CSPI01G05370 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI01G05370
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCystatin/monellin superfamily protein
LocationChr1 : 3475669 .. 3476039 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTCCTTACAATACACCTGAGGTTCAAGGATTAGCAGAAAAAGCTATTGAACATTATAACAATCAACATGTATGTTCATATAACATTTTGCCCTACACCATATTTTATATATATATATAATTGTCTAATTAATGATGTTTGATTAATTATTTCATATATATATATAGAGTACAAATTATGAGGTTGTGAATATTGTGAAGGCTAATTATCAAGCTGCATGTGGTATCTTGTATTACGTAACCTTTGATGTTAAAGAAGCTGGAGCAGCTCCTGAGTTACCAATGACTACCTTCCAAGCTCGAGTTTTGCATGGCATTCGTGTTGTAGAGGTTGAACTCTGTCGTCCAGAACCCCCTCCTAAGAATTAA

mRNA sequence

ATGTTTCCTTACAATACACCTGAGGTTCAAGGATTAGCAGAAAAAGCTATTGAACATTATAACAATCAACATAGTACAAATTATGAGGTTGTGAATATTGTGAAGGCTAATTATCAAGCTGCATGTGGTATCTTGTATTACGTAACCTTTGATGTTAAAGAAGCTGGAGCAGCTCCTGAGTTACCAATGACTACCTTCCAAGCTCGAGTTTTGCATGGCATTCGTGTTGTAGAGGTTGAACTCTGTCGTCCAGAACCCCCTCCTAAGAATTAA

Coding sequence (CDS)

ATGTTTCCTTACAATACACCTGAGGTTCAAGGATTAGCAGAAAAAGCTATTGAACATTATAACAATCAACATAGTACAAATTATGAGGTTGTGAATATTGTGAAGGCTAATTATCAAGCTGCATGTGGTATCTTGTATTACGTAACCTTTGATGTTAAAGAAGCTGGAGCAGCTCCTGAGTTACCAATGACTACCTTCCAAGCTCGAGTTTTGCATGGCATTCGTGTTGTAGAGGTTGAACTCTGTCGTCCAGAACCCCCTCCTAAGAATTAA
Homology
[Close]
BLAST of CSPI01G05370 vs. TrEMBL
Match: A0A0A0LVE8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G032460 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 7.3e-23
Identity = 55/66 (83.33%), Postives = 61/66 (92.42%), Query Frame = 1 

Query: 27 NYEVVNIVK--ANYQAACGILYYVTFDVKEAGAAPELPMTTFQARVLHGIRVVEVELCRP 86
          NYE V+  +  AN+QAACGILYY+TFDVKEAGAAPELPMTTFQARVL+GIRV+EVELCRP
Sbjct: 30 NYEPVHESEREANHQAACGILYYITFDVKEAGAAPELPMTTFQARVLYGIRVIEVELCRP 89

Query: 87 EPPPKN 91
          EPPPKN
Sbjct: 90 EPPPKN 95

[Close]
BLAST of CSPI01G05370 vs. TrEMBL
Match: D7KU07_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_338056 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 7.4e-07
Identity = 31/76 (40.79%), Postives = 46/76 (60.53%), Query Frame = 1 

Query: 12  LAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQARVL 71
           LA++A++ YN +H T YE+V +VKANY  AC I++ +TF VK+     +  +  FQ RV 
Sbjct: 68  LAQEALDVYNGRHVTEYELVKVVKANYHFACAIMFLITFQVKD---PYDNMIKLFQTRVR 127

Query: 72  HGIRV-VEVELCRPEP 87
            G  +      CRP+P
Sbjct: 128 QGKHITTHYVFCRPKP 140

[Close]
BLAST of CSPI01G05370 vs. TrEMBL
Match: A0A087G8X4_ARAAL (Uncharacterized protein OS=Arabis alpina GN=AALP_AA8G233100 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 7.4e-07
Identity = 31/79 (39.24%), Postives = 50/79 (63.29%), Query Frame = 1 

Query: 9   VQGLAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQA 68
           ++ L+ K+++ YN+ + T YE V +VKAN+  + GI++ +TF+VK+   A +  +  FQA
Sbjct: 56  LKSLSLKSLDDYNHANETEYEFVKVVKANFHVSAGIMFLITFEVKD---AYDKLIKLFQA 115

