Carg27195-RA (mRNA) Silver-seed gourd

NameCarg27195-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPyrophosphate-energized vacuolar membrane proton pump
LocationCucurbita_argyrosperma_scaffold_486 : 58183 .. 62916 (-)
Sequence length2881
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTCCTATCCCCATTTTTCTCTCTCTTCCATTTGGTTTGGGAGATGAATATAAATGTGAGTTTCCATTTCCCTCAACGCATCTCAGCCTGAAATCGCGTCCTTCCATTCACTCACTCTGCCACCACACCCCACCAAACCCTCGTTTTCTTCTTCCTTTTATCCTTCGTTTCGTGGGGTTCGTTTCCCTGTGTGTGTTGGTAATTCTTTCGCCATGAGTGTTACCATTCTTCCAGATCTCGGGACTGAGATTTTCATTCCGGTATGTGCCGTTGTCGGAATCGTCTTCTCTTTGGTGCAGTGGTATTATGTTTCGCAGGTTAAGCTCTCGCCTGGCCGAGATGCCGCTCACAACAACAGCGCCGGTTCTAAGAATGGCTACTCCGACTACCTCATCGAGGAGGAAGAGGGCGTCAACGACCATAACGTTGTGATTAAGTGTGCCGAAATTCAGAGTGCCATTTCTGAGGGTATATCGTTTTCTCGTTCTTGTTCGTTTCAGATTTGGTTTCTGCTGTCTTCTTTTTTGCCTTATTAATTCTTGGATCTGGTGCTGGATTCTCAGATTTCTTGTTCTTACATTTTGGTTGTCTCGTTTATGGATTTTTTTTTTTTGTTTTTTTTTCCTCTTATGAATGCTCTGTAGAGTTTATCTTGATTTCACTTCTCGGTTCTTTCCTGGCTCTTGATTTTTGGAGCGGTTTTTGTTTTTGTTAGATGGTAACGTACACTTTGGAAGTTGCGCGTTGTGTTGTGTTAGTTTTGTTGCGCGATTATCGTGTTGTGGTATACGTGTGGATGTTACATTGGTCCGATCTTTCCATGCATTTATGGGCCGTGCTTGTTGTCTTTAAGCTAATACGCTACGTACCCTGTGTTCCGTGGGCATCCTTCCCTGCTTGACCTTGACAGTTAGGTTCACTGCACTGGTCTTAACACATTACCATAAATAGCTTACGGAAGCGTTAATTACGAATTATTTATTTCTTTTGATTGATTATATCTTGTCTGTTCACATGGAGTTTATTGGAGTGTCTGTATACATGAATTTATGAAGAGATCTAGGAACTTATGATCGATTAGTTTCAGTTTATTAAAAACAGAGGGCCGGACTGTCAGAACCTTTGGAACTTAATTCTCTTATATGGGTTTGTAGTGTTTCTTTTTTGGTTAAGAGGATAGAGTTGCATCACCACATCTGGAGTTTAATCAGCATTTCTTCCAAGTTTATGGGATTCCTGTTTCTTTGCGTCTGGTTCCATGTTAGTGTTTTTTCGTTCTGTATGGTATTTATTTAATTGTTAGTTTTGTGTATGCCATGCAAAGAATCATATTCATATCCATGATTTTTTTTGGATGAATCATTGGAAATATTTTTATGAGTTCCTTTTGTTGAAGTGTTCTACGTTAGTGCCTTCTGGAAGTTTTTTTTGTCACTCTGTTTTTTGTTGCTTCCTTTTATTGAAGATGCCATCTTCTAGAAATTGTAACTCTTAAGCCAATATTTATATATACTTTTTCTTGCTATGAAAAGGAAGTGAAAAGTATTGTTGTGTCCTCTCATTAGTAAAGCATGTAAGAATATGTGTCGTTTAAGATGACAAAACCACTTTTACTAATTTTATCGGAAAGTTTATTCTTTTGAATCATATGGTTTTATGCTTTCTCCTTGCGTTGTTTTAGAGAAGTTTCTAACCTTTTTTACTCTTTGATGTATTAGGAGCAACGTCCTTCCTTTTCACGGAGTACAAGTACGTTGGCATCTTTATGGTGCTGTTTGCAGTCTTGATTTTCGTATTCCTTGGCTCAGTTGAGAGTTTTAGTACCAAGCCTCAACCGTGCACATATGACAAAACAAGGACTTGTAAGCCAGCTTTGGCGACTGCTATATTTAGCACTGTATCATTTTTACTTGGTGCAGTTACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATACGCAAATGCCAGAACCACCTTGGAGGCAAGAAAGGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTTCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACCTCTTCAAATTGTACTATGGTGATGATTGGGGTGGTCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGTTCTTCAATGGCTCTCTTTGGAAGAGTTGGTGGTGGTATCTACACCAAAGCTGCCGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATCCCCGAGGATGATCCCAGAAACCCAGCTGTAATTTTCATCACTCTAAATATATTTTTTTTGTAACTTTTGTGCTGAACTTCTATCTCTTAATCGAATCACTCATTGTTTTGGCAGGTGATTGCTGACAATGTTGGTGACAATGTTGGGGATATAGCCGGTATGGGATCTGATCTGTTTGGTTCATATGCTGAATCTTCATGTGCTGCTCTCGTTGTTGCTTCTATCTCTTCTTTTGGAAACAACCATGAATTGACACCAATGCTCTACCCTCTCATCGTTAGTTCTATGGGTATTCTTGTTTGCCTGATCACCACCTTATTTGCAACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTTATAATTTCCACTGTTCTAATGACCTTTGGAATTGCAATTGTTACTTGGTTGTCTGTTCCATCATCATTCACCATTTTCAACTTTGGAACTCAAAAAGTTGTTACGAACTGGTTAGTTCTTGAATTATTTTCTTACAATTGCTTTTCATTGTCACTAGTGGTTTGATTCACGGTCGAGTATTTGGCTTTCAGGAAACTGTTCTTGTGCGTTGCTGTGGGTCTCTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGGTAATTGATTTATTTGCATTTACCTATTCACTCAGTAATTGATTTGTTATATAAAGATGAAAATCTGATCACTTATTTTTCTGATTTTATTCAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATTTTTGGGTTGGCGTTGGGATACAAATCTGTCATTATCCCTATTTTTGCAATTGCGGTCAGTATTTTTGTGAGTTTCACCTTTGCTGCCATGTACGGCATTGCTGTAGCTGCCCTTGGAATGCTGAGTACCATAGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACTGATGCCCTCGATGCTGCTGGAAACACCACTGCAGCCATTGGAAAGGTATTTTTTTGGATATTCTTTTAACCCAAATGTAATGCCTGGATCGGTTCTTTATTTACCTTGGTCTTGGTCTTGGTCTTCTGTATATTGTCTCAATAGAATGTTTTTCCAGTTTCCCACCAAATCCTTCCAATGAACTTCTGTCAGAACTTCACACTTTATTTGCCTTTTCCAGGGTTTTGCCATTGGTTCAGCTGCCCTTGTGTCCCTTGCTCTTTTCGGTGCCTTTGTCAGCCGTGCCGGTGTCACTGCTGTTGACGTCTTGACTCCCAAAGTTTTTATTGGTTTGATTGTGGGTGCCATGCTCCCCTACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGTAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAGTTCAACACTATCCCAGGTCTCATGGAGGGTACTGCCAAGCCCGACTACGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCTCTTGTCATGTTGACACCACTAATTGTTGGTATCCTTTTTGGAGTTGAAACTCTTTCTGGTGTCCTTGCTGGATCCCTTGTTTCTGGTGTGCAGGTATGTGTCGGGTTCATTCTTGGAACTACTTGTTAAACATCAAAGTTTTTCACTAAACGATGTTCTTGTTTTGCACAACCGTAGATTGCCATTTCTGCATCTAACACCGGAGGTGCCTGGGATAACGCTAAGAAGTACATCGAGGTATATGCCAAATCCAATAATCATGTATCCAATGTTGTAATGATTTTCCGGTATTTGATCAACATCTGATTGTCATGGACAGGCTGGTGCCTCTGAGCACGCAAGAACCCTTGGCCCGAAAGGATCAGACCCACACAAAGCCGCTGTTATCGGTGACACAATCGGAGACCCGCTGAAGGATACATCCGGACCATCTTTGAACATTCTGATCAAGTTGATGGCTGTCGAGTCCCTTGTGTTTGCTCCTTTCTTTGCCAGTCACGGTGGTCTTCTCTTCAAGATCTTCTGAAAAGAACAACACGAGTAATGGGGGAATGGCTGGGGCATTGCATTATCTCTGGCCCTCCTCCTCCATTGACATAACTACCGCGCCTCTTCTCCGAAATTTTCATTGCTTTTTTAGACTCCATGTTATATGCTCCTCAAGTCTGTAGCTTTTGATTCCCTTTGAGAAACGTGTAGTTTCAAATGGACGAGCGTTTTATCTTCTCATCGGTTGATGATGATGAAAGAAAAAAAAGAAGATATTTGGTTAAATCTTGTGAAATGAGACTGCGACCTTATTCTCTTTTTCCCAATTGAGAACAGGCTATTATTGTTGTTCCCACACACTGTCTCTCTGTTCTTGAATGCGACTTCCATTTTCATAT

