CSPI02G23290 (gene) Wild cucumber (PI 183967)

NameCSPI02G23290
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionPlant protein of unknown function (DUF247)
LocationChr2 : 20159507 .. 20161174 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAATAGTGAGAATAACGATGATCAAGTTTCAAATGAAGCAGTGATTAGTGAGGTTAAACGAAAGCATTGTGGTGATATTGTGATATCAATAAAGAGCGAGTTGGAACAACTACCTGTTGTTAACACAAAATGCAGCATTTATAGAGTTCCCAAATTGCTATGCCAAATGAATAAAACAGCCTATGTCCCTCAAGTCATTTCCATCGGCCCTTTTCATCATGGTTTTAAGGATTTGAATGCTACAGAACGTTATAAGCTTCAAGGTCTTCGTAACTTTCTACATCGTTTCGATGATCGCAAGGAGAAATCATTGAAGGAGCTTGTAAAAATTGCTCAATCTTGGGTAGAAGAAGCCCGTAGTTGTTATGCTGATCCGATAGGCATGGACGACCACGAGTTTGTTAAGATGATGCTTGTGGATGGTTGTTTCATAGTAGAGTTCTTTATACTTGTTCACAACCAATACACTACCTCAGATGATCATAAGTTCCCTCAAATTGATCCAAATGTCGACCTTTTATTTTATCAAGGAATAATTCCTGATGTATATTTTGACTTGATAAAGCTCGAAAATCAACTTCCTTTCTTCCTTCTTCAACGTTTGTTTGAGCTAATACCAAAAGAAAACAACCCCAAATCTCTAATCCCAAATAATGAATTTTCCACCATCTCCTTTCTAGAACTTACATTTGAGTTTCTTCGTCTTGGGTGGGTAGAAAATTATGTACAACTTGGTGAAAGAATTGTTTGGTCTGAGTTTGACCCCGAACACTTGGTGGATTTTTTAAGGCTATACTACATCTCAAGTTCAGATGAAGAATGCCACGACAACATGAAGATTAATATTTTAAGCTTCTTCTACGTGTGTCGTCGATTTTGGAACAAGACTAAAAAGGAAACAGGTGAGAAGAAAAAGGTTCATGCGGAAAACAGAGAAAAACAAAGGACTCTTTCAATCTTCAAACGAACATACTCGCCAGAATATTGGACGAAGGCGAAGAATAATTTGTTGAGTAGAACAGGATCATGTTTTTGTCGAAACAAGAAGAAACATGAGGAAAATGACCATGAAGAAAGTTACCATGAGGAAAAGATGTGGATTCCTCCATCCATAACTGAGCTCTCTGAGGCTGGTGTCACCGTCAAGAAGGCAAAAAAGTCTCCATTTATAACCAACATAACCTTCAAAAATGGGGTTATGGAAATTCCACCTATAGTTATTGACGATTACTTCGAAACCATTACTCGAAACTTGATAGCGTATGAGCATCTAGACGCAAAAAATTATCAAGAAATGAATGTGATCTCATATATCTTGTTTATGGATTATTTGATAAGTACAGAGAAAGACGTGAGTTTACTTGAGAATGCAGGAATCATAGTCAACGATATCGGTGGCAGCAATAAAGAAGTTTCAGAATTGTTTAACAATCTCAGTAAATTTGTCAATAGGTCAGGTGATATTAGTGCATATTTTGAGAAATTCAATGGTATCAGCAAAGCTTTATGTGAGTATCGTGACAGACGATGGAACAAGTTGAAAGCTTCATTAAAACATAATTATTTCAATACGCCATGGGCTGGTATCTCCGTCTTTGCTGCAACCTTCATTATTGTTCTCACTCTCCTTCAAACAATATTTGCGGGTATATCAACTTTCTAG

mRNA sequence

ATGGAAAATAGTGAGAATAACGATGATCAAGTTTCAAATGAAGCAGTGATTAGTGAGGTTAAACGAAAGCATTGTGGTGATATTGTGATATCAATAAAGAGCGAGTTGGAACAACTACCTGTTGTTAACACAAAATGCAGCATTTATAGAGTTCCCAAATTGCTATGCCAAATGAATAAAACAGCCTATGTCCCTCAAGTCATTTCCATCGGCCCTTTTCATCATGGTTTTAAGGATTTGAATGCTACAGAACGTTATAAGCTTCAAGGTCTTCGTAACTTTCTACATCGTTTCGATGATCGCAAGGAGAAATCATTGAAGGAGCTTGTAAAAATTGCTCAATCTTGGGTAGAAGAAGCCCGTAGTTGTTATGCTGATCCGATAGGCATGGACGACCACGAGTTTGTTAAGATGATGCTTGTGGATGGTTGTTTCATAGTAGAGTTCTTTATACTTGTTCACAACCAATACACTACCTCAGATGATCATAAGTTCCCTCAAATTGATCCAAATGTCGACCTTTTATTTTATCAAGGAATAATTCCTGATGTATATTTTGACTTGATAAAGCTCGAAAATCAACTTCCTTTCTTCCTTCTTCAACGTTTGTTTGAGCTAATACCAAAAGAAAACAACCCCAAATCTCTAATCCCAAATAATGAATTTTCCACCATCTCCTTTCTAGAACTTACATTTGAGTTTCTTCGTCTTGGGTGGGTAGAAAATTATGTACAACTTGGTGAAAGAATTGTTTGGTCTGAGTTTGACCCCGAACACTTGGTGGATTTTTTAAGGCTATACTACATCTCAAGTTCAGATGAAGAATGCCACGACAACATGAAGATTAATATTTTAAGCTTCTTCTACGTGTGTCGTCGATTTTGGAACAAGACTAAAAAGGAAACAGGTGAGAAGAAAAAGGTTCATGCGGAAAACAGAGAAAAACAAAGGACTCTTTCAATCTTCAAACGAACATACTCGCCAGAATATTGGACGAAGGCGAAGAATAATTTGTTGAGTAGAACAGGATCATGTTTTTGTCGAAACAAGAAGAAACATGAGGAAAATGACCATGAAGAAAGTTACCATGAGGAAAAGATGTGGATTCCTCCATCCATAACTGAGCTCTCTGAGGCTGGTGTCACCGTCAAGAAGGCAAAAAAGTCTCCATTTATAACCAACATAACCTTCAAAAATGGGGTTATGGAAATTCCACCTATAGTTATTGACGATTACTTCGAAACCATTACTCGAAACTTGATAGCGTATGAGCATCTAGACGCAAAAAATTATCAAGAAATGAATGTGATCTCATATATCTTGTTTATGGATTATTTGATAAGTACAGAGAAAGACGTGAGTTTACTTGAGAATGCAGGAATCATAGTCAACGATATCGGTGGCAGCAATAAAGAAGTTTCAGAATTGTTTAACAATCTCAGTAAATTTGTCAATAGGTCAGGTGATATTAGTGCATATTTTGAGAAATTCAATGGTATCAGCAAAGCTTTATGTGAGTATCGTGACAGACGATGGAACAAGTTGAAAGCTTCATTAAAACATAATTATTTCAATACGCCATGGGCTGGTATCTCCGTCTTTGCTGCAACCTTCATTATTGTTCTCACTCTCCTTCAAACAATATTTGCGGGTATATCAACTTTCTAG

Coding sequence (CDS)