Query: 69  RVLH-GIRVVEVELCRPEP 87
           RV H  I   E   CRP+P
Sbjct: 116 RVRHVQINFTEYVFCRPKP 131

[Close]
BLAST of CSPI01G05370 vs. TrEMBL
Match: Q9CAM3_ARATH (Cystatin-related domain protein OS=Arabidopsis thaliana GN=F16M19.10 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 9.6e-07
Identity = 29/79 (36.71%), Postives = 48/79 (60.76%), Query Frame = 1 

Query: 9   VQGLAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQA 68
           ++ LA++A++ +N +H T YE V +VKAN+  AC +++ +TF VK+     +  +  FQ 
Sbjct: 96  LKSLAQEALDDHNGRHGTEYEFVKVVKANFHFACAMMFLITFQVKD---PYDDMVKLFQT 155

Query: 69  RVLHGIRV-VEVELCRPEP 87
           RV HG  +      CRP+P
Sbjct: 156 RVRHGKHITTNYVFCRPKP 171

[Close]
BLAST of CSPI01G05370 vs. TrEMBL
Match: A0A0V0HE28_SOLCH (Putative multicystatin-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 8.1e-06
Identity = 34/76 (44.74%), Postives = 46/76 (60.53%), Query Frame = 1 

Query: 12  LAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTF---DVKEAGAAPELPMTTFQA 71
           L+ KAIEH+N++H+TNY+ V IV  N   A GI  Y+TF   D  ++ A+      TFQA
Sbjct: 109 LSHKAIEHFNSEHNTNYKFVEIVTLNTSPAAGIWCYITFLATDSDDSNAS-----KTFQA 168

Query: 72  RVLHGI-RVVEVELCR 84
            V  GI + +EV  CR
Sbjct: 169 LVWRGINKKIEVRFCR 179

[Close]
BLAST of CSPI01G05370 vs. TAIR10
Match: AT1G63200.1 (AT1G63200.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 4.9e-10
Identity = 29/79 (36.71%), Postives = 48/79 (60.76%), Query Frame = 1 

Query: 9   VQGLAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQA 68
           ++ LA++A++ +N +H T YE V +VKAN+  AC +++ +TF VK+     +  +  FQ 
Sbjct: 96  LKSLAQEALDDHNGRHGTEYEFVKVVKANFHFACAMMFLITFQVKD---PYDDMVKLFQT 155

Query: 69  RVLHGIRV-VEVELCRPEP 87
           RV HG  +      CRP+P
Sbjct: 156 RVRHGKHITTNYVFCRPKP 171

[Close]
BLAST of CSPI01G05370 vs. TAIR10
Match: AT1G63190.1 (AT1G63190.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 55.1 bits (131), Expect = 2.7e-08
Identity = 28/79 (35.44%), Postives = 46/79 (58.23%), Query Frame = 1 

Query: 9   VQGLAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQA 68
           ++ L+ KA++ YN +  T +E V +VKAN+   C I++ +TF+V +     +  +  FQ 
Sbjct: 95  LERLSRKALDDYNQESRTQFEFVKVVKANFHFCCAIMFLITFEVVD---PYDNLIKLFQT 154

Query: 69  RVLHGIRVV-EVELCRPEP 87
           RV H   +V E   CRP+P
Sbjct: 155 RVRHAEDIVTEYVFCRPKP 170

[Close]
BLAST of CSPI01G05370 vs. TAIR10
Match: AT2G37435.1 (AT2G37435.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 52.0 bits (123), Expect = 2.3e-07
Identity = 25/75 (33.33%), Postives = 42/75 (56.00%), Query Frame = 1 

Query: 12  LAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQARVL 71
           L  K++EH+N  HST YE V  +KAN+  + G++Y++TF+ K      +     FQA++ 
Sbjct: 99  LCRKSLEHFNEIHSTKYEFVRFIKANHHVSAGMMYFITFEGKLLSNDDD--SKQFQAKLC 158

Query: 72  HGIRVVEVELCRPEP 87
           +     E+  C  +P
Sbjct: 159 YCGGTPEIISCELKP 171

[Close]
BLAST of CSPI01G05370 vs. TAIR10
Match: AT1G50690.1 (AT1G50690.1 Cystatin/monellin superfamily protein)

HSP 1 Score: 47.0 bits (110), Expect = 7.3e-06
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 1 

Query: 9   VQGLAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQA 68
           V  L++ A++ YN+    N E+V  VKAN +   G ++Y+TF+ K+A +  +    TFQA
Sbjct: 94  VHRLSKIALQKYNDDKLENLELVRAVKANRKYGAGFIFYITFEAKDANSHTD--PITFQA 153

