CSPI01G25450 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G25450
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Beta-1,3-glucanase
Location	Chr1 : 20934400 .. 20935731 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACCTTTTCCATTCCAATGCCAAAGGCCTTTCCACTTTGACCTTAATTTCTATTCTTTGTCTACTGCTACAAAATCTGCAAACCACAGGTTTCATTCTCCCTCCCTCTAAATATAATTCATAATTGTACGTTATTCTACAAATAAGTTGAAGAAAAAGAACACATAGCACATGGGAACCGTCTGAGTGGCATCTATATTGTTTTTTGTCAATACAGGCAACACTCAATAAAAAGTTTGTCATGTAATTGATATTAATTGTTGCACTGAATTATTGTCCTCTCTCTCTCTCTCTCTCTTTATATATATATATATATATATAAGTATTGATCAATTCATTTCCAAAAAATAATATTGATTTAGTTTATTTTGAATTTTATGTAGGTGCTCAAATCGGTGTCTGCTATGGACAACTTGGCAACGACCTACCATCTCCCGCAGAAGTTATCGACCTTTACAACCAGAACAACATCCAAAGAATGCGACTTTACGCTCCAAATCAAGATACTTTCAATGCCCTTCGAGGCTCCAGCATTGAGCTCATGCTTGGCCTTCCCAATGATCAGATTCAATCTATGGCAGCGACTCAAGACAACGCTAATGCATGGATTCAAGACAACATCCTCAACTTTGCCGACGTAAATTTCAAGTACATCGTCGTTGGAAACGAAATAAAAACCAACGAAGAAGCTGCGAGATTTCTCGTGCCCGCCATGCAAAATATCCAAAATGCAATTTCAGCTGTTGGTTTGCAAGGGCAAATCAAAGTTTCCACTGCGTTCCACACTGGAATTCTTTCAGCAGAATCCTTCCCTCCTTCACACGGCTCCTTTGACGCCAACTATCTTCCAATTCTCAACCCGACTATCCGCTTCCTTCTCGACAACAACTCCCCATTGCTCCTCAACTTGTACCCTTACTTTAGTTATGTAGCAACTCCAAACATGGAACTTGATTATGCAATTTTTACCGGGACATCGTTAGTTGAAGATGGGGAGTTCAATTATCAAAACTTGTTCGATGCTATTTTGGATACTGTTTATTCAGCTTTGGAGAAGAATGGTGGAGGATCATTGGAGGTTGTTGTATCCGAGACCGGTTGGCCGACGGAGGGTGGAGAGGCTGCGACGGTTGACAATGCAAGGACTTACAATAACAATCTAATTCAACATGTGAAACAAGGGACTCCAAAGAGACAAGGAAGGGCTATTGAAACTTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACTCCTCCGGAGGTTGAGAGACATTGGGGTTTGTTCTCTCCAAATAAGCAGCCTAAGTATCCAGTCAACTTCAAC

mRNA sequence

ATGGCCAACCTTTTCCATTCCAATGCCAAAGGCCTTTCCACTTTGACCTTAATTTCTATTCTTTGTCTACTGCTACAAAATCTGCAAACCACAGGTGCTCAAATCGGTGTCTGCTATGGACAACTTGGCAACGACCTACCATCTCCCGCAGAAGTTATCGACCTTTACAACCAGAACAACATCCAAAGAATGCGACTTTACGCTCCAAATCAAGATACTTTCAATGCCCTTCGAGGCTCCAGCATTGAGCTCATGCTTGGCCTTCCCAATGATCAGATTCAATCTATGGCAGCGACTCAAGACAACGCTAATGCATGGATTCAAGACAACATCCTCAACTTTGCCGACGTAAATTTCAAGTACATCGTCGTTGGAAACGAAATAAAAACCAACGAAGAAGCTGCGAGATTTCTCGTGCCCGCCATGCAAAATATCCAAAATGCAATTTCAGCTGTTGGTTTGCAAGGGCAAATCAAAGTTTCCACTGCGTTCCACACTGGAATTCTTTCAGCAGAATCCTTCCCTCCTTCACACGGCTCCTTTGACGCCAACTATCTTCCAATTCTCAACCCGACTATCCGCTTCCTTCTCGACAACAACTCCCCATTGCTCCTCAACTTGTACCCTTACTTTAGTTATGTAGCAACTCCAAACATGGAACTTGATTATGCAATTTTTACCGGGACATCGTTAGTTGAAGATGGGGAGTTCAATTATCAAAACTTGTTCGATGCTATTTTGGATACTGTTTATTCAGCTTTGGAGAAGAATGGTGGAGGATCATTGGAGGTTGTTGTATCCGAGACCGGTTGGCCGACGGAGGGTGGAGAGGCTGCGACGGTTGACAATGCAAGGACTTACAATAACAATCTAATTCAACATGTGAAACAAGGGACTCCAAAGAGACAAGGAAGGGCTATTGAAACTTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACTCCTCCGGAGGTTGAGAGACATTGGGGTTTGTTCTCTCCAAATAAGCAGCCTAAGTATCCAGTCAACTTCAAC

Coding sequence (CDS)

ATGGCCAACCTTTTCCATTCCAATGCCAAAGGCCTTTCCACTTTGACCTTAATTTCTATTCTTTGTCTACTGCTACAAAATCTGCAAACCACAGGTGCTCAAATCGGTGTCTGCTATGGACAACTTGGCAACGACCTACCATCTCCCGCAGAAGTTATCGACCTTTACAACCAGAACAACATCCAAAGAATGCGACTTTACGCTCCAAATCAAGATACTTTCAATGCCCTTCGAGGCTCCAGCATTGAGCTCATGCTTGGCCTTCCCAATGATCAGATTCAATCTATGGCAGCGACTCAAGACAACGCTAATGCATGGATTCAAGACAACATCCTCAACTTTGCCGACGTAAATTTCAAGTACATCGTCGTTGGAAACGAAATAAAAACCAACGAAGAAGCTGCGAGATTTCTCGTGCCCGCCATGCAAAATATCCAAAATGCAATTTCAGCTGTTGGTTTGCAAGGGCAAATCAAAGTTTCCACTGCGTTCCACACTGGAATTCTTTCAGCAGAATCCTTCCCTCCTTCACACGGCTCCTTTGACGCCAACTATCTTCCAATTCTCAACCCGACTATCCGCTTCCTTCTCGACAACAACTCCCCATTGCTCCTCAACTTGTACCCTTACTTTAGTTATGTAGCAACTCCAAACATGGAACTTGATTATGCAATTTTTACCGGGACATCGTTAGTTGAAGATGGGGAGTTCAATTATCAAAACTTGTTCGATGCTATTTTGGATACTGTTTATTCAGCTTTGGAGAAGAATGGTGGAGGATCATTGGAGGTTGTTGTATCCGAGACCGGTTGGCCGACGGAGGGTGGAGAGGCTGCGACGGTTGACAATGCAAGGACTTACAATAACAATCTAATTCAACATGTGAAACAAGGGACTCCAAAGAGACAAGGAAGGGCTATTGAAACTTATGTGTTTGCTATGTTTGATGAGAATGAGAAGACAACTCCTCCGGAGGTTGAGAGACATTGGGGTTTGTTCTCTCCAAATAAGCAGCCTAAGTATCCAGTCAACTTCAAC

