CSPI01G26670 (gene) Wild cucumber (PI 183967)

NameCSPI01G26670
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionIsoaspartyl peptidase/L-asparaginase
LocationChr1 : 21761490 .. 21765033 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGATAGTTGGATCGATTTTATCTGTTTCGGGTGAAACAAAAGATAACTCAATATTAATACAAACTATATATAATTTGGCAACCCAAAGAGAGAATGGAGAGGTTGCACCAACTCCACTATATAAAATTTCACAATCCTTCACTATAAGAAGTCTCGCCTATCGCCGGCGAATGATCCCAATAATCCTTTAAGCTAAGGATGGGATGGGCAATTGCTCTTCACGGCGGAGCCGGCGACATTCCCCTCTCATTACCATCCGACCGCCGCCAACCGCGAGAGGAAACCCTCCGCCGTTGCCTGCAAATCGGCGTTCAAGCTCTCAAATCTCAGAAGCCTCCTTTGGATGTTGTTGAACTTGTGGTGAGAAATTTTTACTCTCGAGTTTGATTTGCCTTAAAGTGAAATCAAATATGTCGTTTATGTAATCTAATCTGTAATATGTAAAATTTTTACTCTCTTTTTTTTTTTTTTTTTTTGTTATTGTTGGTGGACTCTTGCGTTAAATATGAGCTTTAGAATCTAATGATTTACTGTGTGGAATTTTCAAGTTTGTCTTAATTTAGTTCGAGATGGAGTGTTGAATTTGTAGTGATTTGGTCTCTAATGGGAGAAATTTTGATGAGTGATGGGGTTTTGTAATTAGTGACCAAATACAATTTTTCATGGAGGATAATGGGTTGTTACTGATTTTAATCCACGGATTAATTTTTTTACATTTCTGAGGGTTTGATCAATGGGAATATTAAATTACAATGGCTTTGAACTAAATAAACTTTTCTGTTTTATGTCTTTTAGCTTTCTTTTCTTGAACTTCGGAAGTTTTTCCATGTTCTTTCTTTTTTGTTTCCTTTTCTTTCTGCCTGCTGAAACTCAGTGTATGTGGGTCCTTGCTTTTCTGCAAATGGGAAATTGTGTATACTTTCGTTTGGGGATTTGAGTTTTGATGTTATAACCTAGGCTAAGAAGCATACACTGACATGCGAACACACACTAATAAGCACAGACGCTGACATGCAAACAGACACTCATACAGCCACATGCAAATTTTTTAAAAAAAGTAGACCACTGCAAGTGGGAACATGCTTTTTTTTAGAACAAAAATAAATATGTGTGCAGACTTGACATTTTGTGAAATAGGCACTTATACATTTAACGAAAGAAAAAATTAGGAAGATAAAAGTGGAAGGACAAATAACAGAAATGAGAAAAGAAAGAAATATAGTAAGAGAAGTTGCCAGCAGACGTGAATCTTGAAGTTGAAGTACTCGTTGTTGATAAAGTTAGAAAGAAGAATCGGGCAGCAGTGTTTTTTTTATTTTTTTCCTGAAAAATCCAGAATATGAATTAAGTGACTTGAGTCTTAGGGTGAAGTGTTTTACTGTGCAAAAAGTGTCCATAGTTCCATTTCAATGGGCTTTAAGTGCCTTTTAAATGGGTTGTCATTGCCAATAGTAGGCATTGTTTCATTTTTTAAAATTCCTTTTCTCCTTTGTTTTTTGTAGATGGTGTGTCCTATCGTGTTAGAAAGCAAAATAGTAATAATAAAATAATAGAGACACCAGGTGACGTGTCGGACACATCACTGACGCTCGGCCATTTTAGGGGTGTTGGTCCTTTATAGGTTATAACCAAACATTTGCTTTTTAAATGTCAACAAGAATGTTTTTTCAGCTCATTAATTAATGACTTATTAGTGTGGAAATGGATTATTGACGGGAAATTAGAGAAACTACTGAAGGGGTGTTTGCCCTATGGTTTAATCAGCCATTTCTGAAATTTTTGGACGAAAATTTGAATTGTATAAGTTCTTGTAATATATATTCTTAGTAAAAGATAACGACAGACTTAGGTGAGTTTCATAAGTCTATATATATTTTATCTTGTATAATTTATTTTGACTTGATGTAAGATGGTTCCGTGTTGAATTTGTTCTTGTACAGGTACGAGAACTCGAAAATTGTCCACATTTCAATGCAGGTAGGGGGTCTGTCTTAACAACCAAGGCAACCGTAGAAATGGAAGCTAGCATCATGGACAGTACGAAGCGATGTGGAGCTGTTTCGGGTCTCACCACTGTCGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAAGCTTTTGCAAGGGAACAGGTTGGGAATTTCTATCCCTCTTTCTATGTTACTGTAGAATTAATTCTCTTTTTATACATTATATATTATTGCACAATTATAGATAGATTCATTGTGATTCATGACTGTTTTATTTGGATCAATTTTTCTGAAAAATGTCAAGGGAGTGGAGACCATTGATTCAAGCTACTTTATCACACCACAGAACATTGAAAGGCTACAACAAGCTAAAGAGGCAAACCGAGTGCAGGTTTTCTGCTTATCTTTTATCCCAATACATGAATTAACTGCTCTCTTTTTAAGCCTCTCTAAACTTACACGTATGTTATATTGTTAAGCTAGATCAATTGTGCCAACTTCTAGGCATTTTCACATTTTAATTTTATAGAAGTGTTTAGAGTGATATTTATAAGGAATTTAAGATAAAAAAGTGTAGAAGTGAGACAAATTGGAATTCTACACTTTTTGAATGAGTTAGATGTTATTAATACTACATTTACGTTTTGTTTATATACTTAATGTTTTGTTCATCAGTGTTGATTCTCTGTTTTTAACTCTTGTAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAATCCAAGTGGTGATAGTCAAATAGGAACTGTTGGATGTGTGGCTGTTGATAACCATGGAAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGTGACACTCCGTTAATAGGAGCTGGCACTTATGCTAACCATCTCTGTGCAATTTCTGCAACCGGAAAGGGTGAATCCATCATACGCAGTACCGTGGCGAGGGATGTGGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTAGAAGAAGCAGCTGCCTCTGTTGTTGAAAGTGTTCCTAAAGGCAATGTCGGTTTGATCGCCGTATCTGCCTCTGGCGAAGTTGCGATGCCTTTTAATACGACTGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTATTCAGAGATTTCCATATGGTCTTCTAAACATGATTGAATGAAAACTGTTGTGAGAAAGCCATAGCTGAGATTTGGTTATATTTTCTGTTGTTATGGTTTGGTGTCTCCCAGCATATGTAAGAATATTCTTCCAAAATGATCTTCCATTTCATGTCTTCGGTTGTTTTCAGTGGGAAGTCGTTCTCAATTATGTTCAATAAGGTTGTTCTGTAATATACTATTAAGTTTAGTGTTTTGAAAGTATTTGTAATGTAGAGCTTAAAAAAGGACACGTCAAACGAGAAACATAGCAACAAATAAAGATTGTCTTTAGCACACCTTCGTCGTTATCGTTGGGTCATGCCAACTCCCTTTGTAGCATACTCTTTCTCAATCCTCACGTTTCAT

