Bhi03G000004 (gene) Wax gourd

NameBhi03G000004
Typegene
OrganismBenincasa hispida (Wax gourd)
Descriptionprotein NRT1/ PTR FAMILY 4.4-like
Locationchr3 : 70960 .. 73210 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGAGAAGCTGGAGGTAGTTGAAGGAAAAGTGGACTGGAAGGGAAGACTGGCTTACAAACACAAGCATGGAGGAACTAAATCTTCTTTGCTCATACTAGGTACAAAAATGTTTTGCTCATCAAGTTCTTCAAATGTAGTGCGTCAAAAGCATATTCTAACATATATGCATATATAAAAGGAGTTATAGGAAAGAAAAAGAAAGATGCCAGAGTAAACAATTTAGCTGGCTACACAAACATATTAATATTAATCATTAATCACAACATAGTTGATTGAATTAATTTGCGCATGCAGTACTGTTGTTTACCTTTTGGGTTTCTGTTGCAGTTGCATTTGGTTTCGAGAATATGGCAAACTTTGCGCTTGCAGTAAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCAGCCAATCAGTTGACGAATTACATGGGCACTGGTTATATTCTCTCTATTTTGATGGCTATTCTCGCAGACACATACTTGGGCAGAGTCAAAATTGTCATAATTTCTGGCTGCCTTGAGTTTCTGGTTCGTCTAACTTCACTAGCTTTACTTTGCCGCCCAGCCTATTACCATGCAAATATATTCTAAACTATAACTGATAATACAGGCGCTAATATTGCTCATGATACAAGCTCACTACCCGAAGCTCAAGCCATCGCCTTGCAATATGTTTGATAAACAATCCCATTGTGAGACAGTTGAAGGAAGAAGTGCTGCCCTGCTCTATATTGCTCTCTACATATTGGCAATTGGTACTGCAGGTATTAAGGCTGCATTACCGTCACATGGTGCTGATCAATTTGATGAAAAAGACCCCAAAGAGGCGATGCAAATGTCCAGCTTCTTCAACAAACTCTTGCTAGGAGTTTGTGTCGGCGGTGCTGCTAGTTTAACTCTAATTGTTTGGATTCAGGACTACAAAGGTTGGGACTGGGGTTTAGGGGTATCTTCTGCAGCCATGTTCTTTAGTATAGTCATCTTTGCTGCCGGATTGCCACTGTACCGAATGCATATTGTTTCTGGGTCTAGTACAATCCTCCAAATTCTACAAGTATGTACATACCAACAAGCAAAATCAAGAGTCTTTTGAGGCAAATATATCATTACTTATGTCCAAATGAATGTTCTTTTCAGGTATATGTTGCAGCCATCCGTAATAGAAAACTTATTCTTCCCGAAGATTCTGCATATCTTTATGAGATTGAAAGAGACAAGGAGGCTGCTGTTGAAGAAGACTTCTTGCCTCATAGAAACATTTACAGGTTTTATACATAAAACCTTACATAAATTTTCTGCTTTCGCAGTCCAATAATAGTATTAAAATATGTATACTTATACAGGTTTTTAGACAAGGCGGCAATTCAACAAACACCTTCTAGGCAAGTTGAAAAACCCGAAGCTTCGAGTCCATGGAAACTATGCACAGTTACCCAAGTAGAGAACGCAAAAATAATTCTAAGCATGGTGCCGATTTTCTGTTGCACAATCATAATGACCCTTTGCTTGGCTCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGACACAAAACTCACCAACTCTTTCAACATCCCTCCGGCTTCACTCCCCATCATCCCGGTCTCCTTCATCATCCTCATAGTCCCGATCTACGACAAGATTTTCGTCCCCTTTGCTCGAAAGCTCACAGGCATCCCCACAGGAATCACCCACTTGCAACGAGTAGGTGTTGGATTGGTTCTGTCCAGCATCTCCATGGCCGTAGCGGCACTAGTGGAAGTGAAGCGCAAAGGCGTTGCGAGAGATCACAACATGCTGGACGCAACCCCTGTTTTGCAGCCATTACCAATCAGCACATTCTGGCTATCGTTTCAATTCTTCATATTCGGAATCGCAGATCTGTTTACGTATGTAGGACTTCTCGAATTCTTCTACTCCGAGGCGCCGAAAGCCTTGAAATCTGTATCTACTTGCTTCCTTTGGAGTTCGATGGCGTTGGGATACTTCTTGAGCACCATAATTGTGAAGATTGTGAACAGTGCTACGGAAGGAATTACGAGAAATGGAGGTTGGTTGATGGGAAACAACATTAATCGGAACCATTTGAACCTCTTCTACTGGACGCTTTCAATCTTGAGCTTGATCAACTTCTTTATCTACGTATTTGTTGCCAAGAAATACAAGTACAGAAACCATAAACCCACCATCTCCAATTCCATTGATGACAGTAGGACGCCATGA

mRNA sequence

ATGCAGGAGAAGCTGGAGGTAGTTGAAGGAAAAGTGGACTGGAAGGGAAGACTGGCTTACAAACACAAGCATGGAGGAACTAAATCTTCTTTGCTCATACTAGTTGCATTTGGTTTCGAGAATATGGCAAACTTTGCGCTTGCAGTAAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCAGCCAATCAGTTGACGAATTACATGGGCACTGGTTATATTCTCTCTATTTTGATGGCTATTCTCGCAGACACATACTTGGGCAGAGTCAAAATTGTCATAATTTCTGGCTGCCTTGAGTTTCTGGCGCTAATATTGCTCATGATACAAGCTCACTACCCGAAGCTCAAGCCATCGCCTTGCAATATGTTTGATAAACAATCCCATTGTGAGACAGTTGAAGGAAGAAGTGCTGCCCTGCTCTATATTGCTCTCTACATATTGGCAATTGGTACTGCAGGTATTAAGGCTGCATTACCGTCACATGGTGCTGATCAATTTGATGAAAAAGACCCCAAAGAGGCGATGCAAATGTCCAGCTTCTTCAACAAACTCTTGCTAGGAGTTTGTGTCGGCGGTGCTGCTAGTTTAACTCTAATTGTTTGGATTCAGGACTACAAAGGTTGGGACTGGGGTTTAGGGGTATCTTCTGCAGCCATGTTCTTTAGTATAGTCATCTTTGCTGCCGGATTGCCACTGTACCGAATGCATATTGTTTCTGGGTCTAGTACAATCCTCCAAATTCTACAAGTATATGTTGCAGCCATCCGTAATAGAAAACTTATTCTTCCCGAAGATTCTGCATATCTTTATGAGATTGAAAGAGACAAGGAGGCTGCTGTTGAAGAAGACTTCTTGCCTCATAGAAACATTTACAGGTTTTTAGACAAGGCGGCAATTCAACAAACACCTTCTAGGCAAGTTGAAAAACCCGAAGCTTCGAGTCCATGGAAACTATGCACAGTTACCCAAGTAGAGAACGCAAAAATAATTCTAAGCATGGTGCCGATTTTCTGTTGCACAATCATAATGACCCTTTGCTTGGCTCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGACACAAAACTCACCAACTCTTTCAACATCCCTCCGGCTTCACTCCCCATCATCCCGGTCTCCTTCATCATCCTCATAGTCCCGATCTACGACAAGATTTTCGTCCCCTTTGCTCGAAAGCTCACAGGCATCCCCACAGGAATCACCCACTTGCAACGAGTAGGTGTTGGATTGGTTCTGTCCAGCATCTCCATGGCCGTAGCGGCACTAGTGGAAGTGAAGCGCAAAGGCGTTGCGAGAGATCACAACATGCTGGACGCAACCCCTGTTTTGCAGCCATTACCAATCAGCACATTCTGGCTATCGTTTCAATTCTTCATATTCGGAATCGCAGATCTGTTTACGTATGTAGGACTTCTCGAATTCTTCTACTCCGAGGCGCCGAAAGCCTTGAAATCTGTATCTACTTGCTTCCTTTGGAGTTCGATGGCGTTGGGATACTTCTTGAGCACCATAATTGTGAAGATTGTGAACAGTGCTACGGAAGGAATTACGAGAAATGGAGGTTGGTTGATGGGAAACAACATTAATCGGAACCATTTGAACCTCTTCTACTGGACGCTTTCAATCTTGAGCTTGATCAACTTCTTTATCTACGTATTTGTTGCCAAGAAATACAAGTACAGAAACCATAAACCCACCATCTCCAATTCCATTGATGACAGTAGGACGCCATGA

