MELO3C025954T1 (mRNA) Melon (DHL92) v3.5.1

NameMELO3C025954T1
TypemRNA
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionCortactin-binding protein 2
Locationchr11 : 13675821 .. 13676506 (-)
Sequence length627
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTCGTCAAGGAACTTGAGGCAGAAGAGAAGAAGGCTGAAGCAAAAGAGAAGAAGAAAGCTGAAGAGGAAAAGAGAAAAGAAGAAGAAAGTTTAAGAAGGAAAAAGGAAGAGAAAAAAGCCCTGCGAGAAGAAAGCGCTAAGCGAGAAGAAGAGAGGAGGAAGAAAGAAAGAGAAAAGAAAAAGGTCACAAGGAGGACGCCCATCTCTGCTAAGTGTTTAAAGAGAAAAAAGAAGAATGTTGAATCTTTGGTGGAGACCAAAACCAAATTGGCAGAGATCCAAACCGCTAAGAAAAAGAAAAAAGAGACACTCATGGACCAAAGTGAGCAAGTGGAAAGTGTAGTAGCAAGAAACAAGACTACCATGAGGTTGTGGCTGAAGAGTTTAGTCAAGAAATTGAGAGGATGAGTCCTTTGAAGCCAAGGCTAAGGCCTGAAGAACTAGGGCTCATAAGTGTCGTGCAACCGAAGAAAGGCAAGAATAAGACGGGGGCATTAAGGCACTTAGGTCGTTTGCTCAATTTGCTCTACGAACCCATTAAGGCACTTAAGTCGGAGAAGATTTTCATGGGTGAGACTAAGGTTAGATTGGACATTGTTAATATGTTTTATGCGAGGAAGTATCATCCGGATGATTCATACGCGATAGTGGAAGGGGAGAAGATGCCTTTTACCACATAA

mRNA sequence

ATGAAATTCGTCAAGGAACTTGAGGCAGAAGAGAAGAAGGCTGAAGCAAAAGAGAAGAAGAAAGCTGAAGAGGAAAAGAGAAAAGAAGAAGAAAGTTTAAGAAGGAAAAAGGAAGAGAAAAAAGCCCTGCGAGAAGAAAGCGCTAAGCGAGAAGAAGAGAGGAGGAAGAAAGAAAGAGAAAAGAAAAAGGTCACAAGGAGGACGCCCATCTCTGCTAAGTGTTTAAAGAGAAAAAAGAAGAATGTTGAATCTTTGGTGGAGACCAAAACCAAATTGGCAGAGATCCAAACCGCTAAGAAAAAGAAAAAAGAGACACTCATGGACCAAAAGTTTAGTCAAGAAATTGAGAGGATGAGTCCTTTGAAGCCAAGGCTAAGGCCTGAAGAACTAGGGCTCATAAGTGTCGTGCAACCGAAGAAAGGCAAGAATAAGACGGGGGCATTAAGGCACTTAGGTCGTTTGCTCAATTTGCTCTACGAACCCATTAAGGCACTTAAGTCGGAGAAGATTTTCATGGGTGAGACTAAGGTTAGATTGGACATTGTTAATATGTTTTATGCGAGGAAGTATCATCCGGATGATTCATACGCGATAGTGGAAGGGGAGAAGATGCCTTTTACCACATAA

Coding sequence (CDS)

ATGAAATTCGTCAAGGAACTTGAGGCAGAAGAGAAGAAGGCTGAAGCAAAAGAGAAGAAGAAAGCTGAAGAGGAAAAGAGAAAAGAAGAAGAAAGTTTAAGAAGGAAAAAGGAAGAGAAAAAAGCCCTGCGAGAAGAAAGCGCTAAGCGAGAAGAAGAGAGGAGGAAGAAAGAAAGAGAAAAGAAAAAGGTCACAAGGAGGACGCCCATCTCTGCTAAGTGTTTAAAGAGAAAAAAGAAGAATGTTGAATCTTTGGTGGAGACCAAAACCAAATTGGCAGAGATCCAAACCGCTAAGAAAAAGAAAAAAGAGACACTCATGGACCAAAAGTTTAGTCAAGAAATTGAGAGGATGAGTCCTTTGAAGCCAAGGCTAAGGCCTGAAGAACTAGGGCTCATAAGTGTCGTGCAACCGAAGAAAGGCAAGAATAAGACGGGGGCATTAAGGCACTTAGGTCGTTTGCTCAATTTGCTCTACGAACCCATTAAGGCACTTAAGTCGGAGAAGATTTTCATGGGTGAGACTAAGGTTAGATTGGACATTGTTAATATGTTTTATGCGAGGAAGTATCATCCGGATGATTCATACGCGATAGTGGAAGGGGAGAAGATGCCTTTTACCACATAA

Protein sequence

MKFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKPRLRPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEPIKALKSEKIFMGETKVRLDIVNMFYARKYHPDDSYAIVEGEKMPFTT*
BLAST of MELO3C025954T1 vs. Swiss-Prot
Match: CALD1_CHICK (Caldesmon OS=Gallus gallus GN=CALD1 PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 2.5e-11
Identity = 39/119 (32.77%), Postives = 63/119 (52.94%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREK 61
           K  +E +A E++A+A+E++KA EE+ K EE  +  +E  KA +E  A  E ER K E EK
Sbjct: 322 KAEEERKAAEERAKAEEERKAAEERAKAEEERKAAEERAKAEKERKAAEERERAKAEEEK 381

Query: 62  KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSP 121
           +    +  + A+ LK KKK  E   + +   A +   +++ KE  ++ K     E++ P
Sbjct: 382 RAAEEKARLEAEKLKEKKKMEEKKAQEEKAQANLLRKQEEDKEAKVEAKKESLPEKLQP 440


HSP 2 Score: 66.2 bits (160), Expect = 4.7e-10
Identity = 51/149 (34.23%), Postives = 77/149 (51.68%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALRE--ESAKREEERR-KKEREK 64
           + L+AEE+K  A+EK+KAEEEK+  EE  R K EE+K   E  E AK EEER+  +ERE+
Sbjct: 261 ERLKAEEEKKAAEEKQKAEEEKKAAEERERAKAEEEKRAAEERERAKAEEERKAAEERER 320

Query: 65  KKVTRRTPISAKCLK----RKKKNVESLVETKTKLAE--IQTAKKKKKETLMDQKFSQEI 124
            K       + +  K    RK     +  E + K AE   +  K++K     ++  ++E 
Sbjct: 321 AKAEEERKAAEERAKAEEERKAAEERAKAEEERKAAEERAKAEKERKAAEERERAKAEEE 380

Query: 125 ERMSPLKPRLRPEELGLISVVQPKKGKNK 145
           +R +  K RL  E+L     ++ KK + +
Sbjct: 381 KRAAEEKARLEAEKLKEKKKMEEKKAQEE 409


HSP 3 Score: 63.5 bits (153), Expect = 3.1e-09
Identity = 47/141 (33.33%), Postives = 73/141 (51.77%), Query Frame = 1

Query: 8   EAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEER-----RKKEREKK 67
           E E++ AE +E+ KAEEE++  EE  R K EE++   EE AK EEER     R K  E++
Sbjct: 294 EEEKRAAEERERAKAEEERKAAEERERAKAEEERKAAEERAKAEEERKAAEERAKAEEER 353

Query: 68  KVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQT---AKKKKKETLMDQKFSQEIERMS 127
           K       + K  K  ++   +  E + + AE +    A+K K++  M++K +QE +  +
Sbjct: 354 KAAEERAKAEKERKAAEERERAKAEEEKRAAEEKARLEAEKLKEKKKMEEKKAQEEKAQA 413

Query: 128 PLKPRLRPEELGLISVVQPKK 141
            L   LR +E    + V+ KK
Sbjct: 414 NL---LRKQEEDKEAKVEAKK 431


HSP 4 Score: 52.8 bits (125), Expect = 5.4e-06
Identity = 54/198 (27.27%), Postives = 84/198 (42.42%), Query Frame = 1

Query: 6   ELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKVT 65
           E E E+ +AE KE+ KAEEEK+  EE       ++KA  E+ A  E ER K E EK+   
Sbjct: 249 EKEREKLEAEEKERLKAEEEKKAAEE-------KQKAEEEKKAAEERERAKAEEEKRAAE 308

Query: 66  RRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKPRL 125
            R    A+  ++  +  E     + + A  + AK +++    +++   E ER +  +   
Sbjct: 309 ERERAKAEEERKAAEERERAKAEEERKAAEERAKAEEERKAAEERAKAEEERKAAEERAK 368

Query: 126 RPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEPIKALKSEKIFMGETKVRLDIVNMF 185
             +E       +  K + +  A     RL        + LK EK  M E K + +     
Sbjct: 369 AEKERKAAEERERAKAEEEKRAAEEKARL------EAEKLK-EKKKMEEKKAQEEKAQAN 428

Query: 186 YARKYHPDDSYAIVEGEK 204
             RK   +D  A VE +K
Sbjct: 429 LLRK-QEEDKEAKVEAKK 431


HSP 5 Score: 51.6 bits (122), Expect = 1.2e-05
Identity = 42/143 (29.37%), Postives = 67/143 (46.85%), Query Frame = 1

Query: 7   LEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALR-EESAKREEERRKKEREKKKVT 66
           LE E+   +A +K+K E EK +E    + + EEK+ L+ EE  K  EE++K E EKK   
Sbjct: 231 LEGEQSITDAADKEKEEAEKERE----KLEAEEKERLKAEEEKKAAEEKQKAEEEKKAAE 290

Query: 67  RRTPISAKCLKRKKKNVE-SLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKPR 126
            R    A+  KR  +  E +  E + K AE +   K ++E    ++ ++  E     + R
Sbjct: 291 ERERAKAEEEKRAAEERERAKAEEERKAAEERERAKAEEERKAAEERAKAEEERKAAEER 350

Query: 127 LRPEELGLISVVQPKKGKNKTGA 148
            + EE    +  + K  K +  A
Sbjct: 351 AKAEEERKAAEERAKAEKERKAA 369


HSP 6 Score: 50.4 bits (119), Expect = 2.7e-05
Identity = 33/114 (28.95%), Postives = 60/114 (52.63%), Query Frame = 1

Query: 8   EAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKVTRR 67
           E E K AE +E+ KAEEEKR  EE  R + E+ K    E  K EE++ ++E+ +  + R+
Sbjct: 363 EKERKAAEERERAKAEEEKRAAEEKARLEAEKLK----EKKKMEEKKAQEEKAQANLLRK 422

Query: 68  TPISAKC-LKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSP 121
                +  ++ KK+++   ++  +K  +++  K K+K    + K   + +R  P
Sbjct: 423 QEEDKEAKVEAKKESLPEKLQPTSKKDQVKDNKDKEKAPKEEMKSVWDRKRGVP 472


HSP 7 Score: 42.4 bits (98), Expect = 7.3e-03
Identity = 28/93 (30.11%), Postives = 51/93 (54.84%), Query Frame = 1

Query: 1   MKFVKELEAEEKKAEAKEKKKAEE----EKRKEEESLRRKKEEKKALREESAKREEERRK 60
           +K     EA  +K + K+++ A E    +KR+EE     ++EE+K  +EE+ ++  E  +
Sbjct: 500 LKGAANAEAGSEKLKEKQQEAAVELDELKKRREERRKILEEEEQKKKQEEAERKIREEEE 559

Query: 61  KEREKKKVTRRTPISAKCLKRKKKNVESLVETK 90
           K+R K+++ RR   +A+  KR+K   + + E K
Sbjct: 560 KKRMKEEIERRRAEAAE--KRQKVPEDGVSEEK 590


HSP 8 Score: 37.7 bits (86), Expect = 1.8e-01
Identity = 28/116 (24.14%), Postives = 49/116 (42.24%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREK 61
           K ++E E ++K+ EA+ K + EEEK++ +E + R++ E             E+R+K  E 
Sbjct: 536 KILEEEEQKKKQEEAERKIREEEEKKRMKEEIERRRAE-----------AAEKRQKVPED 595

Query: 62  KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIER 118
                + P      K     +E   E   K A+ ++  K    T +  K    +E+
Sbjct: 596 GVSEEKKPFKCFSPKGSSLKIEERAEFLNKSAQ-KSGMKPAHTTAVVSKIDSRLEQ 639


HSP 9 Score: 37.4 bits (85), Expect = 2.4e-01
Identity = 28/119 (23.53%), Postives = 53/119 (44.54%), Query Frame = 1

Query: 4   VKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKER---E 63
           +++ E  +   E  EK +   +    EE  +R  +++ AL E  A+REE R+K+ +   E
Sbjct: 50  LRQKEEGDVSGEVTEKSEVNAQNSVAEEETKRSTDDEAALLERLARREERRQKRLQEALE 109