mRNA sequence

ATGGGATGGGCAATTGCTCTTCACGGCGGAGCCGGCGACATTCCCCTCTCATTACCATCCGACCGCCGCCAACCGCGAGAGGAAACCCTCCGCCGTTGCCTGCAAATCGGCGTTCAAGCTCTCAAATCTCAGAAGCCTCCTTTGGATGTTGTTGAACTTGTGGTACGAGAACTCGAAAATTGTCCACATTTCAATGCAGGTAGGGGGTCTGTCTTAACAACCAAGGCAACCGTAGAAATGGAAGCTAGCATCATGGACAGTACGAAGCGATGTGGAGCTGTTTCGGGTCTCACCACTGTCGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAAGCTTTTGCAAGGGAACAGGGAGTGGAGACCATTGATTCAAGCTACTTTATCACACCACAGAACATTGAAAGGCTACAACAAGCTAAAGAGGCAAACCGAGTGCAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAATCCAAGTGGTGATAGTCAAATAGGAACTGTTGGATGTGTGGCTGTTGATAACCATGGAAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGTGACACTCCGTTAATAGGAGCTGGCACTTATGCTAACCATCTCTGTGCAATTTCTGCAACCGGAAAGGGTGAATCCATCATACGCAGTACCGTGGCGAGGGATGTGGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTAGAAGAAGCAGCTGCCTCTGTTGTTGAAAGTGTTCCTAAAGGCAATGTCGGTTTGATCGCCGTATCTGCCTCTGGCGAAGTTGCGATGCCTTTTAATACGACTGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTATTCAGAGATTTCCATATGGTCTTCTAAACATGATTGA

Coding sequence (CDS)

