Carg27339 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg27339
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	IST1-like protein
Location	Cucurbita_argyrosperma_scaffold_326 : 92174 .. 92810 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAAAACTGAGAAGAGTCTTTAAACCTCTCCTGGGAAGAAGCAGGTCCTCTAGTTTCAGACGTTTAGTTCAAAACGCTTTGGAGCACCTCGAAACCCTAAAGAAGCTTCGCCATGGCCGGTACGTCGAAGATCGAAAGGACGCCGCTCGTCTCCTTCATGAAGGCCTTCCCAAACTAGCCCTCTCTCGCGTACGTCTCTCATCCCCTTTTCGATTAACTTTCAGATCATTCGACATAAATTCTCGATGATGTGCGCAGTGCGAGCAAATGTTTCGACATCAAAATCTAATGGATGCTTATGGCATGATGGAAGGGTATCTAAATCTGTTGAGGGAAAGATTGTACCTCCTTGCACCTGGAAGGTTTGCTACTTTACAATGAATTTTCATAATTAAATTCCTATTTTTTCAAAAGGAAAATTTGAAAATAGTATTGATGTTTACCTAGAGAGTGTCCTAAAGAATTGGAGGAGGCAGTGTCGAGTGTGGTATTTGCAGCTTCAAGATGTGAGGACTTTCCAGAGCTTGAAGAGATTAAATCAGTATTGAGTTCTAGGTTTGGCACAGAGTTTGCTGCTCGTGCTGTTGAATTACGCAACAATAATACTGTAAATCACTCGGTATGTCTCACTTAA

mRNA sequence

ATGAGAAAACTGAGAAGAGTCTTTAAACCTCTCCTGGGAAGAAGCAGGTCCTCTAGTTTCAGACGTTTAGTTCAAAACGCTTTGGAGCACCTCGAAACCCTAAAGAAGCTTCGCCATGGCCGGTACGTCGAAGATCGAAAGGACGCCGCTCGTCTCCTTCATGAAGGCCTTCCCAAACTAGCCCTCTCTCGCTGCGAGCAAATGTTTCGACATCAAAATCTAATGGATGCTTATGGCATGATGGAAGGGTATCTAAATCTGTTGAGGGAAAGATTGTACCTCCTTGCACCTGGAAGAGAGTGTCCTAAAGAATTGGAGGAGGCAGTGTCGAGTGTGGTATTTGCAGCTTCAAGATGTGAGGACTTTCCAGAGCTTGAAGAGATTAAATCAGTATTGAGTTCTAGGTTTGGCACAGAGTTTGCTGCTCGTGCTGTTGAATTACGCAACAATAATACTGTAAATCACTCGGTATGTCTCACTTAA

Coding sequence (CDS)

ATGAGAAAACTGAGAAGAGTCTTTAAACCTCTCCTGGGAAGAAGCAGGTCCTCTAGTTTCAGACGTTTAGTTCAAAACGCTTTGGAGCACCTCGAAACCCTAAAGAAGCTTCGCCATGGCCGGTACGTCGAAGATCGAAAGGACGCCGCTCGTCTCCTTCATGAAGGCCTTCCCAAACTAGCCCTCTCTCGCTGCGAGCAAATGTTTCGACATCAAAATCTAATGGATGCTTATGGCATGATGGAAGGGTATCTAAATCTGTTGAGGGAAAGATTGTACCTCCTTGCACCTGGAAGAGAGTGTCCTAAAGAATTGGAGGAGGCAGTGTCGAGTGTGGTATTTGCAGCTTCAAGATGTGAGGACTTTCCAGAGCTTGAAGAGATTAAATCAGTATTGAGTTCTAGGTTTGGCACAGAGTTTGCTGCTCGTGCTGTTGAATTACGCAACAATAATACTGTAAATCACTCGGTATGTCTCACTTAA

Protein sequence

MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCLT

Homology

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BLAST of Carg27339 vs. NCBI nr
Match: XP_022963468.1 (uncharacterized protein LOC111463790 [Cucurbita moschata])

HSP 1 Score: 306.6 bits (784), Expect = 4.9e-80
Identity = 157/159 (98.74%), Postives = 158/159 (99.37%), Query Frame = 0


Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120
           ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE
Sbjct: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120

Query: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHS+ L
Sbjct: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSIML 159

