPI03G00510.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001030	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain	unknown	SSF53732	Aconitase iron-sulfur domain	coord: 27..179 score: 9.55
IPR015931	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3	GENE3D	G3DSA:3.30.499.10		coord: 29..99 score: 1.1E-24coord: 136..180 score: 1.1
IPR015937	Aconitase/isopropylmalate dehydratase	PANTHER	PTHR11670	ACONITASE	coord: 141..180 score: 3.3E-65coord: 30..115 score: 3.3
None	No IPR available	PANTHER	PTHR11670:SF40	ACONITATE HYDRATASE 1	coord: 141..180 score: 3.3E-65coord: 30..115 score: 3.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G00510	Csa3G002550	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G00510	Lsi07G010800	Bottle gourd (USVL1VR-Ls)	cpilsiB227
CSPI03G00510	MELO3C032251.2	Melon (DHL92) v3.6.1	cpimedB225
CSPI03G00510	CsaV3_3G000530	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G00510	Bhi11G000246	Wax gourd	cpiwgoB226

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G00510	Cucumber (Chinese Long) v3	cpicucB184
CSPI03G00510	Watermelon (97103) v2	cpiwmbB182
CSPI03G00510	Watermelon (97103) v2	cpiwmbB184
CSPI03G00510	Wax gourd	cpiwgoB329
CSPI03G00510	Wild cucumber (PI 183967)	cpicpiB133
CSPI03G00510	Cucumber (Gy14) v1	cgycpiB025
CSPI03G00510	Cucumber (Gy14) v1	cgycpiB484
CSPI03G00510	Cucurbita maxima (Rimu)	cmacpiB332
CSPI03G00510	Cucurbita maxima (Rimu)	cmacpiB666
CSPI03G00510	Cucurbita maxima (Rimu)	cmacpiB840
CSPI03G00510	Cucurbita maxima (Rimu)	cmacpiB871
CSPI03G00510	Cucurbita moschata (Rifu)	cmocpiB323
CSPI03G00510	Cucurbita moschata (Rifu)	cmocpiB658
CSPI03G00510	Cucurbita moschata (Rifu)	cmocpiB825
CSPI03G00510	Cucurbita moschata (Rifu)	cmocpiB852
CSPI03G00510	Cucumber (Chinese Long) v2	cpicuB156
CSPI03G00510	Melon (DHL92) v3.5.1	cpimeB240
CSPI03G00510	Melon (DHL92) v3.5.1	cpimeB258
CSPI03G00510	Watermelon (Charleston Gray)	cpiwcgB190
CSPI03G00510	Watermelon (Charleston Gray)	cpiwcgB212
CSPI03G00510	Watermelon (97103) v1	cpiwmB200
CSPI03G00510	Watermelon (97103) v1	cpiwmB262
CSPI03G00510	Cucurbita pepo (Zucchini)	cpecpiB064
CSPI03G00510	Cucurbita pepo (Zucchini)	cpecpiB215
CSPI03G00510	Cucurbita pepo (Zucchini)	cpecpiB485
CSPI03G00510	Cucurbita pepo (Zucchini)	cpecpiB557
CSPI03G00510	Cucurbita pepo (Zucchini)	cpecpiB853
CSPI03G00510	Bottle gourd (USVL1VR-Ls)	cpilsiB164
CSPI03G00510	Melon (DHL92) v3.6.1	cpimedB254
CSPI03G00510	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G00510	Cucumber (Gy14) v2	cgybcpiB272
CSPI03G00510	Silver-seed gourd	carcpiB0179
CSPI03G00510	Silver-seed gourd	carcpiB0441
CSPI03G00510	Silver-seed gourd	carcpiB0467
CSPI03G00510	Silver-seed gourd	carcpiB0671