ATGGGATGGGCAATTGCTCTTCACGGCGGAGCCGGCGACATTCCCCTCTCATTACCATCCGACCGCCGCCAACCGCGAGAGGAAACCCTCCGCCGTTGCCTGCAAATCGGCGTTCAAGCTCTCAAATCTCAGAAGCCTCCTTTGGATGTTGTTGAACTTGTGGTACGAGAACTCGAAAATTGTCCACATTTCAATGCAGGTAGGGGGTCTGTCTTAACAACCAAGGCAACCGTAGAAATGGAAGCTAGCATCATGGACAGTACGAAGCGATGTGGAGCTGTTTCGGGTCTCACCACTGTCGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAAGCTTTTGCAAGGGAACAGGGAGTGGAGACCATTGATTCAAGCTACTTTATCACACCACAGAACATTGAAAGGCTACAACAAGCTAAAGAGGCAAACCGAGTGCAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAATCCAAGTGGTGATAGTCAAATAGGAACTGTTGGATGTGTGGCTGTTGATAACCATGGAAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGTGACACTCCGTTAATAGGAGCTGGCACTTATGCTAACCATCTCTGTGCAATTTCTGCAACCGGAAAGGGTGAATCCATCATACGCAGTACCGTGGCGAGGGATGTGGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTAGAAGAAGCAGCTGCCTCTGTTGTTGAAAGTGTTCCTAAAGGCAATGTCGGTTTGATCGCCGTATCTGCCTCTGGCGAAGTTGCGATGCCTTTTAATACGACTGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTATTCAGAGATTTCCATATGGTCTTCTAAACATGATTGA
BLAST of CSPI01G26670 vs. Swiss-Prot
Match: ASPGA_ARATH (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 484.6 bits (1246), Expect = 8.5e-136
Identity = 251/316 (79.43%), Postives = 275/316 (87.03%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP+ LP +RR PRE  LR CL +G+ ALKS KPPLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDS-TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P FNAG+GSVLT + TVEMEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPS 180
           AFD AEAFAR  GVET+DSS+FITP+NI RL+QAKE NRVQ+DYT P P+  +       
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----C 181

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD+ GNLASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 ESIIRSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 300
           E IIR TVARDVAALME+KGLSL EAAA VV +SVP+G+ GL+AVSA+GEV MPFNTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEISIW 315
           FRACA+EDGYSEI+IW
Sbjct: 302 FRACASEDGYSEIAIW 312

BLAST of CSPI01G26670 vs. Swiss-Prot
Match: ASPG_LUPAN (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 2.5e-135
Identity = 254/320 (79.38%), Postives = 281/320 (87.81%), Query Frame = 1

Query: 2   GWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLP +RRQPREE LR CLQIGV+ALKSQKPPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKATVEMEASIMDS-TKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT   TVEMEASIMD  T +CGAVSGL+TV+NPISLARLVM+KTPHIYLA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQ---PLPQATEQETEN 181
           F GA+ FA++QGVET+DSS+FIT +N+ERL+ A EANRVQ+DY+Q   P P   + E E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEKEL 182

Query: 182 P--SGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
           P  +GDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGESIIRSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGE+II +TVARDVAALMEFKGLSL+EAA  VV E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEISIW 315
           TTGMFRA ATEDGYSEI+IW
Sbjct: 303 TTGMFRASATEDGYSEIAIW 322

BLAST of CSPI01G26670 vs. Swiss-Prot
Match: ASPG_LUPLU (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus PE=1 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 2.5e-135
Identity = 252/320 (78.75%), Postives = 282/320 (88.12%), Query Frame = 1

Query: 2   GWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLP +RR+PREE LR CLQIGV+ALK+QKPPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKATVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT   TVEMEASIMD +T +CGAVSGL+TV+NPISLARLVM+KTPHIYLA
Sbjct: 63  EHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQ---PLPQATEQETEN 181
           F GA+ FA++QGVET+DSS+ IT +N+ERL+ A EANRVQ+DY+Q   P P   + E E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEKEL 182

Query: 182 P--SGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
           P  +GDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLTNGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGESIIRSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGE IIR+TVARDVAALMEFKGLSL+EAA  V+ E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEISIW 315
           TTGMFRACATEDGYSEI+IW
Sbjct: 303 TTGMFRACATEDGYSEIAIW 322

BLAST of CSPI01G26670 vs. Swiss-Prot
Match: ASPG_LUPAL (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.0e-133
Identity = 247/320 (77.19%), Postives = 278/320 (86.88%), Query Frame = 1

Query: 2   GWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLP +RR+PREE LR CLQIG++ALK+Q PPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKATVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT   TVEMEASIMD +T +CGAVSGL TV+NPISLAR VM+KTPHI+LA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIFLA 122

Query: 122 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDY-----TQPLPQATEQET 181
           F GA+   ++QGVET+DSS+FIT +N+ERL+ A EANRVQ+DY     TQP+    E+E 
Sbjct: 123 FQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEKEL 182

Query: 182 ENPSGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
              +GDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGESIIRSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGE+IIR+TVARDVAALMEFKGLSL+EAA  VV E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEISIW 315
           TTGMFRACATEDGYSEI+IW
Sbjct: 303 TTGMFRACATEDGYSEIAIW 322

