CSPI01G22630 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G22630
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Iron-sulfur cluster assembly protein IscA
Location	Chr1 : 18189601 .. 18194129 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCCCAAACACACGAAGAATGGAGATTAATCTTTTTAACTCATTTTTTTTCATTTAAACTCTCGAAAAAAGAAAAAAGAAAAAAGAAAAAAAAAGCAAAATAAAAATCAAACACAATAATCCCACATCCCAATTCTCAATCGTCCCCTCCGAGGCCCCCGCAAGTCTCCATCAGCCTTCACGCGCCGCCCCGACTCCTCCTGCTCGGTCCCTGCCGCCGTCGCTTCTCCTTCTTTCTCTCCATGCCGCGTCGCAGCTCTGTTCTTCATCTCTGATTGTCGTCGGCTGCTACACCCTTTCGTCTCAGGTCTCAGGTAATAAATTTTGTATATTACAGATTTTTAGATGATTTTTTTCGCTACATCAATTATTACTTTGTTACGTGTTGAGTTAATTGGCTACTTTTACTTCAGTTCTTAGAAACAAGATTGTTGAAATAAGAAATAGGTTAATTTAGATATGTACAGGGAAAGCTATAAATTTCGCAATTCTGAATTCACGTTTTTGGAGAAAACTATTCATAAGCTTGATTTTGGGTCCGAACTGAAACTCACTGGTAATTTCAAACTAAGTTGACTCATTAATTAAAATTGAGGAGAGGATGTAAATATGGATTTGAGAAGAAAGAAGTATCTCATTTTCTGCATGATTTTCTGGTAATTTCAAAATAAGTTGCAATTATAATATTTTGGAAATACAATTAGTAACTTTTCACTCTGCTTCAACTGGTGTGAGTCAGCCGATTGTTTCCTTGTACTAAGTTAGTGCACCATTTCGGAGTATATTTTCTTATAGCCTGAAATATATAGTTAATTTGATTCTTGGTTCATTTGCTCGACCAAATCGTGTATGTGAATTCTCAAATGCTTCAAGATACAGTTTGTTATAATGTTACACGACTTCATACTTTTGCCTCCATTGAGCCAAAATTTGGTCTTACTAAAGCTTGTTCTGCAGTAGTTCGTTCCTCAAACTTAGGATTAACCATATATTCAACAATTGAATTGTCTTTTCTAATGACATTGATAAGCTTGGGTGCACAAGATTTAGTCCTCTTCTAGGGACCAAAGAATACTTATCATTTTTGATTGGGGGAAGAAATGATGTTATAGGAGTTTGAATATATGACTTGCTACTCTGAATCATGATAAATAACCAATGAAACTAAAAGCTAGAAAGTTTAGTTTTTGTTCATATTTTGAAATATAAAAAGTTTAAACATTTGCTCATATTTTTACCATCTATAGTTATTCACATTTTGAATGACTAATTGTAATAATTTGTGTGTTATAGGTAGGTTTCTCTTTTATCTTTTAGCCTCCTATTGAGTATTGAGTTTGGTTTCCCCAGATTTTTATAATCTTAATTGCGGAATCATCTCATGAGCTGAAGATTATGGGAAGTTTCAATCGAAAGAGTGTTTGTTGTTATTGTTGTTTCTTTCTTTTTTTTTTTTAAAAAAATTATATACATATATATATTAATTCTATACAAGTCCTGAAGTCTATATGTTTCTATTTTATGTCCTTATCAGTTATCACATCGAATCTTACGTCCATGTTATATTAATTAAAGTATGATAAAGTTTGGTTTGAAGAAAAAGTCTATGAGCGGAGTGGAAGGGGTTGGATCATGTGTTTTCCATGATTTTATATTTTGTTTTGGAAGGAGAATGAGCACATTTGGAGTGGGTTAATTCAAAATCTTGTTTTCCATTAACATTTTAACTGACTATGATACACTTGAAGGAGCATTTTCCTCATTAATCATGTTAATACCATTAACACATGGATTTTTGAGCAGATAACAATCTAAACTGAAGTGGAGAGCACGAAGGGATGGGCCCTAGATCACTAATTCAGAGGGTAACACCATACTTGGTGGCACGTATCAAGGAGAATCAAAGGCTTCTTAGCTCTTCTGCTTCAGCCTATCAAGAAGCATTGCCTTTGTCTCCATCTCCATCCCCATCTGCCCCTGTTGATACCATTCACATGACTGATAGTTGCATTCGGGTATTCTATATATATATCCTTTTTTGCACATACATTTGGTAGATGAATTCTTCATCCATTTCACTGTTGTCCTCTGGTTTAGTTCTTGATTTTCTGGATTATTCTCTTATGGTGCGCCATCCAGGGCTATAGTTTTTGGGTTTCAGGATCCTAATAAATTGTTGTGCCTTTTAGGTTCTTCAAATTCAATTAATTAAATGAATTTTGAGCATTTCTCTTGTTACCTTAGCAGTTCAACCTATATTGGTGTTCATAGAGCAGTCACGATTTACATTTTTGCTTAGTTTTGACATGCCTTGCAGAGAATGAAAGAATTGCAAGATCCCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGGAAAAACCAATCCTGATGATAGGTTTGACAAATATTAACTCAGTGATTACTGCTTACTTGGTTTTTCACGTAAAGTTTGTCTCTGATGTTTGATTTTACGTAAAAAAAAGTTTCCTCTATTTTTCATGTTTGCAGGATATATGAGAAAGAGGGAGTTAAATTGGTAGTTGATAACATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCTTTTGTTGTAAGTGAAACTTATCTCTCATTGTTGCTGCCTCATTGCTTTTGCTTAGATATGTTTATTTCATACCAATGTGTGGACCATAGCAAATTATAGTTCTATGACATCTATTCAAGTAATGGAGTAATGATAAAGAAAGTTGAAATACTGAAAACAAACTTCCACTAACGCCATCCTTAGAATATTTATCCTTTGACTATTACACCATGCCTTTTATGGAATCTCTCTCCCTTCATAAGGACTGTTTCGGTATCTTATTTAGAGTATTATTTACGTTGAGAAATATACTGAGATGTAAATCCTCCAGCCCTGAGCTGGTAAACTCTTCACACTGTAGATTCTTACCTGATATTTACACTCGATGACCACTCTCGTCTACCTTCCTCATGTTTATGTATGCACTCGACTTCTAACTGTAAGAGGTGGCGTTCTCCATTTTGCTCTTCCTTGAATATATGTGAACTGTAGAATATAACTTAGGAAAAGTTTTCATTGCCTCGTCAGTATGGATACTTCTCTAATTCTTTGTAACTGCTCCAGTTTCATTTTAGAATGATGTGGGTATAGCAACAATCTAAAATGTTTAGTTCAGAAAATTTTTAGTGTGAACAATAAGTAGATGGTGATACTAAAGAATTCTTAAGAATTTCTCCTCGAAGCTACAGCTTGGCTAAAGAAACATTTTATTGATAGATGAAATCAAAGAACTCCAAAACACCAAAGAAAAGAGACAAAGAAAGGTGTTTACAAAAGAGACTGCCAATTAGTAACTAAGAAAGATAAAAGTATTATGTTTGAAAGGAAAAGAAAGAAAAGAAAAAGCAAAAAGAAAGTGATGTACCCTTGAGCAGTACGATCTACAAGTCGTATTTTATCCAAAACTTCCCTATGGAATCCTATCTAACCCATCTTAGGATCTGTTTAGATTGACTAGAGAAAAAATGTTTTTTCAAAAAGTCATTTTTATCCTAGGTTGGCCGTTGGCGGCAACTGGCGAGAGAGATGGCTGGCGTTGGCAACTGGAAGAGGGTTAGGGCAGAGAGGGTTGGTGGTCTCCTGGGGAGAATCGGAGAAGAGAAGGTGGAGTGCGTGAAGCTTTTCGTTTTAAGAGAATAAGAAAAAAAACCCTAAAAATTATTGCCATTGGGCCGGACTAATGTGCCGTTTCTATGGGTTTTTTTTCCAGCCATGTTAGGACCGTGTTCAAAATTTTAAAAAAATCGACATGCCAGCTGGTGTGTCAGTGTTGGAAACGTGTTCGACACTGATACTTGGCCATCTTAGGAGCGTCGATGCTTCTTAGTTTTTATTTAAACTCTTTTGATAAAAATATTTAAAATAAACTTTAAAAGTGTTTCAAAAGCTATTTTGAGTAGTTGGTAAACTCTTTGGTTTTTCTCCAAATAACTGCAAATTAAGTGATTGGAAAGTGAAAACGAACCCACGTGGGATTATTTGCACTGCAGAAGTTGAATTCTTGTTACGTGCAGGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAATCATCTCAAAGCTTCCTTCAGTTGGACCCTTTCCTACAAGGCGTACACAATTATCAACGCTGACCTGAAGGTATGATGAGTATGTTTATCACTCAGTTACACTACTGATTATAAACCTATGTTTTATTTAATCCCATTTTATGTATCATTTTATACATTCATATTTATGTACCACTTCTGTATCATCTGGGAACTGCATTCTTTGTTGCATTGAAATCCAAATAAGCAAAATATAAACTGATTCATCATAATCAAACTTGAACTTAAGACTTCCCTGTTATTCTGATGTTGAGTTTGCAATAATTAATTACACTTT

