Bhi04G001402 (gene) Wax gourd

NameBhi04G001402
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionATP-dependent RNA helicase
Locationchr4 : 44393707 .. 44394843 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGAAAAAGGCATGTGATTTGATTAGAAAACAGAGGAACATAATAGTTGTGGCGACGAGATTCCTCCGCCTATTAAAAATTTTAAGGACATGAGACTCCCTGAACCAGTTTTTAAAACTGAAAGAAAAAGGAATCGTGCAACCTACTCCCATTCAAGTCCAAGGTCTACCAGTGATTTTATCTGGCAGAGATATGATTGGAATTGCATTTACGGGTTCAGGAAAGACCCTAGTTTTTGTTCTTCCTTTGATAATGATTGCATTACAAGAAGAGGTCATGATGCCCATTGTTTCAGAAGGACCTTTTGGTTTAACTATTTGTCCTTCAAGGGAGCTTGCTAGACAGACTTATGAAGTGGTGGAACAGTTTTTAATTCCCATGAAGGAGGCTGGATATCCAGAACTAAAGCCTTTACTTTGCATAGGTGGAGTGGATACGAGATCACAGATAGAGGTTGTGAAGAAGAATGTTCATATTGTTGTTGCAACCCCTGGGAGGTTGAAGGATATGTTCATCTTCTGTGAGAATAAAGCTGATGTAGACGACATTCACGAGTATTTACTCTTGAAAGGTGTTGAAGCAGTGGCAATTCATGGAGGAAAAGATCAGGAAGAGAGAGAGTATGCTATATCTTCCTTCAAGGCGAGCAAGAAAGATGTATTGGTTGCTACTGATGTTGCATCAAAGGGTTTAGATTTTCCAGACATTCAACATGTTATCAACTACGATATGCCAGCTGAAATTGAGAACTATGTCCATAGGATTGGACGGACAGGACGATGTGGTAAGACAGGAATTGCAACAACATTCATAAACAAGAATCAAAGTGAAACAACTCTCCTTGATTTAAAACACCTTCTGCAAGAAGCAAAACAGAGAATTCCACCGGTGTTGGCCGAGCTGAATGACCCTATGGAGGACGTGGAAGCGATCACCAATGCAAGTGGTGTCAAAGGTTGTGCTTATTGTGGTGGACTTGGTCACCGTATCCGTGACTGCCCCAAGTTAGAGCACCAGAAAAGCATGGCAATTGCAAGTTCAAGAAGAGATTATTTTGGATCAGGCGGTTATAGAGGAGAAATTTGAGAAAGCCCAGAACATAGTAAGGATATTTTTGTTTGATTTCCCCTTCCC

mRNA sequence

ATGCCGAAAAAGGCATGTGATTTGATTAGAAAACAGAGGAACATAATAGTTTTTTTAAAACTGAAAGAAAAAGGAATCGTGCAACCTACTCCCATTCAAGTCCAAGGTCTACCAGTGATTTTATCTGGCAGAGATATGATTGGAATTGCATTTACGGGTTCAGGAAAGACCCTAGTTTTTGTTCTTCCTTTGATAATGATTGCATTACAAGAAGAGGTCATGATGCCCATTGTTTCAGAAGGACCTTTTGGTTTAACTATTTGTCCTTCAAGGGAGCTTGCTAGACAGACTTATGAAGTGGTGGAACAGTTTTTAATTCCCATGAAGGAGGCTGGATATCCAGAACTAAAGCCTTTACTTTGCATAGGTGGAGTGGATACGAGATCACAGATAGAGGTTGTGAAGAAGAATGTTCATATTGTTGTTGCAACCCCTGGGAGGTTGAAGGATATGTTCATCTTCTGTGAGAATAAAGCTGATGTAGACGACATTCACGAGTATTTACTCTTGAAAGGTGTTGAAGCAGTGGCAATTCATGGAGGAAAAGATCAGGAAGAGAGAGAGTATGCTATATCTTCCTTCAAGGCGAGCAAGAAAGATGTATTGGTTGCTACTGATGTTGCATCAAAGGGTTTAGATTTTCCAGACATTCAACATGTTATCAACTACGATATGCCAGCTGAAATTGAGAACTATGTCCATAGGATTGGACGGACAGGACGATGTGGTAAGACAGGAATTGCAACAACATTCATAAACAAGAATCAAAGTGAAACAACTCTCCTTGATTTAAAACACCTTCTGCAAGAAGCAAAACAGAGAATTCCACCGGTGTTGGCCGAGCTGAATGACCCTATGGAGGACGTGGAAGCGATCACCAATGCAAGTGGTGTCAAAGGTTGTGCTTATTGTGGTGGACTTGGTCACCGTATCCGTGACTGCCCCAAGTTAGAGCACCAGAAAAGCATGGCAATTGCAAGTTCAAGAAGAGATTATTTTGGATCAGGCGGTTATAGAGGAGAAATTTGAGAAAGCCCAGAACATAGTAAGGATATTTTTGTTTGATTTCCCCTTCCC

Coding sequence (CDS)

