CSPI01G25250 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G25250
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	Chr1 : 20709717 .. 20714731 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAAATGGGGTGATAAGAAGCGACATTCTCTTGAGTTTCGAGCAGGAGATCAAGTCCTCATCAAGCTGAAAACAGATCAAATTCGGTTTAGAGGGCGCAAAGATCAGCACCTTGTCAGAAAATATGAGGGGTTTGGGGAAGTCCTCAAGAAGATAGGAAATGCATAGTATAGGGTGTTGTTGCCTACATGGATGAAAATTCACCCAGTAATTCATGTGAGCAACTTAAAACCCTACCATCAAGACCCCGACGACAAGCAGCACGACATATTGCTCGACCATGTATCAACCTGAAGCAGAAAGAAGATAAAGAAGTTGAAGAGATCCTTGCAGATCGAGTAAGGAAGGGTAGAGGCCCACAAGGAGAATCCACGAAATCCTGGTTAGATGGAAAAACCTCCCCGTGGAAGAAACGGGTTGGGAACGTGTTGAAGACATTAGAGCGTGGAAGCAGAAGATCGAAGAGTTCTAGCTTCGTCAGTCGATAGGGACGTCAGCTGTTTAAGTGGGGGAGAATGTTATGAGCATGCTTGTCCAAGGCGTTGTTTGTCTATGACCACATGCACAAACATCCACTCCCCACCTTATGTTTATACTTTTGTACTTAGTTTAGCTTATTTTCTTTAATTTTTGTCGCTTACTTGTAAGTGACGTTTCAAACTTTTCATATGTAAGCCTTTCGTATTTGAAAATGTTAAAAATGTACTATAAGGGAAACTCACTAGTCAATTCCCCGACTAAGCCTTGTTACTTTGGAAACTGAACTTTCTTACAATTGTCAGTCTTCAATGCTTTCTTTAATAATGTTTTCAATGCTTTTCTTTCTCTCACGTTTTATAAAACCATTTTCTCTAAAAACATGAATGGAGGCTACATCATATTGCAATGTTGCCGTGACTTTTGGATTTAGTACGGCTAACTTATTCATTGAGATAGCACGTTTTATAAAATATCTTCCTCCCAAAATTCTTTTTGTCTTCTGCTAAAACCCACAACTCATCACCGTCACGACTCCGACTCCAACTGTCAACACTTCGCAGAACCTGCCGGAAACCCAAGAAAACTACACTTTTCAATCAAAACCAAGGATATCTGAATCCTCACTTCCTTCATCACAATGATAATACAAGTCTGGTGTTAGCAACGGAGCAATTGACAGAAGAGAATTATGTTTCTTGGACTCAAGCGATGACCATTGGTCTTTCAGTGAAGAACAAGATTGGGTTCGTCGACGAGACTATTACCAAACGAACCGGTGATCTCCTTCCGGCTTGGATTAGAAATAACATCGTTATTTCTTGGATCCTAAACTCAGTCTCTAAACCTGTCTCAGCAAGTATTTTGTTTTCAGGTTCAACCAGAGCAATATGGATTGACCTCAAAGAAAGATTTCAGAAGAAGAACGTGCCAAGGATTTTTCAGTTGAAGCGATCCCTTGCAACCTTGGAACAAAACCAAGATACCATTGGTGTGTATTATACTAAATTCAATACTCTCATTGATGAACTGAACACATATAGACCAGGGTGCACTTGCGGAGCTTGCAGTTGTGACATCGTGCGAGAAATGACAGATTTCCTCCAAATGTAATATCTCATGGACTTATGGGATTGAATGAGAACATCTCTCAAGCCCGGGCTCAACTTCTCCTCATGGATCCTCTTCCATCAACAAGCCGAGCTTTCTCTCTTCTTCTTCAAGAGGAACAACAAAGATCAATTGGATCTTTTTCTTCTACAGCACCAACGATGGCCTTTGCAGTATCTTCTAACTCATCCAAGAATGAATCCACCAATCGACAAAGGAGAGAAAAGACTATATGCACCCACTGCAACATTTCTGAACACATGATAGATTAGTGTTACAAGCTCCATGGATACCCTCCTGGATATAAGACCAAGCAACAACAGCAGTGAACCAATAATGTTGTTAATGCAGTAGCAACTCAGAACAATGAAAGTCGTTCTCAAGGTACCACACAGAGTAATCAGATATTGAATAATACCAGCACTGCAGAAGCTTTGATCCAGTGTCAAAACCTCCTCAACCAGCTTCAGTCTCAAATGAATGCTTCCAACCAACCAACTACCTCACATAAAGCAGGTACTTCTTATTCATTTCCCCTGTGGATAATTGATCCTGGAGCATCCACTCACATTTCTTGTTGCAAGTCCCATTTTGCATCCATTCAACCATGCTCAACATCCATCCGTTTACCTAATAAACAAGTTTTTGAAGGTAAAAGTGCTAGCACTATCAAATTATCTGAATCTATAGAGCTGAAGAATGTGTTATATATTCCTGAATTTTCATTCAACCTCAGTGAGCGCATTAACAAGAGATCTACTCGTCGATGTTAGTTTCTCTACTAATGGTTGTGTAACTCAGGACAAGTTCACTTTGAAGAAGATTGGCAATGCTGAACTTTTATATGGTCTATATGTCTTCAAATTGGGAAACACTCTTGATCTGCAGTCTACCATATGTGCTGTAAGCCATGATAATACTTTTTTGTGGCATCAAAGGCTTGGTCACCCTTCTGTTGATGTTTTGAAATCTTTGCAAGACGTGTTGCAATTGAAGTCTTTTAATTTTCATTCTTGTACTACTTGTCCCTTAGCAAAGCAAAGAAGACTTTCTTTTTCTTCAAATAATCGAGTGTCTCCAAATCCATGTACATACATGTATAAACTTTCCTCATTGTTCATAAATAAAGTGAGTGAAATATTTCTTCTATTCTCTGTTAATATCTGGCTTCTCATCCTTTCTTAATTTTTCTTGCTGTATACAGTTGCAGAATTGGTCTGGATGTCCACCTAATATGCATGATATAAGTGGAAATGCACACTTCATAAATAAATGTGATAATGGAATTGTCATTCATCGTAATAGGGATCCTGAAAGTGGTCCTATTGATCTCGTACAGGTACAATTGCAGATATATTATAAAAACAATATGTTTCTTTCTTTTCTGTGTACATTAGTTTTCTTTGTATCCTTGCTACTTGTAGGTATGTGTACGAAAAGTGAGAAATAAGGTTGCAGGAACAATTGGGGAAGCTTATTTGGCATATAATAGGTATGTGAAGATCGTTTTCCAAAGTTTTTCAGGAATTCACATCTTGGTTCTGACTTCTTGTGGTGATGAATTGTCCTATATGCTTTCTTGAAATTAAGGTTGTTGAACAATGAACATTATATATTCCTTAATGATAAGAGTTTTATGAAAAAACATTAGGTAGCAACATTTTCCCAGTGTTTTGAACAATCTTTGAAAGCAAACCGTTTCTTTTATTTTGGAGAACTACTCTGGTTATCCGTTATGGTTTTGCACTGTGTACTTTGAAGTTTGGCCAATTTCTTGTTGAAGGATTTGTGGATGAGATTCATTGCTTAAGCCTTGGTGTTTAAAATATTCTCTTGAAGTTTTGGTCAATCATCATTCTAGTTAACAAGGACTTCATACCGATACACATGGCACACTGAAATCAACAAACATAAAATCATTCAGAAAAAGTACCTCGCACTAGCCCTCTCTCCTTGTTGGTGCATTATGTGCAAGATGTCAAGAAATGGGTAGAGTTTTTCCTCACGTTCTCGTTTGACTGTATTGGCCCACAATCTGAAAAGGACAATCCTTTAATTTAATTGGAGCGTAGCTCCCCTAGCCATTCTTTCAAAAAAGTTCGGGAGCATCCTTGGCTTCACATCAATTAAGGATTCTTTTGGTTACCATGGATCAAAATTGTTTGATGAATTAAGGAACAACCCACCCTTCAGAAATTGTTTGATGAGATATGTTGCACATACTAAAACAGTTTCTTAACCAAAATCTGAGGGGTCTTTGCCTTGGGAAATTTTGCCAATAAATGCGTAGTCTGTCGGTTACAGTGATTCTTTGTGGACAGTGGCCTTAAAGCGTTGCTGCGAATAGTAATCTGTTAGTCCTTAAGGGTTGGATAGTCGAGTTTACAACTTACACCAGTCAAATCTCTTGGATTTCTATCTTCAAAACTAGTCTTCCTTCTAAGATTGATTGTTGTGTTTTAAAGTTAGACTTTGAGACGACTCGTGGACAAAAGCCACCCCTTATGCCATCAACAAAACATGTGATTTCTTATAGAACTCTATAATTCATGTAGTCACTAGTCAGAATGCTAAGCACTCATGAGAGTGATTAAGCAACAGGGAATAACCCTCTAACTTTTAGGAATGCAACTGGATCCCCCTTTTTTATTGAATTATTGAGTTCGGTTGCAACTTTAGCCAATCATTTGCTGCAAGGGTTGAATGACTGAATGCTGATCCCTCGATAGTCAAAATAACTGACAATGATGCCATCTGGAGGCTTTCAGCTACCTAATAGGTTCTTGTAATACGTATCTGTGTGGTTTCTGTTTGCGGAAACTCAACCTTGATGGAGTTCAAAGAACAAAACATAAATTCTAACTCTGCCTAGATGTTTGGTTTGTGAGAAAGGGGGAAAACACAGACCACTTTTGCTTTCATTATCAGCCCAATGATTGCAAGGTACATGATTGACAATCATTACCTGTGGGCCTTTTGTGCTATTCTAGTGTTTTCTGGAGTGAGTGAGTCATCGAGTGGCATGCAAATTGCAATACGCTCCTTCTATTCTCTGTTTATCAGCATGTCACTGTATCAATGTACTTTTATTTGAAACCCTTTGTTCTCTGTATATCCGCTATTAAAAGGTCAATAATGGCTGTTATGCTCATCAGGGTAACCGGAGAATTCTTCGATGCTGCTGGGGATATGAAACTTAAGAAACCATCATCTTGAGAGGAGGTATGGTGTTGAAGGCCTTCACAAGTATTGTTTAAGGTCATTCATCCACTGCAATACTGTGAATTGTCGATAAATGTCGACTGCTAATTCATTCGTACAATTCACATTTATTTAGCAATGTCATTTTTGTTTTGGGTGATCCATAGCAAGAAGAAATTATTAAGTTGATGGATGTGTATAATTATTAAGTTGATGGATGTGTA