mRNA sequence

TATTCCTATCCCCATTTTTCTCTCTCTTCCATTTGGTTTGGGAGATGAATATAAATGTGAGTTTCCATTTCCCTCAACGCATCTCAGCCTGAAATCGCGTCCTTCCATTCACTCACTCTGCCACCACACCCCACCAAACCCTCGTTTTCTTCTTCCTTTTATCCTTCGTTTCGTGGGGTTCGTTTCCCTGTGTGTGTTGGTAATTCTTTCGCCATGAGTGTTACCATTCTTCCAGATCTCGGGACTGAGATTTTCATTCCGGTATGTGCCGTTGTCGGAATCGTCTTCTCTTTGGTGCAGTGGTATTATGTTTCGCAGGTTAAGCTCTCGCCTGGCCGAGATGCCGCTCACAACAACAGCGCCGGTTCTAAGAATGGCTACTCCGACTACCTCATCGAGGAGGAAGAGGGCGTCAACGACCATAACGTTGTGATTAAGTGTGCCGAAATTCAGAGTGCCATTTCTGAGGGAGCAACGTCCTTCCTTTTCACGGAGTACAAGTACGTTGGCATCTTTATGGTGCTGTTTGCAGTCTTGATTTTCGTATTCCTTGGCTCAGTTGAGAGTTTTAGTACCAAGCCTCAACCGTGCACATATGACAAAACAAGGACTTGTAAGCCAGCTTTGGCGACTGCTATATTTAGCACTGTATCATTTTTACTTGGTGCAGTTACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATACGCAAATGCCAGAACCACCTTGGAGGCAAGAAAGGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTTCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACCTCTTCAAATTGTACTATGGTGATGATTGGGGTGGTCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGTTCTTCAATGGCTCTCTTTGGAAGAGTTGGTGGTGGTATCTACACCAAAGCTGCCGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATCCCCGAGGATGATCCCAGAAACCCAGCTGTGATTGCTGACAATGTTGGTGACAATGTTGGGGATATAGCCGGTATGGGATCTGATCTGTTTGGTTCATATGCTGAATCTTCATGTGCTGCTCTCGTTGTTGCTTCTATCTCTTCTTTTGGAAACAACCATGAATTGACACCAATGCTCTACCCTCTCATCGTTAGTTCTATGGGTATTCTTGTTTGCCTGATCACCACCTTATTTGCAACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTTATAATTTCCACTGTTCTAATGACCTTTGGAATTGCAATTGTTACTTGGTTGTCTGTTCCATCATCATTCACCATTTTCAACTTTGGAACTCAAAAAGTTGTTACGAACTGGAAACTGTTCTTGTGCGTTGCTGTGGGTCTCTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATTTTTGGGTTGGCGTTGGGATACAAATCTGTCATTATCCCTATTTTTGCAATTGCGGTCAGTATTTTTGTGAGTTTCACCTTTGCTGCCATGTACGGCATTGCTGTAGCTGCCCTTGGAATGCTGAGTACCATAGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACTGATGCCCTCGATGCTGCTGGAAACACCACTGCAGCCATTGGAAAGGGTTTTGCCATTGGTTCAGCTGCCCTTGTGTCCCTTGCTCTTTTCGGTGCCTTTGTCAGCCGTGCCGGTGTCACTGCTGTTGACGTCTTGACTCCCAAAGTTTTTATTGGTTTGATTGTGGGTGCCATGCTCCCCTACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGTAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAGTTCAACACTATCCCAGGTCTCATGGAGGGTACTGCCAAGCCCGACTACGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCTCTTGTCATGTTGACACCACTAATTGTTGGTATCCTTTTTGGAGTTGAAACTCTTTCTGGTGTCCTTGCTGGATCCCTTGTTTCTGGTGTGCAGATTGCCATTTCTGCATCTAACACCGGAGGTGCCTGGGATAACGCTAAGAAGTACATCGAGGCTGGTGCCTCTGAGCACGCAAGAACCCTTGGCCCGAAAGGATCAGACCCACACAAAGCCGCTGTTATCGGTGACACAATCGGAGACCCGCTGAAGGATACATCCGGACCATCTTTGAACATTCTGATCAAGTTGATGGCTGTCGAGTCCCTTGTGTTTGCTCCTTTCTTTGCCAGTCACGGTGGTCTTCTCTTCAAGATCTTCTGAAAAGAACAACACGAGTAATGGGGGAATGGCTGGGGCATTGCATTATCTCTGGCCCTCCTCCTCCATTGACATAACTACCGCGCCTCTTCTCCGAAATTTTCATTGCTTTTTTAGACTCCATGTTATATGCTCCTCAAGTCTGTAGCTTTTGATTCCCTTTGAGAAACGTGTAGTTTCAAATGGACGAGCGTTTTATCTTCTCATCGGTTGATGATGATGAAAGAAAAAAAAGAAGATATTTGGTTAAATCTTGTGAAATGAGACTGCGACCTTATTCTCTTTTTCCCAATTGAGAACAGGCTATTATTGTTGTTCCCACACACTGTCTCTCTGTTCTTGAATGCGACTTCCATTTTCATAT

Coding sequence (CDS)

ATGAGTGTTACCATTCTTCCAGATCTCGGGACTGAGATTTTCATTCCGGTATGTGCCGTTGTCGGAATCGTCTTCTCTTTGGTGCAGTGGTATTATGTTTCGCAGGTTAAGCTCTCGCCTGGCCGAGATGCCGCTCACAACAACAGCGCCGGTTCTAAGAATGGCTACTCCGACTACCTCATCGAGGAGGAAGAGGGCGTCAACGACCATAACGTTGTGATTAAGTGTGCCGAAATTCAGAGTGCCATTTCTGAGGGAGCAACGTCCTTCCTTTTCACGGAGTACAAGTACGTTGGCATCTTTATGGTGCTGTTTGCAGTCTTGATTTTCGTATTCCTTGGCTCAGTTGAGAGTTTTAGTACCAAGCCTCAACCGTGCACATATGACAAAACAAGGACTTGTAAGCCAGCTTTGGCGACTGCTATATTTAGCACTGTATCATTTTTACTTGGTGCAGTTACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATACGCAAATGCCAGAACCACCTTGGAGGCAAGAAAGGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTTCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACCTCTTCAAATTGTACTATGGTGATGATTGGGGTGGTCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGTTCTTCAATGGCTCTCTTTGGAAGAGTTGGTGGTGGTATCTACACCAAAGCTGCCGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATCCCCGAGGATGATCCCAGAAACCCAGCTGTGATTGCTGACAATGTTGGTGACAATGTTGGGGATATAGCCGGTATGGGATCTGATCTGTTTGGTTCATATGCTGAATCTTCATGTGCTGCTCTCGTTGTTGCTTCTATCTCTTCTTTTGGAAACAACCATGAATTGACACCAATGCTCTACCCTCTCATCGTTAGTTCTATGGGTATTCTTGTTTGCCTGATCACCACCTTATTTGCAACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTTATAATTTCCACTGTTCTAATGACCTTTGGAATTGCAATTGTTACTTGGTTGTCTGTTCCATCATCATTCACCATTTTCAACTTTGGAACTCAAAAAGTTGTTACGAACTGGAAACTGTTCTTGTGCGTTGCTGTGGGTCTCTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATTTTTGGGTTGGCGTTGGGATACAAATCTGTCATTATCCCTATTTTTGCAATTGCGGTCAGTATTTTTGTGAGTTTCACCTTTGCTGCCATGTACGGCATTGCTGTAGCTGCCCTTGGAATGCTGAGTACCATAGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCTGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACTGATGCCCTCGATGCTGCTGGAAACACCACTGCAGCCATTGGAAAGGGTTTTGCCATTGGTTCAGCTGCCCTTGTGTCCCTTGCTCTTTTCGGTGCCTTTGTCAGCCGTGCCGGTGTCACTGCTGTTGACGTCTTGACTCCCAAAGTTTTTATTGGTTTGATTGTGGGTGCCATGCTCCCCTACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGTAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAGTTCAACACTATCCCAGGTCTCATGGAGGGTACTGCCAAGCCCGACTACGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCTCTTGTCATGTTGACACCACTAATTGTTGGTATCCTTTTTGGAGTTGAAACTCTTTCTGGTGTCCTTGCTGGATCCCTTGTTTCTGGTGTGCAGATTGCCATTTCTGCATCTAACACCGGAGGTGCCTGGGATAACGCTAAGAAGTACATCGAGGCTGGTGCCTCTGAGCACGCAAGAACCCTTGGCCCGAAAGGATCAGACCCACACAAAGCCGCTGTTATCGGTGACACAATCGGAGACCCGCTGAAGGATACATCCGGACCATCTTTGAACATTCTGATCAAGTTGATGGCTGTCGAGTCCCTTGTGTTTGCTCCTTTCTTTGCCAGTCACGGTGGTCTTCTCTTCAAGATCTTCTGA