ATGCCGAAAAAGGCATGTGATTTGATTAGAAAACAGAGGAACATAATAGTTTTTTTAAAACTGAAAGAAAAAGGAATCGTGCAACCTACTCCCATTCAAGTCCAAGGTCTACCAGTGATTTTATCTGGCAGAGATATGATTGGAATTGCATTTACGGGTTCAGGAAAGACCCTAGTTTTTGTTCTTCCTTTGATAATGATTGCATTACAAGAAGAGGTCATGATGCCCATTGTTTCAGAAGGACCTTTTGGTTTAACTATTTGTCCTTCAAGGGAGCTTGCTAGACAGACTTATGAAGTGGTGGAACAGTTTTTAATTCCCATGAAGGAGGCTGGATATCCAGAACTAAAGCCTTTACTTTGCATAGGTGGAGTGGATACGAGATCACAGATAGAGGTTGTGAAGAAGAATGTTCATATTGTTGTTGCAACCCCTGGGAGGTTGAAGGATATGTTCATCTTCTGTGAGAATAAAGCTGATGTAGACGACATTCACGAGTATTTACTCTTGAAAGGTGTTGAAGCAGTGGCAATTCATGGAGGAAAAGATCAGGAAGAGAGAGAGTATGCTATATCTTCCTTCAAGGCGAGCAAGAAAGATGTATTGGTTGCTACTGATGTTGCATCAAAGGGTTTAGATTTTCCAGACATTCAACATGTTATCAACTACGATATGCCAGCTGAAATTGAGAACTATGTCCATAGGATTGGACGGACAGGACGATGTGGTAAGACAGGAATTGCAACAACATTCATAAACAAGAATCAAAGTGAAACAACTCTCCTTGATTTAAAACACCTTCTGCAAGAAGCAAAACAGAGAATTCCACCGGTGTTGGCCGAGCTGAATGACCCTATGGAGGACGTGGAAGCGATCACCAATGCAAGTGGTGTCAAAGGTTGTGCTTATTGTGGTGGACTTGGTCACCGTATCCGTGACTGCCCCAAGTTAGAGCACCAGAAAAGCATGGCAATTGCAAGTTCAAGAAGAGATTATTTTGGATCAGGCGGTTATAGAGGAGAAATTTGA

Protein sequence

MPKKACDLIRKQRNIIVFLKLKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSEGPFGLTICPSRELARQTYEVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDMFIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVEAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI
BLAST of Bhi04G001402 vs. Swiss-Prot
Match: sp|Q9LU46|RH35_ARATH (DEAD-box ATP-dependent RNA helicase 35 OS=Arabidopsis thaliana OX=3702 GN=RH35 PE=2 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.4e-155
Identity = 302/472 (63.98%), Postives = 320/472 (67.80%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           M  K  DLIRKQ +II                     V   LKEKGIVQPTPIQVQGLPV
Sbjct: 120 MSSKQRDLIRKQWHIIVNGDDIPPPIKNFKDMKFPRPVLDTLKEKGIVQPTPIQVQGLPV 179

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           IL+GRDMIGIAFTGSGKTLVFVLP+IMIALQEE+MMPI + EGP GL +CPSRELARQTY
Sbjct: 180 ILAGRDMIGIAFTGSGKTLVFVLPMIMIALQEEMMMPIAAGEGPIGLIVCPSRELARQTY 239

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQF+ P+ EAGYP L+ LLCIGG+D RSQ+EVVK+ VHIVVATPGRLKDM       
Sbjct: 240 EVVEQFVAPLVEAGYPPLRSLLCIGGIDMRSQLEVVKRGVHIVVATPGRLKDMLAKKKMS 299

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 300 LDACRYLTLDEADRLVDLGFEDDIREVFDHFKSQRQTLLFSATMPTKIQIFARSALVKPV 359

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 360 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTSPPVLIFCENKADVDDIHEYLLL 419

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQE+REYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 420 KGVEAVAIHGGKDQEDREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 479

BLAST of Bhi04G001402 vs. Swiss-Prot
Match: sp|Q0E3X4|RH35A_ORYSJ (DEAD-box ATP-dependent RNA helicase 35A OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0150100 PE=2 SV=2)

HSP 1 Score: 535.8 bits (1379), Expect = 3.5e-151
Identity = 281/432 (65.05%), Postives = 304/432 (70.37%), Query Frame = 0

Query: 20  KLKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIV- 79
           KL+EKGIVQPTPIQVQGLPV+LSGRDMIGIAFTGSGKTLVFVLPLIM+ALQEE+MMPIV 
Sbjct: 196 KLREKGIVQPTPIQVQGLPVVLSGRDMIGIAFTGSGKTLVFVLPLIMVALQEEMMMPIVP 255

Query: 80  SEGPFGLTICPSRELARQTYEVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNV 139
            EGPFG+ ICPSRELA+QTY+V+EQFL+P+KEAGYPE++PLLCIGGVD R+Q++VVKK V
Sbjct: 256 GEGPFGMIICPSRELAKQTYDVIEQFLVPLKEAGYPEIRPLLCIGGVDMRAQLDVVKKGV 315

Query: 140 HIVVATPGRLKDM----------------------------------------------- 199
           HIVVATPGRLKD+                                               
Sbjct: 316 HIVVATPGRLKDLLAKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLF 375

