Bhi02G001689 (gene) Wax gourd

NameBhi02G001689
Typegene
OrganismBenincasa hispida (Wax gourd)
Descriptionisoaspartyl peptidase/L-asparaginase
Locationchr2 : 55346874 .. 55350550 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGAGACAAACTTCATATTTCGATCGAGCCAAACAGAAATGACAGTAAAACGTTGAATAGCAGTACACTATAAAATTTCCTGACCGAAGCAGAATGGAGAAGTTGCACCAACTCCGCTACTAAATTCACAATCCTTCTCCATTAATACACTCTCGCCAATCGCCGGCGAATGACCTGGATAATCCTTTAGCGATGGGATGGGCAATAGCTCTTCACGGCGGCGCCGGCGACATCCCCCACTCATTGCCACCTGAACGCCGCCAACCGCGCGAGGAAACCCTCCGCCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAATCCCAGAAGCCTCCTTTGGATGTAGTTGAACTCGTGGTGAGAAATTTTTACCTTTTTTTTTTTTTTTTTCTTTCACTCTGTCTTGATATGACTTTTAGTTCCATGAGTTTTAGAATCTAATGATTTAGTTTAGTGTGTGTAATCTTCAAGTTTGTACTGAATTTGTTTACTGGTTTTAGTATGTAATGATATAGTTCAAGATGCAGTTTAAATTTATAATGATTTAGTCTCTAATGTGAGAAAATTTGATTAGGTGTAATGGAGTTTCGTAATTAGATTGATGGATGAATTGCTTAGAGACCTAATAAAGTTTTCATGGATTTTAATCCGGGGATTAAATTCTTATCTTCCTGAGGATTAAATCAATATAAATATTAAATCACAATGACTTTCTTTTCTGTCTCCCTTTCTTTCTACTTGCTGAAACGCAGCTAATCTGGGTCCTTGCTTTTCTGCTAATGGGAGGTTGCGTGTGCTTTCATTTGGTTTATTAAGTACTTTCTTGTGATGATTCCCTTACTGATAAGTTAATGGCTTTGGGGATTTGAGTTTTGATGTTATATACAAATTATGCCCTTTTAAATTTTAAGGCTAAATGCACTTTTGGTCCCTGAGGTTTAGAGTAAGTGTCTATTTGGTCCTTAAGATTTCAAAACATACATTTTAGTCCTTGGGGTTTGAAAAATGGTCCTTGAGTTAATTTGACCGTTAGTTAACTAATGTAAATTTGACGTGCCAAGTTGAGATGACATATTTTAAATAAGGTGGATAACTTTCCTAAGTTTATATTATTTTTAGGTCACATGGATTTTTTAAGTATTTTTTTCCCTCTCCGCCCTAAAAATTTTGACAAAAATATATTTTCAGCTCATTCGTTGATGGCTGTTAAGCATGGAAATGGATCATTGATGGAAATTGGAGAACTTATTGTTAGCAATTTCTTAGGGGGTGTTTGTCCTATGGATTAATCAGTCATCAGTTGTGATAGTTACAAAAATTTGAATTGTGTTAGTTCTGGGAATATATTATTTGTAAAAGACAATATAGACTTAGGTGAGTTTCATAAGTTTATATTTGATGGTCTAGAAAGATCATATGAACTAAGGATAATATCTTTTATTTTAGTGACCTTTAGCTTTAGTTTACATAACTTGAAATTTATGTTATATGTGGGATCAGCAGTCTCTATAGTTGTAAATAAGTAAACCTATATGTACTAACATAAGTTTTAATCAACTCTCTAGATTACACAAATTTAATTTGTGAGTCAAACATGTCCTGATAATTTAAAACTCATTTGATGGTTTAGATTATCTAAACTTAGGGTGAACATCTTCTGTTCTAATCACTTTTACTTTCAAGTCTGACCCTTTTGTTTGAACATTTTGTTTGGTAACCCGAATTCATAGTTGTTGCCCCTAATCAAGATCTAATAAGTTTCATTGTAGTATAAATCATTTTGACTAGATAATCAGTTCTTTCTTATCCATGGCTTCTTCTCCTTGTTAAGATGATTAGTTTTTTTTTTTTTTTTNNNNTTTTTTTTTTTTTTTTTTTTTTTTTCTTGTACAGGTACGAGAACTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGATCTGTCTTAACAACCAAGGGAACCATCGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCTGGGCTCACCACTGTTGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGTAAGCAATTTCTATCCCTCTTCCTATGTAGCTGCAGAATCAATCCTCTCTTTACATATTATTTTGGCACTAATACAGATAGATTCATTGTGATTCATGACTCTTTTATTTCTCAAAAAATGTCGAGGGAGTGGAGACCACTAATTCAAGCCACTTTATCACACCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGAGTGCAGGTTTTCTGCTTACCTTTTATCCCAATACATGAATTAACTGCTCTCTTTGTCTCTGAACTTACCTGCATATTATATTGTTAAGCTAGAATTATGTCAACGTCTAGGCATTTTCACAATATGTTAAATATTGATGTTGATAAAAATGTCGAGATTTTAATATTATAAAAATTAAGAGGAAATATCAATAAAATGTCAGTGTTAGATGGATATTTCTAAGGAATTTATAAAAGAAAATGTGCGAGTTAGAGAAATTGGTAATCCACGGTTTTTAAAATAAGTTAAAAGTTCTTAATGTCGTTTAATCTTGTTTAAATTTTTAATATTTTGTTGGTGCGTAAGGTATAACAGAAATATTGATTCACCATATACGTCGATGTTGAACCTATAAATATATGACAATGTTGATGAAAATATTGATATGTTGACGAAAATTTCATGCCTCGGTTGCTAAAACTCTTCATAGATCAGAAGTTTCTGATTATATATATAGGTTATAGATCTAGCTGTGCATCCAATAAGGTTGAAAAAGACGCATTACGGAGTGTTTTTTTTATAGCATCTGGCGTTTTTTATTTTTTATGGTACTTTCTTGTCTGCACAGTGATAGCCCTCTGTTTTGAACCCTTGTAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAACGCAAATGGTGATAGTCAAATAGGGACTGTTGGATGTGTAGCTGTTGATAACCACGGGAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGCGACACTCCATTAATAGGGGCTGGGACATATGCTAACCATCTTTGTGCAATTTCTGCAACTGGAAAGGGCGAAGCCATCATACGCGGTACCGTGGCGAGGGATGTTGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTTGAAGAAGCAACAGCCTACGTTGTTGAAAGTGTTCCCAAAGGCAATGTCGGTTTGGTTGCTGTATCCGCCTCAGGTGAAGTTGCCATACCTTACAATACGACCGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTACTCTGAGATTGCCATATGGTCTTCTAAAAATGACTGAATGAATATTGTGACAAATCCAGAGCTGAAATTTGGTTCTATTCTGATGTTTTGATGGTTGGTGTCTCTCAGCTTTTGTTGTTTTTCCTAAATATTAAGAATAGTCTTCCAAATGTTCTTCCATTTCATGTATTTGGTTCAATTTCAATGGCAAGATATTCTCAATTATTAGTTAAACATAAGTACAAGATATTTTCTCCTCCCTACATATATACTTAAAATTTTCTACCCA

