CSPI02G01300 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G01300
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionHeavy metal transport/detoxification superfamily protein
LocationChr2 : 886459 .. 887403 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATTGCAATGGCTGTGCTAGAAGAGTTGAGAAACATATATCCAAGATACAAGGTAATGTTTTTTTTTTAAATAATAATAATTATTATTACGAGGTAAATTGTAAAAAAAATGACAAAATGTAAATAGTTTGTATATTTTTAAATTTATTGAAAAATACAAAACTAAAAGTATAGTTGATGTCAATTCTATGAATAATAATTAAGTATATAACAACATTTCACAAATGTTGTAAATATAACAAAATTTATCGATGATATATTATGATAGATCAATATTTACAACATGGTATATTAGTGATAGATTTTGACAAATTTGACTATATTTGCAATTTTTTAAAAATATTTTATATATTTAATTATTTTGAATCTAATGACTATATTTTATAATATATATATGTCCAAATTGGTGGTTTTGAAATTTGGTTCAAATCCCCGCATATATTGTTTCTACATCCCAATTTCGTTAATAAAACAGTCCATGAACTAAAAGATTTGTACAGTACCCATCAAACATGCTTAATCATCTAATAATGTTTTACATAAATAACTATGTATACTTAATATCATATTTATTAATAAAGTAGGGAGATTGTATTAGATGACAAAAACAAAAATAAAAAAACAAATTTATAGTATTTTTGTTTATATTACAAATTTGATAAATTTGACATATTTTTAAATTTGCCATTTAAAAAATGTAGATGACGTGGAATCTGTTATTATATTTTCTTTTGTTATTTTTGTAAACTCGCCAGTAAAGTAGGTATCAAAATAAAATTTAAATCTATGCTATCTATCTTATAATACTTGCTTTTAGGCGTGGAATCATGGAAGGTAGATATGGAAAGGGAGACTGTGGTTGTTACCGGAGATGTTTTTCCTTTTGAAGTCATGCAATGTATTTCTAAAGTGAAGAGTGTAGAGATTTTGGAGCCTCAAGTT

mRNA sequence

ATGCATTGCAATGGCTGTGCTAGAAGAGTTGAGAAACATATATCCAAGATACAAGGCGTGGAATCATGGAAGGTAGATATGGAAAGGGAGACTGTGGTTGTTACCGGAGATGTTTTTCCTTTTGAAGTCATGCAATGTATTTCTAAAGTGAAGAGTGTAGAGATTTTGGAGCCTCAAGTT

Coding sequence (CDS)

ATGCATTGCAATGGCTGTGCTAGAAGAGTTGAGAAACATATATCCAAGATACAAGGCGTGGAATCATGGAAGGTAGATATGGAAAGGGAGACTGTGGTTGTTACCGGAGATGTTTTTCCTTTTGAAGTCATGCAATGTATTTCTAAAGTGAAGAGTGTAGAGATTTTGGAGCCTCAAGTT
Homology
[Close]
BLAST of CSPI02G01300 vs. TrEMBL
Match: A0A0A0LFW6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G005300 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.7e-26
Identity = 60/60 (100.00%), Postives = 60/60 (100.00%), Query Frame = 1 

Query: 1  MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV 60
          MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV
Sbjct: 1  MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV 60

[Close]
BLAST of CSPI02G01300 vs. TrEMBL
Match: A0A022QIY3_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a015876mg PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 7.0e-14
Identity = 34/59 (57.63%), Postives = 51/59 (86.44%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQ 60
           MHCNGCAR++EKHISK++GV S+KVD+E + VVVTGD+ PF+V++ +SK+K+ ++ + Q
Sbjct: 83  MHCNGCARKIEKHISKLEGVISYKVDLETKMVVVTGDIAPFQVLESVSKIKNAQLWDDQ 141

[Close]
BLAST of CSPI02G01300 vs. TrEMBL
Match: M0TTR4_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 9.1e-14
Identity = 35/55 (63.64%), Postives = 48/55 (87.27%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHCNGCAR++EKHISK++GV S+KVD+E++ VVV GD+ PFEV++ +SKVK  E+
Sbjct: 100 MHCNGCARKIEKHISKMEGVSSFKVDLEKKKVVVVGDITPFEVLESVSKVKFAEL 154