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BLAST of Carg27339 vs. NCBI nr
Match: XP_023538969.1 (uncharacterized protein LOC111799739 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.5 bits (763), Expect = 1.3e-77
Identity = 154/159 (96.86%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120
           ALSRCEQM RHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE
Sbjct: 61  ALSRCEQMIRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120

Query: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           DFPELEEIKSVLSSRFG EFAARAVELR NNTVNHS+ L
Sbjct: 121 DFPELEEIKSVLSSRFGREFAARAVELRINNTVNHSIML 159

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BLAST of Carg27339 vs. NCBI nr
Match: XP_023522757.1 (uncharacterized protein LOC111786761, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 296.2 bits (757), Expect = 6.7e-77
Identity = 153/156 (98.08%), Postives = 153/156 (98.08%), Query Frame = 0


Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120
           ALSRCEQM RHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE
Sbjct: 61  ALSRCEQMIRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE 120

Query: 121 DFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHS 157
           DFPELEEIKSVLSSRFG EFAARAVELR NNTVNHS
Sbjct: 121 DFPELEEIKSVLSSRFGREFAARAVELRINNTVNHS 156

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BLAST of Carg27339 vs. NCBI nr
Match: XP_022971780.1 (uncharacterized protein LOC111470458 [Cucurbita maxima])

HSP 1 Score: 292.4 bits (747), Expect = 9.6e-76
Identity = 151/160 (94.38%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKL 60
           MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKL HGRYVEDRKD ARLLHEGLPKL
Sbjct: 1   MRKLRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLHHGRYVEDRKDTARLLHEGLPKL 60

Query: 61  ALSRCEQMFRHQNLMDAYGMMEGYLNLLRERLY-LLAPGRECPKELEEAVSSVVFAASRC 120
           ALSRCEQM RHQNLMDAYGMMEGYLNLLRER++ LLAPGRECPKELEEAVSSVVFAASRC
Sbjct: 61  ALSRCEQMIRHQNLMDAYGMMEGYLNLLRERMHLLLAPGRECPKELEEAVSSVVFAASRC 120

Query: 121 EDFPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           EDFPEL+EIKSVLSSRFGTEFAARAVELRNNNTVNHS+ L
Sbjct: 121 EDFPELQEIKSVLSSRFGTEFAARAVELRNNNTVNHSIML 160

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BLAST of Carg27339 vs. NCBI nr
Match: XP_022139637.1 (uncharacterized protein LOC111010490 [Momordica charantia])

HSP 1 Score: 190.7 bits (483), Expect = 4.0e-45
Identity = 99/153 (64.71%), Postives = 121/153 (79.08%), Query Frame = 0


Query: 4   LRRVFKPLLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLL-HEGLPKLA 63
           +RR+  PLLGR+ +SS+FRRL+ +A   L++L   R  RY +DR D  RLL  +GL  LA
Sbjct: 1   MRRILNPLLGRTFKSSNFRRLIHSAFTRLQSLNTHRRRRYSQDRDDVVRLLQQDGLHNLA 60

Query: 64  LSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCED 123
           LSRCEQ+ +HQNL+DAYGM+EGYLNLL +R+YLL   RECP EL+EA S VV+AASRC D
Sbjct: 61  LSRCEQVIKHQNLLDAYGMIEGYLNLLMDRIYLLVQQRECPDELKEAASGVVYAASRCRD 120

Query: 124 FPELEEIKSVLSSRFGTEFAARAVELRNNNTVN 155
           FPELEEIKSVL+SRFG EF+ RAVELRNN+ VN
Sbjct: 121 FPELEEIKSVLTSRFGREFSGRAVELRNNSGVN 153

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BLAST of Carg27339 vs. TAIR10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 110.9 bits (276), Expect = 7.2e-25
Identity = 63/148 (42.57%), Postives = 92/148 (62.16%), Query Frame = 0


Query: 11  LLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMF 70
           LLGRS +++ F+ L+  AL  L  LK  R  R  +   D   LL  G  + A  R +Q+ 
Sbjct: 8   LLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAYHRVDQVV 67

Query: 71  RHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIK 130
           + QN +D    + GY  L  +R++L    R+CP+EL EAVS ++FAASR  +FPEL+EI+
Sbjct: 68  KDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEFPELQEIR 127