Homology

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BLAST of CSPI01G25450 vs. Swiss-Prot
Match: E13B_PRUPE (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica GN=GNS1 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.4e-112
Identity = 209/341 (61.29%), Postives = 262/341 (76.83%), Query Frame = 1


Query: 15  LTLISILCLL-------LQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLY 74
           L+LISI+ LL       L   Q TGA IGVC G +G+DLP  AEV+ LY  NNI RMRLY
Sbjct: 13  LSLISIVLLLGQLVVGSLATKQHTGAPIGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLY 72

Query: 75  APNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVGNE 134
            PN     ALRGS+I+L+LG+PN+ +Q +A +Q NANAW+Q+N+ N+A+V FKYI VGNE
Sbjct: 73  DPNPAALEALRGSNIKLLLGVPNENLQYIALSQANANAWVQNNVRNYANVKFKYIAVGNE 132

Query: 135 IKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLP 194
           +K ++  A+FLVPAM+NIQ AIS  GL  +IKVSTA  TG+L  E+FPPS GSF + Y  
Sbjct: 133 VKPSDSFAQFLVPAMRNIQEAISLAGLAKKIKVSTAIDTGVL-GETFPPSIGSFKSEYNA 192

Query: 195 ILNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMELDYAIFTGTS-LVEDGEFNYQNLFDA 254
           +L P IRFL+ + SPLL+NLYPYF+Y   T ++ LDYA+FT  S +V+DG F Y+NLFDA
Sbjct: 193 LLYPIIRFLVSHQSPLLVNLYPYFAYSGNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDA 252

Query: 255 ILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGR 314
           +LD VY+ALEK GGGSL+VV+SETGWP+  G A T+DNART+ +NLIQHVK+GTP+R GR
Sbjct: 253 MLDGVYAALEKAGGGSLKVVISETGWPSAAGTATTIDNARTFISNLIQHVKEGTPRRPGR 312

Query: 315 AIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
            IETY+FAMFDEN KT  PE+E+HWGLFSP KQPKY ++FN
Sbjct: 313 PIETYIFAMFDENRKT--PELEKHWGLFSPTKQPKYQISFN 350

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BLAST of CSPI01G25450 vs. Swiss-Prot
Match: E13A_SOYBN (Glucan endo-1,3-beta-glucosidase OS=Glycine max PE=1 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.5e-109
Identity = 201/347 (57.93%), Postives = 263/347 (75.79%), Query Frame = 1


Query: 5   FHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRM 64
           +HS+ K  S   +  +  LL+    TT AQ GVCYG+LGN+LP+P EV+ LYNQ NI+RM
Sbjct: 4   YHSSGKSSSMTAIAFLFILLITYTGTTDAQSGVCYGRLGNNLPTPQEVVALYNQANIRRM 63

Query: 65  RLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFAD-VNFKYIV 124
           R+Y P+ +   ALRGS+IEL+L +PND ++++A++QDNAN W+QDNI N+A+ V F+Y+ 
Sbjct: 64  RIYGPSPEVLEALRGSNIELLLDIPNDNLRNLASSQDNANKWVQDNIKNYANNVRFRYVS 123

Query: 125 VGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDA 184
           VGNE+K     A+FLVPA++NIQ AIS  GL  Q+KVSTA  TG L AESFPPS GSF +
Sbjct: 124 VGNEVKPEHSFAQFLVPALENIQRAISNAGLGNQVKVSTAIDTGAL-AESFPPSKGSFKS 183

Query: 185 NYL-PILNPTIRFLLDNNSPLLLNLYPYFSYVATP-NMELDYAIFTGTSLV-EDGEFNYQ 244
           +Y    L+  IRFL++NN+PL++N+Y YF+Y A P ++ LDYA+F   S+V +DG   Y+
Sbjct: 184 DYRGAYLDGVIRFLVNNNAPLMVNVYSYFAYTANPKDISLDYALFRSPSVVVQDGSLGYR 243

Query: 245 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 304
           NLFDA +D VY+ALEK GGGSL +VVSE+GWP+ GG A ++DNARTYN NL+++VKQGTP
Sbjct: 244 NLFDASVDAVYAALEKAGGGSLNIVVSESGWPSSGGTATSLDNARTYNTNLVRNVKQGTP 303

Query: 305 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSP-NKQPKYPVNFN 347
           KR G  +ETYVFAMFDEN+K   PE E+ WGLFSP  KQPKY +NFN
Sbjct: 304 KRPGAPLETYVFAMFDENQK--QPEFEKFWGLFSPITKQPKYSINFN 347

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BLAST of CSPI01G25450 vs. Swiss-Prot
Match: BG1_ARATH (Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana GN=BG1 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 7.4e-109
Identity = 199/337 (59.05%), Postives = 255/337 (75.67%), Query Frame = 1


Query: 12  LSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQ 71
           L++LTL+  L L   N   TG Q+GVCYG+ GN+LPSPAE I L+ Q NIQR+RLY+P+ 
Sbjct: 6   LASLTLL--LGLFFVNTNPTGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDH 65

Query: 72  DTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFAD-VNFKYIVVGNEIKT 131
           D   ALRGS+IE+ LGLPN  +QS+A++Q  ANAW+Q  ++N+A+ V F+YI VGNE+K 
Sbjct: 66  DVLAALRGSNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKI 125

Query: 132 NEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILN 191
           ++  A+FLVPAM+NI  A+ A GL G+IKVST+   G+L  ES+PPS GSF  + + ++ 
Sbjct: 126 SDSYAQFLVPAMENIDRAVLAAGLGGRIKVSTSVDMGVLR-ESYPPSKGSFRGDVMVVME 185