mRNA sequence

ATTGAGACAAACTTCATATTTCGATCGAGCCAAACAGAAATGACAGTAAAACGTTGAATAGCAGTACACTATAAAATTTCCTGACCGAAGCAGAATGGAGAAGTTGCACCAACTCCGCTACTAAATTCACAATCCTTCTCCATTAATACACTCTCGCCAATCGCCGGCGAATGACCTGGATAATCCTTTAGCGATGGGATGGGCAATAGCTCTTCACGGCGGCGCCGGCGACATCCCCCACTCATTGCCACCTGAACGCCGCCAACCGCGCGAGGAAACCCTCCGCCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAATCCCAGAAGCCTCCTTTGGATGTAGTTGAACTCGTGGTACGAGAACTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGATCTGTCTTAACAACCAAGGGAACCATCGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCTGGGCTCACCACTGTTGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGGAGTGGAGACCACTAATTCAAGCCACTTTATCACACCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGAGTGCAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAACGCAAATGGTGATAGTCAAATAGGGACTGTTGGATGTGTAGCTGTTGATAACCACGGGAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGCGACACTCCATTAATAGGGGCTGGGACATATGCTAACCATCTTTGTGCAATTTCTGCAACTGGAAAGGGCGAAGCCATCATACGCGGTACCGTGGCGAGGGATGTTGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTTGAAGAAGCAACAGCCTACGTTGTTGAAAGTGTTCCCAAAGGCAATGTCGGTTTGGTTGCTGTATCCGCCTCAGGTGAAGTTGCCATACCTTACAATACGACCGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTACTCTGAGATTGCCATATGGTCTTCTAAAAATGACTGAATGAATATTGTGACAAATCCAGAGCTGAAATTTGGTTCTATTCTGATGTTTTGATGGTTGGTGTCTCTCAGCTTTTGTTGTTTTTCCTAAATATTAAGAATAGTCTTCCAAATGTTCTTCCATTTCATGTATTTGGTTCAATTTCAATGGCAAGATATTCTCAATTATTAGTTAAACATAAGTACAAGATATTTTCTCCTCCCTACATATATACTTAAAATTTTCTACCCA

Coding sequence (CDS)

