Bhi09M000065 (mRNA) Wax gourd

NameBhi09M000065
TypemRNA
OrganismBenincasa hispida (Wax gourd)
Description2-oxoglutarate-dependent dioxygenase, putative
Locationchr9 : 1534911 .. 1538097 (+)
Sequence length1613
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTAAAAACTTGTTTAAATTCTCTTACAAATAAGCCATCAAAACAAAATGAAAATTGGTAGCATTGAGTAAAGCTAAGGTCTTGTAGGACAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCACTATAGATTTGTCAAAATTAGAGGCAGGTGGCACAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAGAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAATTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTCTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAACTCAGAGTTTCGCTAATCTCATGTGGCCTACTAAAGAAAATAATCACTTCTGGTAATTCATTAAACACACACTTGACTTCTCTCGTCAACTATTTAGTTTCAATTTTATTCTTATGAATTCCGAAATGTCATTGAACTTCTATTTGTTTTTTGGTTCCATATAGATCATTGAAGTTTATAAAAATTTTAAAAGTTAACCAATCCATTAGATATAGAATTTAATTTAATCGCTTCAAAAAACCACTTTTAGCCCAACATAGGATGGAAGTAACAATTTAATCCATGAACTTTTTGATTTGTAACGATTTAACCCCTTTACATTCAATTCTGAATTTTAGTGCTTAACGATTTGTTCCACTACACTTTCAAATTTGTAACAATTTAATATCTATATAAAAAAATAAATGATGACTTGTTTTATTATTATTTAAAGGATGTAGACTTTTTAGCCTTATAAGTATATTGGTGTCATGCCTAATTAGTTAATTGATTGATCTACCTAATGCAAGAAATCTTATTAAATATTAACACAAATTTTTATAATAAACACTAAATAGTTATACATTTTAAAGTGTAAGAACTAAATTGTTACATATATAGTTCAAAGACTAAACTGTTGTAAACCAAAGTCTAGAGATAAAATTATTATTTCCATTGAAGTTTAGAGACCAAAAATTATATATATATATATGCAAAGGTATTGTAATTAATGTGTGGGGTGGGAGAATCGAACCTCAGACCTCAATTGATACCAATTTTATATTTAATAGGATCCTAAAACTTCAATTTAATGCCTATTAATTCCATGAATCTATATGTATGTATTGCTTTTGAATTCAGTGCATGATAAGAATGCAATGGATGTGTCAACCTAATTGAGATATCTGAATCCCTAGTGTACCTCATGATCCCTCTCTCCTATATTCACTTATTAAAAAAACAAAGTAGCTCCATGAATTTGTGAACACTGAGATTAATGATTTGACAGTGGAACTGTGGAACGTTTCTCAACGTTGGTAGCGGAATTGGAGAAGATGGTAACAAAAATGGTATTGGAAAGCTATGGACTATCGAAGCTTGTAAATGAGCCAAACATGGAGTCAGCCAATTACCTTCTTCGATGCTTCAAATACAGGGCGCCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTCACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAGTTCAATCACTTGATGATCAATGGATTGATATTCAACCCTCAGGTCATTGCTATTTTATTGTCATGGCTGGCGATGCACTCATGGTAATCCTAATTAGCCTCAATCCTTTTCTGTTTTAAGTCCTAATCGGATCATAAAAGAAATAAGTTTGTCTCGTGAAACCATGTGATTCTTAAAACATATAAATGTGAAAATATAAAAAATATCATTGAACTATATAATTGTTGGTTTCAACTATACTTTTAACTTTTAAGAATTTCATTTTGATCCTTATTAAAGTTTGAAGTTTATTTCAAAACAACTCTTGTTAAGCTTTATTTTACTGTTAAGTTCATACACATAAAAGTTTATACTTGTTTATGTGAACAAATCATATAAATACGACAAATAAAGACACATGCATGACACATTCGTGAAACATATATTATTATTTATTATCGTCTTCCCTCCTTTCAACCCTACATGATTGTTTGTTATTTTTGCTAGCCATGAATTTAACAAGAAAAGCCTTCAACAGTTATTTTCTAAGAAAAAAACAACTTCAAATATTCAAGGGTTAAAATGAAATTTTTATAAGTTCATGAGTATAATTGAAACCAGTCCCATACTTCAGGAGTATTTTTGATAATTTAACATTTCTAAAACGTAAATGTGAACTTTAAAACGTAAGAACTAAACTTATAATTGAACCGTTGATTGTATTATTATTGATGGGGTGTAGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCACCGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACGATTGCTGTGCCTGAAGAGCTTGTGGATGATGCCAATCCACTACGATACAAGCCATTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGAATCAACAAAGCATCCATCATCAACAATCCTATATTTCACCTTTCCATAATCGTCAAATTTGTTGTTATAGGCTCAAAATTTTGAGTGGGATTCACTCATCTATTAAGCCTACAATAAAGGTTATGTTTCCCTTTCTTTCTTCTATAACTTCAATCGTATGTGAATTACTATTCATATAATTTGACTTTGATTAAAAAATAAGCATGTTCTTTGATAATCATTAGATAAAGTCTTTTTGCTCTTTCTCTCTTTACATTTTGGGTAATTCTTGGCCTTCAATAGAGTATCCATTAGTGTTCTTCCTCACATTGTGTAGCTTTTGTCACACTTTTGATTTCATATTCTTCAACCAATTCCAGAGATCCATATCCGATTTGTGTTTTTAAGTTTTCTTCCAATTCCTTTCCTTTCTCTTCAGTGGAGAGGAAACCAACTTTTTCTCTCTTCTAAAAGAGGACACCATTCATGGGCTTTCAGATTTTTCAATTATTTGATGTCGGTTTTCAACCGACATTGAACGTCAACTTTAATGTCGGGTTTTACTTATTTTTTATTCTCAAGTATCCG

