CSPI01G25330 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI01G25330
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionPhloem lectin
LocationChr1 : 20775618 .. 20777866 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACACCACTCTCATCTTCATCCATATCTCTACTCTCTTACTATCCAAGTTAACCTCTTGAAATGGTTTCCTCAGAGCTTGAAGCAAGAGAGAACTCGGAAACAAAAGAGAAATATGGTCATTGCTTGAGTTATATTTTGCCAACAACTGGAGATCATGAGGTGCACTGGCCCTCCCCCTCTGAGCTCTATAAACAACTTTGTGATGGGACTTCCCTCAACAATGGATCTAAGGCAAGTGTAACATTATTTTTAATTTCTTTTAGAGTATTATATAGTTGATATTAGAAGGACTTATAAAAGAGTTAAAAAGATATGATTATGAGTGATTTTGTAAAGACTAAAATCACTCATTTCAACTTGTCTCAAATCACTTTTCTTCTAAAATTGTGTTTGTTCTATTTCTAAATTTCTTCTTTACATCTAATAATCTTAACTTCCATGCATATGCATGTAATTGTTTTTAATCTAAATTTCTTTTAGTAATTGGATGATTCTACAACTAACATTAAAAACAAAAAAACATTAGTCCTTGAAAAAATTATTTCAATTGATCATACATGGAACAAAACTATTTCTACAAGTATCAAACCAAATATTTACTAGAAAAAAAAATACTTAGTTAAAAGTATAGAACCAAATATTAAAATGATTCTCGAACAAATCTTGATATTAGCGTTCAATTTTTTTTACATAGAAATAAACTTAAAACACAAAAGATATGGAAATTTCTTTTTTGTTATTATTATTATTATTTATCTTTAATTAAAATTTGTTAAAGAGTTGAGTATGATTAGTTTGTATGCAGATTTATTGGTTTCATAGGAAGGGAAGGGGTAATGCCTACTTCATATTTCCAAAAGCTATCTCAATAGCTTGGATCAATGAATGTCAGTACTGGAGATGGATATCTATCGACATCTTTGGGTATACTTATTTTTTCTAACCGCATTAAATATCGTCTTCGTTTCTATATTTTGAAATATCAATTTTCTAATATAACAAAATGTCACAATTTACAACATTCATTTAATTTGCTATATATTTATTTGTAAGTTAGTTAACTTAGTTGATTATATTTGAAAGCAGGTTATTAGTTCTTTCTCCTTTCAATAAGTTTTAATCCATATTGTTAGTTTATGGTTGATTTTACATAAATAAATAAACTCTTTGTTATGTATAAACAATATTAATTTTCCCATAAATTTAGAAAACACATATTCATGTAAGCTACATTTAAGATTTATTATGGAAAGTAGTTTATTAAAATTAAACAAAATTTAAATTACGAGAACAACATTTGAATTGGTTTTCTTTTTCTTTTTCTTTTTTTATGTGTTGAAAAATGAAATGATGAATTATGGAAGGTATATACTGAGATAAAAAAAATGAAAAAATAAAACACAAAAGAAGAGATAGATTAGGTTTGAATGAATGAACTTTAATCTTCATTATTTATTTACTAATCAAAATTAAAAGGGTTGTTTGGATGTAATTTTGTGAAATGGTTTTACCGAACAAAGTAGATTAAATTAGTCTCTATGATTTGATAAATGTTTCATTAATGAAACTACTTTCTAAAAATTGACAAGATTAAATTGAACTTAACTTTCTGTGAAACCATAGAGATGAATACGAGGTTTAAAAAATATAATAAGTAGTTTTTTTTAAGTAGTCGGTCATTATTTTAAAAGTTGTTTATTTGTTGATGATGGTGTAAAAAAACTTTGGCAGTAAGAAAATTGACGTGGCTGAGCTTGTAAAAGTTCGTTGGCTAGATGTACGTGTAAGGATCAAGGAATATATGCTATCAGCTGGCGTTACATATGAACTATTATGTCATCTTTTGTTAAAGTCGTGTGCATCGGGATGGCATGAACCTATTAACTTTGGACTCACCTTGCCAAATGGGACAAAATTTGTGAACAGTGAAAGCCTAGAATGTAAGCCACGAGATGTTTGGTTTACGATCAAGGTCGAGGAATTCAAGATTGGCGATAAACATGGTTGTTTGAATGCTAAGGAGTACGAGTTTAGCATGTATAACCACAATCAACATTGGAAGTCGGGATTGATCTTTAAAAGTTTTGAAATTCGACCAAAGCAACCATGTTGCAAAAGCTAAGAATATGAATCTCTAAGTTTGTCTCACTCCAACTACCCTACTTGTAGTTTGGTTTAAATAAATGAGAGAGGTATACTATACTCTAGTTTGTTTTATAATATAAACGACGGTATCAGAATGTAATAG

