Carg26304 (gene) Silver-seed gourd

NameCarg26304
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCysteine proteinase
LocationCucurbita_argyrosperma_scaffold_399 : 35884 .. 38804 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAACATACCGATCCTCACCCATTGTGCCTCTCCTCTTCTTCCTCCTCGTAACTTTACCGTCGGCGTTGCCCTCTTCGATCATCCCCGAGAGGACGGATGAGGAAGTGATGGCGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGTTGCACAACCACTTGGGACCAGAAAGCCAGAATCGATTCCACATATTCAAGGACAATCTCAAGTACATCGATGAAATTAACGCCCAAAATTTGCCCTATCGATTAGGTTTGAACGTGTTCGCAGATCTGACGAACGAGGAGTACCGATCCAAGTACTTGGGAGCTAAAATCGCCGCTGGTTCAAGGCGAAACAAGACGAGCAGTCGCTATCAGCCTCGCCTTGGGGATGAAGTCCCGGACTCCATCGATTGGAGGGAGAAAGGAGCCGTCGCTCCCATTAAAGATCAGGGAAGTTGTGGTAAGTTCCATTTTTCTCGCCTTCTTCCAATTTCTTCATCTATAGCTTTCCTATTTTTACTCGCTTTTGCATATTTGGCGCACACAAACATACACACAAAGCGCTCTCATTCACTTAGTTTTTCAGTAATTAGTATAAATTGCAAATGGTTATAATTATGTTGAAAGATGGACTGATGATCGATCATTATAGGAGGATTGATTGTTTGTTTCTTAAATTTAGATTTCATGAATAAAATTGGCGTTCCTTCATTAATCATTAGTATAGCATAACATCTGTTTTGATAATTTTGAATTATATGTAACCATCTGACTAATTATAGGAAAGAACATGAGGGCACAGAATCTTTATGAGTTGCTCGTGGAATGGACCTGCCTTTACTTTCAACTCGCACTGGCCCCGCACTTTCTATAGAGATTTAATGATGAGATATATATTTCTTCTGAAAAATTTTAACCACGATATATTTGAATGGCTGTTTTTCTTTTCCTCCAAATATTTATGAACAGTATAGGATTCACAAAAGTTATTTCTTTACTTGCCATTCTTTCTTAATTATTATTTATAAAATATTTCGATCTTAAATCATATAATCACATGATTTGATTTTTATGTGCAACTTTTATGACTTGCTATTATATTATCTCAATGAAAGCTGAGGTTTTTACCAAATAATAATAATAATCTCAGTTGTTGATAACACCACACTCATTTTAATTATTAAATTTGTCATCGATTGTTTTAGGAAATATAGAATTAGAATGTTTGAGAGATTAAAAGTTGAAACCTTTTGTAAAGTTCTTAAAAGACGTAGAGAGAATAAACGTTTTCTTGACTTATTCTCTCTGAAGTATATCATAAATAAACTATATGAACATTGGTCGCTTCAAGTATGCTAATTGTTGAAGTTGATATGAAGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAGGCATAAACCAGATTGTTACAGGCGACTTGGTAGCATTATCAGAACAGGAGCTGGTGGATTGTGACATTTCTTACAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCAACAATGGCGGCATTGATACTGAAGAAGATTATCCTTATAAAGGCTATGATAGCACCTGCATCCAATCTAAGGTCTCTATTGCTACTCAAACTTTCTTTTTCCATTTTGAGTTGCTGAAGCTTATTTTGATATCTGATTGTCTTTATTTCTATTGTGTTTGTCTTCTTCAGAAAAATGCTAGGGTCGTTTCAATCGATGATTATGAAGACGTCCCTGTAAACAATGAAAAAGCACTGCAAAAAGCAGCAGCTAAACAGGTTGTGGCTGTTGCCATTGAAGGGGGAGGTAGAAGTTTCCAGCTATATCGATCGGTATAACATCCATTTCTATGAAGTTTTGGTTATTATTATTGTTTTTTGTACGCTTGGAGAGAGGGTCTGATTTGTTGTATGTGTATGTGTACAGGGTATATTCACTGGAAGATGTGGGACAGATTTGGATCATGGTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCGTCGATTACTGGATTGTGAGAAACTCATGGGGTGCGAGCTGGGGTGAAAGTGGCTACATCAGGATGCAACGTAACGTCGCTTCCCCAAAAGGTCTCTGTGGGATTGCAATGGAGCCTTCTTACCCCATTAAAACCGGACTAAACCCACCTAATCCAGGCCCGACTCCTCCAAGTCCGGTGGAACCCCCAACTCCGGTGAAACCCCCAACTGTATGCGATGAATATTACGACTGTCCCGCCGCAGAAACTTGCTGCTGCCTCTTCCAGTTTTCAAACACATGCTTTGTATGGGGATGCTGCCCACTGGAAAGTGCCACCTGCTGTGATGACCTTTACAGCTGCTGCCCCCATGACTACCCCGTCTGTGACGTCCGTGCTGGAACCTGCTTGAAGGTACGTGGCTTCAATTTCCATTTTCATATGCATCTTGTCAAAATGCAAGGGGTAAACGTCTGATGAAGACTGAACTTATATTTTTTTGTTGTGCAGAGCAAGAATGATGTTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGTAGCCAAACCATCGTGGGCACTTGGAGGAGGTGTGAGTGTTGGGAAGAGCAGTGCTTAAAAAGGTTGCCAGGCTTGTTACTGTTGAATCAATAACTCTCCAAGCCCTGTATTTCTCATATTGTACGCCTCCTGGCTTCGCACCTTTTAGCTCAATGGTCGTTTAAGTTAGCACACGATGACTGTATATAGGACGTATATGAACACTTGGAACAGAGACTAATTGCTGTCAAGTTATATTAGCACTCTGTCTCTGTGTCAAATCCTGAATTCAGGCCAATTGTGGCTGTCCCTTAATTATGATCTGCTGTATCATTTCATTGAATCAGTAGTCTTTCCTTCACAAAAACATGAATCAAAGACAAACTGAAAA