Query: 200 ------------------------------------------------------------ 259
                                                                       
Sbjct: 376 SATMPKKIQNFAKSALVKPVIVNVGRAGAANLDVIQEVEYVKEDARIIYLLECLQKTPPP 435

Query: 260 -FIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASK 319
             +FCENKADVD IHEYLLLKGVEAVAIHGGKDQEERE AI  FK  KKDVLVATDVASK
Sbjct: 436 VLVFCENKADVDYIHEYLLLKGVEAVAIHGGKDQEERENAIEFFKNGKKDVLVATDVASK 495

Query: 320 GLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQE 343
           GLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQ+ETTLLDLKHLL+E
Sbjct: 496 GLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQTETTLLDLKHLLKE 555

BLAST of Bhi04G001402 vs. Swiss-Prot
Match: sp|Q9SZB4|RH43_ARATH (Putative DEAD-box ATP-dependent RNA helicase 43 OS=Arabidopsis thaliana OX=3702 GN=RH43 PE=3 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.1e-144
Identity = 282/472 (59.75%), Postives = 307/472 (65.04%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNIIV---------------------FLKLKEKGIVQPTPIQVQGLPV 60
           M  K  DLIRKQ +I V                        LK+KGI+ PTPIQVQGLPV
Sbjct: 71  MSTKQMDLIRKQWHITVNGEDIPPPIKNFMDMKFPSPLLRMLKDKGIMHPTPIQVQGLPV 130

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           +LSGRDMIGIAFTGSGKTLVFVLP+I++ALQEE+MMPI + EGP  L ICPSRELA+QTY
Sbjct: 131 VLSGRDMIGIAFTGSGKTLVFVLPMIILALQEEIMMPIAAGEGPIALVICPSRELAKQTY 190

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKD-------- 180
           +VVEQF+  + E GYP L+ LLCIGGVD RSQ++VVKK VHIVVATPGRLKD        
Sbjct: 191 DVVEQFVASLVEDGYPRLRSLLCIGGVDMRSQLDVVKKGVHIVVATPGRLKDILAKKKMS 250

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 251 LDACRLLTLDEADRLVDLGFEDDIRHVFDHFKSQRQTLLFSATMPAKIQIFATSALVKPV 310

Query: 241 ----------------------------------------MFIFCENKADVDDIHEYLLL 300
                                                   + IFCENKADVDDIHEYLLL
Sbjct: 311 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTTPPVLIFCENKADVDDIHEYLLL 370

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQE+R+YAIS FKA KKDVLVATDVASKGLDFPDIQHVINYDMP EIE
Sbjct: 371 KGVEAVAIHGGKDQEDRDYAISLFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPGEIE 430

BLAST of Bhi04G001402 vs. Swiss-Prot
Match: sp|Q5Z6G5|RH35B_ORYSJ (DEAD-box ATP-dependent RNA helicase 35B OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0697200 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 1.8e-131
Identity = 255/432 (59.03%), Postives = 286/432 (66.20%), Query Frame = 0

Query: 21  LKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIV-S 80
           L+ KGI +PTPIQVQGLPV LSGRDMIGIAFTGSGKTLVFVLPLIM ALQEE++MPIV  
Sbjct: 188 LRGKGIEKPTPIQVQGLPVALSGRDMIGIAFTGSGKTLVFVLPLIMAALQEEILMPIVPG 247

Query: 81  EGPFGLTICPSRELARQTYEVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVH 140
           EGPFGL +CPSRELARQT+EV+E FL P+ EAGYPE++PLLCIGGVD R+Q+EVVKK VH
Sbjct: 248 EGPFGLIVCPSRELARQTHEVIEMFLAPLMEAGYPEIRPLLCIGGVDMRTQMEVVKKGVH 307

Query: 141 IVVATPGRLKDM------------------------------------------------ 200
           IVVATPGRLKD+                                                
Sbjct: 308 IVVATPGRLKDLLSKKKMNLDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFS 367

Query: 201 ------------------------------------------------------------ 260
                                                                       
Sbjct: 368 ATMPEKIQNFAKSALVKPIIVNVGRAGAANLDVIQEVEYVKEEARIIYLLECLQKTPPPV 427

Query: 261 FIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKG 320
            +FCE+KADVD I E+LLLKGVEAVAIHGGKD EER+ A  SFKAS+KDVLVATDVASKG
Sbjct: 428 LVFCEHKADVDYIQEFLLLKGVEAVAIHGGKDDEERKDAFKSFKASEKDVLVATDVASKG 487

Query: 321 LDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEA 343
           LD PDIQHVINYDMPAEIENYVHRIGRTGR GKTG+ATTFINKNQ+ETTLLDLK LL E+
Sbjct: 488 LDIPDIQHVINYDMPAEIENYVHRIGRTGRRGKTGVATTFINKNQTETTLLDLKQLLIES 547

BLAST of Bhi04G001402 vs. Swiss-Prot
Match: sp|Q54KG1|DDX41_DICDI (Probable ATP-dependent RNA helicase ddx41 OS=Dictyostelium discoideum OX=44689 GN=ddx41 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-100
Identity = 206/437 (47.14%), Postives = 254/437 (58.12%), Query Frame = 0

Query: 21  LKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIV-S 80
           L EKGI +P+PIQVQGLPVILSGRDMIGIA+TGSGKTLVF LP+++ AL+EE  +PI+  
Sbjct: 237 LLEKGIKKPSPIQVQGLPVILSGRDMIGIAYTGSGKTLVFTLPMVLFALEEECKLPIIQG 296