ATGGAAAATAGTGAGAATAACGATGATCAAGTTTCAAATGAAGCAGTGATTAGTGAGGTTAAACGAAAGCATTGTGGTGATATTGTGATATCAATAAAGAGCGAGTTGGAACAACTACCTGTTGTTAACACAAAATGCAGCATTTATAGAGTTCCCAAATTGCTATGCCAAATGAATAAAACAGCCTATGTCCCTCAAGTCATTTCCATCGGCCCTTTTCATCATGGTTTTAAGGATTTGAATGCTACAGAACGTTATAAGCTTCAAGGTCTTCGTAACTTTCTACATCGTTTCGATGATCGCAAGGAGAAATCATTGAAGGAGCTTGTAAAAATTGCTCAATCTTGGGTAGAAGAAGCCCGTAGTTGTTATGCTGATCCGATAGGCATGGACGACCACGAGTTTGTTAAGATGATGCTTGTGGATGGTTGTTTCATAGTAGAGTTCTTTATACTTGTTCACAACCAATACACTACCTCAGATGATCATAAGTTCCCTCAAATTGATCCAAATGTCGACCTTTTATTTTATCAAGGAATAATTCCTGATGTATATTTTGACTTGATAAAGCTCGAAAATCAACTTCCTTTCTTCCTTCTTCAACGTTTGTTTGAGCTAATACCAAAAGAAAACAACCCCAAATCTCTAATCCCAAATAATGAATTTTCCACCATCTCCTTTCTAGAACTTACATTTGAGTTTCTTCGTCTTGGGTGGGTAGAAAATTATGTACAACTTGGTGAAAGAATTGTTTGGTCTGAGTTTGACCCCGAACACTTGGTGGATTTTTTAAGGCTATACTACATCTCAAGTTCAGATGAAGAATGCCACGACAACATGAAGATTAATATTTTAAGCTTCTTCTACGTGTGTCGTCGATTTTGGAACAAGACTAAAAAGGAAACAGGTGAGAAGAAAAAGGTTCATGCGGAAAACAGAGAAAAACAAAGGACTCTTTCAATCTTCAAACGAACATACTCGCCAGAATATTGGACGAAGGCGAAGAATAATTTGTTGAGTAGAACAGGATCATGTTTTTGTCGAAACAAGAAGAAACATGAGGAAAATGACCATGAAGAAAGTTACCATGAGGAAAAGATGTGGATTCCTCCATCCATAACTGAGCTCTCTGAGGCTGGTGTCACCGTCAAGAAGGCAAAAAAGTCTCCATTTATAACCAACATAACCTTCAAAAATGGGGTTATGGAAATTCCACCTATAGTTATTGACGATTACTTCGAAACCATTACTCGAAACTTGATAGCGTATGAGCATCTAGACGCAAAAAATTATCAAGAAATGAATGTGATCTCATATATCTTGTTTATGGATTATTTGATAAGTACAGAGAAAGACGTGAGTTTACTTGAGAATGCAGGAATCATAGTCAACGATATCGGTGGCAGCAATAAAGAAGTTTCAGAATTGTTTAACAATCTCAGTAAATTTGTCAATAGGTCAGGTGATATTAGTGCATATTTTGAGAAATTCAATGGTATCAGCAAAGCTTTATGTGAGTATCGTGACAGACGATGGAACAAGTTGAAAGCTTCATTAAAACATAATTATTTCAATACGCCATGGGCTGGTATCTCCGTCTTTGCTGCAACCTTCATTATTGTTCTCACTCTCCTTCAAACAATATTTGCGGGTATATCAACTTTCTAG
BLAST of CSPI02G23290 vs. Swiss-Prot
Match: Y3720_ARATH (UPF0481 protein At3g47200 OS=Arabidopsis thaliana GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.5e-18
Identity = 55/161 (34.16%), Postives = 92/161 (57.14%), Query Frame = 1

Query: 46  CSIYRVPKLLCQMNKTAYVPQVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRKEKS 105
           C I+RVP+    +N  AY P+V+SIGP+H+G K L   +++K + L+ FL   D+ K+K 
Sbjct: 46  CCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFL---DEAKKKD 105

Query: 106 LKE--LVKIAQSWVEEARSCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSDDH 165
           ++E  LVK      ++ R  Y++ +    H+ + MM++DGCFI+  F+++      S+D 
Sbjct: 106 VEENVLVKAVVDLEDKIRKSYSEEL-KTGHDLMFMMVLDGCFILMVFLIMSGNIELSEDP 165

Query: 166 KFPQIDPNVDLLFYQGIIPDVYFDLIKLENQLPFFLLQRLF 205
            F              ++  +  DL+ LENQ+PFF+LQ L+
Sbjct: 166 IFS----------IPWLLSSIQSDLLLLENQVPFFVLQTLY 192

BLAST of CSPI02G23290 vs. Swiss-Prot
Match: Y3264_ARATH (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.4e-13
Identity = 49/175 (28.00%), Postives = 91/175 (52.00%), Query Frame = 1

Query: 31  SIKSELEQLPVVNTKCSIYRVPKLLCQMNKTAYVPQVISIGPFHHGFKDLNATERYKLQG 90
           S+ +ELE+  +     SI+ VPK L   +  +Y P  +SIGP+H    +L+  ERYKL  
Sbjct: 28  SLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMI 87

Query: 91  LRNFLHRFDDRKEKSLKELVKIAQSWVEEARSCYADPIGMDDHEFVKMMLVDGCFIVEFF 150
            R   ++++  +     +LV+  QS   + R+CY   IG +    + +M VD  F++EF 
Sbjct: 88  ARKIRNQYNSFR---FHDLVEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFLIEFL 147

Query: 151 ILVHNQYTTSDDHKFPQIDPNVDLLFYQGIIPDVYFDLIKLENQLPFFLLQRLFE 206
            +          + F +++  ++ + +  I+ D+    + +ENQ+P F+L++  E
Sbjct: 148 KI----------YSFRKVETLINRVGHNEILRDI----MMIENQIPLFVLRKTLE 185

BLAST of CSPI02G23290 vs. TrEMBL
Match: A0A0A0LQ64_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G381630 PE=4 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 5.7e-203
Identity = 406/568 (71.48%), Postives = 441/568 (77.64%), Query Frame = 1

Query: 1   MENSENNDDQVSNEAVISEVKRKHCGDIVISIKSELEQLPVVNTKCSIYRVPKLLCQMNK 60
           ME  ENND                C DIVISIKS+L+QL VVNT+C+IYRVPKLLCQMN 
Sbjct: 1   MEVGENND----------------CRDIVISIKSKLKQLHVVNTECNIYRVPKLLCQMNM 60

Query: 61  TAYVPQVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRK-EKSLKELVKIAQSWVEE 120
           TAYVPQVISIGP HHG KDLNATE YKLQGLRNFL RFDD   EK L+ LV+I Q +V  
Sbjct: 61  TAYVPQVISIGPLHHGSKDLNATECYKLQGLRNFLRRFDDSYGEKLLENLVEITQFYVGR 120

Query: 121 ARSCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSDDHKFPQIDPNVDLLFY-Q 180
           ARSCYADPI MDDHEFVKMMLVDGCFIVE FILVHN  TTSDDHKF +ID NVDLLFY Q
Sbjct: 121 ARSCYADPIDMDDHEFVKMMLVDGCFIVELFILVHN-ITTSDDHKFSKIDQNVDLLFYYQ 180

Query: 181 GIIPDVYFDLIKLENQLPFFLLQRLFELIPKENNP-KSLIPNNEFST-------ISFLEL 240
           GIIPDVYFDLIKLENQLPFF+LQ LF+LIP++NN  KSLI N +          I FLEL
Sbjct: 181 GIIPDVYFDLIKLENQLPFFILQLLFDLIPRDNNQSKSLILNRKSPILDKNSLFIPFLEL 240

Query: 241 TFEFLRLGWVENYVQLGER-IVWSEFDPEHLVDFLRLYY--ISSSDEECHDNMKINILSF 300
           TFE LRLGWV++YVQLG+R I   EF P+HL+DFL LYY   SS+D+ECH+         
Sbjct: 241 TFENLRLGWVKDYVQLGKRSITLHEFYPKHLLDFLTLYYENSSSNDDECHN--------- 300

Query: 301 FYVCRRFWNKTKKETGEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFC 360
                        ET EKKKVHAENR+ Q+TLSIFK+TY      K KNNL S    CF 
Sbjct: 301 -------------ETSEKKKVHAENRDNQKTLSIFKQTY-----LKVKNNLCS----CFR 360

