CSPI01G33100 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G33100
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	DNA-directed RNA polymerase subunit beta
Location	Chr1 : 27995025 .. 27998508 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGATTGTCTCCTGGAACACTAGGGGTTTAAATGACTCTTCCAAATGTGCAGCCCTCAATAAGTTCATAAAAATCTGCAACCCTAATGTAGTTATGATTCTTTTTTTTTGAAACGGAGACAAGTACTTATTTATTAATATGAACTCAATGTACAAGAGATTTATACGAGCCATAAAGAAGTAGTAATCAAGGGAGTCCTAGAGGGATCGGGAGGCGCACCCAGACACCTCAATTAGGTTGACACCCCAATAGTGCCAAAGATCATTTCCCGAGCATTACCAACCAAAACAATAAAGAAGCAATAATAAAAGCATCCAGCATAGTACAAAGGTCGGACCTGAAAAAAACAAGAAACTCGGCTGTGGCTTGTTGTGTAGGTGGAGACTGTCAATCTTTATTATCCCTCTCGGCTGAAAAAGTGAATAATTTCGGCGATAATAGATTTTAGTCCATTGATCACCATGGCTGGTTCACTGTTAAGCCTCTTTCGGCTCACCTAGAAACTGCCTCCCCAAAGAAAATGCCATTTTGGAGTTTTATCAACAATTTTCTTGTGTAGGCGGAGATCCGGTATTTTCTGAATCCTTATGTAAGGAATTACAAAAGAAATTCTTTCAACAAAGATGTGAATTAAAGGATTTAGAAAAAAAAAATTGGATCAAAAGAAAATGCCATTTTGGAGTTTTATCAACAATTTTCTTGTGTAGGCGGAGATCCGGTATTTTCTGAATCCTTATGTAAGGAATTACAAAAGAAATTCTTTCAACAAAGATGTGAATTAGGAAGGATTGGTCGACGAAATCTGAACCAGAGACTTAATCTTGATATACCTGAGAACAATACATTTTTGTTACAGCGAGATATATTGGCAGCTGCGGATCATTTGAGTGGACTTAAATTTGGAATGGGTACACTTGACGTTATGAATCATTTGAAAAATAAACGGATTCGTTGATGAATAACCTTCTTTTTACAGCTGTATGGAAATCAAGCAGCCCACAGAGAATTAACATCTTGATTTGGATTATTGTTTTCGGGTCCCTCAATTGTTCAAAAATATCGCAAAAGAAATCCCCTAACAAATGCCTCCTTCCTTCGATCTGTGCACTACGTATGAAGGCTAGTGAAGATATTCCTCATCTTTTTATGACCAGCCCCGTTTTGTTCTCCTGCTAGGTTAGGATTTTTTCTTGTTCAATTTTGATTGGGTTTTTGATCGATCACTAAGTGCTAATGTGTTTCTACTTTTGTCAGACCCGATTTTACCCAAGAAATCACATCTAATATGGAAAATATGTCAAATGTTCTCCTCGCAGAATTATGGTTCGAGTGTCTATTGGGAGGTTGTTTTCTAGTATCTACTAATCAGTTAAAAGTTGTTTCATGTTAATAAATAATGGTTGATTTTAAAAACATGTTTGTGAATGCGTGTAAGAGCCCTAATATTCATATAAATATATACAACAATAAGGTCATTTAAAAACTTTTTTTATTTTTCTTCTTCATTTCTTGATATCCATGAGTTTACACACTGACTAATCTCATGGGACTATTTTCCTTCTTAATTACACTTTATTTATTTTTGGTTACTAAGTGGGATATTTTACTTTTAGGTTGCTGAGAAGACGGCCCTTTTACGACCTGTGGAACTAAAGCGTGTCCCACTTGCCTTGGAAGCAAGTGCTTTTCGGAGCAAGTTCCTTGACACCGATGAACTGATTAGCTACTGTAGCTGGTTTGCAGTAAGAATTTCTGCTTTGTTCACTAAAATAATAATAATATTATTATTGTTATTGCCAGAGACATTTATTAATTTTTCTTGCTCACTAGTGTTTTTATGTTTTAAAACCATAAAAGAAAAAAATATAATTGGCTTTGTCCGATTGTGTATCATGTCATTGTTGACAATTTTTTTTTTCTAGCAAAAGAAAGGTCTCTTTGTCTCTCTTCAAAGTGAAGAAGTGTTAATGGTGCTGTTGGAAACTTAAGTTGGGTAAAGTTTATTTGGAGGGCAACCTCTGCTTTTTCCCCATTTTGATAGGTTTTTCTCTGTTTCCCTGGGTTATTGTCACCGCCCACTAATTGAGGGGAGTTGAGATGTTTGTTGCCTTTCTTTTGTTTCTGACCAGGTGACTCGTCATGGGGGAAGGGATTCTAGACTTTGGATTCTGAGAGGAGTTGGGTGTCTGGCTTTAAGTGTATAATAAGATCTTTTGGGGGTTGAAAATAACTATGGAGCTTTGGGAGGCGATTAGATTTAGTTCGTGGGTGTCTGTTACTTTTGTCCTAGTATTTTCCTTGTGGTTGGTTGGTTTGGTGAGTTGCTTTGTAGCTTATTTTAGGTTCCTTCTTCTTCCCTTTTTTTGGTTGTGTAATTGGTCTTTTTGTAGTTTTTTCCTTCCTTTCCCCTAGATTTCATTCGTTGATTTTTTTGGTCTTTTTTCTTCTAGAGTTTGTTTATTGAACATTTTGTTATTTTTCATATATCAATTAAAACCGTTATGCATTGTAATTGAGCTGTTAATCAATATACTTTTACATGGTGCTACTTTTTTGACGATTATTGGCAAGCGGAAGAAAAAAAGAATCAGAAGATGTATTTCCTATTTTCATTGTTTTAGTGGTATATATTCACATTTCCAACTGTCGATTTTCTTGATGATATCATTTATTGTCATTGAGCTTGAAAGCTACAATATGCTTAACATTAAGTAAATAATCACTTCATTCAATTTTTCTTTCTTTTTTATTTGAGAATTTATTTTTCTTGATTGATCTGTGTAAAATCTTTGTTATACAGACTTTCAGTGGTGTTGTTCCTGAGTGGGTGCAGAAAACCAGAATAGTAAAAAAACTAAACAAAATGTTGGTAAATCATCTGGGATTAACACTGTCTAAAGAAGATCTTCAAAATGTGGTTGATCTGATGGAACCATACGGTCAAATAAGCAATGGAATTGAACTTCTCAATCCTCCTCTTGATGTAAGAAACTACTTCACATTGGTTTTTAAGTTTTTAGCTGTGTTAGTTAGTTATTGTCATCTTAAAGTTGTAGGTTTAGTATTTTTTATATATCCAGTTGCAATTCATTACTTGACTATTACAAATTTTCAAGTATTTAAAGATTACTTTTTGATGCATAAAAGGATGAAGAAACATTAATGACGTACAGTCAACTTATGAAAAGTTGAGTTTGGATAGATAAGGGTATAGGATTACTTTAGTTTTTTTATTGTTCTCAGCTATGTTAAAAAGAAAAATGGTTACTGTTGTGTACAGTTTTTTGTTTGCATGGTAGTTATTAGCTGAAAAACGTTTTTTTATGAGACACAAGAGATATTATTGCTGAAAAACATTGGATTAAATTATAAGTCTAGTCCCGGATGAATTTCAGGATCATGTTGAGTTGGTCCTTGAACTCAATAGTGTACCGTGTTCCTAAATCCTTAATTTTATGTTTAATAGGTCTCTAAAGTTTAA