mRNA sequence

ATGGGCCCTAGATCACTAATTCAGAGGGTAACACCATACTTGGTGGCACGTATCAAGGAGAATCAAAGGCTTCTTAGCTCTTCTGCTTCAGCCTATCAAGAAGCATTGCCTTTGTCTCCATCTCCATCCCCATCTGCCCCTGTTGATACCATTCACATGACTGATAGTTGCATTCGGAGAATGAAAGAATTGCAAGATCCCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGGAAAAACCAATCCTGATGATAGGATATATGAGAAAGAGGGAGTTAAATTGGTAGTTGATAACATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCTTTTGTTGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAA

Coding sequence (CDS)

ATGGGCCCTAGATCACTAATTCAGAGGGTAACACCATACTTGGTGGCACGTATCAAGGAGAATCAAAGGCTTCTTAGCTCTTCTGCTTCAGCCTATCAAGAAGCATTGCCTTTGTCTCCATCTCCATCCCCATCTGCCCCTGTTGATACCATTCACATGACTGATAGTTGCATTCGGAGAATGAAAGAATTGCAAGATCCCAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGGTGTTCTGGATTTCAATATGTTTTCAATTTGGATGGAAAAACCAATCCTGATGATAGGATATATGAGAAAGAGGGAGTTAAATTGGTAGTTGATAACATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTTGAAGAGCTGATTCGTTCTGCTTTTGTTGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAA

Homology

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BLAST of CSPI01G22630 vs. Swiss-Prot
Match: ISAM2_ARATH (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana GN=At5g03905 PE=2 SV=2)

HSP 1 Score: 215.3 bits (547), Expect = 4.8e-55
Identity = 110/155 (70.97%), Postives = 128/155 (82.58%), Query Frame = 1


Query: 4   RSLIQRVTPYLVARIKENQRLLS-SSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRRMK 63
           RSL++RV PYL  RI+EN RLL+ SSASA +EA   S S   S+  D +H++D+CIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQDPK-EEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKG 123
           ELQ  + E+KMLRL VETGGCSGFQY F LD +TNPDDR++EK GVKLVVDN+SYD VKG
Sbjct: 63  ELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVKG 122

Query: 124 ATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 157
           ATIDY EELIR+AFVV+ NPSAVGGCSCKSSFMVK
Sbjct: 123 ATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

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BLAST of CSPI01G22630 vs. Swiss-Prot
Match: ISCA2_BOVIN (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.6e-29
Identity = 74/147 (50.34%), Postives = 94/147 (63.95%), Query Frame = 1


Query: 10  VTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRRMKELQDPKE 69
           VTP+      +  RLL++S      AL      SP A    IH+TDSC++R+ E+ +  E
Sbjct: 15  VTPW------QRSRLLAASRGP--RALREVSFSSPEAGEGQIHLTDSCVQRLLEITEGSE 74

Query: 70  EKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKGATIDYVEE 129
              LRL VE GGCSGFQY F+LD   NPDDR++EK G ++VVD+ S  FVKGA +D+ +E
Sbjct: 75  --FLRLEVEGGGCSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVDFSQE 134