Query: 81  EGPFGLTICPSRELARQTYEVVEQFLIPM-KEAGYPELKPLLCIGGVDTRSQIEVVKKNV 140
           EGPFGL +CPSRELARQTY++V  F   + K  G+P+L+ LL IGG+D R Q  + KK V
Sbjct: 297 EGPFGLILCPSRELARQTYDLVNSFTNALHKNGGHPQLRTLLAIGGIDLREQEHIFKKGV 356

Query: 141 HIVVATPGRLKDM----------------------------------------------- 200
           H+++ATPGRL D+                                               
Sbjct: 357 HMIIATPGRLLDLLNKKKINFKLCKYLGLDEADRLIDLGFEDDIRSVLDNFTNQRQTLLF 416

Query: 201 ------------------------------------------------------------ 260
                                                                       
Sbjct: 417 SATMPKKIQEFARSALVLPVEVNVGRAGAANLNVTQEVEFVKPEAKIVYLLECLQKTPPP 476

Query: 261 -FIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASK 320
             IFCENK DVDDI+EYLLLK VEAV+IHG K Q+ERE AI +F+  KKDVLVATDVASK
Sbjct: 477 VLIFCENKKDVDDIYEYLLLKQVEAVSIHGDKSQDERESAIKAFREGKKDVLVATDVASK 536

Query: 321 GLDFPDIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQE 338
           GLDFP+IQHVIN+DMP EIENY+HRIGRTGR G  G+ATTFINKN +E+ LLDLK+LL E
Sbjct: 537 GLDFPEIQHVINFDMPREIENYIHRIGRTGRRGNKGVATTFINKNNTESLLLDLKYLLIE 596

BLAST of Bhi04G001402 vs. TAIR10
Match: AT5G51280.1 (DEAD-box protein abstrakt, putative)

HSP 1 Score: 550.4 bits (1417), Expect = 7.6e-157
Identity = 302/472 (63.98%), Postives = 320/472 (67.80%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           M  K  DLIRKQ +II                     V   LKEKGIVQPTPIQVQGLPV
Sbjct: 120 MSSKQRDLIRKQWHIIVNGDDIPPPIKNFKDMKFPRPVLDTLKEKGIVQPTPIQVQGLPV 179

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           IL+GRDMIGIAFTGSGKTLVFVLP+IMIALQEE+MMPI + EGP GL +CPSRELARQTY
Sbjct: 180 ILAGRDMIGIAFTGSGKTLVFVLPMIMIALQEEMMMPIAAGEGPIGLIVCPSRELARQTY 239

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQF+ P+ EAGYP L+ LLCIGG+D RSQ+EVVK+ VHIVVATPGRLKDM       
Sbjct: 240 EVVEQFVAPLVEAGYPPLRSLLCIGGIDMRSQLEVVKRGVHIVVATPGRLKDMLAKKKMS 299

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 300 LDACRYLTLDEADRLVDLGFEDDIREVFDHFKSQRQTLLFSATMPTKIQIFARSALVKPV 359

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 360 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTSPPVLIFCENKADVDDIHEYLLL 419

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQE+REYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 420 KGVEAVAIHGGKDQEDREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 479

BLAST of Bhi04G001402 vs. TAIR10
Match: AT4G33370.1 (DEA(D/H)-box RNA helicase family protein)

HSP 1 Score: 514.2 bits (1323), Expect = 6.1e-146
Identity = 282/472 (59.75%), Postives = 307/472 (65.04%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNIIV---------------------FLKLKEKGIVQPTPIQVQGLPV 60
           M  K  DLIRKQ +I V                        LK+KGI+ PTPIQVQGLPV
Sbjct: 71  MSTKQMDLIRKQWHITVNGEDIPPPIKNFMDMKFPSPLLRMLKDKGIMHPTPIQVQGLPV 130

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           +LSGRDMIGIAFTGSGKTLVFVLP+I++ALQEE+MMPI + EGP  L ICPSRELA+QTY
Sbjct: 131 VLSGRDMIGIAFTGSGKTLVFVLPMIILALQEEIMMPIAAGEGPIALVICPSRELAKQTY 190

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKD-------- 180
           +VVEQF+  + E GYP L+ LLCIGGVD RSQ++VVKK VHIVVATPGRLKD        
Sbjct: 191 DVVEQFVASLVEDGYPRLRSLLCIGGVDMRSQLDVVKKGVHIVVATPGRLKDILAKKKMS 250

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 251 LDACRLLTLDEADRLVDLGFEDDIRHVFDHFKSQRQTLLFSATMPAKIQIFATSALVKPV 310

Query: 241 ----------------------------------------MFIFCENKADVDDIHEYLLL 300
                                                   + IFCENKADVDDIHEYLLL
Sbjct: 311 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTTPPVLIFCENKADVDDIHEYLLL 370

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQE+R+YAIS FKA KKDVLVATDVASKGLDFPDIQHVINYDMP EIE
Sbjct: 371 KGVEAVAIHGGKDQEDRDYAISLFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPGEIE 430

BLAST of Bhi04G001402 vs. TAIR10
Match: AT5G63120.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 156.4 bits (394), Expect = 3.2e-38
Identity = 113/370 (30.54%), Postives = 159/370 (42.97%), Query Frame = 0