mRNA sequence

ATGGTTTCCTCAGAGCTTGAAGCAAGAGAGAACTCGGAAACAAAAGAGAAATATGGTCATTGCTTGAGTTATATTTTGCCAACAACTGGAGATCATGAGGTGCACTGGCCCTCCCCCTCTGAGCTCTATAAACAACTTTGTGATGGGACTTCCCTCAACAATGGATCTAAGATTTATTGGTTTCATAGGAAGGGAAGGGGTAATGCCTACTTCATATTTCCAAAAGCTATCTCAATAGCTTGGATCAATGAATGTCAGTACTGGAGATGGATATCTATCGACATCTTTGGTAAGAAAATTGACGTGGCTGAGCTTGTAAAAGTTCGTTGGCTAGATGTACGTGTAAGGATCAAGGAATATATGCTATCAGCTGGCGTTACATATGAACTATTATGTCATCTTTTGTTAAAGTCGTGTGCATCGGGATGGCATGAACCTATTAACTTTGGACTCACCTTGCCAAATGGGACAAAATTTGTGAACAGTGAAAGCCTAGAATGTAAGCCACGAGATGTTTGGTTTACGATCAAGGTCGAGGAATTCAAGATTGGCGATAAACATGGTTGTTTGAATGCTAAGGAGTACGAGTTTAGCATGTATAACCACAATCAACATTGGAAGTCGGGATTGATCTTTAAAAGTTTTGAAATTCGACCAAAGCAACCATGTTGCAAAAGCTAA

Coding sequence (CDS)

ATGGTTTCCTCAGAGCTTGAAGCAAGAGAGAACTCGGAAACAAAAGAGAAATATGGTCATTGCTTGAGTTATATTTTGCCAACAACTGGAGATCATGAGGTGCACTGGCCCTCCCCCTCTGAGCTCTATAAACAACTTTGTGATGGGACTTCCCTCAACAATGGATCTAAGATTTATTGGTTTCATAGGAAGGGAAGGGGTAATGCCTACTTCATATTTCCAAAAGCTATCTCAATAGCTTGGATCAATGAATGTCAGTACTGGAGATGGATATCTATCGACATCTTTGGTAAGAAAATTGACGTGGCTGAGCTTGTAAAAGTTCGTTGGCTAGATGTACGTGTAAGGATCAAGGAATATATGCTATCAGCTGGCGTTACATATGAACTATTATGTCATCTTTTGTTAAAGTCGTGTGCATCGGGATGGCATGAACCTATTAACTTTGGACTCACCTTGCCAAATGGGACAAAATTTGTGAACAGTGAAAGCCTAGAATGTAAGCCACGAGATGTTTGGTTTACGATCAAGGTCGAGGAATTCAAGATTGGCGATAAACATGGTTGTTTGAATGCTAAGGAGTACGAGTTTAGCATGTATAACCACAATCAACATTGGAAGTCGGGATTGATCTTTAAAAGTTTTGAAATTCGACCAAAGCAACCATGTTGCAAAAGCTAA
Homology
[Close]
BLAST of CSPI01G25330 vs. Swiss-Prot
Match: LEC_LUFAC (Lectin OS=Luffa acutangula PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.6e-32
Identity = 76/207 (36.71%), Postives = 112/207 (54.11%), Query Frame = 1 

Query: 17  KYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYWFHRKGRGNAYFIFPKA 76
           K GH L  IL          PS  +LY Q+  G  LNN +K YWF +    N + ++ + 
Sbjct: 9   KVGHNLEAILKGLDVDVYSVPSFIKLYDQVTAGIFLNNRTKRYWFDKNAESNCFMLYARD 68

Query: 77  ISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEYMLSAGVTYELLCHLLL 136
           + I W  + +YWRW      G  ++VAEL+ V WL++   I+  +LS G++YE    ++L
Sbjct: 69  LLITWSQDKRYWRWNPFQEHGNTLEVAELIDVCWLNIVGNIETSVLSPGISYEAAFEVML 128

Query: 137 KSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEEFKI--GDKHGCLNAKE 196
            + ASGW  P++  L +P+G++  +  +L+ KPR VWF I V  FKI  G+  G      
Sbjct: 129 TNSASGWRIPVDVKLKMPDGSEQESQVNLQDKPRGVWFFISVGHFKISVGETIG-----N 188

Query: 197 YEFSMYNHNQHWKSGLIFKSFEIRPKQ 222
            EFS+  H Q  K GL+ K   I+PKQ
Sbjct: 189 IEFSIVQH-QEAKRGLLVKGLVIQPKQ 209

[Close]
BLAST of CSPI01G25330 vs. Swiss-Prot
Match: P2A01_ARATH (Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana GN=PP2A1 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.9e-21
Identity = 62/188 (32.98%), Postives = 92/188 (48.94%), Query Frame = 1 