Query: 131 SVLSSRFGTEFAARAVELRNNNTVNHSV 158
           +VL SRFG + AAR++ELR+N  V+  +
Sbjct: 128 NVLISRFGKDLAARSIELRSNCGVDPKI 155

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BLAST of Carg27339 vs. TAIR10
Match: AT1G34220.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 86.7 bits (213), Expect = 1.5e-17
Identity = 45/139 (32.37%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 16  RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLM 75
           +++  + L++  +  ++ ++  R  +  + R++ A+LL  G    A  R E + R + +M
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 76  DAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSR 135
            A  ++E +  L+  RL ++   RECP +L+EA+SSV FAA RC D  EL++++ +  S+
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 136 FGTEFAARAVELRNNNTVN 155
           +G EF A A EL+ ++ VN
Sbjct: 133 YGKEFVAAASELKPDSGVN 151

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BLAST of Carg27339 vs. TAIR10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 86.7 bits (213), Expect = 1.5e-17
Identity = 44/131 (33.59%), Postives = 77/131 (58.78%), Query Frame = 0


Query: 27  ALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMMEGYLN 86
           A+  ++ L+  R  +    +K+ A  L  G   +A  R E + R  NL  AY ++E +  
Sbjct: 24  AIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIREMNLWAAYEILELFCE 83

Query: 87  LLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSRFGTEFAARAVE 146
            +  R+ +L   +ECP+EL EA++S++FAA RC + P+L +IK++  +++G EF   A E
Sbjct: 84  FILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLFGTKYGKEFIMVASE 143

Query: 147 LRNNNTVNHSV 158
           LR ++ VN ++
Sbjct: 144 LRPDSGVNRTI 154

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BLAST of Carg27339 vs. TAIR10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 78.6 bits (192), Expect = 4.0e-15
Identity = 51/151 (33.77%), Postives = 84/151 (55.63%), Query Frame = 0


Query: 4   LRRVFKPLLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALS 63
           L+R FKP       +  +  +Q A   L+ LK  +  +  + R++ A+LL  G    A  
Sbjct: 5   LQRGFKP-------AKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARI 64

Query: 64  RCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFP 123
           R E + R +  + AY ++  Y  LL  RL ++   + CP +L+EAV+SV+FA+ R  D P
Sbjct: 65  RVEHVVREEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVP 124

Query: 124 ELEEIKSVLSSRFGTEFAARAVELRNNNTVN 155
           EL EI    ++++G +F+  AVELR ++ V+
Sbjct: 125 ELSEIFKQFTTKYGKDFSTSAVELRPDSGVS 148

[Close]

BLAST of Carg27339 vs. TAIR10
Match: AT2G14830.1 (Regulator of Vps4 activity in the MVB pathway protein)

HSP 1 Score: 78.2 bits (191), Expect = 5.2e-15
Identity = 48/147 (32.65%), Postives = 76/147 (51.70%), Query Frame = 0


Query: 11  LLGRSRSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFR 70
           L G  R+S  + +V+     L  LK  ++      R D A+LL  G    AL R +Q+F 
Sbjct: 75  LFGWRRTSKCKSVVKQLQCRLNLLKNKKYAISSHLRNDIAQLLRIGERDRALHRAQQLFL 134

Query: 71  HQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKS 130
            +NLM  Y ++  + +++   L  +   R+ P  + EAVS++VFA++RC D PEL  ++ 
Sbjct: 135 DENLMSLYHLLLHFSDIILLNLSYIRRRRDLPDGINEAVSTLVFASARCGDLPELRALRV 194

Query: 131 VLSSRFGTEFAARAVELRNNNTVNHSV 158
           +   R+G  F   A+ L   N VN  V
Sbjct: 195 LFGKRYGNHFVDTALNLLPGNCVNPQV 221

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BLAST of Carg27339 vs. Swiss-Prot
Match: sp|Q54I39|IST1L_DICDI (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.4e-06
Identity = 30/111 (27.03%), Postives = 65/111 (58.56%), Query Frame = 0


Query: 27  ALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMMEGYLN 86
           A+  ++ LK  +     +++++ A LL +   + A  R E + R + L++ + ++E    
Sbjct: 20  AVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECFQIIEVLCE 79