mRNA sequence

GTTTTAAAAACTTGTTTAAATTCTCTTACAAATAAGCCATCAAAACAAAATGAAAATTGGTAGCATTGAGTAAAGCTAAGGTCTTGTAGGACAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCACTATAGATTTGTCAAAATTAGAGGCAGGTGGCACAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAGAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAATTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTCTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAACTCAGAGTTTCGCTAATCTCATGTGGCCTACTAAAGAAAATAATCACTTCTGTGGAACTGTGGAACGTTTCTCAACGTTGGTAGCGGAATTGGAGAAGATGGTAACAAAAATGGTATTGGAAAGCTATGGACTATCGAAGCTTGTAAATGAGCCAAACATGGAGTCAGCCAATTACCTTCTTCGATGCTTCAAATACAGGGCGCCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTCACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAGTTCAATCACTTGATGATCAATGGATTGATATTCAACCCTCAGGTCATTGCTATTTTATTGTCATGGCTGGCGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCACCGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACGATTGCTGTGCCTGAAGAGCTTGTGGATGATGCCAATCCACTACGATACAAGCCATTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGAATCAACAAAGCATCCATCATCAACAATCCTATATTTCACCTTTCCATAATCGTCAAATTTGTTGTTATAGGCTCAAAATTTTGAGTGGGATTCACTCATCTATTAAGCCTACAATAAAGGTTATGTTTCCCTTTCTTTCTTCTATAACTTCAATCGTATGTGAATTACTATTCATATAATTTGACTTTGATTAAAAAATAAGCATGTTCTTTGATAATCATTAGATAAAGTCTTTTTGCTCTTTCTCTCTTTACATTTTGGGTAATTCTTGGCCTTCAATAGAGTATCCATTAGTGTTCTTCCTCACATTGTGTAGCTTTTGTCACACTTTTGATTTCATATTCTTCAACCAATTCCAGAGATCCATATCCGATTTGTGTTTTTAAGTTTTCTTCCAATTCCTTTCCTTTCTCTTCAGTGGAGAGGAAACCAACTTTTTCTCTCTTCTAAAAGAGGACACCATTCATGGGCTTTCAGATTTTTCAATTATTTGATGTCGGTTTTCAACCGACATTGAACGTCAACTTTAATGTCGGGTTTTACTTATTTTTTATTCTCAAGTATCCG

Coding sequence (CDS)

ATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCACTATAGATTTGTCAAAATTAGAGGCAGGTGGCACAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAGAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAATTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTCTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAACTCAGAGTTTCGCTAATCTCATGTGGCCTACTAAAGAAAATAATCACTTCTGTGGAACTGTGGAACGTTTCTCAACGTTGGTAGCGGAATTGGAGAAGATGGTAACAAAAATGGTATTGGAAAGCTATGGACTATCGAAGCTTGTAAATGAGCCAAACATGGAGTCAGCCAATTACCTTCTTCGATGCTTCAAATACAGGGCGCCTGAGAAAGATGAAACCGATGTTGGTTTGCATTCCCATACAGATCTCACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGTCTTCAAGTTCAATCACTTGATGATCAATGGATTGATATTCAACCCTCAGGTCATTGCTATTTTATTGTCATGGCTGGCGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCACCGAGTGATAATGAGAGGAGAGGAAACAAGATACTCAATTGGGATGTTTTCATTCAAGAATGGGACGATTGCTGTGCCTGAAGAGCTTGTGGATGATGCCAATCCACTACGATACAAGCCATTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGA

Protein sequence

MVNSEAKAKVLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNVSRIKDYCGI
BLAST of Bhi09M000065 vs. Swiss-Prot
Match: sp|Q945B6|AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 7.9e-57
Identity = 119/300 (39.67%), Postives = 176/300 (58.67%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP ID S      G+ +W     ++   LE++GCF   +D++S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFEL 73

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           P+ T+ +N S KP+HGY        LYES+GID+   L+    F   +WP   N     T
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKY-----RAPEKDETD 190
           +  FS  + EL+ MV +M++ES+G+   ++E ++ S  YL R  KY              
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYXXXXXXXXXXXXXK 193

Query: 191 VGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIR 250
           +GL SHTD    +I+HQ+ + GL+V++ DD+WI ++PS     +VM GD+L A  N R+ 
Sbjct: 194 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRLH 253

Query: 251 SCRHRVIMRGEETRYSIGMFSF-KNGTIA-VPEELVDDANPLRYKPFNHYDFLTYDKAKA 303
           S  HRVIM G++TRYS G+FS  K G I   PEELVD  +P  +KPF + DFL + + +A
Sbjct: 254 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 307