ATGGGATGGGCAATAGCTCTTCACGGCGGCGCCGGCGACATCCCCCACTCATTGCCACCTGAACGCCGCCAACCGCGCGAGGAAACCCTCCGCCGTTGCCTGCAGATCGGCGTTCAAGCTCTCAAATCCCAGAAGCCTCCTTTGGATGTAGTTGAACTCGTGGTACGAGAACTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGATCTGTCTTAACAACCAAGGGAACCATCGAAATGGAAGCTAGCATCATGGACAGTACGAAGAGATGTGGAGCTGTTTCTGGGCTCACCACTGTTGTTAATCCCATATCTTTAGCTAGACTTGTTATGGAGAAAACTCCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGGAGTGGAGACCACTAATTCAAGCCACTTTATCACACCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGAGTGCAGATTGATTATACGCAACCACTTCCACAAGCCACAGAACAAGAAACTGAGAACGCAAATGGTGATAGTCAAATAGGGACTGTTGGATGTGTAGCTGTTGATAACCACGGGAATTTAGCTTCTGCAACTTCCACTGGTGGATTAGTGAACAAAATGGTTGGTAGGATTGGCGACACTCCATTAATAGGGGCTGGGACATATGCTAACCATCTTTGTGCAATTTCTGCAACTGGAAAGGGCGAAGCCATCATACGCGGTACCGTGGCGAGGGATGTTGCAGCTCTAATGGAGTTCAAGGGCCTGTCTCTTGAAGAAGCAACAGCCTACGTTGTTGAAAGTGTTCCCAAAGGCAATGTCGGTTTGGTTGCTGTATCCGCCTCAGGTGAAGTTGCCATACCTTACAATACGACCGGAATGTTTCGAGCTTGTGCAACTGAAGATGGTTACTCTGAGATTGCCATATGGTCTTCTAAAAATGACTGA

Protein sequence

MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFRACATEDGYSEIAIWSSKND
BLAST of Bhi02G001689 vs. Swiss-Prot
Match: sp|P30364|ASPG_LUPAN (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 1.9e-138
Identity = 256/320 (80.00%), Postives = 280/320 (87.50%), Query Frame = 0

Query: 2   GWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLPPERRQPREE LR CLQIGV+ALKSQKPPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GT+EMEASIMD  T +CGAVSGL+TV+NPISLARLVM+KTPHIYLA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYT-----QPLPQATEQET 181
           F GA+ FA++QGVET +SSHFIT +N+ERLK A EANRVQ+DY+     QP     E+E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEKEL 182

Query: 182 ENANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
             ANGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYN 301
           TGKGEAII  TVARDVAALMEFKGLSL+EA  YVV E  PKG VGL+AVSA+GE+A+P+N
Sbjct: 243 TGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIW 315
           TTGMFRA ATEDGYSEIAIW
Sbjct: 303 TTGMFRASATEDGYSEIAIW 322

BLAST of Bhi02G001689 vs. Swiss-Prot
Match: sp|Q9ZSD6|ASPG_LUPLU (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 1.6e-137
Identity = 251/320 (78.44%), Postives = 281/320 (87.81%), Query Frame = 0

Query: 2   GWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLPPERR+PREE LR CLQIGV+ALK+QKPPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GT+EMEASIMD +T +CGAVSGL+TV+NPISLARLVM+KTPHIYLA
Sbjct: 63  EHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQ-----PLPQATEQET 181
           F GA+ FA++QGVET +SSH IT +N+ERLK A EANRVQ+DY+Q     P+    E+E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEKEL 182

Query: 182 ENANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
              NGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLTNGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYN 301
           TGKGE IIR TVARDVAALMEFKGLSL+EA  +V+ E  PKG VGL+AVSA+GE+A+P+N
Sbjct: 243 TGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIW 315
           TTGMFRACATEDGYSEIAIW
Sbjct: 303 TTGMFRACATEDGYSEIAIW 322

BLAST of Bhi02G001689 vs. Swiss-Prot
Match: sp|P50287|ASPGA_ARATH (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 488.4 bits (1256), Expect = 6.0e-137
Identity = 254/316 (80.38%), Postives = 275/316 (87.03%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP  LP ERR PRE  LR CL +G+ ALKS KPPLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P FNAG+GSVLT +GT+EMEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENAN 180
           AFD AEAFAR  GVET +SSHFITP+NI RLKQAKE NRVQ+DYT P P+  +       
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----C 181

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD+ GNLASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 EAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYNTTGM 300
           E IIRGTVARDVAALME+KGLSL EA AYVV +SVP+G+ GLVAVSA+GEV +P+NTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEIAIW 315
           FRACA+EDGYSEIAIW
Sbjct: 302 FRACASEDGYSEIAIW 312

BLAST of Bhi02G001689 vs. Swiss-Prot
Match: sp|P50288|ASPG_LUPAL (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 3.9e-136
Identity = 250/320 (78.12%), Postives = 278/320 (86.88%), Query Frame = 0

Query: 2   GWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SLPPERR+PREE LR CLQIG++ALK+Q PPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
            HFNAG GSVLT  GT+EMEASIMD +T +CGAVSGL TV+NPISLAR VM+KTPHI+LA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIFLA 122