Query: 25  GIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLP-LIMIALQEEVMMPIVSEGPF 84
           G  +PTPIQ QG P+ L GRD+IGIA TGSGKTL ++LP L+ ++ Q  +      +GP 
Sbjct: 184 GFTEPTPIQAQGWPMALKGRDLIGIAETGSGKTLAYLLPALVHVSAQPRLGQ---DDGPI 243

Query: 85  GLTICPSRELARQTYEVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVA 144
            L + P+RELA Q  E   +F +    +G   ++     GG     QI  +++ V IV+A
Sbjct: 244 VLILAPTRELAVQIQEESRKFGL---RSG---VRSTCIYGGAPKGPQIRDLRRGVEIVIA 303

Query: 145 TPGRLKDM---------------------------------------------------- 204
           TPGRL DM                                                    
Sbjct: 304 TPGRLIDMLECQHTNLKRVTYLVLDEADRMLDMGFEPQIRKIVSQIRPDRQTLLWSATWP 363

Query: 205 -----------------------------------------------------------F 264
                                                                       
Sbjct: 364 REVETLARQFLRDPYKAIIGSTDLKANQSINQVIEIVPTPEKYNRLLTLLKQLMDGSKIL 423

Query: 265 IFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGL 283
           IF E K   D +   L + G  A+AIHG K Q ER+  ++ FK+ +  ++ ATDVA++GL
Sbjct: 424 IFVETKRGCDQVTRQLRMDGWPALAIHGDKTQSERDRVLAEFKSGRSPIMTATDVAARGL 483

BLAST of Bhi04G001402 vs. TAIR10
Match: AT3G58570.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 156.0 bits (393), Expect = 4.2e-38
Identity = 106/381 (27.82%), Postives = 157/381 (41.21%), Query Frame = 0

Query: 19  LKLKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLIM-IALQEEVMMPI 78
           L ++    V+PTP+Q   +P++ +GRD++  A TGSGKT  F  P+I  I   + +  P 
Sbjct: 159 LNIQRCKYVKPTPVQRNAIPILAAGRDLMACAQTGSGKTAAFCFPIISGIMKDQHIERPR 218

Query: 79  VSEG--PFGLTICPSRELARQTYEVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVK 138
              G  P  + + P+RELA Q ++   +F      +    +K ++  GG     QI  ++
Sbjct: 219 GVRGVYPLAVILSPTRELACQIHDEARKF------SYQTGVKVVVAYGGTPVNQQIRELE 278

Query: 139 KNVHIVVATPGRLKDM-------------------------------------------- 198
           + V I+VATPGRL D+                                            
Sbjct: 279 RGVDILVATPGRLNDLLERGRVSLQMVRFLALDEADRMLDMGFEPQIRKIVQQMDMPPPG 338

Query: 199 ------------------------------------------------------------ 258
                                                                       
Sbjct: 339 VRQTMLFSATFPREIQRLASDFLSNYIFLAVGRVGSSTDLIVQRVEFVHDSDKRSHLMDL 398

Query: 259 ----------------FIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISSFK 277
                            +F E K   D +  +L + G  A  IHG + Q+ERE A+ SFK
Sbjct: 399 LHAQRENGNQGKQALTLVFVETKKGADSLENWLCINGFPATTIHGDRSQQEREVALRSFK 458

BLAST of Bhi04G001402 vs. TAIR10
Match: AT2G33730.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 153.7 bits (387), Expect = 2.1e-37
Identity = 110/387 (28.42%), Postives = 157/387 (40.57%), Query Frame = 0

Query: 21  LKEKGIVQPTPIQVQGLPVILSGRDMIGIAFTGSGKTLVFVLPLI-MIALQEEVMMPIVS 80
           ++  G  +P+PIQ+  +P+ L  RD+IGIA TGSGKT  FVLP++  I+    +     +
Sbjct: 328 VERAGYKKPSPIQMAAIPLGLQQRDVIGIAETGSGKTAAFVLPMLAYISRLPPMSEENET 387

Query: 81  EGPFGLTICPSRELARQTYEVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVH 140
           EGP+ + + P+RELA+Q  E   +F      A Y   +    +GG     Q   + +   
Sbjct: 388 EGPYAVVMAPTRELAQQIEEETVKF------AHYLGFRVTSIVGGQSIEEQGLKITQGCE 447

Query: 141 IVVATPGRL--------------------------------------------------- 200
           IV+ATPGRL                                                   
Sbjct: 448 IVIATPGRLIDCLERRYAVLNQCNYVVLDEADRMIDMGFEPQVAGVLDAMPSSNLKPENE 507

Query: 201 ------------------------------------------------------------ 260
                                                                       
Sbjct: 508 EEELDEKKIYRTTYMFSATMPPGVERLARKYLRNPVVVTIGTAGKTTDLISQHVIMMKES 567

Query: 261 ---------------KDMFIFCENKADVDDIHEYLLLKGVEAVAIHGGKDQEEREYAISS 281
                          K   +F   K + D I + L   G     +HGGK QE+RE ++  
Sbjct: 568 EKFFRLQKLLDELGEKTAIVFVNTKKNCDSIAKNLDKAGYRVTTLHGGKSQEQREISLEG 627