mRNA sequence

ATGGGAACATACCGATCCTCACCCATTGTGCCTCTCCTCTTCTTCCTCCTCGTAACTTTACCGTCGGCGTTGCCCTCTTCGATCATCCCCGAGAGGACGGATGAGGAAGTGATGGCGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGTTGCACAACCACTTGGGACCAGAAAGCCAGAATCGATTCCACATATTCAAGGACAATCTCAAGTACATCGATGAAATTAACGCCCAAAATTTGCCCTATCGATTAGGTTTGAACGTGTTCGCAGATCTGACGAACGAGGAGTACCGATCCAAGTACTTGGGAGCTAAAATCGCCGCTGGTTCAAGGCGAAACAAGACGAGCAGTCGCTATCAGCCTCGCCTTGGGGATGAAGTCCCGGACTCCATCGATTGGAGGGAGAAAGGAGCCGTCGCTCCCATTAAAGATCAGGGAAGTTGTGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAGGCATAAACCAGATTGTTACAGGCGACTTGGTAGCATTATCAGAACAGGAGCTGGTGGATTGTGACATTTCTTACAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCAACAATGGCGGCATTGATACTGAAGAAGATTATCCTTATAAAGGCTATGATAGCACCTGCATCCAATCTAAGAAAAATGCTAGGGTCGTTTCAATCGATGATTATGAAGACGTCCCTGTAAACAATGAAAAAGCACTGCAAAAAGCAGCAGCTAAACAGGTTGTGGCTGTTGCCATTGAAGGGGGAGGTAGAAGTTTCCAGCTATATCGATCGGGTATATTCACTGGAAGATGTGGGACAGATTTGGATCATGGTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCGTCGATTACTGGATTGTGAGAAACTCATGGGGTGCGAGCTGGGGTGAAAGTGGCTACATCAGGATGCAACGTAACGTCGCTTCCCCAAAAGGTCTCTGTGGGATTGCAATGGAGCCTTCTTACCCCATTAAAACCGGACTAAACCCACCTAATCCAGGCCCGACTCCTCCAAGTCCGGTGGAACCCCCAACTCCGGTGAAACCCCCAACTGTATGCGATGAATATTACGACTGTCCCGCCGCAGAAACTTGCTGCTGCCTCTTCCAGTTTTCAAACACATGCTTTGTATGGGGATGCTGCCCACTGGAAAGTGCCACCTGCTGTGATGACCTTTACAGCTGCTGCCCCCATGACTACCCCGTCTGTGACGTCCGTGCTGGAACCTGCTTGAAGAGCAAGAATGATGTTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGTAGCCAAACCATCGTGGGCACTTGGAGGAGGTGTGAGTGTTGGGAAGAGCAGTGCTTAAAAAGGTTGCCAGGCTTGTTACTGTTGAATCAATAACTCTCCAAGCCCTGTATTTCTCATATTGTACGCCTCCTGGCTTCGCACCTTTTAGCTCAATGGTCGTTTAAGTTAGCACACGATGACTGTATATAGGACGTATATGAACACTTGGAACAGAGACTAATTGCTGTCAAGTTATATTAGCACTCTGTCTCTGTGTCAAATCCTGAATTCAGGCCAATTGTGGCTGTCCCTTAATTATGATCTGCTGTATCATTTCATTGAATCAGTAGTCTTTCCTTCACAAAAACATGAATCAAAGACAAACTGAAAA

Coding sequence (CDS)

ATGGGAACATACCGATCCTCACCCATTGTGCCTCTCCTCTTCTTCCTCCTCGTAACTTTACCGTCGGCGTTGCCCTCTTCGATCATCCCCGAGAGGACGGATGAGGAAGTGATGGCGCTCTACGACCAGTGGCGTGCCAAGCACGGGAAGTTGCACAACCACTTGGGACCAGAAAGCCAGAATCGATTCCACATATTCAAGGACAATCTCAAGTACATCGATGAAATTAACGCCCAAAATTTGCCCTATCGATTAGGTTTGAACGTGTTCGCAGATCTGACGAACGAGGAGTACCGATCCAAGTACTTGGGAGCTAAAATCGCCGCTGGTTCAAGGCGAAACAAGACGAGCAGTCGCTATCAGCCTCGCCTTGGGGATGAAGTCCCGGACTCCATCGATTGGAGGGAGAAAGGAGCCGTCGCTCCCATTAAAGATCAGGGAAGTTGTGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAGGCATAAACCAGATTGTTACAGGCGACTTGGTAGCATTATCAGAACAGGAGCTGGTGGATTGTGACATTTCTTACAACGAAGGTTGCAATGGTGGCTTAATGGACTATGCCTTCGAGTTCATCATCAACAATGGCGGCATTGATACTGAAGAAGATTATCCTTATAAAGGCTATGATAGCACCTGCATCCAATCTAAGAAAAATGCTAGGGTCGTTTCAATCGATGATTATGAAGACGTCCCTGTAAACAATGAAAAAGCACTGCAAAAAGCAGCAGCTAAACAGGTTGTGGCTGTTGCCATTGAAGGGGGAGGTAGAAGTTTCCAGCTATATCGATCGGGTATATTCACTGGAAGATGTGGGACAGATTTGGATCATGGTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCGTCGATTACTGGATTGTGAGAAACTCATGGGGTGCGAGCTGGGGTGAAAGTGGCTACATCAGGATGCAACGTAACGTCGCTTCCCCAAAAGGTCTCTGTGGGATTGCAATGGAGCCTTCTTACCCCATTAAAACCGGACTAAACCCACCTAATCCAGGCCCGACTCCTCCAAGTCCGGTGGAACCCCCAACTCCGGTGAAACCCCCAACTGTATGCGATGAATATTACGACTGTCCCGCCGCAGAAACTTGCTGCTGCCTCTTCCAGTTTTCAAACACATGCTTTGTATGGGGATGCTGCCCACTGGAAAGTGCCACCTGCTGTGATGACCTTTACAGCTGCTGCCCCCATGACTACCCCGTCTGTGACGTCCGTGCTGGAACCTGCTTGAAGAGCAAGAATGATGTTTTCGGAGTGAAGGCGATGAGGCGCACTGCAGCTGTAGCCAAACCATCGTGGGCACTTGGAGGAGGTGTGAGTGTTGGGAAGAGCAGTGCTTAA

Protein sequence

MGTYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQNRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRYQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPVEPPTPVKPPTVCDEYYDCPAAETCCCLFQFSNTCFVWGCCPLESATCCDDLYSCCPHDYPVCDVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA
BLAST of Carg26304 vs. NCBI nr
Match: XP_022936812.1 (low-temperature-induced cysteine proteinase-like [Cucurbita moschata])

HSP 1 Score: 859.8 bits (2220), Expect = 4.4e-246
Identity = 463/466 (99.36%), Postives = 463/466 (99.36%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ 60
           MGT RSSPIVPLL FLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ
Sbjct: 1   MGTCRSSPIVPLLVFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ 60

Query: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120
           NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180
           QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVE INQIVTGDLVALSEQEL
Sbjct: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVESINQIVTGDLVALSEQEL 180

Query: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240
           VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE
Sbjct: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240

Query: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300
           DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX 360
           VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX
Sbjct: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX 360

Query: 361 XXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXX 420
           XXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 467
           XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA
Sbjct: 421 XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 466

BLAST of Carg26304 vs. NCBI nr
Match: XP_022977015.1 (low-temperature-induced cysteine proteinase-like [Cucurbita maxima])

HSP 1 Score: 852.0 bits (2200), Expect = 9.2e-244
Identity = 459/466 (98.50%), Postives = 461/466 (98.93%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ 60
           MGTYRSSPIV LLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPE+Q
Sbjct: 1   MGTYRSSPIVALLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPETQ 60

Query: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120
           NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180
           QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL
Sbjct: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180

Query: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240
           VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE
Sbjct: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240

Query: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300
           DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX 360
           VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIK GLNPPNPGPTPP  X
Sbjct: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKNGLNPPNPGPTPPXXX 360

Query: 361 XXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXX 420
           XXXXXXXXXXXXD+YYDCPAAETCCCLFQFSNTCFVWGC XXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXDQYYDCPAAETCCCLFQFSNTCFVWGCXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 467
           XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA
Sbjct: 421 XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 466

BLAST of Carg26304 vs. NCBI nr
Match: XP_023534814.1 (low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 2.7e-243
Identity = 457/472 (96.82%), Postives = 459/472 (97.25%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ 60
           MGTYRSSPI+ LLFFLLVTLPSAL SSIIPERTD+EVMALYDQWRAKHGKLHNHLGPESQ
Sbjct: 1   MGTYRSSPILALLFFLLVTLPSALTSSIIPERTDDEVMALYDQWRAKHGKLHNHLGPESQ 60

Query: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120
           NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180
           QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL
Sbjct: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180