[Close]
BLAST of CSPI02G01300 vs. TrEMBL
Match: M5VRG3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013199mg PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.2e-13
Identity = 38/55 (69.09%), Postives = 47/55 (85.45%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHCNGCAR+VEKHISKI+GV S+KVD+E + VVV GDV P EV++ +SKVK+ EI
Sbjct: 76  MHCNGCARKVEKHISKIEGVTSYKVDLESKMVVVIGDVLPREVLESVSKVKNTEI 130

[Close]
BLAST of CSPI02G01300 vs. TrEMBL
Match: G7KNF6_MEDTR (Heavy metal transport/detoxification superfamily protein OS=Medicago truncatula GN=MTR_6g086020 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.6e-13
Identity = 35/55 (63.64%), Postives = 48/55 (87.27%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHC+GCAR+VEKHISK++GV S+KVD++ + VVV GD+ PFEV++ +SKVK+ EI
Sbjct: 73  MHCHGCARKVEKHISKLEGVSSYKVDLDTKMVVVMGDILPFEVLESVSKVKNAEI 127

[Close]
BLAST of CSPI02G01300 vs. TAIR10
Match: AT3G24450.1 (AT3G24450.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 4.5e-12
Identity = 28/55 (50.91%), Postives = 43/55 (78.18%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHC GCA++VEKHISK+ GV  +KV++E + VVV G++ P +V++ I KVK+ ++
Sbjct: 82  MHCYGCAKKVEKHISKLDGVTWYKVELESKKVVVKGNILPVDVLESICKVKNAQL 136

[Close]
BLAST of CSPI02G01300 vs. TAIR10
Match: AT5G02600.2 (AT5G02600.2 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 5.0e-11
Identity = 27/54 (50.00%), Postives = 39/54 (72.22%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVE 55
           +HC GCA +V+KH+SK++GV S+ +D   + V VTGDV P  V+  ISKVK+ +
Sbjct: 258 LHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLTVLASISKVKNAQ 311

[Close]
BLAST of CSPI02G01300 vs. TAIR10
Match: AT3G53530.2 (AT3G53530.2 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 62.8 bits (151), Expect = 8.5e-11
Identity = 28/58 (48.28%), Postives = 40/58 (68.97%), Query Frame = 1 

Query: 2   HCNGCARRVEKHISKIQ-GVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEP 59
           HC GC  +V+KH+SK+Q GV S+ +D   + V VTGD+ P EV+ C+SKVK+ +   P
Sbjct: 179 HCRGCQGKVKKHLSKMQVGVTSFNIDFASKKVTVTGDITPLEVLGCLSKVKNAQFWTP 236

[Close]
BLAST of CSPI02G01300 vs. TAIR10
Match: AT2G37390.1 (AT2G37390.1 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 61.6 bits (148), Expect = 1.9e-10
Identity = 24/54 (44.44%), Postives = 38/54 (70.37%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVE 55
           +HC GC  +V KH++++QGV S+ +D   + V VTGD+ P E++  ISKVK+ +
Sbjct: 189 LHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKNAQ 242

[Close]
BLAST of CSPI02G01300 vs. TAIR10
Match: AT2G28660.1 (AT2G28660.1 Chloroplast-targeted copper chaperone protein)

HSP 1 Score: 55.5 bits (132), Expect = 1.3e-08
Identity = 23/55 (41.82%), Postives = 37/55 (67.27%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           +HC GC  +V KHISK++GV S+ +D+  + V V G + P  +++ ISKVK  ++
Sbjct: 191 IHCKGCEGKVRKHISKMEGVTSYTIDLATKKVTVVGKITPVGLVESISKVKFAQL 245

[Close]
BLAST of CSPI02G01300 vs. NCBI nr
Match: gi|700205514|gb|KGN60633.1| (hypothetical protein Csa_2G005300 [Cucumis sativus])

HSP 1 Score: 125.2 bits (313), Expect = 3.9e-26
Identity = 60/60 (100.00%), Postives = 60/60 (100.00%), Query Frame = 1 

Query: 1  MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV 60
          MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV
Sbjct: 1  MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQV 60

[Close]
BLAST of CSPI02G01300 vs. NCBI nr
Match: gi|659071114|ref|XP_008458177.1| (PREDICTED: uncharacterized protein LOC103497691 [Cucumis melo])