Query: 69  --RVLHGIRVV 78
             R L GI  V
Sbjct: 154 AVRYLRGIETV 162

[Close]
BLAST of CSPI01G05370 vs. NCBI nr
Match: gi|700208880|gb|KGN63976.1| (hypothetical protein Csa_1G032460 [Cucumis sativus])

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-22
Identity = 55/66 (83.33%), Postives = 61/66 (92.42%), Query Frame = 1 

Query: 27 NYEVVNIVK--ANYQAACGILYYVTFDVKEAGAAPELPMTTFQARVLHGIRVVEVELCRP 86
          NYE V+  +  AN+QAACGILYY+TFDVKEAGAAPELPMTTFQARVL+GIRV+EVELCRP
Sbjct: 30 NYEPVHESEREANHQAACGILYYITFDVKEAGAAPELPMTTFQARVLYGIRVIEVELCRP 89

Query: 87 EPPPKN 91
          EPPPKN
Sbjct: 90 EPPPKN 95

[Close]
BLAST of CSPI01G05370 vs. NCBI nr
Match: gi|743757185|ref|XP_010920724.1| (PREDICTED: uncharacterized protein LOC105044493 [Elaeis guineensis])

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-09
Identity = 37/80 (46.25%), Postives = 49/80 (61.25%), Query Frame = 1 

Query: 8   EVQGLAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQ 67
           E    +E A+E YN  H TNYE+V +V+ N +   G++YY+TF  K+A AA  +P   FQ
Sbjct: 104 ECADFSELALEKYNKDHGTNYELVEVVRVNARGVAGVIYYITFTAKDAAAADGVP-KVFQ 163

Query: 68  ARVLHGIRV-VEVELCRPEP 87
           A V   IR  VEV LCR +P
Sbjct: 164 ACVKDMIRTGVEVSLCRLKP 182

[Close]
BLAST of CSPI01G05370 vs. NCBI nr
Match: gi|743885794|ref|XP_010910004.1| (PREDICTED: uncharacterized protein LOC105035980 [Elaeis guineensis])

HSP 1 Score: 65.1 bits (157), Expect = 7.3e-08
Identity = 34/75 (45.33%), Postives = 50/75 (66.67%), Query Frame = 1 

Query: 13  AEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQARVLH 72
           +E A++ YNN + TNYE+V +VK N +   G+++Y+TF  K+A A   +P   FQARV++
Sbjct: 109 SELALKKYNNDNGTNYELVEVVKVNMRIVSGVIFYITFTAKDADATDGVP-EVFQARVVN 168

Query: 73  GI-RVVEVELCRPEP 87
            I   VEV LCR +P
Sbjct: 169 LINNGVEVCLCRLKP 182

[Close]
BLAST of CSPI01G05370 vs. NCBI nr
Match: gi|674233561|gb|KFK26326.1| (hypothetical protein AALP_AA8G233100 [Arabis alpina])

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-06
Identity = 31/79 (39.24%), Postives = 50/79 (63.29%), Query Frame = 1 

Query: 9   VQGLAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQA 68
           ++ L+ K+++ YN+ + T YE V +VKAN+  + GI++ +TF+VK+   A +  +  FQA
Sbjct: 56  LKSLSLKSLDDYNHANETEYEFVKVVKANFHVSAGIMFLITFEVKD---AYDKLIKLFQA 115

Query: 69  RVLH-GIRVVEVELCRPEP 87
           RV H  I   E   CRP+P
Sbjct: 116 RVRHVQINFTEYVFCRPKP 131

[Close]
BLAST of CSPI01G05370 vs. NCBI nr
Match: gi|297837089|ref|XP_002886426.1| (hypothetical protein ARALYDRAFT_338056 [Arabidopsis lyrata subsp. lyrata])

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-06
Identity = 31/76 (40.79%), Postives = 46/76 (60.53%), Query Frame = 1 

Query: 12  LAEKAIEHYNNQHSTNYEVVNIVKANYQAACGILYYVTFDVKEAGAAPELPMTTFQARVL 71
           LA++A++ YN +H T YE+V +VKANY  AC I++ +TF VK+     +  +  FQ RV 
Sbjct: 68  LAQEALDVYNGRHVTEYELVKVVKANYHFACAIMFLITFQVKD---PYDNMIKLFQTRVR 127