Query: 122 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQID-----YTQPLPQATEQET 181
           F GA+   ++QGVET +SSHFIT +N+ERLK A EANRVQ+D     YTQP+    E+E 
Sbjct: 123 FQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEKEL 182

Query: 182 ENANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISA 241
             ANGDSQIGTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYN 301
           TGKGEAIIR TVARDVAALMEFKGLSL+EA   VV E  PKG VGL+AVSA+GE+A+P+N
Sbjct: 243 TGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIW 315
           TTGMFRACATEDGYSEIAIW
Sbjct: 303 TTGMFRACATEDGYSEIAIW 322

BLAST of Bhi02G001689 vs. Swiss-Prot
Match: sp|P30362|ASPG_LUPAR (Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.1e-125
Identity = 237/303 (78.22%), Postives = 264/303 (87.13%), Query Frame = 0

Query: 19  PPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHFNAGRGSVLTTKGTI 78
           PPERR+PREE LR CLQIGV+ALK++K PLDVVELVVRELEN  HFNAG GSVLT  GT+
Sbjct: 1   PPERRKPREEGLRHCLQIGVEALKARKSPLDVVELVVRELENNEHFNAGIGSVLTNSGTV 60

Query: 79  EMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDGAEAFAREQGVETTN 138
           EMEASIMD  + +CGAVSGL+TV+NPISLARLVMEKTPH+YLAF GA+ FA++QGVET +
Sbjct: 61  EMEASIMDGKSMKCGAVSGLSTVLNPISLARLVMEKTPHMYLAFQGAQDFAKQQGVETVD 120

Query: 139 SSHFITPQNIERLKQAKEANRVQID-----YTQPLPQATEQETENANGDSQIGTVGCVAV 198
           SSHFIT +N+ERLK A EANRVQID     YTQP+    E+E   ANGDSQIGTVGCVAV
Sbjct: 121 SSHFITAENVERLKLAIEANRVQIDYSQYNYTQPVQDDAEKELPVANGDSQIGTVGCVAV 180

Query: 199 DNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGEAIIRGTVARDVA 258
           D+ GNLASATSTGGLVNKMVGRIGDTPLIGAGTYAN LCA+SATGKGEAII+ TVARDVA
Sbjct: 181 DSQGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSATGKGEAIIQATVARDVA 240

Query: 259 ALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYNTTGMFRACATEDGYSEI 315
           ALMEFKGLSL+EA  YVV E  PKG VGL+AVSA+GE+A+P+NTTGMFRACATEDG SEI
Sbjct: 241 ALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFNTTGMFRACATEDGNSEI 300

BLAST of Bhi02G001689 vs. TAIR10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein)

HSP 1 Score: 488.4 bits (1256), Expect = 3.3e-138
Identity = 254/316 (80.38%), Postives = 275/316 (87.03%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP  LP ERR PRE  LR CL +G+ ALKS KPPLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            P FNAG+GSVLT +GT+EMEASIMD  TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENAN 180
           AFD AEAFAR  GVET +SSHFITP+NI RLKQAKE NRVQ+DYT P P+  +       
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVPDN-----C 181

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD+ GNLASATSTGG VNKMVGRIGDTP+IGAGTYANHLCAISATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 EAIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYNTTGM 300
           E IIRGTVARDVAALME+KGLSL EA AYVV +SVP+G+ GLVAVSA+GEV +P+NTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEIAIW 315
           FRACA+EDGYSEIAIW
Sbjct: 302 FRACASEDGYSEIAIW 312

BLAST of Bhi02G001689 vs. TAIR10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein)

HSP 1 Score: 355.9 bits (912), Expect = 2.6e-98
Identity = 190/323 (58.82%), Postives = 238/323 (73.68%), Query Frame = 0

Query: 2   GWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENC 61
           GWAIA+HGGAG I  +LP ER++  ++ L RCL +G+ AL+S    +DVVELV+RELE  
Sbjct: 3   GWAIAVHGGAG-IDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETD 62

Query: 62  PHFNAGRGSVLTTKGTIEMEASIMDSTK-RCGAVSGLTTVVNPISLARLVMEKTPHIYLA 121
           P FN+GRGS LT KGT+EMEASIMD TK RCGAVSG+TTV NPISLARLVM+K+PH YLA
Sbjct: 63  PLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYLA 122

Query: 122 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPL-----PQATEQET 181
           F GAE FAR+QGVE  ++ +F+T  N+  LK AKEAN +  DY  P        AT+   
Sbjct: 123 FSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSPI 182

Query: 182 ENANGDSQI---GTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA 241
           +       I    TVGCV VD  G+ A+ TSTGGL+NKM+GRIGD+PLIGAGTYA+  C 
Sbjct: 183 QMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCG 242