HSP 1 Score: 113.2 bits (282), Expect = 1.5e-22
Identity = 55/59 (93.22%), Postives = 56/59 (94.92%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILEPQ 60
           MHCNGCARRVEK ISKIQGVESWKVDMERE VVVTGDVFPFEVM+CISKVKSVEILE Q
Sbjct: 87  MHCNGCARRVEKLISKIQGVESWKVDMERERVVVTGDVFPFEVMECISKVKSVEILESQ 145

[Close]
BLAST of CSPI02G01300 vs. NCBI nr
Match: gi|719980545|ref|XP_010249835.1| (PREDICTED: copper transport protein ATX1 [Nelumbo nucifera])

HSP 1 Score: 85.9 bits (211), Expect = 2.6e-14
Identity = 38/57 (66.67%), Postives = 49/57 (85.96%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEILE 58
           MHCNGCAR+VEKHISK++GV S+KVD+E + VVV GDV PFEV++ +SKVK+ E+ E
Sbjct: 79  MHCNGCARKVEKHISKMEGVTSYKVDLESKKVVVIGDVLPFEVLESVSKVKNAELWE 135

[Close]
BLAST of CSPI02G01300 vs. NCBI nr
Match: gi|658004639|ref|XP_008337448.1| (PREDICTED: uncharacterized protein LOC103400574 [Malus domestica])

HSP 1 Score: 85.9 bits (211), Expect = 2.6e-14
Identity = 39/55 (70.91%), Postives = 48/55 (87.27%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHCNGCA++VEKHISKI+GV S+KVD+E + VVV GDV PFEV++ ISKVK+ EI
Sbjct: 76  MHCNGCAKKVEKHISKIEGVTSYKVDLESKMVVVIGDVLPFEVLESISKVKNAEI 130

[Close]
BLAST of CSPI02G01300 vs. NCBI nr
Match: gi|470101233|ref|XP_004287081.1| (PREDICTED: uncharacterized protein LOC101298054 [Fragaria vesca subsp. vesca])

HSP 1 Score: 85.1 bits (209), Expect = 4.5e-14
Identity = 38/55 (69.09%), Postives = 48/55 (87.27%), Query Frame = 1 

Query: 1   MHCNGCARRVEKHISKIQGVESWKVDMERETVVVTGDVFPFEVMQCISKVKSVEI 56
           MHCNGCAR+VEKHISK++GV S+KVD+E + VVV GDV P+EV+Q +SKVK+ EI
Sbjct: 76  MHCNGCARKVEKHISKMEGVTSYKVDLESKMVVVIGDVLPYEVVQSVSKVKNAEI 130