Query: 130 LIRSAFVVSTNPSAVGGCSCKSSFMVK 157
           LIRS+F V  NP A  GCSC SSF VK
Sbjct: 135 LIRSSFQVLNNPQAQQGCSCGSSFSVK 151

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BLAST of CSPI01G22630 vs. Swiss-Prot
Match: ISCA2_PONAB (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.9e-28
Identity = 66/129 (51.16%), Postives = 87/129 (67.44%), Query Frame = 1


Query: 28  SASAYQEALPLSPSPSPSAPVDTIHMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQY 87
           +AS   +A   + S SP A    I +TDSC++R+ E+ +  E   LRL VE GGCSGFQY
Sbjct: 27  AASLGPQARREASSSSPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQY 86

Query: 88  VFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGC 147
            F+LD   NPDDR++E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP A  GC
Sbjct: 87  KFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGC 146

Query: 148 SCKSSFMVK 157
           SC SSF +K
Sbjct: 147 SCGSSFSIK 153

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BLAST of CSPI01G22630 vs. Swiss-Prot
Match: ISCA2_HUMAN (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 2.9e-28
Identity = 66/129 (51.16%), Postives = 87/129 (67.44%), Query Frame = 1


Query: 28  SASAYQEALPLSPSPSPSAPVDTIHMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQY 87
           +AS   +A   + S SP A    I +TDSC++R+ E+ +  E   LRL VE GGCSGFQY
Sbjct: 27  TASLGPQARREASSSSPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQY 86

Query: 88  VFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGC 147
            F+LD   NPDDR++E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP A  GC
Sbjct: 87  KFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGC 146

Query: 148 SCKSSFMVK 157
           SC SSF +K
Sbjct: 147 SCGSSFSIK 153

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BLAST of CSPI01G22630 vs. Swiss-Prot
Match: ISCA2_MOUSE (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus GN=Isca2 PE=1 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 1.4e-27
Identity = 63/116 (54.31%), Postives = 80/116 (68.97%), Query Frame = 1


Query: 41  SPSPSAPVDTIHMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDR 100
           S  P A    I +TDSC++R+ E+ +  E   LRL VE GGCSGFQY F+LD   NPDDR
Sbjct: 40  SSIPEAGEGQIRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQYKFSLDTVINPDDR 99

Query: 101 IYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 157
           ++E+ G ++VVD+ S  FVKGA +D+ +ELIRS+F V  NP A  GCSC SSF VK
Sbjct: 100 VFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSFSVK 153

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BLAST of CSPI01G22630 vs. TrEMBL
Match: A0A0A0LXX8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G496290 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 2.4e-82
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 1


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60
           MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60

Query: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120
           MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157

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BLAST of CSPI01G22630 vs. TrEMBL
Match: A0A0B0MTF5_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_28491 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 5.5e-58
Identity = 116/157 (73.89%), Postives = 135/157 (85.99%), Query Frame = 1


Query: 4   RSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRRMKE 63
           RSLIQR++P+ +ARIK+N +LLSSS S+   AL  + S SPS  +D +HMTD+CI+RMKE
Sbjct: 11  RSLIQRISPFFIARIKQNHKLLSSSFSSSSSALHEASSESPSPSLDAVHMTDNCIKRMKE 70

Query: 64  LQ---DPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 123
           LQ      + KMLRLSVETGGCSGFQYVF+LD KTNPDDR++ +EGVKLVVD+ISYDFVK
Sbjct: 71  LQASEQSSDHKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVFVREGVKLVVDDISYDFVK 130

Query: 124 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           GATIDYVEELIRSAFVV+TNPSAVGGCSCKSSFMVKQ
Sbjct: 131 GATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 167

[Close]

BLAST of CSPI01G22630 vs. TrEMBL
Match: A0A0D2QDP8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G158400 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 7.1e-58
Identity = 115/156 (73.72%), Postives = 136/156 (87.18%), Query Frame = 1


Query: 4   RSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRRMKE 63
           RSLIQR++P+ +ARIK+N +LLSSS S+   AL  + S SPS  +D +HMTD+CI+RMKE
Sbjct: 11  RSLIQRISPFFIARIKQNHKLLSSSFSSSSSALHEASSESPSPSLDAVHMTDNCIKRMKE 70

