WMU41466 (transcribed_cluster) Watermelon (97103) v1

Overview
NameWMU41466
Typetranscribed_cluster
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionUnknown protein
LocationChr7 : 14827087 .. 14827614 (-)
Sequence length536
Genome Viewer
Sequences
The following sequences are available for this feature:

transcribed_cluster sequence

GCTCTCGATCTCCTCCTCGAGCTGCCTAGCTGACCGTTTCTCCGTTCCCTTGAAGATCATATGCTCGAGGAAATGAGCAGTGCCATTAGTCTCCTCGGTCTCGAACCTCGACCCGGCATCAATCCAAAACTCCAACCGTCGCAGTCCGAGCGGCAAGGTTTGACTCAGTGGCCACCCGGAGGCCATTGGGGAGAGTAGTAACGCGAGTTTCAGGCGCGGAGAGAATCCGGGGTATGGTCAGTGATGGTCGGGTGAGGCGAGCCGTATTTGAGAAACCTAGGGTCGGGATTCTCGAGCTGTTTGAGCTTGGATTTCACAGCCTCCGCGAGGCGATCGTAGATCATGGCATTGGGCGGCGGAGGAGAGGGAGGTGGAGAAGAAGAGGCTACCGCCGGGGAAGTGGAAGCCGATCTAATAGCCTGGTGGAAAGGAGGCAAAAGATCGCCGGTGAGAGGAGCGAGCGAGGGTGAGAAGACGCTTGATCGCCATGATTGATTTTGGGCCCTTGCTCCCTAGGGTTTACAGCGGCTACCCCC
Homology
[Close]
BLAST of WMU41466 vs. TAIR10
Match: AT3G02090.2 (AT3G02090.2 Insulinase (Peptidase family M16) protein)

HSP 1 Score: 78.2 bits (191), Expect = 5.8e-15
Identity = 40/64 (62.50%), Postives = 44/64 (68.75%), Query Frame = -3 

Query: 4   TLPLGLR------------RLEFWIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLE 63
           TLP GLR             +  WIDAGSRFE++ETNGTAHFLEHMIFKGT++R+ R LE
Sbjct: 101 TLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRTVRALE 160

Query: 64  EEIE 123
           EEIE
Sbjct: 161 EEIE 164

Query: 124  160
           
Sbjct: 221  164


HSP 2 Score: 62.8 bits (151), Expect = 2.5e-10
Identity = 31/33 (93.94%), Postives = 31/33 (93.94%), Query Frame = -2 

Query: 128 ILSAPETRVTTLPNGLRVATESNLAARTATVGV 187
           ILSAPETRVTTLPNGLRVATESNL+A+TATVGV
Sbjct: 91  ILSAPETRVTTLPNGLRVATESNLSAKTATVGV 123

Query: 188  227
           
Sbjct: 151  123


HSP 3 Score: 55.8 bits (133), Expect = 3.1e-08
Identity = 23/37 (62.16%), Postives = 26/37 (70.27%), Query Frame = -1 

Query: 234 MIYDRLAEAVKSKLKQLENPDPRFLKYGSPHPTITDH 293
           M YD  AE +K+K+K+LENPD RFLKY SPHP +  H
Sbjct: 52  MPYDHAAEIIKNKIKKLENPDKRFLKYASPHPILASH 88

Query: 294  345
           
Sbjct: 112  88

[Close]
BLAST of WMU41466 vs. Swiss-Prot
Match: MPPB_ARATH (Probable mitochondrial-processing peptidase subunit beta, mitochondrial OS=Arabidopsis thaliana GN=At3g02090 PE=2 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 1.0e-13
Identity = 40/64 (62.50%), Postives = 44/64 (68.75%), Query Frame = -3 

Query: 4   TLPLGLR------------RLEFWIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLE 63
           TLP GLR             +  WIDAGSRFE++ETNGTAHFLEHMIFKGT++R+ R LE
Sbjct: 101 TLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGTDRRTVRALE 160

Query: 64  EEIE 123
           EEIE
Sbjct: 161 EEIE 164

Query: 124  160
           
Sbjct: 221  164


HSP 2 Score: 62.8 bits (151), Expect = 4.5e-09
Identity = 31/33 (93.94%), Postives = 31/33 (93.94%), Query Frame = -2 

Query: 128 ILSAPETRVTTLPNGLRVATESNLAARTATVGV 187
           ILSAPETRVTTLPNGLRVATESNL+A+TATVGV
Sbjct: 91  ILSAPETRVTTLPNGLRVATESNLSAKTATVGV 123

