Carg25395-RA (mRNA) Silver-seed gourd

NameCarg25395-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionWRKY family transcription factor
LocationCucurbita_argyrosperma_scaffold_232 : 194118 .. 195920 (+)
Sequence length1170
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTGCAATTTGTTTGAGATGGACAATGATAACTATCAGCAAGCGGGGGATTTGACCGATGTAATTCGAGCTAGTTCCGCCGCCACCTCAGCTGCCCACTCATCGGAATTCTCTCTCGGCGACCGCCATCTCTTGCCTCACCTTCCCGCCGATGACTCTTCCATGAATTTTGGAGATCCATTGTTGCTTCCCACTTCTAGTACTCGAGATCCGTTTCTTCATCCCCATTTCTCTTCCTTCAATTTTGATTCTCACGGCGGCGGTTGGGGTTTCACGGCGGCGGAACGGCCTTCTACTGTATTTCCAAGAATGTTGCAGATCTCTCCCACCTCCAAAGTTGGTGACGGTGTAATTACTACTCCTCCATGCGACTCACCGGCCGTGGCCGTAACGGGGAATTCTCCGACCTCGAACCCGCTTTGCTTAAGGGATAATTCCGACCTCCAGATCTCTCCTCCAAGAAATCCCGCCCTTAAAAGAAGGTATAAGAACAACCATAGCGTTTATATCTAATCAATCATAGTTATTAAACAATAACGAATCTTCGATTATTAGTATTCTAAATTTCGTTTTAGTTCGATTTTTGCTCTAATTTCATGTGGGTTCAAAACGAATTCAAATTTTAGATTAAGTTCGACAATTATTGATTAATTAAAGAGTTTAAAGTTCGAAAAAACACTAAATCCTAATCAAACCATGACTAAAATTTCATTCATGGAATGTTCATAGGAAGAACCAAGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCAAACAGCCGATCAAGCAGCGGCGAAGTTGTCCCCTCCGATCTATGGGCATGGAGGAAGTACGGACAAAAACCCATCAAAGGATCTCCATATCCAAGGTCAATTATACTTCAATTATTTTCCTTAAATTAAAATAAATAAATATATATATACATATATCTATATATATATACATATATCTATATATATATATATATATATATATAATTTTGTTTTCCAGAAATCAATGCCAAATGCTGCAAGTTTTTGTACCTCTTAATTTTCATCTGACAAGAGATTGACTTCCAATAATTTTATTCATCATTTACTCATCAAATTCTCAAGTGATTTGTAAAAATTTAATAATTTATATAATTATTGTAAGAAAATTATTGTTTTGTTCTTTAAATTTTAGATCTTTTTTATGAGCTGATAATTAGTATTATCATTTGAATTAAGAGAATTTATTAGTGAAAATATTGCCCTTCCTTTCTCTTTTCTTTAGGGGATATTACAGGTGTAGCAGCTCCAAAGGCTGCTCGGCCCGAAAGCAAGTCGAGCGTAGCCGAACCGACCCCAACATGCTCGTGATCACCTACACTTCGGAGCATAACCACCCATGGCCTACTCAACGTAATGCACTAGCAGGCTCAACTCGCTCCCACCCTTCTCGAACCTCCACTTCTACCACACGTATAACAAGCCCAAAACATGAGCACAACAATGAAAACCTAGCAACTGTGACGATAAAGGAGGAGGACATGAATGAAGACCAAACGACGGAGAAAGCAACCACAACAATAACAACAAACCAACATCAGGTCGAGGGAGATCATCAAGAGTTCTTGTACGACTTGATTTTCGACGAATTTGCTGATCAAATGAACGAGCATGATCGTCATCATGCAACGGACACGATGAACGATAACCTTCTCCGGTTTAGTAATCATGGGTTTAGTAAAGGAAATGATGAAGAGCGCAGTAAAGATCCATTTTTGGAGCTCTATGATTGGACAGAGAAGTCAAATGGGAGCTTGTTCAAATAA