Query: 64  KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMS 120
           ++K    T          ++ V ++ E +       T K++K ET   +   +E E ++
Sbjct: 110 RQKEFDPTITDGSLSVPSRREVNNVEENEI------TGKEEKVETRQGRCEIEETETVT 162

BLAST of MELO3C025954T1 vs. Swiss-Prot
Match: NST1_VANPO (Stress response protein NST1 OS=Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294) GN=NST1 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.3e-11
Identity = 48/142 (33.80%), Postives = 80/142 (56.34%), Query Frame = 1

Query: 1   MKFVKELEAEE-KKAEAKEKKKAEEEKRKE---------EESLRRKKEEKKALREESAKR 60
           MK ++ELEAEE KK E +EKK+ + EK KE         EE  ++K+EE + L++E+ +R
Sbjct: 704 MKLLEELEAEESKKKEKEEKKQRKREKEKEKKRLLQVAKEEERKKKEEEAERLKKEAEER 763

Query: 61  EEERRKKEREKKKVTRRTPISAKCLK-RKKKNVESLVETKTKLAEIQTAKK--KKKETLM 120
           E ERR+ +R+K +  ++     K  K  +++  E   E + K+ E Q  KK  ++K+  +
Sbjct: 764 EMERRETQRKKVEAAKKKKDEEKKRKLEEQRKREEEQERQRKIKEEQKRKKDEERKQKEL 823

Query: 121 DQKFSQEIERMSPLKPRLRPEE 130
           ++K   E +R+   + R + EE
Sbjct: 824 EKKKKDEEKRIQEEEKRKKEEE 845


HSP 2 Score: 65.9 bits (159), Expect = 6.2e-10
Identity = 35/100 (35.00%), Postives = 59/100 (59.00%), Query Frame = 1

Query: 8   EAEEKKAEAKEKKKAEEEKRKEEESLRRKKE---EKKALREESAKREEERRKKEREKKKV 67
           E + KK EA +KKK EE+KRK EE  +R++E   ++K   E+  K++EER++KE EKKK 
Sbjct: 769 ETQRKKVEAAKKKKDEEKKRKLEEQRKREEEQERQRKIKEEQKRKKDEERKQKELEKKKK 828

Query: 68  TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKE 105
                I  +  ++K++  E     + K  +++  ++K+ E
Sbjct: 829 DEEKRIQEEEKRKKEEEAEQKTLNEKKRLDVEIMEQKRLE 868


HSP 3 Score: 64.3 bits (155), Expect = 1.8e-09
Identity = 42/116 (36.21%), Postives = 69/116 (59.48%), Query Frame = 1

Query: 5   KELEAE--EKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKK 64
           KE EAE  +K+AE +E ++ E +++K E + ++K EEKK   EE  KREEE+ ++ + K+
Sbjct: 749 KEEEAERLKKEAEEREMERRETQRKKVEAAKKKKDEEKKRKLEEQRKREEEQERQRKIKE 808

Query: 65  KVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERM 119
           +  R+     K  +RK+K +E   + K +   IQ  +K+KKE   +QK   E +R+
Sbjct: 809 EQKRK-----KDEERKQKELEK--KKKDEEKRIQEEEKRKKEEEAEQKTLNEKKRL 857


HSP 4 Score: 28.1 bits (61), Expect = 1.4e+02
Identity = 27/127 (21.26%), Postives = 47/127 (37.01%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKK----AEEEKRKEEESLRRKKEEKKALREESAKREEERRKKERE 64
           K+ + EEK+ + +EK+K    AE++   E++ L  +  E+K L EE  + +         
Sbjct: 825 KKKKDEEKRIQEEEKRKKEEEAEQKTLNEKKRLDVEIMEQKRLEEEEKRLKSTASTSNVP 884

Query: 65  KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSP 124
             ++ + TP            +  ++   T    I T+       L     S     M+P
Sbjct: 885 YAELLKMTPSLQPVSGNINDELFGMINAATSSQSIPTSSSHLNHLLQQNDASH--FNMTP 944

Query: 125 LKPRLRP 128
               L P
Sbjct: 945 RHDLLNP 949

BLAST of MELO3C025954T1 vs. Swiss-Prot
Match: NST1_KLULA (Stress response protein NST1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) GN=NST1 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-10
Identity = 48/131 (36.64%), Postives = 75/131 (57.25%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEE---RRKKE 61
           + + ELEAEE + + KEKKK    K+KE+E  + KK +++  +EE  KR+EE   R KKE
Sbjct: 688 RLLMELEAEENQKKEKEKKKL---KKKEKE--KEKKRQQQLAKEEEKKRQEEEEIRLKKE 747

Query: 62  REKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERM 121
            E+K++ RR     K  + K+KN E   + K KLAE Q  +++++E +  +K  Q+ +R 
Sbjct: 748 AEEKEIARREAQRKKVEEAKRKNDE---KRKKKLAE-QRRREEEQERIRKEKEEQKRQRE 807

Query: 122 SPLKPRLRPEE 130
              K +   +E
Sbjct: 808 EEQKQKKMEKE 809


HSP 2 Score: 54.7 bits (130), Expect = 1.4e-06
Identity = 42/133 (31.58%), Postives = 70/133 (52.63%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEEEKRKEEESLR-RKKEEKKALREESAKREEERRKKERE 61
           K +K+ E E++K   ++  K EE+KR+EEE +R +K+ E+K    E A+RE +R+K E  
Sbjct: 706 KKLKKKEKEKEKKRQQQLAKEEEKKRQEEEEIRLKKEAEEK----EIARREAQRKKVEEA 765

Query: 62  KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSP 121
           K+K            KRKKK    L E + +  E +  +K+K+E    ++  Q+ ++M  
Sbjct: 766 KRKNDE---------KRKKK----LAEQRRREEEQERIRKEKEEQKRQREEEQKQKKMEK 821

Query: 122 LKPRLRPEELGLI 134
            + +   EE  L+
Sbjct: 826 ERKQREFEEQRLL 821


HSP 3 Score: 53.9 bits (128), Expect = 2.4e-06
Identity = 36/116 (31.03%), Postives = 61/116 (52.59%), Query Frame = 1

Query: 5   KELEAEE----KKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREE--ERRKKE 64
           K  E EE    K+AE KE  + E +++K EE+ R+  E++K    E  +REE  ER +KE
Sbjct: 730 KRQEEEEIRLKKEAEEKEIARREAQRKKVEEAKRKNDEKRKKKLAEQRRREEEQERIRKE 789

Query: 65  REKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQE 115
           +E++K  R      K +++++K  E   +   K  E +  +K K+   +++K  Q+
Sbjct: 790 KEEQKRQREEEQKQKKMEKERKQREFEEQRLLKKKEAEQLQKLKENQKLNEKSMQK 845

BLAST of MELO3C025954T1 vs. Swiss-Prot
Match: NST1_EMENI (Stress response protein nst1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) GN=nst1 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.1e-10
Identity = 39/133 (29.32%), Postives = 76/133 (57.14%), Query Frame = 1

Query: 9   AEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKK---EREKKKVT 68
           AEE  A+A++++K EE+++K+EE  ++++ E+KA  EE A++E E+ K+   ERE+++  
Sbjct: 519 AEEAAAKAEQERKLEEQRKKKEEQRKKREAERKAQEEERARKEAEKHKRLREERERQEEA 578

Query: 69  RRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKPRL 128
            R     K  ++K++      E + +    Q AK++++    D++  +E ER++  + + 
Sbjct: 579 ERRQREQKEQEKKRREEAKRKEREERELREQKAKQERERKAQDEQSRRERERVAQEQAKR 638

Query: 129 RPEELGLISVVQP 139
            P  L  +S V P
Sbjct: 639 AP-NLQPVSPVSP 650


HSP 2 Score: 58.9 bits (141), Expect = 7.5e-08
Identity = 36/137 (26.28%), Postives = 75/137 (54.74%), Query Frame = 1

Query: 8   EAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREE-ERRKKEREKKKVTR 67
           E  +KK E ++K++A E K +EEE  R++ E+ K LREE  ++EE ERR++E+++++  R
Sbjct: 534 EQRKKKEEQRKKREA-ERKAQEEERARKEAEKHKRLREERERQEEAERRQREQKEQEKKR 593

Query: 68  RTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQ-KFSQEIERMSPLKPRL 127
           R     K  + ++   +   + + + A+ + ++++++    +Q K +  ++ +SP+ P  
Sbjct: 594 REEAKRKEREERELREQKAKQERERKAQDEQSRRERERVAQEQAKRAPNLQPVSPVSPPP 653

Query: 128 RPEELGLISVVQPKKGK 143
                 +     P KG+
Sbjct: 654 ESATPVVSKAPTPAKGR 669


HSP 3 Score: 55.8 bits (133), Expect = 6.4e-07
Identity = 40/136 (29.41%), Postives = 69/136 (50.74%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEE-EKRKEEESLRR--------KKEEKKALREESAKREE 61
           K ++EL  EE   E +  KKA E +KRK+++ L +        ++E +KA  E +AK E+
Sbjct: 469 KLIEELLEEENLNEQRNAKKAREAQKRKDKKRLAKLAKDEEKARREAQKAAEEAAAKAEQ 528

Query: 62  ERRKKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFS 121
           ER+ +E+ KKK  +R    A+   ++++      E   +L E +  +++ +    +QK  
Sbjct: 529 ERKLEEQRKKKEEQRKKREAERKAQEEERARKEAEKHKRLREERERQEEAERRQREQK-E 588

Query: 122 QEIERMSPLKPRLRPE 129
           QE +R    K + R E
Sbjct: 589 QEKKRREEAKRKEREE 603

BLAST of MELO3C025954T1 vs. Swiss-Prot
Match: NST1_CANAL (Stress response protein NST1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=NST1 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.8e-10
Identity = 49/126 (38.89%), Postives = 73/126 (57.94%), Query Frame = 1

Query: 5   KELEA-EEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKK 64
           +EL+A EE++   KEK KAE++KRKEE  L++++E+KK + E+  K EE R+K E ++K+
Sbjct: 762 EELKAKEEEQRLQKEKLKAEQKKRKEEARLKKEEEKKKKIEEQKRKEEEHRKKVEAQQKR 821

Query: 65  VTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKP 124
                   AK LK +++  ++  E K K  E     KK+KE L  QK  +E +R   L  
Sbjct: 822 -----EAEAKKLKEERRR-KAEEERKQKEEE-----KKQKELLKKQK--EEEKRQKELLR 874

Query: 125 RLRPEE 130
           + R EE
Sbjct: 882 KQREEE 874


HSP 2 Score: 60.8 bits (146), Expect = 2.0e-08
Identity = 51/148 (34.46%), Postives = 79/148 (53.38%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKK-----AEEEKRKEEESLRRKKEE----KKALREESAKREEERR 64
           +EL+  ++K +AKEKK+      EEE++++EE L+ K+EE    K+ L+ E  KR+EE R
Sbjct: 731 RELKKLKQKEKAKEKKRLQQLAKEEERKRKEEELKAKEEEQRLQKEKLKAEQKKRKEEAR 790

Query: 65  -KKEREKKK---------VTRRTPISAKCLKRKKKNVESLVETKTKLAEIQ----TAKKK 124
            KKE EKKK            R  + A+  ++++   + L E + + AE +      +KK
Sbjct: 791 LKKEEEKKKKIEEQKRKEEEHRKKVEAQ--QKREAEAKKLKEERRRKAEEERKQKEEEKK 850

Query: 125 KKETLMDQKFSQEIERMSPLKPRLRPEE 130
           +KE L  QK  +E +R   L  + R EE
Sbjct: 851 QKELLKKQK--EEEKRQKELLRKQREEE 874


HSP 3 Score: 60.8 bits (146), Expect = 2.0e-08
Identity = 48/139 (34.53%), Postives = 73/139 (52.52%), Query Frame = 1

Query: 2   KFVKELEAEE------------KKAEAKEKKK-----AEEEKRKEEESLRRKKEE----K 61
           K ++ELEAEE            +K +AKEKK+      EEE++++EE L+ K+EE    K
Sbjct: 716 KLIEELEAEENAKKERELKKLKQKEKAKEKKRLQQLAKEEERKRKEEELKAKEEEQRLQK 775

Query: 62  KALREESAKREEERR-KKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKL-AEIQTA 118
           + L+ E  KR+EE R KKE EKKK            KRK++     VE + K  AE +  
Sbjct: 776 EKLKAEQKKRKEEARLKKEEEKKKKIEEQ-------KRKEEEHRKKVEAQQKREAEAKKL 835