Coding sequence (CDS)

ATGCAGGAGAAGCTGGAGGTAGTTGAAGGAAAAGTGGACTGGAAGGGAAGACTGGCTTACAAACACAAGCATGGAGGAACTAAATCTTCTTTGCTCATACTAGTTGCATTTGGTTTCGAGAATATGGCAAACTTTGCGCTTGCAGTAAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCAGCCAATCAGTTGACGAATTACATGGGCACTGGTTATATTCTCTCTATTTTGATGGCTATTCTCGCAGACACATACTTGGGCAGAGTCAAAATTGTCATAATTTCTGGCTGCCTTGAGTTTCTGGCGCTAATATTGCTCATGATACAAGCTCACTACCCGAAGCTCAAGCCATCGCCTTGCAATATGTTTGATAAACAATCCCATTGTGAGACAGTTGAAGGAAGAAGTGCTGCCCTGCTCTATATTGCTCTCTACATATTGGCAATTGGTACTGCAGGTATTAAGGCTGCATTACCGTCACATGGTGCTGATCAATTTGATGAAAAAGACCCCAAAGAGGCGATGCAAATGTCCAGCTTCTTCAACAAACTCTTGCTAGGAGTTTGTGTCGGCGGTGCTGCTAGTTTAACTCTAATTGTTTGGATTCAGGACTACAAAGGTTGGGACTGGGGTTTAGGGGTATCTTCTGCAGCCATGTTCTTTAGTATAGTCATCTTTGCTGCCGGATTGCCACTGTACCGAATGCATATTGTTTCTGGGTCTAGTACAATCCTCCAAATTCTACAAGTATATGTTGCAGCCATCCGTAATAGAAAACTTATTCTTCCCGAAGATTCTGCATATCTTTATGAGATTGAAAGAGACAAGGAGGCTGCTGTTGAAGAAGACTTCTTGCCTCATAGAAACATTTACAGGTTTTTAGACAAGGCGGCAATTCAACAAACACCTTCTAGGCAAGTTGAAAAACCCGAAGCTTCGAGTCCATGGAAACTATGCACAGTTACCCAAGTAGAGAACGCAAAAATAATTCTAAGCATGGTGCCGATTTTCTGTTGCACAATCATAATGACCCTTTGCTTGGCTCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGACACAAAACTCACCAACTCTTTCAACATCCCTCCGGCTTCACTCCCCATCATCCCGGTCTCCTTCATCATCCTCATAGTCCCGATCTACGACAAGATTTTCGTCCCCTTTGCTCGAAAGCTCACAGGCATCCCCACAGGAATCACCCACTTGCAACGAGTAGGTGTTGGATTGGTTCTGTCCAGCATCTCCATGGCCGTAGCGGCACTAGTGGAAGTGAAGCGCAAAGGCGTTGCGAGAGATCACAACATGCTGGACGCAACCCCTGTTTTGCAGCCATTACCAATCAGCACATTCTGGCTATCGTTTCAATTCTTCATATTCGGAATCGCAGATCTGTTTACGTATGTAGGACTTCTCGAATTCTTCTACTCCGAGGCGCCGAAAGCCTTGAAATCTGTATCTACTTGCTTCCTTTGGAGTTCGATGGCGTTGGGATACTTCTTGAGCACCATAATTGTGAAGATTGTGAACAGTGCTACGGAAGGAATTACGAGAAATGGAGGTTGGTTGATGGGAAACAACATTAATCGGAACCATTTGAACCTCTTCTACTGGACGCTTTCAATCTTGAGCTTGATCAACTTCTTTATCTACGTATTTGTTGCCAAGAAATACAAGTACAGAAACCATAAACCCACCATCTCCAATTCCATTGATGACAGTAGGACGCCATGA

Protein sequence

MQEKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHIVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHKPTISNSIDDSRTP
BLAST of Bhi03G000004 vs. Swiss-Prot
Match: sp|Q8H157|PTR19_ARATH (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.3e-129
Identity = 263/599 (43.91%), Postives = 377/599 (62.94%), Query Frame = 0

Query: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMH 60
           ++E++   EG  DW+ R A K +HGG  ++  +LV    EN+A  A A NL+ Y    MH
Sbjct: 3   VEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMH 62

Query: 61  FELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPK 120
              + +AN +TN+MGT ++L++L   L+D +    +I +IS  +EFL LI+L IQA  P 
Sbjct: 63  MSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPS 122

Query: 121 LKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEA 180
           L P  C+       CE V G  AA+L++ LY++A+G  GIK +L SHGA+QFDE  PK  
Sbjct: 123 LMPPSCD----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGR 182

Query: 181 MQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYR 240
            Q S+FFN  +  +  G   ++T +VW++D KGW+WG GVS+ A+F SI+IF +G   YR
Sbjct: 183 KQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYR 242

Query: 241 MHIVSGS--STILQILQVYVAAIR-----------NRKLILPEDSAYL-----YEIERDK 300
             I  GS  +TIL++L    A+++               + P +          E + + 
Sbjct: 243 NKIPCGSPLTTILKVL--LAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGEL 302

Query: 301 EAAVEEDFLPHR----NIYRFLDKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILS 360
           E   +E+ LP R    N  + L+ AA ++   R +E          CTV QVE+ KI+L 
Sbjct: 303 EKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLE----------CTVQQVEDVKIVLK 362

Query: 361 MVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYD 420
           M+PIF CTI++  CLAQL TFS+QQ  +M+TK+  S  IPPASLPI PV FI+++ PIYD
Sbjct: 363 MLPIFACTIMLNCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYD 422

Query: 421 KIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVL 480
            + +PFARK T   TG+THLQR+GVGLVLS ++MAVAALVE+KRKGVA+D  +LD+    
Sbjct: 423 HLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSK--- 482

Query: 481 QPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLST 540
           + LP++  W++ Q+   G ADLFT  GLLE+F++EAP +++S++T   W+S+A+GY+LS+
Sbjct: 483 ETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSS 542

Query: 541 IIVKIVNSATEGITRNGGWLMGNNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRN 578
           +IV IVNS T G + N  WL G +INR  L+ FYW + +LS  NF  Y+F A +YKYR+
Sbjct: 543 VIVSIVNSIT-GSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 580

BLAST of Bhi03G000004 vs. Swiss-Prot
Match: sp|Q8VYE4|PTR12_ARATH (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.8e-123
Identity = 248/569 (43.59%), Postives = 358/569 (62.92%), Query Frame = 0

Query: 9   EGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFELADAAN 68
           EG  DW+ + A + +HGG  ++  +L     EN+A  A A NL+ Y  + MH  LA +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 69  QLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKPSPCNM 128
           ++T +M T ++L++L   LAD +     I +IS  +EFL LILL IQA  P L P PC  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS 132

Query: 129 FDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 188
                 CE V G  AA L++ LY++++G  GIK +LPSHGA+QFDE  PK   Q S+FFN
Sbjct: 133 -SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 189 KLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHIVSGS- 248
             +  +  G   ++T +VWI+D KGW+WG GVS+ ++F SI++F  G   Y+  I  GS 
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 252

Query: 249 -STILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDKAAIQ 308
            +TI ++L    A+I +       +     E++ + E       L   N    L+KA   
Sbjct: 253 LTTIFKVL--LAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL--TNSLTCLNKAIEG 312

Query: 309 QTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQQGLT 368
           +T             W  CTV QVE+ KI+L M+PIF CTI++  CLAQL T+S+ Q  T
Sbjct: 313 KT----------HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT 372

Query: 369 MDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLV 428
           M+ K+ N FN+P ASLP+ PV F++++ P YD + +PFARK+T    GITHLQR+GVGLV
Sbjct: 373 MNRKIVN-FNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLV 432