BLAST of Bhi09M000065 vs. Swiss-Prot
Match: sp|Q944Z5|AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 119/299 (39.80%), Postives = 177/299 (59.20%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP ID S      G+ +W     ++   LE++GCF   +D++S+EL+ S+FEA ++LF L
Sbjct: 7   LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 66

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           P+ T+ +N S K +HGY        LYES+GI++   L+    F   +WP   N     T
Sbjct: 67  PIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 126

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKY----RAPEKDETDV 190
           +  FS  + EL+ MV +M++ES+G+ K ++E ++ S  YL R  KY           T +
Sbjct: 127 IHLFSEQLVELDLMVRRMIMESFGIEKYIDE-HLNSTYYLTRLMKYTXXXXXXXXXXTKL 186

Query: 191 GLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRS 250
           GL SHTD    +I+HQ+ + GL+V++ DD+WI ++PS     +VM GD+L A  N R+ S
Sbjct: 187 GLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRLHS 246

Query: 251 CRHRVIMRGEETRYSIGMFSF-KNGTIA-VPEELVDDANPLRYKPFNHYDFLTYDKAKA 303
             HRVIM G++TRYS G+FS  K G I   PEELVD  +P  +KPF + DFL + + +A
Sbjct: 247 PYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 299

BLAST of Bhi09M000065 vs. Swiss-Prot
Match: sp|Q9ZTA3|AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.0e-56
Identity = 118/301 (39.20%), Postives = 176/301 (58.47%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP ID S      G+ +W     ++   LE++GCF   +D++S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           P+ T+ +N S KP+HGY        LYES+GI++   L+    F   +WP   N     T
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKY------RAPEKDET 190
           +  FS  + EL+ MV +M++ES+G+   ++E ++ S  YL R  KY              
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYXXXXXXXXXXXXXX 193

Query: 191 DVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERI 250
            +GL SHTD    +I+HQ+ + GL+V++ DD+WI ++PS     +VM GD+L A  N R+
Sbjct: 194 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRL 253

Query: 251 RSCRHRVIMRGEETRYSIGMFSF-KNGTIA-VPEELVDDANPLRYKPFNHYDFLTYDKAK 303
            S  HRVIM G++TRYS G+FS  K G I   PEELVD  +P  +KPF + DFL + + +
Sbjct: 254 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 308

BLAST of Bhi09M000065 vs. Swiss-Prot
Match: sp|Q945B5|AOP2V_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.2e-41
Identity = 116/427 (27.17%), Postives = 189/427 (44.26%), Query Frame = 0

Query: 11  LPTIDLS-KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP I+L+ K    G+ +W     ++R  LE+FGCF   YD+VSLEL  SI +  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKE-NNHFCG 130
           P+ET+ +N   KPY GY    +   L ES+GI N   L+    F   +WP  + N +   
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPDGDGNENISK 127

Query: 131 TVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAP---------- 190
           T++ F+  + E++ MV +MV+ES+G+ K +++ +++S  Y +R  KY AP          
Sbjct: 128 TIQLFAEKLVEIDVMVRRMVMESFGIEKYIDD-HLKSTEYRMRLMKYIAPPEGDANTTVD 187

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 188 DYADLLAKLNIDGVEPNVGVKVNADISDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 247

Query: 251 ----------------------------------------------EKDETDVGLHSHTD 310
                                                         + +E  +GL  HTD
Sbjct: 248 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATSGDDVEANDDNEEKKLGLPCHTD 307

Query: 311 LTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHRV-I 317
              F+++ QH I GL+V++ D++WI ++PS +  FIV+AGD+L A  N RIR+  HRV +
Sbjct: 308 KNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPNT-FIVIAGDSLCALMNGRIRAPYHRVRV 367

BLAST of Bhi09M000065 vs. Swiss-Prot
Match: sp|Q944Z9|AOP2L_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.7e-41
Identity = 115/427 (26.93%), Postives = 189/427 (44.26%), Query Frame = 0

Query: 11  LPTIDLS-KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP I+L+ K    G+ +W     ++R  L++FGCF   YD+VSLEL  SI +  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALQDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKE-NNHFCG 130
           P+ET+ +N   KPY GY    +   L ES+GI N   L+    F   +WP  + N +   
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENINEFTQQLWPHGDGNENISK 127

Query: 131 TVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAP---------- 190
           T++ F+  + E++ MV +MV+ES+G+ K +++ +++S  Y +R  KY AP          
Sbjct: 128 TIQLFAEKLVEIDVMVRRMVMESFGIEKYIDD-HLKSTEYRMRLMKYIAPPEGDANTTVD 187

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 188 DYADLLAKLNIDGVEPNVGVKVNADISDDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 247

Query: 251 ----------------------------------------------EKDETDVGLHSHTD 310
                                                         + +E  +GL  HTD
Sbjct: 248 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATSGDDVEANDDNEEKKLGLPCHTD 307

Query: 311 LTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHRV-I 317
              F+++ QH I GL+V++ D++WI ++PS +  FIV+AGD+L A  N RIR+  HRV +
Sbjct: 308 KNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPNT-FIVIAGDSLCALMNGRIRAPYHRVRV 367