Query: 38  SPSELYKQLCDGTSLNNGSKIYWFHRKGRGNAYFIFPKAISIAWINECQYWRWISI-DIF 97
           S   L +QL  G  L    +I ++  +   N + +F K +SI W ++  YW W +  +  
Sbjct: 63  SSVNLSEQLRSGVFLKPKKQIKYWVDERNSNCFMLFAKNLSITWSDDVNYWTWFTEKESP 122

Query: 98  GKKIDVAELVKVRWLDVRVRIKEYMLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNG 157
            + ++   L  V WLD+  +     L+ G+ YE++  + L+  A GW  P+N  L LPNG
Sbjct: 123 NENVEAVGLKNVCWLDITGKFDTRNLTPGIVYEVVFKVKLEDPAYGWDTPVNLKLVLPNG 182

Query: 158 TKFVNSE--SLECKPRDVWFTIKVEEFKIGDKHGCLNAKEYEFSMYNHNQH-WKSGLIFK 217
            +    +  SL   PR  W  ++V EF + +K     A E  FSMY H    WK GL  K
Sbjct: 183 KEKPQEKKVSLRELPRYKWVDVRVGEF-VPEKSA---AGEITFSMYEHAAGVWKKGLSLK 242

Query: 218 SFEIRPKQ 222
              IRPKQ
Sbjct: 243 GVAIRPKQ 246

[Close]
BLAST of CSPI01G25330 vs. Swiss-Prot
Match: P2A04_ARATH (Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana GN=PP2A4 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.1e-19
Identity = 60/160 (37.50%), Postives = 81/160 (50.62%), Query Frame = 1 

Query: 67  GNAYFIFPKAISIAWINECQYWRWISI--DIFGKK-IDVAELVKVRWLDVRVRIKEYMLS 126
           G  + I+ + +SIAW ++ +YW W+ +  DI  +K +D A L  V WLDV  +     L+
Sbjct: 9   GQKHMIYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELT 68

Query: 127 AGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPR--DVWFTIKVEEF 186
              TYE++  + L+  ASGW+ P+N  LTLP+G K     S+  K      W  I   EF
Sbjct: 69  LETTYEVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEF 128

Query: 187 KIGDKHGCLNAKEYEFSMYNHNQ-HWKSGLIFKSFEIRPK 221
                    NA E  FSMY      WK GL  K  EIRPK
Sbjct: 129 VTSPD----NAGEISFSMYETKSCCWKRGLFVKCVEIRPK 164

[Close]
BLAST of CSPI01G25330 vs. Swiss-Prot
Match: P2A02_ARATH (Protein PHLOEM PROTEIN 2-LIKE A2 OS=Arabidopsis thaliana GN=PP2A2 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.2e-12
Identity = 44/123 (35.77%), Postives = 63/123 (51.22%), Query Frame = 1 

Query: 99  KIDVAELVKVRWLDVRVRIKEYMLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTK 158
           + +VA++ +V WL+V  + +   L+    YE++  + L   A GW   +NF L LP G  
Sbjct: 74  RTEVAKMERVAWLEVVGKFETEKLTPNSLYEVVFVVKLIDSAKGWDFRVNFKLVLPTGET 133

Query: 159 FVNSESLECKPRDVWFTIKVEEFKIGDKHGCLNAKEYEFSMYN-HNQHWKSGLIFKSFEI 218
               E++    R+ W  I   EF I  +H  L+ K  EFSM    +  WKSGLI K   I
Sbjct: 134 KERRENVNLLERNKWVEIPAGEFMISPEH--LSGK-IEFSMLEVKSDQWKSGLIVKGVAI 193

Query: 219 RPK 221
           RPK
Sbjct: 194 RPK 193

[Close]
BLAST of CSPI01G25330 vs. Swiss-Prot
Match: PP2B8_ARATH (Putative F-box protein PP2-B8 OS=Arabidopsis thaliana GN=PP2B8 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.4e-12
Identity = 59/207 (28.50%), Postives = 88/207 (42.51%), Query Frame = 1 

Query: 38  SPSELYKQLCDGTSL-NNGSKIYWFHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIF 97
           S  ELY  LCD + L ++G K  W  +        I    ++IAW N  Q WRWI  D  
Sbjct: 98  SKKELYFALCDKSVLIDDGKKSLWIEKANAKRCIMISAMNLAIAWGNSPQSWRWIP-DPQ 157

Query: 98  GKKIDVAELVKVRWLDVRVRIKEYMLSAGVTY--------------------ELLCHLLL 157
            +   VAEL++V   ++R RI   ++S    Y                    E++  ++ 
Sbjct: 158 ARFETVAELLEVCLFEIRGRINSRVISPKTRYSAYIVYKKLNICYGFENVAVEVVVGVVG 217