Query: 192 PTIRFLLDNNSPLLLNLYPYFSYVATPN-MELDYAIFTGTS-LVEDGEFNYQNLFDAILD 251
           P IRFL+  NSPLLLNLY YFSY      + LDYA+FT  S +V D   +YQNLFDA+LD
Sbjct: 186 PIIRFLVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLD 245

Query: 252 TVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIE 311
            +YSALEK+GG SLE+VV+ETGWPT GG    ++NAR YNNNLI+HVK GTPKR G+ IE
Sbjct: 246 AMYSALEKSGGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIE 305

Query: 312 TYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNF 346
           TY+FA++DEN+K TPP VE+ WGLF PNKQPKY +NF
Sbjct: 306 TYLFAIYDENQKPTPPYVEKFWGLFYPNKQPKYDINF 339

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BLAST of CSPI01G25450 vs. Swiss-Prot
Match: E13H_TOBAC (Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.3e-99
Identity = 184/337 (54.60%), Postives = 247/337 (73.29%), Query Frame = 1


Query: 15  LTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQDTF 74
           +TL+ +  L L  L  TGAQ GVCYG+ GN LPSPA+V+ L N+NNI+RMR+Y P+Q T 
Sbjct: 6   VTLLLLSVLTLATLDFTGAQAGVCYGRQGNGLPSPADVVSLCNRNNIRRMRIYDPDQPTL 65

Query: 75  NALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEIKTNEEA 134
            ALRGS+IELMLG+PN  ++++AA+Q NA+ W+Q+N+ N+ +V F+YI VGNE+    E 
Sbjct: 66  EALRGSNIELMLGVPNPDLENVAASQANADTWVQNNVRNYGNVKFRYIAVGNEVSPLNEN 125

Query: 135 ARF---LVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNP 194
           +++   L+ AM+NIQ AIS  GL  QIKVSTA  TG L+ ++ PPS+G F  +    + P
Sbjct: 126 SKYVPVLLNAMRNIQTAISGAGLGNQIKVSTAIETG-LTTDTSPPSNGRFKDDVRQFIEP 185

Query: 195 TIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLV-EDGEFNYQNLFDAILDTV 254
            I FL+ N +PLL+NLYPYF+     +++L+YA+FT + +V  D    Y+NLFDAILD  
Sbjct: 186 IINFLVTNRAPLLVNLYPYFAIANNADIKLEYALFTSSEVVVNDNGRGYRNLFDAILDAT 245

Query: 255 YSALEKNGGGSLEVVVSETGWPTEG-GEAATVDNARTYNNNLIQHVKQGTPKRQGRAIET 314
           YSALEK  G SLE+VVSE+GWP+ G G+  ++DNARTYNNNLI HVK G+PKR    IET
Sbjct: 246 YSALEKASGSSLEIVVSESGWPSAGAGQLTSIDNARTYNNNLISHVKGGSPKRPSGPIET 305

Query: 315 YVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           YVFA+FDE++K   PE+E+H+GLFS N QPKY ++FN
Sbjct: 306 YVFALFDEDQKD--PEIEKHFGLFSANMQPKYQISFN 339

[Close]

BLAST of CSPI01G25450 vs. Swiss-Prot
Match: BG3_ARATH (Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana GN=BG3 PE=2 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 4.5e-98
Identity = 191/343 (55.69%), Postives = 245/343 (71.43%), Query Frame = 1


Query: 11  GLSTLTLISILCLLLQNL-----QTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMR 70
           G S L  + +L LLL  +      T   QIGVCYG+ GN+L   +EV+ LY Q NI+RMR
Sbjct: 6   GSSFLASLPLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMR 65

Query: 71  LYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVG 130
           LY PNQ+T NALRGS+IEL+L +PN  +Q +A++Q  A+ W+++N+ N+A+V F+YI VG
Sbjct: 66  LYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVG 125

Query: 131 NEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANY 190
           NE++ +++AA F++PAMQNI+ A+S++G    IKVSTA  T  +S   FPPS G+F   +
Sbjct: 126 NEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGIS--GFPPSSGTFTPEF 185

Query: 191 LPILNPTIRFLLDNNSPLLLNLYPYFSYVAT-PNMELDYAIFTGTS-LVEDGEFNYQNLF 250
              + P I FL    SPLL+N YPYFSY     ++ LDY +FT  S +V DG+  Y+NLF
Sbjct: 186 RSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLF 245

Query: 251 DAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQ 310
            AILDTVY++LEK GGGSLE+VVSE+GWPT GG A  VDNARTY NNLIQ VK G+P+R 
Sbjct: 246 HAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRP 305

Query: 311 GRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           GRA ETY+FAMFDEN K   PE E+ WGLF PN QPKY VNFN
Sbjct: 306 GRATETYIFAMFDENSK-QGPETEKFWGLFLPNLQPKYVVNFN 341

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BLAST of CSPI01G25450 vs. TrEMBL
Match: A0A0A0LYQ8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 1.3e-192
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 1


Query: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN
Sbjct: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120
           IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK
Sbjct: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120

Query: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240
           FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ
Sbjct: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240

Query: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP
Sbjct: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK 341
           KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK
Sbjct: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK 340

[Close]

BLAST of CSPI01G25450 vs. TrEMBL
Match: G8Z9V4_9ROSA (Beta-1,3-glucanase OS=Malus hupehensis PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 6.7e-125
Identity = 226/340 (66.47%), Postives = 277/340 (81.47%), Query Frame = 1


Query: 9   AKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYA 68
           A+G S +++I +L  L+ + +TTGAQIGVCYG  G DLP  AEVI LY Q NI+RMRLY 
Sbjct: 11  ARGPSVISIILVLGQLMASFETTGAQIGVCYGMNG-DLPPQAEVIALYKQKNIRRMRLYD 70

Query: 69  PNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEI 128
           PN     ALRGS IELMLGLPNDQ+Q++A++Q NAN W+Q+N+ N+A+V FKYI VGNEI
Sbjct: 71  PNPAALAALRGSDIELMLGLPNDQLQNIASSQANANTWVQNNVRNYANVRFKYIAVGNEI 130

Query: 129 KTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPI 188
           K ++ +A+FLVPAM+NIQNAIS+ GL  QIKVSTA  TG+L   SFPPS G F  +Y PI
Sbjct: 131 KPSDSSAQFLVPAMRNIQNAISSAGLGNQIKVSTAIDTGVL-GNSFPPSKGEFRGDYSPI 190