Query: 242 ISATGKGEAIIRGTVARDVAALMEFKGLSLEEATAYVVE-SVPKGNVGLVAVSASGEVAI 301
           +S TG+GEAIIR T+ARDV+A+ME+KGL+L+EA  YV++  + +G  GL+AVS  GEV  
Sbjct: 243 VSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVVC 302

Query: 302 PYNTTGMFRACATEDGYSEIAIW 315
            +N+ GMFR CATEDG+ E+AIW
Sbjct: 303 GFNSNGMFRGCATEDGFMEVAIW 324

BLAST of Bhi02G001689 vs. TAIR10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein)

HSP 1 Score: 89.0 bits (219), Expect = 5.9e-18
Identity = 87/335 (25.97%), Postives = 140/335 (41.79%), Query Frame = 0

Query: 5   IALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELENCPHF 64
           +A+H GAG   +      +  R    R CL       +     +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVME----------K 124
           NAGRGS LT  G +E +AS+MD  +   G V  +  V N I +A L+++          +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 125 TPHIYLAFDGAEAFAREQ-----GVETTNSSHFITPQNIERLKQAK----EANRVQIDYT 184
            P + L  +GA  + + +     G  T      +T +   + ++ K    E     I   
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 185 QPLPQATEQE---TEN------ANGDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRI 244
           +  P+ TE      EN      A+ D  + TVG + VDN G++A  +S+GG+  K+ GR+
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRV 254

Query: 245 GDTPLIGAGTYAN--------HLCAISATGKGEAIIRGTVARDVAALMEFKGLSLEEATA 293
           G     G+G +A+         L     +G GE ++RG  AR+    +         A  
Sbjct: 255 GLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAM 314

BLAST of Bhi02G001689 vs. TAIR10
Match: AT5G61540.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein)

HSP 1 Score: 70.1 bits (170), Expect = 2.8e-12
Identity = 73/277 (26.35%), Postives = 118/277 (42.60%), Query Frame = 0

Query: 51  VELVVRELENCPHF----NAGRGSVLTTKGTIEMEASIMDS-TKRCGAVSGLTTVVNPIS 110
           VE VV     C         G G      G   ++A +MD  T   GAV+ +  V + I 
Sbjct: 60  VEAVVEGCSACEELRCDGTVGPGGSPDENGETMIDALVMDGVTMEVGAVAAMRYVKDGIR 119

Query: 111 LARLVMEKTPHIYLAFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQ 170
            A LVM+ + H  LA +GA AFA   G+      +  +P+++++    KE N+ Q ++ +
Sbjct: 120 AAHLVMKYSQHTLLAGEGASAFAISMGL--PGPMNLSSPESVKKWSDWKE-NQCQPNFRK 179

Query: 171 PLPQA----------------TEQETENAN-----------GDSQIGTVGCVAVDNHGNL 230
            +  A                 ++ TE+             G     T+    +D  G++
Sbjct: 180 NVVPANDCGPYKPNNSAMNVFVDKSTESCEMGAIEYKPPLVGPHNHDTISMAVIDRMGHI 239

Query: 231 ASATSTGGLVNKMVGRIGDTPLIGAGTYA-NHLCAISATGKGEAIIRGTVARDVAALMEF 290
           A  TST G   K+ GR+GD P++G+  YA + +    ATG G+ ++R      V   M  
Sbjct: 240 AVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMMRFLPCYQVVESMR- 299

BLAST of Bhi02G001689 vs. TrEMBL
Match: tr|A0A1S3BPR4|A0A1S3BPR4_CUCME (isoaspartyl peptidase/L-asparaginase OS=Cucumis melo OX=3656 GN=LOC103492179 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 5.9e-166
Identity = 299/319 (93.73%), Postives = 311/319 (97.49%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGT+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAE FAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN+NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

BLAST of Bhi02G001689 vs. TrEMBL
Match: tr|A0A0A0LW19|A0A0A0LW19_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570100 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 2.5e-164
Identity = 298/319 (93.42%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTK T+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN +G
Sbjct: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           +IIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P  TTGMFR
Sbjct: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPLTTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

BLAST of Bhi02G001689 vs. TrEMBL
Match: tr|A0A2H3ZD04|A0A2H3ZD04_PHODC (isoaspartyl peptidase/L-asparaginase 1-like OS=Phoenix dactylifera OX=42345 GN=LOC103722747 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 7.6e-145
Identity = 259/320 (80.94%), Postives = 289/320 (90.31%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGW+IALHGGAGDIP+SLPPERR PRE  LR+CL IGV ALK+ +PPLDVVELVVRELEN
Sbjct: 1   MGWSIALHGGAGDIPYSLPPERRAPREAALRQCLAIGVAALKASRPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
           CPHFNAG+GSVLTT GT+EMEA IMD +TK+CGAVSGL+TVVN ISLARLVMEKTPHIYL
Sbjct: 61  CPHFNAGKGSVLTTDGTVEMEACIMDGNTKKCGAVSGLSTVVNAISLARLVMEKTPHIYL 120

