Bhi01G000326 (gene) Wax gourd

NameBhi01G000326
Typegene
OrganismBenincasa hispida (Wax gourd)
Descriptionjasmonic acid-amido synthetase JAR1
Locationchr1 : 7885067 .. 7888936 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAATCTTCTATCGCATCCACATCCTTGTCTCTCTCCAGTCTCCATTGAAGAAGCCAAGTAAAAAGCTCTGTAACTCTCTCTCTCTCTCTAGAAACTTTCCAAGAGGAGGAGGGGAGAGAGAAAGATGATCTTACTTTACTAATTGTATCTGTAGATTTTTTTATGATTCTTCTCTCTTTGTAGCGCTCATACACTTGTCCGCTTAACTGAAGTTGCTGCGGACTGTGCGGAACTATTTCTTGCATCACTTTCAACTAGTTCCAAATTCGATTTCAGGTAATTTCTATACCGGACTTTGATTTCGGGTTTCCATTTCTGTAAGGGCAAGAGTTTTTTCGAGGTGGAATTGATGCGAATACGGCACTGTTTGTTGATTGCCGTTCAGGTTTAACTATAATTTGGAAACGTTTGTTTTCAATTTCTTTTCATGATTTGATTTCGTGGTCGAATCCATCGTCATACTCTGTTCTTATTCCGTTTTCGTCTTTCTCAGTGTTTCTTAATCGTCGTTGACGAGCTCCGTAAAATTTTAATTTAATTTTTATATTTGAATTGTGGTTTATATATATATATATATTTAAAAAAAGCCATTTGTTCGGTTCTGGAAACGGTTTAATACTAAAAACCCGACTGAGTTGGCGTCCTTGTTTCTTCTCTATTTTCCGGGAAACAGTTTCCCGGAAAATTTCTTGATAGTTGACTTGGTTGAATTCCATCGGATAATTTAGATCTTCCACCGCTGTGCTCTGAGGGTAGTTCAATCGTATCAATCAGTTCCACTTGCTGTAATTGCTTTTACTTAAAAGTTGCTGTTCAATTGTTGACTTAGCAACGAGTTTGCCATATTCTCTCTTACTTTTTCGTTTTCCTTTTTGGGTTTTGCAAAAAAGATCAATCCCCAAATCAAATTTGCGGGACACTTGTCCTTCATCTTATCTCATGGACCCCTGCCGTTATTCACGTTTTTGGGTTTTACCTTTTTCCTTTGCCTTATCGAGCAGTTTCGAGTCCTTTACTGTCTAATAAATTTGATGGGTTTCATTTTAAGCTAATCTTCGTTATACTCCGTTGGTCTTTGTTTGGAACAGTCTCTGACCGACGAGAATGTTGGAGAAAATGGAAGCATTTGATGGGGAAAAAGTGATAGAGGAATTTGAGGAAATGACCAAAGATGCTGAAAGAGTTCAGAGGGAAACTCTGAAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAACTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCGCTTGTCACTCACGATGATTTGGAGTCATATATTCAGAGAATCGCGGATGGGGATTCTTCCCCTATTCTCACTGGAAAACCAATCAAAACCATTTCGTTGAGGTACTTTTGCATATTTGTTCTGAAACAAGCTTCGTTTTGATTCATAGTATTGGAGATTTTTGTTGTTGTTGTAGTTGTTTGCTCTAAGGAGACTTTTGGTTGGTTATTGTAGCTCTGGTACTACTAAAGGGAGGCCCAAGTTAATTCCCTTCAACGATGAATTGTTGGAGACCACAATGCAAATATATCGCACTTCTTTTGCCTTCAGAAACAAGTAATGAACCTGTCTTTTCTAGTTTATCTTTTGAGGAATCTTTTATGTCCTGTGTTCGGAATTGTTTGTGGTTTTGTCTCTGAACCTTTTGTTGTCTGAACTGAACTGCTTAATTGTTCAGTTTGTTGTCTTTATGCTGTCTCTTTTATCATCTTATTGAGTACTAAATAGTGTAAAATGGGTGTTTGTCATGGCTGATGTGATGAGAGTACTTTGTTCTACAGAGAAGTTCCCATTAGGAATGGAAAAGCCTTGCAGTTCATCTACAGCAGCAAGGCGCTCAAAACTGGTGGTGGTCTGGCAGCAGGAACTGCAACGACGAACGTTTACCGCAGTGCAAAATTTAAGAGCACGATGAAAGCAATCCAGTCGCAATGCTGTAGCCCAGACGAAGTCATCTTTGGCCCTGACTTCCACCAATCTTTGTATTGCCATCTCTTGTGTGGGCTAATCTTCCGGGATGAAGTTGAGTTTGTGTTCTCCACTTTTGCACACAGCATTGTCCATTCTTTCAGGACTTTTGAGCAAGTATGGGAAGAGCTATGCAGCAACATCCGAGATGGTGTTCTCTCTAGTTGGGTCACCGCCCCTTCCATTCGTGGGGCGATGTCGAAATTGCTTAAACCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGTGCAGGATTGAGTAATTGGTATGGGCTGATACCAGAGCTCTTTCCCAATGCAAAGTACATTTATGGTATCATGACTGGCTCGATGGAGCCTTACCTAAAAAAACTGAGGCACTATGGGGGCCATTTGCCACTGATGAGTGCTGATTATGGTTCTTCGGAAGGGTGGGTTGGAGCAAACGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTACTGTGCTTCCAAACATCGGATACTTCGAATTCATCCCGCTAAAGGAGAATGCTGAAGATCAGCAGCAGGAGAACAAGCCGATTGGTCTGACTGAAGTCAAGATAGGTGAAGAGTATGAAATAATTGTCACAAATGTTGCAGGTAATTTCTCTTAATCCATGACTTTAGATGCAATAACATTGTTTTGTTACTTATAAGTCAAACATGAACGAACAGGAAACTGAGTGACAGTTGTTTTCCCATAATATCATATTGAACTCACGGTCCATCTCCACATATGCTATGTTGTTTGGTTATTCAAATGTCAAACTTGTTTCTATGCATCCCATGTGACTGTTGGGGGGTCTGCACTGCAGGAACGATCTCTGCCCCTTGCTTTGTCCTGTTCTATCTCCTACATGTTTCTCTGTTATGTTGTGGGGTCGTCTTATATATTGTCCACTTTAAACCTAACCCCACAGTGAATTCAAATTTACTTTCAAGACACTAACAAAAAAACATTAATCGCGATTGTTGCAGGGCTGTACCGTTATAGATTAGGGGACGTGGTGAAAGTAATAGGGTTCCACAACTCGACACCAAAGCTGAAATTCATATGCCGGAGGAACCTACTATTGACCATCAATATCGACAAGAACACAGAGAAGGACCTGCAGCTAGCAGTGGAGGCAGGCGGGAACGTGCTGGCAGCAGAGAAGCTAGAAGTGGTGGACTTCACAAGCTATGTCGACGTATCGAGGGAGCCGGGGCACTACGTGATATTCTGGGAGATAAGTGGAGAGGCAAAAGGGGAGGTGCTGGGGGAATGCTCCAACTGTATGGACAGGGCTTTCCTAGACGCGGGGTACATGAGCTCAAGAAAGGTTAATGCCATTGGAGCCCTTGAGCTGAGAGTGGTTCGTAAGGGAACTTTTCACAAGATTATGGATCATTATCTGTCGTTAGGAGCGGCTGTGAGTCAGTACAAAACTCCTCGTTGTGTTAACCCCACAAACACCACTGTGTTGCAGATCTTGTGCTCCAATGTCGTTAACTCCTATTTTAGTACTGCCTTCTAGTTTTTTTGTTTTCCTCATCTCTCTTCTTTTTTTTTTTCCCCATTACCCATGTGCTCTCTTTCTCTGCTTTATAATGTATATTTCAAATTCTAGTTATAAACTTTTCCTTTTTTTAATTGGTTCCCCATAAACAAACAATCACTTGTCAGCTTGGATATAATTTAATGGATAGGGTACATGCCTTAATTGTTAAGCTAACTTTGGTACTTATC

