Bhi05G000509 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi05G000509
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cysteine proteinase inhibitor
Location	chr5 : 20156371 .. 20157259 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGGGAAGAAGCATCACCACACAAAACTTACTTTCTCTAAATACTAAAGATCATTTATTAAAGAGAGATAGCAAGATGGTTTGTCCAGACAAGAACGTGTGGTACCCATTGCCTGATCTTTCTTTAGATTGTGTGCAAGAAATTGCGAAATTTGCAGTGAAAAAAGGCAGTGACCCAAACAACCCGCTGAATTTGAAGAAAATTACAGACGGATGGTATTTGGAGTTGGATGATGACAGAGTAATCTATCGTTTACGTATCATAGCAACCAACGAGAAAGGAGAAGAAGGGCTATATCAAACAGAGGTTCTTGATGAGTATGTAGATGAGAAAAAAAGACAGTCACTCATTTATTTCCGTCCTGGGGTTATACTTGACGGTGAATTCTGGTTTATAGTTCCTGATGTTGACGATTCTCGTATCCAAGAGATTGCGAAGTTTGCAGTGGCTGAGCATAACAAGAAGGCTAATGATAATCTCGTATACATTTCAACTACTCGCGGCTACTATTTTGATGTGGATGAAATTACTAGAAGGTATAAACTTGTTCTCAAGGCGAAGGATTGTTTGGGACGTGTGCGGGACTATGATGCTTTGGTGCTTGAAGAGAAAACGGATTCTGGGACGACTTTAAAATTTGAATCTTTAAAGATTATCCCATGGAAATGTTGAAAAGACTTGCTATCTAAGTGAGTTTTGTTATGCTATGTGTACGTCCTTGACTATAACTATATATAAATAGTCAAGTGCTTAATAAATAAAGCATTCTAAAATATATCATGCTCTATGACTATGTTTTGTTTTGTTTTGTTTTTTTTTGAAACCGAAATATCGATTGATCGAGCTAAACTATGAGATTAATGAAAACGTAAAAATGTAGAAGTTTTCG

mRNA sequence

AAGGGAAGAAGCATCACCACACAAAACTTACTTTCTCTAAATACTAAAGATCATTTATTAAAGAGAGATAGCAAGATGGTTTGTCCAGACAAGAACGTGTGGTACCCATTGCCTGATCTTTCTTTAGATTGTGTGCAAGAAATTGCGAAATTTGCAGTGAAAAAAGGCAGTGACCCAAACAACCCGCTGAATTTGAAGAAAATTACAGACGGATGGTATTTGGAGTTGGATGATGACAGAGTAATCTATCGTTTACGTATCATAGCAACCAACGAGAAAGGAGAAGAAGGGCTATATCAAACAGAGGTTCTTGATGAGTATGTAGATGAGAAAAAAAGACAGTCACTCATTTATTTCCGTCCTGGGGTTATACTTGACGGTGAATTCTGGTTTATAGTTCCTGATGTTGACGATTCTCGTATCCAAGAGATTGCGAAGTTTGCAGTGGCTGAGCATAACAAGAAGGCTAATGATAATCTCGTATACATTTCAACTACTCGCGGCTACTATTTTGATGTGGATGAAATTACTAGAAGGTATAAACTTGTTCTCAAGGCGAAGGATTGTTTGGGACGTGTGCGGGACTATGATGCTTTGGTGCTTGAAGAGAAAACGGATTCTGGGACGACTTTAAAATTTGAATCTTTAAAGATTATCCCATGGAAATGTTGAAAAGACTTGCTATCTAAGTGAGTTTTGTTATGCTATGTGTACGTCCTTGACTATAACTATATATAAATAGTCAAGTGCTTAATAAATAAAGCATTCTAAAATATATCATGCTCTATGACTATGTTTTGTTTTGTTTTGTTTTTTTTTGAAACCGAAATATCGATTGATCGAGCTAAACTATGAGATTAATGAAAACGTAAAAATGTAGAAGTTTTCG

Coding sequence (CDS)