mRNA sequence

ATGTTCTGCAATTTGTTTGAGATGGACAATGATAACTATCAGCAAGCGGGGGATTTGACCGATGTAATTCGAGCTAGTTCCGCCGCCACCTCAGCTGCCCACTCATCGGAATTCTCTCTCGGCGACCGCCATCTCTTGCCTCACCTTCCCGCCGATGACTCTTCCATGAATTTTGGAGATCCATTGTTGCTTCCCACTTCTAGTACTCGAGATCCGTTTCTTCATCCCCATTTCTCTTCCTTCAATTTTGATTCTCACGGCGGCGGTTGGGGTTTCACGGCGGCGGAACGGCCTTCTACTGTATTTCCAAGAATGTTGCAGATCTCTCCCACCTCCAAAGTTGGTGACGGTGTAATTACTACTCCTCCATGCGACTCACCGGCCGTGGCCGTAACGGGGAATTCTCCGACCTCGAACCCGCTTTGCTTAAGGGATAATTCCGACCTCCAGATCTCTCCTCCAAGAAATCCCGCCCTTAAAAGAAGGAAGAACCAAGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCAAACAGCCGATCAAGCAGCGGCGAAGTTGTCCCCTCCGATCTATGGGCATGGAGGAAGTACGGACAAAAACCCATCAAAGGATCTCCATATCCAAGGGGATATTACAGGTGTAGCAGCTCCAAAGGCTGCTCGGCCCGAAAGCAAGTCGAGCGTAGCCGAACCGACCCCAACATGCTCGTGATCACCTACACTTCGGAGCATAACCACCCATGGCCTACTCAACGTAATGCACTAGCAGGCTCAACTCGCTCCCACCCTTCTCGAACCTCCACTTCTACCACACGTATAACAAGCCCAAAACATGAGCACAACAATGAAAACCTAGCAACTGTGACGATAAAGGAGGAGGACATGAATGAAGACCAAACGACGGAGAAAGCAACCACAACAATAACAACAAACCAACATCAGGTCGAGGGAGATCATCAAGAGTTCTTGTACGACTTGATTTTCGACGAATTTGCTGATCAAATGAACGAGCATGATCGTCATCATGCAACGGACACGATGAACGATAACCTTCTCCGGTTTAGTAATCATGGGTTTAGTAAAGGAAATGATGAAGAGCGCAGTAAAGATCCATTTTTGGAGCTCTATGATTGGACAGAGAAGTCAAATGGGAGCTTGTTCAAATAA

Coding sequence (CDS)

ATGTTCTGCAATTTGTTTGAGATGGACAATGATAACTATCAGCAAGCGGGGGATTTGACCGATGTAATTCGAGCTAGTTCCGCCGCCACCTCAGCTGCCCACTCATCGGAATTCTCTCTCGGCGACCGCCATCTCTTGCCTCACCTTCCCGCCGATGACTCTTCCATGAATTTTGGAGATCCATTGTTGCTTCCCACTTCTAGTACTCGAGATCCGTTTCTTCATCCCCATTTCTCTTCCTTCAATTTTGATTCTCACGGCGGCGGTTGGGGTTTCACGGCGGCGGAACGGCCTTCTACTGTATTTCCAAGAATGTTGCAGATCTCTCCCACCTCCAAAGTTGGTGACGGTGTAATTACTACTCCTCCATGCGACTCACCGGCCGTGGCCGTAACGGGGAATTCTCCGACCTCGAACCCGCTTTGCTTAAGGGATAATTCCGACCTCCAGATCTCTCCTCCAAGAAATCCCGCCCTTAAAAGAAGGAAGAACCAAGTGAAGAAGGTGGTGTGCATTCCGGCGCCGGCGCCGGCAAACAGCCGATCAAGCAGCGGCGAAGTTGTCCCCTCCGATCTATGGGCATGGAGGAAGTACGGACAAAAACCCATCAAAGGATCTCCATATCCAAGGGGATATTACAGGTGTAGCAGCTCCAAAGGCTGCTCGGCCCGAAAGCAAGTCGAGCGTAGCCGAACCGACCCCAACATGCTCGTGATCACCTACACTTCGGAGCATAACCACCCATGGCCTACTCAACGTAATGCACTAGCAGGCTCAACTCGCTCCCACCCTTCTCGAACCTCCACTTCTACCACACGTATAACAAGCCCAAAACATGAGCACAACAATGAAAACCTAGCAACTGTGACGATAAAGGAGGAGGACATGAATGAAGACCAAACGACGGAGAAAGCAACCACAACAATAACAACAAACCAACATCAGGTCGAGGGAGATCATCAAGAGTTCTTGTACGACTTGATTTTCGACGAATTTGCTGATCAAATGAACGAGCATGATCGTCATCATGCAACGGACACGATGAACGATAACCTTCTCCGGTTTAGTAATCATGGGTTTAGTAAAGGAAATGATGAAGAGCGCAGTAAAGATCCATTTTTGGAGCTCTATGATTGGACAGAGAAGTCAAATGGGAGCTTGTTCAAATAA