mRNA sequence

GGAATCTTCTATCGCATCCACATCCTTGTCTCTCTCCAGTCTCCATTGAAGAAGCCAACGCTCATACACTTGTCCGCTTAACTGAAGTTGCTGCGGACTGTGCGGAACTATTTCTTGCATCACTTTCAACTAGTTCCAAATTCGATTTCAGTCTCTGACCGACGAGAATGTTGGAGAAAATGGAAGCATTTGATGGGGAAAAAGTGATAGAGGAATTTGAGGAAATGACCAAAGATGCTGAAAGAGTTCAGAGGGAAACTCTGAAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAACTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCGCTTGTCACTCACGATGATTTGGAGTCATATATTCAGAGAATCGCGGATGGGGATTCTTCCCCTATTCTCACTGGAAAACCAATCAAAACCATTTCGTTGAGCTCTGGTACTACTAAAGGGAGGCCCAAGTTAATTCCCTTCAACGATGAATTGTTGGAGACCACAATGCAAATATATCGCACTTCTTTTGCCTTCAGAAACAAAGAAGTTCCCATTAGGAATGGAAAAGCCTTGCAGTTCATCTACAGCAGCAAGGCGCTCAAAACTGGTGGTGGTCTGGCAGCAGGAACTGCAACGACGAACGTTTACCGCAGTGCAAAATTTAAGAGCACGATGAAAGCAATCCAGTCGCAATGCTGTAGCCCAGACGAAGTCATCTTTGGCCCTGACTTCCACCAATCTTTGTATTGCCATCTCTTGTGTGGGCTAATCTTCCGGGATGAAGTTGAGTTTGTGTTCTCCACTTTTGCACACAGCATTGTCCATTCTTTCAGGACTTTTGAGCAAGTATGGGAAGAGCTATGCAGCAACATCCGAGATGGTGTTCTCTCTAGTTGGGTCACCGCCCCTTCCATTCGTGGGGCGATGTCGAAATTGCTTAAACCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGTGCAGGATTGAGTAATTGGTATGGGCTGATACCAGAGCTCTTTCCCAATGCAAAGTACATTTATGGTATCATGACTGGCTCGATGGAGCCTTACCTAAAAAAACTGAGGCACTATGGGGGCCATTTGCCACTGATGAGTGCTGATTATGGTTCTTCGGAAGGGTGGGTTGGAGCAAACGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTACTGTGCTTCCAAACATCGGATACTTCGAATTCATCCCGCTAAAGGAGAATGCTGAAGATCAGCAGCAGGAGAACAAGCCGATTGGTCTGACTGAAGTCAAGATAGGTGAAGAGTATGAAATAATTGTCACAAATGTTGCAGGGCTGTACCGTTATAGATTAGGGGACGTGGTGAAAGTAATAGGGTTCCACAACTCGACACCAAAGCTGAAATTCATATGCCGGAGGAACCTACTATTGACCATCAATATCGACAAGAACACAGAGAAGGACCTGCAGCTAGCAGTGGAGGCAGGCGGGAACGTGCTGGCAGCAGAGAAGCTAGAAGTGGTGGACTTCACAAGCTATGTCGACGTATCGAGGGAGCCGGGGCACTACGTGATATTCTGGGAGATAAGTGGAGAGGCAAAAGGGGAGGTGCTGGGGGAATGCTCCAACTGTATGGACAGGGCTTTCCTAGACGCGGGGTACATGAGCTCAAGAAAGGTTAATGCCATTGGAGCCCTTGAGCTGAGAGTGGTTCGTAAGGGAACTTTTCACAAGATTATGGATCATTATCTGTCGTTAGGAGCGGCTGTGAGTCAGTACAAAACTCCTCGTTGTGTTAACCCCACAAACACCACTGTGTTGCAGATCTTGTGCTCCAATGTCGTTAACTCCTATTTTAGTACTGCCTTCTAGTTTTTTTGTTTTCCTCATCTCTCTTCTTTTTTTTTTTCCCCATTACCCATGTGCTCTCTTTCTCTGCTTTATAATGTATATTTCAAATTCTAGTTATAAACTTTTCCTTTTTTTAATTGGTTCCCCATAAACAAACAATCACTTGTCAGCTTGGATATAATTTAATGGATAGGGTACATGCCTTAATTGTTAAGCTAACTTTGGTACTTATC

Coding sequence (CDS)

ATGTTGGAGAAAATGGAAGCATTTGATGGGGAAAAAGTGATAGAGGAATTTGAGGAAATGACCAAAGATGCTGAAAGAGTTCAGAGGGAAACTCTGAAGAAGATTTTGGAGGAAAATGGGTCGGCTGAGTACTTGCAGAACTTGGGACTTAATGGAAGAACTGATCCTCAGAGCTTCAAGGACTGTGTCCCGCTTGTCACTCACGATGATTTGGAGTCATATATTCAGAGAATCGCGGATGGGGATTCTTCCCCTATTCTCACTGGAAAACCAATCAAAACCATTTCGTTGAGCTCTGGTACTACTAAAGGGAGGCCCAAGTTAATTCCCTTCAACGATGAATTGTTGGAGACCACAATGCAAATATATCGCACTTCTTTTGCCTTCAGAAACAAAGAAGTTCCCATTAGGAATGGAAAAGCCTTGCAGTTCATCTACAGCAGCAAGGCGCTCAAAACTGGTGGTGGTCTGGCAGCAGGAACTGCAACGACGAACGTTTACCGCAGTGCAAAATTTAAGAGCACGATGAAAGCAATCCAGTCGCAATGCTGTAGCCCAGACGAAGTCATCTTTGGCCCTGACTTCCACCAATCTTTGTATTGCCATCTCTTGTGTGGGCTAATCTTCCGGGATGAAGTTGAGTTTGTGTTCTCCACTTTTGCACACAGCATTGTCCATTCTTTCAGGACTTTTGAGCAAGTATGGGAAGAGCTATGCAGCAACATCCGAGATGGTGTTCTCTCTAGTTGGGTCACCGCCCCTTCCATTCGTGGGGCGATGTCGAAATTGCTTAAACCAAATCCTGAATTGGCAGATTTGATCTATCGAAAATGTGCAGGATTGAGTAATTGGTATGGGCTGATACCAGAGCTCTTTCCCAATGCAAAGTACATTTATGGTATCATGACTGGCTCGATGGAGCCTTACCTAAAAAAACTGAGGCACTATGGGGGCCATTTGCCACTGATGAGTGCTGATTATGGTTCTTCGGAAGGGTGGGTTGGAGCAAACGTTAACCCAATGTTGCCCCCTGAAATGGCCACCTTTACTGTGCTTCCAAACATCGGATACTTCGAATTCATCCCGCTAAAGGAGAATGCTGAAGATCAGCAGCAGGAGAACAAGCCGATTGGTCTGACTGAAGTCAAGATAGGTGAAGAGTATGAAATAATTGTCACAAATGTTGCAGGGCTGTACCGTTATAGATTAGGGGACGTGGTGAAAGTAATAGGGTTCCACAACTCGACACCAAAGCTGAAATTCATATGCCGGAGGAACCTACTATTGACCATCAATATCGACAAGAACACAGAGAAGGACCTGCAGCTAGCAGTGGAGGCAGGCGGGAACGTGCTGGCAGCAGAGAAGCTAGAAGTGGTGGACTTCACAAGCTATGTCGACGTATCGAGGGAGCCGGGGCACTACGTGATATTCTGGGAGATAAGTGGAGAGGCAAAAGGGGAGGTGCTGGGGGAATGCTCCAACTGTATGGACAGGGCTTTCCTAGACGCGGGGTACATGAGCTCAAGAAAGGTTAATGCCATTGGAGCCCTTGAGCTGAGAGTGGTTCGTAAGGGAACTTTTCACAAGATTATGGATCATTATCTGTCGTTAGGAGCGGCTGTGAGTCAGTACAAAACTCCTCGTTGTGTTAACCCCACAAACACCACTGTGTTGCAGATCTTGTGCTCCAATGTCGTTAACTCCTATTTTAGTACTGCCTTCTAG

Protein sequence

MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFKDCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTMQIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCSNIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEFIPLKENAEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF
BLAST of Bhi01G000326 vs. TAIR10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein)

HSP 1 Score: 818.1 bits (2112), Expect = 3.3e-237
Identity = 390/578 (67.47%), Postives = 479/578 (82.87%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGR-TDP-QS 60
           MLEK+E FD  +VI+EF+EMT++A +VQ++TLK+IL +N SA YLQN GLNG  TDP ++
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 71

Query: 61  FKDCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLET 120
           FK  VPLVT  +LE YI+R+ DGD+SPILTG P+  ISLSSGT++GRPK IPF DEL+E 
Sbjct: 72  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 131

Query: 121 TMQIYRTSFAFRNKEVPI-RNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMK 180
           T+Q++RT+FAFRN++ PI  NGKALQFI+SSK   + GG+  GTATTNVYR+  FK+ MK
Sbjct: 132 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 191

Query: 181 AIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEE 240
           +I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VH+FRTFEQVWEE
Sbjct: 192 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 251

Query: 241 LCSNIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           + ++I+DGVLS+ +T PS+R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPNAKY
Sbjct: 252 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 311

Query: 301 IYGIMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGY 360
           +YGIMTGSMEPY+ KLRHY G LPL+S DYGSSEGW+ ANV P L PE ATF V+PN+GY
Sbjct: 312 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 371

Query: 361 FEFIPLKENAEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTP 420
           FEF+P+ E  E    E KP+GLT+VKIGEEYE+++TN AGLYRYRLGDVVKVIGF+N+TP
Sbjct: 372 FEFLPVSETGEG---EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTP 431

Query: 421 KLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVI 480
           +LKFICRRNL+L+INIDKNTE+DLQL+VE+    L+ EK+EV+DF+SY+DVS +PGHY I
Sbjct: 432 QLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYAI 491

Query: 481 FWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLS 540
           FWEISGE   +VL +C NC+DRAF+DAGY+SSRK   IGALELRVV KGTF KI +H+L 
Sbjct: 492 FWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLG 551

Query: 541 LGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           LG++  Q+K PRCV P+N  VLQILC NVV+SYFSTAF
Sbjct: 552 LGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

BLAST of Bhi01G000326 vs. TAIR10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 577.4 bits (1487), Expect = 9.8e-165
Identity = 302/593 (50.93%), Postives = 407/593 (68.63%), Query Frame = 0

Query: 2   LEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQN-LG------LNGRT 61
           +E +EA   + VI  FE ++++A +VQ ETL++ILE N   EYL+  LG      ++  T
Sbjct: 1   METVEA-GHDDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKMDDYT 60

Query: 62  DPQSFKDCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDE 121
               F   VP+V+H DL+ YIQRIADG++SP+LT +PI  +SLSSGTT+GR K +PF   
Sbjct: 61  LETLFTSLVPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRH 120