Query: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240
           VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE
Sbjct: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240

Query: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300
           DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSP- 360
           VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSP 
Sbjct: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX 360

Query: 361 -----XXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXX 420
                XXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCC   XXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCPLEXXXXXXXXXXX 420

Query: 421 XXXXXXXXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 467
           XXXXXXXX V AGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA
Sbjct: 421 XXXXXXXXDVGAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 472

BLAST of Carg26304 vs. NCBI nr
Match: XP_008452589.2 (PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo])

HSP 1 Score: 680.6 bits (1755), Expect = 3.7e-192
Identity = 375/466 (80.47%), Postives = 407/466 (87.34%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ 60
           MGT++SS  + LLFFL + L +A PSSIIP+RTDEEVMALYDQWRA HGKLHN+LG E +
Sbjct: 1   MGTFQSSQTMALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPE 60

Query: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120
           NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRS+YLG + AAGSRRN+TS+RY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180
            PRLGDE+PDSIDWR KGAVAP+KDQGSCGSCWAFST+A+VEGINQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDELPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQEL 180

Query: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240
           VDCD SYNEGCNGGLMDYAFEFIINNGG+DTEEDYPY G+DS+CIQ KKNARVVSID YE
Sbjct: 181 VDCDKSYNEGCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYE 240

Query: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300
           DVPVNNEKALQKAA+KQVV+VAIEGGGRSFQLY+SGIFTGRCGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX 360
           VDYWIVRNSWGA+WGESGY+RMQRN+ SP GLCGIAMEPSYP K+G             X
Sbjct: 301 VDYWIVRNSWGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSG------PXXXXXXX 360

Query: 361 XXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXX 420
           XXXXXXXXX   DEYY CPAAETCCC+F+FS         XXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXSVCDEYYTCPAAETCCCIFEFSXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 467
           XXX       KSKND+FGVKAMRRTAA A+PSWA    V+VGKSSA
Sbjct: 421 XXXXXXXXXXKSKNDIFGVKAMRRTAAAARPSWA-HRDVTVGKSSA 459

BLAST of Carg26304 vs. NCBI nr
Match: XP_004141271.2 (PREDICTED: low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN55227.1 hypothetical protein Csa_4G641600 [Cucumis sativus])

HSP 1 Score: 678.7 bits (1750), Expect = 1.4e-191
Identity = 372/466 (79.83%), Postives = 407/466 (87.34%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ 60
           MGT++SSPI+ LLFFL + L +A PSSIIP+RTD+EVMALYDQWRAKHGKLHN+LG E +
Sbjct: 1   MGTFQSSPIMALLFFLFIALSAASPSSIIPQRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60

Query: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120
           NRFHIFKDNLK+IDEINAQNLPYRLGLNVFADLTNEEYRS+YLG K A+GSRRN+TS+RY
Sbjct: 61  NRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180
            PRLGD++PDSIDWR KGAVAP+KDQGSCGSCWAFST+A+VE INQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDDLPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEAINQIVTGDLIALSEQEL 180

Query: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240
           VDCD SYNEGCNGGLMDYAFEFII NGG+DTEEDYPY G+DS+CIQ KKNA+VV+ID YE
Sbjct: 181 VDCDRSYNEGCNGGLMDYAFEFIIENGGLDTEEDYPYYGFDSSCIQYKKNAKVVAIDGYE 240

Query: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300
           DVPVNNEKALQKA +KQVV+VAIEGGGRSFQLY+SGIFTGRCGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX 360
           VDYWIVRNSWG SWGESGY++MQRN+ASP GLCGIAMEPSYP KTG             X
Sbjct: 301 VDYWIVRNSWGGSWGESGYVKMQRNIASPTGLCGIAMEPSYPTKTG------PXXXXXXX 360

Query: 361 XXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXX 420
           XXXXXXXXX   DEYY CPAAETCCC+FQFS         XXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXSVCDEYYTCPAAETCCCIFQFSXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 467
           XXX       KSKND+FGVKAMRRTAA A+PSWA    V+VGKSSA
Sbjct: 421 XXXXXXXXXXKSKNDIFGVKAMRRTAAAARPSWA-RRDVTVGKSSA 459

BLAST of Carg26304 vs. TAIR10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein)

HSP 1 Score: 517.3 bits (1331), Expect = 9.7e-147
Identity = 281/474 (59.28%), Postives = 332/474 (70.04%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSII-----------PERTDEEVMALYDQWRAKHG 60
           MG  + SP++ LL   ++ +  A+  SII             R+D EV  +Y+ W  +HG
Sbjct: 1   MGFLKLSPMILLL--AMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHG 60

Query: 61  KL---HNHLGPESQNRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAK 120
           K     N LG E   RF IFKDNL++IDE N +NL Y+LGL  FADLTNEEYRS YLGAK
Sbjct: 61  KKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK 120

Query: 121 IAAGSRRNKTSSRYQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQ 180
                R  KTS RYQ R+GD +PDS+DWR++GAVA +KDQGSCGSCWAFSTI AVEGIN+
Sbjct: 121 --PTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINK 180

Query: 181 IVTGDLVALSEQELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQ 240
           IVTGDL++LSEQELVDCD SYN+GCNGGLMDYAFEFII NGGIDTE DYPYK  D  C Q
Sbjct: 181 IVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQ 240

Query: 241 SKKNARVVSIDDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDL 300
           ++KNA+VV+ID YEDVP N+E +L+KA A Q ++VAIE GGR+FQLY SG+F G CGT+L
Sbjct: 241 NRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTEL 300

Query: 301 DHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTG 360
           DHGV  VGYG+E G DYWIVRNSWG  WGESGYI+M RN+ +P G CGIAME SYPIK G
Sbjct: 301 DHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIKKG 360

Query: 361 LNPPN------PGPTPPSPXXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCC 420
            NPPN          PP+              D+Y+ CP + TCCCL+++   CF WGCC
Sbjct: 361 QNPPNXXXXXXXXXKPPT------------TCDKYFSCPESNTCCCLYKYGKYCFGWGCC 420

Query: 421 XXXXXXXXXXXXXXXXXXXXXXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWA 455
                     XXXXXXXXXXXXXV  GTCL SKN  F VKA++RT A+  P WA
Sbjct: 421 PLEAATCCDDXXXXXXXXXXXXXVNRGTCLMSKNSPFSVKALKRTPAI--PFWA 456

BLAST of Carg26304 vs. TAIR10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein)

HSP 1 Score: 515.8 bits (1327), Expect = 2.8e-146
Identity = 283/467 (60.60%), Postives = 332/467 (71.09%), Query Frame = 0

Query: 8   PIVPLLFFLLVTLPSALPSSIIP-----------ERTDEEVMALYDQWRAKHGKLHNHLG 67
           P + +LF  +V + SA+  SII             R++ EVM++Y+ W  KHGK  +   
Sbjct: 6   PTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNS 65

Query: 68  -PESQNRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNK 127
             E   RF IFKDNL+++DE N +NL YRLGL  FADLTN+EYRSKYLGAK+     R +
Sbjct: 66  LVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER-R 125

Query: 128 TSSRYQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVAL 187
           TS RY+ R+GDE+P+SIDWR+KGAVA +KDQG CGSCWAFSTI AVEGINQIVTGDL+ L
Sbjct: 126 TSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITL 185