Query: 87  LLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIKSVLSSRFG 138
           LL  R+ L+    E P E++E++ ++V+++ R +  PELE+IK+ L +++G
Sbjct: 80  LLHARINLINATTEMPLEMKESIFTLVYSSQRIQ-IPELEQIKNQLKAKYG 129

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BLAST of Carg27339 vs. Swiss-Prot
Match: sp|Q3ZBV1|IST1_BOVIN (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.8e-04
Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 22  RLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMM 81
           RLV N L+ LE   K +     + RK+ A  L  G  + A  R E + R   L++A  ++
Sbjct: 16  RLVINRLKLLE---KKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEIL 75

Query: 82  EGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE-DFPELEEIKSVLSSRFGTEF 141
           E Y +LL  R  L+   +E    L E+VS++++AA R + +  EL+ +   L +++  E+
Sbjct: 76  ELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEY 132

[Close]

BLAST of Carg27339 vs. Swiss-Prot
Match: sp|P53990|IST1_HUMAN (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.8e-04
Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 22  RLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMM 81
           RLV N L+ LE   K +     + RK+ A  L  G  + A  R E + R   L++A  ++
Sbjct: 16  RLVINRLKLLE---KKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEIL 75

Query: 82  EGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE-DFPELEEIKSVLSSRFGTEF 141
           E Y +LL  R  L+   +E    L E+VS++++AA R + +  EL+ +   L +++  E+
Sbjct: 76  ELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEY 132

[Close]

BLAST of Carg27339 vs. Swiss-Prot
Match: sp|Q9CX00|IST1_MOUSE (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.8e-04
Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 22  RLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMM 81
           RLV N L+ LE   K +     + RK+ A  L  G  + A  R E + R   L++A  ++
Sbjct: 16  RLVINRLKLLE---KKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEIL 75

Query: 82  EGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE-DFPELEEIKSVLSSRFGTEF 141
           E Y +LL  R  L+   +E    L E+VS++++AA R + +  EL+ +   L +++  E+
Sbjct: 76  ELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEY 132

[Close]

BLAST of Carg27339 vs. Swiss-Prot
Match: sp|Q568Z6|IST1_RAT (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.8e-04
Identity = 37/120 (30.83%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 22  RLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMFRHQNLMDAYGMM 81
           RLV N L+ LE   K +     + RK+ A  L  G  + A  R E + R   L++A  ++
Sbjct: 16  RLVINRLKLLE---KKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEIL 75

Query: 82  EGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCE-DFPELEEIKSVLSSRFGTEF 141
           E Y +LL  R  L+   +E    L E+VS++++AA R + +  EL+ +   L +++  E+
Sbjct: 76  ELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEY 132

[Close]

BLAST of Carg27339 vs. TrEMBL
Match: tr|A0A1S3BD77|A0A1S3BD77_CUCME (uncharacterized protein LOC103488605 OS=Cucumis melo OX=3656 GN=LOC103488605 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.6e-42
Identity = 95/155 (61.29%), Postives = 117/155 (75.48%), Query Frame = 0


Query: 4   LRRVFKPLLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLAL 63
           +RR+   ++GRS RSS F+RLVQ++   L++L   R  RY ED  D   LL +GL KLAL
Sbjct: 1   MRRILNRVVGRSFRSSGFKRLVQSSFTRLQSLTDHRRRRYSEDLNDVLSLLQDGLHKLAL 60

Query: 64  SRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDF 123
           SRCEQM +HQNL+DAYG++EGYLNLL ER+ LL   RECP EL+EA SSVVFAASR +DF
Sbjct: 61  SRCEQMIKHQNLLDAYGLIEGYLNLLMERIQLLGRARECPDELKEAASSVVFAASRWKDF 120

Query: 124 PELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSV 158
            EL +IKS+ +S+FG EF A AVELRNNN VN S+
Sbjct: 121 TELGDIKSIFTSQFGKEFTAPAVELRNNNRVNQSI 155

[Close]

BLAST of Carg27339 vs. TrEMBL
Match: tr|A0A0A0K949|A0A0A0K949_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G031970 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.5e-40
Identity = 92/155 (59.35%), Postives = 119/155 (76.77%), Query Frame = 0


Query: 4   LRRVFKPLLGRSRSSS-FRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLAL 63
           +R++ K +LGRS SSS F+R VQ++   L++L   R  R+ ED  +   LL E L +LAL
Sbjct: 1   MRKIMKRVLGRSFSSSCFKRSVQSSFTRLKSLTDHRRRRFSEDLNEVVSLLQEDLQELAL 60