Query: 188  227
           
Sbjct: 151  123


HSP 3 Score: 55.8 bits (133), Expect = 5.4e-07
Identity = 23/37 (62.16%), Postives = 26/37 (70.27%), Query Frame = -1 

Query: 234 MIYDRLAEAVKSKLKQLENPDPRFLKYGSPHPTITDH 293
           M YD  AE +K+K+K+LENPD RFLKY SPHP +  H
Sbjct: 52  MPYDHAAEIIKNKIKKLENPDKRFLKYASPHPILASH 88

Query: 294  345
           
Sbjct: 112  88

[Close]
BLAST of WMU41466 vs. Swiss-Prot
Match: MPPB_NEUCR (Mitochondrial-processing peptidase subunit beta OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=pep PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.3e-12
Identity = 36/57 (63.16%), Postives = 42/57 (73.68%), Query Frame = -3 

Query: 1   PLSQTLPLGLRRLEFWIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLEEEIES 60
           P +QT  +G+     WIDAGSR ET+ETNGTAHFLEH+ FKGT KR+ +QLE EIE+
Sbjct: 57  PYAQTSTVGM-----WIDAGSRAETDETNGTAHFLEHLAFKGTTKRTQQQLELEIEN 108

Query: 61   120
           
Sbjct: 117  108

Query: 121  172
           
Sbjct: 177  108


HSP 2 Score: 32.3 bits (72), Expect = 6.4e+00
Identity = 17/41 (41.46%), Postives = 24/41 (58.54%), Query Frame = -2 

Query: 128 RPSLTIPRILSAPETRVTTLPNGLRVATESNLAARTATVGV 187
           R  L  P   +  +T+ TTL NGL VA++ +  A+T+TVG+
Sbjct: 26  RRGLATPHSGTGIKTQTTTLKNGLTVASQYSPYAQTSTVGM 66

Query: 188  247
           
Sbjct: 86   66

Query: 248  251
           
Sbjct: 146  66

[Close]
BLAST of WMU41466 vs. Swiss-Prot
Match: MPPB_LENED (Mitochondrial-processing peptidase subunit beta OS=Lentinula edodes GN=mppB PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.1e-11
Identity = 34/57 (59.65%), Postives = 40/57 (70.18%), Query Frame = -3 

Query: 1   PLSQTLPLGLRRLEFWIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLEEEIES 60
           P +QT  +G+     WIDAGSR ET++TNGTAHFLEHM FKGT +RS   LE E+E+
Sbjct: 46  PHAQTATVGV-----WIDAGSRAETDKTNGTAHFLEHMAFKGTGRRSQHALELEVEN 97

Query: 61   120
           
Sbjct: 106  97

Query: 121  172
           
Sbjct: 166  97


HSP 2 Score: 37.7 bits (86), Expect = 1.5e-01
Identity = 19/29 (65.52%), Postives = 20/29 (68.97%), Query Frame = -2 

Query: 128 PETRVTTLPNGLRVATESNLAARTATVGV 187
           P T ++TL NGL VATES   A+TATVGV
Sbjct: 27  PFTEISTLSNGLTVATESQPHAQTATVGV 55

Query: 188  215
           
Sbjct: 87   55

[Close]
BLAST of WMU41466 vs. Swiss-Prot
Match: MPPB_BOVIN (Mitochondrial-processing peptidase subunit beta OS=Bos taurus GN=PMPCB PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 31/42 (73.81%), Postives = 33/42 (78.57%), Query Frame = -3 

Query: 1   WIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLEEEIES 60
           WIDAGSR+E E+ NGTAHFLEHM FKGT+KRS   LE EIE+
Sbjct: 85  WIDAGSRYENEKNNGTAHFLEHMAFKGTKKRSQLDLELEIEN 126

Query: 61   120
           
Sbjct: 145  126

Query: 121  127
           
Sbjct: 205  126


HSP 2 Score: 37.7 bits (86), Expect = 1.5e-01
Identity = 23/47 (48.94%), Postives = 29/47 (61.70%), Query Frame = -2 

Query: 128 NTARLTRPSLTIPRILSAPETRVTTLPNGLRVATESNLAARTATVGV 187
           N  R T+ +  +  +L+ PETRVT L NGLRVA+E +  A T TVG+
Sbjct: 41  NRLRSTQAAAQV--VLNVPETRVTCLENGLRVASEDSGLA-TCTVGL 84