Protein sequence

MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYLIEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF
BLAST of Carg27195-RA vs. NCBI nr
Match: XP_022929683.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata])

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 768/768 (100.00%), Postives = 768/768 (100.00%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 768

BLAST of Carg27195-RA vs. NCBI nr
Match: XP_023002108.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita maxima])

HSP 1 Score: 1426.0 bits (3690), Expect = 0.0e+00
Identity = 766/768 (99.74%), Postives = 768/768 (100.00%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           MSVTILPDLGTEIFIPVCAV+GI+FSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIIFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 768

BLAST of Carg27195-RA vs. NCBI nr
Match: XP_023529940.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 766/768 (99.74%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQ AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQCAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLI+SSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 768

BLAST of Carg27195-RA vs. NCBI nr
Match: BAA33149.1 (proton-translocating inorganic pyrophosphatase [Cucurbita moschata])

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 765/768 (99.61%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           MSVTILPDLGTEIFIPVCAV+GIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASIS FGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISPFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGAS+HARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASKHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 768

BLAST of Carg27195-RA vs. NCBI nr
Match: XP_023515840.1 (pyrophosphate-energized vacuolar membrane proton pump [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1388.6 bits (3593), Expect = 0.0e+00
Identity = 742/765 (96.99%), Postives = 759/765 (99.22%), Query Frame = 0

Query: 3   VTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYLIE 62
           VTILPDLGT+IFIPVCAVVGIVFSLVQWYYVSQVKLSPGRD+ HNNSA +KNGYSDYLIE
Sbjct: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIE 63

Query: 63  EEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTK 122
           EEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVE FST+
Sbjct: 64  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTE 123

Query: 123 PQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 182
           PQ CTYDKT+ CKPALATAIFST+SFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG
Sbjct: 124 PQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 183

Query: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242
           KAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALF
Sbjct: 184 KAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALF 243

Query: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYA 302
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYA
Sbjct: 244 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYA 303

Query: 303 ESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPA 362
           ESSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCLITTLFATDFFEIKAVKEIEPA
Sbjct: 304 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPA 363

Query: 363 LKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGF 422
           LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLIIGF
Sbjct: 364 LKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGF 423

Query: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 482
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY
Sbjct: 424 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 483

Query: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542
           GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 484 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 543

Query: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602
           KGFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 544 KGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 603

Query: 603 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF
Sbjct: 604 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 663

Query: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 722
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV
Sbjct: 664 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 723

Query: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLF++
Sbjct: 724 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768

BLAST of Carg27195-RA vs. TAIR10
Match: AT1G15690.1 (Inorganic H pyrophosphatase family protein)

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 673/765 (87.97%), Postives = 719/765 (93.99%), Query Frame = 0

Query: 5   ILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGS-KNGYSDYLIEE 64
           +LP+L TEI +P+CAV+GI FSL QWY VS+VKL+    A+ +  A + KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKP 124
           EEGVND +VV KCAEIQ+AISEGATSFLFTEYKYVG+FM+ FA +IFVFLGSVE FST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 184
           +PCTYD TRTCKPALATA FST++F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADN XXXXXXXXGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNXXXXXXXXXGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 364
           +SCAALVVASISSFG NH+ T M YPL++SSMGILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGFV 424
           K QLIISTV+MT GIAIV+W+ +P+SFTIFNFGTQKVV NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGG+LFK F
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFKYF 770

BLAST of Carg27195-RA vs. TAIR10
Match: AT1G16780.1 (Inorganic H pyrophosphatase family protein)

HSP 1 Score: 406.8 bits (1044), Expect = 3.1e-113
Identity = 283/726 (38.98%), Postives = 404/726 (55.65%), Query Frame = 0

Query: 78  EIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQPCTYDKTRTCKPA 137
           +I  AI +GA  FL T+Y   G    +  +L FV L      +  PQ       RT    
Sbjct: 150 QISDAIRDGAEGFLRTQY---GTISKMAFLLAFVILCIYLFRNLTPQQEASGLGRT---- 209

Query: 138 LATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGF 197
             +A  +  +FLLGA+ S ++G++GM ++  AN R +  AR+   +A   A R+G     
Sbjct: 210 -MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSAL 269

Query: 198 LLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGGIYT 257
           ++    ++ + I  + F ++   D  G  +       + GYG G S +ALF ++GGGIYT
Sbjct: 270 VVVGMAVIGIAILYSTFYVWLDVDSPGSMKVTDLPLLLVGYGFGASFVALFAQLGGGIYT 329