Query: 361 RNKKKHEENDHEESYHEEKMWIPPSITELSEAGVTVKKAKKSPFITNITFKNGVMEIPPI 420
           RNKK H ENDHE+     KM+IPPSITELSEAGVT KK KK P ITNITFKNGV+EIPPI
Sbjct: 361 RNKK-HGENDHEK-----KMYIPPSITELSEAGVTFKKPKKLPIITNITFKNGVLEIPPI 420

Query: 421 VIDDYFETITRNLIAYEHLDAKNYQEMNVISYILFMDYLISTEKDVSLLENAGIIVNDIG 480
           VID YFETITRNLIAYEH+D K Y+EM VISYILFMDYLISTEKDVSLLE AGIIVNDIG
Sbjct: 421 VIDHYFETITRNLIAYEHMD-KIYKEMKVISYILFMDYLISTEKDVSLLEKAGIIVNDIG 480

Query: 481 GSNKEVSELFNNLSKFVNRSGDISAYFEKFNGISKALCEYRDRRWNKLKASLKHNYFNTP 540
           GS+KEVS+LFNNLSKFVNRS + S YF+KF GISKALCE+RDRRWNKLKASLKHNYFNTP
Sbjct: 481 GSHKEVSKLFNNLSKFVNRSDNNSEYFKKFQGISKALCEHRDRRWNKLKASLKHNYFNTP 513

Query: 541 WAGISVFAATFIIVLTLLQTIFAGISTF 556
           WA ISVFAAT +IVLTLLQTIFA ISTF
Sbjct: 541 WASISVFAATLVIVLTLLQTIFAVISTF 513

BLAST of CSPI02G23290 vs. TrEMBL
Match: A0A0A0LQ49_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380070 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 3.7e-117
Identity = 230/333 (69.07%), Postives = 246/333 (73.87%), Query Frame = 1

Query: 63  YVPQVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRKEKSLKELVKIAQ-SWVEEAR 122
           YVPQVISIGPFHHG KDLN TERYK QGLRNFL R +D KEKSL+EL KIAQ SWVEEA 
Sbjct: 19  YVPQVISIGPFHHGSKDLNVTERYKFQGLRNFLRRLNDHKEKSLEELTKIAQLSWVEEAC 78

Query: 123 SCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSDDHKFPQIDPNVDLLFYQGII 182
            CY  PI MD                              DH+F      V ++   GII
Sbjct: 79  GCYIYPIDMD------------------------------DHEF------VKMMCVDGII 138

Query: 183 PDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIPNNEFSTISFLELTFEFLRLGWVE 242
           PDVYFDLIKLENQLPFFLLQRLFELIPKENN KSLIPNNEFSTISFLELTF+FL LG  E
Sbjct: 139 PDVYFDLIKLENQLPFFLLQRLFELIPKENNLKSLIPNNEFSTISFLELTFQFLHLGCAE 198

Query: 243 NYVQLGERIVWSEFDPEHLVDFLRLYYISSSDEECHDNMKINILSFFYVCRRFWNKTKKE 302
           NYVQLGERIVWS+FDP+HLVDFL+LYYISSS +EC ++ KI ILSF Y C R+ N T KE
Sbjct: 199 NYVQLGERIVWSKFDPKHLVDFLKLYYISSSADECREDKKIRILSFSYSCCRYLNMTNKE 258

Query: 303 TGEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFCRNKKKHEENDHEES 362
           T EKKKVHAEN EKQRT SIFKRTYSP+YWTK KNNLLSR G CF RNK KHEEN     
Sbjct: 259 TSEKKKVHAENGEKQRTHSIFKRTYSPKYWTKGKNNLLSRAGLCFGRNKTKHEEN----- 305

Query: 363 YHEEKMWIPPSITELSEAGVTVKKAKKSPFITN 395
                MWIPPSITE+S+ GV VKKAKKSPFITN
Sbjct: 319 -----MWIPPSITEISKVGVIVKKAKKSPFITN 305

BLAST of CSPI02G23290 vs. TrEMBL
Match: A0A0A0K4A0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G068570 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 4.5e-99
Identity = 181/218 (83.03%), Postives = 197/218 (90.37%), Query Frame = 1

Query: 1   MENSENNDDQVSNEAVISEVKRKHCGDIVISIKSELEQLPVVNTKCSIYRVPKLLCQMNK 60
           M+N  NND QVSNE VISE+K  H GD VISIKS+LEQLPVVN +C IYRVPKLLCQM K
Sbjct: 1   MKNGANND-QVSNEVVISEIKPNHWGDTVISIKSKLEQLPVVNKECKIYRVPKLLCQMKK 60

Query: 61  TAYVPQVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRKEKSLKELVKIAQSWVEEA 120
           T YVPQVISIGPFHHG KDLNATE YKLQGLRNFL R D+ KEKSL+ELVKIA+SW+EEA
Sbjct: 61  TTYVPQVISIGPFHHGSKDLNATEPYKLQGLRNFLRRLDNHKEKSLEELVKIARSWIEEA 120

Query: 121 RSCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSDDHKFPQIDPNVDLLFYQGI 180
           RSCY DPI MDD+EFVKMMLVDGCFIVEFFILV++QYTTS+DHKFPQIDPN  LLFYQGI
Sbjct: 121 RSCYVDPIDMDDYEFVKMMLVDGCFIVEFFILVYSQYTTSNDHKFPQIDPNAGLLFYQGI 180

Query: 181 IPDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIP 219
           IPD+YFDL+KLENQLPFF+LQRLFEL+PKENNPKSLIP
Sbjct: 181 IPDMYFDLLKLENQLPFFILQRLFELMPKENNPKSLIP 217

BLAST of CSPI02G23290 vs. TrEMBL
Match: A0A0A0K2S5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G068560 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 5.7e-86
Identity = 184/251 (73.31%), Postives = 198/251 (78.88%), Query Frame = 1

Query: 308 VHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFCRNKKKHEENDHEESYHEEKM 367
           VHAE++EKQRTLSIFKRTYSPEY TK KNNLLSRT SCF R KKKHE+ND EE     K+
Sbjct: 2   VHAESKEKQRTLSIFKRTYSPEYCTKEKNNLLSRTDSCFYRKKKKHEQNDPEE-----KI 61

Query: 368 WIPPSITELSEAGVTVKKAKKSPFITNITFKNGVMEIPPIVIDDYFETITRNLIAYEHLD 427
           WIPPSITEL EAGVTVKKAKKSPFITNIT+KNG                        HL 
Sbjct: 62  WIPPSITELFEAGVTVKKAKKSPFITNITYKNG------------------------HLA 121

Query: 428 AKNYQEMNVISYILFMDYLISTEKDVSLLENAGIIVNDIGGSNKEVSELFNNLSKFVNRS 487
           AK+YQEMNVISYILFMD LIST+KDV LLE AGIIVNDI GS+KEVSELFNNLSKFV RS
Sbjct: 122 AKSYQEMNVISYILFMDCLISTKKDVRLLEKAGIIVNDIDGSDKEVSELFNNLSKFVRRS 181

Query: 488 GDISAYFEK-FNGISKALCE-YRDRRWNKLKA-SLKHNYFNTPWAGISVFAATFIIVLTL 547
            DISAYFEK FN ISKALCE  R+R+W KLK  SLKHNYFNTPWAGI++F  TFII+LTL
Sbjct: 182 SDISAYFEKYFNYISKALCENNRNRQWKKLKGRSLKHNYFNTPWAGIAIFVGTFIIILTL 223

Query: 548 LQTIFAGISTF 556
           LQTIF+ ISTF
Sbjct: 242 LQTIFSVISTF 223

BLAST of CSPI02G23290 vs. TrEMBL
Match: A0A0A0LPJ5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380630 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.7e-69
Identity = 183/434 (42.17%), Postives = 249/434 (57.37%), Query Frame = 1

Query: 130 MDDHEFVKMMLVDGCFIVEFFILV-----HNQYTTSDDHKFPQIDPNVDLLFYQGIIPDV 189
           M++  FV MMLVD CFI+EFFIL+     H +     DH F QI   VD  FYQG    +
Sbjct: 1   MEEKTFVLMMLVDACFILEFFILLIDPLGHGRNLFGVDHFF-QIQDIVDFSFYQGKFFQI 60