BLAST of CSPI01G26670 vs. Swiss-Prot
Match: ASPG_LUPAR (Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 4.1e-122
Identity = 233/303 (76.90%), Postives = 263/303 (86.80%), Query Frame = 1

Query: 19  PSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHFNAGRGSVLTTKATV 78
           P +RR+PREE LR CLQIGV+ALK++K PLDVVELVVRELEN  HFNAG GSVLT   TV
Sbjct: 1   PPERRKPREEGLRHCLQIGVEALKARKSPLDVVELVVRELENNEHFNAGIGSVLTNSGTV 60

Query: 79  EMEASIMDS-TKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETID 138
           EMEASIMD  + +CGAVSGL+TV+NPISLARLVMEKTPH+YLAF GA+ FA++QGVET+D
Sbjct: 61  EMEASIMDGKSMKCGAVSGLSTVLNPISLARLVMEKTPHMYLAFQGAQDFAKQQGVETVD 120

Query: 139 SSYFITPQNIERLQQAKEANRVQIDY-----TQPLPQATEQETENPSGDSQIGTVGCVAV 198
           SS+FIT +N+ERL+ A EANRVQIDY     TQP+    E+E    +GDSQIGTVGCVAV
Sbjct: 121 SSHFITAENVERLKLAIEANRVQIDYSQYNYTQPVQDDAEKELPVANGDSQIGTVGCVAV 180

Query: 199 DNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGESIIRSTVARDVA 258
           D+ GNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SATGKGE+II++TVARDVA
Sbjct: 181 DSQGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSATGKGEAIIQATVARDVA 240

Query: 259 ALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEI 315
           ALMEFKGLSL+EAA  VV E  PKG VGLIAVSA+GE+AMPFNTTGMFRACATEDG SEI
Sbjct: 241 ALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFNTTGMFRACATEDGNSEI 300

BLAST of CSPI01G26670 vs. TrEMBL
Match: A0A0A0LW19_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570100 PE=4 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 3.2e-174
Identity = 317/319 (99.37%), Postives = 317/319 (99.37%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180
           FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG
Sbjct: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMP  TTGMFR
Sbjct: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPLTTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 320
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

BLAST of CSPI01G26670 vs. TrEMBL
Match: M5WUV5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008761mg PE=4 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 9.5e-142
Identity = 261/317 (82.33%), Postives = 286/317 (90.22%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLP +RR PRE  LR CL IGV+ALK++ P LDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPPERRLPREAGLRHCLHIGVEALKAKTPALDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
            PHFNAG+GSVLTT+ TVEMEA IMD TKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  IPHFNAGKGSVLTTQGTVEMEACIMDGTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180
           FDGAEAFAREQGVET+DSS+FITP+N+ERL+QAKEANRVQIDYTQPL +  ++ET    G
Sbjct: 121 FDGAEAFAREQGVETVDSSHFITPENVERLKQAKEANRVQIDYTQPLHKDEKKETPEADG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS++GTVGCVAVD+ GNLASATSTGGLVNKMVGRIGDTP+IGAGTYAN LCA+SATGKGE
Sbjct: 181 DSKLGTVGCVAVDSLGNLASATSTGGLVNKMVGRIGDTPIIGAGTYANSLCAVSATGKGE 240

Query: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGMF 300
           +IIR TVARDVAA+MEFK +SL+EAAA VV E  PKGNVGL+AVSA+GEV MPFNT GMF
Sbjct: 241 AIIRGTVARDVAAVMEFKYVSLKEAAAYVVEEGTPKGNVGLVAVSATGEVTMPFNTNGMF 300

Query: 301 RACATEDGYSEISIWSS 317
           RACATEDGYSEI IW S
Sbjct: 301 RACATEDGYSEIGIWPS 317

BLAST of CSPI01G26670 vs. TrEMBL
Match: A0A061FX99_THECC (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein OS=Theobroma cacao GN=TCM_014361 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.0e-140
Identity = 256/317 (80.76%), Postives = 285/317 (89.91%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLP DRR PRE  LR CL +G+ ALK+ K PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPPDRRLPREAGLRHCLDVGIAALKAHKHPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDS-TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            PHFNAG+GSVLTT  TVEMEA IMD  TK+CGAVSGL+TVVN ISLAR VM+KTPHIYL
Sbjct: 61  HPHFNAGKGSVLTTSGTVEMEACIMDGKTKKCGAVSGLSTVVNAISLARKVMDKTPHIYL 120

Query: 121 AFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPS 180
           AFDGAEAFAREQG+ET+D SYFITP+NIERL+QA+EANRVQIDYTQP+ +  +++  +  
Sbjct: 121 AFDGAEAFAREQGLETVDPSYFITPENIERLKQAQEANRVQIDYTQPIQKEVKKDDASVD 180