HSP 4 Score: 59.3 bits (142), Expect = 5.8e-08
Identity = 52/166 (31.33%), Postives = 79/166 (47.59%), Query Frame = 1

Query: 5   KELEAEEK--------KAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKR-EEERR 64
           ++L+AE+K        K E ++KKK EE+KRKEEE   RKK E +  RE  AK+ +EERR
Sbjct: 776 EKLKAEQKKRKEEARLKKEEEKKKKIEEQKRKEEE--HRKKVEAQQKREAEAKKLKEERR 835

Query: 65  KKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEI 124
           +K  E++K         + LK++K+  +   E   K  E +  K+K+   L +++    +
Sbjct: 836 RKAEEERKQKEEEKKQKELLKKQKEEEKRQKELLRKQREEE--KEKEAARLEEERTKLMV 895

Query: 125 ERMSPLKPRLRPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEP 162
                L  ++  E             K+K  A      LLN LY+P
Sbjct: 896 NDDDELARQIEVE-------------KSKLSAAVANNPLLNHLYQP 924

BLAST of MELO3C025954T1 vs. TrEMBL
Match: E0RWQ1_BUTPB (GDSL-family lipase/acylhydrolase OS=Butyrivibrio proteoclasticus (strain ATCC 51982 / DSM 14932 / B316) GN=bpr_I1842 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 4.6e-12
Identity = 50/131 (38.17%), Postives = 78/131 (59.54%), Query Frame = 1

Query: 4   VKELEAEEKKAEA-KEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKK 63
           +++ +AEE+K +A +EKKKAEEEK+K EE  ++ +EEKK   EE  K EEE++K E EKK
Sbjct: 385 LEKKKAEEEKKKAEEEKKKAEEEKKKAEEEKKKAEEEKKKKAEEEKKAEEEKKKAEEEKK 444

Query: 64  KVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKK----KKKETLMDQKFSQEIERM 123
           K        A+  K+K +  +   E + K AE +  KK    KKK    +QK ++E ++ 
Sbjct: 445 KAEEEEKKKAEEEKKKAEEEQKKAEEEKKKAEEEEQKKAEEEKKKAEEEEQKKAEEEKKK 504

Query: 124 SPLKPRLRPEE 130
           +  + + + EE
Sbjct: 505 AEEEEKKKVEE 515

BLAST of MELO3C025954T1 vs. TrEMBL
Match: E0RWQ1_BUTPB (GDSL-family lipase/acylhydrolase OS=Butyrivibrio proteoclasticus (strain ATCC 51982 / DSM 14932 / B316) GN=bpr_I1842 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.5e-10
Identity = 49/131 (37.40%), Postives = 71/131 (54.20%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           K+ E E+KKAE ++KKKAEEEK+ EEE  + ++E+KKA  EE  K EEE++K E E+KK 
Sbjct: 409 KKAEEEKKKAEEEKKKKAEEEKKAEEEKKKAEEEKKKAEEEEKKKAEEEKKKAEEEQKKA 468

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKK----KKKETLMDQKFSQEIERMSP 124
                      K+ ++  +   E + K AE +  KK    KKK    ++K  +E  +   
Sbjct: 469 EEEK-------KKAEEEEQKKAEEEKKKAEEEEQKKAEEEKKKAEEEEKKKVEEENKEES 528

Query: 125 LKPRLRPEELG 132
            K     EE G
Sbjct: 529 EKKEGEKEENG 532


HSP 2 Score: 37.0 bits (84), Expect = 3.4e+01
Identity = 24/59 (40.68%), Postives = 33/59 (55.93%), Query Frame = 1

Query: 5   KELEAEE--KKAEAKEKKKAEEEKRKEEESLRRKKEEK--------KALREESAKREEE 54
           ++ +AEE  KKAE +EKKK EEE ++E E    +KEE         +AL   +   EEE
Sbjct: 494 EQKKAEEEKKKAEEEEKKKVEEENKEESEKKEGEKEENGNSGNSAGEALEGSAGHSEEE 552


HSP 3 Score: 77.4 bits (189), Expect = 2.3e-11
Identity = 49/142 (34.51%), Postives = 77/142 (54.23%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           K    E ++A+A+EKKKAEE +R + E  ++ +E +KA  EE  K EE  R K  EKKK 
Sbjct: 359 KRAAEERERAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKKA 418

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAK--KKKKETLMDQKFSQEIERMSPLK 124
             R    A+  K+ ++   +  E K K  E + AK  +KKK    ++  ++E +R +  K
Sbjct: 419 EEREKAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKRAAEEK 478

Query: 125 PRLRPEELGLISVVQPKKGKNK 145
            +L  E+L     V+ KK ++K
Sbjct: 479 AKLEEEKLKAKQKVEEKKIEDK 500

BLAST of MELO3C025954T1 vs. TrEMBL
Match: M7C3L9_CHEMY (Caldesmon OS=Chelonia mydas GN=UY3_07777 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.6e-09
Identity = 53/160 (33.12%), Postives = 77/160 (48.12%), Query Frame = 1

Query: 8   EAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKK-------ALREESAKREEERRKKERE 67
           E E++ AE +E+ KAEEEKR  EE  R K EEKK       A  EE  K EE  + K  E
Sbjct: 341 EEEKRAAEERERAKAEEEKRAAEERERAKAEEKKKAEERERAKAEEKKKAEEREKAKAEE 400

Query: 68  KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAK----------------KKKKE 127
           KKK   R    A+  K+ ++  ++  E K K  E + AK                +KKK 
Sbjct: 401 KKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKKKA 460

Query: 128 TLMDQKFSQEIERMSPLKPRLRPEELGLISVVQPKKGKNK 145
              ++  ++E +R +  K +L  E+L     V+ KK ++K
Sbjct: 461 EERERAKAEEKKRAAEEKAKLEEEKLKAKQKVEEKKIEDK 500


HSP 2 Score: 68.2 bits (165), Expect = 1.4e-08
Identity = 53/171 (30.99%), Postives = 85/171 (49.71%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           K+   E +KA+A+EKKKAEE +R + E  ++ +E +KA  EE  K EE  R K  EKKK 
Sbjct: 387 KKKAEEREKAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKKA 446

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAE----IQTAKKKKKETLMDQKFSQEIERMSP 124
             R    A+  K+ ++   +  E K + AE    ++  K K K+ + ++K   +  +   
Sbjct: 447 EEREKAKAEEKKKAEERERAKAEEKKRAAEEKAKLEEEKLKAKQKVEEKKIEDKKVQDKQ 506

Query: 125 LKPRLRPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEPIK-ALKSEKI 171
           +K +   E+      VQ +  K +T +LR  G    +  E  K  L  EK+
Sbjct: 507 VKEKKIEEKQVKGKKVQEE--KPQTVSLRKQGEEKEVKVEAKKEKLPDEKL 555


HSP 3 Score: 63.5 bits (153), Expect = 3.4e-07
Identity = 47/160 (29.38%), Postives = 78/160 (48.75%), Query Frame = 1

Query: 5   KELEAEEKKA-EAKEKKKAEEEKR--------KEEESLRRKKEEKKALREESAKREEERR 64
           + ++AE+++A E +E+ KAEEEKR        K EE  R  +E ++A  EE  K EE  R
Sbjct: 322 ERIQAEQQRAAEERERAKAEEEKRAAEERERAKAEEEKRAAEERERAKAEEKKKAEERER 381

Query: 65  KKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAK-KKKKETLMDQKFSQE 124
            K  EKKK   R    A+  K+ ++   +  E K K  E + AK ++KK+    ++   E
Sbjct: 382 AKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAE 441

Query: 125 IERMSPLKPRLRPEELGLISVVQPKKGKNKTGALRHLGRL 155
            ++ +  + + + EE       +  K + K  A     +L
Sbjct: 442 EKKKAEEREKAKAEEKKKAEERERAKAEEKKRAAEEKAKL 481


HSP 4 Score: 57.0 bits (136), Expect = 3.2e-05
Identity = 45/144 (31.25%), Postives = 73/144 (50.69%), Query Frame = 1

Query: 7   LEAEEKKA-EAKEKKKAEEEKRKEEESLR--RKKEEKKALREESAKREEERRKKEREKKK 66
           +EAEEK+A + KE+ +AEE +R + E  R   ++E  KA  E+ A  E ER K E EK+ 
Sbjct: 302 IEAEEKRAAQEKERIEAEERERIQAEQQRAAEERERAKAEEEKRAAEERERAKAEEEKRA 361

Query: 67  VTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKP 126
              R    A+  K+ ++   +  E K K  E + AK ++K      K ++E ER +  + 
Sbjct: 362 AEERERAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEK------KKAEERER-AKAEE 421

Query: 127 RLRPEELGLISVVQPKKGKNKTGA 148
           + + EE       + KK + +  A
Sbjct: 422 KKKAEEREKAKAEEKKKAEERERA 438


HSP 5 Score: 56.6 bits (135), Expect = 4.2e-05
Identity = 45/140 (32.14%), Postives = 68/140 (48.57%), Query Frame = 1

Query: 8   EAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALRE----------------ESAKRE 67
           E E  +AE +E+ KAEE+K+ EEE  R + EEK+A +E                + A  E
Sbjct: 275 ERERLEAEERERVKAEEQKKVEEERARIEAEEKRAAQEKERIEAEERERIQAEQQRAAEE 334

Query: 68  EERRKKEREKKKVTRRTPISAKCLKRKKKNVE-SLVETKTKLAEIQTAK-KKKKETLMDQ 127
            ER K E EK+    R    A+  KR  +  E +  E K K  E + AK ++KK+    +
Sbjct: 335 RERAKAEEEKRAAEERERAKAEEEKRAAEERERAKAEEKKKAEERERAKAEEKKKAEERE 394

Query: 128 KFSQEIERMSPLKPRLRPEE 130
           K   E ++ +  + R + EE
Sbjct: 395 KAKAEEKKKAEERERAKAEE 414


HSP 6 Score: 52.0 bits (123), Expect = 1.0e-03
Identity = 41/146 (28.08%), Postives = 77/146 (52.74%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKE-EESLRRKKEEKKALREESAK--REEERRKKEREK 64
           ++L  EE++   +EKK+ EE +R E EE  R K EE+K + EE A+   EE+R  +E+E+
Sbjct: 256 EKLRNEEEEKTEEEKKRTEERERLEAEERERVKAEEQKKVEEERARIEAEEKRAAQEKER 315

Query: 65  KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPL 124
            +   R  I A+  +  ++   +  E + + AE +   K ++E    ++ ++E ER +  
Sbjct: 316 IEAEERERIQAEQQRAAEERERAKAEEEKRAAEERERAKAEEE----KRAAEERER-AKA 375

Query: 125 KPRLRPEELGLISVVQPKKGKNKTGA 148
           + + + EE       + KK + +  A
Sbjct: 376 EEKKKAEERERAKAEEKKKAEEREKA 396


HSP 7 Score: 40.8 bits (94), Expect = 2.4e+00
Identity = 29/82 (35.37%), Postives = 44/82 (53.66%), Query Frame = 1

Query: 10  EEKKAEAKEKKKAEEEKRK--EEESLRRKKEEKKALREESAKREEERRKKEREKKKVTRR 69
           +E   E +E KK  EE+RK  EEE  RRK+       EE+ ++  E  +K R K+ + RR
Sbjct: 664 QEAAVELEELKKKREERRKILEEEEQRRKQ-------EEAERKAREEEEKRRLKEDIERR 723

Query: 70  TPISAKCLKRKKKNVESLVETK 90
              +A+  KR+K   + L + K
Sbjct: 724 RAEAAE--KRQKMPEDGLSDDK 736


HSP 8 Score: 38.9 bits (89), Expect = 9.0e+00
Identity = 42/179 (23.46%), Postives = 82/179 (45.81%), Query Frame = 1

Query: 1   MKFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKERE 60
           MK     E  +  ++A+  K+ EE +R+  E+   + E+ K  ++E+A   EE +KK  E
Sbjct: 620 MKATTNAEDGKPVSQAEAGKRLEELRRRRGENESEEFEKLKEKQQEAAVELEELKKKREE 679

Query: 61  KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAE------IQTAKKKKK---ETLMDQKF 120
           ++K+        K  + ++K  E   E K +L E       + A+K++K   + L D K 
Sbjct: 680 RRKILEEEEQRRKQEEAERKAREE--EEKRRLKEDIERRRAEAAEKRQKMPEDGLSDDK- 739