mRNA sequence

ATGAAGATTGTCTCCTGGAACACTAGGGGTTTAAATGACTCTTCCAAATGTGCAGCCCTCAATAAGTTCATAAAAATCTGCAACCCTAATGTGGAGACTGTCAATCTTTATTATCCCTCTCGGCTGAAAAAAAACTGCCTCCCCAAAGAAAATGCCATTTTGGAGTTTTATCAACAATTTTCTTGTGTAGGCGGAGATCCGGTATTTTCTGAATCCTTATGCGGAGATCCGGTATTTTCTGAATCCTTATGTAAGGAATTACAAAAGAAATTCTTTCAACAAAGATGTGAATTAGGAAGGATTGGTCGACGAAATCTGAACCAGAGACTTAATCTTGATATACCTGAGAACAATACATTTTTGTTACAGCGAGATATATTGGCAGCTGCGGATCATTTGAGTGGACTTAAATTTGGAATGGTATCTACTAATCAGTTAAAAGTTGCTGAGAAGACGGCCCTTTTACGACCTGTGGAACTAAAGCGTGTCCCACTTGCCTTGGAAGCAAGTGCTTTTCGGAGCAAGTTCCTTGACACCGATGAACTGATTAGCTACTGTAGCTGGTTTGCAACTTTCAGTGGTGTTGTTCCTGAGTGGGTGCAGAAAACCAGAATAGTAAAAAAACTAAACAAAATGTTGGTAAATCATCTGGGATTAACACTGTCTAAAGAAGATCTTCAAAATGTGGTTGATCTGATGGAACCATACGGTCAAATAAGCAATGGAATTGAACTTCTCAATCCTCCTCTTGATGTCTCTAAAGTTTAA

Coding sequence (CDS)

ATGAAGATTGTCTCCTGGAACACTAGGGGTTTAAATGACTCTTCCAAATGTGCAGCCCTCAATAAGTTCATAAAAATCTGCAACCCTAATGTGGAGACTGTCAATCTTTATTATCCCTCTCGGCTGAAAAAAAACTGCCTCCCCAAAGAAAATGCCATTTTGGAGTTTTATCAACAATTTTCTTGTGTAGGCGGAGATCCGGTATTTTCTGAATCCTTATGCGGAGATCCGGTATTTTCTGAATCCTTATGTAAGGAATTACAAAAGAAATTCTTTCAACAAAGATGTGAATTAGGAAGGATTGGTCGACGAAATCTGAACCAGAGACTTAATCTTGATATACCTGAGAACAATACATTTTTGTTACAGCGAGATATATTGGCAGCTGCGGATCATTTGAGTGGACTTAAATTTGGAATGGTATCTACTAATCAGTTAAAAGTTGCTGAGAAGACGGCCCTTTTACGACCTGTGGAACTAAAGCGTGTCCCACTTGCCTTGGAAGCAAGTGCTTTTCGGAGCAAGTTCCTTGACACCGATGAACTGATTAGCTACTGTAGCTGGTTTGCAACTTTCAGTGGTGTTGTTCCTGAGTGGGTGCAGAAAACCAGAATAGTAAAAAAACTAAACAAAATGTTGGTAAATCATCTGGGATTAACACTGTCTAAAGAAGATCTTCAAAATGTGGTTGATCTGATGGAACCATACGGTCAAATAAGCAATGGAATTGAACTTCTCAATCCTCCTCTTGATGTCTCTAAAGTTTAA

Homology

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BLAST of CSPI01G33100 vs. Swiss-Prot
Match: FTSI5_ARATH (Probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic OS=Arabidopsis thaliana GN=FTSHI5 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.2e-41
Identity = 80/105 (76.19%), Postives = 95/105 (90.48%), Query Frame = 1


Query: 147  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 206
            KV+EKT LLRP+ELK VP+ALE+SAFRSKFLDTDEL+SY SWFATFS +VP W++KT++ 
Sbjct: 984  KVSEKTTLLRPIELKLVPMALESSAFRSKFLDTDELLSYVSWFATFSHIVPPWLRKTKVA 1043

Query: 207  KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 252
            K + KMLVNHLGL L+K+DL+NVVDLMEPYGQISNGIELLNP +D
Sbjct: 1044 KTMGKMLVNHLGLNLTKDDLENVVDLMEPYGQISNGIELLNPTVD 1088