BLAST of Bhi04G001402 vs. TrEMBL
Match: tr|A0A1S3CDK5|A0A1S3CDK5_CUCME (DEAD-box ATP-dependent RNA helicase 35 OS=Cucumis melo OX=3656 GN=LOC103499818 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 6.2e-169
Identity = 333/472 (70.55%), Postives = 335/472 (70.97%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           MPKKACDLIRKQ +II                     V  KLKEKGIVQPTPIQVQGLPV
Sbjct: 126 MPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPV 185

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS EGPFGL ICPSRELARQTY
Sbjct: 186 ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTY 245

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQFLIPMKEAGYPEL+PLLCIGGVD RSQIEVVKK VHIVVATPGRLKDM       
Sbjct: 246 EVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMN 305

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 306 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 365

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 366 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 425

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 426 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 485

BLAST of Bhi04G001402 vs. TrEMBL
Match: tr|A0A0A0K6A2|A0A0A0K6A2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G432260 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 6.2e-169
Identity = 333/472 (70.55%), Postives = 335/472 (70.97%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           MPKKACDLIRKQ +II                     V  KLKEKGIVQPTPIQVQGLPV
Sbjct: 246 MPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPV 305

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS EGPFGL ICPSRELARQTY
Sbjct: 306 ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTY 365

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQFLIPMKEAGYPEL+PLLCIGGVD RSQIEVVKK VHIVVATPGRLKDM       
Sbjct: 366 EVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMN 425

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 426 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 485

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 486 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 545

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 546 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 605

BLAST of Bhi04G001402 vs. TrEMBL
Match: tr|A0A200Q5Y8|A0A200Q5Y8_9MAGN (Helicase OS=Macleaya cordata OX=56857 GN=BVC80_311g2 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 5.6e-162
Identity = 316/472 (66.95%), Postives = 328/472 (69.49%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           M  K CDLIRKQ +I+                     V  KLKEKGIVQPTPIQVQGLPV
Sbjct: 122 MSSKQCDLIRKQWHILVDGDEVPPPIKNFKDMRFPEPVLKKLKEKGIVQPTPIQVQGLPV 181

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIV-SEGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIM+ALQEE+MMPIV  EGPFGL ICPSRELARQTY
Sbjct: 182 ILSGRDMIGIAFTGSGKTLVFVLPLIMVALQEEMMMPIVPGEGPFGLVICPSRELARQTY 241

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVE+FLIP+KE GYPEL+PLLCIGGVD RSQ+EVVKK VHIVVATPGRLKDM       
Sbjct: 242 EVVEEFLIPLKEMGYPELRPLLCIGGVDMRSQLEVVKKGVHIVVATPGRLKDMLAKKKMN 301

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 302 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 361

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 362 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPSPVLIFCENKADVDDIHEYLLL 421

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAI+SFK  KKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 422 KGVEAVAIHGGKDQEEREYAIASFKTGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 481

BLAST of Bhi04G001402 vs. TrEMBL
Match: tr|M5WR00|M5WR00_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G092400 PE=4 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 9.5e-162
Identity = 316/472 (66.95%), Postives = 330/472 (69.92%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNIIV--------------------FLK-LKEKGIVQPTPIQVQGLPV 60
           MP K CDL+RKQ +IIV                     LK LK KGIVQPTPIQVQGLPV
Sbjct: 116 MPSKQCDLVRKQWHIIVSGEDVPPPIKNFKDMRFPEPILKMLKTKGIVQPTPIQVQGLPV 175

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIV-SEGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIM+ALQEE+MMPIV  EGPFGL ICPSRELARQTY
Sbjct: 176 ILSGRDMIGIAFTGSGKTLVFVLPLIMVALQEEIMMPIVPGEGPFGLIICPSRELARQTY 235

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVE+FLIP++EAGYPE++PLLCIGGVD RSQ+E+VKK VHIVVATPGRLKDM       
Sbjct: 236 EVVEEFLIPLREAGYPEIRPLLCIGGVDMRSQLEIVKKGVHIVVATPGRLKDMLAKKKMN 295

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 296 LNNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFAKSALVKPV 355

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 356 LVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 415

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAISSFKA KKDVLVATDVASKGLDFPDIQHVINYDMP EIE
Sbjct: 416 KGVEAVAIHGGKDQEEREYAISSFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPPEIE 475

BLAST of Bhi04G001402 vs. TrEMBL
Match: tr|A0A1U7ZC92|A0A1U7ZC92_NELNU (DEAD-box ATP-dependent RNA helicase 35 OS=Nelumbo nucifera OX=4432 GN=LOC104588602 PE=4 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 1.2e-161
Identity = 316/472 (66.95%), Postives = 328/472 (69.49%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNIIV---------------------FLKLKEKGIVQPTPIQVQGLPV 60
           +P+K CD IRKQ +I+V                       KLK KGIVQPTPIQVQGLPV
Sbjct: 122 IPQKECDSIRKQWHILVDGEDVPPPIKNFKDMRLPEPILKKLKAKGIVQPTPIQVQGLPV 181

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIV-SEGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIM ALQEEV MPIV  EGPFGL ICPSRELARQTY
Sbjct: 182 ILSGRDMIGIAFTGSGKTLVFVLPLIMAALQEEVTMPIVPGEGPFGLVICPSRELARQTY 241