Query: 64  SRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDF 123
           SRCEQM +HQNL+DAYG++EGYLNLL ER++LL   RECP EL+EAVSSV+FAASR +DF
Sbjct: 61  SRCEQMIKHQNLVDAYGLIEGYLNLLIERIHLLGRERECPDELKEAVSSVIFAASRWKDF 120

Query: 124 PELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSV 158
            EL ++KS+ +S+FG EF ARAVELRNNN VN S+
Sbjct: 121 TELGDVKSIFTSQFGKEFTARAVELRNNNRVNQSI 155

[Close]

BLAST of Carg27339 vs. TrEMBL
Match: tr|A0A2P5F9H5|A0A2P5F9H5_9ROSA (Vacuolar protein sorting-associated protein OS=Trema orientalis OX=63057 GN=TorRG33x02_096920 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.8e-31
Identity = 78/158 (49.37%), Postives = 105/158 (66.46%), Query Frame = 0


Query: 4   LRRVFKPLLGRS--RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLA 63
           +R     LLGRS  ++S F+ LV  A+  L  LK  R  R  + R D  +LL  G    A
Sbjct: 1   MRNKINSLLGRSLIKTSKFKPLVSLAISRLAVLKNQRQVRSSQARSDVVQLLQLGHHDRA 60

Query: 64  LSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCED 123
           L R E + + QN++D Y M+EGY NLL ER++L+   R CP EL+EA+S +++AASRC D
Sbjct: 61  LLRVEHLIKEQNMLDVYDMIEGYCNLLIERIHLIEHERVCPDELKEAISGLLYAASRCGD 120

Query: 124 FPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           FPEL EI++VL+S FG EFAARA+ELRNN  VNH++ +
Sbjct: 121 FPELHEIRAVLTSHFGKEFAARAIELRNNCGVNHTIII 158

[Close]

BLAST of Carg27339 vs. TrEMBL
Match: tr|A0A1Q3AUG0|A0A1Q3AUG0_CEPFO (Ist1 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_02724 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-30
Identity = 74/146 (50.68%), Postives = 104/146 (71.23%), Query Frame = 0


Query: 11  LLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLALSRCEQMF 70
           LLGR+ +++ F+ L   A+  L  LK+ RH R  + R D  +LL +G  + AL R EQ+ 
Sbjct: 8   LLGRTFKTNKFKPLANLAISRLAVLKQQRHVRCSQARSDVLQLLEQGHHERALLRVEQVI 67

Query: 71  RHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDFPELEEIK 130
           + QN++D + M E Y NL+ ER++L+   RECP EL+EA+SS++FAASRC DFPEL+EI+
Sbjct: 68  KEQNMLDVFVMTESYCNLVIERVHLIEQERECPDELKEAISSLLFAASRCGDFPELQEIR 127

Query: 131 SVLSSRFGTEFAARAVELRNNNTVNH 156
            + +SR+G EFAARA+ELRNN  VNH
Sbjct: 128 VLFTSRYGKEFAARAIELRNNCGVNH 153

[Close]

BLAST of Carg27339 vs. TrEMBL
Match: tr|A0A2P5CER8|A0A2P5CER8_PARAD (Vacuolar protein sorting-associated protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_159000 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.1e-30
Identity = 76/158 (48.10%), Postives = 104/158 (65.82%), Query Frame = 0


Query: 4   LRRVFKPLLGRS--RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLA 63
           +R     LLGRS  ++S F+ LV  A+  L  LK  R  R  + R D  +LL  G    A
Sbjct: 1   MRNKINSLLGRSLIKTSKFKPLVSLAISRLAVLKNQRQVRSSQARSDVVQLLQLGHHDRA 60

Query: 64  LSRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCED 123
           L R E + + QN++D Y M+EGY NLL ER++L+   R CP EL+EA+S +++A SRC D
Sbjct: 61  LLRVEHVIKEQNMLDVYDMIEGYCNLLIERIHLIEHERVCPDELKEAISGLLYADSRCGD 120

Query: 124 FPELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSVCL 160
           FPEL EI++VL+S FG E AARA+ELRNN+ VNH++ +
Sbjct: 121 FPELHEIRAVLTSHFGKEIAARAIELRNNSGVNHTIII 158