mRNA sequence

ATGAAAAAATGGGGTGATAAGAAGCGACATTCTCTTGAGTTTCGAGCAGGAGATCAAGTCCTCATCAAGCTGAAAACAGATCAAATTCGGTTTAGAGGGCGCAAAGATCAGCACCTTGTCAGAAAATATGAGGGGTTTGGGGAAGGTGTTGTTGCCTACATGGATGAAAATTCACCCAGTAATTCATGTGAGCAACTTAAAACCCTACCATCAAGACCCCGACGACAAGCAGCACGACATATTGCTCGACCATGTATCAACCTGAAGCAGAAAGAAGATAAAGAAGTTGAAGAGATCCTTGCAGATCGAAGCGTGGAAGCAGAAGATCGAAGAGTTCTAGCTTCGTCAGTCGATAGGGACGTCAGCTGTTTAAGTGGGGGAGAATGTTATGAGCATGCTTGTCCAAGGCGTTGTTTAACCTGCCGGAAACCCAAGAAAACTACACTTTTCAATCAAAACCAAGGATATCTGAATCCTCACTTCCTTCATCACAATGATAATACAAGTCTGGTGTTAGCAACGGAGCAATTGACAGAAGAGAATTATGTTTCTTGGACTCAAGCGATGACCATTGGTCTTTCAGTGAAGAACAAGATTGGGTTCGTCGACGAGACTATTACCAAACGAACCGGTGATCTCCTTCCGGCTTGGATTAGAAATAACATCGTTATTTCTTGGATCCTAAACTCAGTCTCTAAACCTGTCTCAGCAAGTATTTTGTTTTCAGGTTCAACCAGAGCAATATGGATTGACCTCAAAGAAAGATTTCAGAAGAAGAACGTGCCAAGGATTTTTCAGTTGAAGCGATCCCTTGCAACCTTGGAACAAAACCAAGATACCATTGGTGTAAATGACAGATTTCCTCCAAATGTAATATCTCATGGACTTATGGGATTGAATGAGAACATCTCTCAAGCCCGGGCTCAACTTCTCCTCATGGATCCTCTTCCATCAACAAGCCGAGCTTTCTCTCTTCTTCTTCAAGAGGAACAACAAAGATCAATTGGATCTTTTTCTTCTACAGCACCAACGATGGCCTTTGCAATTAGTGTTACAAGCTCCATGGATACCCTCCTGGATATAAGACCAAGCAACAACAGCATAGCAACTCAGAACAATGAAAGTCGTTCTCAAGGTACCACACAGAGTAATCAGATATTGAATAATACCAGCACTGCAGAAGCTTTGATCCAGTGTCAAAACCTCCTCAACCAGCTTCAGTCTCAAATGAATGCTTCCAACCAACCAACTACCTCACATAAAGCAGGTACTTCTTATTCATTTCCCCTGTGGATAATTGATCCTGGAGCATCCACTCACATTTCTTGTTGCAAGTCCCATTTTGCATCCATTCAACCATGCTCAACATCCATCCGTTTACCTAATAAACAAGTTTTTGAAGTGAGCGCATTAACAAGAGATCTACTCGTCGATGTTAGTTTCTCTACTAATGGTTGTGTAACTCAGGACAAGTTCACTTTGAAGAAGATTGGCAATGCTGAACTTTTATATGGTCTATATGTCTTCAAATTGGGAAACACTCTTGATCTGCAGTCTACCATATGTGCTTTGCAGAATTGGTCTGGATGTCCACCTAATATGCATGATATAAGTGGAAATGCACACTTCATAAATAAATGTGATAATGGAATTGTCATTCATCGTAATAGGGATCCTGAAAGTGGTCCTATTGATCTCGTACAGGTATGTGTACGAAAAGTGAGAAATAAGGTTGCAGGAACAATTGGGGAAGCTTATTTGGCATATAATAGGGTAACCGGAGAATTCTTCGATGCTGCTGGGGATATGAAACTTAAGAAACCATCATCTTGA