Query: 64  LQ--DPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKG 123
           LQ  +   ++MLRLSVETGGCSGFQYVF+LD KTNPDDR++ +EGVKLVVD+ISYDFVKG
Sbjct: 71  LQASEQSSDQMLRLSVETGGCSGFQYVFDLDDKTNPDDRVFVREGVKLVVDDISYDFVKG 130

Query: 124 ATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           ATIDYVEELIRSAFVV+TNPSAVGGCSCKSSFMVKQ
Sbjct: 131 ATIDYVEELIRSAFVVTTNPSAVGGCSCKSSFMVKQ 166

[Close]

BLAST of CSPI01G22630 vs. TrEMBL
Match: D7LWV1_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_908267 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.3e-56
Identity = 113/156 (72.44%), Postives = 135/156 (86.54%), Query Frame = 1


Query: 4   RSLIQRVTPYLVARIKENQRLLS-SSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRRMK 63
           RSL++RV PYLV RI+EN RLL+ SSASA +EA   S S S S+ +D +H++D+CIRRMK
Sbjct: 3   RSLVKRVAPYLVGRIRENHRLLNFSSASALKEASSSSSSQSESSSIDVVHLSDNCIRRMK 62

Query: 64  ELQDPK-EEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKG 123
           ELQ  + E+K+LRL VETGGCSGFQYVF LD +TNPDDR++EK GVKLVVDN+SYDFVKG
Sbjct: 63  ELQSSEPEKKLLRLGVETGGCSGFQYVFELDNRTNPDDRVFEKNGVKLVVDNVSYDFVKG 122

Query: 124 ATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           AT+DYVEELIR+AFVV+ NP+AVGGCSCKSSFMVKQ
Sbjct: 123 ATVDYVEELIRAAFVVAENPAAVGGCSCKSSFMVKQ 158

[Close]

BLAST of CSPI01G22630 vs. TrEMBL
Match: A0A061EV31_THECC (Iron-sulfur cluster biosynthesis family protein isoform 1 OS=Theobroma cacao GN=TCM_022590 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 8.7e-56
Identity = 116/164 (70.73%), Postives = 138/164 (84.15%), Query Frame = 1


Query: 4   RSLIQRVTPYLVARIKENQRLLSSSA-------SAYQEALPLSPSPSPSAPVDTIHMTDS 63
           RSLIQR++P+ +ARIK+N +LL SS+       SA  EA P S S SPS+ +D I + D+
Sbjct: 3   RSLIQRISPFFIARIKQNHKLLRSSSYSHNYSSSALHEASPES-SESPSSSLDAIRIADN 62

Query: 64  CIRRMKELQDPKE---EKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDN 123
           C++RMKELQ  +E   EK+LRLSVETGGCSGFQYVF+LD KTNPDDR++E+EGVKLVVDN
Sbjct: 63  CVKRMKELQASEESSHEKLLRLSVETGGCSGFQYVFDLDDKTNPDDRVFEREGVKLVVDN 122

Query: 124 ISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           ISYDFVKGAT+DYVEELIRSAF+V+TNPSAVGGCSCKSSFMVKQ
Sbjct: 123 ISYDFVKGATVDYVEELIRSAFLVTTNPSAVGGCSCKSSFMVKQ 165

[Close]

BLAST of CSPI01G22630 vs. TAIR10
Match: AT5G03905.1 (AT5G03905.1 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 215.3 bits (547), Expect = 2.7e-56
Identity = 110/155 (70.97%), Postives = 128/155 (82.58%), Query Frame = 1


Query: 4   RSLIQRVTPYLVARIKENQRLLS-SSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRRMK 63
           RSL++RV PYL  RI+EN RLL+ SSASA +EA   S S   S+  D +H++D+CIRRMK
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSNDVVHLSDNCIRRMK 62

Query: 64  ELQDPK-EEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVKG 123
           ELQ  + E+KMLRL VETGGCSGFQY F LD +TNPDDR++EK GVKLVVDN+SYD VKG
Sbjct: 63  ELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLVKG 122

Query: 124 ATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 157
           ATIDY EELIR+AFVV+ NPSAVGGCSCKSSFMVK
Sbjct: 123 ATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVK 157

[Close]