Query: 158 KSCASGWHEPINFGLTLPNGTKFVNSESLECKP---RDVWFTIKVEEFKIGDKHGCLNAK 217
           +         I F  T+    +  +      KP   +D W  IK+ EF   ++ G LN  
Sbjct: 218 QDLEESCRRYICFDETMDEQFRRRDRGKNLVKPERRKDGWMEIKIGEF--FNEGGLLNDD 277

Query: 218 EYEF-SMYNHNQHWKSGLIFKSFEIRP 220
           E E  ++    +HWK GLI +  EIRP
Sbjct: 278 EIEMVALEAKQRHWKRGLIIQGIEIRP 301

[Close]
BLAST of CSPI01G25330 vs. TrEMBL
Match: A0A0A0LY62_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G542500 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 2.5e-136
Identity = 225/226 (99.56%), Postives = 226/226 (100.00%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW
Sbjct: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           FHRKGRGNAYFIFPKAISIAWINECQYWRWISI+IFGKKIDVAELVKVRWLDVRVRIKEY
Sbjct: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFGKKIDVAELVKVRWLDVRVRIKEY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
           MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE
Sbjct: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCCKS 227
           FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCCKS
Sbjct: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCCKS 226

[Close]
BLAST of CSPI01G25330 vs. TrEMBL
Match: Q8LK97_CUCME (26 kDa phloem lectin OS=Cucumis melo GN=Lec26 PE=2 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 5.2e-94
Identity = 149/224 (66.52%), Postives = 187/224 (83.48%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MV  E EAREN + KEK+GHCLSYILP+TG+  VHWPS S+LY+QLC+G +LNNG+KIYW
Sbjct: 1   MVCLETEARENLQIKEKFGHCLSYILPSTGNPVVHWPSYSKLYQQLCEGITLNNGTKIYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           F +KG+GN YF+ PK++SIAWI++ +YW+WI +++ GKK++VAEL++V WLD R +IKEY
Sbjct: 61  FDKKGKGNGYFLLPKSLSIAWIDDRRYWKWIFVELSGKKLEVAELIRVSWLDARGKIKEY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
           MLS G+ YE+LCHLLLK  ASGWHEPINFGLT P+G  +VN ESLEC+PRDVWFT+KV E
Sbjct: 121 MLSPGIVYEVLCHLLLKPGASGWHEPINFGLTFPDGKTYVNQESLECRPRDVWFTVKVGE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCC 225
           FK+ D+HGC + KEYEFSMYNH  HWK+ +I K +EIRPKQ  C
Sbjct: 181 FKVDDRHGCDSTKEYEFSMYNHGGHWKTEMILKGYEIRPKQSSC 224

[Close]
BLAST of CSPI01G25330 vs. TrEMBL
Match: Q8LK13_CUCSA (26 kDa phloem protein OS=Cucumis sativus GN=PP2 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 2.5e-80
Identity = 131/224 (58.48%), Postives = 174/224 (77.68%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MV  E EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN G+K+YW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTINVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           F +KG+GN Y + PK+++I WI++CQYW+WI  +I+GKKIDVA+L+KV WLD+  ++K+Y
Sbjct: 61  FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYGKKIDVAQLIKVCWLDISGKLKQY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
            LS+GV YE+LCH+ +  CASGW EP+ F +TLP+G K    ESL+CKPRDVWFTIK+ E
Sbjct: 121 ALSSGVLYEVLCHVCVTPCASGWQEPVIFVITLPDGKKIETKESLQCKPRDVWFTIKIGE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCC 225
           FK+ DKH C + KEYEF +YNH+  WK+GL FK FEIRPKQ  C
Sbjct: 181 FKV-DKHDCNSDKEYEFRVYNHSSQWKTGLKFKGFEIRPKQSSC 223

[Close]
BLAST of CSPI01G25330 vs. TrEMBL
Match: A0A0A0LVN2_CUCSA (Phloem protein OS=Cucumis sativus GN=Csa_1G542510 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 2.5e-80
Identity = 131/224 (58.48%), Postives = 174/224 (77.68%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MV  E EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN G+K+YW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           F +KG+GN Y + PK+++I WI++CQYW+WI  +I+GKKIDVA+L+KV WLD+  ++K+Y
Sbjct: 61  FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYGKKIDVAQLIKVCWLDISGKLKQY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
            LS+GV YE+LCH+ +  CASGW EP+ F +TLP+G K    ESL+CKPRDVWFTIK+ E
Sbjct: 121 ALSSGVLYEVLCHVCVTPCASGWQEPVIFVITLPDGKKIETKESLQCKPRDVWFTIKIGE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCC 225
           FK+ DKH C + KEYEF +YNH+  WK+GL FK FEIRPKQ  C
Sbjct: 181 FKV-DKHDCNSDKEYEFRVYNHSSQWKTGLKFKGFEIRPKQSSC 223