AAGGGAAGAAGCATCACCACACAAAACTTACTTTCTCTAAATACTAAAGATCATTTATTAAAGAGAGATAGCAAGATGGTTTGTCCAGACAAGAACGTGTGGTACCCATTGCCTGATCTTTCTTTAGATTGTGTGCAAGAAATTGCGAAATTTGCAGTGAAAAAAGGCAGTGACCCAAACAACCCGCTGAATTTGAAGAAAATTACAGACGGATGGTATTTGGAGTTGGATGATGACAGAGTAATCTATCGTTTACGTATCATAGCAACCAACGAGAAAGGAGAAGAAGGGCTATATCAAACAGAGGTTCTTGATGAGTATGTAGATGAGAAAAAAAGACAGTCACTCATTTATTTCCGTCCTGGGGTTATACTTGACGGTGAATTCTGGTTTATAGTTCCTGATGTTGACGATTCTCGTATCCAAGAGATTGCGAAGTTTGCAGTGGCTGAGCATAACAAGAAGGCTAATGATAATCTCGTATACATTTCAACTACTCGCGGCTACTATTTTGATGTGGATGAAATTACTAGAAGGTATAAACTTGTTCTCAAGGCGAAGGATTGTTTGGGACGTGTGCGGGACTATGATGCTTTGGTGCTTGAAGAGAAAACGGATTCTGGGACGACTTTAAAATTTGAATCTTTAAAGATTATCCCATGGAAATGTTGA

Protein sequence

KGRSITTQNLLSLNTKDHLLKRDSKMVCPDKNVWYPLPDLSLDCVQEIAKFAVKKGSDPNNPLNLKKITDGWYLELDDDRVIYRLRIIATNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGEFWFIVPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGRVRDYDALVLEEKTDSGTTLKFESLKIIPWKC

Homology

[Close]

BLAST of Bhi05G000509 vs. Swiss-Prot
Match: sp|Q6TPK4|CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.0e-06
Identity = 30/74 (40.54%), Postives = 47/74 (63.51%), Query Frame = 0


Query: 130 WFIVPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDC 189
           W  +  ++ + +Q++A+FAV+EHNK+AND L Y S  RGY   V      Y+LV+ AKD 
Sbjct: 32  WRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVV--AGTNYRLVIAAKDG 91

Query: 190 LGRVRDYDALVLEE 204
              V +Y+A+V ++
Sbjct: 92  -AVVGNYEAVVWDK 102

[Close]

BLAST of Bhi05G000509 vs. Swiss-Prot
Match: sp|Q84WT8|CYT4_ARATH (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 5.0e-05
Identity = 23/71 (32.39%), Postives = 44/71 (61.97%), Query Frame = 0


Query: 133 VPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGR 192
           + +V D  +  +AK+A+ EHNK++ + LV++    G    V     +Y L + AKD  G+
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVS--GTKYDLKIAAKDGGGK 94

Query: 193 VRDYDALVLEE 204
           +++Y+A+V+E+
Sbjct: 95  IKNYEAVVVEK 103

[Close]

BLAST of Bhi05G000509 vs. TAIR10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 49.7 bits (117), Expect = 2.8e-06
Identity = 23/71 (32.39%), Postives = 44/71 (61.97%), Query Frame = 0


Query: 133 VPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGR 192
           + +V D  +  +AK+A+ EHNK++ + LV++    G    V     +Y L + AKD  G+
Sbjct: 35  IKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVVS--GTKYDLKIAAKDGGGK 94

Query: 193 VRDYDALVLEE 204
           +++Y+A+V+E+
Sbjct: 95  IKNYEAVVVEK 103

[Close]

BLAST of Bhi05G000509 vs. TrEMBL
Match: tr|A0A0A0K3W6|A0A0A0K3W6_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_7G222880 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 9.6e-30
Identity = 75/198 (37.88%), Postives = 112/198 (56.57%), Query Frame = 0