Query: 429 LSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFTYVGL 488
           LS ++MAVAALVE+KRK VAR+  +LD+    + LPI+  W++ Q+   G ADLFT  GL
Sbjct: 433 LSIVAMAVAALVELKRKQVAREAGLLDSE---ETLPITFLWIALQYLFLGSADLFTLAGL 492

Query: 489 LEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNNINRN 548
           LEFF++EAP +++S++T   W+S+ALGY+LS+++V IVN  T+   ++  WL G  +NRN
Sbjct: 493 LEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQS-PWL-GEKLNRN 552

Query: 549 HLNLFYWTLSILSLINFFIYVFVAKKYKY 576
            L+LFYW + +LS++NF  Y+F AK+YKY
Sbjct: 553 RLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Bhi03G000004 vs. Swiss-Prot
Match: sp|Q56XQ6|PTR15_ARATH (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.0e-117
Identity = 246/564 (43.62%), Postives = 351/564 (62.23%), Query Frame = 0

Query: 12  VDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFELADAANQLT 71
           VD++GR     KHGGT+++L +L    FE MA  A+  NLITY  + MHF L+ +AN +T
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 72  NYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKPSPCNMFDK 131
           N++GT ++LS+L   L+D+YLG  + +++ G +E    ILL +QAH P+L+P  CNM   
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 132 QSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFNKLL 191
             HC    G  AA LY AL ++A+G+  +K  + SHGA+QF  KD +   ++SSFFN   
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKDLR---KLSSFFNAAY 206

Query: 192 LGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHIVSGSSTIL 251
               +G   +LTL+VW+Q + G D G GVS+A M   ++   AG   YR    SG S   
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSG-SIFT 266

Query: 252 QILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDKAAIQQTPSR 311
            I QV+VAAI  RK I P +   +++   D    V    L H N +RFLDKA I +T  +
Sbjct: 267 PIAQVFVAAITKRKQICPSNPNMVHQPSTD---LVRVKPLLHSNKFRFLDKACI-KTQGK 326

Query: 312 QVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKL 371
            +E     SPW+LCT+ QV   KI+LS++PIF CTII    LAQLQTFS+QQG +M+T +
Sbjct: 327 AME-----SPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 386

Query: 372 TNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSIS 431
           T +F IPPASL  IP   +I  VP+Y+  FVP ARKLTG  +GI+ LQR+G GL L++ S
Sbjct: 387 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 446

Query: 432 MAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFY 491
           M  AALVE KR+    + N++          +S FW++ QF IFG++++FT VGL+EFFY
Sbjct: 447 MVAAALVEKKRRESFLEQNVM----------LSIFWIAPQFLIFGLSEMFTAVGLVEFFY 506

Query: 492 SEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSAT--EGITRNGGWLMGNNINRNHLN 551
            ++ ++++S  T   + S + G++LS+++V  VN  T   G     GWL  N++N++ L+
Sbjct: 507 KQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLD 566

Query: 552 LFYWTLSILSLINFFIYVFVAKKY 574
            FYW L+ LS INFF Y+F ++ Y
Sbjct: 567 HFYWLLASLSFINFFNYLFWSRWY 567

BLAST of Bhi03G000004 vs. Swiss-Prot
Match: sp|Q93VV5|PTR16_ARATH (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 7.5e-117
Identity = 240/552 (43.48%), Postives = 343/552 (62.14%), Query Frame = 0

Query: 9   EGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFELADAAN 68
           E  VDW+GR +  +KHGG +++L +L    FE M   A+  NLITY  + MHF L+ AAN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 69  QLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKPSPCNM 128
            +TN++GT +I ++L   L+D +LG    +II G +E    ILL +QAH P+LKP  CN 
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 129 FDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 188
              Q+ CE  +G  A + ++ALY++A+G+  +K  + +HGADQF +  PK++ ++SS+FN
Sbjct: 147 LIDQT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFN 206

Query: 189 KLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHIVSGSS 248
                  +G   +LTL+VW+Q + G D G GVS+AAM   I+   +G  +Y  +     S
Sbjct: 207 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSG-TMYFRNKRPRRS 266

Query: 249 TILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDKAAIQQT 308
               I  V VAAI  RKL  P D   L+         V    LPH   +RFLDKA I+  
Sbjct: 267 IFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQ 326

Query: 309 PSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMD 368
            +   E     SPW+LCTVTQVE  K ++S+VPIF  TI+    LAQLQTFS+QQG +M+
Sbjct: 327 DTNTKE-----SPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 386

Query: 369 TKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLS 428
           T+L+NSF+IPPASL  IP   +I +VP+YD   VPFARKLTG  +GI  L R+G+GL LS
Sbjct: 387 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 446

Query: 429 SISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFTYVGLLE 488
           + SM  AA++E KR    RD ++LD         +S FW++ QF IFGI+++FT VGL+E
Sbjct: 447 TFSMVSAAMLEKKR----RDSSVLDGR------ILSIFWITPQFLIFGISEMFTAVGLIE 506

Query: 489 FFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNNINRNHL 548
           FFY ++ K ++S      + S + G++ S+++V +VN  T     + GWL  N++N++ L
Sbjct: 507 FFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRL 561

Query: 549 NLFYWTLSILSL 561
           +LFYW L++LSL
Sbjct: 567 DLFYWLLAVLSL 561

BLAST of Bhi03G000004 vs. Swiss-Prot
Match: sp|Q9FM20|PTR54_ARATH (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 377.1 bits (967), Expect = 3.6e-103
Identity = 221/585 (37.78%), Postives = 335/585 (57.26%), Query Frame = 0

Query: 4   KLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFEL 63
           +L    G VDW+ R A + +HGG  ++  +LV    EN+A  A A NL+ Y ++ M F  
Sbjct: 37  RLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSP 96

Query: 64  ADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKP 123
           + AAN +T +MGT + L++L   LAD +     I ++S  +EFL L++L +QAH    +P
Sbjct: 97  SGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEP 156

Query: 124 SPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQM 183
                             S   L++ LY++A+G  GIK +LP HGA+QFDE+      Q 
Sbjct: 157 -----------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQR 216

Query: 184 SSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHI 243
           S FFN  +  +  G   ++T++VW++D KGW +G GVS+AA+  S+ +F AG  +YR+ +
Sbjct: 217 SFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKV 276

Query: 244 VSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVE-------EDFLPHRNI 303
            SG S I  + +V  AA+  +           Y+  R     V        +D +  +N 
Sbjct: 277 PSG-SPITTLFKVLTAALYAK-----------YKKRRTSRIVVTCHTRNDCDDSVTKQNC 336

Query: 304 YRFLDKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQL 363
               D   +       V + E+      CT  QV++ KI++ ++PIF  TI++  CLAQL
Sbjct: 337 DG--DDGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQL 396

Query: 364 QTFSIQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGIT 423
            TFS+QQ  TM+TKL  SF +PPA+LP+ PV F++++ P Y+ + +P ARK T   TGIT
Sbjct: 397 STFSVQQASTMNTKL-GSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGIT 456

Query: 424 HLQRVGVGLVLSSISMAVAALVE--VKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFI 483
           HLQR+G GLVLS ++MAVAALVE                      PLPI+  W++ Q+  
Sbjct: 457 HLQRIGTGLVLSIVAMAVAALVEXXXXXXXXXXXXXXXXXXXXXXPLPITFLWVAIQYVF 516

Query: 484 FGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRN 543
            G ADLFT  G++EFF++EAP  ++S++T   W+S+A+GY+ S+++V  VN  T G+  +
Sbjct: 517 LGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVT-GLNHH 576

Query: 544 GGWLMGNNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHK 580
             WL+G N+N+ HL  FYW + +LS INF  Y+F A +Y YR+++
Sbjct: 577 NPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQ 588

BLAST of Bhi03G000004 vs. TAIR10
Match: AT1G69850.1 (nitrate transporter 1:2)

HSP 1 Score: 464.9 bits (1195), Expect = 7.3e-131
Identity = 263/599 (43.91%), Postives = 377/599 (62.94%), Query Frame = 0

Query: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMH 60
           ++E++   EG  DW+ R A K +HGG  ++  +LV    EN+A  A A NL+ Y    MH
Sbjct: 3   VEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMH 62