Query: 258 KAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYAESSCAALVV 317
           K ADVGADLVGKVE  IPEDDPRNPAVIAD V          G+DLF S A    +A+++
Sbjct: 330 KGADVGADLVGKVEHGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMIL 389

Query: 318 ASISSFGNNHELTP--MLYPLIVSSMGILVCL--ITTLFATDFFEIKA-VKEIEPALKK- 377
               +     E     +L+PL+V S  +++    I ++  T    +K+ V++    L+K 
Sbjct: 390 GGTMAQKCKIEDPSGFILFPLVVHSFDLVISSIGILSIKGTRNASVKSPVEDPMVVLQKG 449

Query: 378 -QLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNW-KLFLCVAVGLWAGLIIGFV 437
             L I   ++TFG A   WL            T++  + W   F+C  VG+    +  ++
Sbjct: 450 YSLTIILAVLTFG-ASTRWL----------LYTEQAPSAWLNFFMCGLVGIITAYVFVWI 509

Query: 438 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF----- 497
           + YYT   Y PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  +F       
Sbjct: 510 SRYYTDYKYEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLVISVAIISAFWLGNTSG 569

Query: 498 ---------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTD 557
                      ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE TD
Sbjct: 570 LIDEKGNPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITD 629

Query: 558 ALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTA------VDVLTPKVFIGLIV 617
            LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  P+VFIG ++
Sbjct: 630 VLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFIGGLL 689

Query: 618 GAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKE 677
           GAML + FSA    +VG  A ++V EVRRQF   PG+M+   KPDY  CV I   ++++E
Sbjct: 690 GAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMDYKEKPDYGRCVAIVASSALRE 749

Query: 678 MIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNTGGA 737
           MI PGAL +++P+ VG +F            G + ++ +L  + V G+ +A+  +  GGA
Sbjct: 750 MIKPGALAIISPIAVGFVFRILGYYTGQPLLGAKVVAAMLMFATVCGILMALFLNTAGGA 809

Query: 738 WDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESL 758
           WDNAKKYIE GA      LG KGSD HKAAV GDT+GDP KDT+GPS+++LIK++A  +L
Sbjct: 810 WDNAKKYIETGA------LGGKGSDSHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITL 850

BLAST of Carg27195-RA vs. TAIR10
Match: AT1G78920.1 (vacuolar H+-pyrophosphatase 2)

HSP 1 Score: 405.6 bits (1041), Expect = 6.8e-113
Identity = 285/729 (39.09%), Postives = 406/729 (55.69%), Query Frame = 0

Query: 78  EIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQPCTYDKTRTCKPA 137
           EI  AI +GA  F  T+Y  +    +L A   FV L      S  PQ       R     
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLA---FVILCIYLFRSLTPQQEAAGLGRA---- 160

Query: 138 LATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGF 197
             +A  +  +FLLGA+ S ++G++GM ++  AN R +  AR+   +A   A R+G     
Sbjct: 161 -MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSAL 220

Query: 198 LLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGGIYT 257
           ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGGIYT
Sbjct: 221 VVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIYT 280

Query: 258 KAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYAESSCAALVV 317
           K ADVGADLVGKVE+ IPEDDPRNPAVIAD V          G+DLF S A    +A+++
Sbjct: 281 KGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMIL 340

Query: 318 ASISSFGNNHELTP--MLYPLIVSSMGILVCL--ITTLFATDFFEIKA-VKEIEPALKK- 377
               +     E     +L+PL+V S  +++    I ++  T    +K+ V++    L+K 
Sbjct: 341 GGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGILSIKGTRDASVKSPVEDPMAVLQKG 400

Query: 378 -QLIISTVLMTFGIAIVTWL----SVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 437
             L I   ++TFG A   WL      PS++  FNF            LC  VG+    I 
Sbjct: 401 YSLTIILAVITFG-ASTRWLLYTEQAPSAW--FNFA-----------LCGLVGIITAYIF 460

Query: 438 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF-- 497
            ++++YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  ++    
Sbjct: 461 VWISKYYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGN 520

Query: 498 ------------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRE 557
                         ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE
Sbjct: 521 TSGLVDENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVRE 580

Query: 558 RTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTA------VDVLTPKVFIG 617
            TD LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  P+VF+G
Sbjct: 581 ITDLLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVG 640

Query: 618 LIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDAS 677
            ++GAML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A+
Sbjct: 641 GLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAA 700

Query: 678 IKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNT 737
           ++EMI PGAL + +P++VG++F            G + ++ +L  + V G+ +A+  +  
Sbjct: 701 LREMIKPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTA 760

Query: 738 GGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAV 758
           GGAWDNAKKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A 
Sbjct: 761 GGAWDNAKKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLAT 801

BLAST of Carg27195-RA vs. Swiss-Prot
Match: sp|P21616|AVP_VIGRR (Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radiata OX=3916 PE=1 SV=4)

HSP 1 Score: 1317.4 bits (3408), Expect = 0.0e+00
Identity = 705/768 (91.80%), Postives = 740/768 (96.35%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           M   ILPDLGTEI IPVCAV+GI F+L QW  VS+VKLS  RDA+ N  A +KNGY+DYL
Sbjct: 1   MGAAILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPN--AAAKNGYNDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEG+NDHNVV+KCAEIQ+AISEGATSFLFTEYKYVGIFMV FA+LIF+FLGSVE FS
Sbjct: 61  IEEEEGINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           T PQ C+YDKT+TCKPALATAIFSTVSFLLG VTS+VSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TSPQACSYDKTKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVL+IAINLFK+YYGDDWGGLFE+ITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLYIAINLFKIYYGDDWGGLFEAITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFG NHELT MLYPLIVSS+GILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGLNHELTAMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALKKQL+ISTVLMT G+A+V+++++P+SFTIFNFG QK V +W+LFLCVAVGLWAGLII
Sbjct: 361 PALKKQLVISTVLMTIGVAVVSFVALPTSFTIFNFGVQKDVKSWQLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRA +T VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRASITTVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPL+VGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLVVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHAR+LGPKGSD HKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARSLGPKGSDCHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKIF 766