[Close]
BLAST of CSPI01G25330 vs. TrEMBL
Match: W0ZFB6_CUCSA (Phloem lectin OS=Cucumis sativus GN=pp2 PE=2 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.4e-78
Identity = 130/224 (58.04%), Postives = 172/224 (76.79%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MV  E EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN G+K+YW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           F +KG+GN Y + PK+ +I WI++CQYW+WI  +I+GKKIDVA+L+KV WLD+  ++K+Y
Sbjct: 61  FDKKGKGNRYVLLPKSHTIIWIDDCQYWKWIPGEIYGKKIDVAQLIKVCWLDISGKLKQY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
            LS+GV YE+LCH+ +  CASGW EP+ F +TLP+G K    ESL+CKPRDVWFTIK+ E
Sbjct: 121 ALSSGVLYEVLCHVCVTPCASGWQEPVIFVITLPDGKKIETKESLQCKPRDVWFTIKIGE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCC 225
           FK+ DKH C + KEYEF +YNH+  WK+GL FK FEIR KQ  C
Sbjct: 181 FKV-DKHDCNSDKEYEFRVYNHSSQWKTGLKFKGFEIRLKQSSC 223

[Close]
BLAST of CSPI01G25330 vs. TAIR10
Match: AT4G19840.1 (AT4G19840.1 phloem protein 2-A1)

HSP 1 Score: 102.8 bits (255), Expect = 2.8e-22
Identity = 62/188 (32.98%), Postives = 92/188 (48.94%), Query Frame = 1 

Query: 38  SPSELYKQLCDGTSLNNGSKIYWFHRKGRGNAYFIFPKAISIAWINECQYWRWISI-DIF 97
           S   L +QL  G  L    +I ++  +   N + +F K +SI W ++  YW W +  +  
Sbjct: 63  SSVNLSEQLRSGVFLKPKKQIKYWVDERNSNCFMLFAKNLSITWSDDVNYWTWFTEKESP 122

Query: 98  GKKIDVAELVKVRWLDVRVRIKEYMLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNG 157
            + ++   L  V WLD+  +     L+ G+ YE++  + L+  A GW  P+N  L LPNG
Sbjct: 123 NENVEAVGLKNVCWLDITGKFDTRNLTPGIVYEVVFKVKLEDPAYGWDTPVNLKLVLPNG 182

Query: 158 TKFVNSE--SLECKPRDVWFTIKVEEFKIGDKHGCLNAKEYEFSMYNHNQH-WKSGLIFK 217
            +    +  SL   PR  W  ++V EF + +K     A E  FSMY H    WK GL  K
Sbjct: 183 KEKPQEKKVSLRELPRYKWVDVRVGEF-VPEKSA---AGEITFSMYEHAAGVWKKGLSLK 242

Query: 218 SFEIRPKQ 222
              IRPKQ
Sbjct: 243 GVAIRPKQ 246

[Close]
BLAST of CSPI01G25330 vs. TAIR10
Match: AT1G33920.1 (AT1G33920.1 phloem protein 2-A4)

HSP 1 Score: 97.4 bits (241), Expect = 1.2e-20
Identity = 60/160 (37.50%), Postives = 81/160 (50.62%), Query Frame = 1 

Query: 67  GNAYFIFPKAISIAWINECQYWRWISI--DIFGKK-IDVAELVKVRWLDVRVRIKEYMLS 126
           G  + I+ + +SIAW ++ +YW W+ +  DI  +K +D A L  V WLDV  +     L+
Sbjct: 9   GQKHMIYARDLSIAWSDKDEYWSWLPLRYDISSEKLVDAAVLEAVCWLDVNGKFDTRELT 68

Query: 127 AGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPR--DVWFTIKVEEF 186
              TYE++  + L+  ASGW+ P+N  LTLP+G K     S+  K      W  I   EF
Sbjct: 69  LETTYEVVYVVKLEDTASGWNIPVNLKLTLPDGKKRPQERSMCLKEHIGKRWIDISAGEF 128

Query: 187 KIGDKHGCLNAKEYEFSMYNHNQ-HWKSGLIFKSFEIRPK 221
                    NA E  FSMY      WK GL  K  EIRPK
Sbjct: 129 VTSPD----NAGEISFSMYETKSCCWKRGLFVKCVEIRPK 164

[Close]
BLAST of CSPI01G25330 vs. TAIR10
Match: AT2G02340.1 (AT2G02340.1 phloem protein 2-B8)

HSP 1 Score: 72.0 bits (175), Expect = 5.3e-13
Identity = 59/207 (28.50%), Postives = 88/207 (42.51%), Query Frame = 1 

Query: 38  SPSELYKQLCDGTSL-NNGSKIYWFHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIF 97
           S  ELY  LCD + L ++G K  W  +        I    ++IAW N  Q WRWI  D  
Sbjct: 98  SKKELYFALCDKSVLIDDGKKSLWIEKANAKRCIMISAMNLAIAWGNSPQSWRWIP-DPQ 157