[Close]

BLAST of CSPI01G33100 vs. Swiss-Prot
Match: RPOB_CUCSA (DNA-directed RNA polymerase subunit beta OS=Cucumis sativus GN=rpoB PE=3 SV=2)

HSP 1 Score: 161.8 bits (408), Expect = 1.0e-38
Identity = 86/115 (74.78%), Postives = 89/115 (77.39%), Query Frame = 1


Query: 26  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCK 85
           +C P  E    +   + KK    KENAILEFYQQFSCVG          GDPVFSESLCK
Sbjct: 198 VCYP--EIFLSFLNDKEKKKIGSKENAILEFYQQFSCVG----------GDPVFSESLCK 257

Query: 86  ELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLSGLKFGM 141
           ELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHL GLKFGM
Sbjct: 258 ELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLIGLKFGM 300

[Close]

BLAST of CSPI01G33100 vs. Swiss-Prot
Match: RPOB_AMBTC (DNA-directed RNA polymerase subunit beta OS=Amborella trichopoda GN=rpoB PE=3 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 3.3e-37
Identity = 88/169 (52.07%), Postives = 108/169 (63.91%), Query Frame = 1


Query: 26  ICNPNVETVNLYYPS-RLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLC 85
           +C P +    LY+P+ + KK    KENAILEFYQQFSCVG          GDPVFSESLC
Sbjct: 198 VCYPEIL---LYFPNEKEKKKIGSKENAILEFYQQFSCVG----------GDPVFSESLC 257

Query: 86  KELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLSGLKFGMVSTN 145
           KELQK+FFQQRCELGRIGR+N+NQRLN+DIP+NNTFLL RD+LAA DHL G+KFGM + +
Sbjct: 258 KELQKRFFQQRCELGRIGRQNMNQRLNIDIPQNNTFLLPRDVLAATDHLIGMKFGMGTLD 317

Query: 146 QLKVAEKTAL------------LRPVELKRVPLALEASAFRSKFLDTDE 182
            +   +   +            L  V L+ V       A R KF+ T +
Sbjct: 318 DMNHLKNKRIRSVADLLQDQFGLALVRLENVVRGTICGAIRHKFIPTPQ 353

[Close]

BLAST of CSPI01G33100 vs. Swiss-Prot
Match: RPOB_ILLOL (DNA-directed RNA polymerase subunit beta OS=Illicium oligandrum GN=rpoB PE=3 SV=2)

HSP 1 Score: 154.5 bits (389), Expect = 1.6e-36
Identity = 80/122 (65.57%), Postives = 94/122 (77.05%), Query Frame = 1


Query: 26  ICNPNVETVNLYYPS-RLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLC 85
           +C P +    L +P+ + KK    KENA+LEFYQQF+CVG          GDPVFSESLC
Sbjct: 198 VCYPEIF---LSFPNDKEKKKIGSKENAVLEFYQQFACVG----------GDPVFSESLC 257

Query: 86  KELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLSGLKFGMVSTN 145
           KELQKKFFQQRCELGRIGRRN+NQRLNLDIP+N+TFLL RD+LAAADHL G+KFGM + +
Sbjct: 258 KELQKKFFQQRCELGRIGRRNMNQRLNLDIPQNSTFLLPRDVLAAADHLIGMKFGMGTLD 306

Query: 146 QL 147
            L
Sbjct: 318 DL 306

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BLAST of CSPI01G33100 vs. Swiss-Prot
Match: RPOB_LEMMI (DNA-directed RNA polymerase subunit beta OS=Lemna minor GN=rpoB PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.6e-36
Identity = 80/115 (69.57%), Postives = 87/115 (75.65%), Query Frame = 1


Query: 26  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCK 85
           +C P  E    +   + KK    KENAILEFYQQF+CVG          GDPVFSESLCK
Sbjct: 198 VCYP--EIFLSFLNDKEKKKIGSKENAILEFYQQFACVG----------GDPVFSESLCK 257