BLAST of Bhi09M000065 vs. TAIR10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 288.9 bits (738), Expect = 3.8e-78
Identity = 141/312 (45.19%), Postives = 198/312 (63.46%), Query Frame = 0

Query: 7   KAKVLPTIDLSKLE--AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATK 66
           K   +P +DL+  +     T  W    +E    LEE+GCF  VYD V+ +L +SIF A +
Sbjct: 6   KTTKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAE 65

Query: 67  ELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENN 126
           ELF LP ET+ KN +EKPYHGY G   V+PL+E +G+D     +  Q F +LMWP + N+
Sbjct: 66  ELFDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGND 125

Query: 127 HFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETD 186
            FC TV  FS  VAEL+++V +M+ E+YG+ K   E ++ S  YLL+  KY AP +  + 
Sbjct: 126 RFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHY-ESHVGSKTYLLKFLKYLAPPESISM 185

Query: 187 VGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIR 246
                HTD TF SI+HQ+ ++GL+V+S D +WI +Q     Y +VMAGD  M WSN+RIR
Sbjct: 186 PAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSY-VVMAGDISMGWSNDRIR 245

Query: 247 SCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASH 306
           SC HRV M G++TRY++G+FSF    +++PEELVDD +PL YKPF++   + +   K   
Sbjct: 246 SCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGR 305

Query: 307 QNVSRIKDYCGI 317
           +  S +K YCGI
Sbjct: 306 EANSTLKAYCGI 314

BLAST of Bhi09M000065 vs. TAIR10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 279.3 bits (713), Expect = 3.0e-75
Identity = 134/307 (43.65%), Postives = 204/307 (66.45%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSV-VYDRVSLELHNSIFEATKELFH 70
           +PT+D S+ +   GT+ W    + +R  LEE+GCF + + D+  L+L + +F +  +LF 
Sbjct: 5   IPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLFD 64

Query: 71  LPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCG 130
           LP +T++KN  +KP +GY G    LPL+ES+GIDN   L+AT+SF  LMWP + N HF  
Sbjct: 65  LPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHFSE 124

Query: 131 TVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLH 190
            + +++   AEL++MVT+MV +SY + K   +P +ES  YLLR  K RAP  +   +G  
Sbjct: 125 CLYKYAEFAAELDQMVTRMVFQSYNVEKYY-DPYIESTTYLLRVLKNRAPNNENPTLGFV 184

Query: 191 SHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRH 250
           +HTD +F +I+HQ  ++GL++++ + + I+I  S    F+V+AGDALMAWSN+R+ S RH
Sbjct: 185 THTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPRH 244

Query: 251 RVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNVS 310
           +V++ GE  RYS+GMF+F NGT+ VPEEL+D  +PL YKPF+H   L + +    +++  
Sbjct: 245 QVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSEC 304

Query: 311 RIKDYCG 316
            IK YCG
Sbjct: 305 PIKAYCG 309

BLAST of Bhi09M000065 vs. TAIR10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 270.4 bits (690), Expect = 1.4e-72
Identity = 131/309 (42.39%), Postives = 201/309 (65.05%), Query Frame = 0

Query: 11  LPTIDLS-KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP ID S K    G  EW L   +++  L+++G F   +DR+  EL  S+F A +ELF L
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 71  PLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCGT 130
           PL+T+++N S+KP+HGY G Y ++PLYESMGID+    +   +F   +WP + N  F  T
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFSTT 131

Query: 131 VERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLHS 190
           ++ FS  ++EL+  + +M++ES+GL K ++E ++ S NYLLR  KY+ P+ +ET VGL++
Sbjct: 132 IQSFSKKLSELDITIRRMIMESFGLDKYIDE-HLHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 191 HTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRHR 250
           HTD    +I++Q+ + GL+VQ+ D  WI ++P+    F VM GD+L A  N R+ S  HR
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDS-FTVMIGDSLYALLNGRLHSPYHR 251

Query: 251 VIMRGEETRYSIGMFSFKNG--TIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNV 310
           V+M G ETRYS+G+FS       ++ P+ELVD+ +P  +KPF+H +FL +   +A  ++ 
Sbjct: 252 VMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRSQ 311

Query: 311 SRIKDYCGI 317
           S +K YCGI
Sbjct: 312 SALKTYCGI 317

BLAST of Bhi09M000065 vs. TAIR10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 238.4 bits (607), Expect = 5.9e-63
Identity = 124/314 (39.49%), Postives = 187/314 (59.55%), Query Frame = 0

Query: 11  LPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKELFHL 70
           LP +D S  +   GT  W     ++R  +E  G F   +  VS +L +++    KE+++L
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 71  PLETRIKNTSEKPYHGYFG-GYSVLPLYESMGIDNPIELQATQSFANLMWPTKENNHFCG 130
           P + +IKN + K  HGY         ++ES+GID   ELQA + F+ L+WP + N+ FC 
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP-QGNDPFCQ 128

Query: 131 TVERFSTLVAELEKMVTKMVLESYGL-SKLVNEPNMESANYLLRCFKYRAPEKDETDVGL 190
           T   ++  +AEL++ V +M+ ESYG+  K  +  + ES  YLLR   YR  +  E + G 
Sbjct: 129 TTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGF 188