Query: 122 LLETTMQIYRTSFAFRNKEVPIR-NGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFK 181
             +TT+QI+R S A+R++  PIR  G+ L+FIY+ K  KT GGL  GTATT+ Y S +FK
Sbjct: 121 SAQTTLQIFRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFK 180

Query: 182 STMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQ 241
           +  +  +S  CSP EVI G DF Q  YCHLL GL +  +VEFV S F+++IV +F  FE+
Sbjct: 181 TKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEE 240

Query: 242 VWEELCSNIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLS---NWYGLIPE 301
           +W E+C++I++G LSS +T P +R A+  L++PNP LA  I   C  L     W+GLI +
Sbjct: 241 IWREICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISK 300

Query: 302 LFPNAKYIYGIMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFT 361
           L+PNAK+I  IMTGSM PYL KLRHY G LPL+SADYGS+E W+G NV+P LPPE  +F 
Sbjct: 301 LWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFA 360

Query: 362 VLPNIGYFEFIPL--KENAED-----QQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRL 421
           V+P   YFEFIPL  ++N  D        E+KP+ L++VK+G+EYE+++T   GLYRYRL
Sbjct: 361 VIPTFSYFEFIPLYRRQNQSDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRL 420

Query: 422 GDVVKVIGFHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLA-AEKLEVVDF 481
           GDVV+V  FH  TPKL FI RR L+LTINIDKNTEKDLQ  V+    +L+ + + EVVDF
Sbjct: 421 GDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDF 480

Query: 482 TSYVDVSREPGHYVIFWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRV 541
           TS+ DV   PGHYVI+WEI GEA  + L EC   MD AF+D GY+ SR++N+IG LELRV
Sbjct: 481 TSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRV 540

Query: 542 VRKGTFHKIMDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           V +GTF K+ +  +     ++Q+KTPRC   TN+ +L IL  + +  + S+A+
Sbjct: 541 VERGTFGKVAERCVGKCGGLNQFKTPRCT--TNSVMLDILNDSTIKRFRSSAY 590

BLAST of Bhi01G000326 vs. TAIR10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 434.1 bits (1115), Expect = 1.3e-121
Identity = 230/571 (40.28%), Postives = 355/571 (62.17%), Query Frame = 0

Query: 12  KVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFKDCVPLVTHDDL 71
           K +   EEMT++ + VQ++ +++IL  N   EYL+  GL G TD ++FK  VP+V +DDL
Sbjct: 21  KALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIYDDL 80

Query: 72  ESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTMQIYRTSFAFRN 131
           +  IQRIA+GD S IL+  PI     SSGT+ G  KL+P  DE ++    +Y       N
Sbjct: 81  KPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMPVMN 140

Query: 132 KEVP-IRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQSQCCSPDEVI 191
             VP +  GKAL F++     KT GGL A    T+ Y+S +FK       +   SP+E I
Sbjct: 141 LYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPNEAI 200

Query: 192 FGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCSNIRDGVLSSW 251
             PD  QS+Y  +LCGL+ R EV  + + FA  ++ +    +  W+EL  +I  G LSS 
Sbjct: 201 LCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTLSSR 260

Query: 252 VTAPSIRGAMSKLL-KPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPY 311
           ++ P+I+ +MSK+L KP+ ELAD I   C   ++W G+I +++PN KY+  I+TG+M  Y
Sbjct: 261 ISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAMAQY 320

Query: 312 LKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEFIPLKENAED 371
           +  L +Y G LP+    Y SSE + G N+ PM  P   ++T++PN+ YFEF+P   + E 
Sbjct: 321 IPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP---HHEV 380

Query: 372 QQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLKFICRRNLLL 431
             ++++ + L +V++G+EYE+++T  AGL RYR+GD+++V GF+NS P+ KF+ R+N+LL
Sbjct: 381 PTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVRRKNVLL 440

Query: 432 TINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWEI-----SGE 491
           +I  DK  E +LQ AVE    +L  +   V+++TSY +    PGHYVI+WE+     +  
Sbjct: 441 SIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVKDQTNP 500

Query: 492 AKGEVLGECSNCMDRAFLDAGYMSSRKVN-AIGALELRVVRKGTFHKIMDHYLSLGAAVS 551
              EV+  C   M+ + L++ Y  SR  + +IG LE+RVV+ GTF ++MD+ +S GA+++
Sbjct: 501 PNDEVMARCCLEMEES-LNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISRGASIN 560

Query: 552 QYKTPRCVNPTNTTVLQILCSNVVNSYFSTA 575
           QYK PRCV  + T ++++L S VV+++FS A
Sbjct: 561 QYKVPRCV--SFTPIMELLDSRVVSTHFSPA 585

BLAST of Bhi01G000326 vs. TAIR10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 432.6 bits (1111), Expect = 3.9e-121
Identity = 237/569 (41.65%), Postives = 340/569 (59.75%), Query Frame = 0

Query: 12  KVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFKDCVPLVTHDDL 71
           K +   EEMT++A+ VQ   L +IL  N   EYL+   L G TD  +FK  +P++T++DL
Sbjct: 21  KALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVITYEDL 80

Query: 72  ESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTMQIYRTSFAFRN 131
           +  IQRIADGD SPIL+  PI     SSGT+ G  KL+P   E L+    +Y       N
Sbjct: 81  QPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMN 140

Query: 132 KEVP-IRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQSQCCSPDEVI 191
             VP +  GK + F++     KT GGL A    T+ Y+S  F+S      +   SP+E I
Sbjct: 141 LYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSPNEAI 200

Query: 192 FGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCSNIRDGVLSSW 251
             PD  QS+Y  +LCGL+ R  V  V + FA  ++ + R  +  W     +I  G L S 
Sbjct: 201 LCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGCLDSE 260

Query: 252 VTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYL 311
           +T PSIR  MS +LKP+P LA+ I R+C    NW  +I  ++PN KY+  I+TG+M  Y+
Sbjct: 261 ITDPSIRQCMSGILKPDPVLAEFIRRECKS-DNWEKIITRIWPNTKYLDVIVTGAMAQYI 320

Query: 312 KKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEFIPLKENAEDQ 371
             L +Y G LP+    Y SSE + G N+NPM  P   ++T++PN+ YFEFIPL       
Sbjct: 321 PTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPL------- 380

Query: 372 QQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLKFICRRNLLLT 431
               K + L +V IG+EYE++VT  AGL RYR+GD+++V GFHNS P+  F+ R+N+LL+
Sbjct: 381 -GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKNVLLS 440

Query: 432 INIDKNTEKDLQLAVEAGGNVLAAE-KLEVVDFTSYVDVSREPGHYVIFWEI-----SGE 491
           I+ DK  E +LQ AVE   ++L  E    V ++TSY D S  PGHYV++WE+     + +
Sbjct: 441 IDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDGARQ 500

Query: 492 AKGEVLGECSNCMDRAFLDAGYMSSRKV-NAIGALELRVVRKGTFHKIMDHYLSLGAAVS 551
              E L  C   M+ + L++ Y  SR   N++G LE+RVVR GTF ++MD+ +S GA+++
Sbjct: 501 PSHETLTRCCLGMEES-LNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGASIN 560

Query: 552 QYKTPRCVNPTNTTVLQILCSNVVNSYFS 573
           QYK PRCVN   T ++++L S VV+++FS
Sbjct: 561 QYKVPRCVN--FTPIVELLDSRVVSAHFS 577

BLAST of Bhi01G000326 vs. TAIR10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 432.6 bits (1111), Expect = 3.9e-121
Identity = 231/574 (40.24%), Postives = 350/574 (60.98%), Query Frame = 0

Query: 12  KVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFKDCVPLVTHDDL 71
           K ++  EEMT++ + VQ + L +IL  N + EYL+   L+G  D ++FK  VP+VT++DL
Sbjct: 22  KALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVTYEDL 81

Query: 72  ESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTMQIYRTSFAFRN 131
           +  IQRI++GD SPIL+  PI     SSGT+ G  KL+P  +E L+    +Y       N
Sbjct: 82  KPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMPVMN 141

Query: 132 KEVP-IRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQSQCCSPDEVI 191
             VP +  GK L F++     KT GGL A    T+ Y+S  FK       +   SP+E I
Sbjct: 142 LYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPNEAI 201

Query: 192 FGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCSNIRDGVLSSW 251
              D  QS+Y  +LCGL+ R EV  + + FA  ++ +    +  W+EL  +I  G LSS 
Sbjct: 202 LCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTLSSR 261

Query: 252 VTAPSIRGAMSKLL-KPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPY 311
           +  P+I+  MSK+L KP+ ELA+ +   C+   NW G+I +++PN KY+  I+TG+M  Y
Sbjct: 262 IFDPAIKNRMSKILTKPDQELAEFLVGVCS-QENWEGIITKIWPNTKYLDVIVTGAMAQY 321

Query: 312 LKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEFIPLKEN--- 371
           +  L +Y G LP+    Y SSE + G N+ PM  P   ++T++PN+ YFEF+P   +   
Sbjct: 322 IPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHDGDG 381

Query: 372 -AEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLKFICRR 431
            AE    E   + L  V++G+EYE+++T  AGLYRYR+GD+++V GFHNS P+ KFI R+
Sbjct: 382 AAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRRK 441

Query: 432 NLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWEISGEA 491
           N+LL++  DK  E +LQ AVE    + A +   V+++TSY +    PGHYVI+WE+ G  
Sbjct: 442 NVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELLGRD 501