Query: 61  FELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPK 120
              + +AN +TN+MGT ++L++L   L+D +    +I +IS  +EFL LI+L IQA  P 
Sbjct: 63  MSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPS 122

Query: 121 LKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEA 180
           L P  C+       CE V G  AA+L++ LY++A+G  GIK +L SHGA+QFDE  PK  
Sbjct: 123 LMPPSCD----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGR 182

Query: 181 MQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYR 240
            Q S+FFN  +  +  G   ++T +VW++D KGW+WG GVS+ A+F SI+IF +G   YR
Sbjct: 183 KQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYR 242

Query: 241 MHIVSGS--STILQILQVYVAAIR-----------NRKLILPEDSAYL-----YEIERDK 300
             I  GS  +TIL++L    A+++               + P +          E + + 
Sbjct: 243 NKIPCGSPLTTILKVL--LAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGEL 302

Query: 301 EAAVEEDFLPHR----NIYRFLDKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILS 360
           E   +E+ LP R    N  + L+ AA ++   R +E          CTV QVE+ KI+L 
Sbjct: 303 EKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLE----------CTVQQVEDVKIVLK 362

Query: 361 MVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYD 420
           M+PIF CTI++  CLAQL TFS+QQ  +M+TK+  S  IPPASLPI PV FI+++ PIYD
Sbjct: 363 MLPIFACTIMLNCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYD 422

Query: 421 KIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVL 480
            + +PFARK T   TG+THLQR+GVGLVLS ++MAVAALVE+KRKGVA+D  +LD+    
Sbjct: 423 HLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSK--- 482

Query: 481 QPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLST 540
           + LP++  W++ Q+   G ADLFT  GLLE+F++EAP +++S++T   W+S+A+GY+LS+
Sbjct: 483 ETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSS 542

Query: 541 IIVKIVNSATEGITRNGGWLMGNNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRN 578
           +IV IVNS T G + N  WL G +INR  L+ FYW + +LS  NF  Y+F A +YKYR+
Sbjct: 543 VIVSIVNSIT-GSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRS 580

BLAST of Bhi03G000004 vs. TAIR10
Match: AT1G27040.1 (Major facilitator superfamily protein)

HSP 1 Score: 444.5 bits (1142), Expect = 1.0e-124
Identity = 248/569 (43.59%), Postives = 358/569 (62.92%), Query Frame = 0

Query: 9   EGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFELADAAN 68
           EG  DW+ + A + +HGG  ++  +L     EN+A  A A NL+ Y  + MH  LA +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 69  QLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKPSPCNM 128
           ++T +M T ++L++L   LAD +     I +IS  +EFL LILL IQA  P L P PC  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS 132

Query: 129 FDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 188
                 CE V G  AA L++ LY++++G  GIK +LPSHGA+QFDE  PK   Q S+FFN
Sbjct: 133 -SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 189 KLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHIVSGS- 248
             +  +  G   ++T +VWI+D KGW+WG GVS+ ++F SI++F  G   Y+  I  GS 
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 252

Query: 249 -STILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDKAAIQ 308
            +TI ++L    A+I +       +     E++ + E       L   N    L+KA   
Sbjct: 253 LTTIFKVL--LAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL--TNSLTCLNKAIEG 312

Query: 309 QTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQQGLT 368
           +T             W  CTV QVE+ KI+L M+PIF CTI++  CLAQL T+S+ Q  T
Sbjct: 313 KT----------HHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT 372

Query: 369 MDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLV 428
           M+ K+ N FN+P ASLP+ PV F++++ P YD + +PFARK+T    GITHLQR+GVGLV
Sbjct: 373 MNRKIVN-FNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLV 432

Query: 429 LSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFTYVGL 488
           LS ++MAVAALVE+KRK VAR+  +LD+    + LPI+  W++ Q+   G ADLFT  GL
Sbjct: 433 LSIVAMAVAALVELKRKQVAREAGLLDSE---ETLPITFLWIALQYLFLGSADLFTLAGL 492

Query: 489 LEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNNINRN 548
           LEFF++EAP +++S++T   W+S+ALGY+LS+++V IVN  T+   ++  WL G  +NRN
Sbjct: 493 LEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQS-PWL-GEKLNRN 552

Query: 549 HLNLFYWTLSILSLINFFIYVFVAKKYKY 576
            L+LFYW + +LS++NF  Y+F AK+YKY
Sbjct: 553 RLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Bhi03G000004 vs. TAIR10
Match: AT1G33440.1 (Major facilitator superfamily protein)

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-118
Identity = 246/564 (43.62%), Postives = 351/564 (62.23%), Query Frame = 0

Query: 12  VDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFELADAANQLT 71
           VD++GR     KHGGT+++L +L    FE MA  A+  NLITY  + MHF L+ +AN +T
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 72  NYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKPSPCNMFDK 131
           N++GT ++LS+L   L+D+YLG  + +++ G +E    ILL +QAH P+L+P  CNM   
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 132 QSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFNKLL 191
             HC    G  AA LY AL ++A+G+  +K  + SHGA+QF  KD +   ++SSFFN   
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKDLR---KLSSFFNAAY 206

Query: 192 LGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHIVSGSSTIL 251
               +G   +LTL+VW+Q + G D G GVS+A M   ++   AG   YR    SG S   
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSG-SIFT 266

Query: 252 QILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDKAAIQQTPSR 311
            I QV+VAAI  RK I P +   +++   D    V    L H N +RFLDKA I +T  +
Sbjct: 267 PIAQVFVAAITKRKQICPSNPNMVHQPSTD---LVRVKPLLHSNKFRFLDKACI-KTQGK 326

Query: 312 QVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKL 371
            +E     SPW+LCT+ QV   KI+LS++PIF CTII    LAQLQTFS+QQG +M+T +
Sbjct: 327 AME-----SPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 386

Query: 372 TNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSIS 431
           T +F IPPASL  IP   +I  VP+Y+  FVP ARKLTG  +GI+ LQR+G GL L++ S
Sbjct: 387 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 446

Query: 432 MAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFY 491
           M  AALVE KR+    + N++          +S FW++ QF IFG++++FT VGL+EFFY
Sbjct: 447 MVAAALVEKKRRESFLEQNVM----------LSIFWIAPQFLIFGLSEMFTAVGLVEFFY 506

Query: 492 SEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSAT--EGITRNGGWLMGNNINRNHLN 551
            ++ ++++S  T   + S + G++LS+++V  VN  T   G     GWL  N++N++ L+
Sbjct: 507 KQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLD 566

Query: 552 LFYWTLSILSLINFFIYVFVAKKY 574
            FYW L+ LS INFF Y+F ++ Y
Sbjct: 567 HFYWLLASLSFINFFNYLFWSRWY 567

BLAST of Bhi03G000004 vs. TAIR10
Match: AT1G59740.1 (Major facilitator superfamily protein)

HSP 1 Score: 422.5 bits (1085), Expect = 4.1e-118
Identity = 240/552 (43.48%), Postives = 343/552 (62.14%), Query Frame = 0

Query: 9   EGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFELADAAN 68
           E  VDW+GR +  +KHGG +++L +L    FE M   A+  NLITY  + MHF L+ AAN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 69  QLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKPSPCNM 128
            +TN++GT +I ++L   L+D +LG    +II G +E    ILL +QAH P+LKP  CN 
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 129 FDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 188
              Q+ CE  +G  A + ++ALY++A+G+  +K  + +HGADQF +  PK++ ++SS+FN
Sbjct: 147 LIDQT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFN 206

Query: 189 KLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHIVSGSS 248
                  +G   +LTL+VW+Q + G D G GVS+AAM   I+   +G  +Y  +     S
Sbjct: 207 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSG-TMYFRNKRPRRS 266

Query: 249 TILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDKAAIQQT 308
               I  V VAAI  RKL  P D   L+         V    LPH   +RFLDKA I+  
Sbjct: 267 IFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQ 326

Query: 309 PSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMD 368
            +   E     SPW+LCTVTQVE  K ++S+VPIF  TI+    LAQLQTFS+QQG +M+
Sbjct: 327 DTNTKE-----SPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 386