Query: 191 HSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCR 250
            SHTD +F SI+HQ+ + GLQ++++  QW+   PS    F+V++G  L AWSN+RI++C 
Sbjct: 189 VSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPS-PTRFVVLSGMGLTAWSNDRIKACY 248

Query: 251 HRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTY-----DKAKA 310
           H+V+M  +E RYS+G FSF  GTI  PEELVDD +PLRY PF H   L +     +  K 
Sbjct: 249 HKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYINSLKK 308

Query: 311 SHQNVSRIKDYCGI 317
           S +++ +I  YCG+
Sbjct: 309 SSEDLLQI--YCGV 318

BLAST of Bhi09M000065 vs. TAIR10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 237.7 bits (605), Expect = 1.0e-62
Identity = 132/317 (41.64%), Postives = 182/317 (57.41%), Query Frame = 0

Query: 7   KAKVLPTIDLS-KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEATKE 66
           + + LP I+LS K     TE W      +R  +E  G F   Y+     LH SI EA KE
Sbjct: 5   ETRELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKE 64

Query: 67  LFHLPLETRIKNTSEKPYHGYFGGYS-VLPLYESMGIDNPIELQATQSFANLMWPT--KE 126
           L  LP+E ++KN + K  HGY    S   P++E +GID   ++Q  + F+ LMWP    +
Sbjct: 65  LLDLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDD 124

Query: 127 NNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDE 186
           N+ FC TV  ++ + AELE++V +M+ ESY + K   E  +    YLLR  KYR     E
Sbjct: 125 NDRFCETVHAYAKMQAELEQLVIRMLFESYNVEK-YTEKYIGGTRYLLRLLKYRRLPNGE 184

Query: 187 TDVGLHSHTDLTFFSIIHQHLISGLQVQS-LDDQWIDIQPSGHCYFIVMAGDALMAWSNE 246
            +    SHTD +F SI+HQ+ I+GL ++S  +D W    PS    F+V+AGDA+MAWSN+
Sbjct: 185 PNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPS-PTRFVVIAGDAIMAWSND 244

Query: 247 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAK 306
           RI++C H+V M   E RYS+G FSF+ G I+ PEE+VD  +PL Y PF+H   L Y +  
Sbjct: 245 RIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETL 304

Query: 307 ASHQNVSR--IKDYCGI 317
             H    R   K YCGI
Sbjct: 305 EVHLKAHRTMTKAYCGI 319

BLAST of Bhi09M000065 vs. TrEMBL
Match: tr|A0A1S3CDV2|A0A1S3CDV2_CUCME (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 7.0e-159
Identity = 271/314 (86.31%), Postives = 291/314 (92.68%), Query Frame = 0

Query: 5   EAKAK-VLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEAT 64
           EAK+K V+PTIDLSKLE  GT+EWGLACKEMR GLEEFGCFSVVYD+VSLELHNSI EAT
Sbjct: 4   EAKSKVVIPTIDLSKLEV-GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63

Query: 65  KELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKEN 124
           KELFHLP+ETR+KNTSEKPYHGYFGGYS LPLYESM IDNPI+L++TQSFANLMWPT++N
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDN 123

Query: 125 NHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDET 184
           NHFC TVERFSTLVA+LEKMVTKMVLESYG+SK+VNEP MES NYLLR FKYR  EKDET
Sbjct: 124 NHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDET 183

Query: 185 DVGLHSHTDLTFFSIIHQHLISGLQVQSL-DDQWIDIQPSGHCYFIVMAGDALMAWSNER 244
           DVGLHSHTDLTFFSIIHQHLISGLQ+QSL DDQW+D+ PS HC FIVMAGDALMAWSNER
Sbjct: 184 DVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNER 243

Query: 245 IRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKA 304
           IRSCRHRVIMRGEETRYSIGMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLTYDKA A
Sbjct: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANA 303

Query: 305 SHQNVSRIKDYCGI 317
           SH  +SRIKDYCG+
Sbjct: 304 SHNTISRIKDYCGL 316

BLAST of Bhi09M000065 vs. TrEMBL
Match: tr|A0A0A0K8Y2|A0A0A0K8Y2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 4.7e-107
Identity = 192/239 (80.33%), Postives = 212/239 (88.70%), Query Frame = 0

Query: 5   EAKAK-VLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEAT 64
           EAK+K V+P IDLSKLE  GT+EWGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIFEAT
Sbjct: 4   EAKSKVVIPIIDLSKLEE-GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEAT 63

Query: 65  KELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKEN 124
           KELFHLP+ETR+KNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ TQSFANLMWPT EN
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTEN 123

Query: 125 NHFCGTVERFSTLVAELEKMVTKMVLESYGLS-KLVNEPNMESANYLLRCFKYRAPEKDE 184
           NHFC  VERFSTLVA+LE MVTKMVLESYG+S K+V+EP MES NYLLR FKYR  EKDE
Sbjct: 124 NHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDE 183

Query: 185 TDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNE 242
            DVGLHSHTDLTF SIIHQHLISGLQ+Q LDDQW+++ PS HC F VMAGDALM  +++
Sbjct: 184 NDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMVTTHQ 241