Query: 86  ELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLSGLKFGM 141
           ELQKKFFQQRCELGRIGRRN+NQRLNLDIP NNTFLL RD+LAAADHL G+KFGM
Sbjct: 258 ELQKKFFQQRCELGRIGRRNINQRLNLDIPHNNTFLLPRDVLAAADHLIGMKFGM 300

[Close]

BLAST of CSPI01G33100 vs. TrEMBL
Match: A0A0A0M3D4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G673530 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.0e-98
Identity = 192/224 (85.71%), Postives = 198/224 (88.39%), Query Frame = 1


Query: 29  PNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQ 88
           P+++  ++Y      KN  PK    + FYQQFSCVG          GDPVFSESLCKELQ
Sbjct: 9   PSIKAFSMYD----LKNFTPKR---ISFYQQFSCVG----------GDPVFSESLCKELQ 68

Query: 89  KKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLSGLKFGMVSTNQLKV 148
           KKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHL GLKFGMVSTNQLKV
Sbjct: 69  KKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLIGLKFGMVSTNQLKV 128

Query: 149 AEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKK 208
           AEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKK
Sbjct: 129 AEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKK 188

Query: 209 LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLDV 253
           LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLDV
Sbjct: 189 LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLDV 215

[Close]

BLAST of CSPI01G33100 vs. TrEMBL
Match: W9RHH7_9ROSA (ATP-dependent zinc metalloprotease FtsH OS=Morus notabilis GN=L484_024479 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.9e-44
Identity = 97/139 (69.78%), Postives = 110/139 (79.14%), Query Frame = 1


Query: 120  FLLQRDILAAADHLSGLKFGMVSTNQL-------KVAEKTALLRPVELKRVPLALEASAF 179
            F LQR   A  + +  +       N+L       KVAEKTALLRP+ELK VP+ALE SAF
Sbjct: 933  FHLQRPTQAEREKILQIAAKETMDNELIDFVDWKKVAEKTALLRPIELKLVPVALEGSAF 992

Query: 180  RSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDL 239
            RSKFLD DEL+SYC WFATFSG +P W++KT+IVKKL+KMLVNHLGLTL+KEDLQNVVDL
Sbjct: 993  RSKFLDMDELMSYCGWFATFSGFIPGWLRKTKIVKKLSKMLVNHLGLTLTKEDLQNVVDL 1052

Query: 240  MEPYGQISNGIELLNPPLD 252
            MEPYGQISNGIELLNPPLD
Sbjct: 1053 MEPYGQISNGIELLNPPLD 1071

[Close]

BLAST of CSPI01G33100 vs. TrEMBL
Match: B9I5Q6_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s04620g PE=4 SV=2)

HSP 1 Score: 187.2 bits (474), Expect = 2.5e-44
Identity = 90/105 (85.71%), Postives = 101/105 (96.19%), Query Frame = 1


Query: 147  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 206
            KVAEKTALLRPVELK VP+ALE SAF+SKFLDTDEL+SYCSWFATFS +VP+WV+KT+I 
Sbjct: 969  KVAEKTALLRPVELKLVPVALEGSAFKSKFLDTDELMSYCSWFATFSCLVPDWVRKTKIA 1028

Query: 207  KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 252
            KK+++M+VNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD
Sbjct: 1029 KKMSRMMVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 1073

[Close]

BLAST of CSPI01G33100 vs. TrEMBL
Match: A0A067EDF6_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g0355612mg PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 7.3e-44
Identity = 89/105 (84.76%), Postives = 100/105 (95.24%), Query Frame = 1


Query: 147 KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 206
           KVAEKTALLRP+ELK VP+ALE SAFRSKFLDTDEL+SYC WFATFSGVVP+W +KT+IV
Sbjct: 495 KVAEKTALLRPIELKLVPVALEGSAFRSKFLDTDELMSYCGWFATFSGVVPKWFRKTKIV 554

Query: 207 KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 252
           KK+++MLV+HLGLTL+KEDLQNVVDLMEPYGQISNGIELL PPLD
Sbjct: 555 KKISRMLVDHLGLTLTKEDLQNVVDLMEPYGQISNGIELLTPPLD 599