Query: 121 SQEIERMSPLKPRLRPEELG-LISVVQPKKGKNKTGALRHLGRLLNLLYEPIKALKSEK 170
            +  +  +P    L+ EE    ++    K G   T +   + ++ + L +   A++  K
Sbjct: 740 -KPFKCFTPKGSSLKIEERAEFLNKSAQKSGAKPTHSAAIVSKIDSRLEQYTSAIEGTK 794


HSP 9 Score: 37.4 bits (85), Expect = 2.6e+01
Identity = 44/170 (25.88%), Postives = 78/170 (45.88%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKK-KAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKERE 61
           K V++ + +EKK E K+ K K  +E++ +  SLR++ EEK+   E  AK+E+   +K + 
Sbjct: 500 KKVQDKQVKEKKIEEKQVKGKKVQEEKPQTVSLRKQGEEKEVKVE--AKKEKLPDEKLQP 559

Query: 62  KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAE--IQTAKKKKKETLMDQKFSQEIERM 121
             K  +     A   K  K  V+ + + K    E   Q  ++ ++ T    K ++ I   
Sbjct: 560 TFKKQQIKDDKANKDKAPKDEVKPIWDRKKGFPEPKPQNGERFQEPTTHKLKHTENIFSR 619

Query: 122 SPLKPRLRPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEPIKALKSE 169
           S +K     E+   +S  Q + GK      R  G   +  +E +K  + E
Sbjct: 620 SGMKATTNAEDGKPVS--QAEAGKRLEELRRRRGENESEEFEKLKEKQQE 665


HSP 10 Score: 75.1 bits (183), Expect = 1.1e-10
Identity = 44/122 (36.07%), Postives = 76/122 (62.30%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           ++ EAEEK+ + +E KK EEEK+K++E L+R ++EK+ L EE+ K EEER++KE E+KK 
Sbjct: 452 RQKEAEEKRKKEEELKKMEEEKKKKQEELKRIEQEKQRLAEEAKKAEEERKQKELEEKK- 511

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAEI---------QTAKKKKKETLMDQKFSQEI 118
            RR     K  + +++  +   E + K  E+         + AK++K++    ++ ++EI
Sbjct: 512 -RRDEELRKQREEERRRQQEEDERRRKEEELLAKQRALEEEDAKRRKQQEEEQKRLAEEI 571

BLAST of MELO3C025954T1 vs. TrEMBL
Match: A2FHD4_TRIVA (Trichohyalin, putative OS=Trichomonas vaginalis GN=TVAG_144410 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-07
Identity = 37/110 (33.64%), Postives = 65/110 (59.09%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
            KELE ++KK++ + +KK EEE RK EE+ R++KE+++   EE  +R EE +++  E KK 
Sbjct: 1033 KELEEQQKKSDEERRKKREEEDRKAEEARRKRKEQEEKEAEERRQRYEEEQRQFEEDKK- 1092

Query: 65   TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQE 115
             RR     K  + ++K+ E L     K ++ +   +K     + +KF++E
Sbjct: 1093 -RREEEEQKQQEERRKHFEELAAQLEKRSKQKLEDEKNALENLRKKFAEE 1140


HSP 2 Score: 62.4 bits (150), Expect = 7.6e-07
Identity = 52/172 (30.23%), Postives = 87/172 (50.58%), Query Frame = 1

Query: 6    ELEAEEKKAEAKE-----KKKAEEE---KRKEEESLRRKK---EEKKALREESAKREEER 65
            E+EAE+K+ EA+E     KK+AEEE   KRKE E   RKK    E++A R++ A +EE R
Sbjct: 1396 EIEAEKKRKEAEEEAERKKKEAEEEAEKKRKEAEEEARKKMEEAEEEARRKKEAAKEERR 1455

Query: 66   RKK---EREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKF 125
            RKK   E E ++  +    + K  +RKK+  + L       AE++  + +K+     ++ 
Sbjct: 1456 RKKAEAEAEAERKRKEVEEAEKEAQRKKEEADKL------QAELEKLRAQKEAEAEAERQ 1515

Query: 126  SQEIERMSPLKPRLRPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEPIK 164
             + + +    + R+R EE  L    + ++ + +    R +  L     EP K
Sbjct: 1516 RERLRKKQEEEERMREEERRLAEEAEKRRQEEEERRRREIEILTLEEAEPTK 1561


HSP 3 Score: 62.4 bits (150), Expect = 7.6e-07
Identity = 40/121 (33.06%), Postives = 70/121 (57.85%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEE--KRKEEESLRRKKEEKKALREESAKREEERRKKEREKK 64
           K+ E E++K EA+EK++ EEE  ++KE E  R+K+EE K + EE  K++EE ++ E+EK+
Sbjct: 429 KKEEEEKQKKEAEEKRRKEEEEKRQKEAEEKRKKEEELKKMEEEKKKKQEELKRIEQEKQ 488

Query: 65  KVTRRTPIS-----AKCLKRKKKNVESL----VETKTKLAEIQTAKKKKKETLMDQKFSQ 115
           ++      +      K L+ KK+  E L     E + +  E    ++K++E L  Q+  +
Sbjct: 489 RLAEEAKKAEEERKQKELEEKKRRDEELRKQREEERRRQQEEDERRRKEEELLAKQRALE 548


HSP 4 Score: 60.8 bits (146), Expect = 2.2e-06
Identity = 43/136 (31.62%), Postives = 70/136 (51.47%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEE------KRKEEESLRRKKEEKKALRE-ESAKREEERRKK 64
            K+L+ EE KAE   KK+ EEE       RK+ E+L +  EE + LRE E    EE R+K+
Sbjct: 875  KKLQEEEMKAEQARKKRQEEEDKMIEDSRKKREALEKLVEEARKLREGEERMAEEARKKR 934

Query: 65   EREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQ---- 124
            E E K +  R     + L+R  +      E + + AE++  K++++E    +K  Q    
Sbjct: 935  EEEDKAMEERKQQKLEELERIAEEARKKREEEARQAELEMKKRREEEEKEHEKERQKKID 994

Query: 125  EIERMSPLKPRLRPEE 130
            E  ++   + ++R EE
Sbjct: 995  EENKLLEQRRKMREEE 1010


HSP 5 Score: 59.7 bits (143), Expect = 4.9e-06
Identity = 40/168 (23.81%), Postives = 86/168 (51.19%), Query Frame = 1

Query: 1   MKFVKELEAEEKKAEAKEKKKAEEE----KRKEEESL------------------RRKKE 60
           M+ + E E + +K + K KK+ EEE    KRK++E++                  +  +E
Sbjct: 813 MRKIAEEEEKRRKEDEKRKKELEEEEKERKRKQKEAMEKLDEAERELERLRDQHQKEDQE 872

Query: 61  EKKALREESAKREEERRKKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTA 120
            KK L+EE  K E+ R+K++ E+ K+   +    + L++  +    L E + ++AE    
Sbjct: 873 RKKKLQEEEMKAEQARKKRQEEEDKMIEDSRKKREALEKLVEEARKLREGEERMAEEARK 932

Query: 121 KKKKKETLMDQKFSQEIERMSPL--KPRLRPEELGLISVVQPKKGKNK 145
           K+++++  M+++  Q++E +  +  + R + EE    + ++ KK + +
Sbjct: 933 KREEEDKAMEERKQQKLEELERIAEEARKKREEEARQAELEMKKRREE 980


HSP 6 Score: 58.5 bits (140), Expect = 1.1e-05
Identity = 40/131 (30.53%), Postives = 73/131 (55.73%), Query Frame = 1

Query: 11  EKKAEAKEKKKAEEEKRKEEESLRRKK---------EEKKALREESAKR---EEERRKKE 70
           +K+A  +EK++ EEE+RK  E ++R+K         EE+K  +EE A+R   EEE+RKKE
Sbjct: 261 KKRAMEEEKRRKEEEERKMLEEIKRQKKAEEEKCRQEEEKRRKEEEARRQKEEEEKRKKE 320

Query: 71  REKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERM 130
            E++K        A+  +++++  +   E K +    Q  ++K+++    +K  +EI+R 
Sbjct: 321 EEERKRIEEEKRQAEERQKRREERKRREEEKRR----QEEEEKRRQEEEKRKQEEEIKRK 380


HSP 7 Score: 58.2 bits (139), Expect = 1.4e-05
Identity = 39/128 (30.47%), Postives = 72/128 (56.25%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
            K+LE EEK+AE + +++ +EE    E  +RR+K EK+A  E   K  EE     ++ K+ 
Sbjct: 1264 KKLEQEEKEAEERRRQREQEEL---EAEIRREKGEKEA-EERRKKMIEEAENLLKQAKEE 1323

Query: 65   TRRTPISAKCLKRKKKNVESLVETKTK---LAEIQTAKKKKKETLMDQKFSQEIERMSPL 124
              +    A+  +++K+ +++ +E K K    AE +T +K+K+     +K  +E E+++ L
Sbjct: 1324 AEKKNREAEEARKRKEEMDAELERKKKEAEEAEKETQRKRKEAEEEAKKLKEEAEKLAEL 1383

Query: 125  KPRLRPEE 130
            K +   EE
Sbjct: 1384 KQKQAEEE 1387


HSP 8 Score: 57.8 bits (138), Expect = 1.9e-05
Identity = 45/129 (34.88%), Postives = 68/129 (52.71%), Query Frame = 1

Query: 1    MKFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKERE 60
            +K  KE EAE+K  EA+E +K +EE   E E  R+KKE ++A +E   KR+E     E E
Sbjct: 1313 LKQAKE-EAEKKNREAEEARKRKEEMDAELE--RKKKEAEEAEKETQRKRKE----AEEE 1372

Query: 61   KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSP 120
             KK+      + K  + K+K  E   E K + AEI+  KK+K+     ++  +E E  + 
Sbjct: 1373 AKKLKEE---AEKLAELKQKQAEEEAEKKRREAEIEAEKKRKEAEEEAERKKKEAEEEAE 1431

Query: 121  LKPRLRPEE 130
             K +   EE
Sbjct: 1433 KKRKEAEEE 1431


HSP 9 Score: 57.8 bits (138), Expect = 1.9e-05
Identity = 39/126 (30.95%), Postives = 65/126 (51.59%), Query Frame = 1

Query: 8    EAEEKKAEAKEKKKAEEEKRKEE------ESLRRKKEEKKALREESA---------KREE 67
            E EE+ AE   KK+ EE+K  EE      E L R  EE +  REE A         +REE
Sbjct: 921  EGEERMAEEARKKREEEDKAMEERKQQKLEELERIAEEARKKREEEARQAELEMKKRREE 980

Query: 68   ERRKKEREK-KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKF 118
            E ++ E+E+ KK+     +  +  K +++  ++  E K K+A+     ++K++ L +Q+ 
Sbjct: 981  EEKEHEKERQKKIDEENKLLEQRRKMREEEEKAAEELKRKIAQDMALSEQKRKELEEQQK 1040


HSP 10 Score: 53.9 bits (128), Expect = 2.7e-04
Identity = 39/129 (30.23%), Postives = 70/129 (54.26%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKK---EEKKALREESAKREEERRKKEREK 64
           K+L  EE +   K ++++ E ++K +E L +KK   EE + LR++ A  EE  RKK+ E 
Sbjct: 726 KKLSDEEAEIRRKMEEQSAEARKKLQEELDQKKKQHEEDERLRKQKADEEETERKKKLED 785

Query: 65  KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPL 124
           +    R  +  +  +RK+K  +   E   K+AE +  +K++KE   D+K  +E+E     
Sbjct: 786 ELEKHRKRLDEEEKQRKEKAKKEDEERMRKIAEEE--EKRRKE---DEKRKKELEEEEKE 845

Query: 125 KPRLRPEEL 131
           + R + E +
Sbjct: 846 RKRKQKEAM 849


HSP 11 Score: 53.5 bits (127), Expect = 3.5e-04
Identity = 42/138 (30.43%), Postives = 70/138 (50.72%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKK--EEKKALREESAKREEERRKKEREKK 64
            K+ E E ++AE + KK+ EEE+ KE E  R+KK  EE K L +    REEE +  E  K+
Sbjct: 961  KKREEEARQAELEMKKRREEEE-KEHEKERQKKIDEENKLLEQRRKMREEEEKAAEELKR 1020

Query: 65   KVTRRTPISAKCLK-----RKKKNVESLVETKTKLAEIQTAKKKKKE------TLMDQKF 124
            K+ +   +S +  K     +KK + E   + + +  + + A++K+KE          Q++
Sbjct: 1021 KIAQDMALSEQKRKELEEQQKKSDEERRKKREEEDRKAEEARRKRKEQEEKEAEERRQRY 1080