BLAST of Bhi09M000065 vs. TrEMBL
Match: tr|A0A2P6RWJ7|A0A2P6RWJ7_ROSCH (Putative oxoglutarate/iron-dependent dioxygenase, non-heme dioxygenase domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0137181 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.4e-98
Identity = 169/316 (53.48%), Postives = 235/316 (74.37%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIF 61
           + S+ +   +P +DLSK     GT+ W LA KE++  LEE+GCF  +Y++V LELHNS F
Sbjct: 1   MGSQTQPAKVPIVDLSKENLKPGTDAWLLASKEVKYALEEYGCFEAIYNKVPLELHNSTF 60

Query: 62  EATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPT 121
              ++LF+LPLE +++NTS++PYH YFG YS LPLYES+G+DNP  L+  Q F ++MWP 
Sbjct: 61  SLLEDLFNLPLEIKMQNTSDRPYHSYFGQYSFLPLYESLGVDNPTTLEGAQRFTSIMWP- 120

Query: 122 KENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEK 181
           + N+ F  +   FS LVAEL++MVTKMV E+YG+ +L  EP+M S  YLLRCFKYRAP+ 
Sbjct: 121 EGNDEFRESAHSFSKLVAELDEMVTKMVFENYGVERLY-EPHMASTTYLLRCFKYRAPQL 180

Query: 182 DETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSN 241
           +ETD+GLH H D TF SI+HQ+ I+GLQ+++ D QWID++PS    F+VMAGDA MAWSN
Sbjct: 181 NETDMGLHPHKDKTFISILHQNEINGLQIKTKDGQWIDVEPSPSS-FLVMAGDAFMAWSN 240

Query: 242 ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKA 301
           +R  SC H+VIM+ ++TRYS+G+F+F +G + VPEELVD+ +PL Y+PF+H+ FL ++K 
Sbjct: 241 DRAHSCDHQVIMKEKKTRYSMGLFAFNSGILQVPEELVDEKHPLLYRPFDHFGFLVFNKT 300

Query: 302 KASHQNVSRIKDYCGI 317
               ++   IKDYCG+
Sbjct: 301 PEGMKSECPIKDYCGV 313

BLAST of Bhi09M000065 vs. TrEMBL
Match: tr|M5WSX6|M5WSX6_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G076200 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.1e-94
Identity = 167/316 (52.85%), Postives = 226/316 (71.52%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLSKLE-AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIF 61
           + SE + K LP IDLS      GTE W LA K ++  LEE+GCF  V+ +V L+LHNSIF
Sbjct: 1   MGSETQPK-LPVIDLSNENLKPGTEAWVLASKHVKYALEEYGCFEAVFHKVPLQLHNSIF 60

Query: 62  EATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPT 121
              ++LF LP+ET+ + TSE+PYH YFG YS LPLYES+G+DNP  ++  + F  +MWP 
Sbjct: 61  SVVEDLFDLPIETKRQQTSERPYHSYFGQYSFLPLYESLGVDNPTTVEGAKGFTKIMWPA 120

Query: 122 KENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEK 181
             N+HFC + + FS LVAEL++MVT+MV + YG+ +L  + +M S  YLLRCFKYR  ++
Sbjct: 121 -GNDHFCESAQSFSKLVAELDQMVTRMVFDIYGVERLY-DSHMASTTYLLRCFKYREQKE 180

Query: 182 DETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSN 241
           +ET+VGL  H D TF SI+HQ  + GLQV+S D QWI+ +PS    F+VMAGDA MAWSN
Sbjct: 181 NETNVGLRPHKDKTFTSILHQSQVHGLQVRSKDGQWIEAEPSPSS-FLVMAGDAFMAWSN 240

Query: 242 ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKA 301
           +R+RSC H+VIM+  +TRYS+G+FSF +G + VPEEL DD +PL YKPF+H+ FL +D+ 
Sbjct: 241 DRVRSCDHQVIMKENKTRYSVGLFSFNSGILQVPEELTDDQHPLLYKPFDHFSFLRFDQT 300

Query: 302 KASHQNVSRIKDYCGI 317
             + ++   IK YCG+
Sbjct: 301 PEAKKSECPIKAYCGV 312

BLAST of Bhi09M000065 vs. TrEMBL
Match: tr|A0A1S3CDV1|A0A1S3CDV1_CUCME (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 7.9e-94
Identity = 171/317 (53.94%), Postives = 221/317 (69.72%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLS--KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61
           + SE   + LP I+ S  K++A G+ EW    K++R  LEE+GCF   Y+ V  EL   +
Sbjct: 1   MGSETMEERLPVINFSDEKVKAEGSVEWRRTSKKVREALEEYGCFIAKYEGVGKELREEV 60

Query: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121
            +A KELF LP+E +  N  EKP++GY G    LPL+ES+GID+       QSF++LMWP
Sbjct: 61  LKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNGVQSFSHLMWP 120

Query: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181
            + N+HF  T+  F+    EL+KMVTKM+ ESYG+ K  N  + +S  YLLR  K +AP+
Sbjct: 121 -EGNHHFSATLHSFAKRAEELDKMVTKMIFESYGVEKYYNS-HADSITYLLRILKNKAPQ 180