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVDN GNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCA+SATGKG
Sbjct: 181 GDSQIGTVGCVAVDNEGNLASATSTGGLVNKMVGRIGDTPIIGAGTYANHLCAVSATGKG 240

Query: 241 ESIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMF 300
           E+IIR TVARDVAA+MEFKG+SL+EAAA VVE VP GNVGL+AVS++GEV MPFNTTGMF
Sbjct: 241 EAIIRGTVARDVAAVMEFKGVSLKEAAAYVVEQVPPGNVGLVAVSSTGEVTMPFNTTGMF 300

Query: 301 RACATEDGYSEISIWSS 317
           RACATEDG+SEI+IW S
Sbjct: 301 RACATEDGHSEIAIWDS 317

BLAST of CSPI01G26670 vs. TrEMBL
Match: B9RLJ0_RICCO (L-asparaginase, putative OS=Ricinus communis GN=RCOM_1467530 PE=4 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 3.4e-139
Identity = 259/321 (80.69%), Postives = 285/321 (88.79%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPL+LP +RR PRE  LR CL+IGV AL++QK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLNLPPERRLPREAALRHCLEIGVAALQAQKHPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDS-TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P+FNAG+GSVLTT+ TVEMEA IMD  TK+CGAVSGLTTVVN ISLARLVMEKTPHIYL
Sbjct: 61  HPNFNAGKGSVLTTEGTVEMEACIMDGKTKKCGAVSGLTTVVNAISLARLVMEKTPHIYL 120

Query: 121 AFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPS 180
           AFDGAEAFAREQGVET+DSS F+TP+N+ERL+QAKEANRVQIDYTQP+ +  E +  N  
Sbjct: 121 AFDGAEAFAREQGVETVDSSQFVTPENVERLKQAKEANRVQIDYTQPIQKNEETQNPNAD 180

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD +GNLASATSTGGLVNKMVGRIGDTP+IGAGTYAN+LCA+SATGKG
Sbjct: 181 GDSQIGTVGCVAVDTNGNLASATSTGGLVNKMVGRIGDTPIIGAGTYANNLCAVSATGKG 240

Query: 241 ESIIRSTVARDVAALMEFKGLSLEEAAASVVES-VPKGNVGLIAVSASGEVAMPFNTTGM 300
           E IIR TVARDVAALME+KGLSL EAAA VVE  VP+  VGL+AVSA+GEV MPFNTTGM
Sbjct: 241 EFIIRGTVARDVAALMEYKGLSLMEAAAYVVEQCVPRATVGLVAVSATGEVTMPFNTTGM 300

Query: 301 FRACATEDGYSEISIWSSKHD 320
           FRACATEDGYSEI+IW    D
Sbjct: 301 FRACATEDGYSEIAIWPPVQD 321

BLAST of CSPI01G26670 vs. TrEMBL
Match: D7SIN7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_17s0000g04910 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 4.4e-139
Identity = 256/316 (81.01%), Postives = 285/316 (90.19%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLP +RR+PRE  LR CLQIGV AL++++PPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPLERREPREAALRHCLQIGVAALQTKRPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P+FNAGRGSVLTT  TVEMEA IMD +TK+CGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 61  NPNFNAGRGSVLTTDGTVEMEACIMDGNTKKCGAVSGLTTVVNPISLARLVMEKTPHIYL 120

Query: 121 AFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPS 180
           AFDGAEAFAREQGVET+D+S+FITP+NIERL+QAKEANRVQIDYTQP+ + T++ET    
Sbjct: 121 AFDGAEAFAREQGVETVDASHFITPENIERLKQAKEANRVQIDYTQPITKDTKKETPTAD 180

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD  GNLA+ATSTGGLVNKMVGRIGDTP++GAG YAN LCA+SATGKG
Sbjct: 181 GDSQIGTVGCVAVDTRGNLAAATSTGGLVNKMVGRIGDTPIVGAGNYANSLCAVSATGKG 240

Query: 241 ESIIRSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 300
           E+II  TVARDVAALME+KGLSLEEAAA V+ E  P+G  GL+AVSA+GEV M FNTTGM
Sbjct: 241 EAIICGTVARDVAALMEYKGLSLEEAAAYVISECTPRGTAGLVAVSATGEVTMQFNTTGM 300

Query: 301 FRACATEDGYSEISIW 315
           FRACA+EDGYSEI IW
Sbjct: 301 FRACASEDGYSEIGIW 316

BLAST of CSPI01G26670 vs. TAIR10
Match: AT5G08100.1 (AT5G08100.1 N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein)