Query: 98  GKKIDVAELVKVRWLDVRVRIKEYMLSAGVTY--------------------ELLCHLLL 157
            +   VAEL++V   ++R RI   ++S    Y                    E++  ++ 
Sbjct: 158 ARFETVAELLEVCLFEIRGRINSRVISPKTRYSAYIVYKKLNICYGFENVAVEVVVGVVG 217

Query: 158 KSCASGWHEPINFGLTLPNGTKFVNSESLECKP---RDVWFTIKVEEFKIGDKHGCLNAK 217
           +         I F  T+    +  +      KP   +D W  IK+ EF   ++ G LN  
Sbjct: 218 QDLEESCRRYICFDETMDEQFRRRDRGKNLVKPERRKDGWMEIKIGEF--FNEGGLLNDD 277

Query: 218 EYEF-SMYNHNQHWKSGLIFKSFEIRP 220
           E E  ++    +HWK GLI +  EIRP
Sbjct: 278 EIEMVALEAKQRHWKRGLIIQGIEIRP 301

[Close]
BLAST of CSPI01G25330 vs. TAIR10
Match: AT1G80110.1 (AT1G80110.1 phloem protein 2-B11)

HSP 1 Score: 66.6 bits (161), Expect = 2.2e-11
Identity = 60/231 (25.97%), Postives = 96/231 (41.56%), Query Frame = 1 

Query: 9   RENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKI-YWFHRKGRG 68
           R  + + + + H L    P         P+  +L+  L D   L NG+ + +   RK   
Sbjct: 32  RSAAGSDDVWNHFLPADFPAGFAAPAGLPTRKQLFFSLVDNPLLINGTLLSFSLERKSGN 91

Query: 69  NAYFIFPKAISIAWINECQYWRWISIDI--FGKKIDVAELVKVRWLDVRVRIKEYMLSAG 128
             Y +  +A++I W +E +YW WIS+    FG   +VAEL+ V WL++  +I   +LS  
Sbjct: 92  KCYMMAARALNIVWGHEQRYWHWISLPNTRFG---EVAELIMVWWLEITGKINITLLSDD 151

Query: 129 VTYELLCHLLLKSCASGWHEPINFGLTLPN--GTKFVNSESL--------------ECKP 188
             Y             G+ +P+   L L +   T  V   S+                  
Sbjct: 152 TLYAAYFVFKWNHSPYGFRQPVETSLVLADTESTDNVVQPSMISLMQDSGGEEGQSPVLR 211

Query: 189 RDVWFTIKVEEFKIGDKHGCLNAKEYEFSM-YNHNQHWKSGLIFKSFEIRP 220
           RD W+ +++ +F    + G L   E E S+      + K GLI    EIRP
Sbjct: 212 RDGWYEVELGQF--FKRRGDLG--EIEMSLKETKGPYEKKGLIVYGIEIRP 255

[Close]
BLAST of CSPI01G25330 vs. TAIR10
Match: AT5G45090.1 (AT5G45090.1 phloem protein 2-A7)

HSP 1 Score: 62.0 bits (149), Expect = 5.5e-10
Identity = 44/141 (31.21%), Postives = 66/141 (46.81%), Query Frame = 1 

Query: 88  WRWISI--DIFGKKIDVAELVKVRWLDVRVRIKEYMLSAGVTYELLCHLLLKSCASGWHE 147
           W W SI        I+VA L +V WL +        L+ G  YE++  + L   +SGW +
Sbjct: 195 WTWSSIYETPNDAAIEVAMLNEVHWLHMSGNFHTRNLTPGTKYEVVFLVSLDDTSSGWEQ 254

Query: 148 PINFGLTL--PNGTKFVNSE--SLECKPRDVWFTIKVEEFKIGDKHGCLNAKEYEFSMYN 207
           P+N  L +  P+GT+ +     SLEC   + W  I+        ++    A +  F+MY 
Sbjct: 255 PVNLNLKVINPDGTESLQERETSLECHIGENWVDIQAGVLVAPPRNA---AAKMTFTMYQ 314

Query: 208 H-NQHWKSGLIFKSFEIRPKQ 222
           +     KSGL+ K   IRP Q
Sbjct: 315 YVTSDRKSGLVVKGVAIRPMQ 332

[Close]
BLAST of CSPI01G25330 vs. NCBI nr
Match: gi|449435554|ref|XP_004135560.1| (PREDICTED: uncharacterized protein PHLOEM PROTEIN 2-LIKE A4-like [Cucumis sativus])

HSP 1 Score: 492.7 bits (1267), Expect = 3.5e-136
Identity = 225/226 (99.56%), Postives = 226/226 (100.00%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW
Sbjct: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           FHRKGRGNAYFIFPKAISIAWINECQYWRWISI+IFGKKIDVAELVKVRWLDVRVRIKEY
Sbjct: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIEIFGKKIDVAELVKVRWLDVRVRIKEY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
           MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE
Sbjct: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCCKS 227
           FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCCKS
Sbjct: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCCKS 226