Query: 188  247
           
Sbjct: 101  84

Query: 248  269
           
Sbjct: 161  84

[Close]
BLAST of WMU41466 vs. Swiss-Prot
Match: MPPB_RAT (Mitochondrial-processing peptidase subunit beta OS=Rattus norvegicus GN=Pmpcb PE=1 SV=3)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-10
Identity = 31/42 (73.81%), Postives = 33/42 (78.57%), Query Frame = -3 

Query: 1   WIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLEEEIES 60
           WIDAGSR+E E+ NGTAHFLEHM FKGT+KRS   LE EIE+
Sbjct: 84  WIDAGSRYENEKNNGTAHFLEHMAFKGTKKRSQLDLELEIEN 125

Query: 61   120
           
Sbjct: 144  125

Query: 121  127
           
Sbjct: 204  125


HSP 2 Score: 36.6 bits (83), Expect = 3.4e-01
Identity = 19/33 (57.58%), Postives = 22/33 (66.67%), Query Frame = -2 

Query: 128 ILSAPETRVTTLPNGLRVATESNLAARTATVGV 187
           +L+ PET+VT L NGLRVA+E N    T TVG+
Sbjct: 52  VLNVPETQVTCLENGLRVASE-NSGISTCTVGL 83

Query: 188  227
           
Sbjct: 112  83

[Close]
BLAST of WMU41466 vs. NCBI nr
Match: gi|661902917|ref|NP_001284394.1| (probable mitochondrial-processing peptidase subunit beta [Cucumis melo])

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-14
Identity = 44/66 (66.67%), Postives = 45/66 (68.18%), Query Frame = -1 

Query: 231 FHQAIRXXXXXXXXXXXXXXXXXXXXNAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP 290
           F QA+R                    NAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP
Sbjct: 21  FSQAVRSASTFPAVASSSPLPSPPPPNAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP 80

Query: 291 TITDHT 350
           TITDHT
Sbjct: 81  TITDHT 86

Query: 351  410
           
Sbjct: 141  86

Query: 411  429
           
Sbjct: 201  86

[Close]
BLAST of WMU41466 vs. NCBI nr
Match: gi|449438845|ref|XP_004137198.1| (PREDICTED: probable mitochondrial-processing peptidase subunit beta [Cucumis sativus])

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-14
Identity = 44/66 (66.67%), Postives = 45/66 (68.18%), Query Frame = -1 

Query: 231 FHQAIRXXXXXXXXXXXXXXXXXXXXNAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP 290
           F QA+R                    NAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP
Sbjct: 21  FSQAVRSASTSPAVASSSPPPSPPPPNAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP 80

Query: 291 TITDHT 350
           TITDHT
Sbjct: 81  TITDHT 86

Query: 351  410
           
Sbjct: 141  86

Query: 411  429
           
Sbjct: 201  86

[Close]
BLAST of WMU41466 vs. NCBI nr
Match: gi|659101564|ref|XP_008451674.1| (PREDICTED: probable mitochondrial-processing peptidase subunit beta isoform X2 [Cucumis melo])

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-14
Identity = 44/66 (66.67%), Postives = 45/66 (68.18%), Query Frame = -1 

Query: 231 FHQAIRXXXXXXXXXXXXXXXXXXXXNAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP 290
           F QA+R                    NAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP
Sbjct: 21  FSQAVRSASTFPAVASSSPLPSPPPPNAMIYDRLAEAVKSKLKQLENPDPRFLKYGSPHP 80

Query: 291 TITDHT 350
           TITDHT
Sbjct: 81  TITDHT 86

Query: 351  410
           
Sbjct: 141  86

Query: 411  429
           
Sbjct: 201  86

[Close]
BLAST of WMU41466 vs. NCBI nr
Match: gi|823167118|ref|XP_012483507.1| (PREDICTED: probable mitochondrial-processing peptidase subunit beta isoform X2 [Gossypium raimondii])

HSP 1 Score: 86.7 bits (213), Expect = 4.6e-14
Identity = 46/65 (70.77%), Postives = 49/65 (75.38%), Query Frame = -3 

Query: 1   TLPLGLR------------RLEFWIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLE 60
           TLP GLR             +  WIDAGSRFETEETNGTAHFLEHMIFKGTEKRSAR+LE
Sbjct: 98  TLPNGLRVATESTLSSHTATVGVWIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARELE 157

