Cucurbit Expression Atlas


Overview of this BioProject:

BioProjectPRJNA292785
Next Generation Sequencing Facilitates Quantitative Analysis of Physcion and Chrysophanol on cucumber powdery mildew Transcriptomes (cucumber). We generated 17.6 Gb of high-quality sequencing data (~2 Gb per sample) and catalogued the expression profiles of 12,293 annotated cucumber genes in each sample. The analysis showed differences of transcriptomes between physcion and chrysophanol alone treatment and combination treatment as well as revealed that the combination treatment the most defence-related genes induced expression changes. We identified numerous differentially expressed genes that exhibited distinct expression patterns, and defence-related genes that were highly differentiated between the physcion and chrysophanol alone treatment and combination treatment. These genes have known or potential roles in defense response and were enriched in functional gene categories potentially responsible for cucumber resistance. Therefore, they are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to defense response. Overall design: cucumber leaves mRNA profiles of physcion and chrysophanol alone treatment and combination treatment were generated by deep sequencing, in two or triplicate, using Illumina HiSeq2000.

Properties this BioBroject:

Property NameValue
Data typeTranscriptome or Gene expression
Sample scopeMultiisolate
MaterialTranscriptome
CaptureRandomSurvey
Experimental_designsample CT Ch Ph Ph_Ch # CT: control # Ch: treated with chrysophanol # Ph: treated with physcion # Ph_Ch: treated with both physcion and chrysophanol

BioSamples of this BioProject:

BioSampleOrganismDescription
CTCucumbercucumber leaves without treatment as control ; cultivar : Changchunmici; tissue : leaves; treatment : contorl;
ChCucumbercucumber leaves treated with chrysophanol ; cultivar : Changchunmici; tissue : leaves; treatment : chrysophanol;
PhCucumbercucumber leaves treated with physcion ; cultivar : Changchunmici; tissue : leaves; treatment : physcion;
Ph_ChCucumbercucumber leaves treated with both physcion and chrysophanol ; cultivar : Changchunmici; tissue : leaves; treatment : physcion and chrysophanol;