[Close]

BLAST of CSPI01G33100 vs. TrEMBL
Match: V4VE66_9ROSI (Uncharacterized protein (Fragment) OS=Citrus clementina GN=CICLE_v100305692mg PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 7.3e-44
Identity = 89/105 (84.76%), Postives = 100/105 (95.24%), Query Frame = 1


Query: 147 KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 206
           KVAEKTALLRP+ELK VP+ALE SAFRSKFLDTDEL+SYC WFATFSGVVP+W +KT+IV
Sbjct: 23  KVAEKTALLRPIELKLVPVALEGSAFRSKFLDTDELMSYCGWFATFSGVVPKWFRKTKIV 82

Query: 207 KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 252
           KK+++MLV+HLGLTL+KEDLQNVVDLMEPYGQISNGIELL PPLD
Sbjct: 83  KKISRMLVDHLGLTLTKEDLQNVVDLMEPYGQISNGIELLTPPLD 127

[Close]

BLAST of CSPI01G33100 vs. TAIR10
Match: AT3G04340.1 (AT3G04340.1 FtsH extracellular protease family)

HSP 1 Score: 170.6 bits (431), Expect = 1.2e-42
Identity = 80/105 (76.19%), Postives = 95/105 (90.48%), Query Frame = 1


Query: 147  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 206
            KV+EKT LLRP+ELK VP+ALE+SAFRSKFLDTDEL+SY SWFATFS +VP W++KT++ 
Sbjct: 984  KVSEKTTLLRPIELKLVPMALESSAFRSKFLDTDELLSYVSWFATFSHIVPPWLRKTKVA 1043

Query: 207  KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 252
            K + KMLVNHLGL L+K+DL+NVVDLMEPYGQISNGIELLNP +D
Sbjct: 1044 KTMGKMLVNHLGLNLTKDDLENVVDLMEPYGQISNGIELLNPTVD 1088

[Close]

BLAST of CSPI01G33100 vs. TAIR10
Match: ATCG00190.1 (ATCG00190.1 RNA polymerase subunit beta)

HSP 1 Score: 147.1 bits (370), Expect = 1.5e-35
Identity = 79/142 (55.63%), Postives = 95/142 (66.90%), Query Frame = 1


Query: 26  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCK 85
           +C P  E    +   + KK    KENAILEFYQQFSCVG          GDP+FSESLCK
Sbjct: 198 VCYP--EIFLSFLTDKEKKKIGSKENAILEFYQQFSCVG----------GDPIFSESLCK 257

Query: 86  ELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLSGLKFGMVSTNQ 145
           ELQKKFF QRCELGRIGRRN+N RLNL+IP+NN FLL RD+LAAADHL G+KFGM + + 
Sbjct: 258 ELQKKFFHQRCELGRIGRRNINWRLNLNIPQNNIFLLPRDVLAAADHLIGMKFGMGTLDD 317

Query: 146 LKVAEKTALLRPVELKRVPLAL 168
           +   +   +    +L +  L L
Sbjct: 318 MNHLKNKRIRSVADLLQDQLGL 327

[Close]

BLAST of CSPI01G33100 vs. NCBI nr
Match: gi|700211650|gb|KGN66746.1| (hypothetical protein Csa_1G673530 [Cucumis sativus])

HSP 1 Score: 367.9 bits (943), Expect = 1.5e-98
Identity = 192/224 (85.71%), Postives = 198/224 (88.39%), Query Frame = 1


Query: 29  PNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQ 88
           P+++  ++Y      KN  PK    + FYQQFSCVG          GDPVFSESLCKELQ
Sbjct: 9   PSIKAFSMYD----LKNFTPKR---ISFYQQFSCVG----------GDPVFSESLCKELQ 68

Query: 89  KKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLSGLKFGMVSTNQLKV 148
           KKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHL GLKFGMVSTNQLKV
Sbjct: 69  KKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILAAADHLIGLKFGMVSTNQLKV 128