BLAST of Carg27195-RA vs. Swiss-Prot
Match: sp|P31414|AVP1_ARATH (Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana OX=3702 GN=AVP1 PE=1 SV=1)

HSP 1 Score: 1268.8 bits (3282), Expect = 0.0e+00
Identity = 673/765 (87.97%), Postives = 719/765 (93.99%), Query Frame = 0

Query: 5   ILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGS-KNGYSDYLIEE 64
           +LP+L TEI +P+CAV+GI FSL QWY VS+VKL+    A+ +  A + KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKP 124
           EEGVND +VV KCAEIQ+AISEGATSFLFTEYKYVG+FM+ FA +IFVFLGSVE FST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 184
           +PCTYD TRTCKPALATA FST++F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADN XXXXXXXXGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNXXXXXXXXXGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPAL 364
           +SCAALVVASISSFG NH+ T M YPL++SSMGILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGFV 424
           K QLIISTV+MT GIAIV+W+ +P+SFTIFNFGTQKVV NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGG+LFK F
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFKYF 770

BLAST of Carg27195-RA vs. Swiss-Prot
Match: sp|Q06572|AVP_HORVU (Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513 PE=2 SV=2)

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 669/764 (87.57%), Postives = 722/764 (94.50%), Query Frame = 0

Query: 3   VTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYLIE 62
           + IL +LGTEI IPVC V+GIVF++ QW+ VS+VK++PG  +A   +AG+KNGY DYLIE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASA---AAGAKNGYGDYLIE 60

Query: 63  EEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTK 122
           EEEG+NDHNVV+KCAEIQ+AISEGATSFLFT Y+YVG+FMV+FA +IF+FLGS+E FSTK
Sbjct: 61  EEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTK 120

Query: 123 PQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 182
            QPCTY K  TCKPAL TA+FST SFLLGA+TS+VSGFLGMKIATYANARTTLEARKGVG
Sbjct: 121 GQPCTYSK-GTCKPALYTALFSTASFLLGAITSLVSGFLGMKIATYANARTTLEARKGVG 180

Query: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242
           KAFITAFRSGAVMGFLL+++GL+VL+I IN+FK+YYGDDW GLFESITGYGLGGSSMALF
Sbjct: 181 KAFITAFRSGAVMGFLLSSSGLVVLYITINVFKMYYGDDWEGLFESITGYGLGGSSMALF 240

Query: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYA 302
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYA
Sbjct: 241 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYA 300

Query: 303 ESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPA 362
           ESSCAALVVASISSFG NH+ T M YPL+VSS+GI+VCL+TTLFATDFFEIKA  EIEPA
Sbjct: 301 ESSCAALVVASISSFGINHDFTAMCYPLLVSSVGIIVCLLTTLFATDFFEIKAANEIEPA 360

Query: 363 LKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGF 422
           LKKQLIIST LMT G+A+++WL++P+ FTIFNFG QK V+NW LF CVAVGLWAGLIIGF
Sbjct: 361 LKKQLIISTALMTVGVAVISWLALPAKFTIFNFGAQKEVSNWGLFFCVAVGLWAGLIIGF 420

Query: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 482
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSI+VSF+ AAMY
Sbjct: 421 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIYVSFSIAAMY 480

Query: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542
           GIA+AALGMLST+ATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 481 GIAMAALGMLSTMATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 540

Query: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602
           KGFAIGSAALVSLALFGAFVSRAGV  VDVL+PKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 541 KGFAIGSAALVSLALFGAFVSRAGVKVVDVLSPKVFIGLIVGAMLPYWFSAMTMKSVGSA 600

Query: 603 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG LF
Sbjct: 601 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGTLF 660

Query: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 722
           GVETLSGVLAG+LVSGVQIAISASNTGGAWDNAKKYIEAG SEHAR+LGPKGSD HKAAV
Sbjct: 661 GVETLSGVLAGALVSGVQIAISASNTGGAWDNAKKYIEAGNSEHARSLGPKGSDCHKAAV 720

Query: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFK 767
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA++GGLLFK
Sbjct: 721 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATYGGLLFK 760

BLAST of Carg27195-RA vs. Swiss-Prot
Match: sp|Q72Q29|HPPA_LEPIC (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) OX=267671 GN=hppA PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.7e-185
Identity = 386/697 (55.38%), Postives = 489/697 (70.16%), Query Frame = 0

Query: 75  KCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQPCTYDKTRTC 134
           K  EI SAISEGA +FL  EYK + +F+   AVLI + L +  S                
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGS---------------- 101

Query: 135 KPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 194
                  I++ ++F+ GA+ S +SGF+GMKIAT  N RT   A+  + KAF  AF SGAV
Sbjct: 102 -EGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 195 MGF---LLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYT 254
           MGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 MGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 255 KAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYAESSCAALVV 314
           KAADVGADLVGKVE+ IPEDDPRNPA IA   XXXXXXXX    DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIAXXXXXXXXXXXXXXXDLFGSCAEATCAALVI 281