Query: 492 KG------EVLGECSNCMDRAFLDAGYMSSRKVN-AIGALELRVVRKGTFHKIMDHYLSL 551
           +       EV+ +C   M+ + L++ Y  SR  + +IG LE+RVVR GTF ++MD+ +S 
Sbjct: 502 QSNALMSEEVMAKCCLEMEES-LNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYAISR 561

Query: 552 GAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFS 573
           GA+++QYK PRCV  + T ++++L S VV+++FS
Sbjct: 562 GASINQYKVPRCV--SFTPIMELLDSRVVSAHFS 591

BLAST of Bhi01G000326 vs. Swiss-Prot
Match: sp|Q6I581|GH35_ORYSJ (Jasmonic acid-amido synthetase JAR1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=2 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 3.3e-242
Identity = 393/573 (68.59%), Postives = 480/573 (83.77%), Query Frame = 0

Query: 11  EKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFKDCVPLVTHDD 70
           E+ I EFE +T+DA RVQ++TLKKILE N SAEYLQN GL GRTD +S+K C+PL  H+D
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAESYKSCIPLCVHND 66

Query: 71  LESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTMQIYRTSFAFR 130
           +E YIQRI DGD+SP++TG+PI  +SLSSGTT G+PK IPFNDELLETT+QIYRTS+AFR
Sbjct: 67  IEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQIYRTSYAFR 126

Query: 131 NKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQSQCCSPDEVI 190
           N+E PI  GKALQF+Y SK + T GG+ A TATTN+YR  ++K  MK IQSQCCSPDEVI
Sbjct: 127 NREYPIGQGKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQSQCCSPDEVI 186

Query: 191 FGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCSNIRDGVLSSW 250
           FGPDFHQSLYCHLLCGLI+ +EV  VFSTFAHS+VH+F+TFE+VWE+LC++IRDGVLS  
Sbjct: 187 FGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTDIRDGVLSKK 246

Query: 251 VTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGIMTGSMEPYL 310
           VTAPSIR A+SK+LKPNPELAD IY+KC GLSNWYG+IP L+PNAKY+YGIMTGSMEPYL
Sbjct: 247 VTAPSIREAVSKILKPNPELADSIYKKCIGLSNWYGVIPALWPNAKYVYGIMTGSMEPYL 306

Query: 311 KKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEFIPLKENAEDQ 370
           KKLRHY G+LPL+SADYG+SEGWVG+N++P +PPE  T+ VLP +GYFEFIPL++   ++
Sbjct: 307 KKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFEFIPLEKPIGEE 366

Query: 371 QQ--------ENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLKFI 430
            +        E+ P+GLTEV++G+ YE+++TN AGLYRYRLGDVVK+  FHNSTP+L+FI
Sbjct: 367 TENSASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGDVVKIARFHNSTPELQFI 426

Query: 431 CRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWEIS 490
           CRR+L+L+INIDKNTEKDLQLAVE     L  EKLEV+DFTS+V+ S +PG YVIFWE+S
Sbjct: 427 CRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEKLEVMDFTSFVERSSDPGRYVIFWELS 486

Query: 491 GEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAAV 550
           G+A  EVL  C+N +D AF+DAGY  SRK+  IG LELR++RKGTF +I+DH+LSLG AV
Sbjct: 487 GDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILRKGTFKEILDHFLSLGGAV 546

Query: 551 SQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           SQ+KTPR VNP+N+ VLQIL  NV  SYFSTA+
Sbjct: 547 SQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAY 579

BLAST of Bhi01G000326 vs. Swiss-Prot
Match: sp|Q9SKE2|JAR1_ARATH (Jasmonic acid-amido synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 818.1 bits (2112), Expect = 5.9e-236
Identity = 390/578 (67.47%), Postives = 479/578 (82.87%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGR-TDP-QS 60
           MLEK+E FD  +VI+EF+EMT++A +VQ++TLK+IL +N SA YLQN GLNG  TDP ++
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATDPEEA 60

Query: 61  FKDCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLET 120
           FK  VPLVT  +LE YI+R+ DGD+SPILTG P+  ISLSSGT++GRPK IPF DEL+E 
Sbjct: 61  FKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMEN 120

Query: 121 TMQIYRTSFAFRNKEVPI-RNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMK 180
           T+Q++RT+FAFRN++ PI  NGKALQFI+SSK   + GG+  GTATTNVYR+  FK+ MK
Sbjct: 121 TLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMK 180

Query: 181 AIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEE 240
           +I S  CSPDEVIF PD HQ+LYCHLL G++FRD+V++VF+ FAH +VH+FRTFEQVWEE
Sbjct: 181 SITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEE 240

Query: 241 LCSNIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKY 300
           + ++I+DGVLS+ +T PS+R AMSKLL PNPELA+ I  KC  LSNWYGLIP LFPNAKY
Sbjct: 241 IVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSLSNWYGLIPALFPNAKY 300

Query: 301 IYGIMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGY 360
           +YGIMTGSMEPY+ KLRHY G LPL+S DYGSSEGW+ ANV P L PE ATF V+PN+GY
Sbjct: 301 VYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGY 360

Query: 361 FEFIPLKENAEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTP 420
           FEF+P+ E  E    E KP+GLT+VKIGEEYE+++TN AGLYRYRLGDVVKVIGF+N+TP
Sbjct: 361 FEFLPVSETGEG---EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTP 420

Query: 421 KLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVI 480
           +LKFICRRNL+L+INIDKNTE+DLQL+VE+    L+ EK+EV+DF+SY+DVS +PGHY I
Sbjct: 421 QLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEKIEVIDFSSYIDVSTDPGHYAI 480

Query: 481 FWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLS 540
           FWEISGE   +VL +C NC+DRAF+DAGY+SSRK   IGALELRVV KGTF KI +H+L 
Sbjct: 481 FWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLG 540

Query: 541 LGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           LG++  Q+K PRCV P+N  VLQILC NVV+SYFSTAF
Sbjct: 541 LGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of Bhi01G000326 vs. Swiss-Prot
Match: sp|Q53P49|GH312_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.12 PE=2 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 6.5e-174
Identity = 308/610 (50.49%), Postives = 421/610 (69.02%), Query Frame = 0

Query: 1   MLEK----MEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDP 60
           MLEK    + + + E+++  FE  T+DA  VQRETL++IL EN   EYL+ LGL G TD 
Sbjct: 1   MLEKEGKLIMSREDEEIMAWFERTTRDAADVQRETLRRILAENAGVEYLRELGLAGLTDA 60

Query: 61  QSFKDCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELL 120
            SF+  VP+VTH DL+ YIQR+ADGD+SP+LT KP+  ISLSSGTT+G+ K + FND+LL
Sbjct: 61  GSFRARVPVVTHADLDPYIQRVADGDASPVLTAKPVTAISLSSGTTQGKRKRLLFNDDLL 120

Query: 121 ETTMQIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTM 180
            ++++ +  S+AF N+  P+ +G+ LQF+Y S+   T GGL A T  TN+ RS +F ++M
Sbjct: 121 RSSIRFFHASYAFTNRAFPVEDGRVLQFMYGSRHETTKGGLTATTVMTNLLRSEEFTASM 180

Query: 181 KA---IQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQ 240
            A    +   CSP EV+F PDF +SLYCHLLCGL+   EV  V ++FAHSIV + +  E+
Sbjct: 181 AARSRPRLPSCSPSEVVFSPDFDESLYCHLLCGLLLAGEVRAVSASFAHSIVVALQALER 240

Query: 241 VWEELCSNIRDGVLS-SWVTAPSIRGAMSKLL-KPNPELADLIYRKCAGLSNWYGLIPEL 300
           VW ELC++IR G  S + VT P++R A++ +L  PNP LAD + R+CA L +W G+IP L
Sbjct: 241 VWRELCADIRRGAASPARVTTPAVRRAVAPILAAPNPALADALERRCAALGDWSGVIPAL 300

Query: 301 FPNAKYIYGIMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTV 360
           +PNA+Y+   MTGSME Y+KKLRHY G +PL+S +Y SSEG +G N     PPE   FTV
Sbjct: 301 WPNARYVQATMTGSMEHYVKKLRHYAGGVPLVSGNYASSEGVIGINAEQHAPPESVVFTV 360

Query: 361 LPNIGYFEFIPLKENAEDQQQEN-----------------KPIGLTEVKIGEEYEIIVTN 420
           LP+  YFEFIPLK    D   ++                  P+GLT+V +GE YE+++T 
Sbjct: 361 LPDAAYFEFIPLKPPCTDAAADDDNPAAAGSSCYVDADDANPVGLTDVVVGEHYEVVMTT 420

Query: 421 VAGLYRYRLGDVVKVIGFHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAA 480
             GLYRYRLGDVVKV GFH++TPKL+F+CRR+L+L+IN+DKN+E DLQLAV++   +LA 
Sbjct: 421 FTGLYRYRLGDVVKVAGFHHATPKLRFVCRRSLILSINVDKNSEHDLQLAVDSAAKILAG 480

Query: 481 E-----KLEVVDFTSYVDVSREPGHYVIFWEISG---EAKGEVLGECSNCMDRAF-LDAG 540
           +     +LE+ D+TS+ D S +PGHYV+FWE++G        VL  C + MDRAF  DAG
Sbjct: 481 DGENHKQLEIADYTSHADTSSDPGHYVVFWELNGGXXXXXXXVLQRCCDEMDRAFGADAG 540

Query: 541 YMSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSN 576
           Y  SRK  AIGALELRV+R+G F +++ HY++ G++  Q+K PRCV P+N  VL++L  N
Sbjct: 541 YAQSRKTCAIGALELRVLRRGAFQEVLRHYVAGGSSAGQFKMPRCVAPSNAGVLRVLKDN 600