[Close]
BLAST of CSPI01G25330 vs. NCBI nr
Match: gi|661902909|ref|NP_001284457.1| (protein PHLOEM PROTEIN 2-LIKE A1-like [Cucumis melo])

HSP 1 Score: 352.1 bits (902), Expect = 7.5e-94
Identity = 149/224 (66.52%), Postives = 187/224 (83.48%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MV  E EAREN + KEK+GHCLSYILP+TG+  VHWPS S+LY+QLC+G +LNNG+KIYW
Sbjct: 1   MVCLETEARENLQIKEKFGHCLSYILPSTGNPVVHWPSYSKLYQQLCEGITLNNGTKIYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           F +KG+GN YF+ PK++SIAWI++ +YW+WI +++ GKK++VAEL++V WLD R +IKEY
Sbjct: 61  FDKKGKGNGYFLLPKSLSIAWIDDRRYWKWIFVELSGKKLEVAELIRVSWLDARGKIKEY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
           MLS G+ YE+LCHLLLK  ASGWHEPINFGLT P+G  +VN ESLEC+PRDVWFT+KV E
Sbjct: 121 MLSPGIVYEVLCHLLLKPGASGWHEPINFGLTFPDGKTYVNQESLECRPRDVWFTVKVGE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCC 225
           FK+ D+HGC + KEYEFSMYNH  HWK+ +I K +EIRPKQ  C
Sbjct: 181 FKVDDRHGCDSTKEYEFSMYNHGGHWKTEMILKGYEIRPKQSSC 224

[Close]
BLAST of CSPI01G25330 vs. NCBI nr
Match: gi|449435552|ref|XP_004135559.1| (PREDICTED: uncharacterized protein PHLOEM PROTEIN 2-LIKE A4-like [Cucumis sativus])

HSP 1 Score: 306.6 bits (784), Expect = 3.6e-80
Identity = 131/224 (58.48%), Postives = 174/224 (77.68%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MV  E EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN G+K+YW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           F +KG+GN Y + PK+++I WI++CQYW+WI  +I+GKKIDVA+L+KV WLD+  ++K+Y
Sbjct: 61  FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYGKKIDVAQLIKVCWLDISGKLKQY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
            LS+GV YE+LCH+ +  CASGW EP+ F +TLP+G K    ESL+CKPRDVWFTIK+ E
Sbjct: 121 ALSSGVLYEVLCHVCVTPCASGWQEPVIFVITLPDGKKIETKESLQCKPRDVWFTIKIGE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCC 225
           FK+ DKH C + KEYEF +YNH+  WK+GL FK FEIRPKQ  C
Sbjct: 181 FKV-DKHDCNSDKEYEFRVYNHSSQWKTGLKFKGFEIRPKQSSC 223

[Close]
BLAST of CSPI01G25330 vs. NCBI nr
Match: gi|22023939|gb|AAM89256.1|AF527536_1 (26 kDa phloem protein [Cucumis sativus])

HSP 1 Score: 306.6 bits (784), Expect = 3.6e-80
Identity = 131/224 (58.48%), Postives = 174/224 (77.68%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MV  E EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN G+K+YW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTINVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           F +KG+GN Y + PK+++I WI++CQYW+WI  +I+GKKIDVA+L+KV WLD+  ++K+Y
Sbjct: 61  FDKKGKGNRYVLLPKSLTIIWIDDCQYWKWIPGEIYGKKIDVAQLIKVCWLDISGKLKQY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
            LS+GV YE+LCH+ +  CASGW EP+ F +TLP+G K    ESL+CKPRDVWFTIK+ E
Sbjct: 121 ALSSGVLYEVLCHVCVTPCASGWQEPVIFVITLPDGKKIETKESLQCKPRDVWFTIKIGE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCC 225
           FK+ DKH C + KEYEF +YNH+  WK+GL FK FEIRPKQ  C
Sbjct: 181 FKV-DKHDCNSDKEYEFRVYNHSSQWKTGLKFKGFEIRPKQSSC 223

[Close]
BLAST of CSPI01G25330 vs. NCBI nr
Match: gi|571272670|emb|CDK12646.1| (phloem lectin [Cucumis sativus])

HSP 1 Score: 300.8 bits (769), Expect = 2.0e-78
Identity = 130/224 (58.04%), Postives = 172/224 (76.79%), Query Frame = 1 

Query: 1   MVSSELEARENSETKEKYGHCLSYILPTTGDHEVHWPSPSELYKQLCDGTSLNNGSKIYW 60
           MV  E EARE+ + +E YGHCL+YILP T +  VHWP+ ++LY+QL  G +LN G+K+YW
Sbjct: 1   MVCIETEARESLQIQESYGHCLTYILPKTLNVTVHWPTYAKLYQQLIAGITLNKGTKLYW 60