Query: 189 LNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMELDYAIFTGTS-LVEDGEFNYQNLFDAI 248
           LNP +RFL+DN SPLL N+YPYFSY+  T ++ LDYA+FT TS +V+DG+F Y+NLFDAI
Sbjct: 191 LNPVVRFLVDNKSPLLDNMYPYFSYIGNTRDIRLDYALFTATSVVVQDGQFGYRNLFDAI 250

Query: 249 LDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRA 308
           LD VY+AL+K GGGSLE+V+SE+GWPT GG A TVDNARTYN+NL+QHVK GTP++ GR 
Sbjct: 251 LDAVYAALDKIGGGSLEIVISESGWPTAGGTATTVDNARTYNSNLVQHVKGGTPRKPGRP 310

Query: 309 IETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           IETY+FAMFDEN KT  PE+E+HWGLFSPNKQPKYP+NFN
Sbjct: 311 IETYIFAMFDENRKT--PELEKHWGLFSPNKQPKYPINFN 346

[Close]

BLAST of CSPI01G25450 vs. TrEMBL
Match: G8XUV8_9ROSA (Beta-1,3-glucanase OS=Malus hupehensis PE=2 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 3.1e-122
Identity = 223/340 (65.59%), Postives = 274/340 (80.59%), Query Frame = 1


Query: 9   AKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYA 68
           A+G S +++I +L  L+ + +TTGAQIGVCYG  G DLP  AEVI LY Q NI+RMRLY 
Sbjct: 11  ARGPSVISIILVLGQLMASFETTGAQIGVCYGMNG-DLPPQAEVIALYKQKNIRRMRLYD 70

Query: 69  PNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEI 128
           P+     ALRGS  ELMLG+PNDQ+Q++A++Q NAN W+Q+N+ N+A+V FKYI VGNEI
Sbjct: 71  PSPAALAALRGSDTELMLGIPNDQLQNIASSQANANTWVQNNVRNYANVRFKYIAVGNEI 130

Query: 129 KTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPI 188
           K ++ +A+FLVPAM+NIQNAIS+ GL  QIKVSTA  TG+L   SFPPS G F  +Y PI
Sbjct: 131 KPSDSSAQFLVPAMRNIQNAISSAGLGNQIKVSTAIDTGVL-GNSFPPSKGEFRGDYSPI 190

Query: 189 LNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMELDYAIFTGTS-LVEDGEFNYQNLFDAI 248
           LNP +RFL+DN SPLL+NLYPYFSY+  T ++ LDYA+FT  S +V+DGE  Y+NLFDAI
Sbjct: 191 LNPVVRFLVDNKSPLLVNLYPYFSYIGNTRDIRLDYALFTAQSVVVQDGERGYRNLFDAI 250

Query: 249 LDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRA 308
           L  VY+AL+K GGGSLE+VVSE+GWPT GG A TVDNARTYN+NL+QHVK GTP++ GR 
Sbjct: 251 LGAVYAALDKVGGGSLEIVVSESGWPTAGGTATTVDNARTYNSNLVQHVKGGTPRKPGRP 310

Query: 309 IETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           IETY+FAMFDEN KT  PE+E+HWGLFSPNKQPKYP+NFN
Sbjct: 311 IETYIFAMFDENRKT--PELEKHWGLFSPNKQPKYPINFN 346

[Close]

BLAST of CSPI01G25450 vs. TrEMBL
Match: Q944B1_PRUPE (Beta-1,3-glucanase OS=Prunus persica GN=Gns3 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.3e-120
Identity = 222/348 (63.79%), Postives = 277/348 (79.60%), Query Frame = 1


Query: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MA    S  +G S ++++ +L LL+ + +TTGAQIGVCYG LG+ LP P+EVI LY QNN
Sbjct: 1   MAKSNSSVGRGPSMISIVFLLGLLMASFETTGAQIGVCYGMLGDRLPPPSEVIALYKQNN 60

Query: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120
           I+RMRLY PNQ    AL+GS IELMLG+PND +QS+A++Q NAN W+Q+N+ N+ +V FK
Sbjct: 61  IRRMRLYDPNQAALAALKGSYIELMLGVPNDNLQSLASSQANANTWVQNNVRNYGNVRFK 120

Query: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180
           YI VGNE+K ++  A+FLVPAMQNIQNAIS+ GL   IKVSTA  TG+L   SFPPS G 
Sbjct: 121 YIAVGNEVKPSDSYAQFLVPAMQNIQNAISSAGL--GIKVSTAVDTGVL-GNSFPPSKGE 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMELDYAIFTGTS-LVEDGEFN 240
           F + Y  +LNP IRFL++N SPLL+NLYPYFSY + T ++ LDYA+FT  S +V+DG+  
Sbjct: 181 FKSEYGALLNPIIRFLVNNRSPLLVNLYPYFSYSSNTHDIRLDYALFTAPSVVVQDGQRG 240

Query: 241 YQNLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQG 300
           Y+NLFDAILD VY+ALEK GGGSLE+V+SE+GWP+ GG A T+DNARTYN NLIQHVK G
Sbjct: 241 YRNLFDAILDAVYAALEKAGGGSLEIVISESGWPSAGGTATTIDNARTYNANLIQHVKGG 300

Query: 301 TPKRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           TP++ GRAIETY+FAMFDEN K   PE+E+HWGLFSP+KQPKYP+NFN
Sbjct: 301 TPRKPGRAIETYIFAMFDENRKN--PELEKHWGLFSPSKQPKYPINFN 343

[Close]

BLAST of CSPI01G25450 vs. TrEMBL
Match: W9R6M6_9ROSA (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Morus notabilis GN=L484_016574 PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.8e-117
Identity = 213/337 (63.20%), Postives = 266/337 (78.93%), Query Frame = 1


Query: 12  LSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQ 71
           +ST+ L+ +L ++   +  T AQIGVCYG LGN+LPS  EVI LYNQNNI+RMRLY PNQ
Sbjct: 15  ISTMLLLGLL-IITSTIDKTAAQIGVCYGMLGNNLPSQKEVIALYNQNNIRRMRLYDPNQ 74

Query: 72  DTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEIKTN 131
               ALRG++IELMLG+PN  +Q +A+ Q  AN W+Q+N+ N+ +V FKYI VGNE+KT+
Sbjct: 75  AALQALRGTNIELMLGVPNTDLQRLASNQAEANTWVQNNVRNYNNVKFKYIAVGNEVKTS 134