Query: 125  SQEIERMSPLKPRLRPEE 130
             +E  +    K R   EE
Sbjct: 1081 EEEQRQFEEDKKRREEEE 1097


HSP 12 Score: 52.8 bits (125), Expect = 6.0e-04
Identity = 42/143 (29.37%), Postives = 76/143 (53.15%), Query Frame = 1

Query: 8    EAEEKKAEAKEKKKAEEEKR------KEEESLRRKKE----EKKALREESAKREEERRKK 67
            +AE +   A+E+K+AEE ++      KE E  RR++E    E +  RE+  K  EERRKK
Sbjct: 1245 DAERRARIAQEEKEAEERRKKLEQEEKEAEERRRQREQEELEAEIRREKGEKEAEERRKK 1304

Query: 68   E-REKKKVTRRTPISAKC-------LKRKKKNVESLVETKTK---LAEIQTAKKKKKETL 127
               E + + ++    A+         +++K+ +++ +E K K    AE +T +K+K+   
Sbjct: 1305 MIEEAENLLKQAKEEAEKKNREAEEARKRKEEMDAELERKKKEAEEAEKETQRKRKEAEE 1364

Query: 128  MDQKFSQEIERMSPLKPRLRPEE 130
              +K  +E E+++ LK +   EE
Sbjct: 1365 EAKKLKEEAEKLAELKQKQAEEE 1387


HSP 13 Score: 51.2 bits (121), Expect = 1.8e-03
Identity = 33/133 (24.81%), Postives = 68/133 (51.13%), Query Frame = 1

Query: 9    AEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKA---------LREESAKREEERRKKER 68
            AEE+ AE + +KK E E ++E+E  R+++ ++ A         ++E++ + E  RR++E+
Sbjct: 1138 AEEEAAEEERRKKREREDKEEDEERRKRRAKEDAEWEARRQRRMQEDAEEEEARRRRREQ 1197

Query: 69   EKKKVT---RRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIE 128
            E+K+     RR  +  K  + K+K  E       +    +  K++K++     + +QE +
Sbjct: 1198 EEKEDAERRRRRELEEKEAEEKRKKREQEKAEDKERRRRKKEKEEKEDAERRARIAQEEK 1257

Query: 129  RMSPLKPRLRPEE 130
                 + +L  EE
Sbjct: 1258 EAEERRKKLEQEE 1270


HSP 14 Score: 50.4 bits (119), Expect = 3.0e-03
Identity = 49/205 (23.90%), Postives = 97/205 (47.32%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREK 61
           K  KE +AE  K  A E+++  ++   EE  +RRK EE+ A   +  + E +++KK+ E+
Sbjct: 705 KLEKE-KAERMKQLADEEEERRKKLSDEEAEIRRKMEEQSAEARKKLQEELDQKKKQHEE 764

Query: 62  KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKK---KKKETLMDQKFSQEIERM 121
            +  R+     +  +RKKK  + L + + +L E +  +K   KK++    +K ++E E+ 
Sbjct: 765 DERLRKQKADEEETERKKKLEDELEKHRKRLDEEEKQRKEKAKKEDEERMRKIAEEEEKR 824

Query: 122 SPLKPRLRPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEPIKALKSEKIFMGETKVR 181
              +   R +EL      + +K K     L    R L  L +  +    E+    + K++
Sbjct: 825 RK-EDEKRKKELEEEEKERKRKQKEAMEKLDEAERELERLRDQHQKEDQER----KKKLQ 884

Query: 182 LDIVNMFYARKYHPDDSYAIVEGEK 204
            + +    ARK   ++   ++E  +
Sbjct: 885 EEEMKAEQARKKRQEEEDKMIEDSR 903


HSP 15 Score: 50.4 bits (119), Expect = 3.0e-03
Identity = 42/149 (28.19%), Postives = 69/149 (46.31%), Query Frame = 1

Query: 6    ELEAEEKKAEA----------KEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERR 65
            E EA+ KK EA          + +K+AE E  ++ E LR+K+EE++ +REE  +  EE  
Sbjct: 1476 EKEAQRKKEEADKLQAELEKLRAQKEAEAEAERQRERLRKKQEEEERMREEERRLAEEAE 1535

Query: 66   KKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEI 125
            K+ +E+++  RR  I    L+  +       E    +  I+     K     D+   +++
Sbjct: 1536 KRRQEEEE-RRRREIEILTLEEAEPTKVDDQEYDEDVQIIEYVSDYKYVYDEDENEQEQV 1595

Query: 126  ERMSPLKPRLRPEELGLISVVQPKKGKNK 145
            E   P K     EE    S  +PKK  +K
Sbjct: 1596 EEEKPKK----QEEKTTKSEEKPKKHDDK 1619


HSP 16 Score: 48.9 bits (115), Expect = 8.7e-03
Identity = 33/128 (25.78%), Postives = 66/128 (51.56%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
            K  E EEK AE  ++K A++    E++    ++++KK+  E   KREEE RK E  ++K 
Sbjct: 1005 KMREEEEKAAEELKRKIAQDMALSEQKRKELEEQQKKSDEERRKKREEEDRKAEEARRKR 1064

Query: 65   TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKPR 124
              +    A+  +++ +  +   E   K  E +  K++++     ++ + ++E+ S  K +
Sbjct: 1065 KEQEEKEAEERRQRYEEEQRQFEEDKKRREEEEQKQQEERRKHFEELAAQLEKRS--KQK 1124

Query: 125  LRPEELGL 133
            L  E+  L
Sbjct: 1125 LEDEKNAL 1130


HSP 17 Score: 46.2 bits (108), Expect = 5.6e-02
Identity = 38/139 (27.34%), Postives = 70/139 (50.36%), Query Frame = 1

Query: 10   EEKKAEAKEKKKAEEEKRK---------EEESLRRKKEEKKALR-------EESAKREEE 69
            E+KK   +E++K +EE+RK         E+ S ++ ++EK AL        EE A  EE 
Sbjct: 1088 EDKKRREEEEQKQQEERRKHFEELAAQLEKRSKQKLEDEKNALENLRKKFAEEEAAEEER 1147

Query: 70   RRKKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKL---AEIQTAKKKKKETLMDQK 129
            R+K+ERE K+         +  +R K++ E     + ++   AE + A+++++E   ++K
Sbjct: 1148 RKKREREDKEEDEE-----RRKRRAKEDAEWEARRQRRMQEDAEEEEARRRRRE--QEEK 1207


HSP 18 Score: 75.1 bits (183), Expect = 1.1e-10
Identity = 45/130 (34.62%), Postives = 77/130 (59.23%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKK---EEKKALREESAKREEERRKK--ER 64
            K  E EE+K + +EK+KAEEE++++EE LR+KK   E+K+ L EE  K+EEE RKK  E 
Sbjct: 1109 KRKEEEERKRKEEEKRKAEEERKRKEEELRKKKEAEEKKRKLEEEHKKKEEELRKKKEEE 1168

Query: 65   EKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMS 124
            EK++       + +  KRK++  ++  E + ++   +  +KK++E    +K  +E+    
Sbjct: 1169 EKRRQEEEKRKAEEERKRKEEEEKARKEEEERIKREEEERKKQEEEERKKKEEEELRVKQ 1228

Query: 125  PLKPRLRPEE 130
              + + R EE
Sbjct: 1229 EEEKKKRAEE 1238

BLAST of MELO3C025954T1 vs. TrEMBL
Match: M3UWV9_ENTHI (IgA-specific serine endopeptidase, putative (Fragment) OS=Entamoeba histolytica HM-1:IMSS-B GN=EHI8A_129860 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.5e-10
Identity = 45/129 (34.88%), Postives = 75/129 (58.14%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEE-KRKEEESLRRKKEEKKALREESAKREEERRKK---ERE 64
            ++ E E++KAEA+ K+K EEE KRKEEE  +RK+EEK+   EE  ++EEE RKK   E +
Sbjct: 1087 RKQEEEKRKAEAERKRKEEEERKRKEEEERKRKEEEKRKAEEERKRKEEELRKKKEAEEK 1146

Query: 65   KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSP 124
            K+K+        + L++KK+  E   + + K    +  K+K++E    ++  + I+R   
Sbjct: 1147 KRKLEEEHKKKEEELRKKKEEEEKRRQEEEKRKAEEERKRKEEEEKARKEEEERIKREEE 1206

Query: 125  LKPRLRPEE 130
             + +   EE
Sbjct: 1207 ERKKQEEEE 1215


HSP 2 Score: 72.8 bits (177), Expect = 5.6e-10
Identity = 49/140 (35.00%), Postives = 79/140 (56.43%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
            K +EAE K+ E +E+K+ EEE+RK +E  R++KEE+    E   K EEE+RKKE E+ K 
Sbjct: 1017 KRVEAERKRKEEEERKRKEEEERKRKEEERKRKEEE----ERKRKEEEEKRKKELEELKK 1076

Query: 65   TRRTPISAK--CLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLK 124
             +      K   LKRK++  +   E + K  E +  K+K++E   ++K  +E +R +  +
Sbjct: 1077 LKEEERRKKEEELKRKQEEEKRKAEAERKRKEEEERKRKEEE---ERKRKEEEKRKAEEE 1136

Query: 125  PRLRPEELGLISVVQPKKGK 143
             + + EEL      + KK K
Sbjct: 1137 RKRKEEELRKKKEAEEKKRK 1149


HSP 3 Score: 72.0 bits (175), Expect = 9.6e-10
Identity = 53/178 (29.78%), Postives = 91/178 (51.12%), Query Frame = 1

Query: 8    EAEEKKAEAKEKKKAEEEKRKEEESLRRKK----EEKKALREESAKREEERRKKEREKKK 67
            E EEK+   +E KK EEE+RK +E++  KK    EE+K   EE  KREEE RKKE E+++
Sbjct: 843  EEEEKRKVEEELKKKEEEERKRKEAIELKKKQLEEERKKKEEERKKREEEERKKEEEEER 902

Query: 68   VTRRTPISAKCLKRKKKNVESLVETKTKLAE-IQTAKKKKKETLMDQKFSQEIERMSPLK 127
            + +      + L+ ++K  E  ++ K +  E  +  ++++K    ++K  +E ER    +
Sbjct: 903  LKQIEQEKQRKLEEERKKKEEAIKRKKEEEERKRKEEERRKREEAERKRKEEEERKRKEE 962

Query: 128  PRLRPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEPIKALKSEKIFMGETKVRLD 181
               R  E      ++ ++ K +    R L     LL E  K L+ E+    E + R++
Sbjct: 963  EAKRKIEQERQRKIEEERRKKEEEEQRRLEEEKKLLEEEQKRLEEEERKAEEERKRVE 1020


HSP 4 Score: 69.7 bits (169), Expect = 4.8e-09
Identity = 38/121 (31.40%), Postives = 69/121 (57.02%), Query Frame = 1

Query: 4    VKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEE--KKALREESAKREEERRKKEREK 63
            +KE E E K+ EA+ K+K EE+K++EEE  R+++EE  K+   EE A++EEE ++K  ++
Sbjct: 1277 LKEAEEERKRIEAERKRKEEEKKKREEEEKRKREEEERKRKEEEEKARKEEEEKRKREDE 1336

Query: 64   KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPL 123
            +++ R      K  + +K  +    E K +   ++    + KE   +++  QE ER+   
Sbjct: 1337 ERMRRHEEERRKWEEEQKARMAEFEEMKREAERLRQEAARLKEE--EERLKQEAERLKKE 1395


HSP 5 Score: 67.0 bits (162), Expect = 3.1e-08
Identity = 44/126 (34.92%), Postives = 73/126 (57.94%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKK---EEKKALREESAKR--EEERRKKER 64
           K+ E E  + E +E K+ +EE++++EE LR+KK   E K+ L EE+ KR  EEE+RK+E 
Sbjct: 786 KKKEEERLRQEEEENKRIKEERQRKEEELRKKKAEEERKRKLEEEARKRKEEEEQRKEEE 845

Query: 65  EKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKK---KKKETLMDQKFSQEIE 123
           EK+KV        K  + +++  +  +E K K  E +  KK   +KK    ++K  +E E
Sbjct: 846 EKRKVEE----ELKKKEEEERKRKEAIELKKKQLEEERKKKEEERKKREEEERKKEEEEE 905


HSP 6 Score: 61.2 bits (147), Expect = 1.7e-06
Identity = 45/127 (35.43%), Postives = 75/127 (59.06%), Query Frame = 1

Query: 4    VKELEAEEKKAEAKEKKK-AEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKK 63
            VK+ E ++K+AE +EK++ AEE KRKEEE+  RKKEE++  R +    EEER+ KE E++
Sbjct: 1226 VKQEEEKKKRAEEEEKRRRAEERKRKEEEA--RKKEEEEVERLKKELEEEERKLKEAEEE 1285