Query: 315 ASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKKQLIIST 374
            + +S   +  +  +LYPL++S+ GI   ++T+  A     +K    +E ALK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 375 VLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 434
           +L+   +  VT   +  SF I      K +T W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 435 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGM 494
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 495 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 554
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 555 LVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 614
           L SLALF AF++R   T+++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 615 RQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 674
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GV 
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVX 641

Query: 675 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 734
                     AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 XXXXXXXXXXAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 701

Query: 735 KDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           KDTSGPS+NILIKLMA+ SLVFA FF   GGL+FKIF
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKIF 703

BLAST of Carg27195-RA vs. Swiss-Prot
Match: sp|Q8F641|HPPA_LEPIN (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=hppA PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.7e-185
Identity = 386/697 (55.38%), Postives = 489/697 (70.16%), Query Frame = 0

Query: 75  KCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTKPQPCTYDKTRTC 134
           K  EI SAISEGA +FL  EYK + +F+   AVLI + L +  S                
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGS---------------- 101

Query: 135 KPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 194
                  I++ ++F+ GA+ S +SGF+GMKIAT  N RT   A+  + KAF  AF SGAV
Sbjct: 102 -EGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 195 MGF---LLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYT 254
           MGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 MGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 255 KAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGSYAESSCAALVV 314
           KAADVGADLVGKVE+ IPEDDPRNPA IA   XXXXXXXX    DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIAXXXXXXXXXXXXXXXDLFGSCAEATCAALVI 281

Query: 315 ASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPALKKQLIIST 374
            + +S   +  +  +LYPL++S+ GI   ++T+  A     +K    +E ALK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 375 VLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 434
           +L+   +  VT   +  SF I      K +T W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 435 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGM 494
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 495 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 554
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 555 LVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 614
           L SLALF AF++R   T+++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 615 RQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 674
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GV 
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVX 641

Query: 675 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 734
                     AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 XXXXXXXXXXAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 701

Query: 735 KDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           KDTSGPS+NILIKLMA+ SLVFA FF   GGL+FKIF
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKIF 703

BLAST of Carg27195-RA vs. TrEMBL
Match: tr|O82680|O82680_CUCMO (Proton-translocating inorganic pyrophosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1)

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 765/768 (99.61%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           MSVTILPDLGTEIFIPVCAV+GIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASIS FGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISPFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGAS+HARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASKHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 768

BLAST of Carg27195-RA vs. TrEMBL
Match: tr|K7NFF3|K7NFF3_LAGSI (PHP1 OS=Lagenaria siceraria OX=3668 PE=2 SV=1)

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 740/768 (96.35%), Postives = 757/768 (98.57%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           MSVTILPDLG EIFIPVCA+VGI+FSLVQWYYVSQVKLSPGRD+A+NNSA +KNGYSDYL
Sbjct: 1   MSVTILPDLGAEIFIPVCAIVGILFSLVQWYYVSQVKLSPGRDSANNNSAAAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFM+LFAVLIFVFLGSVE FS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAVLIFVFLGSVEGFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQ C YDKT+TCKPALATA FST+SF+LGAVTSVVSGFLGMKIAT ANARTTLEARKG
Sbjct: 121 TKPQACLYDKTKTCKPALATAAFSTISFVLGAVTSVVSGFLGMKIATCANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHE TPMLYPLI+SSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEYTPMLYPLIISSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALKKQLIISTV+MTFGIA+VTWL+VPS+FTIFNFGTQKVV NW+LFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVIMTFGIALVTWLAVPSTFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGV  VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVHVVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 768

BLAST of Carg27195-RA vs. TrEMBL
Match: tr|A0A0A0LTJ8|A0A0A0LTJ8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G212840 PE=3 SV=1)

HSP 1 Score: 1367.8 bits (3539), Expect = 0.0e+00
Identity = 729/768 (94.92%), Postives = 750/768 (97.66%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           M  TILPDLG +IFIP+CA+VGI+FSLVQWYYVSQVKLS  RD+A+NNS+ +KNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFM+LFA LIFVFLGSVE FS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPC+YDKT+TCKPALATA FST+SFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYG+DWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHE T MLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALK QLIISTV+MTFGIAIVTW+SVP+ FTIFNFGTQKVV NW+LFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGV  VD+LTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGG+LFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of Carg27195-RA vs. TrEMBL
Match: tr|A0A1S3B8Q5|A0A1S3B8Q5_CUCME (pyrophosphate-energized vacuolar membrane proton pump OS=Cucumis melo OX=3656 GN=LOC103487401 PE=3 SV=1)

HSP 1 Score: 1366.3 bits (3535), Expect = 0.0e+00
Identity = 726/768 (94.53%), Postives = 750/768 (97.66%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           M  TILPDLG +IFIP+CA++GI+FSLVQWYYVSQVKLS  RD+A+NNS+ +KNGYSDYL
Sbjct: 1   MGATILPDLGAQIFIPLCAIIGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFM+LFA LIFVFLGSVE FS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPC+YDKT+TCKPALATA FST+SFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINIFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHE T MLYPLIVSSMGILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLVTTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALK QLIISTV+MTFGIAIVTW+ VP+ FTIFNFGTQKVV NW+LFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVGVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGV  VD+LTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVVDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGG+LFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

BLAST of Carg27195-RA vs. TrEMBL
Match: tr|K9JA38|K9JA38_CUCSA (Pyrophosphatase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 726/768 (94.53%), Postives = 748/768 (97.40%), Query Frame = 0

Query: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60
           M   ILPDLG +IFIP+CA+VGI+FSLVQWYYVSQVKLS  RD+A+NNS+ +KNGYSDYL
Sbjct: 1   MGAAILPDLGAQIFIPLCAIVGILFSLVQWYYVSQVKLSSARDSANNNSSSAKNGYSDYL 60