Protein sequence

MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSLGDRHLLPHLPADDSSMNFGDPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGDGVITTPPCDSPAVAVTGNSPTSNPLCLRDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHNNENLATVTIKEEDMNEDQTTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMNEHDRHHATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSNGSLFK
BLAST of Carg25395-RA vs. NCBI nr
Match: XP_022955061.1 (probable WRKY transcription factor 35 [Cucurbita moschata])

HSP 1 Score: 785.4 bits (2027), Expect = 8.8e-224
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0

Query: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSLGDRHLLPHLPADDSSMNFGD 60
           MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSLGDRHLLPHLPADDSSMNFGD
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSLGDRHLLPHLPADDSSMNFGD 60

Query: 61  PLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGDGVIT 120
           PLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGDGVIT
Sbjct: 61  PLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGDGVIT 120

Query: 121 TPPCDSPAVAVTGNSPTSNPLCLRDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANS 180
           TPPCDSPAVAVTGNSPTSNPLCLRDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANS
Sbjct: 121 TPPCDSPAVAVTGNSPTSNPLCLRDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANS 180

Query: 181 RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT 240
           RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT
Sbjct: 181 RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT 240

Query: 241 YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHNNENLATVTIKEEDMNEDQ 300
           YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHNNENLATVTIKEEDMNEDQ
Sbjct: 241 YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHNNENLATVTIKEEDMNEDQ 300

Query: 301 TTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMNEHDRHHATDTMNDNLLRFSNHGF 360
           TTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMNEHDRHHATDTMNDNLLRFSNHGF
Sbjct: 301 TTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMNEHDRHHATDTMNDNLLRFSNHGF 360

Query: 361 SKGNDEERSKDPFLELYDWTEKSNGSLFK 390
           SKGNDEERSKDPFLELYDWTEKSNGSLFK
Sbjct: 361 SKGNDEERSKDPFLELYDWTEKSNGSLFK 389

BLAST of Carg25395-RA vs. NCBI nr
Match: XP_022994821.1 (probable WRKY transcription factor 35 [Cucurbita maxima])

HSP 1 Score: 760.4 bits (1962), Expect = 3.0e-216
Identity = 376/389 (96.66%), Postives = 381/389 (97.94%), Query Frame = 0

Query: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSLGDRHLLPHLPADDSSMNFGD 60
           MFCNLFEMDNDNYQQAGDLTDVIRASSAA SAA SSEFSLGDRHLLPHLPADDSSMNFGD
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAANSAAQSSEFSLGDRHLLPHLPADDSSMNFGD 60

Query: 61  PLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGDGVIT 120
           PLLLPTSST+DPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGDGVIT
Sbjct: 61  PLLLPTSSTQDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGDGVIT 120

Query: 121 TPPCDSPAVAVTGNSPTSNPLCLRDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANS 180
           TPPCDSPAVA T NSP+SNPLCLRDNSDLQISPPRNPA+KRRKNQVKKVVCIPAPAPANS
Sbjct: 121 TPPCDSPAVAATRNSPSSNPLCLRDNSDLQISPPRNPAIKRRKNQVKKVVCIPAPAPANS 180

Query: 181 RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT 240
           RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT
Sbjct: 181 RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT 240

Query: 241 YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHNNENLATVTIKEEDMNEDQ 300
           YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHNNENLAT+ IKEEDMNEDQ
Sbjct: 241 YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHNNENLATMKIKEEDMNEDQ 300

Query: 301 TTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMNEHDRHHATDTMNDNLLRFSNHGF 360
           TTEKA TTITTNQHQVEGDHQEFLYDLIFDEF+DQMNEHD  HATDTMNDNLLRFSNHGF
Sbjct: 301 TTEKAATTITTNQHQVEGDHQEFLYDLIFDEFSDQMNEHDHRHATDTMNDNLLRFSNHGF 360

Query: 361 SKGNDEERSKDPFLELYDWTEKSNGSLFK 390
           SKGNDEERSKDPFLELYDWTEKSNGSLFK
Sbjct: 361 SKGNDEERSKDPFLELYDWTEKSNGSLFK 389

BLAST of Carg25395-RA vs. NCBI nr
Match: XP_023542883.1 (probable WRKY transcription factor 35 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 753.8 bits (1945), Expect = 2.8e-214
Identity = 375/389 (96.40%), Postives = 381/389 (97.94%), Query Frame = 0