Query: 369 TKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLS 428
           T+L+NSF+IPPASL  IP   +I +VP+YD   VPFARKLTG  +GI  L R+G+GL LS
Sbjct: 387 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 446

Query: 429 SISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFTYVGLLE 488
           + SM  AA++E KR    RD ++LD         +S FW++ QF IFGI+++FT VGL+E
Sbjct: 447 TFSMVSAAMLEKKR----RDSSVLDGR------ILSIFWITPQFLIFGISEMFTAVGLIE 506

Query: 489 FFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNNINRNHL 548
           FFY ++ K ++S      + S + G++ S+++V +VN  T     + GWL  N++N++ L
Sbjct: 507 FFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRL 561

Query: 549 NLFYWTLSILSL 561
           +LFYW L++LSL
Sbjct: 567 DLFYWLLAVLSL 561

BLAST of Bhi03G000004 vs. TAIR10
Match: AT5G62730.1 (Major facilitator superfamily protein)

HSP 1 Score: 377.1 bits (967), Expect = 2.0e-104
Identity = 221/585 (37.78%), Postives = 335/585 (57.26%), Query Frame = 0

Query: 4   KLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFEL 63
           +L    G VDW+ R A + +HGG  ++  +LV    EN+A  A A NL+ Y ++ M F  
Sbjct: 37  RLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSP 96

Query: 64  ADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLKP 123
           + AAN +T +MGT + L++L   LAD +     I ++S  +EFL L++L +QAH    +P
Sbjct: 97  SGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEP 156

Query: 124 SPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQM 183
                             S   L++ LY++A+G  GIK +LP HGA+QFDE+      Q 
Sbjct: 157 -----------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQR 216

Query: 184 SSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMHI 243
           S FFN  +  +  G   ++T++VW++D KGW +G GVS+AA+  S+ +F AG  +YR+ +
Sbjct: 217 SFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKV 276

Query: 244 VSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVE-------EDFLPHRNI 303
            SG S I  + +V  AA+  +           Y+  R     V        +D +  +N 
Sbjct: 277 PSG-SPITTLFKVLTAALYAK-----------YKKRRTSRIVVTCHTRNDCDDSVTKQNC 336

Query: 304 YRFLDKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQL 363
               D   +       V + E+      CT  QV++ KI++ ++PIF  TI++  CLAQL
Sbjct: 337 DG--DDGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQL 396

Query: 364 QTFSIQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGIT 423
            TFS+QQ  TM+TKL  SF +PPA+LP+ PV F++++ P Y+ + +P ARK T   TGIT
Sbjct: 397 STFSVQQASTMNTKL-GSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGIT 456

Query: 424 HLQRVGVGLVLSSISMAVAALVE--VKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFI 483
           HLQR+G GLVLS ++MAVAALVE                      PLPI+  W++ Q+  
Sbjct: 457 HLQRIGTGLVLSIVAMAVAALVEXXXXXXXXXXXXXXXXXXXXXXPLPITFLWVAIQYVF 516

Query: 484 FGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRN 543
            G ADLFT  G++EFF++EAP  ++S++T   W+S+A+GY+ S+++V  VN  T G+  +
Sbjct: 517 LGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVT-GLNHH 576

Query: 544 GGWLMGNNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHK 580
             WL+G N+N+ HL  FYW + +LS INF  Y+F A +Y YR+++
Sbjct: 577 NPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQ 588

BLAST of Bhi03G000004 vs. TrEMBL
Match: tr|A0A1S3AZE0|A0A1S3AZE0_CUCME (protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484420 PE=4 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 552/592 (93.24%), Postives = 571/592 (96.45%), Query Frame = 0

Query: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMH 60
           MQEKLEVVEGKVDWKGRLAYKHKHGGT+SSLLILVAFGFE+MANFALAVNLITYFNSVMH
Sbjct: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMH 60

Query: 61  FELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNYMGTGYILSILMAILADTY GRVK VIIS C EFLALILLMIQAHYP 
Sbjct: 61  FELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPN 120

Query: 121 LKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEA 180
           LKP PCNMFDKQSHCETVEG+SAA+LY+ALYILAIG AGIKAALPSHGADQFDEKDPKEA
Sbjct: 121 LKPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYR 240
           MQMSSFFNKLLLG+C+GGA SLTLIVWIQDYKGWDWGLGVSSAAMFFS+VIF AGLPLYR
Sbjct: 181 MQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYR 240

Query: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFL 300
           MHIVSGSSTILQILQVYVAAIRNRKLILPEDS  LYEIERDKE A E+DFLPHRNIYRFL
Sbjct: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSTQLYEIERDKEVAAEDDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFS 360
           DKAAIQ+TPSRQVE PEASS WKLCTVTQVENAKI+LSMVPIFCCTIIMTLCLAQLQTFS
Sbjct: 301 DKAAIQETPSRQVENPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSF+IPPASLPIIPVSFIILIVPIYDKI VPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIVPIYDKILVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMG 540
           FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI+VKIVN ATEGITR+GGWL+G
Sbjct: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLIG 540

Query: 541 NNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHKPTISNSIDDSRTP 593
           NNINRNHLNLFYW LSILSLINFFIY+FVAKKYKYRNHKP IS +++DSRTP
Sbjct: 541 NNINRNHLNLFYWMLSILSLINFFIYIFVAKKYKYRNHKPGISKAVNDSRTP 592

BLAST of Bhi03G000004 vs. TrEMBL
Match: tr|A0A1S4DT56|A0A1S4DT56_CUCME (protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484420 PE=4 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 550/590 (93.22%), Postives = 569/590 (96.44%), Query Frame = 0

Query: 3   EKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFE 62
           EKLEVVEGKVDWKGRLAYKHKHGGT+SSLLILVAFGFE+MANFALAVNLITYFNSVMHFE
Sbjct: 2   EKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHFE 61

Query: 63  LADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLK 122
           LADAANQLTNYMGTGYILSILMAILADTY GRVK VIIS C EFLALILLMIQAHYP LK
Sbjct: 62  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNLK 121

Query: 123 PSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQ 182
           P PCNMFDKQSHCETVEG+SAA+LY+ALYILAIG AGIKAALPSHGADQFDEKDPKEAMQ
Sbjct: 122 PPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAMQ 181

Query: 183 MSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMH 242
           MSSFFNKLLLG+C+GGA SLTLIVWIQDYKGWDWGLGVSSAAMFFS+VIF AGLPLYRMH
Sbjct: 182 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRMH 241

Query: 243 IVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDK 302
           IVSGSSTILQILQVYVAAIRNRKLILPEDS  LYEIERDKE A E+DFLPHRNIYRFLDK
Sbjct: 242 IVSGSSTILQILQVYVAAIRNRKLILPEDSTQLYEIERDKEVAAEDDFLPHRNIYRFLDK 301

Query: 303 AAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQ 362
           AAIQ+TPSRQVE PEASS WKLCTVTQVENAKI+LSMVPIFCCTIIMTLCLAQLQTFSIQ
Sbjct: 302 AAIQETPSRQVENPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQ 361

Query: 363 QGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVG 422
           QGLTMDTKLTNSF+IPPASLPIIPVSFIILIVPIYDKI VPFARKLTGIPTGITHLQRVG
Sbjct: 362 QGLTMDTKLTNSFSIPPASLPIIPVSFIILIVPIYDKILVPFARKLTGIPTGITHLQRVG 421

Query: 423 VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFT 482
           VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADLFT
Sbjct: 422 VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFT 481

Query: 483 YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNN 542
           YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI+VKIVN ATEGITR+GGWL+GNN
Sbjct: 482 YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLIGNN 541

Query: 543 INRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHKPTISNSIDDSRTP 593
           INRNHLNLFYW LSILSLINFFIY+FVAKKYKYRNHKP IS +++DSRTP
Sbjct: 542 INRNHLNLFYWMLSILSLINFFIYIFVAKKYKYRNHKPGISKAVNDSRTP 591

BLAST of Bhi03G000004 vs. TrEMBL
Match: tr|A0A0A0KHY7|A0A0A0KHY7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520380 PE=4 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 2.7e-289
Identity = 512/551 (92.92%), Postives = 532/551 (96.55%), Query Frame = 0