Query: 190 YFDLIKLENQLPFFLLQRLFELIPKENNPKSLIPNNEFSTISFLELTFEFLRLGWVENYV 249
             DLIKLENQ+PFFLLQ LF+LIPK   P      NE   IS +++T   L+ G+V  +V
Sbjct: 61  LVDLIKLENQVPFFLLQNLFDLIPKRAIPTG---ENEVGLISLIDITSMILK-GFV--FV 120

Query: 250 QLGERIVWSEFDPEHLVDFLRLYY--ISSSDEECHDNMKINILSFFYVCRRFWNKTKKET 309
           +  +        P+HL+DFL  Y+  ++S+D+   +          YV     +K     
Sbjct: 121 RKYKINDLDHKKPKHLLDFLGSYFFPLASNDDPTQNKQ--------YVITTISDK----- 180

Query: 310 GEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFCRNKKKHEENDHEESY 369
                       K   L  F R     +W K  N+L + +  C+  NKK     + E S 
Sbjct: 181 ------------KDNNLLRFIRLLFTAHWQKKNNDLFNVSAMCYSSNKKT--TTNEENSE 240

Query: 370 HEEKMWIPPSITELSEAGVTVKKAK-KSPFITNITFKNGVMEIPPIVIDDYFETITRNLI 429
           H  ++  PPS+TEL EAGV VK  K +     NI+F+NGV++IP + ID  FE + RN+I
Sbjct: 241 HHFRL-SPPSMTELCEAGVIVKAVKNEDTCFMNISFENGVLKIPCLEIDCTFEIVIRNVI 300

Query: 430 AYEHLDAKNYQEMNVISYILFMDYLISTEKDVSLLENAGIIVNDIGGSNKEVSELFNNLS 489
           A++   A N +    I Y+LF+D LI+TE+D  LL N G+I+N +GGS+K+++E+FNNLS
Sbjct: 301 AFDQYPAGN-KNAYAIHYVLFLDDLINTEQDAHLLANVGVIINTLGGSDKDITEMFNNLS 360

Query: 490 KFVNRSGDISAYFEKFNGISKALCEYRDRRWNKLKASLKHNYFNTPWAGISVFAATFIIV 549
           K+V            F+ ISK L ++ ++RWNK  ASLKHNYFNTPWA IS  AAT +I 
Sbjct: 361 KYVT-----FPVCSHFDDISKDLRKHCNKRWNKAIASLKHNYFNTPWAIISFLAATILIF 393

Query: 550 LTLLQTIFAGISTF 556
           LT+LQTIF+ ISTF
Sbjct: 421 LTILQTIFSAISTF 393

BLAST of CSPI02G23290 vs. TAIR10
Match: AT3G50170.1 (AT3G50170.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 206.1 bits (523), Expect = 5.7e-53
Identity = 170/557 (30.52%), Postives = 271/557 (48.65%), Query Frame = 1

Query: 12  SNEAVISEVKRKHCGDI-VISIKSELEQLP-----VVNTKCSIYRVPKLLCQMNKTAYVP 71
           S + V+ E   +  GD  VISI+ +LEQ        +  K  IYRVP  L + +K +Y P
Sbjct: 73  SGKEVVEERPEETTGDSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQENDKKSYFP 132

Query: 72  QVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRKEKSLKELVKIAQSWVEEARSCYA 131
           Q +S+GP+HHG K L   ER+K + L   L R   R E     + ++     E+AR+CY 
Sbjct: 133 QTVSLGPYHHGKKRLRPMERHKWRALNKVLKRLKQRIEMYTNAMRELE----EKARACYE 192

Query: 132 DPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSDDHKFPQIDPNVDLLFYQGIIPDVY 191
            PI +  +EF +M+++DGCF++E F      +T   +  + + DP   +   +G++  + 
Sbjct: 193 GPISLSRNEFTEMLVLDGCFVLELFRGTVEGFT---EIGYARNDP---VFAMRGLMHSIQ 252

Query: 192 FDLIKLENQLPFFLLQRLFELIPKENNPKSLIPNNEFSTISFLELTFEFLRLGWVENYVQ 251
            D+I LENQLP F+L RL EL         L   N+   ++ + +        + +  + 
Sbjct: 253 RDMIMLENQLPLFVLDRLLEL--------QLGTQNQTGIVAHVAVK-------FFDPLMP 312

Query: 252 LGERIVWSEFDPEHLVDFLRLYYISSSDEECHDNMKINILSFFYVCRRFWNKTKKETGEK 311
            GE +  ++ D   L+++L                                K+    G+K
Sbjct: 313 TGEAL--TKPDQSKLMNWLE-------------------------------KSLDTLGDK 372

Query: 312 KKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFCRNKKKHEENDHEESYHEE 371
            ++H         L +F+R+      T    +LL R      RN +  ++   +      
Sbjct: 373 GELHC--------LDVFRRSLLQSSPTPNTRSLLKR----LTRNTRVVDKRQQQ------ 432

Query: 372 KMWIPPSITELSEAGVTVKKAKKSPFITNITFKNGVMEIPPIVIDDYFETITRNLIAYE- 431
              +   +TEL EAGV  +K K   F  +I FKNG +EIP ++I D  +++  NLIA+E 
Sbjct: 433 ---LVHCVTELREAGVKFRKRKTDRF-WDIEFKNGYLEIPKLLIHDGTKSLFSNLIAFEQ 492

Query: 432 -HLDAKNYQEMNVISYILFMDYLISTEKDVSLLENAGIIVNDIGGSNKEVSELFNNLSKF 491
            H+++ N+    + SYI+FMD LI++ +DVS L   GII + +G S+ EV++LFN L + 
Sbjct: 493 CHIESSNH----ITSYIIFMDNLINSSEDVSYLHYCGIIEHWLG-SDSEVADLFNRLCQE 530

Query: 492 V----------NRSGDISAYFEKFNGISKALCEYRDRRWNKLKASLKHNYFNTPWAGISV 551
           V            SGD++ Y+              +R+WN LKA+L H YFN PWA  S 
Sbjct: 553 VVFDPKDSHLSRLSGDVNRYY--------------NRKWNVLKATLTHKYFNNPWAYFSF 530

BLAST of CSPI02G23290 vs. TAIR10
Match: AT3G44710.1 (AT3G44710.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 174.1 bits (440), Expect = 2.4e-43
Identity = 142/518 (27.41%), Postives = 234/518 (45.17%), Query Frame = 1

Query: 45  KCSIYRVP-KLLCQMNKTAYVPQVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRKE 104
           KC I+++  K   +  K AY P+V+S+GP+HHG K+L   E +KL+ L+ F+   D+ K 
Sbjct: 46  KCCIFKISHKPENKKYKAAYEPRVVSLGPYHHGKKNLQMIEEHKLRFLKIFM---DEAKR 105

Query: 105 KSLKE--LVKIAQSWVEEARSCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSD 164
           K +    L+K      E+ R  Y++ +  D  + ++MM++DGCFI+  F++V    + S+
Sbjct: 106 KGVDTNGLIKAVSVLEEDIRDSYSESLYSDGKKLIEMMVLDGCFILMIFLVVAGVVSHSE 165

Query: 165 DHKFPQIDPNVDLLFYQGIIPDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIPNNE 224
               P       +     I+P +  DLI LENQ+PFFLLQ +F+    E +         
Sbjct: 166 IENDP-------IFAIPWILPAIRNDLILLENQVPFFLLQTIFDRSKIEKSS-------- 225

Query: 225 FSTISFLELTFEFLRLGWVENYVQLGERIVW---SEFDPEHLVDFLRLYYISS-SDEECH 284
                  E+ F F       NY        W    + +  HL+D +R  Y+     EE  
Sbjct: 226 ----GLNEIIFHFF------NYSLQKSNTFWLKHQKVEANHLLDLIRNIYMPDVPKEEKK 285