Coding sequence (CDS)

ATGAAAAAATGGGGTGATAAGAAGCGACATTCTCTTGAGTTTCGAGCAGGAGATCAAGTCCTCATCAAGCTGAAAACAGATCAAATTCGGTTTAGAGGGCGCAAAGATCAGCACCTTGTCAGAAAATATGAGGGGTTTGGGGAAGGTGTTGTTGCCTACATGGATGAAAATTCACCCAGTAATTCATGTGAGCAACTTAAAACCCTACCATCAAGACCCCGACGACAAGCAGCACGACATATTGCTCGACCATGTATCAACCTGAAGCAGAAAGAAGATAAAGAAGTTGAAGAGATCCTTGCAGATCGAAGCGTGGAAGCAGAAGATCGAAGAGTTCTAGCTTCGTCAGTCGATAGGGACGTCAGCTGTTTAAGTGGGGGAGAATGTTATGAGCATGCTTGTCCAAGGCGTTGTTTAACCTGCCGGAAACCCAAGAAAACTACACTTTTCAATCAAAACCAAGGATATCTGAATCCTCACTTCCTTCATCACAATGATAATACAAGTCTGGTGTTAGCAACGGAGCAATTGACAGAAGAGAATTATGTTTCTTGGACTCAAGCGATGACCATTGGTCTTTCAGTGAAGAACAAGATTGGGTTCGTCGACGAGACTATTACCAAACGAACCGGTGATCTCCTTCCGGCTTGGATTAGAAATAACATCGTTATTTCTTGGATCCTAAACTCAGTCTCTAAACCTGTCTCAGCAAGTATTTTGTTTTCAGGTTCAACCAGAGCAATATGGATTGACCTCAAAGAAAGATTTCAGAAGAAGAACGTGCCAAGGATTTTTCAGTTGAAGCGATCCCTTGCAACCTTGGAACAAAACCAAGATACCATTGGTGTAAATGACAGATTTCCTCCAAATGTAATATCTCATGGACTTATGGGATTGAATGAGAACATCTCTCAAGCCCGGGCTCAACTTCTCCTCATGGATCCTCTTCCATCAACAAGCCGAGCTTTCTCTCTTCTTCTTCAAGAGGAACAACAAAGATCAATTGGATCTTTTTCTTCTACAGCACCAACGATGGCCTTTGCAATTAGTGTTACAAGCTCCATGGATACCCTCCTGGATATAAGACCAAGCAACAACAGCATAGCAACTCAGAACAATGAAAGTCGTTCTCAAGGTACCACACAGAGTAATCAGATATTGAATAATACCAGCACTGCAGAAGCTTTGATCCAGTGTCAAAACCTCCTCAACCAGCTTCAGTCTCAAATGAATGCTTCCAACCAACCAACTACCTCACATAAAGCAGGTACTTCTTATTCATTTCCCCTGTGGATAATTGATCCTGGAGCATCCACTCACATTTCTTGTTGCAAGTCCCATTTTGCATCCATTCAACCATGCTCAACATCCATCCGTTTACCTAATAAACAAGTTTTTGAAGTGAGCGCATTAACAAGAGATCTACTCGTCGATGTTAGTTTCTCTACTAATGGTTGTGTAACTCAGGACAAGTTCACTTTGAAGAAGATTGGCAATGCTGAACTTTTATATGGTCTATATGTCTTCAAATTGGGAAACACTCTTGATCTGCAGTCTACCATATGTGCTTTGCAGAATTGGTCTGGATGTCCACCTAATATGCATGATATAAGTGGAAATGCACACTTCATAAATAAATGTGATAATGGAATTGTCATTCATCGTAATAGGGATCCTGAAAGTGGTCCTATTGATCTCGTACAGGTATGTGTACGAAAAGTGAGAAATAAGGTTGCAGGAACAATTGGGGAAGCTTATTTGGCATATAATAGGGTAACCGGAGAATTCTTCGATGCTGCTGGGGATATGAAACTTAAGAAACCATCATCTTGA

Homology

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BLAST of CSPI01G25250 vs. Swiss-Prot
Match: TWIH_ARATH (Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=At1g30680 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.1e-26
Identity = 52/76 (68.42%), Postives = 65/76 (85.53%), Query Frame = 1


Query: 524 LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG 583
           LQ+W G  PN++DISG+AHFINKCDNGI++HRNRD  +GP+DLVQ+ VRKVRNKVAG IG
Sbjct: 619 LQHWDGGAPNLYDISGSAHFINKCDNGIIVHRNRDENAGPLDLVQIGVRKVRNKVAGQIG 678