Query: 149 AEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKK 208
           AEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKK
Sbjct: 129 AEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKK 188

Query: 209 LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLDV 253
           LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLDV
Sbjct: 189 LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLDV 215

[Close]

BLAST of CSPI01G33100 vs. NCBI nr
Match: gi|449449669|ref|XP_004142587.1| (PREDICTED: uncharacterized protein LOC101207174 [Cucumis sativus])

HSP 1 Score: 209.5 bits (532), Expect = 6.8e-51
Identity = 105/105 (100.00%), Postives = 105/105 (100.00%), Query Frame = 1


Query: 147  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 206
            KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV
Sbjct: 991  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 1050

Query: 207  KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 252
            KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD
Sbjct: 1051 KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 1095

[Close]

BLAST of CSPI01G33100 vs. NCBI nr
Match: gi|703120875|ref|XP_010102198.1| (ATP-dependent zinc metalloprotease FtsH [Morus notabilis])

HSP 1 Score: 187.6 bits (475), Expect = 2.8e-44
Identity = 97/139 (69.78%), Postives = 110/139 (79.14%), Query Frame = 1


Query: 120  FLLQRDILAAADHLSGLKFGMVSTNQL-------KVAEKTALLRPVELKRVPLALEASAF 179
            F LQR   A  + +  +       N+L       KVAEKTALLRP+ELK VP+ALE SAF
Sbjct: 933  FHLQRPTQAEREKILQIAAKETMDNELIDFVDWKKVAEKTALLRPIELKLVPVALEGSAF 992

Query: 180  RSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDL 239
            RSKFLD DEL+SYC WFATFSG +P W++KT+IVKKL+KMLVNHLGLTL+KEDLQNVVDL
Sbjct: 993  RSKFLDMDELMSYCGWFATFSGFIPGWLRKTKIVKKLSKMLVNHLGLTLTKEDLQNVVDL 1052

Query: 240  MEPYGQISNGIELLNPPLD 252
            MEPYGQISNGIELLNPPLD
Sbjct: 1053 MEPYGQISNGIELLNPPLD 1071

[Close]

BLAST of CSPI01G33100 vs. NCBI nr
Match: gi|743888496|ref|XP_011038463.1| (PREDICTED: uncharacterized protein LOC105135337 [Populus euphratica])

HSP 1 Score: 187.2 bits (474), Expect = 3.6e-44
Identity = 90/105 (85.71%), Postives = 101/105 (96.19%), Query Frame = 1


Query: 147  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 206
            KVAEKTALLRPVELK VP+ALE SAF+SKFLDTDEL+SYCSWFATFS +VP+WV+KT+I 
Sbjct: 969  KVAEKTALLRPVELKLVPVALEGSAFKSKFLDTDELMSYCSWFATFSCLVPDWVRKTKIA 1028

Query: 207  KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 252
            KK+++M+VNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD
Sbjct: 1029 KKMSRMMVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 1073

[Close]

BLAST of CSPI01G33100 vs. NCBI nr
Match: gi|566199445|ref|XP_002319118.2| (hypothetical protein POPTR_0013s04620g [Populus trichocarpa])

HSP 1 Score: 187.2 bits (474), Expect = 3.6e-44
Identity = 90/105 (85.71%), Postives = 101/105 (96.19%), Query Frame = 1


Query: 147  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIV 206
            KVAEKTALLRPVELK VP+ALE SAF+SKFLDTDEL+SYCSWFATFS +VP+WV+KT+I 
Sbjct: 969  KVAEKTALLRPVELKLVPVALEGSAFKSKFLDTDELMSYCSWFATFSCLVPDWVRKTKIA 1028

Query: 207  KKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 252
            KK+++M+VNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD
Sbjct: 1029 KKMSRMMVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELLNPPLD 1073

The following BLAST results are available for this feature:

BLAST of CSPI01G33100 vs. Swiss-Prot


Match Name	E-value	Identity	Description
FTSI5_ARATH	2.2e-41	76.19	Probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic OS=A...	[more]
RPOB_CUCSA	1.0e-38	74.78	DNA-directed RNA polymerase subunit beta OS=Cucumis sativus GN=rpoB PE=3 SV=2	[more]
RPOB_AMBTC	3.3e-37	52.07	DNA-directed RNA polymerase subunit beta OS=Amborella trichopoda GN=rpoB PE=3 SV...	[more]
RPOB_ILLOL	1.6e-36	65.57	DNA-directed RNA polymerase subunit beta OS=Illicium oligandrum GN=rpoB PE=3 SV=...	[more]
RPOB_LEMMI	1.6e-36	69.57	DNA-directed RNA polymerase subunit beta OS=Lemna minor GN=rpoB PE=3 SV=1	[more]

BLAST of CSPI01G33100 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M3D4_CUCSA	1.0e-98	85.71	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G673530 PE=3 SV=1	[more]
W9RHH7_9ROSA	1.9e-44	69.78	ATP-dependent zinc metalloprotease FtsH OS=Morus notabilis GN=L484_024479 PE=4 S...	[more]
B9I5Q6_POPTR	2.5e-44	85.71	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s04620g PE=4 SV=2	[more]
A0A067EDF6_CITSI	7.3e-44	84.76	Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g0355612mg PE=4 ...	[more]
V4VE66_9ROSI	7.3e-44	84.76	Uncharacterized protein (Fragment) OS=Citrus clementina GN=CICLE_v100305692mg PE...	[more]

BLAST of CSPI01G33100 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G04340.1	1.2e-42	76.19	FtsH extracellular protease family	[more]
ATCG00190.1	1.5e-35	55.63	ATCG00190.1 RNA polymerase subunit beta	[more]

BLAST of CSPI01G33100 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700211650\|gb\|KGN66746.1\|	1.5e-98	85.71	hypothetical protein Csa_1G673530 [Cucumis sativus]	[more]
gi\|449449669\|ref\|XP_004142587.1\|	6.8e-51	100.00	PREDICTED: uncharacterized protein LOC101207174 [Cucumis sativus]	[more]
gi\|703120875\|ref\|XP_010102198.1\|	2.8e-44	69.78	ATP-dependent zinc metalloprotease FtsH [Morus notabilis]	[more]
gi\|743888496\|ref\|XP_011038463.1\|	3.6e-44	85.71	PREDICTED: uncharacterized protein LOC105135337 [Populus euphratica]	[more]
gi\|566199445\|ref\|XP_002319118.2\|	3.6e-44	85.71	hypothetical protein POPTR_0013s04620g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR007642	RNA_pol_Rpb2_2

Vocabulary: **Biological Process**
Term	Definition
GO:0006351	transcription, DNA-templated

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003899	DNA-directed RNA polymerase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0009506	plasmodesma
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0004222	metalloendopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G33100.1	CSPI01G33100.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007642	RNA polymerase Rpb2, domain 2	GENE3D	G3DSA:3.90.1110.10		coord: 74..140 score: 2.
IPR007642	RNA polymerase Rpb2, domain 2	PFAM	PF04561	RNA_pol_Rpb2_2	coord: 46..142 score: 1.8
None	No IPR available	PANTHER	PTHR23076	METALLOPROTEASE M41 FTSH	coord: 211..251 score: 1.2
None	No IPR available	PANTHER	PTHR23076:SF58	FTSH EXTRACELLULAR PROTEASE FAMILY PROTEIN	coord: 211..251 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G33100	Cla000624	Watermelon (97103) v1	cpiwmB020
CSPI01G33100	Csa1G673530	Cucumber (Chinese Long) v2	cpicuB001
CSPI01G33100	Cucsa.027450	Cucumber (Gy14) v1	cgycpiB584
CSPI01G33100	CsGy1G031890	Cucumber (Gy14) v2	cgybcpiB002

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G33100	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G33100	Melon (DHL92) v3.5.1	cpimeB044
CSPI01G33100	Bottle gourd (USVL1VR-Ls)	cpilsiB059
CSPI01G33100	Melon (DHL92) v3.6.1	cpimedB038