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQFLIP+KE+GYPEL+PLLCIGGVD RSQ+EVVKK VHIVVATPGRLKDM       
Sbjct: 242 EVVEQFLIPLKESGYPELRPLLCIGGVDMRSQLEVVKKGVHIVVATPGRLKDMLAKKKMN 301

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 302 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 361

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 362 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 421

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAI+SFKA KKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 422 KGVEAVAIHGGKDQEEREYAIASFKAGKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 481

BLAST of Bhi04G001402 vs. NCBI nr
Match: XP_004135864.1 (PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis sativus])

HSP 1 Score: 602.4 bits (1552), Expect = 9.3e-169
Identity = 333/472 (70.55%), Postives = 335/472 (70.97%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           MPKKACDLIRKQ +II                     V  KLKEKGIVQPTPIQVQGLPV
Sbjct: 126 MPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPV 185

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS EGPFGL ICPSRELARQTY
Sbjct: 186 ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTY 245

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQFLIPMKEAGYPEL+PLLCIGGVD RSQIEVVKK VHIVVATPGRLKDM       
Sbjct: 246 EVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMN 305

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 306 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 365

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 366 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 425

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 426 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 485

BLAST of Bhi04G001402 vs. NCBI nr
Match: XP_008461142.1 (PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo] >XP_008461143.1 PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo])

HSP 1 Score: 602.4 bits (1552), Expect = 9.3e-169
Identity = 333/472 (70.55%), Postives = 335/472 (70.97%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           MPKKACDLIRKQ +II                     V  KLKEKGIVQPTPIQVQGLPV
Sbjct: 126 MPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPV 185

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS EGPFGL ICPSRELARQTY
Sbjct: 186 ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTY 245

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQFLIPMKEAGYPEL+PLLCIGGVD RSQIEVVKK VHIVVATPGRLKDM       
Sbjct: 246 EVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMN 305

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 306 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 365

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 366 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 425

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 426 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 485

BLAST of Bhi04G001402 vs. NCBI nr
Match: KGN45245.1 (hypothetical protein Csa_7G432260 [Cucumis sativus])

HSP 1 Score: 602.4 bits (1552), Expect = 9.3e-169
Identity = 333/472 (70.55%), Postives = 335/472 (70.97%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           MPKKACDLIRKQ +II                     V  KLKEKGIVQPTPIQVQGLPV
Sbjct: 246 MPKKACDLIRKQWHIIVDGDEIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPV 305

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS EGPFGL ICPSRELARQTY
Sbjct: 306 ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTY 365

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQFLIPMKEAGYPEL+PLLCIGGVD RSQIEVVKK VHIVVATPGRLKDM       
Sbjct: 366 EVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEVVKKGVHIVVATPGRLKDMLAKKKMN 425

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 426 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 485

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 486 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 545

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 546 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 605

BLAST of Bhi04G001402 vs. NCBI nr
Match: XP_022960221.1 (DEAD-box ATP-dependent RNA helicase 35-like [Cucurbita moschata] >XP_022960222.1 DEAD-box ATP-dependent RNA helicase 35-like [Cucurbita moschata])

HSP 1 Score: 600.9 bits (1548), Expect = 2.7e-168
Identity = 331/472 (70.13%), Postives = 335/472 (70.97%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           MP+KACDLIRKQ +II                     V  KLKEKGIVQPTPIQVQGLPV
Sbjct: 127 MPRKACDLIRKQWHIIVDGDNIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPV 186

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS EGPFGL ICPSRELARQTY
Sbjct: 187 ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTY 246

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQFLIPMKEAGYPEL+PLLCIGGVD RSQIE+VKK VHIVVATPGRLKDM       
Sbjct: 247 EVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMN 306

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 307 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 366

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 367 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 426

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 427 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 486

BLAST of Bhi04G001402 vs. NCBI nr
Match: XP_023004285.1 (DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023004286.1 DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023514207.1 DEAD-box ATP-dependent RNA helicase 35 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 600.9 bits (1548), Expect = 2.7e-168
Identity = 331/472 (70.13%), Postives = 335/472 (70.97%), Query Frame = 0

Query: 1   MPKKACDLIRKQRNII---------------------VFLKLKEKGIVQPTPIQVQGLPV 60
           MP+KACDLIRKQ +II                     V  KLKEKGIVQPTPIQVQGLPV
Sbjct: 127 MPRKACDLIRKQWHIIVDGDDIPPPIKNFKDMRLPEPVLKKLKEKGIVQPTPIQVQGLPV 186

Query: 61  ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS-EGPFGLTICPSRELARQTY 120
           ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVS EGPFGL ICPSRELARQTY
Sbjct: 187 ILSGRDMIGIAFTGSGKTLVFVLPLIMIALQEEVMMPIVSGEGPFGLIICPSRELARQTY 246

Query: 121 EVVEQFLIPMKEAGYPELKPLLCIGGVDTRSQIEVVKKNVHIVVATPGRLKDM------- 180
           EVVEQFLIPMKEAGYPEL+PLLCIGGVD RSQIE+VKK VHIVVATPGRLKDM       
Sbjct: 247 EVVEQFLIPMKEAGYPELRPLLCIGGVDMRSQIEIVKKGVHIVVATPGRLKDMLAKKKMN 306