Query: 584 EAYLAYNRVTGEFFDA 600
           +AYL Y+R TG + D+
Sbjct: 679 DAYLCYDRTTGSYSDS 694

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BLAST of CSPI01G25250 vs. TrEMBL
Match: A0A0A0LVZ4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G541900 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 9.2e-45
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 1


Query: 521 ICALQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAG 580
           +  LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAG
Sbjct: 4   VLKLQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAG 63

Query: 581 TIGEAYLAYNRVTGEFFDAAGDMKLKKPSS 611
           TIGEAYLAYNRVTGEFFDAAGDMKLKKPSS
Sbjct: 64  TIGEAYLAYNRVTGEFFDAAGDMKLKKPSS 93

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BLAST of CSPI01G25250 vs. TrEMBL
Match: A0A0A0LYM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G541890 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 2.4e-40
Identity = 81/87 (93.10%), Postives = 84/87 (96.55%), Query Frame = 1


Query: 524 LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG 583
           LQNWSG PPNM+DISG+AHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG
Sbjct: 631 LQNWSGSPPNMYDISGSAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG 690

Query: 584 EAYLAYNRVTGEFFDAAGDMKLKKPSS 611
           EAYL YNRVTGEF DAAGD+KLKKPSS
Sbjct: 691 EAYLEYNRVTGEFLDAAGDVKLKKPSS 717

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BLAST of CSPI01G25250 vs. TrEMBL
Match: A0A151U482_CAJCA (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan GN=KK1_006768 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 6.0e-36
Identity = 95/220 (43.18%), Postives = 121/220 (55.00%), Query Frame = 1


Query: 153 NQGYLNPHFLHHNDNTSLVLATEQLTEENYVSWTQAMTIGLSVKNKIGFVDETITK--RT 212
           +Q   NP FLHH+D   LVL ++ L  +NY +W++AM + L VKNK+ F+D T+ K   T
Sbjct: 9   SQDVSNPLFLHHSDGPGLVLTSQPLDHKNYTTWSRAMQVALFVKNKLAFIDGTLPKPAST 68

Query: 213 GDLLPAWIR-NNIVISWILNSVSKPVSASILFSGSTRAIWIDLKERFQKKNVPRIFQLKR 272
                AW   NN+VISW+ NSVSK +  SILF+ + + IW DLK RF KKN  RIFQL+R
Sbjct: 69  DSTFVAWNHANNVVISWLYNSVSKDIITSILFASTAQEIWHDLKTRFSKKNGSRIFQLRR 128

Query: 273 SLATLEQNQDTI---------------GVNDRF--------------PPNVISHGLMGLN 332
            L +L Q  D I               G    F                  +   LMGLN
Sbjct: 129 QLMSLHQGMDDISTYYTKLKSIWEELSGYKPTFQCTCGGLQQLQSFTESEYVMSFLMGLN 188

Query: 333 ENISQARAQLLLMDPLPSTSRAFSLLLQEEQQRSIGSFSS 341
           ++ISQ R Q+LL DPLPS    FSL+LQ+E QR I   SS
Sbjct: 189 DSISQIRGQILLSDPLPSIGNVFSLVLQDEAQREIAVTSS 228

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BLAST of CSPI01G25250 vs. TrEMBL
Match: A0A151RRY1_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_033170 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 5.1e-35
Identity = 100/250 (40.00%), Postives = 135/250 (54.00%), Query Frame = 1


Query: 158 NPHFLHHNDNTSLVLATEQLTEENYVSWTQAMTIGLSVKNKIGFVDETITKRTGD--LLP 217
           NP FLHH+D   LVL ++ L  +NY +W+ AM +  SVKNKI FVD ++ K   +    P
Sbjct: 14  NPLFLHHSDGPGLVLTSQPLDNKNYTTWSHAMLVAFSVKNKIPFVDGSLPKLAANHPTYP 73

Query: 218 AWIR-NNIVISWILNSVSKPVSASILFSGSTRAIWIDLKERFQKKNVPRIFQLKRSLATL 277
           AWIR NN+VISW+ NSVSK +  SILF+ + + IW DLK +F +KN P IFQL+R L +L
Sbjct: 74  AWIRGNNLVISWLYNSVSKDIITSILFANTAKEIWDDLKTKFSRKNGPHIFQLRRQLMSL 133

Query: 278 EQNQDTI---------------GVNDRFP--------------PNVISHGLMGLNENISQ 337
           +Q  D +               G    FP                 +   LMGLN++ SQ
Sbjct: 134 QQGIDYVSTYYTKLKSIWEELSGYKPSFPCTCGGLQHLQDYNASEYVMSFLMGLNDSFSQ 193