Query: 64   KVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLK 123
                R  I A+  KRK++  +   E + +  E +  K+K++E    ++  ++ +R    +
Sbjct: 1286 ----RKRIEAE-RKRKEEEKKKREEEEKRKREEEERKRKEEEEKARKEEEEKRKREDEER 1345

Query: 124  PRLRPEE 130
             R   EE
Sbjct: 1346 MRRHEEE 1345


HSP 7 Score: 35.4 bits (80), Expect = 9.9e+01
Identity = 21/60 (35.00%), Postives = 39/60 (65.00%), Query Frame = 1

Query: 10   EEKKAEAKEKKKAE----EEKRKEEESLR----RKKEEKKALREESAKREEERRKKEREK 62
            EE++ + +E++KA     EE ++E E LR    R KEE++ L++E+ + ++E+ + ER K
Sbjct: 1343 EEERRKWEEEQKARMAEFEEMKREAERLRQEAARLKEEEERLKQEAERLKKEKEETERFK 1402


HSP 8 Score: 75.1 bits (183), Expect = 1.1e-10
Identity = 45/130 (34.62%), Postives = 77/130 (59.23%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKK---EEKKALREESAKREEERRKK--ER 64
            K  E EE+K + +EK+KAEEE++++EE LR+KK   E+K+ L EE  K+EEE RKK  E 
Sbjct: 1109 KRKEEEERKRKEEEKRKAEEERKRKEEELRKKKEAEEKKRKLEEEHKKKEEELRKKKEEE 1168

Query: 65   EKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMS 124
            EK++       + +  KRK++  ++  E + ++   +  +KK++E    +K  +E+    
Sbjct: 1169 EKRRQEEEKRKAEEERKRKEEEEKARKEEEERIKREEEERKKQEEEERKKKEEEELRVKQ 1228

Query: 125  PLKPRLRPEE 130
              + + R EE
Sbjct: 1229 EEEKKKRAEE 1238

BLAST of MELO3C025954T1 vs. TAIR10
Match: AT2G18540.1 (AT2G18540.1 RmlC-like cupins superfamily protein)

HSP 1 Score: 64.7 bits (156), Expect = 7.7e-11
Identity = 41/129 (31.78%), Postives = 70/129 (54.26%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEE----KKALREESAKREEERRKKERE 64
           +E E E ++ E +E +K EE KR+EEE  +R++EE    KK   EE+ KREEER+++E E
Sbjct: 439 EEEEIERRRKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEE 498

Query: 65  KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSP 124
            K+            KR+++  ++    + +  E + AKK+++E    ++   E +R   
Sbjct: 499 AKRREEERK------KREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKRREE 558

Query: 125 LKPRLRPEE 130
            + + R EE
Sbjct: 559 QERKRREEE 561


HSP 2 Score: 62.4 bits (150), Expect = 3.8e-10
Identity = 41/126 (32.54%), Postives = 74/126 (58.73%), Query Frame = 1

Query: 8   EAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAK--REEERRKKERE--KKK 67
           E E K+ E + +K+ EE KR+EE + RR++E ++  REE  +  REE+ RK+E E  K++
Sbjct: 552 EQERKRREEEARKREEERKREEEMAKRREQERQRKEREEVERKIREEQERKREEEMAKRR 611

Query: 68  VTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKP 127
              R     + ++RKK+  E+  + + ++A+I+  ++++KE    ++  +E E M   + 
Sbjct: 612 EQERQKKEREEMERKKREEEAR-KREEEMAKIREEERQRKEREDVERKRREEEAMRREEE 671

Query: 128 RLRPEE 130
           R R EE
Sbjct: 672 RKREEE 676


HSP 3 Score: 62.0 bits (149), Expect = 5.0e-10
Identity = 40/126 (31.75%), Postives = 67/126 (53.17%), Query Frame = 1

Query: 10  EEKKAEAKEKKKAEEE----KRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKVT 69
           E K+ E +E K+ EEE    K++EEE  R+++EE+K   EE+ +REEER+K+E E ++  
Sbjct: 458 EAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRREEERKKREEEAEQAR 517

Query: 70  RRTPISAKCLKRKKKNVE-----SLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSP 127
           +R     K  +  KK  E        E + K  E Q  K++++E    ++  +  E M+ 
Sbjct: 518 KREEEREKEEEMAKKREEERQRKEREEVERKRREEQERKRREEEARKREEERKREEEMAK 577


HSP 4 Score: 61.6 bits (148), Expect = 6.6e-10
Identity = 39/126 (30.95%), Postives = 67/126 (53.17%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKA-LREESAKREEERRKKEREKKK 64
           KE E  +K+ E +++K+ EE +RK  E   RK+ E++A  REE  KREEE  K+  ++++
Sbjct: 525 KEEEMAKKREEERQRKEREEVERKRREEQERKRREEEARKREEERKREEEMAKRREQERQ 584

Query: 65  VTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKP 124
              R  +  K  + +++  E   E   K  E Q  +KK++E +  +K  +E  +      
Sbjct: 585 RKEREEVERKIREEQERKRE---EEMAKRRE-QERQKKEREEMERKKREEEARKREEEMA 644

Query: 125 RLRPEE 130
           ++R EE
Sbjct: 645 KIREEE 646


HSP 5 Score: 60.5 bits (145), Expect = 1.5e-09
Identity = 44/132 (33.33%), Postives = 75/132 (56.82%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKK--KAEEEKRKEEESLRRKKEEKKALRE--ESAKREEERRKKERE 64
           +E E + K+ E  E+K  + +E KR+EE + RR++E +K  RE  E  KREEE RK+E E
Sbjct: 579 REQERQRKEREEVERKIREEQERKREEEMAKRREQERQKKEREEMERKKREEEARKREEE 638

Query: 65  KKKV--TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERM 124
             K+    R     + ++RK++  E++   + +  E + AK+ ++E    +K  +E +R 
Sbjct: 639 MAKIREEERQRKEREDVERKRREEEAMRREEERKREEEAAKRAEEE--RRKKEEEEEKRR 698

Query: 125 SPLKPRLRPEEL 131
            P +P+  PEE+
Sbjct: 699 WPPQPK-PPEEI 707


HSP 6 Score: 58.2 bits (139), Expect = 7.3e-09
Identity = 44/130 (33.85%), Postives = 70/130 (53.85%), Query Frame = 1

Query: 10  EEKKAEAKEKKKAEEEKRKEEESLR--------RKKEEKKALREESAKREEERRKKEREK 69
           EE+K E +E K+ EEE++K EE           R+KEE     E + KREEER++KERE+
Sbjct: 490 EERKREEEEAKRREEERKKREEEAEQARKREEEREKEE-----EMAKKREEERQRKEREE 549

Query: 70  KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIER-MSP 129
            +  RR        +RK++  E+    + +  E + AK++++E    +K  +E+ER +  
Sbjct: 550 VERKRREE-----QERKRREEEARKREEERKREEEMAKRREQE--RQRKEREEVERKIRE 607

Query: 130 LKPRLRPEEL 131
            + R R EE+
Sbjct: 610 EQERKREEEM 607

BLAST of MELO3C025954T1 vs. TAIR10
Match: AT5G60030.1 (AT5G60030.1 unknown protein)

HSP 1 Score: 62.0 bits (149), Expect = 5.0e-10
Identity = 48/157 (30.57%), Postives = 81/157 (51.59%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEEEKR--KEEESLRRKKEEKKALREESAKREEERRKKER 61
           K  ++LEAE++  E +E+KK +++K+  K+E+ +  K +EK    ++SA R+E ++KK +
Sbjct: 132 KVNEKLEAEQRSEERRERKKEKKKKKNNKDEDVVDEKVKEKLEDEQKSADRKERKKKKSK 191

Query: 62  --------EKKKVTRRTPISAKCLKRKKKNVESLVE--TKTKLAEIQTAKKKKKETLMDQ 121
                   ++K+       SA+  ++KK   E +V+   K KL + Q + ++KKE    +
Sbjct: 192 KNNDEDVVDEKEKLEDEQKSAEIKEKKKNKDEDVVDEKEKEKLEDEQRSGERKKEKKKKR 251

Query: 122 KFSQEI--ERMSPLKPRLRPEELGLISVVQPKKGKNK 145
           K  +EI  E     K R   EE+G       KK K K
Sbjct: 252 KSDEEIVSEERKSKKKRKSDEEMGSEERKSKKKRKLK 288


HSP 2 Score: 41.2 bits (95), Expect = 9.2e-04
Identity = 27/103 (26.21%), Postives = 53/103 (51.46%), Query Frame = 1

Query: 17  KEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKVTRRTPISAKCLK 76
           +E+ + + +K K+ + +  K  EK    + S +R E  RKKE++KKK  +   +  + +K
Sbjct: 113 RERDEKKMKKSKDADVVDEKVNEKLEAEQRSEERRE--RKKEKKKKKNNKDEDVVDEKVK 172

Query: 77  RKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMS 120
            K ++ +   + K +  + + +KK   E ++D+K   E E+ S
Sbjct: 173 EKLEDEQKSADRKER--KKKKSKKNNDEDVVDEKEKLEDEQKS 211

BLAST of MELO3C025954T1 vs. TAIR10
Match: AT1G29000.1 (AT1G29000.1 Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 8.6e-10
Identity = 31/74 (41.89%), Postives = 47/74 (63.51%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREK 61
           K  K  E    K E ++KK+ E++K+KEEE  ++K++EKK  +EE  K+EEE +KKE EK
Sbjct: 170 KVHKHAEIISSKTEEEKKKEEEDKKKKEEEDKKKKEDEKK--KEEEKKKEEENKKKEGEK 229

Query: 62  KKVTRRTPISAKCL 76
           KK   +  ++ K +
Sbjct: 230 KKEEVKVEVTTKTI 241


HSP 2 Score: 35.8 bits (81), Expect = 3.9e-02
Identity = 25/81 (30.86%), Postives = 38/81 (46.91%), Query Frame = 1

Query: 36  KKEEKKALREESAKREEERRKKEREKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEI 95
           KK+  K     S+K EEE++K+E +KKK  +      K    KKK  E   E + K  E 
Sbjct: 168 KKKVHKHAEIISSKTEEEKKKEEEDKKK--KEEEDKKKKEDEKKKEEEKKKEEENKKKEG 227

Query: 96  QTAKKKKKETLMDQKFSQEIE 117
           +  K++ K  +  +  +Q +E
Sbjct: 228 EKKKEEVKVEVTTKTITQVVE 246

BLAST of MELO3C025954T1 vs. TAIR10
Match: AT3G28770.1 (AT3G28770.1 Protein of unknown function (DUF1216))

HSP 1 Score: 60.5 bits (145), Expect = 1.5e-09
Identity = 42/144 (29.17%), Postives = 80/144 (55.56%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEER------RKKE 64
            ++L+A++K+ E KEKK++E  K K++E  +++ E+ K++++E  K+E+++      RKKE
Sbjct: 1056 RDLKAKKKEEETKEKKESENHKSKKKED-KKEHEDNKSMKKEEDKKEKKKHEESKSRKKE 1115

Query: 65   REKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERM 124
             +KK + +    ++   K+K+   E       KL + ++ KK+KKE   ++  ++EIE  
Sbjct: 1116 EDKKDMEKLEDQNSN--KKKEDKNEKKKSQHVKLVKKESDKKEKKEN-EEKSETKEIESS 1175

Query: 125  SPLKPRLRPEELGLISVVQPKKGK 143
               K  +  +E       Q KK K
Sbjct: 1176 KSQKNEVDKKEKKSSKDQQKKKEK 1195


HSP 2 Score: 58.5 bits (140), Expect = 5.6e-09
Identity = 46/152 (30.26%), Postives = 82/152 (53.95%), Query Frame = 1

Query: 2    KFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRK-KEEKKALREESAKREEERRKKERE 61
            K  +E +++ K+   KEKKK++++KR+E++S  RK K+EK+  R+  AK++EE  K+++E
Sbjct: 1013 KEYEEKKSKTKEEAKKEKKKSQDKKREEKDSEERKSKKEKEESRDLKAKKKEEETKEKKE 1072

Query: 62   ------KKKVTRRTPISAKCLKRK--KKNVESLVETKTKLAEIQTAKKKKKETLMDQKFS 121
                  KKK  ++     K +K++  KK  +   E+K++  E     KK  E L DQ  +
Sbjct: 1073 SENHKSKKKEDKKEHEDNKSMKKEEDKKEKKKHEESKSRKKE---EDKKDMEKLEDQNSN 1132