HSP 1 Score: 484.6 bits (1246), Expect = 4.8e-137
Identity = 251/316 (79.43%), Postives = 275/316 (87.03%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP+ LP +RR PRE  LR CL +G+ ALKS KPPLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDS-TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P FNAG+GSVLT + TVEMEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPS 180
           AFD AEAFAR  GVET+DSS+FITP+NI RL+QAKE NRVQ+DYT P P+  +       
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----C 181

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD+ GNLASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 ESIIRSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 300
           E IIR TVARDVAALME+KGLSL EAAA VV +SVP+G+ GL+AVSA+GEV MPFNTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEISIW 315
           FRACA+EDGYSEI+IW
Sbjct: 302 FRACASEDGYSEIAIW 312

BLAST of CSPI01G26670 vs. TAIR10
Match: AT3G16150.1 (AT3G16150.1 N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein)

HSP 1 Score: 355.5 bits (911), Expect = 3.4e-98
Identity = 189/323 (58.51%), Postives = 242/323 (74.92%), Query Frame = 1

Query: 2   GWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GWAIA+HGGAG  P +LP++R++  ++ L RCL +G+ AL+S    +DVVELV+RELE  
Sbjct: 3   GWAIAVHGGAGIDP-NLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETD 62

Query: 62  PHFNAGRGSVLTTKATVEMEASIMDSTKR-CGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
           P FN+GRGS LT K TVEMEASIMD TKR CGAVSG+TTV NPISLARLVM+K+PH YLA
Sbjct: 63  PLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYLA 122

Query: 122 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYT-QPLPQATEQETENPS 181
           F GAE FAR+QGVE +D+ YF+T  N+  L+ AKEAN +  DY   P+  A    T++P 
Sbjct: 123 FSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSPI 182

Query: 182 GDSQIG-------TVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA 241
             + +        TVGCV VD  G+ A+ TSTGGL+NKM+GRIGD+PLIGAGTYA+  C 
Sbjct: 183 QMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCG 242

Query: 242 ISATGKGESIIRSTVARDVAALMEFKGLSLEEAAASVVE-SVPKGNVGLIAVSASGEVAM 301
           +S TG+GE+IIR+T+ARDV+A+ME+KGL+L+EA   V++  + +G  GLIAVS  GEV  
Sbjct: 243 VSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVVC 302

Query: 302 PFNTTGMFRACATEDGYSEISIW 315
            FN+ GMFR CATEDG+ E++IW
Sbjct: 303 GFNSNGMFRGCATEDGFMEVAIW 324

BLAST of CSPI01G26670 vs. TAIR10
Match: AT4G00590.1 (AT4G00590.1 N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein)

HSP 1 Score: 85.5 bits (210), Expect = 6.5e-17
Identity = 87/335 (25.97%), Postives = 139/335 (41.49%), Query Frame = 1

Query: 5   IALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHF 64
           +A+H GAG       ++ +  R    R CL       +     +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTKATVEMEASIMDSTKRC-GAVSGLTTVVNPISLARLVME----------K 124
           NAGRGS LT    VE +AS+MD      G V  +  V N I +A L+++          +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 125 TPHIYLAFDGAEAFAREQ-----GVETIDSSYFITPQNIERLQQAK----EANRVQIDYT 184
            P + L  +GA  + + +     G  T    + +T +   + ++ K    E     I   
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 185 QPLPQATEQET---ENPSG------DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRI 244
           +  P+ TE      EN S       D  + TVG + VDN G++A  +S+GG+  K+ GR+
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRV 254

Query: 245 GDTPLIGAGTYAN--------HLCAISATGKGESIIRSTVARDVAALMEFKGLSLEEAAA 293
           G     G+G +A+         L     +G GE ++R   AR+    +         AA 
Sbjct: 255 GLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAM 314

BLAST of CSPI01G26670 vs. TAIR10
Match: AT5G61540.1 (AT5G61540.1 N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein)

HSP 1 Score: 65.1 bits (157), Expect = 9.1e-11
Identity = 67/258 (25.97%), Postives = 112/258 (43.41%), Query Frame = 1

Query: 51  VELVVRELENCPHFNA----GRGSVLTTKATVEMEASIMDS-TKRCGAVSGLTTVVNPIS 110
           VE VV     C         G G          ++A +MD  T   GAV+ +  V + I 
Sbjct: 60  VEAVVEGCSACEELRCDGTVGPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDGIR 119

Query: 111 LARLVMEKTPHIYLAFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQ 170
            A LVM+ + H  LA +GA AFA   G+         +P+++++    KE N+ Q ++ +
Sbjct: 120 AAHLVMKYSQHTLLAGEGASAFAISMGLP--GPMNLSSPESVKKWSDWKE-NQCQPNFRK 179

Query: 171 PLPQATEQETENPSGDS------------QIGTV----GCVAVDNH-----------GNL 230
            +  A +     P+  +            ++G +      V   NH           G++
Sbjct: 180 NVVPANDCGPYKPNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNHDTISMAVIDRMGHI 239

Query: 231 ASATSTGGLVNKMVGRIGDTPLIGAGTYA-NHLCAISATGKGESIIRSTVARDVAALMEF 276
           A  TST G   K+ GR+GD P++G+  YA + +    ATG G++++R      V   M  
Sbjct: 240 AVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMMRFLPCYQVVESMR- 299

BLAST of CSPI01G26670 vs. NCBI nr
Match: gi|449435681|ref|XP_004135623.1| (PREDICTED: isoaspartyl peptidase/L-asparaginase 1 [Cucumis sativus])

HSP 1 Score: 619.0 bits (1595), Expect = 4.6e-174
Identity = 317/319 (99.37%), Postives = 317/319 (99.37%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180
           FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG
Sbjct: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMP  TTGMFR
Sbjct: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPLTTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 320
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

BLAST of CSPI01G26670 vs. NCBI nr
Match: gi|659099549|ref|XP_008450657.1| (PREDICTED: isoaspartyl peptidase/L-asparaginase isoform X2 [Cucumis melo])

HSP 1 Score: 612.1 bits (1577), Expect = 5.7e-172
Identity = 312/319 (97.81%), Postives = 315/319 (98.75%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTK TVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180
           FDGAE FAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETEN +G
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300
           +IIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEISIWSSKHD 320
           ACATEDGYSEISIWSSKHD
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

BLAST of CSPI01G26670 vs. NCBI nr
Match: gi|659099547|ref|XP_008450655.1| (PREDICTED: isoaspartyl peptidase/L-asparaginase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 604.7 bits (1558), Expect = 9.0e-170
Identity = 312/327 (95.41%), Postives = 315/327 (96.33%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELV------ 60
           MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELV      
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVCREFVL 60

Query: 61  --VRELENCPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVME 120
             VRELENCPHFNAGRGSVLTTK TVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVME
Sbjct: 61  VQVRELENCPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVME 120

Query: 121 KTPHIYLAFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATE 180
           KTPHIYLAFDGAE FAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATE
Sbjct: 121 KTPHIYLAFDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATE 180

Query: 181 QETENPSGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA 240
           QETEN +GDS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCA
Sbjct: 181 QETENSNGDSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCA 240

Query: 241 ISATGKGESIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMP 300
           ISATGKGE+IIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMP
Sbjct: 241 ISATGKGEAIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMP 300

Query: 301 FNTTGMFRACATEDGYSEISIWSSKHD 320
           FNTTGMFRACATEDGYSEISIWSSKHD
Sbjct: 301 FNTTGMFRACATEDGYSEISIWSSKHD 327

BLAST of CSPI01G26670 vs. NCBI nr
Match: gi|672182772|ref|XP_008811641.1| (PREDICTED: isoaspartyl peptidase/L-asparaginase 1-like [Phoenix dactylifera])

HSP 1 Score: 514.2 bits (1323), Expect = 1.6e-142
Identity = 258/320 (80.62%), Postives = 288/320 (90.00%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGW+IALHGGAGDIP SLP +RR PRE  LR+CL IGV ALK+ +PPLDVVELVVRELEN
Sbjct: 1   MGWSIALHGGAGDIPYSLPPERRAPREAALRQCLAIGVAALKASRPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
           CPHFNAG+GSVLTT  TVEMEA IMD +TK+CGAVSGL+TVVN ISLARLVMEKTPHIYL
Sbjct: 61  CPHFNAGKGSVLTTDGTVEMEACIMDGNTKKCGAVSGLSTVVNAISLARLVMEKTPHIYL 120

Query: 121 AFDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPS 180
           AFDGAEAFAREQGVET+D+++FITP+N+ERL+QAKEANRVQIDYTQP+ +   QET +  
Sbjct: 121 AFDGAEAFAREQGVETVDANHFITPENVERLKQAKEANRVQIDYTQPVKEDKPQETSSGK 180

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD  GNLA+ATSTGGLVNKMVGRIGDTP+IGAGTYAN  CA+SATGKG
Sbjct: 181 GDSQIGTVGCVAVDGSGNLAAATSTGGLVNKMVGRIGDTPIIGAGTYANSFCAVSATGKG 240

Query: 241 ESIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMF 300
           ESIIR+T+ARDVAALME+KGLSL+EAAA VV +VP+GNVGL+AVSA GEV MPFNT GMF
Sbjct: 241 ESIIRATIARDVAALMEYKGLSLKEAAAYVVNNVPRGNVGLVAVSAKGEVTMPFNTRGMF 300