Query: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWS 241
             +  + L  HTD +F ++I+Q  I+GL++++ + QWI +  S    F+V+AGDA  AWS
Sbjct: 181 SPDPTLCLVDHTDKSFTTMIYQDHINGLELKTKNGQWIQVDNSSPSSFVVIAGDAFKAWS 240

Query: 242 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDK 301
           NERIRSCRHRVIMRGEETRYSIGMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLTYDK
Sbjct: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDK 300

Query: 302 AKASHQNVSRIKDYCGI 317
           A ASH  +SRIKDYCG+
Sbjct: 301 ANASHNTISRIKDYCGL 315

BLAST of Bhi09M000065 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 568.9 bits (1465), Expect = 1.1e-158
Identity = 271/314 (86.31%), Postives = 291/314 (92.68%), Query Frame = 0

Query: 5   EAKAK-VLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEAT 64
           EAK+K V+PTIDLSKLE  GT+EWGLACKEMR GLEEFGCFSVVYD+VSLELHNSI EAT
Sbjct: 4   EAKSKVVIPTIDLSKLEV-GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63

Query: 65  KELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKEN 124
           KELFHLP+ETR+KNTSEKPYHGYFGGYS LPLYESM IDNPI+L++TQSFANLMWPT++N
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDN 123

Query: 125 NHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDET 184
           NHFC TVERFSTLVA+LEKMVTKMVLESYG+SK+VNEP MES NYLLR FKYR  EKDET
Sbjct: 124 NHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDET 183

Query: 185 DVGLHSHTDLTFFSIIHQHLISGLQVQSL-DDQWIDIQPSGHCYFIVMAGDALMAWSNER 244
           DVGLHSHTDLTFFSIIHQHLISGLQ+QSL DDQW+D+ PS HC FIVMAGDALMAWSNER
Sbjct: 184 DVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNER 243

Query: 245 IRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKA 304
           IRSCRHRVIMRGEETRYSIGMFSFKNGTI VP+  VDDANPLRYKPFNHYDFLTYDKA A
Sbjct: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANA 303

Query: 305 SHQNVSRIKDYCGI 317
           SH  +SRIKDYCG+
Sbjct: 304 SHNTISRIKDYCGL 316

BLAST of Bhi09M000065 vs. NCBI nr
Match: XP_004136073.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 550.1 bits (1416), Expect = 5.1e-153
Identity = 262/314 (83.44%), Postives = 284/314 (90.45%), Query Frame = 0

Query: 5   EAKAK-VLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEAT 64
           EAK+K V+P IDLSKLE  GT+EWGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIFEAT
Sbjct: 4   EAKSKVVIPIIDLSKLEE-GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEAT 63

Query: 65  KELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKEN 124
           KELFHLP+ETR+KNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ TQSFANLMWPT EN
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTEN 123

Query: 125 NHFCGTVERFSTLVAELEKMVTKMVLESYGLS-KLVNEPNMESANYLLRCFKYRAPEKDE 184
           NHFC  VERFSTLVA+LE MVTKMVLESYG+S K+V+EP MES NYLLR FKYR  EKDE
Sbjct: 124 NHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDE 183

Query: 185 TDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNER 244
            DVGLHSHTDLTF SIIHQHLISGLQ+Q LDDQW+++ PS HC F VMAGDALMAWSNER
Sbjct: 184 NDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNER 243

Query: 245 IRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKA 304
           IRSCRHRVIMRGEETRYSIGMFSFKNG I VP+E VDDANPLRYKPF+HYDFLTYDKA A
Sbjct: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANA 303

Query: 305 SHQNVSRIKDYCGI 317
           SH+ +SRIKDYCG+
Sbjct: 304 SHKTISRIKDYCGL 316

BLAST of Bhi09M000065 vs. NCBI nr
Match: XP_022992484.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 492.7 bits (1267), Expect = 9.6e-136
Identity = 231/317 (72.87%), Postives = 268/317 (84.54%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLSKLEA--GGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61
           + SE +A+V P IDLSKLE    GTE WG  CK MR+GLEEFGCF VV++ VSLELHNS 
Sbjct: 1   MGSETQARV-PVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSF 60

Query: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121
           F A  ELF LP+ETRIKNTSEK YHGYFG YS LPLYESM I+NP +L A QSFANLMWP
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWP 120

Query: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181
            + NNHF  T++RFSTL+A+L+K++T+MVLESYG++K + + N++S   LLR FKYR PE
Sbjct: 121 AQGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPE 180

Query: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWS 241
           + ETDVGLH+HTDLTFFSIIHQHLI GLQ+QSLDDQWID++PSGHC F+VMAGDALMAWS
Sbjct: 181 EHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWS 240

Query: 242 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDK 301
           N+RIRSCRHRV M+ EETRYS GMFSFKNGTI+VPEELVDDANPLRYKPFNH+DFLTYDK
Sbjct: 241 NDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDK 300