Query: 26  MVCPDKNVWYPLPDLSLDCVQEIAKFAVKKGSDPN-NPLNLKKITDGWYLELDDDRVIYR 85
           M C  K  W  +PD +  CV E+AK  V + +  + + L  K++  GW+ E+DD  + +R
Sbjct: 1   MSCDSKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHR 60

Query: 86  LRIIATNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGE---FWFIVPDVDDSRI 145
           L I     KG   +Y+  ++    D K+ ++LI F PG + +     FW  +PDV  S +
Sbjct: 61  LIIEVVTSKGVVLIYELIIVVVQKDGKRVRTLISFLPGYLDEKNPLIFWIKIPDVKKSCV 120

Query: 146 QEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGRVRDYDALVL 205
           Q+ AK+AV EHNK  +D L Y ST  G+Y +V+     +KL +KAK+C+GRVRDY A+V 
Sbjct: 121 QDCAKWAVTEHNKITHDYLAYDSTVEGWYHEVNPFVIIFKLHIKAKECIGRVRDYIAVVK 180

Query: 206 EEKTDSGTTLKFESLKII 220
           EEK  +    K E   +I
Sbjct: 181 EEKPLTEKIKKLEDFYLI 198

[Close]

BLAST of Bhi05G000509 vs. TrEMBL
Match: tr|A0A0A0LYF4|A0A0A0LYF4_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_1G710160 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.3e-29
Identity = 79/199 (39.70%), Postives = 121/199 (60.80%), Query Frame = 0


Query: 26  MVCPDKNVWYPLPDLSLDCVQEIAKFAV-KKGSDPNNPLNLKKITDGWYLELDDDRVIYR 85
           M C DK  W  +P  +  CV +IA FAV +   +    L LK +  GW+LEL D ++ +R
Sbjct: 1   MSCVDKGEWSKIPVCTTPCVLDIANFAVTEHNKETGEKLTLKSVIKGWFLELGDVKLKFR 60

Query: 86  LRIIATNEKGEEGLYQTEV-LDEYVDEKKRQSLIYFRPGVILDGE-FWFIVPDVDDSRIQ 145
           L I+A N+KG    Y+  V + E  D K+ + L+ F  G + + + FW ++ D   S +Q
Sbjct: 61  LYILAANDKGVVLTYEAVVCVTEIGDFKRVKKLLSFNVGYLDEKDVFWIVIVDTKASCVQ 120

Query: 146 EIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITR-RYKLVLKAKDCLGRVRDYDALVL 205
           ++AKFAVA+HN+  +D+LVY+S T+G+Y ++D      +++ L  KDC GRVR++ ALVL
Sbjct: 121 DVAKFAVAKHNEDEHDSLVYLSITKGWYRELDPYNAIFFEIHLTTKDCFGRVREFKALVL 180

Query: 206 EEK--TDSGTTLK-FESLK 218
           E+K   +   TLK FE +K
Sbjct: 181 EDKPQKEKIRTLKYFEVIK 199

[Close]

BLAST of Bhi05G000509 vs. TrEMBL
Match: tr|A0A1S3BHQ3|A0A1S3BHQ3_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103489701 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.3e-28
Identity = 80/199 (40.20%), Postives = 110/199 (55.28%), Query Frame = 0


Query: 31  KNVWYPLPDLSLDCVQEIAKFAV-KKGSDPNNPLNLKKITDGWYLELDDDRVIYRLRIIA 90
           K  W  + D +  CV    K AV     +  + L  KK+  GWYLE+D ++   RL I  
Sbjct: 7   KEQWIQIHDTTTPCVINFGKHAVDDHNKEKGDNLVFKKVIIGWYLEIDYNKTKLRLIIEV 66

Query: 91  TNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGE---FWFIVPDVDDSRIQEIAK 150
            N KGE   ++  ++ +  D K  +++I F PG I +     FW  + +V +  +QEIAK
Sbjct: 67  VNSKGEVITFEVVIVVDDKDGKTVKTVISFVPGHIDEKNPWTFWIKIHNVTEPHVQEIAK 126