Query: 61  EEIES 120
           EEIE+
Sbjct: 158 EEIEN 162

Query: 121  160
           
Sbjct: 218  162

[Close]
BLAST of WMU41466 vs. NCBI nr
Match: gi|763766227|gb|KJB33442.1| (hypothetical protein B456_006G011200 [Gossypium raimondii])

HSP 1 Score: 86.7 bits (213), Expect = 4.6e-14
Identity = 46/65 (70.77%), Postives = 49/65 (75.38%), Query Frame = -3 

Query: 1   TLPLGLR------------RLEFWIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARQLE 60
           TLP GLR             +  WIDAGSRFETEETNGTAHFLEHMIFKGTEKRSAR+LE
Sbjct: 98  TLPNGLRVATESTLSSHTATVGVWIDAGSRFETEETNGTAHFLEHMIFKGTEKRSARELE 157

Query: 61  EEIES 120
           EEIE+
Sbjct: 158 EEIEN 162

Query: 121  160
           
Sbjct: 218  162

The following BLAST results are available for this feature:
BLAST of WMU41466 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G02090.25.8e-1562.50 Insulinase (Peptidase family M16) protein[more]
BLAST of WMU41466 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MPPB_ARATH1.0e-1362.50Probable mitochondrial-processing peptidase subunit beta, mitochondrial OS=Arabi... [more]
MPPB_NEUCR3.3e-1263.16Mitochondrial-processing peptidase subunit beta OS=Neurospora crassa (strain ATC... [more]
MPPB_LENED8.1e-1159.65Mitochondrial-processing peptidase subunit beta OS=Lentinula edodes GN=mppB PE=3... [more]
MPPB_BOVIN1.1e-1073.81Mitochondrial-processing peptidase subunit beta OS=Bos taurus GN=PMPCB PE=2 SV=1[more]
MPPB_RAT1.1e-1073.81Mitochondrial-processing peptidase subunit beta OS=Rattus norvegicus GN=Pmpcb PE... [more]
BLAST of WMU41466 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|661902917|ref|NP_001284394.1|1.2e-1466.67probable mitochondrial-processing peptidase subunit beta [Cucumis melo][more]
gi|449438845|ref|XP_004137198.1|1.2e-1466.67PREDICTED: probable mitochondrial-processing peptidase subunit beta [Cucumis sat... [more]
gi|659101564|ref|XP_008451674.1|1.2e-1466.67PREDICTED: probable mitochondrial-processing peptidase subunit beta isoform X2 [... [more]
gi|823167118|ref|XP_012483507.1|4.6e-1470.77PREDICTED: probable mitochondrial-processing peptidase subunit beta isoform X2 [... [more]
gi|763766227|gb|KJB33442.1|4.6e-1470.77hypothetical protein B456_006G011200 [Gossypium raimondii][more]
Annotated Terms
The following terms have been associated with this transcribed_cluster:
Vocabulary: INTERPRO
TermDefinition
IPR001431Pept_M16_Zn_BS
IPR011237Peptidase M16 domain
IPR011249Metalloenz_LuxS/M16
IPR011765Pept_M16_N
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0046872metal ion binding
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Annotation
GO Assignments
This transcribed_cluster is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0003824 catalytic activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity
Relationships

This transcribed_cluster is associated with the following gene feature(s):

Feature NameUnique NameType
Cla001475Cla001475gene


The following EST feature(s) are a part of this transcribed_cluster:

Feature NameUnique NameType
S1_0071988S1_0071988EST
S1_0084513S1_0084513EST
S1_0104269S1_0104269EST
S2_0085161S2_0085161EST
S3_0089781S3_0089781EST
S4_0178111S4_0178111EST


InterPro
Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001431Peptidase M16, zinc-binding sitePROSITEPS00143INSULINASEcoord: 126..149
scor
IPR011237Peptidase M16 domainGENE3DG3DSA:3.30.830.10coord: 119..163
score: 1.1
IPR011249Metalloenzyme, LuxS/M16 peptidase-likeunknownSSF63411LuxS/MPP-like metallohydrolasecoord: 119..163
score: 2.8
IPR011765Peptidase M16, N-terminalPFAMPF00675Peptidase_M16coord: 121..162
score: 7.7
NoneNo IPR availablePANTHERPTHR11851METALLOPROTEASEcoord: 19..163
score: 5.2
NoneNo IPR availablePANTHERPTHR11851:SF149MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT BETAcoord: 19..163
score: 5.2