BLAST of Bhi01G000326 vs. Swiss-Prot
Match: sp|Q9ZNS2|GH310_ARATH (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.8e-163
Identity = 302/593 (50.93%), Postives = 407/593 (68.63%), Query Frame = 0

Query: 2   LEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQN-LG------LNGRT 61
           +E +EA   + VI  FE ++++A +VQ ETL++ILE N   EYL+  LG      ++  T
Sbjct: 1   METVEA-GHDDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKMDDYT 60

Query: 62  DPQSFKDCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDE 121
               F   VP+V+H DL+ YIQRIADG++SP+LT +PI  +SLSSGTT+GR K +PF   
Sbjct: 61  LETLFTSLVPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRH 120

Query: 122 LLETTMQIYRTSFAFRNKEVPIR-NGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFK 181
             +TT+QI+R S A+R++  PIR  G+ L+FIY+ K  KT GGL  GTATT+ Y S +FK
Sbjct: 121 SAQTTLQIFRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFK 180

Query: 182 STMKAIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQ 241
           +  +  +S  CSP EVI G DF Q  YCHLL GL +  +VEFV S F+++IV +F  FE+
Sbjct: 181 TKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEE 240

Query: 242 VWEELCSNIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLS---NWYGLIPE 301
           +W E+C++I++G LSS +T P +R A+  L++PNP LA  I   C  L     W+GLI +
Sbjct: 241 IWREICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISK 300

Query: 302 LFPNAKYIYGIMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFT 361
           L+PNAK+I  IMTGSM PYL KLRHY G LPL+SADYGS+E W+G NV+P LPPE  +F 
Sbjct: 301 LWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFA 360

Query: 362 VLPNIGYFEFIPL--KENAED-----QQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRL 421
           V+P   YFEFIPL  ++N  D        E+KP+ L++VK+G+EYE+++T   GLYRYRL
Sbjct: 361 VIPTFSYFEFIPLYRRQNQSDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRL 420

Query: 422 GDVVKVIGFHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLA-AEKLEVVDF 481
           GDVV+V  FH  TPKL FI RR L+LTINIDKNTEKDLQ  V+    +L+ + + EVVDF
Sbjct: 421 GDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDF 480

Query: 482 TSYVDVSREPGHYVIFWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRV 541
           TS+ DV   PGHYVI+WEI GEA  + L EC   MD AF+D GY+ SR++N+IG LELRV
Sbjct: 481 TSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRV 540

Query: 542 VRKGTFHKIMDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           V +GTF K+ +  +     ++Q+KTPRC   TN+ +L IL  + +  + S+A+
Sbjct: 541 VERGTFGKVAERCVGKCGGLNQFKTPRCT--TNSVMLDILNDSTIKRFRSSAY 590

BLAST of Bhi01G000326 vs. Swiss-Prot
Match: sp|Q5NAZ7|GH33_ORYSJ (Jasmonic acid-amido synthetase JAR2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.3 PE=2 SV=2)

HSP 1 Score: 531.9 bits (1369), Expect = 8.5e-150
Identity = 269/456 (58.99%), Postives = 342/456 (75.00%), Query Frame = 0

Query: 4   KMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFKDCV 63
           +++   GE VIEEFE +T+DA  VQRETL++IL ENG  EYL+ LGL G TDP +F+  V
Sbjct: 11  RVDGVSGEAVIEEFERVTRDAANVQRETLRRILAENGGVEYLRGLGLAGATDPATFRARV 70

Query: 64  PLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTMQIY 123
           PL TH DLE YI RIADGD+SP+LT KP  +ISLSSGTT+G+ K + FN+EL+++TMQIY
Sbjct: 71  PLATHADLEPYIDRIADGDASPVLTAKPATSISLSSGTTQGKRKYLLFNEELVKSTMQIY 130

Query: 124 RTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQSQC 183
           R S+AFRN+E P+ NGKALQFIYSS+  +T GGL A TATTNVYRS +FK+TM+ IQSQC
Sbjct: 131 RISYAFRNREFPVENGKALQFIYSSRETRTKGGLTATTATTNVYRSEEFKATMRDIQSQC 190

Query: 184 CSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCSNIR 243
           CSPDEVIFGPDF QSLYCHLL GL+   +V+ V +TFAHS+V +F+TFE+ WE+LC++IR
Sbjct: 191 CSPDEVIFGPDFAQSLYCHLLAGLLAAGDVQIVSATFAHSVVLAFQTFERAWEDLCADIR 250

Query: 244 DGVLS-SWVTAPSIRGAMSKLL-KPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYGI 303
            G +S S VT+P++R AM+ LL  PNP LAD + RKCA LSNWYG+IP L+PNA+Y+YGI
Sbjct: 251 RGEVSPSRVTSPAVRRAMAALLAAPNPGLADEVARKCAALSNWYGVIPALWPNARYVYGI 310

Query: 304 MTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEFI 363
           MTGSME Y+KKLRHY G LPL++A+YG+SEGWVGANV P  PPE ATFTVLP+I YFEFI
Sbjct: 311 MTGSMEHYVKKLRHYAGGLPLVAAEYGASEGWVGANVEPGTPPERATFTVLPDIAYFEFI 370

Query: 364 PLKENAED-QQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLK 423
           PLK  A D    E +P+GLTEV  GE YE+++T  AG  R     +  V  ++  + K  
Sbjct: 371 PLKPVAGDGGYAEAEPVGLTEVAAGELYEVVMTTFAGNTRSSSSCMTLVAYYYLQSKKWM 430

Query: 424 FICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEK 457
            ICR      I++ + +   +     A G+++ A+K
Sbjct: 431 NICR----FCISVHETSRNLVTCTFAATGHIMFAQK 462

BLAST of Bhi01G000326 vs. TrEMBL
Match: tr|A0A1S4DTP6|A0A1S4DTP6_CUCME (jasmonic acid-amido synthetase JAR1 OS=Cucumis melo OX=3656 GN=LOC103483208 PE=4 SV=1)

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 545/575 (94.78%), Postives = 558/575 (97.04%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60
           MLEKMEAFDGEKVIE+FEEMT+DAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120
           DCVPLV+HDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVSHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQ 180
           QIYRTSFAFRNKEVP+  GKALQFIYSSK  KT GGLAAGTATTNVYRSA+FKSTM+AIQ
Sbjct: 121 QIYRTSFAFRNKEVPLGKGKALQFIYSSKQFKTNGGLAAGTATTNVYRSAQFKSTMRAIQ 180

Query: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240
           SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS
Sbjct: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240

Query: 241 NIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVTAPSIR AMSKLLKPNPELADLIYRKC GLSNWYGLIPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTAPSIRAAMSKLLKPNPELADLIYRKCEGLSNWYGLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEF 360
           IMTGSMEPYLKKLRHY GHLPLMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFAVLPNIGYFEF 360

Query: 361 IPLKENAEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLK 420
           IPLKENA+ Q Q NKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGD VKV+GFHNSTP+LK
Sbjct: 361 IPLKENAQGQHQRNKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDAVKVMGFHNSTPELK 420

Query: 421 FICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480
           FICRRNLLL+INIDK TEKDLQLAVEA GNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE
Sbjct: 421 FICRRNLLLSINIDKITEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480

Query: 481 ISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGA 540
           ISGEAKGEVLGECSNC+DRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDH+LSLGA
Sbjct: 481 ISGEAKGEVLGECSNCLDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHHLSLGA 540

Query: 541 AVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           AVSQYKTPRCV PTNT VLQILCSNVVNSYFSTA+
Sbjct: 541 AVSQYKTPRCVIPTNTAVLQILCSNVVNSYFSTAY 575

BLAST of Bhi01G000326 vs. TrEMBL
Match: tr|E5GCH6|E5GCH6_CUCME (Auxin-regulated protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 545/575 (94.78%), Postives = 558/575 (97.04%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60
           MLEKMEAFDGEKVIE+FEEMT+DAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120
           DCVPLV+HDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVSHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQ 180
           QIYRTSFAFRNKEVP+  GKALQFIYSSK  KT GGLAAGTATTNVYRSA+FKSTM+AIQ
Sbjct: 121 QIYRTSFAFRNKEVPLGKGKALQFIYSSKQFKTNGGLAAGTATTNVYRSAQFKSTMRAIQ 180

Query: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240
           SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS
Sbjct: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240

Query: 241 NIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVTAPSIR AMSKLLKPNPELADLIYRKC GLSNWYGLIPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTAPSIRAAMSKLLKPNPELADLIYRKCEGLSNWYGLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEF 360
           IMTGSMEPYLKKLRHY GHLPLMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFAVLPNIGYFEF 360

Query: 361 IPLKENAEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLK 420
           IPLKENA+ Q Q NKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGD VKV+GFHNSTP+LK
Sbjct: 361 IPLKENAQGQHQRNKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDAVKVMGFHNSTPELK 420

Query: 421 FICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480
           FICRRNLLL+INIDK TEKDLQLAVEA GNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE
Sbjct: 421 FICRRNLLLSINIDKITEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480

Query: 481 ISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGA 540
           ISGEAKGEVLGECSNC+DRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDH+LSLGA
Sbjct: 481 ISGEAKGEVLGECSNCLDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHHLSLGA 540

Query: 541 AVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           AVSQYKTPRCV PTNT VLQILCSNVVNSYFSTA+
Sbjct: 541 AVSQYKTPRCVIPTNTAVLQILCSNVVNSYFSTAY 575