BLAST of CSPI01G22630 vs. TAIR10
Match: AT2G16710.3 (AT2G16710.3 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 72.0 bits (175), Expect = 3.7e-13
Identity = 38/117 (32.48%), Postives = 64/117 (54.70%), Query Frame = 1


Query: 44  PSAPVDTIHMTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDR--- 103
           P+     + +TD    R+  L   +++  LRL V+  GC+G  Y  N  GKT+ +D    
Sbjct: 16  PALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLNYAGKTSGNDEKGK 75

Query: 104 ---IYEKEGVKLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM 155
              + E++GV+++V+  +   V G  +D+V++ +RS FV   NP++ G C C  SFM
Sbjct: 76  FDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPNSQGQCGCGESFM 131

[Close]

BLAST of CSPI01G22630 vs. TAIR10
Match: AT2G36260.1 (AT2G36260.1 Iron-sulphur cluster biosynthesis family protein)

HSP 1 Score: 69.3 bits (168), Expect = 2.4e-12
Identity = 36/101 (35.64%), Postives = 60/101 (59.41%), Query Frame = 1


Query: 53  MTDSCIRRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVD 112
           ++D+   R+++L   +++  LRL+VE  GC+G  YV N   +    D + E++GVK++VD
Sbjct: 8   LSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKILVD 67

Query: 113 NISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSF 154
             +   V G  +D+V++ +RS FV   NP+A   C C  SF
Sbjct: 68  PKAVMHVIGTEMDFVDDKLRSEFVF-VNPNAT-KCGCGESF 106

[Close]

BLAST of CSPI01G22630 vs. NCBI nr
Match: gi|449470384|ref|XP_004152897.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 312.8 bits (800), Expect = 3.5e-82
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 1


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60
           MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60

Query: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120
           MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 157

[Close]

BLAST of CSPI01G22630 vs. NCBI nr
Match: gi|659115364|ref|XP_008457520.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 298.9 bits (764), Expect = 5.2e-78
Identity = 153/159 (96.23%), Postives = 154/159 (96.86%), Query Frame = 1


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLS--PSPSPSAPVDTIHMTDSCI 60
           MGPRSLIQRVTPYLVARIKENQRLLSSSA+AYQEA  LS  PSPSPSAPVDTIHMTDSCI
Sbjct: 134 MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSPSAPVDTIHMTDSCI 193

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKT PDDRIYEKEGVKLVVDNISYDF
Sbjct: 194 RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISYDF 253

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 254 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 292

[Close]

BLAST of CSPI01G22630 vs. NCBI nr
Match: gi|659115367|ref|XP_008457521.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 298.9 bits (764), Expect = 5.2e-78
Identity = 153/159 (96.23%), Postives = 154/159 (96.86%), Query Frame = 1


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLS--PSPSPSAPVDTIHMTDSCI 60
           MGPRSLIQRVTPYLVARIKENQRLLSSSA+AYQEA  LS  PSPSPSAPVDTIHMTDSCI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSPSAPVDTIHMTDSCI 60

Query: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120
           RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKT PDDRIYEKEGVKLVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 159

[Close]

BLAST of CSPI01G22630 vs. NCBI nr
Match: gi|449459108|ref|XP_004147288.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 255.0 bits (650), Expect = 8.7e-65
Identity = 130/157 (82.80%), Postives = 141/157 (89.81%), Query Frame = 1


Query: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEALPLSPSPSPSAPVDTIHMTDSCIRR 60
           M PR LIQR+TPYL A I++NQRLLSSS SAYQEA   S   +PSA +DTIHM+D+CIRR
Sbjct: 1   MVPRFLIQRLTPYLGAHIRKNQRLLSSSVSAYQEAS--SSLITPSAGIDTIHMSDNCIRR 60

Query: 61  MKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDFVK 120
           MKELQ+P EEKMLRLSVE GGCSGFQYVFNLD KTNPDDR+ EKEGVKLVVDNISYDFVK
Sbjct: 61  MKELQEPMEEKMLRLSVENGGCSGFQYVFNLDDKTNPDDRVCEKEGVKLVVDNISYDFVK 120

Query: 121 GATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           GATIDYVEELIRSAF+V+TNPSAVGGCSCKSSFMVKQ
Sbjct: 121 GATIDYVEELIRSAFIVTTNPSAVGGCSCKSSFMVKQ 155