Query: 42  MANFALAVNLITYFNSVMHFELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIIS 101
           MA FALAVNLITYFNSVMHFELADAANQLTNYMGTGYILSILMAILADTY GRVK VIIS
Sbjct: 1   MAIFALAVNLITYFNSVMHFELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS 60

Query: 102 GCLEFLALILLMIQAHYPKLKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIK 161
           GCLEFLALILLMIQAHYPKLKP PCNMFDKQSHCETVEG+SA +LYIALYILA GTAGIK
Sbjct: 61  GCLEFLALILLMIQAHYPKLKPPPCNMFDKQSHCETVEGKSAVVLYIALYILATGTAGIK 120

Query: 162 AALPSHGADQFDEKDPKEAMQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVS 221
           AALPSHGADQFDEKDPKEAMQMSSFFNKLLLG+C+GGA SLTLIVWIQDYKGWDWGLGVS
Sbjct: 121 AALPSHGADQFDEKDPKEAMQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVS 180

Query: 222 SAAMFFSIVIFAAGLPLYRMHIVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERD 281
           +AAMFFS+VIF AG+PLYR+HIVSGSSTILQILQVYVAAIRNRKLILPEDS +LYEIERD
Sbjct: 181 AAAMFFSVVIFVAGMPLYRIHIVSGSSTILQILQVYVAAIRNRKLILPEDSTHLYEIERD 240

Query: 282 KEAAVEEDFLPHRNIYRFLDKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVP 341
           KE A+E+DFLPHRNIYRFLDKAAIQQTPSRQV  PEASS WKLCTVTQVENAKI+LSMVP
Sbjct: 241 KENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSMVP 300

Query: 342 IFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIF 401
           IFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSF+IPPASLPIIPVSFIILIVPIYDKIF
Sbjct: 301 IFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIVPIYDKIF 360

Query: 402 VPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPL 461
           VPFARK TGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPV QPL
Sbjct: 361 VPFARKFTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPL 420

Query: 462 PISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIV 521
           PISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI+V
Sbjct: 421 PISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVV 480

Query: 522 KIVNSATEGITRNGGWLMGNNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHKPT 581
           KIVN ATEGITR+GGWL+GNNINRNHLNLFYW LSILSLINFFIYVFVAKKYKYRN KP 
Sbjct: 481 KIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKYRNQKPA 540

Query: 582 ISNSIDDSRTP 593
           +SN+++DSRTP
Sbjct: 541 VSNAVNDSRTP 551

BLAST of Bhi03G000004 vs. TrEMBL
Match: tr|M5WM69|M5WM69_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa002462mg PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 2.7e-249
Identity = 434/580 (74.83%), Postives = 499/580 (86.03%), Query Frame = 0

Query: 3   EKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFE 62
           +KLE+VEGKVDWKGR A K+KHGG ++++LIL  FGFENMA FALAVNL+TYFN VMHFE
Sbjct: 81  DKLELVEGKVDWKGRSAVKYKHGGMRTAVLILATFGFENMATFALAVNLVTYFNGVMHFE 140

Query: 63  LADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLK 122
           LADAANQLTNYMGTGYILSI++AILADT+ GR K ++ SGC EF+ L LL +QAHYPKL+
Sbjct: 141 LADAANQLTNYMGTGYILSIIVAILADTFFGRFKALLTSGCFEFVGLALLTVQAHYPKLR 200

Query: 123 PSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQ 182
           P  CN+FD  + CE V G +AALL+IALY+LA G AGIKA LPSHGADQFDEKDP+EA Q
Sbjct: 201 PPLCNVFDPTAMCEKVGGGNAALLFIALYMLAAGAAGIKATLPSHGADQFDEKDPREARQ 260

Query: 183 MSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMH 242
           MSSFFN LLL VC+GGA SLTLIVWIQD +GWDWG G S+ AMF  +VIF AGLPLYR+ 
Sbjct: 261 MSSFFNLLLLAVCLGGAVSLTLIVWIQDNRGWDWGFGSSTIAMFLGVVIFFAGLPLYRIQ 320

Query: 243 IVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDK 302
            + G+S I++I+QVYVAAIRNR L LPED A LYEI +DKEAA+EE+FLPHR+I+RFLDK
Sbjct: 321 PIRGTSAIVEIIQVYVAAIRNRNLSLPEDPADLYEINKDKEAALEEEFLPHRSIFRFLDK 380

Query: 303 AAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQ 362
           AAIQ + + QV++ +  +PWKLC VTQVENAKIIL MVPIF CTIIMTLCLAQLQTFSIQ
Sbjct: 381 AAIQPSTTGQVDEQQPPNPWKLCRVTQVENAKIILGMVPIFGCTIIMTLCLAQLQTFSIQ 440

Query: 363 QGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVG 422
           QGLTMDT +T SF IPPASLPIIPV F+I+I+P+YD+IFVP A K TGIP+GITHLQR+G
Sbjct: 441 QGLTMDTSITKSFKIPPASLPIIPVLFLIIIIPVYDRIFVPIASKFTGIPSGITHLQRIG 500

Query: 423 VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFT 482
           VGL+LS +SMAVA ++EVKRK VARDHNML A PVLQPLPISTFWLSFQ+FIFGIADLFT
Sbjct: 501 VGLILSCVSMAVAGIMEVKRKDVARDHNMLLARPVLQPLPISTFWLSFQYFIFGIADLFT 560

Query: 483 YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNN 542
           YVGLLEFFYSEAP  LKS+STCFLWSSMALGYF STI+VKIVN AT+ +TR+GGWL GNN
Sbjct: 561 YVGLLEFFYSEAPMGLKSISTCFLWSSMALGYFFSTILVKIVNGATKDVTRSGGWLAGNN 620

Query: 543 INRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHKPTI 583
           IN NHLNLFYW LS +SLINF +YVFVAK+YKYR   P +
Sbjct: 621 INLNHLNLFYWLLSFMSLINFIVYVFVAKRYKYRPGNPIL 660

BLAST of Bhi03G000004 vs. TrEMBL
Match: tr|A0A251PK85|A0A251PK85_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_4G070100 PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 2.7e-249
Identity = 434/580 (74.83%), Postives = 499/580 (86.03%), Query Frame = 0

Query: 3   EKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFE 62
           +KLE+VEGKVDWKGR A K+KHGG ++++LIL  FGFENMA FALAVNL+TYFN VMHFE
Sbjct: 2   DKLELVEGKVDWKGRSAVKYKHGGMRTAVLILATFGFENMATFALAVNLVTYFNGVMHFE 61

Query: 63  LADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLK 122
           LADAANQLTNYMGTGYILSI++AILADT+ GR K ++ SGC EF+ L LL +QAHYPKL+
Sbjct: 62  LADAANQLTNYMGTGYILSIIVAILADTFFGRFKALLTSGCFEFVGLALLTVQAHYPKLR 121

Query: 123 PSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQ 182
           P  CN+FD  + CE V G +AALL+IALY+LA G AGIKA LPSHGADQFDEKDP+EA Q
Sbjct: 122 PPLCNVFDPTAMCEKVGGGNAALLFIALYMLAAGAAGIKATLPSHGADQFDEKDPREARQ 181

Query: 183 MSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMH 242
           MSSFFN LLL VC+GGA SLTLIVWIQD +GWDWG G S+ AMF  +VIF AGLPLYR+ 
Sbjct: 182 MSSFFNLLLLAVCLGGAVSLTLIVWIQDNRGWDWGFGSSTIAMFLGVVIFFAGLPLYRIQ 241

Query: 243 IVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDK 302
            + G+S I++I+QVYVAAIRNR L LPED A LYEI +DKEAA+EE+FLPHR+I+RFLDK
Sbjct: 242 PIRGTSAIVEIIQVYVAAIRNRNLSLPEDPADLYEINKDKEAALEEEFLPHRSIFRFLDK 301

Query: 303 AAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQ 362
           AAIQ + + QV++ +  +PWKLC VTQVENAKIIL MVPIF CTIIMTLCLAQLQTFSIQ
Sbjct: 302 AAIQPSTTGQVDEQQPPNPWKLCRVTQVENAKIILGMVPIFGCTIIMTLCLAQLQTFSIQ 361