BLAST of Bhi01G000326 vs. TrEMBL
Match: tr|A0A0A0L8M9|A0A0A0L8M9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119760 PE=4 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 531/575 (92.35%), Postives = 550/575 (95.65%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60
           MLEKMEAFDGEKVIE+FEEMT+DAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120
           DCVPLV HDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVNHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQ 180
           QIYRTSFAFRNKEVP+ NGKALQFIYSSK +KT GGLAAGTATTNVYRSA+FKSTM+AIQ
Sbjct: 121 QIYRTSFAFRNKEVPLGNGKALQFIYSSKQIKTNGGLAAGTATTNVYRSAQFKSTMQAIQ 180

Query: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240
           SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVE VFSTFAHS+VHSFRTFE+VWEELCS
Sbjct: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVESVFSTFAHSLVHSFRTFEEVWEELCS 240

Query: 241 NIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVT PSIR AMSKLLKPNPELADLIY+KC GLSNWYG+IPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTVPSIRAAMSKLLKPNPELADLIYKKCEGLSNWYGVIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEF 360
           IMTGSMEPYLKKLRHY GHLPLMSADYGSSEGWVGANV PMLPPEM TF VLPN+GYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVKPMLPPEMVTFAVLPNVGYFEF 360

Query: 361 IPLKENAEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLK 420
           IPLKE+A   Q  NKPIGLTEVKIGE YEIIVTNVAGLYRYRLGD VKV+GFHNSTP+LK
Sbjct: 361 IPLKESA---QGLNKPIGLTEVKIGEVYEIIVTNVAGLYRYRLGDAVKVMGFHNSTPELK 420

Query: 421 FICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480
           FICRRNLLL+INIDKNTEKDLQLAVEA GNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE
Sbjct: 421 FICRRNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480

Query: 481 ISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGA 540
           ISGEAKGEVL ECSNC+DRAFLDAGY+SSRKVN IGALELRVVRKGTFHKIMDH+LSLGA
Sbjct: 481 ISGEAKGEVLRECSNCLDRAFLDAGYVSSRKVNTIGALELRVVRKGTFHKIMDHHLSLGA 540

Query: 541 AVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           AVSQYKTPR V PTNT VLQILCSNVVNSYFSTA+
Sbjct: 541 AVSQYKTPRYVIPTNTAVLQILCSNVVNSYFSTAY 572

BLAST of Bhi01G000326 vs. TrEMBL
Match: tr|A0A2I4E0R7|A0A2I4E0R7_9ROSI (jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Juglans regia OX=51240 GN=LOC108985239 PE=4 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 6.1e-278
Identity = 469/585 (80.17%), Postives = 521/585 (89.06%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60
           MLEKME FD EKVIEEFE +T+DA +VQRETLKKILEENGSAEYLQ+LGLNGRTDP+SFK
Sbjct: 1   MLEKMEEFDPEKVIEEFEAITRDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 60

Query: 61  DCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120
            CVPLVTH +LE YI RIADGD SPILTGKPI TISLSSGTT+G+PK +PFNDEL+E+TM
Sbjct: 61  TCVPLVTHKELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 120

Query: 121 QIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQ 180
           QIYRTSFAFRN+E PI NGK LQFIYSSK  +T GGLAAGTATTNVYR+++FK TMKAIQ
Sbjct: 121 QIYRTSFAFRNREFPIGNGKGLQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 180

Query: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240
           SQ C PDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSI+H+FRTFEQVWEELC+
Sbjct: 181 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSILHAFRTFEQVWEELCT 240

Query: 241 NIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           +IR+GVLS+ V+APSIR AMSKLLKPNPELADL+Y+K  GLSNWY LIPELFPNAKYIYG
Sbjct: 241 DIREGVLSNRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEF 360
           IMTGSMEPYL+KLRHY G LPL+SADYGSSEGW+GANVNP LPPE ATF VLPNIG+FEF
Sbjct: 301 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPESATFAVLPNIGFFEF 360

Query: 361 IPLKENAEDQQQ----------ENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVI 420
           IPLK N EDQ Q          E++P+ LTEVKIGE+YEIIVTN AGLYRY+LGDVVKV+
Sbjct: 361 IPLKHNIEDQDQNQMDPSFFQIEHEPVDLTEVKIGEDYEIIVTNFAGLYRYKLGDVVKVM 420

Query: 421 GFHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSR 480
           GFHNSTP+LKF+CRRNLLLTINIDKNTEKDLQLAVE    +L  EKLEVVD++S+VD S 
Sbjct: 421 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 480

Query: 481 EPGHYVIFWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHK 540
           +PGHYVIFWEISGEA  EVL EC NC+DR+F+DAGY+SSRKVNAIGALELRVVR+GTF K
Sbjct: 481 DPGHYVIFWEISGEAGEEVLKECCNCLDRSFIDAGYVSSRKVNAIGALELRVVRRGTFQK 540

Query: 541 IMDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           I+DHYL LG+A+SQ+KTPRCV PTN  VLQILC NVV SYFSTAF
Sbjct: 541 ILDHYLGLGSALSQFKTPRCVGPTNNLVLQILCGNVVESYFSTAF 585

BLAST of Bhi01G000326 vs. TrEMBL
Match: tr|A0A2I4E0S1|A0A2I4E0S1_9ROSI (jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Juglans regia OX=51240 GN=LOC108985239 PE=4 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 6.1e-278
Identity = 469/585 (80.17%), Postives = 521/585 (89.06%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60
           MLEKME FD EKVIEEFE +T+DA +VQRETLKKILEENGSAEYLQ+LGLNGRTDP+SFK
Sbjct: 11  MLEKMEEFDPEKVIEEFEAITRDAGKVQRETLKKILEENGSAEYLQSLGLNGRTDPESFK 70

Query: 61  DCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120
            CVPLVTH +LE YI RIADGD SPILTGKPI TISLSSGTT+G+PK +PFNDEL+E+TM
Sbjct: 71  TCVPLVTHKELEPYIHRIADGDPSPILTGKPITTISLSSGTTQGKPKFVPFNDELMESTM 130

Query: 121 QIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQ 180
           QIYRTSFAFRN+E PI NGK LQFIYSSK  +T GGLAAGTATTNVYR+++FK TMKAIQ
Sbjct: 131 QIYRTSFAFRNREFPIGNGKGLQFIYSSKQFQTKGGLAAGTATTNVYRNSQFKETMKAIQ 190

Query: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240
           SQ C PDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSI+H+FRTFEQVWEELC+
Sbjct: 191 SQSCCPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSILHAFRTFEQVWEELCT 250

Query: 241 NIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           +IR+GVLS+ V+APSIR AMSKLLKPNPELADL+Y+K  GLSNWY LIPELFPNAKYIYG
Sbjct: 251 DIREGVLSNRVSAPSIRAAMSKLLKPNPELADLVYKKITGLSNWYRLIPELFPNAKYIYG 310

Query: 301 IMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEF 360
           IMTGSMEPYL+KLRHY G LPL+SADYGSSEGW+GANVNP LPPE ATF VLPNIG+FEF
Sbjct: 311 IMTGSMEPYLRKLRHYAGELPLLSADYGSSEGWIGANVNPKLPPESATFAVLPNIGFFEF 370

Query: 361 IPLKENAEDQQQ----------ENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVI 420
           IPLK N EDQ Q          E++P+ LTEVKIGE+YEIIVTN AGLYRY+LGDVVKV+
Sbjct: 371 IPLKHNIEDQDQNQMDPSFFQIEHEPVDLTEVKIGEDYEIIVTNFAGLYRYKLGDVVKVM 430

Query: 421 GFHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSR 480
           GFHNSTP+LKF+CRRNLLLTINIDKNTEKDLQLAVE    +L  EKLEVVD++S+VD S 
Sbjct: 431 GFHNSTPELKFVCRRNLLLTINIDKNTEKDLQLAVEEASKLLGEEKLEVVDYSSHVDSST 490

Query: 481 EPGHYVIFWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHK 540
           +PGHYVIFWEISGEA  EVL EC NC+DR+F+DAGY+SSRKVNAIGALELRVVR+GTF K
Sbjct: 491 DPGHYVIFWEISGEAGEEVLKECCNCLDRSFIDAGYVSSRKVNAIGALELRVVRRGTFQK 550

Query: 541 IMDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           I+DHYL LG+A+SQ+KTPRCV PTN  VLQILC NVV SYFSTAF
Sbjct: 551 ILDHYLGLGSALSQFKTPRCVGPTNNLVLQILCGNVVESYFSTAF 595

BLAST of Bhi01G000326 vs. NCBI nr
Match: XP_008437924.1 (PREDICTED: jasmonic acid-amido synthetase JAR1 [Cucumis melo] >XP_008437925.1 PREDICTED: jasmonic acid-amido synthetase JAR1 [Cucumis melo] >XP_016899070.1 PREDICTED: jasmonic acid-amido synthetase JAR1 [Cucumis melo] >ADN34174.1 auxin-regulated protein [Cucumis melo subsp. melo])

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 545/575 (94.78%), Postives = 558/575 (97.04%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60
           MLEKMEAFDGEKVIE+FEEMT+DAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120
           DCVPLV+HDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVSHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQ 180
           QIYRTSFAFRNKEVP+  GKALQFIYSSK  KT GGLAAGTATTNVYRSA+FKSTM+AIQ
Sbjct: 121 QIYRTSFAFRNKEVPLGKGKALQFIYSSKQFKTNGGLAAGTATTNVYRSAQFKSTMRAIQ 180