[Close]

BLAST of CSPI01G22630 vs. NCBI nr
Match: gi|1009161173|ref|XP_015898754.1| (PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Ziziphus jujuba])

HSP 1 Score: 240.4 bits (612), Expect = 2.2e-60
Identity = 123/162 (75.93%), Postives = 141/162 (87.04%), Query Frame = 1


Query: 1   MGPRSLI-QRVTPYLVARIKENQRLLSS-SASAYQEALPLSPSPSPSAPVDTIHMTDSCI 60
           M PRSLI  R+TPY  ARI++NQRLLSS S+SA QE    SPSPSPS  +D + MT++CI
Sbjct: 60  MAPRSLILHRLTPYFAARIRQNQRLLSSYSSSALQEVSSSSPSPSPSPSLDAVDMTETCI 119

Query: 61  RRMKELQDPK---EEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNIS 120
           RR+K+LQ  +   EEKMLRLSVETGGCSGFQYVF+LD KTNPDDR++E+EGVKL+VDNIS
Sbjct: 120 RRIKDLQSSEPSSEEKMLRLSVETGGCSGFQYVFDLDNKTNPDDRVFEREGVKLIVDNIS 179

Query: 121 YDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVKQ 158
           YDFVKGAT+DYVEELIRSAFVV+TNPSAVGGCSCKSSFMVKQ
Sbjct: 180 YDFVKGATVDYVEELIRSAFVVATNPSAVGGCSCKSSFMVKQ 221

The following BLAST results are available for this feature:

BLAST of CSPI01G22630 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ISAM2_ARATH	4.8e-55	70.97	Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ...	[more]
ISCA2_BOVIN	2.6e-29	50.34	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus GN=ISCA2 PE=...	[more]
ISCA2_PONAB	2.9e-28	51.16	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii GN=ISCA2 P...	[more]
ISCA2_HUMAN	2.9e-28	51.16	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens GN=ISCA2 P...	[more]
ISCA2_MOUSE	1.4e-27	54.31	Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus GN=Isca2 P...	[more]

BLAST of CSPI01G22630 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LXX8_CUCSA	2.4e-82	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G496290 PE=4 SV=1	[more]
A0A0B0MTF5_GOSAR	5.5e-58	73.89	Uncharacterized protein OS=Gossypium arboreum GN=F383_28491 PE=4 SV=1	[more]
A0A0D2QDP8_GOSRA	7.1e-58	73.72	Uncharacterized protein OS=Gossypium raimondii GN=B456_002G158400 PE=4 SV=1	[more]
D7LWV1_ARALL	1.3e-56	72.44	Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRA...	[more]
A0A061EV31_THECC	8.7e-56	70.73	Iron-sulfur cluster biosynthesis family protein isoform 1 OS=Theobroma cacao GN=...	[more]

BLAST of CSPI01G22630 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G03905.1	2.7e-56	70.97	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.3	3.7e-13	32.48	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	2.4e-12	35.64	Iron-sulphur cluster biosynthesis family protein	[more]

BLAST of CSPI01G22630 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449470384\|ref\|XP_004152897.1\|	3.5e-82	100.00	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucumis sati...	[more]
gi\|659115364\|ref\|XP_008457520.1\|	5.2e-78	96.23	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [C...	[more]
gi\|659115367\|ref\|XP_008457521.1\|	5.2e-78	96.23	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X2 [C...	[more]
gi\|449459108\|ref\|XP_004147288.1\|	8.7e-65	82.80	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial isoform X1 [C...	[more]
gi\|1009161173\|ref\|XP_015898754.1\|	2.2e-60	75.93	PREDICTED: iron-sulfur assembly protein IscA-like 2, mitochondrial [Ziziphus juj...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000361	FeS_biogenesis
IPR016092	FeS_cluster_insertion

Vocabulary: **Molecular Function**
Term	Definition
GO:0005198	structural molecule activity
GO:0051536	iron-sulfur cluster binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016226	iron-sulfur cluster assembly