Query: 132 EEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILNP 191
           +  A+FLVPAMQNIQ AIS  GL  QIKVST+  TG+L   S+PPS GSF ++Y  I+NP
Sbjct: 135 DSFAQFLVPAMQNIQRAISTFGLANQIKVSTSIDTGVLDV-SYPPSKGSFKSDYRAIINP 194

Query: 192 TIRFLLDNNSPLLLNLYPYFSYVA-TPNMELDYAIFTGTS-LVEDGEFNYQNLFDAILDT 251
            I FL+ N SPLL+NLYPYFSY   T ++ LDYA+F   S +V+DG++ Y+NLFDAILD 
Sbjct: 195 VISFLVSNGSPLLVNLYPYFSYSGNTRDISLDYALFRSPSVVVQDGQYGYRNLFDAILDA 254

Query: 252 VYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIET 311
           VYSALEK GGGSLE+VVSE+GWP+ GG   TVDNARTYNNNL+QHVK+GTPK+ GR I+T
Sbjct: 255 VYSALEKAGGGSLEIVVSESGWPSAGGTGTTVDNARTYNNNLVQHVKKGTPKKPGRPIQT 314

Query: 312 YVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           Y+FAMF+EN+KT  PE+E+HWGLF PNKQPKY +NFN
Sbjct: 315 YIFAMFNENQKT--PELEKHWGLFLPNKQPKYQMNFN 347

[Close]

BLAST of CSPI01G25450 vs. TAIR10
Match: AT3G57270.1 (AT3G57270.1 beta-1,3-glucanase 1)

HSP 1 Score: 395.2 bits (1014), Expect = 4.1e-110
Identity = 199/337 (59.05%), Postives = 255/337 (75.67%), Query Frame = 1


Query: 12  LSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQ 71
           L++LTL+  L L   N   TG Q+GVCYG+ GN+LPSPAE I L+ Q NIQR+RLY+P+ 
Sbjct: 6   LASLTLL--LGLFFVNTNPTGGQVGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDH 65

Query: 72  DTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFAD-VNFKYIVVGNEIKT 131
           D   ALRGS+IE+ LGLPN  +QS+A++Q  ANAW+Q  ++N+A+ V F+YI VGNE+K 
Sbjct: 66  DVLAALRGSNIEVTLGLPNSYLQSVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKI 125

Query: 132 NEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILN 191
           ++  A+FLVPAM+NI  A+ A GL G+IKVST+   G+L  ES+PPS GSF  + + ++ 
Sbjct: 126 SDSYAQFLVPAMENIDRAVLAAGLGGRIKVSTSVDMGVLR-ESYPPSKGSFRGDVMVVME 185

Query: 192 PTIRFLLDNNSPLLLNLYPYFSYVATPN-MELDYAIFTGTS-LVEDGEFNYQNLFDAILD 251
           P IRFL+  NSPLLLNLY YFSY      + LDYA+FT  S +V D   +YQNLFDA+LD
Sbjct: 186 PIIRFLVSKNSPLLLNLYTYFSYAGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLD 245

Query: 252 TVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRAIE 311
            +YSALEK+GG SLE+VV+ETGWPT GG    ++NAR YNNNLI+HVK GTPKR G+ IE
Sbjct: 246 AMYSALEKSGGASLEIVVAETGWPTGGGTDTNIENARIYNNNLIKHVKNGTPKRPGKEIE 305

Query: 312 TYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNF 346
           TY+FA++DEN+K TPP VE+ WGLF PNKQPKY +NF
Sbjct: 306 TYLFAIYDENQKPTPPYVEKFWGLFYPNKQPKYDINF 339

[Close]

BLAST of CSPI01G25450 vs. TAIR10
Match: AT3G57240.1 (AT3G57240.1 beta-1,3-glucanase 3)

HSP 1 Score: 359.4 bits (921), Expect = 2.5e-99
Identity = 191/343 (55.69%), Postives = 245/343 (71.43%), Query Frame = 1


Query: 11  GLSTLTLISILCLLLQNL-----QTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMR 70
           G S L  + +L LLL  +      T   QIGVCYG+ GN+L   +EV+ LY Q NI+RMR
Sbjct: 6   GSSFLASLPLLLLLLSFILASFFDTAVGQIGVCYGRNGNNLRPASEVVALYQQRNIRRMR 65

Query: 71  LYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVG 130
           LY PNQ+T NALRGS+IEL+L +PN  +Q +A++Q  A+ W+++N+ N+A+V F+YI VG
Sbjct: 66  LYDPNQETLNALRGSNIELVLDVPNPDLQRLASSQAEADTWVRNNVRNYANVTFRYISVG 125

Query: 131 NEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANY 190
           NE++ +++AA F++PAMQNI+ A+S++G    IKVSTA  T  +S   FPPS G+F   +
Sbjct: 126 NEVQPSDQAASFVLPAMQNIERAVSSLG----IKVSTAIDTRGIS--GFPPSSGTFTPEF 185

Query: 191 LPILNPTIRFLLDNNSPLLLNLYPYFSYVAT-PNMELDYAIFTGTS-LVEDGEFNYQNLF 250
              + P I FL    SPLL+N YPYFSY     ++ LDY +FT  S +V DG+  Y+NLF
Sbjct: 186 RSFIAPVISFLSSKQSPLLVNNYPYFSYTGNMRDIRLDYTLFTAPSTVVNDGQNQYRNLF 245

Query: 251 DAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQ 310
            AILDTVY++LEK GGGSLE+VVSE+GWPT GG A  VDNARTY NNLIQ VK G+P+R 
Sbjct: 246 HAILDTVYASLEKAGGGSLEIVVSESGWPTAGGAATGVDNARTYVNNLIQTVKNGSPRRP 305

Query: 311 GRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           GRA ETY+FAMFDEN K   PE E+ WGLF PN QPKY VNFN
Sbjct: 306 GRATETYIFAMFDENSK-QGPETEKFWGLFLPNLQPKYVVNFN 341

[Close]

BLAST of CSPI01G25450 vs. TAIR10
Match: AT3G57260.1 (AT3G57260.1 beta-1,3-glucanase 2)

HSP 1 Score: 350.9 bits (899), Expect = 9.0e-97
Identity = 186/345 (53.91%), Postives = 245/345 (71.01%), Query Frame = 1


Query: 7   SNAKGLSTLTLISILCLLL--QNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRM 66
           S ++ L++  ++ IL  L+       T  QIGVCYG LG+ LPSP++V+ LY Q NIQRM
Sbjct: 2   SESRSLASPPMLMILLSLVIASFFNHTAGQIGVCYGMLGDTLPSPSDVVALYKQQNIQRM 61