Query: 188 SEQELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVS 247
           SEQELVDCD SYNEGCNGGLMDYAFEFII NGGIDT++DYPYKG D TC Q +KNA+VV+
Sbjct: 186 SEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVT 245

Query: 248 IDDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGY 307
           ID YEDVP  +E++L+KA A Q +++AIE GGR+FQLY SGIF G CGT LDHGV  VGY
Sbjct: 246 IDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGY 305

Query: 308 GSEGGVDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPN---- 367
           G+E G DYWIVRNSWG SWGESGY+RM RN+AS  G CGIA+EPSYPIK G NPPN    
Sbjct: 306 GTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKNGENPPNXXXX 365

Query: 368 --PGPTPPSPXXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXX 427
                 PP+              D YY CP + TCCCLF++   CF WGCC    XXXXX
Sbjct: 366 XXXXXKPPT------------QCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAXXXXX 425

Query: 428 XXXXXXXXXXXXXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALG 457
           XXXXXXXXXXXXXX        SKN  F VKA++R  A   P W+ G
Sbjct: 426 XXXXXXXXXXXXXXXXXXXXXXSKNSPFSVKALKRKPAT--PFWSQG 457

BLAST of Carg26304 vs. TAIR10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein)

HSP 1 Score: 429.1 bits (1102), Expect = 3.5e-120
Identity = 221/400 (55.25%), Postives = 280/400 (70.00%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFF--LLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPE 60
           M T   S  + LL F  LL++L     ++    R + E   +Y++W  ++ K +N LG E
Sbjct: 1   MATSIKSITLALLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLG-E 60

Query: 61  SQNRFHIFKDNLKYIDEINA-QNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTS 120
            + RF IFKDNLK+++E ++  N  Y +GL  FADLTN+E+R+ YL +K+   +R     
Sbjct: 61  KERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMER-TRVPVKG 120

Query: 121 SRYQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSE 180
            +Y  ++GD +PD+IDWR KGAV P+KDQGSCGSCWAFS I AVEGINQI TG+L++LSE
Sbjct: 121 EKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSE 180

Query: 181 QELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYD-STCIQSKKNARVVSI 240
           QELVDCD SYN+GC GGLMDYAF+FII NGGIDTEEDYPY   D + C   KKN RVV+I
Sbjct: 181 QELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTI 240

Query: 241 DDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYG 300
           D YEDVP N+EK+L+KA A Q ++VAIE GGR+FQLY SG+FTG CGT LDHGV  VGYG
Sbjct: 241 DGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYG 300

Query: 301 SEGGVDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIK-TGLNPPNPGPT 360
           SEGG DYWIVRNSWG++WGESGY +++RN+    G CG+AM  SYP K +G NPP   P 
Sbjct: 301 SEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVAMMASYPTKSSGSNPPK--PP 360

Query: 361 PPSPXXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCF 396
            PSP             D+   CPA  TCCCL++++  C+
Sbjct: 361 APSP----------VVCDKSNTCPAKSTCCCLYEYNGKCY 386

BLAST of Carg26304 vs. TAIR10
Match: AT4G36880.1 (cysteine proteinase1)

HSP 1 Score: 413.7 bits (1062), Expect = 1.5e-115
Identity = 213/357 (59.66%), Postives = 264/357 (73.95%), Query Frame = 0

Query: 12  LLFFLLVTLPSALPSSI-----IPE----RTDEEVMALYDQWRAKHGKLHNH---LGPES 71
           LL +++V+L S   S I     +P     RTDEEV ++Y QW A+HGK +N+   +  + 
Sbjct: 11  LLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNNNNGIINDQ 70

Query: 72  QNRFHIFKDNLKYID--EINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNK-- 131
             RF+IFKDNL++ID    N +N  Y+LGL  F DLTN+EYR  YLGA+     R  K  
Sbjct: 71  DKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTEPARRIAKAK 130

Query: 132 -TSSRYQPRL-GDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLV 191
             + +Y   + G EVP+++DWR+KGAV PIKDQG+CGSCWAFST AAVEGIN+IVTG+L+
Sbjct: 131 NVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVTGELI 190

Query: 192 ALSEQELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARV 251
           +LSEQELVDCD SYN+GCNGGLMDYAF+FI+ NGG++TE+DYPY+G+   C    KN+RV
Sbjct: 191 SLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCNSFLKNSRV 250

Query: 252 VSIDDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVV 311
           VSID YEDVP  +E AL+KA + Q V+VAIE GGR FQ Y+SGIFTG CGT+LDH V  V
Sbjct: 251 VSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGTNLDHAVVAV 310

Query: 312 GYGSEGGVDYWIVRNSWGASWGESGYIRMQRNVASPK-GLCGIAMEPSYPIKTGLNP 350
           GYGSE GVDYWIVRNSWG  WGE GYIRM+RN+A+ K G CGIA+E SYP+K   NP
Sbjct: 311 GYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEASYPVKYSPNP 367

BLAST of Carg26304 vs. TAIR10
Match: AT4G23520.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 376.3 bits (965), Expect = 2.7e-104
Identity = 185/347 (53.31%), Postives = 250/347 (72.05%), Query Frame = 0

Query: 8   PIVPLLFFLLVTLPSALPSSIIP--------ERTDEEVMALYDQWRAKHGKLHNHLGPES 67
           P+   + FLL+    + PSS +          R++EEV  ++  W +KHGK + +   E 
Sbjct: 6   PVCMTILFLLIVFVLSAPSSAMDLPATSGGHNRSNEEVEFIFQMWMSKHGKTYTNALGEK 65

Query: 68  QNRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSR 127
           + RF  FKDNL++ID+ NA+NL Y+LGL  FADLT +EYR  + G+      R  KTS R
Sbjct: 66  ERRFQNFKDNLRFIDQHNAKNLSYQLGLTRFADLTVQEYRDLFPGSP-KPKQRNLKTSRR 125

Query: 128 YQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQE 187
           Y P  GD++P+S+DWR++GAV+ IKDQG+C SCWAFST+AAVEG+N+IVTG+L++LSEQE
Sbjct: 126 YVPLAGDQLPESVDWRQEGAVSEIKDQGTCNSCWAFSTVAAVEGLNKIVTGELISLSEQE 185

Query: 188 LVDCDISYNEGCNG-GLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNA-RVVSID 247
           LVDC++  N GC G GLMD AF+F+INN G+D+E+DYPY+G   +C + +  + +V++ID
Sbjct: 186 LVDCNL-VNNGCYGSGLMDTAFQFLINNNGLDSEKDYPYQGTQGSCNRKQSTSNKVITID 245

Query: 248 DYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGS 307
            YEDVP N+E +LQKA A Q V+V ++   + F LYRS I+ G CGT+LDH + +VGYGS
Sbjct: 246 SYEDVPANDEISLQKAVAHQPVSVGVDKKSQEFMLYRSCIYNGPCGTNLDHALVIVGYGS 305

Query: 308 EGGVDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIK 345
           E G DYWIVRNSWG +WG++GYI++ RN   PKGLCGIAM  SYPIK
Sbjct: 306 ENGQDYWIVRNSWGTTWGDAGYIKIARNFEDPKGLCGIAMLASYPIK 350

BLAST of Carg26304 vs. Swiss-Prot
Match: sp|Q9FMH8|RD21B_ARATH (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 1.8e-145
Identity = 281/474 (59.28%), Postives = 332/474 (70.04%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSII-----------PERTDEEVMALYDQWRAKHG 60
           MG  + SP++ LL   ++ +  A+  SII             R+D EV  +Y+ W  +HG
Sbjct: 1   MGFLKLSPMILLL--AMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHG 60