Query: 285 DNMKINILSFFYVCRRFWNKTKKETGEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNN 344
            N  ++ +    +C                     RE ++ L +  +    E        
Sbjct: 286 SNHLLDCMMIRKICMP-------------------RESKQELDVMLKKGKQEVDISMLEE 345

Query: 345 LLSRTGSCFCRNKKKHEENDHEESYHEEKMWIPPSITELSEAGVTVKKAKKSPFITNITF 404
            + ++ S         EE D     H  KM +  S  +L   G+  K  KK+  + +I  
Sbjct: 346 GIPKSDSL--------EEEDSTTGRHHLKMVL--SARKLQLKGIKFKARKKAETLMDIRH 405

Query: 405 KNGVMEIPPIVIDDYFETITRNLIAYEHLDAKNYQEMNVISYILFMDYLISTEKDVSLLE 464
           K  +++IPP+++DD+   +  N +A+E   +   ++ ++ SY+ FM  L+ +E D   L 
Sbjct: 406 KGKLLQIPPLILDDFLIAVLLNCVAFEQYYSYCTKQ-HMTSYVAFMGCLLKSEADAMFLS 465

Query: 465 NAGIIVNDIGGSNKEVSELFNNLSKFVNRSGDISAYFEKFNGISKALCEYRDRRWNKLKA 524
             GI+ N  G S  EVS  F  + K V    D S     F G++K    Y    W+   A
Sbjct: 466 EVGILENYFG-SGDEVSRFFKVVGKDVLFDIDESYLAGIFEGVNK----YTSSGWHVQWA 500

Query: 525 SLKHNYFNTPWAGISVFAATFIIVLTLLQTIFAGISTF 556
             KH +F++PW  +S  AA  +++LT++Q  FA    F
Sbjct: 526 GFKHTHFDSPWTALSSCAALTLVILTIIQAFFAAYGYF 500

BLAST of CSPI02G23290 vs. TAIR10
Match: AT4G31980.1 (AT4G31980.1 unknown protein)

HSP 1 Score: 133.3 bits (334), Expect = 4.7e-31
Identity = 85/245 (34.69%), Postives = 136/245 (55.51%), Query Frame = 1

Query: 28  IVISIKSELEQLPVVNTKCSIYRVPKLLCQMNKTAYVPQVISIGPFHHGFKDLNATERYK 87
           +V SIK++L  L  ++TKC IY+VP  L ++N  AY P+++S GP H G ++L A E  K
Sbjct: 275 LVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQK 334

Query: 88  LQGLRNFLHRFDDRKEKSLKELVKIAQSWVEEARSCYADPIGMDDHEFVKMMLVDGCFIV 147
            + L +F+     R   SL++LV++A++W + ARSCYA+ + +   EFV+M++VDG F+V
Sbjct: 335 YRYLLSFI----PRTNSSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLV 394

Query: 148 EFFILVHNQYTTSDDHKFPQIDPNVDLLFYQG-IIPDVYFDLIKLENQLPFFLLQRLFEL 207
           E  +  H          +P++    D +F    +I DV  D+I +ENQLPFF+++ +F L
Sbjct: 395 ELLLRSH----------YPRLRGENDRIFGNSMMITDVCRDMILIENQLPFFVVKEIFLL 454

Query: 208 IPKENNPKSLIPNNEFSTISFLELTFEFLRLGWVENYVQLGERIVWSEF--DPEHLVDFL 267
                    L+   +  T S ++L           ++     RI   +F  +PEH VD L
Sbjct: 455 ---------LLNYYQQGTPSIIQLA--------QRHFSYFLSRIDDEKFITEPEHFVDLL 488

Query: 268 RLYYI 270
           R  Y+
Sbjct: 515 RSCYL 488

BLAST of CSPI02G23290 vs. TAIR10
Match: AT3G50140.1 (AT3G50140.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 120.2 bits (300), Expect = 4.1e-27
Identity = 74/185 (40.00%), Postives = 114/185 (61.62%), Query Frame = 1

Query: 373 ITELSEAGVTVKKAKKSPFITNITFKNGVMEIPPIVIDDYFETITRNLIAYE--HLDAKN 432
           +TEL EAG+  K+ K   F  +I FKNG +EIP ++I D  +++  NLIAYE  H+D+ N
Sbjct: 361 VTELREAGIKFKRRKSDRF-WDIQFKNGCLEIPKLLIHDGTKSLFSNLIAYEQCHIDSTN 420

Query: 433 YQEMNVISYILFMDYLISTEKDVSLLENAGIIVNDIGGSNKEVSELFNNLSKFVNRSGDI 492
               ++ SYI+FMD LI + +D+  L    II + + G++ EV+++FN L + V  + D+
Sbjct: 421 ----DITSYIIFMDNLIDSAEDIRYLHYYDIIEHWL-GNDSEVADVFNRLCQEV--AFDL 480

Query: 493 SAYFEKFNGISKALCEYRDRRWNKLKASLKHNYFNTPWAGISVFAATFIIVLTLLQTIFA 552
              +   + +S  +  Y +R+WN LKA+LKH YF+ PWA  S FAA  +++LTL Q+ F 
Sbjct: 481 ENTY--LSELSNKVDRYYNRKWNVLKATLKHKYFSNPWAYFSFFAAVILLLLTLFQSFFT 535

Query: 553 GISTF 556
               F
Sbjct: 541 SYPYF 535

BLAST of CSPI02G23290 vs. TAIR10
Match: AT3G50130.1 (AT3G50130.1 Plant protein of unknown function (DUF247))

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-26
Identity = 76/185 (41.08%), Postives = 108/185 (58.38%), Query Frame = 1

Query: 373 ITELSEAGVTVKKAKKSPFITNITFKNGVMEIPPIVIDDYFETITRNLIAYE--HLDAKN 432
           +TEL EAG+  +  K   F  +I FKNG +EIP ++I D  +++  NLIA+E  H+D+ N
Sbjct: 386 VTELREAGIKFRTRKTDRF-WDIRFKNGYLEIPKLLIHDGTKSLFSNLIAFEQCHIDSSN 445

Query: 433 YQEMNVISYILFMDYLISTEKDVSLLENAGIIVNDIGGSNKEVSELFNNLSKFVNRSGDI 492
               ++ SYI+FMD LI + +DV  L   GII + +G ++ EV++LFN L + V      
Sbjct: 446 ----DITSYIIFMDNLIDSSEDVRYLHYCGIIEHWLG-NDYEVADLFNRLCQEVAFDPQN 505

Query: 493 SAYFEKFNGISKALCEYRDRRWNKLKASLKHNYFNTPWAGISVFAATFIIVLTLLQTIFA 552
           S   +  N + +       R+WN LKA LKH YFN PWA  S FAA  ++VLTL Q+ F 
Sbjct: 506 SYLSQLSNKVDRNY----SRKWNVLKAILKHKYFNNPWAYFSFFAALVLLVLTLFQSFFT 560

Query: 553 GISTF 556
               F
Sbjct: 566 AYPYF 560

BLAST of CSPI02G23290 vs. NCBI nr
Match: gi|659088834|ref|XP_008445186.1| (PREDICTED: putative UPF0481 protein At3g02645 [Cucumis melo])

HSP 1 Score: 1032.3 bits (2668), Expect = 3.0e-298
Identity = 518/556 (93.17%), Postives = 533/556 (95.86%), Query Frame = 1

Query: 1   MENSENNDDQVSNEAVISEVKRKHCGDIVISIKSELEQLPVVNTKCSIYRVPKLLCQMNK 60
           MENSE N+DQVSNEAVISEVKRKHCGDIVISIKS+LEQLP VNT+CSIYRVPKLLCQMN+
Sbjct: 1   MENSEINNDQVSNEAVISEVKRKHCGDIVISIKSKLEQLPAVNTECSIYRVPKLLCQMNR 60