Query: 67  RLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFAD-VNFKYIV 126
           RLY P+     ALRGS IEL+L +P+  ++ +A++Q  A+ W+Q+N+ ++ D V F+YI 
Sbjct: 62  RLYGPDPGALAALRGSDIELILDVPSSDLERLASSQTEADKWVQENVQSYRDGVRFRYIN 121

Query: 127 VGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDA 186
           VGNE+K +     FL+ AMQNI+NA+S  GL  ++KVSTA  T   + ++ PPS G F  
Sbjct: 122 VGNEVKPS--VGGFLLQAMQNIENAVSGAGL--EVKVSTAIATD-TTTDTSPPSQGRFRD 181

Query: 187 NYLPILNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMELDYAIFTGTSLVE-DGEFNYQN 246
            Y   L P I FL    SPLL+NLYPYFSY+  T N+ LDYA+FT  S V+ D  ++YQN
Sbjct: 182 EYKSFLEPVIGFLASKQSPLLVNLYPYFSYMGDTANIHLDYALFTAQSTVDNDPGYSYQN 241

Query: 247 LFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPK 306
           LFDA LD+VY+ALEK+GGGSLE+VVSETGWPTEG    +V+NA+TY NNLIQHVK G+P+
Sbjct: 242 LFDANLDSVYAALEKSGGGSLEIVVSETGWPTEGAVGTSVENAKTYVNNLIQHVKNGSPR 301

Query: 307 RQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           R G+AIETY+FAMFDEN+K   P  E+ WGLF P++Q KY VNFN
Sbjct: 302 RPGKAIETYIFAMFDENKK--EPTYEKFWGLFHPDRQSKYEVNFN 339

[Close]

BLAST of CSPI01G25450 vs. TAIR10
Match: AT4G16260.1 (AT4G16260.1 Glycosyl hydrolase superfamily protein)

HSP 1 Score: 325.1 bits (832), Expect = 5.3e-89
Identity = 170/340 (50.00%), Postives = 238/340 (70.00%), Query Frame = 1


Query: 12  LSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYAPNQ 71
           ++TL L+  L +      T+G  +GVCYG +GN+LPS ++ I L+ QNNI+R+RLY PNQ
Sbjct: 1   MTTLFLLIALFITTILNPTSGESVGVCYGMMGNNLPSQSDTIALFRQNNIRRVRLYDPNQ 60

Query: 72  DTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILN-FADVNFKYIVVGNEIKT 131
              NALR + IE+++G+PN  ++S+     +A +W+Q+N+LN +  V+FKYI VGNE+  
Sbjct: 61  AALNALRNTGIEVIIGVPNTDLRSL-TNPSSARSWLQNNVLNYYPAVSFKYIAVGNEVSP 120

Query: 132 NEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPILN 191
           +      ++PAM+N+ +A+    LQ +IKVSTA    ++   SFPPS G F  +    ++
Sbjct: 121 S-NGGDVVLPAMRNVYDALRGANLQDRIKVSTAIDMTLI-GNSFPPSSGEFRGDVRWYID 180

Query: 192 PTIRFLLDNNSPLLLNLYPYFSYVATP-NMELDYAIFTGTSLVE-DGEFNYQNLFDAILD 251
           P I FL   NS LL N+YPYFSYV  P ++ L YA+FT  S+V  DG   YQNLFDA+LD
Sbjct: 181 PVIGFLTSTNSALLANIYPYFSYVDNPRDISLSYALFTSPSVVVWDGSRGYQNLFDALLD 240

Query: 252 TVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQ--GTPKRQGRA 311
            VYSA+E++GGGSL VVVSE+GWP+ GG AA+ DNAR +  NL   V++  GTPKR GR 
Sbjct: 241 VVYSAVERSGGGSLPVVVSESGWPSNGGNAASFDNARAFYTNLASRVRENRGTPKRPGRG 300

Query: 312 IETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           +ETY+FAMFDEN+K+  PE+E+++GLF PNKQPK+P+ F+
Sbjct: 301 VETYLFAMFDENQKS--PEIEKNFGLFFPNKQPKFPITFS 335

[Close]

BLAST of CSPI01G25450 vs. TAIR10
Match: AT5G56590.1 (AT5G56590.1 O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 260.4 bits (664), Expect = 1.6e-69
Identity = 144/349 (41.26%), Postives = 220/349 (63.04%), Query Frame = 1


Query: 9   AKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYA 68
           A+    +  ISIL LLL      G ++GVCYG+  +DLP+P++V+ L  Q+NI+ +R+Y 
Sbjct: 2   ARDFKLIFSISILLLLLDCCY--GGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYD 61

Query: 69  PNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNIL-NFADVNFKYIVVGNE 128
            N     A   +SIELM+G+PN  + + + +Q N + W+++++L  +      YI VG E
Sbjct: 62  YNSQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAE 121

Query: 129 IKTNE--EAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANY 188
              +    A+ F+VPAMQN+  A+  VGL  +IKVST    GILS  SFPPS G+F+++Y
Sbjct: 122 STDDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILS-RSFPPSAGAFNSSY 181

Query: 189 LPILNPTIRFLLDNNSPLLLNLYPYFSYVATP-NMELDYAIFTGTSLVEDGEFN--YQNL 248
              L P + FL +N SP +++LYPY++Y  +P N+ LDY +F  +S V D      Y+N+
Sbjct: 182 AYFLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNM 241

Query: 249 FDAILDTVYSALEKNGGGSLEVVVSETGWPTEGG----EAATVDNARTYNNNLIQHV--K 308
           FDA +D +Y AL      +++++V+ETGWPT+G      AA+ DNA TYN+N+I+HV   
Sbjct: 242 FDAQVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTN 301

Query: 309 QGTPKRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNF 346
           QGTP + G A+  Y+F++F+EN K    + ER+WGLF P++   Y ++F
Sbjct: 302 QGTPAKPGEAMNVYIFSLFNENRK-AGLDSERNWGLFYPDQTSVYQLDF 346

[Close]

BLAST of CSPI01G25450 vs. NCBI nr
Match: gi|778662097|ref|XP_011659353.1| (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis sativus])

HSP 1 Score: 694.1 bits (1790), Expect = 1.2e-196
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 1


Query: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN
Sbjct: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120
           IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK
Sbjct: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120

Query: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240
           FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ
Sbjct: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240

Query: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP
Sbjct: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN
Sbjct: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 346

[Close]