Query: 338 ARAQLLLMDPLPSTSRAFSLLLQEEQQRSIGSFSSTAPTMAFAISVTSSMDTLLDIRPSN 376
            R Q+LL  PLP     FSL+LQEE Q  IG+  +  P++ F      SM  L  +  SN
Sbjct: 194 IRGQILLSYPLPPIGNVFSLILQEETQIEIGTNITHTPSVNF-----DSMAFL--VNSSN 253

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BLAST of CSPI01G25250 vs. TrEMBL
Match: A5BJ98_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_009790 PE=4 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 6.6e-35
Identity = 90/246 (36.59%), Postives = 143/246 (58.13%), Query Frame = 1


Query: 158 NPHFLHHNDNTSLVLATEQLTEENYVSWTQAMTIGLSVKNKIGFVDETITKRTGDLLP-- 217
           NP+FLHH+D+  +VL ++ L  +NY +W +AMTI L+ K+K+GF+D T T  +    P  
Sbjct: 16  NPYFLHHSDHPGMVLVSKPLNGDNYSTWCRAMTISLNAKSKLGFIDGTTTMSSATDKPDE 75

Query: 218 --AWIR-NNIVISWILNSVSKPVSASILFSGSTRAIWIDLKERFQKKNVPRIFQLKRSLA 277
             +W + N++++SWILNS+S+ ++ S++FS + + +W DL++RF + N PRIFQ++R +A
Sbjct: 76  HASWKKCNDMILSWILNSLSQDLADSVIFSTTAQEVWEDLRDRFSQSNAPRIFQIERDIA 135

Query: 278 TLEQNQDTIGVN-----------DRFPPNVISHG-----------LMGLNENISQARAQL 337
            L Q+Q T+                +   V S G           LMGLNE+ +  R Q+
Sbjct: 136 CLTQDQMTVAAYYTRLKKLWDELGSYNDTVCSCGADHKRRRLMQFLMGLNESYNAIRGQI 195

Query: 338 LLMDPLPSTSRAFSLLLQEEQQRSIGSFSSTAPTMAFAISVTSSMDTLLDIRPSNNSIAT 377
           LLM+PLP  +RA+S ++QEE+QRS+G+   T    A  +     M   L +R    S + 
Sbjct: 196 LLMNPLPDVARAYSSIVQEEKQRSLGATRETTENSAMVVQRAEPM--ALAVRHGQGSSSR 255

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BLAST of CSPI01G25250 vs. TAIR10
Match: AT1G30680.1 (AT1G30680.1 toprim domain-containing protein)

HSP 1 Score: 122.1 bits (305), Expect = 1.2e-27
Identity = 52/76 (68.42%), Postives = 65/76 (85.53%), Query Frame = 1


Query: 524 LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG 583
           LQ+W G  PN++DISG+AHFINKCDNGI++HRNRD  +GP+DLVQ+ VRKVRNKVAG IG
Sbjct: 619 LQHWDGGAPNLYDISGSAHFINKCDNGIIVHRNRDENAGPLDLVQIGVRKVRNKVAGQIG 678

Query: 584 EAYLAYNRVTGEFFDA 600
           +AYL Y+R TG + D+
Sbjct: 679 DAYLCYDRTTGSYSDS 694

[Close]

BLAST of CSPI01G25250 vs. TAIR10
Match: AT1G21280.1 (AT1G21280.1 Retrotransposon gag protein (InterPro:IPR005162))

HSP 1 Score: 67.8 bits (164), Expect = 2.7e-11
Identity = 40/129 (31.01%), Postives = 69/129 (53.49%), Query Frame = 1


Query: 156 YLNPHFLHHNDNTSLVLATEQLTEENYVSWTQAMTIGLSVKNKIGFVDETITKRT--GDL 215
           Y  P  +HH  + S+   ++   E+NYV+W       L V  K GF+D T+ K      L
Sbjct: 18  YYLPPDIHHPSDFSIQKLSKD--EDNYVAWKIRFRSFLRVTKKFGFIDGTLPKPDPFSPL 77

Query: 216 LPAWIR-NNIVISWILNSVSKPVSASILFSGSTRAIWIDLKERFQKKNVPRIFQLKRSLA 275
              W + N +V+ W++NS++  +  S++++ +   +W DL+  F      +I+QL+R LA
Sbjct: 78  YQPWEQCNAMVMYWLMNSMTDKLLESVMYAETAHKMWEDLRRVFVPCVDLKIYQLRRRLA 137

Query: 276 TLEQNQDTI 282
           TL Q  D++
Sbjct: 138 TLRQGGDSV 144

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BLAST of CSPI01G25250 vs. NCBI nr
Match: gi|778668587|ref|XP_011649121.1| (PREDICTED: uncharacterized protein LOC105434586 [Cucumis sativus])

HSP 1 Score: 230.3 bits (586), Expect = 8.8e-57
Identity = 112/138 (81.16%), Postives = 124/138 (89.86%), Query Frame = 1