Query: 61  TAYVPQVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRK-EKSLKELVKIAQSWVEE 120
            AYVPQVISIGPFHHG KDLNATERYKLQGLRNFL R DD K EKSL+ELVKIAQSWVEE
Sbjct: 61  IAYVPQVISIGPFHHGSKDLNATERYKLQGLRNFLRRLDDHKAEKSLEELVKIAQSWVEE 120

Query: 121 ARSCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSDDHKFPQIDPNVDLLFYQG 180
           AR CYADPI MDDHEFVKMMLVDGCFIVEFFILVHNQYTTS DHKFPQIDPNVDLLFYQG
Sbjct: 121 ARRCYADPIDMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSADHKFPQIDPNVDLLFYQG 180

Query: 181 IIPDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIPNNEFSTISFLELTFEFLRLGW 240
           IIPDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIP+NEFSTISFLELTFEFLRLGW
Sbjct: 181 IIPDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIPDNEFSTISFLELTFEFLRLGW 240

Query: 241 VENYVQLGERIVWSEFDPEHLVDFLRLYYISSSDEECHDNMKINILSFFYVCRRFWNKTK 300
           VENYVQLGERIVWSEFDPEHLVDFLRLYYISSSD+E  DN KINILSFFY+CRR+ N+T 
Sbjct: 241 VENYVQLGERIVWSEFDPEHLVDFLRLYYISSSDDEYRDNKKINILSFFYLCRRYLNRTN 300

Query: 301 KETGEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFCRNKKKHEENDHE 360
           KET  KKKVHAEN EKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCF RNKKKH ENDH+
Sbjct: 301 KETSGKKKVHAENGEKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFGRNKKKHVENDHD 360

Query: 361 ESYHEEKMWIPPSITELSEAGVTVKKAKKSPFITNITFKNGVMEIPPIVIDDYFETITRN 420
           ESYHEEKMWIPPSITELSEAGVT+KKAKKSPFITNITFKNGV+EIPPIVIDDYFETITRN
Sbjct: 361 ESYHEEKMWIPPSITELSEAGVTIKKAKKSPFITNITFKNGVLEIPPIVIDDYFETITRN 420

Query: 421 LIAYEHLDAKNYQEMNVISYILFMDYLISTEKDVSLLENAGIIVNDIGGSNKEVSELFNN 480
           LIAYEHLDAKNYQEMNVISYILFMDYLIS EKDVSLLE AGIIVNDIGGS+KEVSELFNN
Sbjct: 421 LIAYEHLDAKNYQEMNVISYILFMDYLISIEKDVSLLEKAGIIVNDIGGSDKEVSELFNN 480

Query: 481 LSKFVNRSGDISAYFEKFNGISKALCEYRDRRWNKLKASLKHNYFNTPWAGISVFAATFI 540
           LSKFVNRSGDISAYFEKFNGISKALCE+RDRRWNKLKASLKHNYFNTPWAGISVFAATFI
Sbjct: 481 LSKFVNRSGDISAYFEKFNGISKALCEHRDRRWNKLKASLKHNYFNTPWAGISVFAATFI 540

Query: 541 IVLTLLQTIFAGISTF 556
           IVLTLLQTIFAGISTF
Sbjct: 541 IVLTLLQTIFAGISTF 556

BLAST of CSPI02G23290 vs. NCBI nr
Match: gi|778672484|ref|XP_011649818.1| (PREDICTED: UPF0481 protein At3g47200-like [Cucumis sativus])

HSP 1 Score: 715.3 bits (1845), Expect = 8.2e-203
Identity = 406/568 (71.48%), Postives = 441/568 (77.64%), Query Frame = 1

Query: 1   MENSENNDDQVSNEAVISEVKRKHCGDIVISIKSELEQLPVVNTKCSIYRVPKLLCQMNK 60
           ME  ENND                C DIVISIKS+L+QL VVNT+C+IYRVPKLLCQMN 
Sbjct: 1   MEVGENND----------------CRDIVISIKSKLKQLHVVNTECNIYRVPKLLCQMNM 60

Query: 61  TAYVPQVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRK-EKSLKELVKIAQSWVEE 120
           TAYVPQVISIGP HHG KDLNATE YKLQGLRNFL RFDD   EK L+ LV+I Q +V  
Sbjct: 61  TAYVPQVISIGPLHHGSKDLNATECYKLQGLRNFLRRFDDSYGEKLLENLVEITQFYVGR 120

Query: 121 ARSCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSDDHKFPQIDPNVDLLFY-Q 180
           ARSCYADPI MDDHEFVKMMLVDGCFIVE FILVHN  TTSDDHKF +ID NVDLLFY Q
Sbjct: 121 ARSCYADPIDMDDHEFVKMMLVDGCFIVELFILVHN-ITTSDDHKFSKIDQNVDLLFYYQ 180

Query: 181 GIIPDVYFDLIKLENQLPFFLLQRLFELIPKENNP-KSLIPNNEFST-------ISFLEL 240
           GIIPDVYFDLIKLENQLPFF+LQ LF+LIP++NN  KSLI N +          I FLEL
Sbjct: 181 GIIPDVYFDLIKLENQLPFFILQLLFDLIPRDNNQSKSLILNRKSPILDKNSLFIPFLEL 240

Query: 241 TFEFLRLGWVENYVQLGER-IVWSEFDPEHLVDFLRLYY--ISSSDEECHDNMKINILSF 300
           TFE LRLGWV++YVQLG+R I   EF P+HL+DFL LYY   SS+D+ECH+         
Sbjct: 241 TFENLRLGWVKDYVQLGKRSITLHEFYPKHLLDFLTLYYENSSSNDDECHN--------- 300

Query: 301 FYVCRRFWNKTKKETGEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFC 360
                        ET EKKKVHAENR+ Q+TLSIFK+TY      K KNNL S    CF 
Sbjct: 301 -------------ETSEKKKVHAENRDNQKTLSIFKQTY-----LKVKNNLCS----CFR 360

Query: 361 RNKKKHEENDHEESYHEEKMWIPPSITELSEAGVTVKKAKKSPFITNITFKNGVMEIPPI 420
           RNKK H ENDHE+     KM+IPPSITELSEAGVT KK KK P ITNITFKNGV+EIPPI
Sbjct: 361 RNKK-HGENDHEK-----KMYIPPSITELSEAGVTFKKPKKLPIITNITFKNGVLEIPPI 420

Query: 421 VIDDYFETITRNLIAYEHLDAKNYQEMNVISYILFMDYLISTEKDVSLLENAGIIVNDIG 480
           VID YFETITRNLIAYEH+D K Y+EM VISYILFMDYLISTEKDVSLLE AGIIVNDIG
Sbjct: 421 VIDHYFETITRNLIAYEHMD-KIYKEMKVISYILFMDYLISTEKDVSLLEKAGIIVNDIG 480

Query: 481 GSNKEVSELFNNLSKFVNRSGDISAYFEKFNGISKALCEYRDRRWNKLKASLKHNYFNTP 540
           GS+KEVS+LFNNLSKFVNRS + S YF+KF GISKALCE+RDRRWNKLKASLKHNYFNTP
Sbjct: 481 GSHKEVSKLFNNLSKFVNRSDNNSEYFKKFQGISKALCEHRDRRWNKLKASLKHNYFNTP 513

Query: 541 WAGISVFAATFIIVLTLLQTIFAGISTF 556
           WA ISVFAAT +IVLTLLQTIFA ISTF
Sbjct: 541 WASISVFAATLVIVLTLLQTIFAVISTF 513

BLAST of CSPI02G23290 vs. NCBI nr
Match: gi|700207808|gb|KGN62927.1| (hypothetical protein Csa_2G380070 [Cucumis sativus])

HSP 1 Score: 430.3 bits (1105), Expect = 5.3e-117
Identity = 230/333 (69.07%), Postives = 246/333 (73.87%), Query Frame = 1

Query: 63  YVPQVISIGPFHHGFKDLNATERYKLQGLRNFLHRFDDRKEKSLKELVKIAQ-SWVEEAR 122
           YVPQVISIGPFHHG KDLN TERYK QGLRNFL R +D KEKSL+EL KIAQ SWVEEA 
Sbjct: 19  YVPQVISIGPFHHGSKDLNVTERYKFQGLRNFLRRLNDHKEKSLEELTKIAQLSWVEEAC 78