Query: 302 AKASHQNVSRIKDYCGI 317
           AK S + + RI+DYC I
Sbjct: 301 AKGSKKFLYRIRDYCSI 316

BLAST of Bhi09M000065 vs. NCBI nr
Match: XP_022953943.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 488.0 bits (1255), Expect = 2.4e-134
Identity = 233/317 (73.50%), Postives = 265/317 (83.60%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLSKLE--AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61
           + SE +A V P IDLSKLE    GT+ WG  CK MR+GLEEFGCF VV++ VSLELHNS 
Sbjct: 1   MGSETQAGV-PVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSF 60

Query: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121
           F A  ELF LP+ETRIKNTSEK YHGYFG YS LPLYESM IDNP +L A QSFANLMWP
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWP 120

Query: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181
            + NN F  T++RFSTLVA+L+K+VT+MVLESYG+SK + + N++S   LLR FKYR PE
Sbjct: 121 DQGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPE 180

Query: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWS 241
           + ETDVGLH+HTDLTFFSIIHQHLI GLQ+QSLDDQWID++PS HC FIVMAGDALMAWS
Sbjct: 181 QHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWS 240

Query: 242 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDK 301
           N+RIRSCRHRV M+ EETRYS GMFSFKNGTI+VPEELVDDANPLRYKPFNHYDFLT+DK
Sbjct: 241 NDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDK 300

Query: 302 AKASHQNVSRIKDYCGI 317
           AK S + + RI+DYC I
Sbjct: 301 AKGSKKFLYRIRDYCSI 316

BLAST of Bhi09M000065 vs. NCBI nr
Match: XP_023547382.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.8 bits (1244), Expect = 4.5e-133
Identity = 228/317 (71.92%), Postives = 266/317 (83.91%), Query Frame = 0

Query: 2   VNSEAKAKVLPTIDLSKLE--AGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61
           + SE +A+V P IDLSKLE    G++ WG  CK MR+GLEEFGCF VV++ VSLE+HNS 
Sbjct: 1   MGSETQARV-PVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSF 60

Query: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121
           F A  ELF LP+ETRIKNTSEK YHGYFG YS LPLYESM IDNP +L A QSFANLMWP
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWP 120

Query: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181
            + N+ F  T++RFSTL+A+L+K+VT+MVLESYG+SK + + N++S   LLR FKYR PE
Sbjct: 121 DQGNDRFSETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPE 180

Query: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWS 241
           + ETDVGLH+HTDLTFFSIIHQHLI GLQ+QSLDDQWID++PS HC F+VMAGDALMAWS
Sbjct: 181 QHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFVVMAGDALMAWS 240

Query: 242 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDK 301
           N+RIRSCRHRV M+ EETRYS GMFSFKNGTI+VPEELVDDANPLRYKPFNHYDFLT+DK
Sbjct: 241 NDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDK 300

Query: 302 AKASHQNVSRIKDYCGI 317
           AK S + + RI+DYC I
Sbjct: 301 AKGSKKFLYRIRDYCSI 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q945B6|AOP1L_ARATH7.9e-5739.67Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
sp|Q944Z5|AOP1V_ARATH1.4e-5639.80Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
sp|Q9ZTA3|AOP1C_ARATH3.0e-5639.20Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
sp|Q945B5|AOP2V_ARATH1.2e-4127.172-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
sp|Q944Z9|AOP2L_ARATH4.7e-4126.932-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
AT1G52800.13.8e-7845.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G52790.13.0e-7543.652-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G52820.11.4e-7242.392-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G80320.15.9e-6339.492-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G15540.11.0e-6241.642-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CDV2|A0A1S3CDV2_CUCME7.0e-15986.31probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX... [more]
tr|A0A0A0K8Y2|A0A0A0K8Y2_CUCSA4.7e-10780.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1[more]
tr|A0A2P6RWJ7|A0A2P6RWJ7_ROSCH2.4e-9853.48Putative oxoglutarate/iron-dependent dioxygenase, non-heme dioxygenase domain-co... [more]
tr|M5WSX6|M5WSX6_PRUPE2.1e-9452.85Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G076200 PE=3 SV=1[more]
tr|A0A1S3CDV1|A0A1S3CDV1_CUCME7.9e-9453.94probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumi... [more]
Match NameE-valueIdentityDescription
XP_008461057.11.1e-15886.31PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi... [more]
XP_004136073.15.1e-15383.44PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus][more]
XP_022992484.19.6e-13672.87probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima][more]
XP_022953943.12.4e-13473.50probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata][more]
XP_023547382.14.5e-13371.92probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo][more]
The following terms have been associated with this mRNA:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi09G000065Bhi09G000065gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi09M000065Bhi09M000065-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi09M000065.utr5p1Bhi09M000065.utr5p1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi09M000065.exon1Bhi09M000065.exon1exon
Bhi09M000065.exon2Bhi09M000065.exon2exon
Bhi09M000065.exon3Bhi09M000065.exon3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi09M000065cds.Bhi09M000065CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi09M000065.utr3p1Bhi09M000065.utr3p1three_prime_UTR


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 175..266
e-value: 8.6E-16
score: 58.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 165..269
score: 9.856
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 11..88
e-value: 6.6E-11
score: 42.9
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 1..306
e-value: 2.6E-56
score: 193.3
NoneNo IPR availablePANTHERPTHR10209:SF1822-OXOGLUTARATE 2OG AND FE II -DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..315
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 8..315
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 11..297