Query: 151 FAVAEHNKKANDNLVYISTTRGYYF--DVDEITRRYKLVLKAKDCLGRVRDYDALVLEEK 210
           FAV EHNK  +DNL+YI    G+Y    +  +T  +KL LKAKDCLGRVR+Y+ALV EEK
Sbjct: 127 FAVEEHNKITHDNLIYICIIEGWYHVSTIPPVT-LWKLHLKAKDCLGRVREYEALVKEEK 186

Query: 211 TDSGTTLKFESLKIIPWKC 224
                 LK E   +IP  C
Sbjct: 187 LLPKHVLKLEHFIVIPKIC 204

[Close]

BLAST of Bhi05G000509 vs. TrEMBL
Match: tr|P94012|P94012_CUCMA (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=PP1 PE=3 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 8.2e-21
Identity = 68/202 (33.66%), Postives = 108/202 (53.47%), Query Frame = 0


Query: 29  PDKNVWYPLPDLSLDCVQEIAKFAVKK-GSDPNNPLNLKKITDGWYLELDDDRVIYRLRI 88
           P    W  +PD+S  CVQE+AK AV++      + L    I +GWY EL  + + YRL I
Sbjct: 12  PAVEKWIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHI 71

Query: 89  IATNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDG------------EFWFIVPD 148
            A +      LY+  V +E    ++ + LI F    +L+G            E W  +PD
Sbjct: 72  RAIDFLQRSLLYEALVFEEKPKLERIRKLISFL--YLLNGGHYVGPVDPPQVEKWIKIPD 131

Query: 149 VDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGRVRD 208
           +    +QE++KFA+ E+N K  + L Y+    G+Y ++ +   +++L +KAKDCLGR+R+
Sbjct: 132 LRVPFVQEVSKFAIDEYN-KGGEGLKYVEIYDGWYAEMGQDNIKFRLHVKAKDCLGRLRN 191

Query: 209 YDALVLEEKTDSGTTLKFESLK 218
           Y+A+VL ++  S      ES K
Sbjct: 192 YEAIVLVKQFLSKKIKILESFK 210

[Close]

BLAST of Bhi05G000509 vs. TrEMBL
Match: tr|Q7DMD5|Q7DMD5_CUCMA (Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=cPC7 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 3.2e-17
Identity = 63/225 (28.00%), Postives = 117/225 (52.00%), Query Frame = 0


Query: 2   GRSITTQNLLSLNTKDHL--LKRDSKMVC----PDKNVWYPLPDLSLDCVQEIAKFAVKK 61
           GR +  + ++    +DHL   K DS +      P +  W  +P++ +  VQE+AKFAV +
Sbjct: 89  GRPLKFEVVIIEEIRDHLRIWKLDSIIAIVSPEPQEKKWIKIPNVQVPLVQELAKFAVDE 148

Query: 62  GSDPNNPLNLKKITDGWYLELDDDRVIYRLRIIATNEKGEEGLYQTEVLDEYVDEKKRQS 121
            +   + L   ++ +GW+++L  D + +RL + A +  G    Y+  VL E+   K+ + 
Sbjct: 149 HNKNGDGLKYIEVYEGWFMDLGHDNIKFRLHLKAKDWLGRIRNYEAVVLVEHFWSKRIKI 208

Query: 122 LIYFR-PGVILDGEFWFIVPDVDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVD 181
           L  F+  G ++    W  +P++ +   Q +  F V +      D L + S   G+YF++ 
Sbjct: 209 LESFKFCGPLVFEPKWIQIPNLKEPGFQVVINFIVEQLKIILGDCLKFHSIYEGWYFELC 268

Query: 182 EITRRYKLVLKAKDCLGRVRDYDALVLEEKTDSGTTLKFESLKII 220
            I+ +++L +KA D LGR  +Y+ +++EEK       K ES+ +I
Sbjct: 269 PISLKFRLHIKAIDFLGRCLNYEIIIIEEK----RIFKLESIIVI 309

[Close]

BLAST of Bhi05G000509 vs. NCBI nr
Match: XP_011659743.1 (PREDICTED: uncharacterized protein LOC101206992 [Cucumis sativus])