Query: 123 SCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSDDHKFPQIDPNVDLLFYQGII 182
            CY  PI MD                              DH+F      V ++   GII
Sbjct: 79  GCYIYPIDMD------------------------------DHEF------VKMMCVDGII 138

Query: 183 PDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIPNNEFSTISFLELTFEFLRLGWVE 242
           PDVYFDLIKLENQLPFFLLQRLFELIPKENN KSLIPNNEFSTISFLELTF+FL LG  E
Sbjct: 139 PDVYFDLIKLENQLPFFLLQRLFELIPKENNLKSLIPNNEFSTISFLELTFQFLHLGCAE 198

Query: 243 NYVQLGERIVWSEFDPEHLVDFLRLYYISSSDEECHDNMKINILSFFYVCRRFWNKTKKE 302
           NYVQLGERIVWS+FDP+HLVDFL+LYYISSS +EC ++ KI ILSF Y C R+ N T KE
Sbjct: 199 NYVQLGERIVWSKFDPKHLVDFLKLYYISSSADECREDKKIRILSFSYSCCRYLNMTNKE 258

Query: 303 TGEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGSCFCRNKKKHEENDHEES 362
           T EKKKVHAEN EKQRT SIFKRTYSP+YWTK KNNLLSR G CF RNK KHEEN     
Sbjct: 259 TSEKKKVHAENGEKQRTHSIFKRTYSPKYWTKGKNNLLSRAGLCFGRNKTKHEEN----- 305

Query: 363 YHEEKMWIPPSITELSEAGVTVKKAKKSPFITN 395
                MWIPPSITE+S+ GV VKKAKKSPFITN
Sbjct: 319 -----MWIPPSITEISKVGVIVKKAKKSPFITN 305

BLAST of CSPI02G23290 vs. NCBI nr
Match: gi|659089573|ref|XP_008445583.1| (PREDICTED: LOW QUALITY PROTEIN: UPF0481 protein At3g47200-like [Cucumis melo])

HSP 1 Score: 407.1 bits (1045), Expect = 4.8e-110
Identity = 268/575 (46.61%), Postives = 339/575 (58.96%), Query Frame = 1

Query: 9   DQVSNEAV--ISEVKRKHCGDIVISIKSELEQLPVVNTKCSIYRVPKLLCQMNKTAYVPQ 68
           + VSN  +  I E ++  C ++VISI+  L Q+  +N  CSIYR+PK L +MN  AY PQ
Sbjct: 61  ESVSNNEMPQIIEAQQDLCDNVVISIQKMLNQMHSINGDCSIYRIPKQLREMNPKAYTPQ 120

Query: 69  VISIGPFHHGFK--DLNATERYKLQGLRNFLHRF-----------DDRKEKSLKELVKIA 128
           +ISIGPFHH  +  D  ATE+YKLQ L NFL R            D +K +SL +L+KI 
Sbjct: 121 LISIGPFHHYRRQNDFKATEQYKLQALVNFLRRINNNNETMEEDQDVKKSRSLDDLLKIG 180

Query: 129 ---------QSWVEEARSCYADPIG-MDDHEFVKMMLVDGCFIVEFFILVHNQYTTS--D 188
                      W+ E R+CY++PI  MDDH FV MML+D CFIVE FI    QY  S   
Sbjct: 181 TLKVLVEKVHCWMTETRNCYSEPIDYMDDHNFVIMMLLDACFIVESFI---QQYDASYPH 240

Query: 189 DHKFPQIDPNVD-LLFYQGIIPDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIPNN 248
           D KFP+I  NVD LL Y     D+  DLIKLENQ+PFFLLQ +F +IP+ + P       
Sbjct: 241 DRKFPRIQDNVDFLLLYPETSQDISNDLIKLENQVPFFLLQHMFHMIPQHSPP------- 300

Query: 249 EFSTISFLELTFEFLRLGWVENYVQLGERIVWSEFDPEHLVDFLRLYYISSSDEECHDNM 308
               +SF+ LT+  L   ++  Y      IV +E  P+HLV FL  Y+I S      D+M
Sbjct: 301 ----VSFINLTYSALYDWFISTYKICSVGIVPNE--PKHLVHFLSFYFIPSHTT---DDM 360

Query: 309 KINILSFFYVCRRFWNKTKKETGEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLS 368
                          NK + +  E KK +  N      LS F   +  + W    N    
Sbjct: 361 -------------IQNKQEIKNSENKKKNNYN-----FLSFFSTLFCCQLWQNPDN---- 420

Query: 369 RTGSCFCRNKKKHEENDHEESYHEEKMWIPPSITELSEAGVTVKKAKKSPFITNITFKNG 428
                    KKK   N           + PPSITEL E+GVT++KAK + ++TNITFKNG
Sbjct: 421 ---------KKKMIRN-----------FCPPSITELCESGVTIEKAKNAKYLTNITFKNG 480

Query: 429 VMEIPPIVIDDYFETITRNLIAYEHLDAKNYQEMNVISYILFMDYLISTEKDVSLLENAG 488
           V++IP + I D FE + RNLIA+E   A N + M    YILFMD LISTEKDV LL N+G
Sbjct: 481 VLKIPHLHIYDEFELMFRNLIAFEQFPAGN-ENMYATQYILFMDDLISTEKDVRLLVNSG 540

Query: 489 IIVNDIGGSNKEVSELFNNLSKFVNRSGDISAYFEKFNGISKALCEYRDRRWNKLKASLK 548
           +I+N+IGGS+KEVSELFNNL KFVN+    S Y   FN ISKAL ++ + RWNK KASLK
Sbjct: 541 VIINNIGGSDKEVSELFNNLGKFVNQCP--SPYM--FNHISKALRKHCNGRWNKAKASLK 569

Query: 549 HNYFNTPWAGISVFAATFIIVLTLLQTIFAGISTF 556
           HNYFNTPWA IS FAA+F+++LTLLQTIF+GIS F
Sbjct: 601 HNYFNTPWAFISFFAASFLVLLTLLQTIFSGISAF 569

BLAST of CSPI02G23290 vs. NCBI nr
Match: gi|659088828|ref|XP_008445182.1| (PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo])

HSP 1 Score: 381.7 bits (979), Expect = 2.2e-102
Identity = 255/572 (44.58%), Postives = 334/572 (58.39%), Query Frame = 1

Query: 1   MENSENNDDQVSNEA-------VISEVKRKHCGDIVISIKSELEQLPVVNTKCSI-YRVP 60
           MENSE  + +V N+         ISE++ K C ++VISI   L  LP +N KC I Y+V 
Sbjct: 1   MENSEIIETKVENDICDDELGETISEIE-KVCDNVVISIDKILGGLPRINPKCHIIYQVS 60

Query: 61  KLLCQMNKTAYVPQVISIGPFHHGFK-DLNATERYKLQGLRNFLHRFDDRKE-KSLKELV 120
           K L +MN  AY PQ ISIGPFHH  + DL A E YKLQG  NFLHR ++ ++ +S KE V
Sbjct: 61  KELREMNDKAYAPQFISIGPFHHRTRNDLIANEHYKLQGFNNFLHRINNYEQIESSKEFV 120

Query: 121 KIAQSWVEEARSCYADPIGMDDHEFVKMMLVDGCFIVEFFILVHNQYTTSD-----DHKF 180
           K    WV+EA +CYA+PI M++ EFV MMLVD CFI+EFFIL+ + +   D     D  F
Sbjct: 121 KKCHGWVKEAWNCYAEPINMNEEEFVLMMLVDACFILEFFILLIDDHYGGDYLFEADQIF 180

Query: 181 PQIDPNVDLLFYQGIIPDVYFDLIKLENQLPFFLLQRLFELIPKENNPKSLIPNNEFSTI 240
            QI   VD  FY+G+  ++  DLIKLENQ+PFFLLQ LF+L+PK + P  + P       
Sbjct: 181 -QIQDMVDFSFYRGVFFEILIDLIKLENQVPFFLLQNLFDLMPKHDVP--MFP------- 240