Query: 121 AFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENAN 180
           AFDGAEAFAREQGVET +++HFITP+N+ERLKQAKEANRVQIDYTQP+ +   QET +  
Sbjct: 121 AFDGAEAFAREQGVETVDANHFITPENVERLKQAKEANRVQIDYTQPVKEDKPQETSSGK 180

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQIGTVGCVAVD  GNLA+ATSTGGLVNKMVGRIGDTP+IGAGTYAN  CA+SATGKG
Sbjct: 181 GDSQIGTVGCVAVDGSGNLAAATSTGGLVNKMVGRIGDTPIIGAGTYANSFCAVSATGKG 240

Query: 241 EAIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMF 300
           E+IIR T+ARDVAALME+KGLSL+EA AYVV +VP+GNVGLVAVSA GEV +P+NT GMF
Sbjct: 241 ESIIRATIARDVAALMEYKGLSLKEAAAYVVNNVPRGNVGLVAVSAKGEVTMPFNTRGMF 300

Query: 301 RACATEDGYSEIAIWSSKND 320
           RACATEDGYSEI IW S+ D
Sbjct: 301 RACATEDGYSEIGIWPSETD 320

BLAST of Bhi02G001689 vs. TrEMBL
Match: tr|M5WUV5|M5WUV5_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G209700 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.3e-144
Identity = 265/317 (83.60%), Postives = 289/317 (91.17%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLPPERR PRE  LR CL IGV+ALK++ P LDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPPERRLPREAGLRHCLHIGVEALKAKTPALDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
            PHFNAG+GSVLTT+GT+EMEA IMD TKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  IPHFNAGKGSVLTTQGTVEMEACIMDGTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVET +SSHFITP+N+ERLKQAKEANRVQIDYTQPL +  ++ET  A+G
Sbjct: 121 FDGAEAFAREQGVETVDSSHFITPENVERLKQAKEANRVQIDYTQPLHKDEKKETPEADG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS++GTVGCVAVD+ GNLASATSTGGLVNKMVGRIGDTP+IGAGTYAN LCA+SATGKGE
Sbjct: 181 DSKLGTVGCVAVDSLGNLASATSTGGLVNKMVGRIGDTPIIGAGTYANSLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVV-ESVPKGNVGLVAVSASGEVAIPYNTTGMF 300
           AIIRGTVARDVAA+MEFK +SL+EA AYVV E  PKGNVGLVAVSA+GEV +P+NT GMF
Sbjct: 241 AIIRGTVARDVAAVMEFKYVSLKEAAAYVVEEGTPKGNVGLVAVSATGEVTMPFNTNGMF 300

Query: 301 RACATEDGYSEIAIWSS 317
           RACATEDGYSEI IW S
Sbjct: 301 RACATEDGYSEIGIWPS 317

BLAST of Bhi02G001689 vs. TrEMBL
Match: tr|A0A2P4LGJ9|A0A2P4LGJ9_QUESU (Isoaspartyl peptidase/l-asparaginase OS=Quercus suber OX=58331 GN=CFP56_64533 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 2.2e-144
Identity = 267/318 (83.96%), Postives = 291/318 (91.51%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SL  ERR+PRE  LR CL++GV ALK+QK PLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPVSLAAERRKPREAALRHCLKVGVNALKAQKSPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMD-STKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120
            PH+NAGRGSVLT +GTIEMEASIMD +TKRCGAVSGLTTVVNPISLARLVMEKTPHIYL
Sbjct: 61  NPHYNAGRGSVLTREGTIEMEASIMDGNTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 120

Query: 121 AFDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENAN 180
           AFDGAE FAREQG+ET +SSHFIT +NIERLKQAKEANRVQIDYT P+ +  ++ET  AN
Sbjct: 121 AFDGAERFAREQGIETVDSSHFITQENIERLKQAKEANRVQIDYTPPIQKDEKKETPTAN 180

Query: 181 GDSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKG 240
           GDSQ+GTVGCVAVD+HGNLASATSTGGLVNKMVGRIGDTP+IGAGTYAN+LCA+SATGKG
Sbjct: 181 GDSQLGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPIIGAGTYANNLCAVSATGKG 240

Query: 241 EAIIRGTVARDVAALMEFKGLSLEEATAYVVES-VPKGNVGLVAVSASGEVAIPYNTTGM 300
           EAI+RGTVARDVAALMEFKGLSL+EA AYVVE  VP+GNVGLVAVSA GEV + YNTTGM
Sbjct: 241 EAIMRGTVARDVAALMEFKGLSLKEAAAYVVEECVPRGNVGLVAVSAKGEVTMAYNTTGM 300