HSP 1 Score: 155.6 bits (392), Expect = 2.0e-34
Identity = 85/193 (44.04%), Postives = 119/193 (61.66%), Query Frame = 0


Query: 34  WYPLPDLSLDCVQEIAKFAVKKGSDPN-NPLNLKKITDGWYLELDDDRVIYRLRIIATNE 93
           W  +PD    CV ++ K+AV   +  + + L  KK+  GWYLE+D D+   RL I   N 
Sbjct: 10  WIQIPDTKTPCVIDLGKYAVDDYNKAHKDNLVFKKVIIGWYLEIDYDKTKLRLIIEVVNS 69

Query: 94  KGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGE---FWFIVPDVDDSRIQEIAKFAV 153
           KGE  +Y+  ++ +  D KK ++ I F PG I + +   FWF + D++D  +Q+IAKFAV
Sbjct: 70  KGEVIIYEVVIVVDDKDGKKVKTPISFIPGYIDEVDPWLFWFRIIDINDPHVQQIAKFAV 129

Query: 154 AEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGRVRDYDALVLEEKTDSGT 213
            +HN+  ND+LVYIST   +Y  +  I ++YK+ LKAKDCLGRVRDY+ALV EEK     
Sbjct: 130 EKHNQDNNDSLVYISTIVCWYRLLSPILKQYKVYLKAKDCLGRVRDYEALVEEEKKLPNA 189

Query: 214 TLKFESLKIIPWK 223
            LK    K+IP K
Sbjct: 190 ILKLIYFKVIPKK 202

[Close]

BLAST of Bhi05G000509 vs. NCBI nr
Match: KGN44208.1 (Phloem filament protein [Cucumis sativus])

HSP 1 Score: 139.4 bits (350), Expect = 1.5e-29
Identity = 75/198 (37.88%), Postives = 112/198 (56.57%), Query Frame = 0


Query: 26  MVCPDKNVWYPLPDLSLDCVQEIAKFAVKKGSDPN-NPLNLKKITDGWYLELDDDRVIYR 85
           M C  K  W  +PD +  CV E+AK  V + +  + + L  K++  GW+ E+DD  + +R
Sbjct: 1   MSCDSKKTWIKIPDCTTPCVNEVAKDGVNQHNKASGDSLVYKRVIVGWFWEIDDHAIKHR 60

Query: 86  LRIIATNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGE---FWFIVPDVDDSRI 145
           L I     KG   +Y+  ++    D K+ ++LI F PG + +     FW  +PDV  S +
Sbjct: 61  LIIEVVTSKGVVLIYELIIVVVQKDGKRVRTLISFLPGYLDEKNPLIFWIKIPDVKKSCV 120

Query: 146 QEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGRVRDYDALVL 205
           Q+ AK+AV EHNK  +D L Y ST  G+Y +V+     +KL +KAK+C+GRVRDY A+V 
Sbjct: 121 QDCAKWAVTEHNKITHDYLAYDSTVEGWYHEVNPFVIIFKLHIKAKECIGRVRDYIAVVK 180

Query: 206 EEKTDSGTTLKFESLKII 220
           EEK  +    K E   +I
Sbjct: 181 EEKPLTEKIKKLEDFYLI 198

[Close]

BLAST of Bhi05G000509 vs. NCBI nr
Match: XP_011660324.1 (PREDICTED: uncharacterized protein LOC105436367 [Cucumis sativus] >KGN66905.1 Phloem filament protein [Cucumis sativus])

HSP 1 Score: 139.0 bits (349), Expect = 1.9e-29
Identity = 79/199 (39.70%), Postives = 121/199 (60.80%), Query Frame = 0


Query: 26  MVCPDKNVWYPLPDLSLDCVQEIAKFAV-KKGSDPNNPLNLKKITDGWYLELDDDRVIYR 85
           M C DK  W  +P  +  CV +IA FAV +   +    L LK +  GW+LEL D ++ +R
Sbjct: 1   MSCVDKGEWSKIPVCTTPCVLDIANFAVTEHNKETGEKLTLKSVIKGWFLELGDVKLKFR 60