Query: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240
           SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS
Sbjct: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240

Query: 241 NIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVTAPSIR AMSKLLKPNPELADLIYRKC GLSNWYGLIPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTAPSIRAAMSKLLKPNPELADLIYRKCEGLSNWYGLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEF 360
           IMTGSMEPYLKKLRHY GHLPLMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFAVLPNIGYFEF 360

Query: 361 IPLKENAEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLK 420
           IPLKENA+ Q Q NKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGD VKV+GFHNSTP+LK
Sbjct: 361 IPLKENAQGQHQRNKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDAVKVMGFHNSTPELK 420

Query: 421 FICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480
           FICRRNLLL+INIDK TEKDLQLAVEA GNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE
Sbjct: 421 FICRRNLLLSINIDKITEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480

Query: 481 ISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGA 540
           ISGEAKGEVLGECSNC+DRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDH+LSLGA
Sbjct: 481 ISGEAKGEVLGECSNCLDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHHLSLGA 540

Query: 541 AVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           AVSQYKTPRCV PTNT VLQILCSNVVNSYFSTA+
Sbjct: 541 AVSQYKTPRCVIPTNTAVLQILCSNVVNSYFSTAY 575

BLAST of Bhi01G000326 vs. NCBI nr
Match: XP_004133822.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.5 [Cucumis sativus] >XP_011650699.1 PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.5 [Cucumis sativus] >KGN56446.1 hypothetical protein Csa_3G119760 [Cucumis sativus])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 531/575 (92.35%), Postives = 550/575 (95.65%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60
           MLEKMEAFDGEKVIE+FEEMT+DAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK
Sbjct: 1   MLEKMEAFDGEKVIEQFEEMTRDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120
           DCVPLV HDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVNHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQ 180
           QIYRTSFAFRNKEVP+ NGKALQFIYSSK +KT GGLAAGTATTNVYRSA+FKSTM+AIQ
Sbjct: 121 QIYRTSFAFRNKEVPLGNGKALQFIYSSKQIKTNGGLAAGTATTNVYRSAQFKSTMQAIQ 180

Query: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240
           SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVE VFSTFAHS+VHSFRTFE+VWEELCS
Sbjct: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVESVFSTFAHSLVHSFRTFEEVWEELCS 240

Query: 241 NIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVT PSIR AMSKLLKPNPELADLIY+KC GLSNWYG+IPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTVPSIRAAMSKLLKPNPELADLIYKKCEGLSNWYGVIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEF 360
           IMTGSMEPYLKKLRHY GHLPLMSADYGSSEGWVGANV PMLPPEM TF VLPN+GYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVKPMLPPEMVTFAVLPNVGYFEF 360

Query: 361 IPLKENAEDQQQENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIGFHNSTPKLK 420
           IPLKE+A   Q  NKPIGLTEVKIGE YEIIVTNVAGLYRYRLGD VKV+GFHNSTP+LK
Sbjct: 361 IPLKESA---QGLNKPIGLTEVKIGEVYEIIVTNVAGLYRYRLGDAVKVMGFHNSTPELK 420

Query: 421 FICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480
           FICRRNLLL+INIDKNTEKDLQLAVEA GNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE
Sbjct: 421 FICRRNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVDFTSYVDVSREPGHYVIFWE 480

Query: 481 ISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKIMDHYLSLGA 540
           ISGEAKGEVL ECSNC+DRAFLDAGY+SSRKVN IGALELRVVRKGTFHKIMDH+LSLGA
Sbjct: 481 ISGEAKGEVLRECSNCLDRAFLDAGYVSSRKVNTIGALELRVVRKGTFHKIMDHHLSLGA 540

Query: 541 AVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           AVSQYKTPR V PTNT VLQILCSNVVNSYFSTA+
Sbjct: 541 AVSQYKTPRYVIPTNTAVLQILCSNVVNSYFSTAY 572

BLAST of Bhi01G000326 vs. NCBI nr
Match: XP_022147427.1 (jasmonic acid-amido synthetase JAR1-like [Momordica charantia] >XP_022147428.1 jasmonic acid-amido synthetase JAR1-like [Momordica charantia])

HSP 1 Score: 1070.8 bits (2768), Expect = 1.6e-309
Identity = 526/584 (90.07%), Postives = 556/584 (95.21%), Query Frame = 0

Query: 1   MLEKMEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSFK 60
           ML+KMEAFDGEKVIE+FEEMTKDAERVQRETL+KILEENGSAEYLQ+LGLNGRTDPQSFK
Sbjct: 1   MLDKMEAFDGEKVIEQFEEMTKDAERVQRETLEKILEENGSAEYLQSLGLNGRTDPQSFK 60

Query: 61  DCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETTM 120
           DCVPLVTH+DLE YIQRIADG +SPILTGKPIKTISLSSGTT+GRPKLIPFNDELLETTM
Sbjct: 61  DCVPLVTHNDLEPYIQRIADGATSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETTM 120

Query: 121 QIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAIQ 180
           QIYRTSFAFRNKE PIRNGKALQFIYSSK  KT GGLAAGTATTNVYRSAKFK TMKAIQ
Sbjct: 121 QIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKCTMKAIQ 180

Query: 181 SQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELCS 240
           S CCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVH+FRTFE VWEELCS
Sbjct: 181 SLCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHAFRTFELVWEELCS 240

Query: 241 NIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300
           NIRDGVLSSWVTAP+IR AMSKLL+PNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG
Sbjct: 241 NIRDGVLSSWVTAPTIRAAMSKLLRPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIYG 300

Query: 301 IMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFEF 360
           IMTGSMEPYLKKLRHY G L LMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFEF
Sbjct: 301 IMTGSMEPYLKKLRHYAGDLLLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFEF 360

Query: 361 IPLKENAEDQQQ---------ENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKVIG 420
           IPLKE+AE+Q+Q         ENKP+GLTEVK+GEEYEIIVTNVAGLYRYRLGDVVKV+G
Sbjct: 361 IPLKESAEEQKQNEDGALVAAENKPVGLTEVKVGEEYEIIVTNVAGLYRYRLGDVVKVMG 420

Query: 421 FHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVSRE 480
           FHN+TP++KF+CRRNLLL+INIDKNTEKDLQ+AVEA G++LA EKLEVVDFTS+VD+SRE
Sbjct: 421 FHNATPEVKFVCRRNLLLSINIDKNTEKDLQVAVEAAGSLLAEEKLEVVDFTSHVDMSRE 480

Query: 481 PGHYVIFWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFHKI 540
           PGHYVIFWE SGEAKGEVLGEC NC+DRAFLDAGY+SSRKVNAIGALELRVVRKGTFHKI
Sbjct: 481 PGHYVIFWETSGEAKGEVLGECCNCLDRAFLDAGYVSSRKVNAIGALELRVVRKGTFHKI 540

Query: 541 MDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           MDHYLSLGAAVSQYKTPRCV PTNTTVLQILC+NVVNSYFSTA+
Sbjct: 541 MDHYLSLGAAVSQYKTPRCVIPTNTTVLQILCANVVNSYFSTAY 584

BLAST of Bhi01G000326 vs. NCBI nr
Match: XP_023539820.1 (jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539821.1 jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539822.1 jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1064.7 bits (2752), Expect = 1.1e-307
Identity = 527/586 (89.93%), Postives = 555/586 (94.71%), Query Frame = 0

Query: 1   MLEK-MEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSF 60
           MLEK MEAFDGEKVIE+FEEMT+DAERVQ+ETLKKILEENGSAEYLQNLGLNGRTDPQSF
Sbjct: 1   MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETT 120
           KDCVPLV HDDLESYIQRIADG SSPILTGKPIKTISLSSGTT+GRPKLIPFNDELLETT
Sbjct: 61  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120

Query: 121 MQIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAI 180
           MQIYRTSFAFRNKE PIRNGKALQFIYSSK  KT GGLAAGTATTNVYRSAKFKSTMK I
Sbjct: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTMKTI 180

Query: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELC 240
           QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVH+FRTFE VWEELC
Sbjct: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWEELC 240

Query: 241 SNIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IRDGVLSS VTA SIR AMSK+LKPNP+LADLIY+KCAGLSNWYGLIPELFPNA+YIY
Sbjct: 241 GDIRDGVLSSRVTALSIRTAMSKILKPNPDLADLIYQKCAGLSNWYGLIPELFPNAQYIY 300

Query: 301 GIMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFE 360
           GIMTGSMEPYLKKLRHY GHLPLMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFE
Sbjct: 301 GIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360

Query: 361 FIPLKENAEDQQQ----------ENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKV 420
           FIPLKEN++DQ+Q          ENKP+GLTEVK+GE YEIIVTNVAGLYRYRLGDVVKV
Sbjct: 361 FIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDVVKV 420

Query: 421 IGFHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVS 480
           +GFHN+TP+LKF+CR NLLL+INIDKNTEKDLQLAVEA GNVLAAEKLEVV+FTS+VD+S
Sbjct: 421 MGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHVDLS 480

Query: 481 REPGHYVIFWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFH 540
           REPGHYVIFWEISGEAKGEVLGEC NC+DRAFLDAGYMSSRKVNAIGALELRVV KGTFH
Sbjct: 481 REPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKGTFH 540

Query: 541 KIMDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           KIM+HYLSLGAAVSQYKTPRCV+P NTTVLQILC+NVVNSYFSTAF
Sbjct: 541 KIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVNSYFSTAF 586

