Cucurbit Expression Atlas


Overview of this BioProject:

BioProjectPRJNA285071
Vlaspik and PI 197088 cucumbers inoculated with Pseudoperonospora cubensis (MSU-1) raw sequence reads. The approach of this study was to sequence and analyze the transcriptomes and small RNAomes of a susceptible (cv. Vlaspik) and resistant (PI 197088) cucumber in response to a time course of infection with downy mildew, Pseudoperonospora cubensis. The goal of this study was to identify differentially expressed genes between plant lines and time points that could be contributing to resistance. In parallel (from the same biological samples), small RNA was sequenced in order to identify miRNA loci in cucumber and P. cubensis. The goal was to correlate miRNA expression with changes in the transcriptome. In total, this work produced lists of candidate resistance-associated genes and predicted miRNA loci and targets in cucumber and P. cubensis.

Properties this BioBroject:

Property NameValue
Data typeTranscriptome or Gene expression
Sample scopeMulti-species
MaterialTranscriptome
CaptureRandomSurvey
Experimental_design

BioSamples of this BioProject:

BioSampleOrganismDescription
PI88_1dpiCucumber4 weeks old leaf infected with downy mildew at 1 day post infection ; cultivar : PI 197088; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 1 dpi;
PI88_2dpiCucumber4 weeks old leaf infected with downy mildew at 2 day post infection ; cultivar : PI 197088; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 2 dpi;
PI88_3dpiCucumber4 weeks old leaf infected with downy mildew at 3 day post infection ; cultivar : PI 197088; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 3 dpi;
PI88_4dpiCucumber4 weeks old leaf infected with downy mildew at 4 day post infection ; cultivar : PI 197088; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 4 dpi;
PI88_6dpiCucumber4 weeks old leaf infected with downy mildew at 6 day post infection ; cultivar : PI 197088; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 6 dpi;
PI88_MockCucumberMock ; cultivar : PI 197088; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : Mock;
Vlas_1dpiCucumber4 weeks old leaf infected with downy mildew at 1 day post infection ; cultivar : Vlaspik; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 1 dpi;
Vlas_2dpiCucumber4 weeks old leaf infected with downy mildew at 2 day post infection ; cultivar : Vlaspik; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 2 dpi;
Vlas_3dpiCucumber4 weeks old leaf infected with downy mildew at 3 day post infection ; cultivar : Vlaspik; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 3 dpi;
Vlas_4dpiCucumber4 weeks old leaf infected with downy mildew at 4 day post infection ; cultivar : Vlaspik; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 4 dpi;
Vlas_6dpiCucumber4 weeks old leaf infected with downy mildew at 6 day post infection ; cultivar : Vlaspik; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : 6 dpi;
Vlas_MockCucumberMock ; cultivar : Vlaspik; age : 4 weeks; tissue : leaf; disease : downy mildew; disease_stage : Mock;