Query: 148 TLFNQNQGYLNPHFLHHNDNTSLVLATEQLTEENYVSWTQAMTIGLSVKNKIGFVDETIT 207
           T F+QNQGYLNP+FLHHNDNT+LVL TEQLTEENYVSW++AMTIGLSVKNKIGFVD TI 
Sbjct: 30  TSFDQNQGYLNPYFLHHNDNTNLVLVTEQLTEENYVSWSRAMTIGLSVKNKIGFVDGTIA 89

Query: 208 KRTGDLLPAWIR-NNIVISWILNSVSKPVSASILFSGSTRAIWIDLKERFQKKNVPRIFQ 267
           + TGDLLP WIR NNIVISWILNSVSKP+SA+ILFS   R IW++LKERFQKKN PRIFQ
Sbjct: 90  RPTGDLLPVWIRNNNIVISWILNSVSKPISANILFSDLARTIWVELKERFQKKNAPRIFQ 149

Query: 268 LKRSLATLEQNQDTIGVN 285
           LKRSLATL QNQD+IG +
Sbjct: 150 LKRSLATLSQNQDSIGTS 167

[Close]

BLAST of CSPI01G25250 vs. NCBI nr
Match: gi|700210852|gb|KGN65948.1| (hypothetical protein Csa_1G541900 [Cucumis sativus])

HSP 1 Score: 189.9 bits (481), Expect = 1.3e-44
Identity = 87/90 (96.67%), Postives = 88/90 (97.78%), Query Frame = 1


Query: 521 ICALQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAG 580
           +  LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAG
Sbjct: 4   VLKLQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAG 63

Query: 581 TIGEAYLAYNRVTGEFFDAAGDMKLKKPSS 611
           TIGEAYLAYNRVTGEFFDAAGDMKLKKPSS
Sbjct: 64  TIGEAYLAYNRVTGEFFDAAGDMKLKKPSS 93

[Close]

BLAST of CSPI01G25250 vs. NCBI nr
Match: gi|778662022|ref|XP_011659237.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X1 [Cucumis sativus])

HSP 1 Score: 189.1 bits (479), Expect = 2.3e-44
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = 1


Query: 524 LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG 583
           LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG
Sbjct: 628 LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG 687

Query: 584 EAYLAYNRVTGEFFDAAGDMKLKKPSS 611
           EAYLAYNRVTGEFFDAAGDMKLKKPSS
Sbjct: 688 EAYLAYNRVTGEFFDAAGDMKLKKPSS 714

[Close]

BLAST of CSPI01G25250 vs. NCBI nr
Match: gi|659121592|ref|XP_008460736.1| (PREDICTED: uncharacterized protein LOC103499498 [Cucumis melo])

HSP 1 Score: 185.3 bits (469), Expect = 3.3e-43
Identity = 108/219 (49.32%), Postives = 134/219 (61.19%), Query Frame = 1


Query: 189 MTIGLSVKNKIGFVDETITKRTGDLLPAWIR-NNIVISWILNSVSKPVSASILFSGSTRA 248
           M IGLSVKNK+GF+D T+ +   DLLP+WIR NNIVISWILNSVSKP+S SILF+ S R+
Sbjct: 1   MIIGLSVKNKLGFIDGTLPRPNDDLLPSWIRNNNIVISWILNSVSKPISTSILFADSARS 60

Query: 249 IWIDLKERFQKKNVPRIFQLKRSLATLEQNQDTIG------------------------- 308
           IW+DLKERFQ+KN PRIF LKRSLA L  NQ+++                          
Sbjct: 61  IWLDLKERFQRKNAPRIFHLKRSLAILSHNQESVSMYFTKFKTLIDELNSYRPACTCGSC 120

Query: 309 -------VNDRFPPNVISHGLMGLNENISQARAQLLLMDPLPSTSRAFSLLLQEEQQRSI 368
                  V +      +   LMGLN++ +Q R QLLLM+P+PS SRAFSLLLQEEQQR+I
Sbjct: 121 RCGENQEVANFLQTEYLMDFLMGLNDSYAQTRTQLLLMEPIPSISRAFSLLLQEEQQRAI 180

Query: 369 GSFSSTAPTMAFAISVTSSMDTLLDIRPSNNSIATQNNE 375
            SFS    T   A++ +S+        P NNS   Q  +
Sbjct: 181 SSFSPAINTPTIALAASSN-------NPKNNSAHKQRKD 212

[Close]

BLAST of CSPI01G25250 vs. NCBI nr
Match: gi|778662016|ref|XP_011659233.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial [Cucumis sativus])

HSP 1 Score: 175.3 bits (443), Expect = 3.4e-40
Identity = 81/87 (93.10%), Postives = 84/87 (96.55%), Query Frame = 1