Query: 61  KL---HNHLGPESQNRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAK 120
           K     N LG E   RF IFKDNL++IDE N +NL Y+LGL  FADLTNEEYRS YLGAK
Sbjct: 61  KKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAK 120

Query: 121 IAAGSRRNKTSSRYQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQ 180
                R  KTS RYQ R+GD +PDS+DWR++GAVA +KDQGSCGSCWAFSTI AVEGIN+
Sbjct: 121 --PTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINK 180

Query: 181 IVTGDLVALSEQELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQ 240
           IVTGDL++LSEQELVDCD SYN+GCNGGLMDYAFEFII NGGIDTE DYPYK  D  C Q
Sbjct: 181 IVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQ 240

Query: 241 SKKNARVVSIDDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDL 300
           ++KNA+VV+ID YEDVP N+E +L+KA A Q ++VAIE GGR+FQLY SG+F G CGT+L
Sbjct: 241 NRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTEL 300

Query: 301 DHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTG 360
           DHGV  VGYG+E G DYWIVRNSWG  WGESGYI+M RN+ +P G CGIAME SYPIK G
Sbjct: 301 DHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIKKG 360

Query: 361 LNPPN------PGPTPPSPXXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCC 420
            NPPN          PP+              D+Y+ CP + TCCCL+++   CF WGCC
Sbjct: 361 QNPPNXXXXXXXXXKPPT------------TCDKYFSCPESNTCCCLYKYGKYCFGWGCC 420

Query: 421 XXXXXXXXXXXXXXXXXXXXXXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWA 455
                     XXXXXXXXXXXXXV  GTCL SKN  F VKA++RT A+  P WA
Sbjct: 421 PLEAATCCDDXXXXXXXXXXXXXVNRGTCLMSKNSPFSVKALKRTPAI--PFWA 456

BLAST of Carg26304 vs. Swiss-Prot
Match: sp|P43297|RD21A_ARATH (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 5.1e-145
Identity = 283/467 (60.60%), Postives = 332/467 (71.09%), Query Frame = 0

Query: 8   PIVPLLFFLLVTLPSALPSSIIP-----------ERTDEEVMALYDQWRAKHGKLHNHLG 67
           P + +LF  +V + SA+  SII             R++ EVM++Y+ W  KHGK  +   
Sbjct: 6   PTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNS 65

Query: 68  -PESQNRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNK 127
             E   RF IFKDNL+++DE N +NL YRLGL  FADLTN+EYRSKYLGAK+     R +
Sbjct: 66  LVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER-R 125

Query: 128 TSSRYQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVAL 187
           TS RY+ R+GDE+P+SIDWR+KGAVA +KDQG CGSCWAFSTI AVEGINQIVTGDL+ L
Sbjct: 126 TSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITL 185

Query: 188 SEQELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVS 247
           SEQELVDCD SYNEGCNGGLMDYAFEFII NGGIDT++DYPYKG D TC Q +KNA+VV+
Sbjct: 186 SEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVT 245

Query: 248 IDDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGY 307
           ID YEDVP  +E++L+KA A Q +++AIE GGR+FQLY SGIF G CGT LDHGV  VGY
Sbjct: 246 IDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGY 305

Query: 308 GSEGGVDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPN---- 367
           G+E G DYWIVRNSWG SWGESGY+RM RN+AS  G CGIA+EPSYPIK G NPPN    
Sbjct: 306 GTENGKDYWIVRNSWGKSWGESGYLRMARNIASSSGKCGIAIEPSYPIKNGENPPNXXXX 365

Query: 368 --PGPTPPSPXXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXX 427
                 PP+              D YY CP + TCCCLF++   CF WGCC    XXXXX
Sbjct: 366 XXXXXKPPT------------QCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAXXXXX 425

Query: 428 XXXXXXXXXXXXXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALG 457
           XXXXXXXXXXXXXX        SKN  F VKA++R  A   P W+ G
Sbjct: 426 XXXXXXXXXXXXXXXXXXXXXXSKNSPFSVKALKRKPAT--PFWSQG 457

BLAST of Carg26304 vs. Swiss-Prot
Match: sp|P25776|ORYA_ORYSJ (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 477.6 bits (1228), Expect = 1.5e-133
Identity = 275/440 (62.50%), Postives = 323/440 (73.41%), Query Frame = 0

Query: 31  ERTDEEVMALYDQWRAKHGKLHNHLGPESQNRFHIFKDNLKYIDEINAQN----LPYRLG 90
           ER++EE   LY +W+A+HGK +N +G E + R+  F+DNL+YIDE NA        +RLG
Sbjct: 30  ERSEEEARRLYAEWKAEHGKSYNAVG-EEERRYAAFRDNLRYIDEHNAAADAGVHSFRLG 89

Query: 91  LNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRYQPRLGDEVPDSIDWREKGAVAPIKDQ 150
           LN FADLTNEEYR  YLG +     R  K S RY     + +P+S+DWR KGAVA IKDQ
Sbjct: 90  LNRFADLTNEEYRDTYLGLR-NKPRRERKVSDRYLAADNEALPESVDWRTKGAVAEIKDQ 149

Query: 151 GSCGSCWAFSTIAAVEGINQIVTGDLVALSEQELVDCDISYNEGCNGGLMDYAFEFIINN 210
           G CGSCWAFS IAAVEGINQIVTGDL++LSEQELVDCD SYNEGCNGGLMDYAF+FIINN
Sbjct: 150 GGCGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFDFIINN 209

Query: 211 GGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDVPVNNEKALQKAAAKQVVAVAIEGG 270
           GGIDTE+DYPYKG D  C  ++KNA+VV+ID YEDV  N+E +LQKA A Q V+VAIE G
Sbjct: 210 GGIDTEDDYPYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQKAVANQPVSVAIEAG 269

Query: 271 GRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYIRMQRNV 330
           GR+FQLY SGIFTG+CGT LDHGV  VGYG+E G DYWIVRNSWG SWGESGY+RM+RN+
Sbjct: 270 GRAFQLYSSGIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGKSWGESGYVRMERNI 329

Query: 331 ASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPXXXXXXXXXXXXXDEYYDCPAAETCCC 390
            +  G CGIA+EPSYP+K G NP      P   XXXXXXXXXXX  D YY CP + TCCC
Sbjct: 330 KASSGKCGIAVEPSYPLKKGENP------PXXXXXXXXXXXXXXVCDNYYTCPDSTTCCC 389

Query: 391 LFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXXXXXVRAGTCLKSKNDVFGVKAMRRTA 450
           ++++   C+ WGCC    XXXXXXXXXXXXXXXXXXX        +K+    VKA++RT 
Sbjct: 390 IYEYGKYCYAWGCCPLEGXXXXXXXXXXXXXXXXXXXXXXXXXXMAKDSPLAVKALKRTL 449

Query: 451 AVAKPSWALGGGVSVGKSSA 467
           A    S+  G G    KSSA
Sbjct: 450 AKPNLSFLFGNG---KKSSA 458

BLAST of Carg26304 vs. Swiss-Prot
Match: sp|P25777|ORYB_ORYSJ (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 450.7 bits (1158), Expect = 2.0e-125
Identity = 243/420 (57.86%), Postives = 294/420 (70.00%), Query Frame = 0