Query: 72  HGIRV-VEVELCRPEP 87
            G  +      CRP+P
Sbjct: 128 QGKHITTHYVFCRPKP 140

The following BLAST results are available for this feature:
BLAST of CSPI01G05370 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of CSPI01G05370 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LVE8_CUCSA7.3e-2383.33Uncharacterized protein OS=Cucumis sativus GN=Csa_1G032460 PE=4 SV=1[more]
D7KU07_ARALL7.4e-0740.79Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRA... [more]
A0A087G8X4_ARAAL7.4e-0739.24Uncharacterized protein OS=Arabis alpina GN=AALP_AA8G233100 PE=4 SV=1[more]
Q9CAM3_ARATH9.6e-0736.71Cystatin-related domain protein OS=Arabidopsis thaliana GN=F16M19.10 PE=4 SV=1[more]
A0A0V0HE28_SOLCH8.1e-0644.74Putative multicystatin-like OS=Solanum chacoense PE=4 SV=1[more]
BLAST of CSPI01G05370 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G63200.14.9e-1036.71 Cystatin/monellin superfamily protein[more]
AT1G63190.12.7e-0835.44 Cystatin/monellin superfamily protein[more]
AT2G37435.12.3e-0733.33 Cystatin/monellin superfamily protein[more]
AT1G50690.17.3e-0638.03 Cystatin/monellin superfamily protein[more]
BLAST of CSPI01G05370 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700208880|gb|KGN63976.1|1.1e-2283.33hypothetical protein Csa_1G032460 [Cucumis sativus][more]
gi|743757185|ref|XP_010920724.1|1.7e-0946.25PREDICTED: uncharacterized protein LOC105044493 [Elaeis guineensis][more]
gi|743885794|ref|XP_010910004.1|7.3e-0845.33PREDICTED: uncharacterized protein LOC105035980 [Elaeis guineensis][more]
gi|674233561|gb|KFK26326.1|1.1e-0639.24hypothetical protein AALP_AA8G233100 [Arabis alpina][more]
gi|297837089|ref|XP_002886426.1|1.1e-0640.79hypothetical protein ARALYDRAFT_338056 [Arabidopsis lyrata subsp. lyrata][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000010Cystatin_dom
IPR006525Cystatin-related_pln
IPR020381Proteinase inhibitor I25, cystatin, conserved region
Vocabulary: Molecular Function
TermDefinition
GO:0004869cysteine-type endopeptidase inhibitor activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G05370.1CSPI01G05370.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000010Cystatin domainPFAMPF00031Cystatincoord: 3..55
score: 4.2
IPR006525Cystatin-related, plantTIGRFAMsTIGR01638TIGR01638coord: 9..89
score: 5.2
IPR020381Proteinase inhibitor I25, cystatin, conserved regionPRODOMPD001231coord: 6..56
score: 4.
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 4..59
score: 2.6
NoneNo IPR availablePANTHERPTHR31260FAMILY NOT NAMEDcoord: 9..86
score: 3.8
NoneNo IPR availablePANTHERPTHR31260:SF29CYSTATIN-RELATED DOMAIN PROTEIN-RELATEDcoord: 9..86
score: 3.8
NoneNo IPR availableunknownSSF54403Cystatin/monellincoord: 4..65
score: 1.32

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI01G05370Csa1G032460Cucumber (Chinese Long) v2cpicuB000
CSPI01G05370CsaV3_1G005250Cucumber (Chinese Long) v3cpicucB001
CSPI01G05370CsGy1G005130Cucumber (Gy14) v2cgybcpiB001
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI01G05370Cucurbita pepo (Zucchini)cpecpiB519
CSPI01G05370Cucurbita pepo (Zucchini)cpecpiB535
CSPI01G05370Bottle gourd (USVL1VR-Ls)cpilsiB021
CSPI01G05370Melon (DHL92) v3.6.1cpimedB023
CSPI01G05370Silver-seed gourdcarcpiB0421
CSPI01G05370Watermelon (97103) v2cpiwmbB055
CSPI01G05370Wax gourdcpiwgoB034
CSPI01G05370Cucumber (Gy14) v1cgycpiB137
CSPI01G05370Cucurbita maxima (Rimu)cmacpiB208
CSPI01G05370Cucurbita maxima (Rimu)cmacpiB431
CSPI01G05370Cucurbita moschata (Rifu)cmocpiB195
CSPI01G05370Cucurbita moschata (Rifu)cmocpiB421
CSPI01G05370Melon (DHL92) v3.5.1cpimeB024
CSPI01G05370Watermelon (Charleston Gray)cpiwcgB059
CSPI01G05370Watermelon (97103) v1cpiwmB066