Query: 301 RACATEDGYSEISIWSSKHD 320
           RACATEDGYSEI IW S+ D
Sbjct: 301 RACATEDGYSEIGIWPSETD 320

BLAST of CSPI01G26670 vs. NCBI nr
Match: gi|645269605|ref|XP_008240074.1| (PREDICTED: isoaspartyl peptidase/L-asparaginase 1 [Prunus mume])

HSP 1 Score: 511.9 bits (1317), Expect = 8.0e-142
Identity = 262/317 (82.65%), Postives = 285/317 (89.91%), Query Frame = 1

Query: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIPLSLP +RR PRE  LR CL IGV+ALK+  P  DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPPERRLPREAGLRHCLHIGVEALKANTPASDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
            PHFNAG+GSVLTT+ TVEMEA IMD TKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  IPHFNAGKGSVLTTQGTVEMEACIMDGTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180
           FDGAEAFAREQGVET+DSS+FITP+N+ERLQQAKEANRVQIDYTQPL +  ++ET    G
Sbjct: 121 FDGAEAFAREQGVETVDSSHFITPENVERLQQAKEANRVQIDYTQPLHKDEKKETPEADG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS++GTVGCVAVD+ GNLASATSTGGLVNKMVGRIGDTP+IGAGTYAN LCA+SATGKGE
Sbjct: 181 DSKLGTVGCVAVDSLGNLASATSTGGLVNKMVGRIGDTPIIGAGTYANSLCAVSATGKGE 240

Query: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVV-ESVPKGNVGLIAVSASGEVAMPFNTTGMF 300
           +IIR TVARDVAA+MEFK +SL+EAAA VV E  PKGNVGL+AVSA+GEV MPFNTTGMF
Sbjct: 241 AIIRGTVARDVAAVMEFKCVSLKEAAAYVVEEGTPKGNVGLVAVSATGEVTMPFNTTGMF 300

Query: 301 RACATEDGYSEISIWSS 317
           RACATEDGYSEI IW S
Sbjct: 301 RACATEDGYSEIGIWPS 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ASPGA_ARATH8.5e-13679.43Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana GN=At5g08100 PE=1... [more]
ASPG_LUPAN2.5e-13579.38Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius PE=2 SV=1[more]
ASPG_LUPLU2.5e-13578.75Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus PE=1 SV=1[more]
ASPG_LUPAL1.0e-13377.19Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus PE=3 SV=1[more]
ASPG_LUPAR4.1e-12276.90Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LW19_CUCSA3.2e-17499.37Uncharacterized protein OS=Cucumis sativus GN=Csa_1G570100 PE=4 SV=1[more]
M5WUV5_PRUPE9.5e-14282.33Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008761mg PE=4 SV=1[more]
A0A061FX99_THECC1.0e-14080.76N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein OS=T... [more]
B9RLJ0_RICCO3.4e-13980.69L-asparaginase, putative OS=Ricinus communis GN=RCOM_1467530 PE=4 SV=1[more]
D7SIN7_VITVI4.4e-13981.01Putative uncharacterized protein OS=Vitis vinifera GN=VIT_17s0000g04910 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G08100.14.8e-13779.43 N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily ... [more]
AT3G16150.13.4e-9858.51 N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily ... [more]
AT4G00590.16.5e-1725.97 N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily ... [more]
AT5G61540.19.1e-1125.97 N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily ... [more]
Match NameE-valueIdentityDescription
gi|449435681|ref|XP_004135623.1|4.6e-17499.37PREDICTED: isoaspartyl peptidase/L-asparaginase 1 [Cucumis sativus][more]
gi|659099549|ref|XP_008450657.1|5.7e-17297.81PREDICTED: isoaspartyl peptidase/L-asparaginase isoform X2 [Cucumis melo][more]
gi|659099547|ref|XP_008450655.1|9.0e-17095.41PREDICTED: isoaspartyl peptidase/L-asparaginase 1 isoform X1 [Cucumis melo][more]
gi|672182772|ref|XP_008811641.1|1.6e-14280.63PREDICTED: isoaspartyl peptidase/L-asparaginase 1-like [Phoenix dactylifera][more]
gi|645269605|ref|XP_008240074.1|8.0e-14282.65PREDICTED: isoaspartyl peptidase/L-asparaginase 1 [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000246Peptidase_T2
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006522 alanine metabolic process
biological_process GO:0006528 asparagine metabolic process
biological_process GO:0006531 aspartate metabolic process
biological_process GO:0006508 proteolysis
biological_process GO:0008150 biological_process
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0004067 asparaginase activity
molecular_function GO:0008798 beta-aspartyl-peptidase activity
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G26670.1CSPI01G26670.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 1..319
score: 1.2E
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 3..314
score: 1.5E
NoneNo IPR availablePANTHERPTHR10188:SF24ISOASPARTYL PEPTIDASE/L-ASPARAGINASE 1coord: 1..319
score: 1.2E