Query: 33  TDEEVMALYDQWRAKH-GKLHNHLGPESQNRFHIFKDNLKYIDEINA---QNLPYRLGLN 92
           T+ E  A YD W A++ G   N LG E + RF +F DNLK++D  NA   +   +RLG+N
Sbjct: 44  TEAEARAAYDLWLAENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMN 103

Query: 93  VFADLTNEEYRSKYLGAKIAAGSRRNKTSSRYQPRLGDEVPDSIDWREKGAVAPIKDQGS 152
            FADLTNEE+R+ +LGAK+A  SR      RY+    +E+P+S+DWREKGAVAP+K+QG 
Sbjct: 104 RFADLTNEEFRATFLGAKVAERSR--AAGERYRHDGVEELPESVDWREKGAVAPVKNQGQ 163

Query: 153 CGSCWAFSTIAAVEGINQIVTGDLVALSEQELVDCDIS-YNEGCNGGLMDYAFEFIINNG 212
           CGSCWAFS ++ VE INQ+VTG+++ LSEQELV+C  +  N GCNGGLMD AF+FII NG
Sbjct: 164 CGSCWAFSAVSTVESINQLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNG 223

Query: 213 GIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDVPVNNEKALQKAAAKQVVAVAIEGGG 272
           GIDTE+DYPYK  D  C  +++NA+VVSID +EDVP N+EK+LQKA A Q V+VAIE GG
Sbjct: 224 GIDTEDDYPYKAVDGKCDINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGG 283

Query: 273 RSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYIRMQRNVA 332
           R FQLY SG+F+GRCGT LDHGV  VGYG++ G DYWIVRNSWG  WGESGY+RM+RN+ 
Sbjct: 284 REFQLYHSGVFSGRCGTSLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNIN 343

Query: 333 SPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPXXXXXXXXXXXXXDEYYDCPAAETCCCL 392
              G CGIAM  SYP K+G NPP         XXXXXXXXXX   D+ + CPA  TCCC 
Sbjct: 344 VTTGKCGIAMMASYPTKSGANPPXXXXXXXXXXXXXXXXXXXHVCDDNFSCPAGSTCCCA 403

Query: 393 FQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXXXXXVRAGTCLKSKNDVFGVKAMRRTAA 448
           F F N C VWGCC            XXXXXX      RAGTC  SKN    VKA++RT A
Sbjct: 404 FGFRNLCLVWGCCPVEGATCCKDHAXXXXXXYPVCNTRAGTCSASKNSPLSVKALKRTLA 461

BLAST of Carg26304 vs. Swiss-Prot
Match: sp|Q9LT78|RD21C_ARATH (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 6.3e-119
Identity = 221/400 (55.25%), Postives = 280/400 (70.00%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFF--LLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPE 60
           M T   S  + LL F  LL++L     ++    R + E   +Y++W  ++ K +N LG E
Sbjct: 1   MATSIKSITLALLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLG-E 60

Query: 61  SQNRFHIFKDNLKYIDEINA-QNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTS 120
            + RF IFKDNLK+++E ++  N  Y +GL  FADLTN+E+R+ YL +K+   +R     
Sbjct: 61  KERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMER-TRVPVKG 120

Query: 121 SRYQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSE 180
            +Y  ++GD +PD+IDWR KGAV P+KDQGSCGSCWAFS I AVEGINQI TG+L++LSE
Sbjct: 121 EKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSE 180

Query: 181 QELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYD-STCIQSKKNARVVSI 240
           QELVDCD SYN+GC GGLMDYAF+FII NGGIDTEEDYPY   D + C   KKN RVV+I
Sbjct: 181 QELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTI 240

Query: 241 DDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYG 300
           D YEDVP N+EK+L+KA A Q ++VAIE GGR+FQLY SG+FTG CGT LDHGV  VGYG
Sbjct: 241 DGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYG 300

Query: 301 SEGGVDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIK-TGLNPPNPGPT 360
           SEGG DYWIVRNSWG++WGESGY +++RN+    G CG+AM  SYP K +G NPP   P 
Sbjct: 301 SEGGQDYWIVRNSWGSNWGESGYFKLERNIKESSGKCGVAMMASYPTKSSGSNPPK--PP 360

Query: 361 PPSPXXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCF 396
            PSP             D+   CPA  TCCCL++++  C+
Sbjct: 361 APSP----------VVCDKSNTCPAKSTCCCLYEYNGKCY 386

BLAST of Carg26304 vs. TrEMBL
Match: tr|A0A1S3BV04|A0A1S3BV04_CUCME (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103493570 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 2.4e-192
Identity = 375/466 (80.47%), Postives = 407/466 (87.34%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ 60
           MGT++SS  + LLFFL + L +A PSSIIP+RTDEEVMALYDQWRA HGKLHN+LG E +
Sbjct: 1   MGTFQSSQTMALLFFLFIALSTASPSSIIPQRTDEEVMALYDQWRANHGKLHNNLGAEPE 60

Query: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120
           NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRS+YLG + AAGSRRN+TS+RY
Sbjct: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGRFAAGSRRNRTSNRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180
            PRLGDE+PDSIDWR KGAVAP+KDQGSCGSCWAFST+A+VEGINQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDELPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEGINQIVTGDLIALSEQEL 180

Query: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240
           VDCD SYNEGCNGGLMDYAFEFIINNGG+DTEEDYPY G+DS+CIQ KKNARVVSID YE
Sbjct: 181 VDCDKSYNEGCNGGLMDYAFEFIINNGGLDTEEDYPYYGFDSSCIQYKKNARVVSIDGYE 240

Query: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300
           DVPVNNEKALQKAA+KQVV+VAIEGGGRSFQLY+SGIFTGRCGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAASKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX 360
           VDYWIVRNSWGA+WGESGY+RMQRN+ SP GLCGIAMEPSYP K+G             X
Sbjct: 301 VDYWIVRNSWGANWGESGYVRMQRNIDSPTGLCGIAMEPSYPTKSG------PXXXXXXX 360

Query: 361 XXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXX 420
           XXXXXXXXX   DEYY CPAAETCCC+F+FS         XXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXSVCDEYYTCPAAETCCCIFEFSXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 467
           XXX       KSKND+FGVKAMRRTAA A+PSWA    V+VGKSSA
Sbjct: 421 XXXXXXXXXXKSKNDIFGVKAMRRTAAAARPSWA-HRDVTVGKSSA 459

BLAST of Carg26304 vs. TrEMBL
Match: tr|A0A0A0L3N7|A0A0A0L3N7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641600 PE=3 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 9.2e-192
Identity = 372/466 (79.83%), Postives = 407/466 (87.34%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQ 60
           MGT++SSPI+ LLFFL + L +A PSSIIP+RTD+EVMALYDQWRAKHGKLHN+LG E +
Sbjct: 1   MGTFQSSPIMALLFFLFIALSAASPSSIIPQRTDDEVMALYDQWRAKHGKLHNNLGAEPE 60

Query: 61  NRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRY 120
           NRFHIFKDNLK+IDEINAQNLPYRLGLNVFADLTNEEYRS+YLG K A+GSRRN+TS+RY
Sbjct: 61  NRFHIFKDNLKFIDEINAQNLPYRLGLNVFADLTNEEYRSRYLGGKFASGSRRNRTSNRY 120

Query: 121 QPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQEL 180
            PRLGD++PDSIDWR KGAVAP+KDQGSCGSCWAFST+A+VE INQIVTGDL+ALSEQEL
Sbjct: 121 LPRLGDDLPDSIDWRAKGAVAPVKDQGSCGSCWAFSTVASVEAINQIVTGDLIALSEQEL 180