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0019243	methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005198	structural molecule activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G22630.1	CSPI01G22630.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000361	FeS cluster biogenesis	GENE3D	G3DSA:2.60.300.12		coord: 50..141 score: 2.8
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 51..150 score: 1.0
IPR000361	FeS cluster biogenesis	unknown	SSF89360	HesB-like domain	coord: 50..143 score: 3.92
IPR016092	FeS cluster insertion protein	TIGRFAMs	TIGR00049	TIGR00049	coord: 51..155 score: 2.9
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 3..156 score: 3.3
None	No IPR available	PANTHER	PTHR10072:SF46	IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL	coord: 3..156 score: 3.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G22630	Cla008271	Watermelon (97103) v1	cpiwmB023
CSPI01G22630	Csa1G496290	Cucumber (Chinese Long) v2	cpicuB001
CSPI01G22630	MELO3C020686	Melon (DHL92) v3.5.1	cpimeB025
CSPI01G22630	ClCG03G001800	Watermelon (Charleston Gray)	cpiwcgB050
CSPI01G22630	Lsi02G013830	Bottle gourd (USVL1VR-Ls)	cpilsiB031
CSPI01G22630	MELO3C020686.2	Melon (DHL92) v3.6.1	cpimedB024
CSPI01G22630	CsaV3_1G033770	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G22630	Cla97C03G052790	Watermelon (97103) v2	cpiwmbB044
CSPI01G22630	Bhi08G000573	Wax gourd	cpiwgoB048
CSPI01G22630	Cucsa.213580	Cucumber (Gy14) v1	cgycpiB328
CSPI01G22630	CmaCh13G001490	Cucurbita maxima (Rimu)	cmacpiB220
CSPI01G22630	CmoCh13G001540	Cucurbita moschata (Rifu)	cmocpiB206
CSPI01G22630	Cp4.1LG20g08750	Cucurbita pepo (Zucchini)	cpecpiB507
CSPI01G22630	CsGy1G022180	Cucumber (Gy14) v2	cgybcpiB002
CSPI01G22630	Carg21025	Silver-seed gourd	carcpiB0762

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G22630	Watermelon (97103) v2	cpiwmbB025
CSPI01G22630	Watermelon (97103) v2	cpiwmbB058
CSPI01G22630	Wax gourd	cpiwgoB018
CSPI01G22630	Wild cucumber (PI 183967)	cpicpiB024
CSPI01G22630	Wild cucumber (PI 183967)	cpicpiB046
CSPI01G22630	Cucumber (Gy14) v1	cgycpiB053
CSPI01G22630	Cucurbita maxima (Rimu)	cmacpiB430
CSPI01G22630	Cucurbita maxima (Rimu)	cmacpiB544
CSPI01G22630	Cucurbita maxima (Rimu)	cmacpiB584
CSPI01G22630	Cucurbita moschata (Rifu)	cmocpiB420
CSPI01G22630	Cucurbita moschata (Rifu)	cmocpiB572
CSPI01G22630	Cucurbita moschata (Rifu)	cmocpiB573
CSPI01G22630	Cucumber (Chinese Long) v2	cpicuB032
CSPI01G22630	Melon (DHL92) v3.5.1	cpimeB084
CSPI01G22630	Watermelon (Charleston Gray)	cpiwcgB032
CSPI01G22630	Watermelon (Charleston Gray)	cpiwcgB062
CSPI01G22630	Watermelon (97103) v1	cpiwmB069
CSPI01G22630	Watermelon (97103) v1	cpiwmB071
CSPI01G22630	Cucurbita pepo (Zucchini)	cpecpiB267
CSPI01G22630	Cucurbita pepo (Zucchini)	cpecpiB536
CSPI01G22630	Bottle gourd (USVL1VR-Ls)	cpilsiB007
CSPI01G22630	Melon (DHL92) v3.6.1	cpimedB010
CSPI01G22630	Cucumber (Gy14) v2	cgybcpiB159
CSPI01G22630	Cucumber (Gy14) v2	cgybcpiB253
CSPI01G22630	Silver-seed gourd	carcpiB0336
CSPI01G22630	Silver-seed gourd	carcpiB0337
CSPI01G22630	Silver-seed gourd	carcpiB0476
CSPI01G22630	Cucumber (Chinese Long) v3	cpicucB058
CSPI01G22630	Cucumber (Chinese Long) v3	cpicucB037