Query: 241 SFLELTFEFLR-LGWVENYVQLGERIVWSEFDPEHLVDFLRLYYISSSDEECHDNMKINI 300
           S +++T E L   G+V  Y ++ +        P+HL+DFL  Y+      + H   K N 
Sbjct: 241 SLIDITSEILTWFGFVGKY-KINDLY---HKKPKHLLDFLSFYFFPLLPNDDHIRFKQN- 300

Query: 301 LSFFYVCRRFWNKTKKETGEKKKVHAENREKQRTLSIFKRTYSPEYWTKAKNNLLSRTGS 360
                              E+K     N    R    F R   P +W K  N+       
Sbjct: 301 -------------------ERKNSDQNNNNLLR----FFRPLFPAHWLKKNNDSFGVPSL 360

Query: 361 CFCRNKKKHEENDHEESYHEEKMWIPPSITELSEAGVTVKKAKKSPF-ITNITFKNGVME 420
           C   NK+        E+Y       PPSITEL EAGVT+K AK+      NI FKNGV+E
Sbjct: 361 CCFSNKEAETREKDSENYFRLS---PPSITELCEAGVTIKAAKREDLCFMNIGFKNGVLE 420

Query: 421 IPPIVIDDYFETITRNLIAYEHLDAKNYQEMNVISYILFMDYLISTEKDVSLLENAGIIV 480
           IP I ID  FE + RN+IA++   A N ++M  I Y+LF+D LI+TE+D  LL  AGII+
Sbjct: 421 IPCIDIDCTFEVVIRNVIAFDQYPAGN-EKMYAIHYVLFLDDLINTEQDAHLLTKAGIII 480

Query: 481 NDIGGSNKEVSELFNNLSKFVNRSGDISAYFEKFNGISKALCEYRDRRWNKLKASLKHNY 540
           N  GGS+K+++E+FN  SKFV  +  I ++F+  N   KAL  + + RWN  KASLKHNY
Sbjct: 481 NTFGGSDKDITEMFNRFSKFV--TFPICSHFDDIN---KALRMHCNGRWNMAKASLKHNY 524

Query: 541 FNTPWAGISVFAATFIIVLTLLQTIFAGISTF 556
           FNTPWA IS  AATF+I+LT+LQTIF+ ISTF
Sbjct: 541 FNTPWAIISFGAATFLIILTILQTIFSAISTF 524

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Y3720_ARATH2.5e-1834.16UPF0481 protein At3g47200 OS=Arabidopsis thaliana GN=At3g47200 PE=2 SV=1[more]
Y3264_ARATH1.4e-1328.00Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana GN=At3g02645 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LQ64_CUCSA5.7e-20371.48Uncharacterized protein OS=Cucumis sativus GN=Csa_2G381630 PE=4 SV=1[more]
A0A0A0LQ49_CUCSA3.7e-11769.07Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380070 PE=4 SV=1[more]
A0A0A0K4A0_CUCSA4.5e-9983.03Uncharacterized protein OS=Cucumis sativus GN=Csa_7G068570 PE=4 SV=1[more]
A0A0A0K2S5_CUCSA5.7e-8673.31Uncharacterized protein OS=Cucumis sativus GN=Csa_7G068560 PE=4 SV=1[more]
A0A0A0LPJ5_CUCSA1.7e-6942.17Uncharacterized protein OS=Cucumis sativus GN=Csa_2G380630 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G50170.15.7e-5330.52 Plant protein of unknown function (DUF247)[more]
AT3G44710.12.4e-4327.41 Plant protein of unknown function (DUF247)[more]
AT4G31980.14.7e-3134.69 unknown protein[more]
AT3G50140.14.1e-2740.00 Plant protein of unknown function (DUF247)[more]
AT3G50130.11.6e-2641.08 Plant protein of unknown function (DUF247)[more]
Match NameE-valueIdentityDescription
gi|659088834|ref|XP_008445186.1|3.0e-29893.17PREDICTED: putative UPF0481 protein At3g02645 [Cucumis melo][more]
gi|778672484|ref|XP_011649818.1|8.2e-20371.48PREDICTED: UPF0481 protein At3g47200-like [Cucumis sativus][more]
gi|700207808|gb|KGN62927.1|5.3e-11769.07hypothetical protein Csa_2G380070 [Cucumis sativus][more]
gi|659089573|ref|XP_008445583.1|4.8e-11046.61PREDICTED: LOW QUALITY PROTEIN: UPF0481 protein At3g47200-like [Cucumis melo][more]
gi|659088828|ref|XP_008445182.1|2.2e-10244.58PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004158DUF247_pln
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G23290.1CSPI02G23290.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 48..542
score: 2.0
NoneNo IPR availablePANTHERPTHR31549FAMILY NOT NAMEDcoord: 1..273
score: 5.1E-137coord: 357..553
score: 5.1E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G23290Cucurbita maxima (Rimu)cmacpiB165
CSPI02G23290Cucurbita maxima (Rimu)cmacpiB442
CSPI02G23290Cucurbita moschata (Rifu)cmocpiB150
CSPI02G23290Cucurbita moschata (Rifu)cmocpiB431
CSPI02G23290Cucurbita moschata (Rifu)cmocpiB788
CSPI02G23290Cucumber (Chinese Long) v2cpicuB067
CSPI02G23290Cucumber (Chinese Long) v2cpicuB101
CSPI02G23290Melon (DHL92) v3.5.1cpimeB118
CSPI02G23290Melon (DHL92) v3.5.1cpimeB150
CSPI02G23290Watermelon (Charleston Gray)cpiwcgB107
CSPI02G23290Watermelon (Charleston Gray)cpiwcgB100
CSPI02G23290Watermelon (Charleston Gray)cpiwcgB166
CSPI02G23290Watermelon (Charleston Gray)cpiwcgB168
CSPI02G23290Watermelon (97103) v1cpiwmB109
CSPI02G23290Watermelon (97103) v1cpiwmB124
CSPI02G23290Cucurbita pepo (Zucchini)cpecpiB101
CSPI02G23290Cucurbita pepo (Zucchini)cpecpiB544
CSPI02G23290Cucurbita pepo (Zucchini)cpecpiB811
CSPI02G23290Bottle gourd (USVL1VR-Ls)cpilsiB112
CSPI02G23290Bottle gourd (USVL1VR-Ls)cpilsiB136
CSPI02G23290Melon (DHL92) v3.6.1cpimedB102
CSPI02G23290Melon (DHL92) v3.6.1cpimedB114
CSPI02G23290Cucumber (Gy14) v2cgybcpiB061
CSPI02G23290Cucumber (Gy14) v2cgybcpiB312
CSPI02G23290Silver-seed gourdcarcpiB0155
CSPI02G23290Silver-seed gourdcarcpiB0225
CSPI02G23290Silver-seed gourdcarcpiB1010
CSPI02G23290Cucumber (Chinese Long) v3cpicucB081
CSPI02G23290Cucumber (Chinese Long) v3cpicucB084
CSPI02G23290Cucumber (Chinese Long) v3cpicucB102
CSPI02G23290Cucumber (Chinese Long) v3cpicucB125
CSPI02G23290Watermelon (97103) v2cpiwmbB110
CSPI02G23290Watermelon (97103) v2cpiwmbB160
CSPI02G23290Watermelon (97103) v2cpiwmbB162
CSPI02G23290Watermelon (97103) v2cpiwmbB172
CSPI02G23290Wax gourdcpiwgoB160
CSPI02G23290Wax gourdcpiwgoB208
CSPI02G23290Wild cucumber (PI 183967)cpicpiB055
CSPI02G23290Wild cucumber (PI 183967)cpicpiB072
CSPI02G23290Wild cucumber (PI 183967)cpicpiB097
CSPI02G23290Cucumber (Gy14) v1cgycpiB101
CSPI02G23290Cucumber (Gy14) v1cgycpiB239