BLAST of CSPI01G25450 vs. NCBI nr
Match: gi|700210876|gb|KGN65972.1| (hypothetical protein Csa_1G555080 [Cucumis sativus])

HSP 1 Score: 680.2 bits (1754), Expect = 1.8e-192
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 1


Query: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN
Sbjct: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120
           IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK
Sbjct: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120

Query: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240
           FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ
Sbjct: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240

Query: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP
Sbjct: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK 341
           KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK
Sbjct: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK 340

[Close]

BLAST of CSPI01G25450 vs. NCBI nr
Match: gi|659118414|ref|XP_008459107.1| (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo])

HSP 1 Score: 643.3 bits (1658), Expect = 2.5e-181
Identity = 321/346 (92.77%), Postives = 333/346 (96.24%), Query Frame = 1


Query: 1   MANLFHSNAKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60
           MANLF S+ KGLSTLTLISILCLLL+NLQTT AQIGVCYGQLGNDLPSPAEVIDLYNQNN
Sbjct: 1   MANLFPSSDKGLSTLTLISILCLLLKNLQTTVAQIGVCYGQLGNDLPSPAEVIDLYNQNN 60

Query: 61  IQRMRLYAPNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFK 120
           IQRMRLYAPNQ TFNALRGSSIELMLGLPND+IQSMAATQDNANAW+QDNILNF DVNFK
Sbjct: 61  IQRMRLYAPNQATFNALRGSSIELMLGLPNDEIQSMAATQDNANAWVQDNILNFPDVNFK 120

Query: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGS 180
           YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGL G+IKVSTAFHTGILS ESFPPSHGS
Sbjct: 121 YIVVGNEIKTNEEAARFLVPAMQNIQNAISAVGLGGKIKVSTAFHTGILSVESFPPSHGS 180

Query: 181 FDANYLPILNPTIRFLLDNNSPLLLNLYPYFSYVATPNMELDYAIFTGTSLVEDGEFNYQ 240
           FDA+YLPILNPTIRFLLDNNSPLL+NLYPYFSYV TP+MELDYAIFTGTSLV DGEFNYQ
Sbjct: 181 FDASYLPILNPTIRFLLDNNSPLLVNLYPYFSYVGTPSMELDYAIFTGTSLVVDGEFNYQ 240

Query: 241 NLFDAILDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTP 300
           NLF AILDTVYSALEKNGGGSLE+VVSETGWPT+GGEAATVDNARTY NNLIQ VKQGTP
Sbjct: 241 NLFHAILDTVYSALEKNGGGSLEIVVSETGWPTDGGEAATVDNARTYTNNLIQRVKQGTP 300

Query: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPK+ V+FN
Sbjct: 301 KRQGRAIETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKFSVSFN 346

[Close]

BLAST of CSPI01G25450 vs. NCBI nr
Match: gi|313600351|gb|ADR71671.1| (beta-1,3-glucanase [Malus hupehensis])

HSP 1 Score: 455.3 bits (1170), Expect = 9.6e-125
Identity = 226/340 (66.47%), Postives = 277/340 (81.47%), Query Frame = 1


Query: 9   AKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYA 68
           A+G S +++I +L  L+ + +TTGAQIGVCYG  G DLP  AEVI LY Q NI+RMRLY 
Sbjct: 11  ARGPSVISIILVLGQLMASFETTGAQIGVCYGMNG-DLPPQAEVIALYKQKNIRRMRLYD 70

Query: 69  PNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEI 128
           PN     ALRGS IELMLGLPNDQ+Q++A++Q NAN W+Q+N+ N+A+V FKYI VGNEI
Sbjct: 71  PNPAALAALRGSDIELMLGLPNDQLQNIASSQANANTWVQNNVRNYANVRFKYIAVGNEI 130

Query: 129 KTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPI 188
           K ++ +A+FLVPAM+NIQNAIS+ GL  QIKVSTA  TG+L   SFPPS G F  +Y PI
Sbjct: 131 KPSDSSAQFLVPAMRNIQNAISSAGLGNQIKVSTAIDTGVL-GNSFPPSKGEFRGDYSPI 190

Query: 189 LNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMELDYAIFTGTS-LVEDGEFNYQNLFDAI 248
           LNP +RFL+DN SPLL N+YPYFSY+  T ++ LDYA+FT TS +V+DG+F Y+NLFDAI
Sbjct: 191 LNPVVRFLVDNKSPLLDNMYPYFSYIGNTRDIRLDYALFTATSVVVQDGQFGYRNLFDAI 250

Query: 249 LDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRA 308
           LD VY+AL+K GGGSLE+V+SE+GWPT GG A TVDNARTYN+NL+QHVK GTP++ GR 
Sbjct: 251 LDAVYAALDKIGGGSLEIVISESGWPTAGGTATTVDNARTYNSNLVQHVKGGTPRKPGRP 310

Query: 309 IETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           IETY+FAMFDEN KT  PE+E+HWGLFSPNKQPKYP+NFN
Sbjct: 311 IETYIFAMFDENRKT--PELEKHWGLFSPNKQPKYPINFN 346

[Close]

BLAST of CSPI01G25450 vs. NCBI nr
Match: gi|658032263|ref|XP_008351633.1| (PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Malus domestica])

HSP 1 Score: 454.1 bits (1167), Expect = 2.1e-124
Identity = 225/340 (66.18%), Postives = 279/340 (82.06%), Query Frame = 1


Query: 9   AKGLSTLTLISILCLLLQNLQTTGAQIGVCYGQLGNDLPSPAEVIDLYNQNNIQRMRLYA 68
           A+G S +++I +L  L+ + +TTGAQIGVCYG  G DLP  AEVI LY QNNI+RMRLY 
Sbjct: 11  ARGPSVISIILVLGQLMASFETTGAQIGVCYGMNG-DLPPQAEVIALYKQNNIRRMRLYD 70

Query: 69  PNQDTFNALRGSSIELMLGLPNDQIQSMAATQDNANAWIQDNILNFADVNFKYIVVGNEI 128
           PN     ALRGS IELMLG+PNDQ+Q++A++Q NAN W+Q+N+ N+A+V FKYI VGNEI
Sbjct: 71  PNPAALAALRGSDIELMLGIPNDQLQNIASSQANANTWVQNNVRNYANVRFKYIAVGNEI 130

Query: 129 KTNEEAARFLVPAMQNIQNAISAVGLQGQIKVSTAFHTGILSAESFPPSHGSFDANYLPI 188
           K ++ +A+FLVPAM+NIQNAIS+ GL  QIKVSTA  TG+L  +SFPPS+G F  +Y PI
Sbjct: 131 KPSDSSAQFLVPAMRNIQNAISSAGLGNQIKVSTAIDTGVL-GKSFPPSNGEFRGDYSPI 190