Query: 61  FHRKGRGNAYFIFPKAISIAWINECQYWRWISIDIFGKKIDVAELVKVRWLDVRVRIKEY 120
           F +KG+GN Y + PK+ +I WI++CQYW+WI  +I+GKKIDVA+L+KV WLD+  ++K+Y
Sbjct: 61  FDKKGKGNRYVLLPKSHTIIWIDDCQYWKWIPGEIYGKKIDVAQLIKVCWLDISGKLKQY 120

Query: 121 MLSAGVTYELLCHLLLKSCASGWHEPINFGLTLPNGTKFVNSESLECKPRDVWFTIKVEE 180
            LS+GV YE+LCH+ +  CASGW EP+ F +TLP+G K    ESL+CKPRDVWFTIK+ E
Sbjct: 121 ALSSGVLYEVLCHVCVTPCASGWQEPVIFVITLPDGKKIETKESLQCKPRDVWFTIKIGE 180

Query: 181 FKIGDKHGCLNAKEYEFSMYNHNQHWKSGLIFKSFEIRPKQPCC 225
           FK+ DKH C + KEYEF +YNH+  WK+GL FK FEIR KQ  C
Sbjct: 181 FKV-DKHDCNSDKEYEFRVYNHSSQWKTGLKFKGFEIRLKQSSC 223

The following BLAST results are available for this feature:
BLAST of CSPI01G25330 vs. Swiss-Prot
Match NameE-valueIdentityDescription
LEC_LUFAC1.6e-3236.71Lectin OS=Luffa acutangula PE=1 SV=1[more]
P2A01_ARATH4.9e-2132.98Protein PHLOEM PROTEIN 2-LIKE A1 OS=Arabidopsis thaliana GN=PP2A1 PE=2 SV=1[more]
P2A04_ARATH2.1e-1937.50Uncharacterized protein PHLOEM PROTEIN 2-LIKE A4 OS=Arabidopsis thaliana GN=PP2A... [more]
P2A02_ARATH7.2e-1235.77Protein PHLOEM PROTEIN 2-LIKE A2 OS=Arabidopsis thaliana GN=PP2A2 PE=2 SV=1[more]
PP2B8_ARATH9.4e-1228.50Putative F-box protein PP2-B8 OS=Arabidopsis thaliana GN=PP2B8 PE=3 SV=1[more]
BLAST of CSPI01G25330 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LY62_CUCSA2.5e-13699.56Uncharacterized protein OS=Cucumis sativus GN=Csa_1G542500 PE=4 SV=1[more]
Q8LK97_CUCME5.2e-9466.5226 kDa phloem lectin OS=Cucumis melo GN=Lec26 PE=2 SV=1[more]
Q8LK13_CUCSA2.5e-8058.4826 kDa phloem protein OS=Cucumis sativus GN=PP2 PE=2 SV=1[more]
A0A0A0LVN2_CUCSA2.5e-8058.48Phloem protein OS=Cucumis sativus GN=Csa_1G542510 PE=4 SV=1[more]
W0ZFB6_CUCSA1.4e-7858.04Phloem lectin OS=Cucumis sativus GN=pp2 PE=2 SV=1[more]
BLAST of CSPI01G25330 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G19840.12.8e-2232.98 phloem protein 2-A1[more]
AT1G33920.11.2e-2037.50 phloem protein 2-A4[more]
AT2G02340.15.3e-1328.50 phloem protein 2-B8[more]
AT1G80110.12.2e-1125.97 phloem protein 2-B11[more]
AT5G45090.15.5e-1031.21 phloem protein 2-A7[more]
BLAST of CSPI01G25330 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449435554|ref|XP_004135560.1|3.5e-13699.56PREDICTED: uncharacterized protein PHLOEM PROTEIN 2-LIKE A4-like [Cucumis sativu... [more]
gi|661902909|ref|NP_001284457.1|7.5e-9466.52protein PHLOEM PROTEIN 2-LIKE A1-like [Cucumis melo][more]
gi|449435552|ref|XP_004135559.1|3.6e-8058.48PREDICTED: uncharacterized protein PHLOEM PROTEIN 2-LIKE A4-like [Cucumis sativu... [more]
gi|22023939|gb|AAM89256.1|AF527536_13.6e-8058.4826 kDa phloem protein [Cucumis sativus][more]
gi|571272670|emb|CDK12646.1|2.0e-7858.04phloem lectin [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR025886PP2-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0030246 carbohydrate binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G25330.1CSPI01G25330.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025886Phloem protein 2-likePFAMPF14299PP2coord: 69..219
score: 2.0
NoneNo IPR availablePANTHERPTHR32278FAMILY NOT NAMEDcoord: 33..221
score: 1.9
NoneNo IPR availablePANTHERPTHR32278:SF3SUBFAMILY NOT NAMEDcoord: 33..221
score: 1.9