Query: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120
           IEEEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFM+LFA LIFVFLGSVE FS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMILFAALIFVFLGSVEGFS 120

Query: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPC+YDKT+TCKPALATA FST+SFLLGAVTSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCSYDKTKTCKPALATATFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYG+DWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGEDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVXXXXXXXXGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHE T MLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHEFTAMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420
           PALK QLIISTV+MTFGIAIVTW+SVP+ FTIFNFGTQKVV NW+LFLCVAVGLWAGLII
Sbjct: 361 PALKNQLIISTVIMTFGIAIVTWVSVPAKFTIFNFGTQKVVQNWELFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA SIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAASIFVSFSFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGV  +D+LTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVVVLDLLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHARTLGPKGSDPHK 
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGTSEHARTLGPKGSDPHKT 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKIF 769
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGG+LFKIF
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGILFKIF 768

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022929683.10.0e+00100.00pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata][more]
XP_023002108.10.0e+0099.74pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita maxima][more]
XP_023529940.10.0e+0099.74pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita pepo subsp... [more]
BAA33149.10.0e+0099.61proton-translocating inorganic pyrophosphatase [Cucurbita moschata][more]
XP_023515840.10.0e+0096.99pyrophosphate-energized vacuolar membrane proton pump [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
AT1G15690.10.0e+0087.97Inorganic H pyrophosphatase family protein[more]
AT1G16780.13.1e-11338.98Inorganic H pyrophosphatase family protein[more]
AT1G78920.16.8e-11339.09vacuolar H+-pyrophosphatase 2[more]
Match NameE-valueIdentityDescription
sp|P21616|AVP_VIGRR0.0e+0091.80Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radi... [more]
sp|P31414|AVP1_ARATH0.0e+0087.97Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana ... [more]
sp|Q06572|AVP_HORVU0.0e+0087.57Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513... [more]
sp|Q72Q29|HPPA_LEPIC1.7e-18555.38Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
sp|Q8F641|HPPA_LEPIN1.7e-18555.38Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Match NameE-valueIdentityDescription
tr|O82680|O82680_CUCMO0.0e+0099.61Proton-translocating inorganic pyrophosphatase OS=Cucurbita moschata OX=3662 GN=... [more]
tr|K7NFF3|K7NFF3_LAGSI0.0e+0096.35PHP1 OS=Lagenaria siceraria OX=3668 PE=2 SV=1[more]
tr|A0A0A0LTJ8|A0A0A0LTJ8_CUCSA0.0e+0094.92Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G212840 PE=3 SV=1[more]
tr|A0A1S3B8Q5|A0A1S3B8Q5_CUCME0.0e+0094.53pyrophosphate-energized vacuolar membrane proton pump OS=Cucumis melo OX=3656 GN... [more]
tr|K9JA38|K9JA38_CUCSA0.0e+0094.53Pyrophosphatase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
The following terms have been associated with this mRNA:
Vocabulary: Molecular Function
TermDefinition
GO:0004427inorganic diphosphatase activity
GO:0009678hydrogen-translocating pyrophosphatase activity
Vocabulary: Biological Process
TermDefinition
GO:1902600hydrogen ion transmembrane transport
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR004131PPase-energised_H-pump
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 hydrogen ion transmembrane transport
biological_process GO:0006119 oxidative phosphorylation
cellular_component GO:0016020 membrane
molecular_function GO:0009678 hydrogen-translocating pyrophosphatase activity
molecular_function GO:0004427 inorganic diphosphatase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg27195Carg27195gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27195-RA.three_prime_UTR.1Carg27195-RA.three_prime_UTR.1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27195-RA.CDS.8Carg27195-RA.CDS.8CDS
Carg27195-RA.CDS.7Carg27195-RA.CDS.7CDS
Carg27195-RA.CDS.6Carg27195-RA.CDS.6CDS
Carg27195-RA.CDS.5Carg27195-RA.CDS.5CDS
Carg27195-RA.CDS.4Carg27195-RA.CDS.4CDS
Carg27195-RA.CDS.3Carg27195-RA.CDS.3CDS
Carg27195-RA.CDS.2Carg27195-RA.CDS.2CDS
Carg27195-RA.CDS.1Carg27195-RA.CDS.1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27195-RA.five_prime_UTR.1Carg27195-RA.five_prime_UTR.1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg27195-RA.exon.8Carg27195-RA.exon.8exon
Carg27195-RA.exon.7Carg27195-RA.exon.7exon
Carg27195-RA.exon.6Carg27195-RA.exon.6exon
Carg27195-RA.exon.5Carg27195-RA.exon.5exon
Carg27195-RA.exon.4Carg27195-RA.exon.4exon
Carg27195-RA.exon.3Carg27195-RA.exon.3exon
Carg27195-RA.exon.2Carg27195-RA.exon.2exon
Carg27195-RA.exon.1Carg27195-RA.exon.1exon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg27195-RACarg27195-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004131Pyrophosphate-energised proton pumpPIRSFPIRSF001265H+-PPtasecoord: 11..766
e-value: 7.8E-283
score: 938.3
IPR004131Pyrophosphate-energised proton pumpTIGRFAMTIGR01104TIGR01104coord: 11..763
e-value: 0.0
score: 1229.5
IPR004131Pyrophosphate-energised proton pumpPFAMPF03030H_PPasecoord: 22..753
e-value: 6.8E-259
score: 860.8
IPR004131Pyrophosphate-energised proton pumpPANTHERPTHR31998FAMILY NOT NAMEDcoord: 3..767
IPR004131Pyrophosphate-energised proton pumpHAMAPMF_01129PPase_energized_pumpcoord: 9..758
score: 23.103
NoneNo IPR availablePANTHERPTHR31998:SF10SUBFAMILY NOT NAMEDcoord: 3..767