Query: 189 LNPTIRFLLDNNSPLLLNLYPYFSYVA-TPNMELDYAIFTGTS-LVEDGEFNYQNLFDAI 248
           LNP +RFL+DN SPLL+NLYPYFSY+  T ++ LDYA+FT  S +V+DG+  Y+NLFDAI
Sbjct: 191 LNPVVRFLVDNKSPLLVNLYPYFSYIGNTRDIRLDYALFTARSVVVQDGQRGYRNLFDAI 250

Query: 249 LDTVYSALEKNGGGSLEVVVSETGWPTEGGEAATVDNARTYNNNLIQHVKQGTPKRQGRA 308
           LD VY+AL+K GGGSL++VVSE+GWPT GG A TVDNARTYN+NL+QHVK GTP++ GR 
Sbjct: 251 LDAVYAALDKVGGGSLDIVVSESGWPTAGGTATTVDNARTYNSNLVQHVKGGTPRKPGRP 310

Query: 309 IETYVFAMFDENEKTTPPEVERHWGLFSPNKQPKYPVNFN 347
           IETY+FAMFDEN KT  PE+E+HWGLFSPNKQPKYP+NFN
Sbjct: 311 IETYIFAMFDENRKT--PELEKHWGLFSPNKQPKYPINFN 346

The following BLAST results are available for this feature:

BLAST of CSPI01G25450 vs. Swiss-Prot


Match Name	E-value	Identity	Description
E13B_PRUPE	1.4e-112	61.29	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica GN=GNS1 PE=3 S...	[more]
E13A_SOYBN	1.5e-109	57.93	Glucan endo-1,3-beta-glucosidase OS=Glycine max PE=1 SV=1	[more]
BG1_ARATH	7.4e-109	59.05	Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana GN=BG1 PE=...	[more]
E13H_TOBAC	5.3e-99	54.60	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-Q' OS=Nicotiana tabacum PE=1...	[more]
BG3_ARATH	4.5e-98	55.69	Probable glucan endo-1,3-beta-glucosidase BG3 OS=Arabidopsis thaliana GN=BG3 PE=...	[more]

BLAST of CSPI01G25450 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYQ8_CUCSA	1.3e-192	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G555080 PE=3 SV=1	[more]
G8Z9V4_9ROSA	6.7e-125	66.47	Beta-1,3-glucanase OS=Malus hupehensis PE=3 SV=1	[more]
G8XUV8_9ROSA	3.1e-122	65.59	Beta-1,3-glucanase OS=Malus hupehensis PE=2 SV=1	[more]
Q944B1_PRUPE	1.3e-120	63.79	Beta-1,3-glucanase OS=Prunus persica GN=Gns3 PE=3 SV=1	[more]
W9R6M6_9ROSA	1.8e-117	63.20	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Morus notabilis GN=L484_01657...	[more]

BLAST of CSPI01G25450 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G57270.1	4.1e-110	59.05	beta-1,3-glucanase 1	[more]
AT3G57240.1	2.5e-99	55.69	beta-1,3-glucanase 3	[more]
AT3G57260.1	9.0e-97	53.91	beta-1,3-glucanase 2	[more]
AT4G16260.1	5.3e-89	50.00	Glycosyl hydrolase superfamily protein	[more]
AT5G56590.1	1.6e-69	41.26	O-Glycosyl hydrolases family 17 protein	[more]

BLAST of CSPI01G25450 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778662097\|ref\|XP_011659353.1\|	1.2e-196	100.00	PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis sativus...	[more]
gi\|700210876\|gb\|KGN65972.1\|	1.8e-192	100.00	hypothetical protein Csa_1G555080 [Cucumis sativus]	[more]
gi\|659118414\|ref\|XP_008459107.1\|	2.5e-181	92.77	PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Cucumis melo]	[more]
gi\|313600351\|gb\|ADR71671.1\|	9.6e-125	66.47	beta-1,3-glucanase [Malus hupehensis]	[more]
gi\|658032263\|ref\|XP_008351633.1\|	2.1e-124	66.18	PREDICTED: glucan endo-1,3-beta-glucosidase, basic isoform-like [Malus domestica...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000490	Glyco_hydro_17
IPR013781	Glycoside hydrolase, catalytic domain
IPR017853	Glycoside_hydrolase_SF

Vocabulary: **Molecular Function**
Term	Definition
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0031515	tRNA (m1A) methyltransferase complex
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0016429	tRNA (adenine-N1-)-methyltransferase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G25450.1	CSPI01G25450.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 35..345 score: 1.0E
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 262..275 scor
IPR013781	Glycoside hydrolase, catalytic domain	GENE3D	G3DSA:3.20.20.80		coord: 35..345 score: 2.5E
IPR017853	Glycoside hydrolase superfamily	unknown	SSF51445	(Trans)glycosidases	coord: 35..344 score: 3.54
None	No IPR available	PANTHER	PTHR32227	FAMILY NOT NAMED	coord: 29..346 score: 1.5E
None	No IPR available	PANTHER	PTHR32227:SF49	BETA-1,3-GLUCANASE 1-RELATED	coord: 29..346 score: 1.5E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G25450	Cla009170	Watermelon (97103) v1	cpiwmB048
CSPI01G25450	Csa1G555080	Cucumber (Chinese Long) v2	cpicuB001
CSPI01G25450	ClCG01G008910	Watermelon (Charleston Gray)	cpiwcgB020
CSPI01G25450	Lsi02G010070	Bottle gourd (USVL1VR-Ls)	cpilsiB022
CSPI01G25450	MELO3C035674.2	Melon (DHL92) v3.6.1	cpimedB027
CSPI01G25450	CsaV3_1G037780	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G25450	Bhi08G000790	Wax gourd	cpiwgoB054
CSPI01G25450	Cucsa.167580	Cucumber (Gy14) v1	cgycpiB262
CSPI01G25450	CsGy1G024850	Cucumber (Gy14) v2	cgybcpiB002

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G25450	Watermelon (97103) v2	cpiwmbB006
CSPI01G25450	Cucurbita moschata (Rifu)	cmocpiB045
CSPI01G25450	Melon (DHL92) v3.5.1	cpimeB028
CSPI01G25450	Watermelon (Charleston Gray)	cpiwcgB042
CSPI01G25450	Watermelon (97103) v1	cpiwmB015