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 307 LDNCRYLTLDEADRLVDLGFEDDIREVFDHFKAQRQTLLFSATMPTKIQNFARSALVKPV 366

Query: 241 -----------------------------------------FIFCENKADVDDIHEYLLL 300
                                                     IFCENKADVDDIHEYLLL
Sbjct: 367 TVNVGRAGAANLDVIQEVEYVKQEAKIVYLLECLQKTPPPVLIFCENKADVDDIHEYLLL 426

Query: 301 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 343
           KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE
Sbjct: 427 KGVEAVAIHGGKDQEEREYAISSFKASKKDVLVATDVASKGLDFPDIQHVINYDMPAEIE 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q9LU46|RH35_ARATH1.4e-15563.98DEAD-box ATP-dependent RNA helicase 35 OS=Arabidopsis thaliana OX=3702 GN=RH35 P... [more]
sp|Q0E3X4|RH35A_ORYSJ3.5e-15165.05DEAD-box ATP-dependent RNA helicase 35A OS=Oryza sativa subsp. japonica OX=39947... [more]
sp|Q9SZB4|RH43_ARATH1.1e-14459.75Putative DEAD-box ATP-dependent RNA helicase 43 OS=Arabidopsis thaliana OX=3702 ... [more]
sp|Q5Z6G5|RH35B_ORYSJ1.8e-13159.03DEAD-box ATP-dependent RNA helicase 35B OS=Oryza sativa subsp. japonica OX=39947... [more]
sp|Q54KG1|DDX41_DICDI1.3e-10047.14Probable ATP-dependent RNA helicase ddx41 OS=Dictyostelium discoideum OX=44689 G... [more]
Match NameE-valueIdentityDescription
AT5G51280.17.6e-15763.98DEAD-box protein abstrakt, putative[more]
AT4G33370.16.1e-14659.75DEA(D/H)-box RNA helicase family protein[more]
AT5G63120.23.2e-3830.54P-loop containing nucleoside triphosphate hydrolases superfamily protein[more]
AT3G58570.14.2e-3827.82P-loop containing nucleoside triphosphate hydrolases superfamily protein[more]
AT2G33730.12.1e-3728.42P-loop containing nucleoside triphosphate hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CDK5|A0A1S3CDK5_CUCME6.2e-16970.55DEAD-box ATP-dependent RNA helicase 35 OS=Cucumis melo OX=3656 GN=LOC103499818 P... [more]
tr|A0A0A0K6A2|A0A0A0K6A2_CUCSA6.2e-16970.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G432260 PE=4 SV=1[more]
tr|A0A200Q5Y8|A0A200Q5Y8_9MAGN5.6e-16266.95Helicase OS=Macleaya cordata OX=56857 GN=BVC80_311g2 PE=4 SV=1[more]
tr|M5WR00|M5WR00_PRUPE9.5e-16266.95Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G092400 PE=4 SV=1[more]
tr|A0A1U7ZC92|A0A1U7ZC92_NELNU1.2e-16166.95DEAD-box ATP-dependent RNA helicase 35 OS=Nelumbo nucifera OX=4432 GN=LOC1045886... [more]
Match NameE-valueIdentityDescription
XP_004135864.19.3e-16970.55PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis sativus][more]
XP_008461142.19.3e-16970.55PREDICTED: DEAD-box ATP-dependent RNA helicase 35 [Cucumis melo] >XP_008461143.1... [more]
KGN45245.19.3e-16970.55hypothetical protein Csa_7G432260 [Cucumis sativus][more]
XP_022960221.12.7e-16870.13DEAD-box ATP-dependent RNA helicase 35-like [Cucurbita moschata] >XP_022960222.1... [more]
XP_023004285.12.7e-16870.13DEAD-box ATP-dependent RNA helicase 35 [Cucurbita maxima] >XP_023004286.1 DEAD-b... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005524ATP binding
GO:0008270zinc ion binding
GO:0003676nucleic acid binding
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR036875Znf_CCHC_sf
IPR011545DEAD/DEAH_box_helicase_dom
IPR001650Helicase_C
IPR001878Znf_CCHC
IPR014001Helicase_ATP-bd
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005681 spliceosomal complex
molecular_function GO:0005524 ATP binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003676 nucleic acid binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M001402Bhi04M001402mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 254..274
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 127..284
e-value: 7.1E-48
score: 164.0
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 6..108
e-value: 2.3E-5
score: 25.5
NoneNo IPR availablePANTHERPTHR24031RNA HELICASEcoord: 20..152
coord: 153..334
NoneNo IPR availablePANTHERPTHR24031:SF542SUBFAMILY NOT NAMEDcoord: 20..152
coord: 153..334
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 25..232
e-value: 1.7E-18
score: 77.5
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 37..224
score: 16.199
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 300..316
e-value: 0.0053
score: 25.3
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 300..316
e-value: 9.8E-5
score: 22.2
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 301..316
score: 9.109
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 162..243
e-value: 5.5E-33
score: 125.6
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 126..283
score: 21.718
IPR001650Helicase, C-terminalCDDcd00079HELICccoord: 130..251
e-value: 7.83255E-34
score: 121.576
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 30..157
e-value: 3.2E-29
score: 101.8
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILYSSF57756Retrovirus zinc finger-like domainscoord: 300..321
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 43..106
coord: 161..286