Query: 363 QGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVG 422
           QGLTMDT +T SF IPPASLPIIPV F+I+I+P+YD+IFVP A K TGIP+GITHLQR+G
Sbjct: 362 QGLTMDTSITKSFKIPPASLPIIPVLFLIIIIPVYDRIFVPIASKFTGIPSGITHLQRIG 421

Query: 423 VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFT 482
           VGL+LS +SMAVA ++EVKRK VARDHNML A PVLQPLPISTFWLSFQ+FIFGIADLFT
Sbjct: 422 VGLILSCVSMAVAGIMEVKRKDVARDHNMLLARPVLQPLPISTFWLSFQYFIFGIADLFT 481

Query: 483 YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNN 542
           YVGLLEFFYSEAP  LKS+STCFLWSSMALGYF STI+VKIVN AT+ +TR+GGWL GNN
Sbjct: 482 YVGLLEFFYSEAPMGLKSISTCFLWSSMALGYFFSTILVKIVNGATKDVTRSGGWLAGNN 541

Query: 543 INRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHKPTI 583
           IN NHLNLFYW LS +SLINF +YVFVAK+YKYR   P +
Sbjct: 542 INLNHLNLFYWLLSFMSLINFIVYVFVAKRYKYRPGNPIL 581

BLAST of Bhi03G000004 vs. NCBI nr
Match: XP_008439704.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucumis melo])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 552/592 (93.24%), Postives = 571/592 (96.45%), Query Frame = 0

Query: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMH 60
           MQEKLEVVEGKVDWKGRLAYKHKHGGT+SSLLILVAFGFE+MANFALAVNLITYFNSVMH
Sbjct: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMH 60

Query: 61  FELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNYMGTGYILSILMAILADTY GRVK VIIS C EFLALILLMIQAHYP 
Sbjct: 61  FELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPN 120

Query: 121 LKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEA 180
           LKP PCNMFDKQSHCETVEG+SAA+LY+ALYILAIG AGIKAALPSHGADQFDEKDPKEA
Sbjct: 121 LKPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYR 240
           MQMSSFFNKLLLG+C+GGA SLTLIVWIQDYKGWDWGLGVSSAAMFFS+VIF AGLPLYR
Sbjct: 181 MQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYR 240

Query: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFL 300
           MHIVSGSSTILQILQVYVAAIRNRKLILPEDS  LYEIERDKE A E+DFLPHRNIYRFL
Sbjct: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSTQLYEIERDKEVAAEDDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFS 360
           DKAAIQ+TPSRQVE PEASS WKLCTVTQVENAKI+LSMVPIFCCTIIMTLCLAQLQTFS
Sbjct: 301 DKAAIQETPSRQVENPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSF+IPPASLPIIPVSFIILIVPIYDKI VPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIVPIYDKILVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMG 540
           FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI+VKIVN ATEGITR+GGWL+G
Sbjct: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLIG 540

Query: 541 NNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHKPTISNSIDDSRTP 593
           NNINRNHLNLFYW LSILSLINFFIY+FVAKKYKYRNHKP IS +++DSRTP
Sbjct: 541 NNINRNHLNLFYWMLSILSLINFFIYIFVAKKYKYRNHKPGISKAVNDSRTP 592

BLAST of Bhi03G000004 vs. NCBI nr
Match: XP_016899169.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Cucumis melo])

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 550/590 (93.22%), Postives = 569/590 (96.44%), Query Frame = 0

Query: 3   EKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMHFE 62
           EKLEVVEGKVDWKGRLAYKHKHGGT+SSLLILVAFGFE+MANFALAVNLITYFNSVMHFE
Sbjct: 2   EKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHFE 61

Query: 63  LADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPKLK 122
           LADAANQLTNYMGTGYILSILMAILADTY GRVK VIIS C EFLALILLMIQAHYP LK
Sbjct: 62  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNLK 121

Query: 123 PSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEAMQ 182
           P PCNMFDKQSHCETVEG+SAA+LY+ALYILAIG AGIKAALPSHGADQFDEKDPKEAMQ
Sbjct: 122 PPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAMQ 181

Query: 183 MSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYRMH 242
           MSSFFNKLLLG+C+GGA SLTLIVWIQDYKGWDWGLGVSSAAMFFS+VIF AGLPLYRMH
Sbjct: 182 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRMH 241

Query: 243 IVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFLDK 302
           IVSGSSTILQILQVYVAAIRNRKLILPEDS  LYEIERDKE A E+DFLPHRNIYRFLDK
Sbjct: 242 IVSGSSTILQILQVYVAAIRNRKLILPEDSTQLYEIERDKEVAAEDDFLPHRNIYRFLDK 301

Query: 303 AAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFSIQ 362
           AAIQ+TPSRQVE PEASS WKLCTVTQVENAKI+LSMVPIFCCTIIMTLCLAQLQTFSIQ
Sbjct: 302 AAIQETPSRQVENPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQ 361

Query: 363 QGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVG 422
           QGLTMDTKLTNSF+IPPASLPIIPVSFIILIVPIYDKI VPFARKLTGIPTGITHLQRVG
Sbjct: 362 QGLTMDTKLTNSFSIPPASLPIIPVSFIILIVPIYDKILVPFARKLTGIPTGITHLQRVG 421

Query: 423 VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADLFT 482
           VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADLFT
Sbjct: 422 VGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFT 481

Query: 483 YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMGNN 542
           YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI+VKIVN ATEGITR+GGWL+GNN
Sbjct: 482 YVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLIGNN 541

Query: 543 INRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHKPTISNSIDDSRTP 593
           INRNHLNLFYW LSILSLINFFIY+FVAKKYKYRNHKP IS +++DSRTP
Sbjct: 542 INRNHLNLFYWMLSILSLINFFIYIFVAKKYKYRNHKPGISKAVNDSRTP 591

BLAST of Bhi03G000004 vs. NCBI nr
Match: XP_022142057.1 (protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia])

HSP 1 Score: 1039.3 bits (2686), Expect = 5.2e-300
Identity = 532/593 (89.71%), Postives = 562/593 (94.77%), Query Frame = 0

Query: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMH 60
           MQEK EVVEGKVDWK R A+KHKHGGT+SS LILVAFGFEN+ANFALAVNLITYFNSVMH
Sbjct: 1   MQEKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMH 60

Query: 61  FELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNY+GTGYI+SILMAILADTYLGRVK VIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEA 180
           LKP PCNMFDKQSHCETVEG +AALLY+ALYILAIGTAGIKAALPSH ADQFDEKDPKEA
Sbjct: 121 LKPPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEA 180

Query: 181 MQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYR 240
           MQMSSFFN LLLGVC+GGA SLTLIVWIQDYKGWDWGLGVSSAA+FFS+VIF AG+PLYR
Sbjct: 181 MQMSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYR 240

Query: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFL 300
           MHI+SGSS I+QILQVY AAIRNR L+LPEDSA LYE  RDKEAA EEDFLPHRNIYRFL
Sbjct: 241 MHIISGSSAIVQILQVYGAAIRNRNLVLPEDSADLYETVRDKEAATEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFS 360
           DKAAIQQTPS QVE PEAS+PWKLC VTQVENAKIILSMVPIFCCTI+MTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKIILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSF+IPPASLPIIPVSFIILIVPIYD+IFVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVPIYDRIFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSS+SMAVAAL+EVKRKGVARDHNMLDATPV QPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMG 540
           FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI+VK+VNS T+GIT +GGWL+G
Sbjct: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKVVNSVTKGITSSGGWLIG 540

Query: 541 NNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNH-KPTISNSIDDSRTP 593
           NNINRNHLNLFYW LSILSLINF IY+FVAK+YKYRNH KP IS++  D+R P
Sbjct: 541 NNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKYRNHNKPAISDA--DNRKP 591

BLAST of Bhi03G000004 vs. NCBI nr
Match: XP_022926310.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata])

HSP 1 Score: 1027.7 bits (2656), Expect = 1.6e-296
Identity = 522/579 (90.16%), Postives = 550/579 (94.99%), Query Frame = 0