The following BLAST results are available for this feature:

BLAST of Carg27339 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022963468.1	4.9e-80	98.74	uncharacterized protein LOC111463790 [Cucurbita moschata]	[more]
XP_023538969.1	1.3e-77	96.86	uncharacterized protein LOC111799739 [Cucurbita pepo subsp. pepo]	[more]
XP_023522757.1	6.7e-77	98.08	uncharacterized protein LOC111786761, partial [Cucurbita pepo subsp. pepo]	[more]
XP_022971780.1	9.6e-76	94.38	uncharacterized protein LOC111470458 [Cucurbita maxima]	[more]
XP_022139637.1	4.0e-45	64.71	uncharacterized protein LOC111010490 [Momordica charantia]	[more]

BLAST of Carg27339 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G13340.1	7.2e-25	42.57	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.1	1.5e-17	32.37	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G25420.1	1.5e-17	33.59	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT2G19710.1	4.0e-15	33.77	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT2G14830.1	5.2e-15	32.65	Regulator of Vps4 activity in the MVB pathway protein	[more]

BLAST of Carg27339 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q54I39\|IST1L_DICDI	1.4e-06	27.03	IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1	[more]
sp\|Q3ZBV1\|IST1_BOVIN	8.8e-04	30.83	IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1	[more]
sp\|P53990\|IST1_HUMAN	8.8e-04	30.83	IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1	[more]
sp\|Q9CX00\|IST1_MOUSE	8.8e-04	30.83	IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1	[more]
sp\|Q568Z6\|IST1_RAT	8.8e-04	30.83	IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1	[more]

BLAST of Carg27339 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BD77\|A0A1S3BD77_CUCME	4.6e-42	61.29	uncharacterized protein LOC103488605 OS=Cucumis melo OX=3656 GN=LOC103488605 PE=...	[more]
tr\|A0A0A0K949\|A0A0A0K949_CUCSA	1.5e-40	59.35	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G031970 PE=4 SV=1	[more]
tr\|A0A2P5F9H5\|A0A2P5F9H5_9ROSA	2.8e-31	49.37	Vacuolar protein sorting-associated protein OS=Trema orientalis OX=63057 GN=TorR...	[more]
tr\|A0A1Q3AUG0\|A0A1Q3AUG0_CEPFO	1.8e-30	50.68	Ist1 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_027...	[more]
tr\|A0A2P5CER8\|A0A2P5CER8_PARAD	3.1e-30	48.10	Vacuolar protein sorting-associated protein OS=Parasponia andersonii OX=3476 GN=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0015031	protein transport

Vocabulary: **INTERPRO**
Term	Definition
IPR005061	Ist1

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27339-RA	Carg27339-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:1.20.1260.60		coord: 11..159 e-value: 8.0E-37 score: 128.5
None	No IPR available	PANTHER	PTHR12161:SF16	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 9..156
IPR005061	Vacuolar protein sorting-associated protein Ist1	PFAM	PF03398	Ist1	coord: 23..157 e-value: 1.2E-33 score: 116.2
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	UNCHARACTERIZED DUF292	coord: 9..156

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg27339	MELO3C011552	Melon (DHL92) v3.5.1	carmeB0491
Carg27339	Cucsa.365400	Cucumber (Gy14) v1	carcgyB0557
Carg27339	CmaCh04G020580	Cucurbita maxima (Rimu)	carcmaB0675
Carg27339	CmoCh04G021560	Cucurbita moschata (Rifu)	carcmoB0656
Carg27339	Cp4.1LG01g17960	Cucurbita pepo (Zucchini)	carcpeB0680
Carg27339	MELO3C029971.2	Melon (DHL92) v3.6.1	carmedB0497

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg27339	Cucumber (Gy14) v2	carcgybB0493
Carg27339	Wild cucumber (PI 183967)	carcpiB0523
Carg27339	Cucumber (Chinese Long) v2	carcuB0516
Carg27339	Cucumber (Chinese Long) v3	carcucB0522
Carg27339	Bottle gourd (USVL1VR-Ls)	carlsiB439
Carg27339	Watermelon (Charleston Gray)	carwcgB0469
Carg27339	Watermelon (97103) v1	carwmB0481
Carg27339	Watermelon (97103) v2	carwmbB0508