Query: 301 FRACATEDGYSEIAIWSS 317
           FRACATEDG+SEIAIW S
Sbjct: 301 FRACATEDGHSEIAIWPS 318

BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_008450657.1 (PREDICTED: isoaspartyl peptidase/L-asparaginase [Cucumis melo])

HSP 1 Score: 592.4 bits (1526), Expect = 9.0e-166
Identity = 299/319 (93.73%), Postives = 311/319 (97.49%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTKGT+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKGTVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAE FAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN+NG
Sbjct: 121 FDGAETFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENSNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DS IGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTP+IGAGTYANHLCAISATGKGE
Sbjct: 181 DSHIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPVIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_004135623.1 (PREDICTED: isoaspartyl peptidase/L-asparaginase 1 [Cucumis sativus] >KGN66085.1 hypothetical protein Csa_1G570100 [Cucumis sativus])

HSP 1 Score: 587.0 bits (1512), Expect = 3.8e-164
Identity = 298/319 (93.42%), Postives = 309/319 (96.87%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SLP +RRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLPSDRRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTTK T+EMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTKATVEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVET +SS+FITPQNIERL+QAKEANRVQIDYTQPLPQATEQETEN +G
Sbjct: 121 FDGAEAFAREQGVETIDSSYFITPQNIERLQQAKEANRVQIDYTQPLPQATEQETENPSG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           +IIR TVARDVAALMEFKGLSLEEA A VVESVPKGNVGL+AVSASGEVA+P  TTGMFR
Sbjct: 241 SIIRSTVARDVAALMEFKGLSLEEAAASVVESVPKGNVGLIAVSASGEVAMPLTTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEI+IWSSK+D
Sbjct: 301 ACATEDGYSEISIWSSKHD 319

BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_023530268.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 569.7 bits (1467), Expect = 6.2e-159
Identity = 289/316 (91.46%), Postives = 299/316 (94.62%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SL  +RRQPREETLRRCLQIGVQALK QKPP+DVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPVDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GT+EMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVETT+ S+FITPQNIERLKQAKEAN VQIDYTQP PQAT QETE  NG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATGQETEIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA+SATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIRGTVARDVAALMEFKGLSLEEA AY VESVPKGNVGL+AVSASGEVA+P+NTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKGLSLEEAAAYAVESVPKGNVGLIAVSASGEVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSS 317
           ACATEDGYSEIAIW S
Sbjct: 301 ACATEDGYSEIAIWPS 316

BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_022933679.1 (isoaspartyl peptidase/L-asparaginase 1 [Cucurbita moschata])

HSP 1 Score: 563.5 bits (1451), Expect = 4.5e-157
Identity = 287/316 (90.82%), Postives = 297/316 (93.99%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP SL  +RRQPREETLRRCLQIGVQALK QKPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLQIGVQALKEQKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
           CPHFNAGRGSVLTT GT+EMEASIMD  KRCGAVSGLTTVVN ISLARLVMEKTPHIYLA
Sbjct: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVETT+ S+FITPQNIERLKQAKEAN VQIDYTQP PQAT QETE  NG
Sbjct: 121 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETEIGNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQIGTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA+SATGKGE
Sbjct: 181 DSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIRGTVARDVAALMEFK LSLEEA A  VESVPKGNVGL+AVSASG+VA+P+NTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKDLSLEEAAACAVESVPKGNVGLIAVSASGDVAMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSS 317
           ACATEDGYSEIAIW S
Sbjct: 301 ACATEDGYSEIAIWPS 316

BLAST of Bhi02G001689 vs. NCBI nr
Match: XP_022156717.1 (isoaspartyl peptidase/L-asparaginase 1 [Momordica charantia])

HSP 1 Score: 562.0 bits (1447), Expect = 1.3e-156
Identity = 280/319 (87.77%), Postives = 301/319 (94.36%), Query Frame = 0

Query: 1   MGWAIALHGGAGDIPHSLPPERRQPREETLRRCLQIGVQALKSQKPPLDVVELVVRELEN 60
           MGWAIALHGGAGDIP +LPP+RR PREE LR CL +GV+ALK+ KPPLDVVELVVRELEN
Sbjct: 1   MGWAIALHGGAGDIPLTLPPDRRHPREEALRHCLSVGVEALKAHKPPLDVVELVVRELEN 60

Query: 61  CPHFNAGRGSVLTTKGTIEMEASIMDSTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLA 120
            PHFNAGRGSVLTT GT+EMEASIMDSTKRCGAVSGLTTVVN ISLARLVMEKTPHIYL 
Sbjct: 61  NPHFNAGRGSVLTTNGTVEMEASIMDSTKRCGAVSGLTTVVNAISLARLVMEKTPHIYLG 120

Query: 121 FDGAEAFAREQGVETTNSSHFITPQNIERLKQAKEANRVQIDYTQPLPQATEQETENANG 180
           FDGAEAFAREQGVETT+SSHFITP+NIERLKQAKEANRVQIDYTQP+PQA  +ETE  NG
Sbjct: 121 FDGAEAFAREQGVETTDSSHFITPENIERLKQAKEANRVQIDYTQPIPQAPAEETEIPNG 180