Query: 122  QEIERMSPLKPRLRPEELGLISVVQPKKGKNK 145
            ++ E  +  K   + + + L+     KK K +
Sbjct: 1133 KKKEDKNEKK---KSQHVKLVKKESDKKEKKE 1158


HSP 3 Score: 55.8 bits (133), Expect = 3.6e-08
Identity = 38/129 (29.46%), Postives = 69/129 (53.49%), Query Frame = 1

Query: 8    EAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKVTRR 67
            E E+ K+  KE+ K E++K +E +S R+K+E+KK + +   +   ++++ + EKKK    
Sbjct: 1086 EHEDNKSMKKEEDKKEKKKHEESKS-RKKEEDKKDMEKLEDQNSNKKKEDKNEKKKSQHV 1145

Query: 68   TPISAKCLKRKKKNVESLVETKTKLAEIQTAK-------KKKKETLMDQKFSQEIERMSP 127
              +  +  K++KK  E   ETK    EI+++K       KK+K++  DQ+  +E E    
Sbjct: 1146 KLVKKESDKKEKKENEEKSETK----EIESSKSQKNEVDKKEKKSSKDQQKKKEKEMKES 1205

Query: 128  LKPRLRPEE 130
             + +L+  E
Sbjct: 1206 EEKKLKKNE 1209


HSP 4 Score: 51.2 bits (121), Expect = 8.9e-07
Identity = 44/154 (28.57%), Postives = 71/154 (46.10%), Query Frame = 1

Query: 4    VKELEAEEKKAEAKEKKKAEEE-----KRKEEESLRRKKEEKKALREESAKREEERRKKE 63
            +K+ E  +K+    E  K +EE     ++KE E    K  EKK   E+ +K +EE  KKE
Sbjct: 971  LKKQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREKKEYEEKKSKTKEE-AKKE 1030

Query: 64   REKKKVTRRTPISAKCLKRKKKNVESL--------VETKTKLAEIQTAKKKKKETLMDQK 123
            ++K +  +R    ++  K KK+  ES          ETK K  E +  K KKKE   + +
Sbjct: 1031 KKKSQDKKREEKDSEERKSKKEKEESRDLKAKKKEEETKEK-KESENHKSKKKEDKKEHE 1090

Query: 124  FSQEIERMSPLKPRLRPEELGLISVVQPKKGKNK 145
             ++ +++    K + + EE       + KK   K
Sbjct: 1091 DNKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEK 1122


HSP 5 Score: 48.1 bits (113), Expect = 7.5e-06
Identity = 43/154 (27.92%), Postives = 73/154 (47.40%), Query Frame = 1

Query: 2    KFVKELEAEEKKAE--AKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEER---RK 61
            K  +E + E+KK++   +E+K +EE K K+E+   R  + KK   E   K+E E    +K
Sbjct: 1021 KTKEEAKKEKKKSQDKKREEKDSEERKSKKEKEESRDLKAKKKEEETKEKKESENHKSKK 1080

Query: 62   KEREKKKVTRRTPISAKCLKRKKKNVES----LVETKTKLAEIQTA-KKKKKETLMDQKF 121
            KE +K+    ++    +  K KKK+ ES      E K  + +++     KKKE   ++K 
Sbjct: 1081 KEDKKEHEDNKSMKKEEDKKEKKKHEESKSRKKEEDKKDMEKLEDQNSNKKKEDKNEKKK 1140

Query: 122  SQE---IERMSPLKPRLRPEELGLISVVQPKKGK 143
            SQ    +++ S  K +   EE      ++  K +
Sbjct: 1141 SQHVKLVKKESDKKEKKENEEKSETKEIESSKSQ 1174


HSP 6 Score: 47.0 bits (110), Expect = 1.7e-05
Identity = 39/162 (24.07%), Postives = 75/162 (46.30%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEE--SLRRKKEEKKAL-----------REESAKRE 64
            KE   +     +K+K K +++K+KE +  ++++K+E+KK             ++E+ K E
Sbjct: 926  KEENKDTINTSSKQKGKDKKKKKKESKNSNMKKKEEDKKEYVNNELKKQEDNKKETTKSE 985

Query: 65   EERRKKEREKKKVTRRTPISAKCLKRKKKNVESLVETK---TKLAEIQTAKKKKKETLMD 124
              + K+E +  K  + +  SA   + KK+  E   +TK    K  +    KK++++   +
Sbjct: 986  NSKLKEENKDNKEKKESEDSASKNREKKEYEEKKSKTKEEAKKEKKKSQDKKREEKDSEE 1045

Query: 125  QKFSQEIERMSPLKPRLRPEELGLISVVQPKKGKNKTGALRH 151
            +K  +E E    LK + + EE       +  K K K     H
Sbjct: 1046 RKSKKEKEESRDLKAKKKEEETKEKKESENHKSKKKEDKKEH 1087


HSP 7 Score: 46.6 bits (109), Expect = 2.2e-05
Identity = 36/125 (28.80%), Postives = 66/125 (52.80%), Query Frame = 1

Query: 1    MKFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKERE 60
            M+ +++  + +KK +  EKKK++  K  ++ES    K+EKK   E+S  +E E  K   +
Sbjct: 1120 MEKLEDQNSNKKKEDKNEKKKSQHVKLVKKES---DKKEKKENEEKSETKEIESSKS--Q 1179

Query: 61   KKKVTRRTPISAK-CLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMS 120
            K +V ++   S+K   K+K+K ++   E K K  E    + +KK+T +++   Q+  +  
Sbjct: 1180 KNEVDKKEKKSSKDQQKKKEKEMKESEEKKLKKNE----EDRKKQTSVEENKKQKETKKE 1235

Query: 121  PLKPR 125
              KP+
Sbjct: 1240 KNKPK 1235


HSP 8 Score: 46.2 bits (108), Expect = 2.9e-05
Identity = 27/103 (26.21%), Postives = 58/103 (56.31%), Query Frame = 1

Query: 10   EEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALR--EESAKREEERRKKER---EKKKV 69
            E+K ++ ++KKK +E K  EE+ L++ +E++K     EE+ K++E +++K +   +KK  
Sbjct: 1182 EKKSSKDQQKKKEKEMKESEEKKLKKNEEDRKKQTSVEENKKQKETKKEKNKPKDDKKNT 1241

Query: 70   TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLM 108
            T+++    + ++ + K  E+  + K++      + + K E LM
Sbjct: 1242 TKQSGGKKESMESESKEAEN--QQKSQATTQADSDESKNEILM 1282


HSP 9 Score: 45.4 bits (106), Expect = 4.9e-05
Identity = 31/122 (25.41%), Postives = 60/122 (49.18%), Query Frame = 1

Query: 1    MKFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKERE 60
            +K VK+   +++K E +EK + +E +  + +     K+EKK+ +++  K+E+E ++ E +
Sbjct: 1144 VKLVKKESDKKEKKENEEKSETKEIESSKSQKNEVDKKEKKSSKDQQKKKEKEMKESEEK 1203

Query: 61   KKKVTRRTPISAKCLKRKKKNVESLVE-TKTKLAEIQTAKKK--KKETLMDQKFSQEIER 120
            K K           ++  KK  E+  E  K K  +  T K+   KKE++  +    E ++
Sbjct: 1204 KLKKNEEDRKKQTSVEENKKQKETKKEKNKPKDDKKNTTKQSGGKKESMESESKEAENQQ 1263


HSP 10 Score: 42.0 bits (97), Expect = 5.4e-04
Identity = 28/103 (27.18%), Postives = 52/103 (50.49%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           K +EA+ KK E+KE KK     +  E  +R K+E  +  ++ES K E+  +K+ ++ K V
Sbjct: 744 KSVEAKGKKKESKENKKT----KTNENRVRNKEENVQGNKKESEKVEKGEKKESKDAKSV 803

Query: 65  ----TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKK 104
                ++   +    + K+++ E   E K +  + Q+ + K+K
Sbjct: 804 ETKDNKKLSSTENRDEAKERSGEDNKEDKEESKDYQSVEAKEK 842


HSP 11 Score: 30.0 bits (66), Expect = 2.1e+00
Identity = 18/58 (31.03%), Postives = 30/58 (51.72%), Query Frame = 1

Query: 8    EAEEKK----AEAKEKKKAEEEKRKEEESLRRKKEEKKALRE--ESAKREEERRKKER 60
            E + KK    AE K++K+ +EEK K ++  +   E+    +E  ES  +E E ++K +
Sbjct: 1431 EEDRKKQTSVAENKKQKETKEEKNKPKDDKKNTTEQSGGKKESMESESKEAENQQKSQ 1488

BLAST of MELO3C025954T1 vs. TAIR10
Match: AT3G57150.1 (AT3G57150.1 homologue of NAP57)

HSP 1 Score: 58.5 bits (140), Expect = 5.6e-09
Identity = 39/103 (37.86%), Postives = 59/103 (57.28%), Query Frame = 1

Query: 2   KFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREK 61
           K V+  EAEEK   +K+KKK ++E+ KEEE+   KKE+KK           ++ KKE   
Sbjct: 462 KEVEGEEAEEKVKSSKKKKKKDKEEEKEEEAGSEKKEKKK-----------KKDKKEEVI 521

Query: 62  KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKE 105
           ++V   +P S K  K+K K+ E+ V+ + + A  ++ KKKKK+
Sbjct: 522 EEVA--SPKSEKKKKKKSKDTEAAVDAEDESAAEKSEKKKKKK 551

BLAST of MELO3C025954T1 vs. NCBI nr
Match: gi|918009229|ref|WP_052301815.1| (hypothetical protein [Butyrivibrio proteoclasticus])

HSP 1 Score: 79.7 bits (195), Expect = 6.6e-12
Identity = 50/131 (38.17%), Postives = 78/131 (59.54%), Query Frame = 1

Query: 4   VKELEAEEKKAEA-KEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKK 63
           +++ +AEE+K +A +EKKKAEEEK+K EE  ++ +EEKK   EE  K EEE++K E EKK
Sbjct: 372 LEKKKAEEEKKKAEEEKKKAEEEKKKAEEEKKKAEEEKKKKAEEEKKAEEEKKKAEEEKK 431

Query: 64  KVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKK----KKKETLMDQKFSQEIERM 123
           K        A+  K+K +  +   E + K AE +  KK    KKK    +QK ++E ++ 
Sbjct: 432 KAEEEEKKKAEEEKKKAEEEQKKAEEEKKKAEEEEQKKAEEEKKKAEEEEQKKAEEEKKK 491

Query: 124 SPLKPRLRPEE 130
           +  + + + EE
Sbjct: 492 AEEEEKKKVEE 502

BLAST of MELO3C025954T1 vs. NCBI nr
Match: gi|918009229|ref|WP_052301815.1| (hypothetical protein [Butyrivibrio proteoclasticus])

HSP 1 Score: 74.7 bits (182), Expect = 2.1e-10
Identity = 49/131 (37.40%), Postives = 71/131 (54.20%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           K+ E E+KKAE ++KKKAEEEK+ EEE  + ++E+KKA  EE  K EEE++K E E+KK 
Sbjct: 396 KKAEEEKKKAEEEKKKKAEEEKKAEEEKKKAEEEKKKAEEEEKKKAEEEKKKAEEEQKKA 455

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKK----KKKETLMDQKFSQEIERMSP 124
                      K+ ++  +   E + K AE +  KK    KKK    ++K  +E  +   
Sbjct: 456 EEEK-------KKAEEEEQKKAEEEKKKAEEEEQKKAEEEKKKAEEEEKKKVEEENKEES 515

Query: 125 LKPRLRPEELG 132
            K     EE G
Sbjct: 516 EKKEGEKEENG 519


HSP 2 Score: 37.0 bits (84), Expect = 4.9e+01
Identity = 24/59 (40.68%), Postives = 33/59 (55.93%), Query Frame = 1

Query: 5   KELEAEE--KKAEAKEKKKAEEEKRKEEESLRRKKEEK--------KALREESAKREEE 54
           ++ +AEE  KKAE +EKKK EEE ++E E    +KEE         +AL   +   EEE
Sbjct: 481 EQKKAEEEKKKAEEEEKKKVEEENKEESEKKEGEKEENGNSGNSAGEALEGSAGHSEEE 539


HSP 3 Score: 79.7 bits (195), Expect = 6.6e-12
Identity = 50/131 (38.17%), Postives = 78/131 (59.54%), Query Frame = 1

Query: 4   VKELEAEEKKAEA-KEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKK 63
           +++ +AEE+K +A +EKKKAEEEK+K EE  ++ +EEKK   EE  K EEE++K E EKK
Sbjct: 385 LEKKKAEEEKKKAEEEKKKAEEEKKKAEEEKKKAEEEKKKKAEEEKKAEEEKKKAEEEKK 444