Query: 524 LQNWSGCPPNMHDISGNAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG 583
           LQNWSG PPNM+DISG+AHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG
Sbjct: 631 LQNWSGSPPNMYDISGSAHFINKCDNGIVIHRNRDPESGPIDLVQVCVRKVRNKVAGTIG 690

Query: 584 EAYLAYNRVTGEFFDAAGDMKLKKPSS 611
           EAYL YNRVTGEF DAAGD+KLKKPSS
Sbjct: 691 EAYLEYNRVTGEFLDAAGDVKLKKPSS 717

The following BLAST results are available for this feature:

BLAST of CSPI01G25250 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TWIH_ARATH	2.1e-26	68.42	Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=...	[more]

BLAST of CSPI01G25250 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVZ4_CUCSA	9.2e-45	96.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G541900 PE=4 SV=1	[more]
A0A0A0LYM8_CUCSA	2.4e-40	93.10	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G541890 PE=4 SV=1	[more]
A0A151U482_CAJCA	6.0e-36	43.18	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan GN=...	[more]
A0A151RRY1_CAJCA	5.1e-35	40.00	Uncharacterized protein OS=Cajanus cajan GN=KK1_033170 PE=4 SV=1	[more]
A5BJ98_VITVI	6.6e-35	36.59	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_009790 PE=4 SV=1	[more]

BLAST of CSPI01G25250 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G30680.1	1.2e-27	68.42	toprim domain-containing protein	[more]
AT1G21280.1	2.7e-11	31.01	Retrotransposon gag protein (InterPro:IPR005162)	[more]

BLAST of CSPI01G25250 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778668587\|ref\|XP_011649121.1\|	8.8e-57	81.16	PREDICTED: uncharacterized protein LOC105434586 [Cucumis sativus]	[more]
gi\|700210852\|gb\|KGN65948.1\|	1.3e-44	96.67	hypothetical protein Csa_1G541900 [Cucumis sativus]	[more]
gi\|778662022\|ref\|XP_011659237.1\|	2.3e-44	100.00	PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X1 ...	[more]
gi\|659121592\|ref\|XP_008460736.1\|	3.3e-43	49.32	PREDICTED: uncharacterized protein LOC103499498 [Cucumis melo]	[more]
gi\|778662016\|ref\|XP_011659233.1\|	3.4e-40	93.10	PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial [Cucumis sativus...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR027032	Twinkle-like protein
IPR027417	P-loop_NTPase

Vocabulary: **Molecular Function**
Term	Definition
GO:0003697	single-stranded DNA binding
GO:0043139	5'-3' DNA helicase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0006260	DNA replication
biological_process	GO:0008150	biological_process
biological_process	GO:0015074	DNA integration
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005657	replication fork
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0043139	5'-3' DNA helicase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0008270	zinc ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G25250.1	CSPI01G25250.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027032	Twinkle-like protein	PANTHER	PTHR12873	T7-LIKE MITOCHONDRIAL DNA HELICASE	coord: 525..598 score: 4.1
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	G3DSA:3.40.50.300		coord: 526..600 score: 2.
None	No IPR available	PANTHER	PTHR12873:SF0	TWINKLE PROTEIN, MITOCHONDRIAL	coord: 525..598 score: 4.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G25250	MELO3C002865	Melon (DHL92) v3.5.1	cpimeB028
CSPI01G25250	MELO3C002865.2	Melon (DHL92) v3.6.1	cpimedB027
CSPI01G25250	Bhi08G000819	Wax gourd	cpiwgoB054
CSPI01G25250	Cucsa.167390	Cucumber (Gy14) v1	cgycpiB262
CSPI01G25250	CmoCh10G012250	Cucurbita moschata (Rifu)	cmocpiB045

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G25250	Cucumber (Chinese Long) v2	cpicuB001
CSPI01G25250	Watermelon (Charleston Gray)	cpiwcgB044
CSPI01G25250	Watermelon (Charleston Gray)	cpiwcgB045
CSPI01G25250	Watermelon (97103) v1	cpiwmB016
CSPI01G25250	Cucurbita pepo (Zucchini)	cpecpiB026
CSPI01G25250	Cucurbita pepo (Zucchini)	cpecpiB508
CSPI01G25250	Bottle gourd (USVL1VR-Ls)	cpilsiB022
CSPI01G25250	Cucumber (Gy14) v2	cgybcpiB002
CSPI01G25250	Silver-seed gourd	carcpiB0430
CSPI01G25250	Silver-seed gourd	carcpiB0485
CSPI01G25250	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G25250	Watermelon (97103) v2	cpiwmbB039
CSPI01G25250	Cucurbita maxima (Rimu)	cmacpiB221
CSPI01G25250	Cucurbita maxima (Rimu)	cmacpiB396
CSPI01G25250	Cucurbita moschata (Rifu)	cmocpiB207
CSPI01G25250	Cucurbita moschata (Rifu)	cmocpiB384