Query: 181 DSQIGTVGCVAVDNHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAISATGKGE 240
           DSQ+GTVGCVAVDNHGNLA+ATSTGGLVNKMVGRIGDTP+IGAGTYAN+LCA+SATGKGE
Sbjct: 181 DSQLGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPVIGAGTYANNLCAVSATGKGE 240

Query: 241 AIIRGTVARDVAALMEFKGLSLEEATAYVVESVPKGNVGLVAVSASGEVAIPYNTTGMFR 300
           AIIRGTVARDVAALMEFKGLSL+EA AY VE+VP+GNVGLVAVSASGEV +P+NTTGMFR
Sbjct: 241 AIIRGTVARDVAALMEFKGLSLKEAAAYAVETVPRGNVGLVAVSASGEVTMPFNTTGMFR 300

Query: 301 ACATEDGYSEIAIWSSKND 320
           ACATEDGYSEIAIW SK+D
Sbjct: 301 ACATEDGYSEIAIWPSKHD 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|P30364|ASPG_LUPAN1.9e-13880.00Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1[more]
sp|Q9ZSD6|ASPG_LUPLU1.6e-13778.44Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1[more]
sp|P50287|ASPGA_ARATH6.0e-13780.38Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08... [more]
sp|P50288|ASPG_LUPAL3.9e-13678.13Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1[more]
sp|P30362|ASPG_LUPAR1.1e-12578.22Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2... [more]
Match NameE-valueIdentityDescription
AT5G08100.13.3e-13880.38N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein[more]
AT3G16150.12.6e-9858.82N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein[more]
AT4G00590.15.9e-1825.97N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein[more]
AT5G61540.12.8e-1226.35N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BPR4|A0A1S3BPR4_CUCME5.9e-16693.73isoaspartyl peptidase/L-asparaginase OS=Cucumis melo OX=3656 GN=LOC103492179 PE=... [more]
tr|A0A0A0LW19|A0A0A0LW19_CUCSA2.5e-16493.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G570100 PE=4 SV=1[more]
tr|A0A2H3ZD04|A0A2H3ZD04_PHODC7.6e-14580.94isoaspartyl peptidase/L-asparaginase 1-like OS=Phoenix dactylifera OX=42345 GN=L... [more]
tr|M5WUV5|M5WUV5_PRUPE1.3e-14483.60Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G209700 PE=4 SV=1[more]
tr|A0A2P4LGJ9|A0A2P4LGJ9_QUESU2.2e-14483.96Isoaspartyl peptidase/l-asparaginase OS=Quercus suber OX=58331 GN=CFP56_64533 PE... [more]
Match NameE-valueIdentityDescription
XP_008450657.19.0e-16693.73PREDICTED: isoaspartyl peptidase/L-asparaginase [Cucumis melo][more]
XP_004135623.13.8e-16493.42PREDICTED: isoaspartyl peptidase/L-asparaginase 1 [Cucumis sativus] >KGN66085.1 ... [more]
XP_023530268.16.2e-15991.46isoaspartyl peptidase/L-asparaginase 1 [Cucurbita pepo subsp. pepo][more]
XP_022933679.14.5e-15790.82isoaspartyl peptidase/L-asparaginase 1 [Cucurbita moschata][more]
XP_022156717.11.3e-15687.77isoaspartyl peptidase/L-asparaginase 1 [Momordica charantia][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
Vocabulary: INTERPRO
TermDefinition
IPR029055Ntn_hydrolases_N
IPR000246Peptidase_T2
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016540 protein autoprocessing
biological_process GO:0006522 alanine metabolic process
biological_process GO:0006528 asparagine metabolic process
biological_process GO:0006531 aspartate metabolic process
biological_process GO:0006508 proteolysis
biological_process GO:0008150 biological_process
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0004067 asparaginase activity
molecular_function GO:0008798 beta-aspartyl-peptidase activity
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001689Bhi02M001689mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.60.20.30coord: 186..317
e-value: 6.3E-50
score: 170.6
NoneNo IPR availablePANTHERPTHR10188:SF24ISOASPARTYL PEPTIDASE/L-ASPARAGINASE 1coord: 1..317
NoneNo IPR availableCDDcd04701Asparaginase_2coord: 4..307
e-value: 1.10172E-132
score: 380.268
IPR000246Peptidase T2, asparaginase 2PFAMPF01112Asparaginase_2coord: 3..314
e-value: 3.2E-114
score: 381.0
IPR000246Peptidase T2, asparaginase 2PANTHERPTHR10188L-ASPARAGINASEcoord: 1..317
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILYSSF56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 183..314
coord: 3..152

The following gene(s) are paralogous to this gene:

None