Query: 64  KVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKK----KKKETLMDQKFSQEIERM 123
           K        A+  K+K +  +   E + K AE +  KK    KKK    +QK ++E ++ 
Sbjct: 445 KAEEEEKKKAEEEKKKAEEEQKKAEEEKKKAEEEEQKKAEEEKKKAEEEEQKKAEEEKKK 504

Query: 124 SPLKPRLRPEE 130
           +  + + + EE
Sbjct: 505 AEEEEKKKVEE 515

BLAST of MELO3C025954T1 vs. NCBI nr
Match: gi|302395672|gb|ADL34577.1| (GDSL-family lipase/acylhydrolase [Butyrivibrio proteoclasticus B316])

HSP 1 Score: 74.7 bits (182), Expect = 2.1e-10
Identity = 49/131 (37.40%), Postives = 71/131 (54.20%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           K+ E E+KKAE ++KKKAEEEK+ EEE  + ++E+KKA  EE  K EEE++K E E+KK 
Sbjct: 409 KKAEEEKKKAEEEKKKKAEEEKKAEEEKKKAEEEKKKAEEEEKKKAEEEKKKAEEEQKKA 468

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKK----KKKETLMDQKFSQEIERMSP 124
                      K+ ++  +   E + K AE +  KK    KKK    ++K  +E  +   
Sbjct: 469 EEEK-------KKAEEEEQKKAEEEKKKAEEEEQKKAEEEKKKAEEEEKKKVEEENKEES 528

Query: 125 LKPRLRPEELG 132
            K     EE G
Sbjct: 529 EKKEGEKEENG 532


HSP 2 Score: 37.0 bits (84), Expect = 4.9e+01
Identity = 24/59 (40.68%), Postives = 33/59 (55.93%), Query Frame = 1

Query: 5   KELEAEE--KKAEAKEKKKAEEEKRKEEESLRRKKEEK--------KALREESAKREEE 54
           ++ +AEE  KKAE +EKKK EEE ++E E    +KEE         +AL   +   EEE
Sbjct: 494 EQKKAEEEKKKAEEEEKKKVEEENKEESEKKEGEKEENGNSGNSAGEALEGSAGHSEEE 552


HSP 3 Score: 77.4 bits (189), Expect = 3.3e-11
Identity = 49/142 (34.51%), Postives = 77/142 (54.23%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           K    E ++A+A+EKKKAEE +R + E  ++ +E +KA  EE  K EE  R K  EKKK 
Sbjct: 359 KRAAEERERAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKKA 418

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAK--KKKKETLMDQKFSQEIERMSPLK 124
             R    A+  K+ ++   +  E K K  E + AK  +KKK    ++  ++E +R +  K
Sbjct: 419 EEREKAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKRAAEEK 478

Query: 125 PRLRPEELGLISVVQPKKGKNK 145
            +L  E+L     V+ KK ++K
Sbjct: 479 AKLEEEKLKAKQKVEEKKIEDK 500

BLAST of MELO3C025954T1 vs. NCBI nr
Match: gi|465978720|gb|EMP35047.1| (Caldesmon [Chelonia mydas])

HSP 1 Score: 68.9 bits (167), Expect = 1.2e-08
Identity = 55/162 (33.95%), Postives = 79/162 (48.77%), Query Frame = 1

Query: 8   EAEEKK--AEAKEKKKAEEEKRKEEESLRRKKEEKK-------ALREESAKREEERRKKE 67
           +AEE+K  AE +E+ KAEEEKR  EE  R K EEKK       A  EE  K EE  + K 
Sbjct: 339 KAEEEKRAAEERERAKAEEEKRAAEERERAKAEEKKKAEERERAKAEEKKKAEEREKAKA 398

Query: 68  REKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAK----------------KKK 127
            EKKK   R    A+  K+ ++  ++  E K K  E + AK                +KK
Sbjct: 399 EEKKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKK 458

Query: 128 KETLMDQKFSQEIERMSPLKPRLRPEELGLISVVQPKKGKNK 145
           K    ++  ++E +R +  K +L  E+L     V+ KK ++K
Sbjct: 459 KAEERERAKAEEKKRAAEEKAKLEEEKLKAKQKVEEKKIEDK 500


HSP 2 Score: 68.2 bits (165), Expect = 2.0e-08
Identity = 53/171 (30.99%), Postives = 85/171 (49.71%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           K+   E +KA+A+EKKKAEE +R + E  ++ +E +KA  EE  K EE  R K  EKKK 
Sbjct: 387 KKKAEEREKAKAEEKKKAEERERAKAEEKKKAEEREKAKAEEKKKAEERERAKAEEKKKA 446

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAE----IQTAKKKKKETLMDQKFSQEIERMSP 124
             R    A+  K+ ++   +  E K + AE    ++  K K K+ + ++K   +  +   
Sbjct: 447 EEREKAKAEEKKKAEERERAKAEEKKRAAEEKAKLEEEKLKAKQKVEEKKIEDKKVQDKQ 506

Query: 125 LKPRLRPEELGLISVVQPKKGKNKTGALRHLGRLLNLLYEPIK-ALKSEKI 171
           +K +   E+      VQ +  K +T +LR  G    +  E  K  L  EK+
Sbjct: 507 VKEKKIEEKQVKGKKVQEE--KPQTVSLRKQGEEKEVKVEAKKEKLPDEKL 555


HSP 3 Score: 52.0 bits (123), Expect = 1.5e-03
Identity = 41/146 (28.08%), Postives = 77/146 (52.74%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKE-EESLRRKKEEKKALREESAK--REEERRKKEREK 64
           ++L  EE++   +EKK+ EE +R E EE  R K EE+K + EE A+   EE+R  +E+E+
Sbjct: 256 EKLRNEEEEKTEEEKKRTEERERLEAEERERVKAEEQKKVEEERARIEAEEKRAAQEKER 315

Query: 65  KKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPL 124
            +   R  I A+  +  ++   +  E + + AE +   K ++E    ++ ++E ER +  
Sbjct: 316 IEAEERERIQAEQQRAAEERERAKAEEEKRAAEERERAKAEEE----KRAAEERER-AKA 375

Query: 125 KPRLRPEELGLISVVQPKKGKNKTGA 148
           + + + EE       + KK + +  A
Sbjct: 376 EEKKKAEERERAKAEEKKKAEEREKA 396


HSP 4 Score: 38.9 bits (89), Expect = 1.3e+01
Identity = 42/179 (23.46%), Postives = 82/179 (45.81%), Query Frame = 1

Query: 1   MKFVKELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKERE 60
           MK     E  +  ++A+  K+ EE +R+  E+   + E+ K  ++E+A   EE +KK  E
Sbjct: 620 MKATTNAEDGKPVSQAEAGKRLEELRRRRGENESEEFEKLKEKQQEAAVELEELKKKREE 679

Query: 61  KKKVTRRTPISAKCLKRKKKNVESLVETKTKLAE------IQTAKKKKK---ETLMDQKF 120
           ++K+        K  + ++K  E   E K +L E       + A+K++K   + L D K 
Sbjct: 680 RRKILEEEEQRRKQEEAERKAREE--EEKRRLKEDIERRRAEAAEKRQKMPEDGLSDDK- 739

Query: 121 SQEIERMSPLKPRLRPEELG-LISVVQPKKGKNKTGALRHLGRLLNLLYEPIKALKSEK 170
            +  +  +P    L+ EE    ++    K G   T +   + ++ + L +   A++  K
Sbjct: 740 -KPFKCFTPKGSSLKIEERAEFLNKSAQKSGAKPTHSAAIVSKIDSRLEQYTSAIEGTK 794


HSP 5 Score: 75.5 bits (184), Expect = 1.2e-10
Identity = 44/120 (36.67%), Postives = 70/120 (58.33%), Query Frame = 1

Query: 5   KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKKEEKKALREESAKREEERRKKEREKKKV 64
           +E +  ++K +A+EKKKAEEEK+K +E  ++ +EEKK + EE  K EEE++K E  KK  
Sbjct: 774 EEAKKADEKKKAEEKKKAEEEKKKADEEKKKAEEEKKKVEEEKKKVEEEKKKAEEVKKVE 833

Query: 65  TRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMSPLKPR 124
            ++     K  + KKK      ETK   A  +  KKK+K+T  D K  ++ ++  P + +
Sbjct: 834 EKKKEEEKKKAEEKKKE----EETKKDHANNKGKKKKEKKTGDDAKHDEKKKKKKPTEAK 889

BLAST of MELO3C025954T1 vs. NCBI nr
Match: gi|67478965|ref|XP_654864.1| (hypothetical protein [Entamoeba histolytica HM-1:IMSS])

HSP 1 Score: 75.1 bits (183), Expect = 1.6e-10
Identity = 45/130 (34.62%), Postives = 77/130 (59.23%), Query Frame = 1

Query: 5    KELEAEEKKAEAKEKKKAEEEKRKEEESLRRKK---EEKKALREESAKREEERRKK--ER 64
            K  E EE+K + +EK+KAEEE++++EE LR+KK   E+K+ L EE  K+EEE RKK  E 
Sbjct: 1109 KRKEEEERKRKEEEKRKAEEERKRKEEELRKKKEAEEKKRKLEEEHKKKEEELRKKKEEE 1168

Query: 65   EKKKVTRRTPISAKCLKRKKKNVESLVETKTKLAEIQTAKKKKKETLMDQKFSQEIERMS 124
            EK++       + +  KRK++  ++  E + ++   +  +KK++E    +K  +E+    
Sbjct: 1169 EKRRQEEEKRKAEEERKRKEEEEKARKEEEERIKREEEERKKQEEEERKKKEEEELRVKQ 1228

Query: 125  PLKPRLRPEE 130
              + + R EE
Sbjct: 1229 EEEKKKRAEE 1238

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CALD1_CHICK2.5e-1132.77Caldesmon OS=Gallus gallus GN=CALD1 PE=1 SV=2[more]
NST1_VANPO4.3e-1133.80Stress response protein NST1 OS=Vanderwaltozyma polyspora (strain ATCC 22028 / D... [more]
NST1_KLULA1.2e-1036.64Stress response protein NST1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 235... [more]
NST1_EMENI2.1e-1029.32Stress response protein nst1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163... [more]
NST1_CANAL2.8e-1038.89Stress response protein NST1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876)... [more]
Match NameE-valueIdentityDescription
E0RWQ1_BUTPB4.6e-1238.17GDSL-family lipase/acylhydrolase OS=Butyrivibrio proteoclasticus (strain ATCC 51... [more]
E0RWQ1_BUTPB1.5e-1037.40GDSL-family lipase/acylhydrolase OS=Butyrivibrio proteoclasticus (strain ATCC 51... [more]
M7C3L9_CHEMY3.6e-0933.13Caldesmon OS=Chelonia mydas GN=UY3_07777 PE=4 SV=1[more]
A2FHD4_TRIVA1.5e-0733.64Trichohyalin, putative OS=Trichomonas vaginalis GN=TVAG_144410 PE=4 SV=1[more]
M3UWV9_ENTHI2.5e-1034.88IgA-specific serine endopeptidase, putative (Fragment) OS=Entamoeba histolytica ... [more]
Match NameE-valueIdentityDescription
AT2G18540.17.7e-1131.78 RmlC-like cupins superfamily protein[more]
AT5G60030.15.0e-1030.57 unknown protein[more]
AT1G29000.18.6e-1041.89 Heavy metal transport/detoxification superfamily protein [more]
AT3G28770.11.5e-0929.17 Protein of unknown function (DUF1216)[more]
AT3G57150.15.6e-0937.86 homologue of NAP57[more]
Match NameE-valueIdentityDescription
gi|918009229|ref|WP_052301815.1|6.6e-1238.17hypothetical protein [Butyrivibrio proteoclasticus][more]
gi|918009229|ref|WP_052301815.1|2.1e-1037.40hypothetical protein [Butyrivibrio proteoclasticus][more]
gi|302395672|gb|ADL34577.1|2.1e-1037.40GDSL-family lipase/acylhydrolase [Butyrivibrio proteoclasticus B316][more]
gi|465978720|gb|EMP35047.1|1.2e-0833.95Caldesmon [Chelonia mydas][more]
gi|67478965|ref|XP_654864.1|1.6e-1034.62hypothetical protein [Entamoeba histolytica HM-1:IMSS][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MELO3C025954MELO3C025954gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MELO3C025954T1MELO3C025954T1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MELO3C025954T1.cds2MELO3C025954T1.cds2CDS
MELO3C025954T1.cds1MELO3C025954T1.cds1CDS


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableunknownCoilCoilcoord: 4..68
scor