Query: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMH 60
           MQEK EVVEGKVDWKGR A+KHKHGGT+SSLLILVAFGFENMA FALAVNLITYFN+VMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  FELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNYMG GYILSILMA+LADT+LGR K VIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEA 180
           LKP PCNMFDKQ+HCETV G +AA LY+ALY LAIG+AGIKAALPSHGADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYR 240
           +QMSSFFN+LLLGVC GGAASLTLIVWIQDYKGWDWGLG+SSAA+FF +VIF AGLPLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFL 300
           MHIVS SS I+QILQVYVAAIRNR L+LPEDSA LYEIERDKEA+VEEDFLPHRNIYRFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFS 360
           DKAAIQQTP  QVE PE SSPWK+C VTQVENAK+ILSMVPIFCCTI+MTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKICRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSF+IPPASL IIPVSFIILIVPIYDK+FVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKLFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSSISMAVAAL+E KRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMG 540
           FTYVGLLEFFYSEAPK+LKSVSTCFLWSSMALGYFLSTI+VKIVNSAT+GITRNGGWL+G
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITRNGGWLIG 540

Query: 541 NNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHK 580
           NNINRNHLNLFYW LSILS INF IYV VAK+YKYRNHK
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYVSVAKRYKYRNHK 579

BLAST of Bhi03G000004 vs. NCBI nr
Match: XP_023003153.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima])

HSP 1 Score: 1026.5 bits (2653), Expect = 3.5e-296
Identity = 522/579 (90.16%), Postives = 549/579 (94.82%), Query Frame = 0

Query: 1   MQEKLEVVEGKVDWKGRLAYKHKHGGTKSSLLILVAFGFENMANFALAVNLITYFNSVMH 60
           MQEK EVVEGKVDWKGR A+KHKHGGT+SSLLILVAFGFENMA FALAVNLITYFN+VMH
Sbjct: 1   MQEKFEVVEGKVDWKGRPAFKHKHGGTRSSLLILVAFGFENMATFALAVNLITYFNTVMH 60

Query: 61  FELADAANQLTNYMGTGYILSILMAILADTYLGRVKIVIISGCLEFLALILLMIQAHYPK 120
           FELADAANQLTNYMG GYILSILMA+LADT+LGR K VIISGCLEFLALILLMIQAHYPK
Sbjct: 61  FELADAANQLTNYMGAGYILSILMAVLADTFLGRFKTVIISGCLEFLALILLMIQAHYPK 120

Query: 121 LKPSPCNMFDKQSHCETVEGRSAALLYIALYILAIGTAGIKAALPSHGADQFDEKDPKEA 180
           LKP PCNMFDKQ+HCETV G +AA LY+ALY LAIG+AGIKAALPSHGADQFDEKDPKEA
Sbjct: 121 LKPQPCNMFDKQAHCETVGGGNAAFLYVALYTLAIGSAGIKAALPSHGADQFDEKDPKEA 180

Query: 181 MQMSSFFNKLLLGVCVGGAASLTLIVWIQDYKGWDWGLGVSSAAMFFSIVIFAAGLPLYR 240
           +QMSSFFN+LLLGVC GGAASLTLIVWIQDYKGWDWGLG+SSAA+FF +VIF AGLPLYR
Sbjct: 181 LQMSSFFNQLLLGVCGGGAASLTLIVWIQDYKGWDWGLGISSAAIFFGVVIFTAGLPLYR 240

Query: 241 MHIVSGSSTILQILQVYVAAIRNRKLILPEDSAYLYEIERDKEAAVEEDFLPHRNIYRFL 300
           MHIVS SS I+QILQVYVAAIRNR L+LPEDSA LYEIERDKEA+VEEDFLPHRNIYRFL
Sbjct: 241 MHIVSESSAIVQILQVYVAAIRNRNLVLPEDSADLYEIERDKEASVEEDFLPHRNIYRFL 300

Query: 301 DKAAIQQTPSRQVEKPEASSPWKLCTVTQVENAKIILSMVPIFCCTIIMTLCLAQLQTFS 360
           DKAAIQQTP  QVE PE SSPWKLC VTQVENAK+ILSMVPIFCCTI+MTLCLAQLQTFS
Sbjct: 301 DKAAIQQTPCGQVETPEVSSPWKLCRVTQVENAKVILSMVPIFCCTIVMTLCLAQLQTFS 360

Query: 361 IQQGLTMDTKLTNSFNIPPASLPIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420
           IQQGLTMDTKLTNSF+IPPASL IIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR
Sbjct: 361 IQQGLTMDTKLTNSFHIPPASLVIIPVSFIILIVPIYDKIFVPFARKLTGIPTGITHLQR 420

Query: 421 VGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480
           VGVGLVLSSISMAVAAL+E KRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL
Sbjct: 421 VGVGLVLSSISMAVAALMEAKRKGVARDHNMLDATPVLQPLPISTFWLSFQFFIFGIADL 480

Query: 481 FTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIIVKIVNSATEGITRNGGWLMG 540
           FTYVGLLEFFYSEAPK+LKSVSTCFLWSSMALGYFLSTI+VKIVNSAT+GIT NGGWL+G
Sbjct: 481 FTYVGLLEFFYSEAPKSLKSVSTCFLWSSMALGYFLSTIVVKIVNSATKGITSNGGWLIG 540

Query: 541 NNINRNHLNLFYWTLSILSLINFFIYVFVAKKYKYRNHK 580
           NNINRNHLNLFYW LSILS INF IY+ VAK+YKYRNHK
Sbjct: 541 NNINRNHLNLFYWLLSILSFINFCIYISVAKRYKYRNHK 579

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q8H157|PTR19_ARATH1.3e-12943.91Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
sp|Q8VYE4|PTR12_ARATH1.8e-12343.59Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
sp|Q56XQ6|PTR15_ARATH2.0e-11743.62Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
sp|Q93VV5|PTR16_ARATH7.5e-11743.48Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
sp|Q9FM20|PTR54_ARATH3.6e-10337.78Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
AT1G69850.17.3e-13143.91nitrate transporter 1:2[more]
AT1G27040.11.0e-12443.59Major facilitator superfamily protein[more]
AT1G33440.11.1e-11843.62Major facilitator superfamily protein[more]
AT1G59740.14.1e-11843.48Major facilitator superfamily protein[more]
AT5G62730.12.0e-10437.78Major facilitator superfamily protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3AZE0|A0A1S3AZE0_CUCME0.0e+0093.24protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
tr|A0A1S4DT56|A0A1S4DT56_CUCME0.0e+0093.22protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
tr|A0A0A0KHY7|A0A0A0KHY7_CUCSA2.7e-28992.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520380 PE=4 SV=1[more]
tr|M5WM69|M5WM69_PRUPE2.7e-24974.83Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa002462mg PE=4 SV=1[more]
tr|A0A251PK85|A0A251PK85_PRUPE2.7e-24974.83Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_4G070100 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008439704.10.0e+0093.24PREDICTED: protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucumis melo][more]
XP_016899169.10.0e+0093.22PREDICTED: protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Cucumis melo][more]
XP_022142057.15.2e-30089.71protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia][more]
XP_022926310.11.6e-29690.16protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata][more]
XP_023003153.13.5e-29690.16protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima][more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: Biological Process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR020846MFS_dom
IPR000109POT_fam
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 hydrogen ion transmembrane transport
biological_process GO:0035672 oligopeptide transmembrane transport
biological_process GO:0042981 regulation of apoptotic process
biological_process GO:0006810 transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0035673 oligopeptide transmembrane transporter activity
molecular_function GO:0015333 peptide:proton symporter activity
molecular_function GO:0005215 transporter activity
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M000004Bhi03M000004mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 1..582
e-value: 1.9E-172
score: 576.0
NoneNo IPR availablePANTHERPTHR11654:SF131SUBFAMILY NOT NAMEDcoord: 4..583
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 96..529
e-value: 2.2E-85
score: 286.8
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 4..583
IPR020846Major facilitator superfamily domainCDDcd06174MFScoord: 31..238
e-value: 6.38092E-6
score: 46.1529
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 32..244
coord: 311..577