Query: 86  LRIIATNEKGEEGLYQTEV-LDEYVDEKKRQSLIYFRPGVILDGE-FWFIVPDVDDSRIQ 145
           L I+A N+KG    Y+  V + E  D K+ + L+ F  G + + + FW ++ D   S +Q
Sbjct: 61  LYILAANDKGVVLTYEAVVCVTEIGDFKRVKKLLSFNVGYLDEKDVFWIVIVDTKASCVQ 120

Query: 146 EIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITR-RYKLVLKAKDCLGRVRDYDALVL 205
           ++AKFAVA+HN+  +D+LVY+S T+G+Y ++D      +++ L  KDC GRVR++ ALVL
Sbjct: 121 DVAKFAVAKHNEDEHDSLVYLSITKGWYRELDPYNAIFFEIHLTTKDCFGRVREFKALVL 180

Query: 206 EEK--TDSGTTLK-FESLK 218
           E+K   +   TLK FE +K
Sbjct: 181 EDKPQKEKIRTLKYFEVIK 199

[Close]

BLAST of Bhi05G000509 vs. NCBI nr
Match: XP_008447193.1 (PREDICTED: uncharacterized protein LOC103489701 [Cucumis melo])

HSP 1 Score: 133.7 bits (335), Expect = 8.0e-28
Identity = 80/199 (40.20%), Postives = 110/199 (55.28%), Query Frame = 0


Query: 31  KNVWYPLPDLSLDCVQEIAKFAV-KKGSDPNNPLNLKKITDGWYLELDDDRVIYRLRIIA 90
           K  W  + D +  CV    K AV     +  + L  KK+  GWYLE+D ++   RL I  
Sbjct: 7   KEQWIQIHDTTTPCVINFGKHAVDDHNKEKGDNLVFKKVIIGWYLEIDYNKTKLRLIIEV 66

Query: 91  TNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDGE---FWFIVPDVDDSRIQEIAK 150
            N KGE   ++  ++ +  D K  +++I F PG I +     FW  + +V +  +QEIAK
Sbjct: 67  VNSKGEVITFEVVIVVDDKDGKTVKTVISFVPGHIDEKNPWTFWIKIHNVTEPHVQEIAK 126

Query: 151 FAVAEHNKKANDNLVYISTTRGYYF--DVDEITRRYKLVLKAKDCLGRVRDYDALVLEEK 210
           FAV EHNK  +DNL+YI    G+Y    +  +T  +KL LKAKDCLGRVR+Y+ALV EEK
Sbjct: 127 FAVEEHNKITHDNLIYICIIEGWYHVSTIPPVT-LWKLHLKAKDCLGRVREYEALVKEEK 186

Query: 211 TDSGTTLKFESLKIIPWKC 224
                 LK E   +IP  C
Sbjct: 187 LLPKHVLKLEHFIVIPKIC 204

[Close]

BLAST of Bhi05G000509 vs. NCBI nr
Match: XP_022989567.1 (uncharacterized protein LOC111486626 isoform X2 [Cucurbita maxima])

HSP 1 Score: 111.7 bits (278), Expect = 3.3e-21
Identity = 68/204 (33.33%), Postives = 110/204 (53.92%), Query Frame = 0


Query: 29  PDKNVWYPLPDLSLDCVQEIAKFAVKK-GSDPNNPLNLKKITDGWYLELDDDRVIYRLRI 88
           P    W  +PD+S  CVQE+AK AV++      + L    I +GWY EL  + + YRL I
Sbjct: 12  PAVEKWIKIPDVSGHCVQEVAKLAVEQFNVQHGHSLKYDSIHEGWYCELGQNNLKYRLHI 71

Query: 89  IATNEKGEEGLYQTEVLDEYVDEKKRQSLIYFRPGVILDG------------EFWFIVPD 148
            A +      LY+  V +E    ++ + LI F    +L+G            E W  +PD
Sbjct: 72  RAIDFLQRSLLYEALVFEEKPKLERIRKLISFL--YLLNGGHYVGPVDPPQVEKWIKIPD 131