The following BLAST results are available for this feature:
BLAST of CSPI02G01300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of CSPI02G01300 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LFW6_CUCSA2.7e-26100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G005300 PE=4 SV=1[more]
A0A022QIY3_ERYGU7.0e-1457.63Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a015876mg PE=4 SV=1[more]
M0TTR4_MUSAM9.1e-1463.64Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1[more]
M5VRG3_PRUPE1.2e-1369.09Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa013199mg PE=4 SV=1[more]
G7KNF6_MEDTR1.6e-1363.64Heavy metal transport/detoxification superfamily protein OS=Medicago truncatula ... [more]
BLAST of CSPI02G01300 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G24450.14.5e-1250.91 Heavy metal transport/detoxification superfamily protein [more]
AT5G02600.25.0e-1150.00 Heavy metal transport/detoxification superfamily protein [more]
AT3G53530.28.5e-1148.28 Chloroplast-targeted copper chaperone protein[more]
AT2G37390.11.9e-1044.44 Chloroplast-targeted copper chaperone protein[more]
AT2G28660.11.3e-0841.82 Chloroplast-targeted copper chaperone protein[more]
BLAST of CSPI02G01300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700205514|gb|KGN60633.1|3.9e-26100.00hypothetical protein Csa_2G005300 [Cucumis sativus][more]
gi|659071114|ref|XP_008458177.1|1.5e-2293.22PREDICTED: uncharacterized protein LOC103497691 [Cucumis melo][more]
gi|719980545|ref|XP_010249835.1|2.6e-1466.67PREDICTED: copper transport protein ATX1 [Nelumbo nucifera][more]
gi|658004639|ref|XP_008337448.1|2.6e-1470.91PREDICTED: uncharacterized protein LOC103400574 [Malus domestica][more]
gi|470101233|ref|XP_004287081.1|4.5e-1469.09PREDICTED: uncharacterized protein LOC101298054 [Fragaria vesca subsp. vesca][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006121HMA_dom
Vocabulary: Biological Process
TermDefinition
GO:0030001metal ion transport
Vocabulary: Molecular Function
TermDefinition
GO:0046872metal ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030001 metal ion transport
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G01300.1CSPI02G01300.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 1..40
score: 4.1
IPR006121Heavy metal-associated domain, HMAPROFILEPS50846HMA_2coord: 1..60
score: 12
IPR006121Heavy metal-associated domain, HMAunknownSSF55008HMA, heavy metal-associated domaincoord: 1..50
score: 1.1
NoneNo IPR availableGENE3DG3DSA:3.30.70.100coord: 1..50
score: 2.5
NoneNo IPR availablePANTHERPTHR22814COPPER TRANSPORT PROTEIN ATOX1-RELATEDcoord: 1..51
score: 4.6
NoneNo IPR availablePANTHERPTHR22814:SF104SUBFAMILY NOT NAMEDcoord: 1..51
score: 4.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G01300Csa2G005300Cucumber (Chinese Long) v2cpicuB065
CSPI02G01300MELO3C025323Melon (DHL92) v3.5.1cpimeB110
CSPI02G01300CsaV3_1G030750Cucumber (Chinese Long) v3cpicucB073
CSPI02G01300Cla97C06G119580Watermelon (97103) v2cpiwmbB145
CSPI02G01300Bhi02G000923Wax gourdcpiwgoB214
CSPI02G01300Cucsa.235690Cucumber (Gy14) v1cgycpiB349
CSPI02G01300CmaCh05G013280Cucurbita maxima (Rimu)cmacpiB800
CSPI02G01300CmaCh10G006010Cucurbita maxima (Rimu)cmacpiB073
CSPI02G01300CmoCh10G006410Cucurbita moschata (Rifu)cmocpiB066
CSPI02G01300Cp4.1LG04g09820Cucurbita pepo (Zucchini)cpecpiB665
CSPI02G01300Cp4.1LG18g04420Cucurbita pepo (Zucchini)cpecpiB370
CSPI02G01300Carg10872Silver-seed gourdcarcpiB0603
CSPI02G01300Carg09867Silver-seed gourdcarcpiB0143
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CSPI02G01300CSPI01G19440Wild cucumber (PI 183967)cpicpiB009
The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G01300Silver-seed gourdcarcpiB0044
CSPI02G01300Silver-seed gourdcarcpiB0308
CSPI02G01300Cucumber (Chinese Long) v3cpicucB080
CSPI02G01300Watermelon (97103) v2cpiwmbB105
CSPI02G01300Wax gourdcpiwgoB131
CSPI02G01300Cucumber (Gy14) v1cgycpiB152
CSPI02G01300Cucurbita maxima (Rimu)cmacpiB116
CSPI02G01300Cucurbita maxima (Rimu)cmacpiB174
CSPI02G01300Cucurbita moschata (Rifu)cmocpiB108
CSPI02G01300Cucurbita moschata (Rifu)cmocpiB159
CSPI02G01300Cucurbita moschata (Rifu)cmocpiB787
CSPI02G01300Melon (DHL92) v3.5.1cpimeB140
CSPI02G01300Watermelon (Charleston Gray)cpiwcgB102
CSPI02G01300Watermelon (Charleston Gray)cpiwcgB151
CSPI02G01300Watermelon (97103) v1cpiwmB165
CSPI02G01300Watermelon (97103) v1cpiwmB171
CSPI02G01300Cucurbita pepo (Zucchini)cpecpiB100
CSPI02G01300Cucurbita pepo (Zucchini)cpecpiB822
CSPI02G01300Bottle gourd (USVL1VR-Ls)cpilsiB096
CSPI02G01300Bottle gourd (USVL1VR-Ls)cpilsiB130
CSPI02G01300Melon (DHL92) v3.6.1cpimedB108
CSPI02G01300Melon (DHL92) v3.6.1cpimedB133
CSPI02G01300Cucumber (Gy14) v2cgybcpiB056