Query: 181 VDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYE 240
           VDCD SYNEGCNGGLMDYAFEFII NGG+DTEEDYPY G+DS+CIQ KKNA+VV+ID YE
Sbjct: 181 VDCDRSYNEGCNGGLMDYAFEFIIENGGLDTEEDYPYYGFDSSCIQYKKNAKVVAIDGYE 240

Query: 241 DVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGG 300
           DVPVNNEKALQKA +KQVV+VAIEGGGRSFQLY+SGIFTGRCGTDLDHGVNVVGYGSEGG
Sbjct: 241 DVPVNNEKALQKAVSKQVVSVAIEGGGRSFQLYQSGIFTGRCGTDLDHGVNVVGYGSEGG 300

Query: 301 VDYWIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPX 360
           VDYWIVRNSWG SWGESGY++MQRN+ASP GLCGIAMEPSYP KTG             X
Sbjct: 301 VDYWIVRNSWGGSWGESGYVKMQRNIASPTGLCGIAMEPSYPTKTG------PXXXXXXX 360

Query: 361 XXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXX 420
           XXXXXXXXX   DEYY CPAAETCCC+FQFS         XXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXSVCDEYYTCPAAETCCCIFQFSXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 XXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKSSA 467
           XXX       KSKND+FGVKAMRRTAA A+PSWA    V+VGKSSA
Sbjct: 421 XXXXXXXXXXKSKNDIFGVKAMRRTAAAARPSWA-RRDVTVGKSSA 459

BLAST of Carg26304 vs. TrEMBL
Match: tr|M5XDT9|M5XDT9_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G431000 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 9.6e-157
Identity = 304/482 (63.07%), Postives = 361/482 (74.90%), Query Frame = 0

Query: 1   MGTYRSSPIVPLLFFLLVTLPSALPSSIIPE-------------RTDEEVMALYDQWRAK 60
           MG YRSS  + ++ F + TL SAL  SII               RTDEEVM++Y+ W AK
Sbjct: 1   MGPYRSSSTMAVVLFAIFTLSSALDMSIISYDDKNVVGESKSSWRTDEEVMSIYEGWLAK 60

Query: 61  HGKLHNHLGPESQNRFHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKI 120
           HGK +N LG E + RF IFKDNL+YID+ NA+NL Y+LGLN FADL+N+EYRS +LG K 
Sbjct: 61  HGKAYNGLG-EKERRFQIFKDNLRYIDDHNAKNLSYKLGLNRFADLSNDEYRSTFLGTKT 120

Query: 121 AAGSR----RNKTSSRYQPRLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEG 180
            A  R    RN  S RY PR+GD +PDS+DWR++G V PIKDQGSCGSCWAF+TI++VEG
Sbjct: 121 RAQKRRLSNRNTKSDRYAPRVGDSLPDSVDWRKEGVVNPIKDQGSCGSCWAFATISSVEG 180

Query: 181 INQIVTGDLVALSEQELVDCDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDST 240
           IN++VTGDL++LSEQELVDCD +YNEGCNGGLMDYAFEFII+NGGID+EEDYPYKGYD+T
Sbjct: 181 INKLVTGDLLSLSEQELVDCDKTYNEGCNGGLMDYAFEFIISNGGIDSEEDYPYKGYDAT 240

Query: 241 CIQSKKNARVVSIDDYEDVPVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCG 300
           C   +KNA+VVSIDDYEDVP  +EKAL+KA A Q +AVAIEGGGR FQLY SG+FTGRCG
Sbjct: 241 CDTYRKNAKVVSIDDYEDVPAYDEKALKKAVANQPIAVAIEGGGRDFQLYNSGVFTGRCG 300

Query: 301 TDLDHGVNVVGYGSEGGVDYWIVRNSWGASWGESGYIRMQRNVA-SPKGLCGIAMEPSYP 360
           T LDHGV VVGYG++ GVDYWIVRNSWG SWGE GYIRM+RN+  +  G+CGIAME SYP
Sbjct: 301 TSLDHGVTVVGYGTDKGVDYWIVRNSWGGSWGEEGYIRMERNLGNTAHGICGIAMEASYP 360

Query: 361 IKTGLNPPNPGP------TPPSPXXXXXXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFV 420
           +K+GLNPPNPG        PPS              D YY CP + TCCC+++++N CF 
Sbjct: 361 VKSGLNPPNPGXXXXXXVQPPS------------VCDNYYSCPESNTCCCIYEYANYCFA 420

Query: 421 WGCCXXXXXXXXXXXXXXXXXXXXXXXVRAGTCLKSKNDVFGVKAMRRTAAVAKPSWALG 459
           WGCC    XXXXXXXXXXXXXXXXXXX        SK++  GVKA++R    AKP WA+G
Sbjct: 421 WGCCPLEGXXXXXXXXXXXXXXXXXXXXXXXXXQLSKDNPLGVKALKRMP--AKPHWAIG 467

BLAST of Carg26304 vs. TrEMBL
Match: tr|A0A2R6Q0S0|A0A2R6Q0S0_ACTCH (Oryzain alpha chain like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc23770 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 3.7e-156
Identity = 302/451 (66.96%), Postives = 345/451 (76.50%), Query Frame = 0

Query: 12  LLFFLLVTLPSALPSSIIPE----RTDEEVMALYDQWRAKHGKLHNHLGPESQNRFHIFK 71
           LL F L  L SAL  SII E    RTD+EVMA+Y+ W  KHGK +N +G E + RF IFK
Sbjct: 13  LLLFSLFALSSALDMSIIGELSSSRTDDEVMAMYESWLVKHGKSYNAIG-EKEKRFQIFK 72

Query: 72  DNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRR----NKTSSRYQPR 131
           DNL++IDE NA++  Y++GLN FADLTN+EYRS YLGA+   GSRR     K S RY P 
Sbjct: 73  DNLRFIDEHNAESRTYKVGLNRFADLTNDEYRSMYLGAR--TGSRRRLSTQKRSDRYVPV 132

Query: 132 LGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQELVDC 191
            G+ +PDS+DWREKGAV  +KDQGSCGSCWAFSTIAAVEGINQIVTGDL++LSEQELVDC
Sbjct: 133 AGESLPDSVDWREKGAVVGVKDQGSCGSCWAFSTIAAVEGINQIVTGDLISLSEQELVDC 192

Query: 192 DISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDVP 251
           D SYNEGCNGGLMDYAFEFII NGGIDTEEDYPY   D  C Q +KNA+VV+IDDYEDVP
Sbjct: 193 DTSYNEGCNGGLMDYAFEFIIKNGGIDTEEDYPYNARDGRCDQYRKNAKVVTIDDYEDVP 252

Query: 252 VNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVDY 311
           VNNE+ALQKA A Q V+VAIE  G +FQ Y SG+FTG CGT LDHGV  VGYG+E  VDY
Sbjct: 253 VNNEQALQKAVANQPVSVAIEASGMAFQFYESGVFTGNCGTALDHGVTAVGYGTENSVDY 312

Query: 312 WIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPXXXX 371
           WIV+NSWG+SWGESGYIRM+RN  +  G CGIA+EPSYPIKT  NPPNPGP+PPSP    
Sbjct: 313 WIVKNSWGSSWGESGYIRMERNTGA-TGKCGIAVEPSYPIKTSQNPPNPGPSPPSP---- 372