Query: 149 VDDSRIQEIAKFAVAEHNKKANDNLVYISTTRGYYFDVDEITRRYKLVLKAKDCLGRVRD 208
           +    +QE++KFA+ E+N K  + L Y+    G+Y ++ +   +++L +KAKDCLGR+R+
Sbjct: 132 LRVPFVQEVSKFAIDEYN-KGGEGLKYVEIYDGWYAEMGQDNIKFRLHVKAKDCLGRLRN 191

Query: 209 YDALVLEEKTDSGTTLKFESLKII 220
           Y+A+VL ++  S      ES K++
Sbjct: 192 YEAIVLVKQFLSKKIKILESFKVL 212

The following BLAST results are available for this feature:

BLAST of Bhi05G000509 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q6TPK4\|CYT1_ACTDE	2.0e-06	40.54	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
sp\|Q84WT8\|CYT4_ARATH	5.0e-05	32.39	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]

BLAST of Bhi05G000509 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16500.1	2.8e-06	32.39	Cystatin/monellin superfamily protein	[more]

BLAST of Bhi05G000509 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0K3W6\|A0A0A0K3W6_CUCSA	9.6e-30	37.88	Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_7G222880 PE=3 SV...	[more]
tr\|A0A0A0LYF4\|A0A0A0LYF4_CUCSA	1.3e-29	39.70	Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_1G710160 PE=3 SV...	[more]
tr\|A0A1S3BHQ3\|A0A1S3BHQ3_CUCME	5.3e-28	40.20	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103489701 PE=3 SV=1	[more]
tr\|P94012\|P94012_CUCMA	8.2e-21	33.66	Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=PP1 PE=3 SV=1	[more]
tr\|Q7DMD5\|Q7DMD5_CUCMA	3.2e-17	28.00	Cysteine proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=cPC7 PE=...	[more]

BLAST of Bhi05G000509 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659743.1	2.0e-34	44.04	PREDICTED: uncharacterized protein LOC101206992 [Cucumis sativus]	[more]
KGN44208.1	1.5e-29	37.88	Phloem filament protein [Cucumis sativus]	[more]
XP_011660324.1	1.9e-29	39.70	PREDICTED: uncharacterized protein LOC105436367 [Cucumis sativus] >KGN66905.1 Ph...	[more]
XP_008447193.1	8.0e-28	40.20	PREDICTED: uncharacterized protein LOC103489701 [Cucumis melo]	[more]
XP_022989567.1	3.3e-21	33.33	uncharacterized protein LOC111486626 isoform X2 [Cucurbita maxima]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027214	Cystatin
IPR009994	PP1
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi05M000509	Bhi05M000509	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 129..219 e-value: 6.8E-14 score: 53.7
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 36..123 e-value: 8.8E-7 score: 30.7
None	No IPR available	PANTHER	PTHR11413:SF65	CYSTEINE PROTEINASE INHIBITOR 5	coord: 26..119
None	No IPR available	PANTHER	PTHR11413:SF65	CYSTEINE PROTEINASE INHIBITOR 5	coord: 128..211
None	No IPR available	PRODOM	PD001231		coord: 138..200 e-value: 2.0E-6 score: 123.0
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 43..112
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 131..207
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 134..209 e-value: 8.3E-17 score: 61.2
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 132..221 e-value: 2.05093E-5 score: 40.15
IPR009994	Phloem filament PP1	PFAM	PF07430	PP1	coord: 44..112 e-value: 2.7E-7 score: 30.4
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 128..211
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 26..119

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi05G000509	ClCG09G014700	Watermelon (Charleston Gray)	wcgwgoB044
Bhi05G000509	CmaCh11G015720	Cucurbita maxima (Rimu)	cmawgoB0116
Bhi05G000509	Lsi05G008350	Bottle gourd (USVL1VR-Ls)	lsiwgoB461
Bhi05G000509	Cla97C09G176950	Watermelon (97103) v2	wgowmbB273

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi05G000509	Cucurbita maxima (Rimu)	cmawgoB0649