BLAST of Bhi01G000326 vs. NCBI nr
Match: XP_022941396.1 (jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941403.1 jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941412.1 jasmonic acid-amido synthetase JAR1 [Cucurbita moschata])

HSP 1 Score: 1062.4 bits (2746), Expect = 5.5e-307
Identity = 526/586 (89.76%), Postives = 554/586 (94.54%), Query Frame = 0

Query: 1   MLEK-MEAFDGEKVIEEFEEMTKDAERVQRETLKKILEENGSAEYLQNLGLNGRTDPQSF 60
           MLEK MEAFDGEKVIE+FEEMT+DAERVQ+ETLKKILEENGSAEYLQNLGLNGRTDPQSF
Sbjct: 1   MLEKMMEAFDGEKVIEQFEEMTRDAERVQKETLKKILEENGSAEYLQNLGLNGRTDPQSF 60

Query: 61  KDCVPLVTHDDLESYIQRIADGDSSPILTGKPIKTISLSSGTTKGRPKLIPFNDELLETT 120
           KDCVPLV HDDLESYIQRIADG SSPILTGKPIKTISLSSGTT+GRPKLIPFNDELLETT
Sbjct: 61  KDCVPLVYHDDLESYIQRIADGASSPILTGKPIKTISLSSGTTQGRPKLIPFNDELLETT 120

Query: 121 MQIYRTSFAFRNKEVPIRNGKALQFIYSSKALKTGGGLAAGTATTNVYRSAKFKSTMKAI 180
           MQIYRTSFAFRNKE PIRNGKALQFIYSSK  KT GGLAAGTATTNVYRSAKFKSTMK I
Sbjct: 121 MQIYRTSFAFRNKEFPIRNGKALQFIYSSKQFKTKGGLAAGTATTNVYRSAKFKSTMKTI 180

Query: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVEFVFSTFAHSIVHSFRTFEQVWEELC 240
           QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEV+FVFSTFAHSIVH+FRTFE VWEELC
Sbjct: 181 QSQCCSPDEVIFGPDFHQSLYCHLLCGLIFRDEVQFVFSTFAHSIVHAFRTFELVWEELC 240

Query: 241 SNIRDGVLSSWVTAPSIRGAMSKLLKPNPELADLIYRKCAGLSNWYGLIPELFPNAKYIY 300
            +IRDGVLSS VTA SIR AMSK+LKPNPELADLIY+KCAGLSNWYGLIPELFPNA+YIY
Sbjct: 241 GDIRDGVLSSRVTALSIRTAMSKILKPNPELADLIYQKCAGLSNWYGLIPELFPNAQYIY 300

Query: 301 GIMTGSMEPYLKKLRHYGGHLPLMSADYGSSEGWVGANVNPMLPPEMATFTVLPNIGYFE 360
           GIMTGSMEPYLKKLRHY GHLPLMSADYGSSEGWVGANVNPMLPPEMATF VLPNIGYFE
Sbjct: 301 GIMTGSMEPYLKKLRHYAGHLPLMSADYGSSEGWVGANVNPMLPPEMATFVVLPNIGYFE 360

Query: 361 FIPLKENAEDQQQ----------ENKPIGLTEVKIGEEYEIIVTNVAGLYRYRLGDVVKV 420
           FIPLKEN++DQ+Q          ENKP+GLTEVK+GE YEIIVTNVAGLYRYRLGD+VKV
Sbjct: 361 FIPLKENSQDQEQEGDGGAIVAKENKPVGLTEVKVGEVYEIIVTNVAGLYRYRLGDLVKV 420

Query: 421 IGFHNSTPKLKFICRRNLLLTINIDKNTEKDLQLAVEAGGNVLAAEKLEVVDFTSYVDVS 480
           +GFHN+TP+LKF+CR NLLL+INIDKNTEKDLQLAVEA GNVLAAEKLEVV+FTS+VD+S
Sbjct: 421 MGFHNATPELKFMCRSNLLLSINIDKNTEKDLQLAVEAAGNVLAAEKLEVVEFTSHVDLS 480

Query: 481 REPGHYVIFWEISGEAKGEVLGECSNCMDRAFLDAGYMSSRKVNAIGALELRVVRKGTFH 540
           REPGHYVIFWEISGEAKGEVLGEC NC+DRAFLDAGYMSSRKVNAIGALELRVV KGTFH
Sbjct: 481 REPGHYVIFWEISGEAKGEVLGECCNCLDRAFLDAGYMSSRKVNAIGALELRVVGKGTFH 540

Query: 541 KIMDHYLSLGAAVSQYKTPRCVNPTNTTVLQILCSNVVNSYFSTAF 576
           KIM+HYLSLGAAVSQYKTPRCV+P NTTVLQILC+NVV SYFSTAF
Sbjct: 541 KIMNHYLSLGAAVSQYKTPRCVSPANTTVLQILCANVVKSYFSTAF 586

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G46370.43.3e-23767.47Auxin-responsive GH3 family protein[more]
AT4G03400.19.8e-16550.93Auxin-responsive GH3 family protein[more]
AT2G23170.11.3e-12140.28Auxin-responsive GH3 family protein[more]
AT2G14960.13.9e-12141.65Auxin-responsive GH3 family protein[more]
AT4G37390.13.9e-12140.24Auxin-responsive GH3 family protein[more]
Match NameE-valueIdentityDescription
sp|Q6I581|GH35_ORYSJ3.3e-24268.59Jasmonic acid-amido synthetase JAR1 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
sp|Q9SKE2|JAR1_ARATH5.9e-23667.47Jasmonic acid-amido synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1... [more]
sp|Q53P49|GH312_ORYSJ6.5e-17450.49Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. jap... [more]
sp|Q9ZNS2|GH310_ARATH1.8e-16350.93Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
sp|Q5NAZ7|GH33_ORYSJ8.5e-15058.99Jasmonic acid-amido synthetase JAR2 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
tr|A0A1S4DTP6|A0A1S4DTP6_CUCME0.0e+0094.78jasmonic acid-amido synthetase JAR1 OS=Cucumis melo OX=3656 GN=LOC103483208 PE=4... [more]
tr|E5GCH6|E5GCH6_CUCME0.0e+0094.78Auxin-regulated protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
tr|A0A0A0L8M9|A0A0A0L8M9_CUCSA0.0e+0092.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G119760 PE=4 SV=1[more]
tr|A0A2I4E0R7|A0A2I4E0R7_9ROSI6.1e-27880.17jasmonic acid-amido synthetase JAR1-like isoform X2 OS=Juglans regia OX=51240 GN... [more]
tr|A0A2I4E0S1|A0A2I4E0S1_9ROSI6.1e-27880.17jasmonic acid-amido synthetase JAR1-like isoform X1 OS=Juglans regia OX=51240 GN... [more]
Match NameE-valueIdentityDescription
XP_008437924.10.0e+0094.78PREDICTED: jasmonic acid-amido synthetase JAR1 [Cucumis melo] >XP_008437925.1 PR... [more]
XP_004133822.10.0e+0092.35PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.5 [Cucumis sativus... [more]
XP_022147427.11.6e-30990.07jasmonic acid-amido synthetase JAR1-like [Momordica charantia] >XP_022147428.1 j... [more]
XP_023539820.11.1e-30789.93jasmonic acid-amido synthetase JAR1 [Cucurbita pepo subsp. pepo] >XP_023539821.1... [more]
XP_022941396.15.5e-30789.76jasmonic acid-amido synthetase JAR1 [Cucurbita moschata] >XP_022941403.1 jasmoni... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0009694jasmonic acid metabolic process
GO:0009611response to wounding
GO:0009864induced systemic resistance, jasmonic acid mediated signaling pathway
Vocabulary: Molecular Function
TermDefinition
GO:0080123jasmonate-amino synthetase activity
Vocabulary: INTERPRO
TermDefinition
IPR031110JAR1
IPR004993GH3
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009864 induced systemic resistance, jasmonic acid mediated signaling pathway
biological_process GO:0010119 regulation of stomatal movement
biological_process GO:0009627 systemic acquired resistance
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0010193 response to ozone
biological_process GO:0009611 response to wounding
biological_process GO:2000377 regulation of reactive oxygen species metabolic process
biological_process GO:0009585 red, far-red light phototransduction
biological_process GO:0009640 photomorphogenesis
biological_process GO:0031348 negative regulation of defense response
cellular_component GO:0005773 vacuole
cellular_component GO:0005575 cellular_component
molecular_function GO:0070566 adenylyltransferase activity
molecular_function GO:0080123 jasmonate-amino synthetase activity
molecular_function GO:0102053 (-)-jasmonoyl-isoleucine synthetase activity
molecular_function GO:0102058 jasmonoyl-leucine synthetase activity
molecular_function GO:0102057 jasmonoyl-valine synthetase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi01M000326Bhi01M000326mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 10..30
IPR004993GH3 familyPFAMPF03321GH3coord: 16..553
e-value: 1.2E-194
score: 648.0
IPR004993GH3 familyPANTHERPTHR31901FAMILY NOT NAMEDcoord: 4..575
IPR031110Jasmonic acid-amido synthetase JAR1PANTHERPTHR31901:SF13SUBFAMILY NOT NAMEDcoord: 4..575

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi01G000326Wax gourdwgowgoB053
Bhi01G000326Cucurbita moschata (Rifu)cmowgoB0440
Bhi01G000326Bottle gourd (USVL1VR-Ls)lsiwgoB442
Bhi01G000326Watermelon (Charleston Gray)wcgwgoB437
Bhi01G000326Watermelon (97103) v2wgowmbB190