Query: 372 XXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXXXXX 431
                    D+YY CP + TCCC++++   CF WGCCXXXXXXXXXXXXXXXXXX     
Sbjct: 373 --IKPPTVCDDYYTCPESSTCCCVYEYGKYCFAWGCCXXXXXXXXXXXXXXXXXXYPICN 432

Query: 432 VRAGTCLKSKNDVFGVKAMRRTAAVAKPSWA 455
           V AGTCL SK++  GVKAM+R    AKP WA
Sbjct: 433 VYAGTCLMSKDNPLGVKAMKRIQ--AKPQWA 451

BLAST of Carg26304 vs. TrEMBL
Match: tr|A5HIJ6|A5HIJ6_ACTDE (Cysteine protease Cp6 OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 3.7e-156
Identity = 302/451 (66.96%), Postives = 345/451 (76.50%), Query Frame = 0

Query: 12  LLFFLLVTLPSALPSSIIPE----RTDEEVMALYDQWRAKHGKLHNHLGPESQNRFHIFK 71
           LL F L  L SAL  SII E    RTD+EVMA+Y+ W  KHGK +N +G E + RF IFK
Sbjct: 13  LLLFSLFALSSALDMSIIGELSSSRTDDEVMAMYESWLVKHGKSYNAIG-EKEKRFQIFK 72

Query: 72  DNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRR----NKTSSRYQPR 131
           DNL++IDE NA++  Y++GLN FADLTN+EYRS YLGA+   GSRR     K S RY P 
Sbjct: 73  DNLRFIDEHNAESRTYKVGLNRFADLTNDEYRSMYLGAR--TGSRRRLSTQKRSDRYVPV 132

Query: 132 LGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQELVDC 191
            G+ +PDS+DWREKGAV  +KDQGSCGSCWAFSTIAAVEGINQIVTGDL++LSEQELVDC
Sbjct: 133 AGESLPDSVDWREKGAVVGVKDQGSCGSCWAFSTIAAVEGINQIVTGDLISLSEQELVDC 192

Query: 192 DISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDVP 251
           D SYNEGCNGGLMDYAFEFII NGGIDTEEDYPY   D  C Q +KNA+VV+IDDYEDVP
Sbjct: 193 DTSYNEGCNGGLMDYAFEFIIKNGGIDTEEDYPYNARDGRCDQYRKNAKVVTIDDYEDVP 252

Query: 252 VNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVDY 311
           VNNE+ALQKA A Q V+VAIE  G +FQ Y SG+FTG CGT LDHGV  VGYG+E  VDY
Sbjct: 253 VNNEQALQKAVANQPVSVAIEASGMAFQFYESGVFTGNCGTALDHGVTAVGYGTENSVDY 312

Query: 312 WIVRNSWGASWGESGYIRMQRNVASPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPXXXX 371
           WIV+NSWG+SWGESGYIRM+RN  +  G CGIA+EPSYPIKT  NPPNPGP+PPSP    
Sbjct: 313 WIVKNSWGSSWGESGYIRMERNTGA-TGKCGIAVEPSYPIKTSQNPPNPGPSPPSP---- 372

Query: 372 XXXXXXXXXDEYYDCPAAETCCCLFQFSNTCFVWGCCXXXXXXXXXXXXXXXXXXXXXXX 431
                    D+YY CP + TCCC++++   CF WGCCXXXXXXXXXXXXXXXXXX     
Sbjct: 373 --IKPPTVCDDYYTCPESSTCCCVYEYGKYCFAWGCCXXXXXXXXXXXXXXXXXXYPICN 432

Query: 432 VRAGTCLKSKNDVFGVKAMRRTAAVAKPSWA 455
           V AGTCL SK++  GVKAM+R    AKP WA
Sbjct: 433 VYAGTCLMSKDNPLGVKAMKRIQ--AKPQWA 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022936812.14.4e-24699.36low-temperature-induced cysteine proteinase-like [Cucurbita moschata][more]
XP_022977015.19.2e-24498.50low-temperature-induced cysteine proteinase-like [Cucurbita maxima][more]
XP_023534814.12.7e-24396.82low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo][more]
XP_008452589.23.7e-19280.47PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo][more]
XP_004141271.21.4e-19179.83PREDICTED: low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN552... [more]
Match NameE-valueIdentityDescription
AT5G43060.19.7e-14759.28Granulin repeat cysteine protease family protein[more]
AT1G47128.12.8e-14660.60Granulin repeat cysteine protease family protein[more]
AT3G19390.13.5e-12055.25Granulin repeat cysteine protease family protein[more]
AT4G36880.11.5e-11559.66cysteine proteinase1[more]
AT4G23520.12.7e-10453.31Cysteine proteinases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9FMH8|RD21B_ARATH1.8e-14559.28Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S... [more]
sp|P43297|RD21A_ARATH5.1e-14560.60Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1[more]
sp|P25776|ORYA_ORYSJ1.5e-13362.50Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=... [more]
sp|P25777|ORYB_ORYSJ2.0e-12557.86Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1... [more]
sp|Q9LT78|RD21C_ARATH6.3e-11955.25Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BV04|A0A1S3BV04_CUCME2.4e-19280.47low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC1... [more]
tr|A0A0A0L3N7|A0A0A0L3N7_CUCSA9.2e-19279.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641600 PE=3 SV=1[more]
tr|M5XDT9|M5XDT9_PRUPE9.6e-15763.07Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G431000 PE=3 SV=1[more]
tr|A0A2R6Q0S0|A0A2R6Q0S0_ACTCH3.7e-15666.96Oryzain alpha chain like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY... [more]
tr|A5HIJ6|A5HIJ6_ACTDE3.7e-15666.96Cysteine protease Cp6 OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR038765Papain_like_cys_pep_sf
IPR039417Peptidase_C1A_papain-like
IPR025661Pept_asp_AS
IPR025660Pept_his_AS
IPR000169Pept_cys_AS
IPR013128Peptidase_C1A
IPR037277Granulin_sf
IPR000118Granulin
IPR013201Prot_inhib_I29
IPR000668Peptidase_C1A_C
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26304-RACarg26304-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 288..298
score: 56.8
coord: 146..161
score: 65.92
coord: 303..309
score: 73.06
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 128..343
e-value: 1.3E-121
score: 420.0
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 128..343
e-value: 1.9E-82
score: 276.5
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 41..98
e-value: 3.4E-17
score: 73.1
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 41..98
e-value: 3.5E-11
score: 43.3
IPR000118GranulinSMARTSM00277GRAN_2coord: 372..429
e-value: 3.7E-29
score: 112.9
IPR000118GranulinPFAMPF00396Granulincoord: 383..430
e-value: 1.5E-10
score: 41.2
IPR037277Granulin superfamilyGENE3DG3DSA:3.10.20.500coord: 369..432
e-value: 7.7E-13
score: 50.5
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 28..344
e-value: 1.1E-113
score: 382.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 345..364
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 344..364
NoneNo IPR availablePANTHERPTHR12411:SF352SUBFAMILY NOT NAMEDcoord: 29..448
NoneNo IPR availableSUPERFAMILYSSF57277Granulin repeatcoord: 369..403
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 29..448
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 146..157
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 286..296
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 303..322
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 129..342
e-value: 2.68927E-99
score: 298.384
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILYSSF54001Cysteine proteinasescoord: 33..343

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg26304Carg10617Silver-seed gourdcarcarB606