Query: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSLGDRHLLPHLPADDSSMNFGD 60
           MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSLGDRHLLPHLP DDSSMNFGD
Sbjct: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSLGDRHLLPHLPTDDSSMNFGD 60

Query: 61  PLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGDGVIT 120
           PLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKV DGVIT
Sbjct: 61  PLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVVDGVIT 120

Query: 121 TPPCDSPAVAVTGNSPTSNPLCLRDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANS 180
           TPPCDSPAVA TGNSPTSNPLCLRDNSDLQISPPRNPA+KRRKNQVKKVVCIPAPAPANS
Sbjct: 121 TPPCDSPAVAATGNSPTSNPLCLRDNSDLQISPPRNPAIKRRKNQVKKVVCIPAPAPANS 180

Query: 181 RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT 240
           RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT
Sbjct: 181 RSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVIT 240

Query: 241 YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHEHNNENLATVTIKEEDMNEDQ 300
           YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHE+NNENLA++TIKEEDMNE+Q
Sbjct: 241 YTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHENNNENLASMTIKEEDMNEEQ 300

Query: 301 TTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMNEHDRHHATDTMNDNLLRFSNHGF 360
            TEKA   ITTNQHQVEGDHQEFLYDLIFDEFADQMNEH+RHHATDTMNDNLLRFSNHGF
Sbjct: 301 MTEKA--AITTNQHQVEGDHQEFLYDLIFDEFADQMNEHNRHHATDTMNDNLLRFSNHGF 360

Query: 361 SKGNDEERSKDPFLELYDWTEKSNGSLFK 390
           SKGNDEE SKDPFLELYDWTEKSNGSLFK
Sbjct: 361 SKGNDEECSKDPFLELYDWTEKSNGSLFK 387

BLAST of Carg25395-RA vs. NCBI nr
Match: XP_011659293.1 (PREDICTED: probable WRKY transcription factor 14 [Cucumis sativus] >KGN65962.1 hypothetical protein Csa_1G547510 [Cucumis sativus])

HSP 1 Score: 493.0 bits (1268), Expect = 9.1e-136
Identity = 281/418 (67.22%), Postives = 306/418 (73.21%), Query Frame = 0

Query: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSL----GDRHLLPHLPADDSSM 60
           MFC+LFEMDN+NYQQAGDLTDVIR SSA  +   SSEFSL    GDR L  HLPAD+SSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 60

Query: 61  NFGDPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGD 120
           NFGDPL  P   TRDPFL PHFS FNF S GGG G  A ++PS +   MLQISP+S  GD
Sbjct: 61  NFGDPLSFP---TRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSS--GD 120

Query: 121 GVITTPPCDSPAVAVTGNSPT----------------SNPLCLRDNSDLQISPPRNPALK 180
           GVI+TPPC+S A AV GNSP                 SN LCL +NS +QIS PRN A K
Sbjct: 121 GVISTPPCESLATAV-GNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANK 180

Query: 181 RRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240
           RRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG
Sbjct: 181 RRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240

Query: 241 CSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTT-RITSPKH 300
           CSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRT+T+TT   TSPKH
Sbjct: 241 CSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKH 300

Query: 301 EHNNENLATVTIKEEDMNEDQTTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMNEH 360
           E  N+ L T TIKEE+M+                    GDHQ+F YDLIF EFADQM   
Sbjct: 301 ERINDILPTTTIKEEEMD---XXXXXXXXXXXXXXXXXGDHQDFPYDLIFTEFADQMXXX 360

Query: 361 DRHH--------ATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSNGSLFK 390
                       + D +N+N+L FSNHGFS GN  E SKDPFLELYDW E S+GSLFK
Sbjct: 361 XXXXXXXXXXXLSADPLNNNMLMFSNHGFSAGNG-EGSKDPFLELYDWAENSSGSLFK 408

BLAST of Carg25395-RA vs. NCBI nr
Match: XP_008465657.1 (PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo])

HSP 1 Score: 444.9 bits (1143), Expect = 2.8e-121
Identity = 261/417 (62.59%), Postives = 288/417 (69.06%), Query Frame = 0

Query: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSL----GDRHLLPHLPADDSSM 60
           MFC+LFEMDN+NYQQAGDLTDVIR +SA  +   SSEFSL    G+  L  HLPAD+SSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAPVAGHFSSEFSLDSFSGEGRLWSHLPADNSSM 60

Query: 61  NFGDPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGD 120
           NFGDPL   +  TRDPFL PHFS FNF S GGG G  AA++PS +   MLQISP+S  GD
Sbjct: 61  NFGDPL---SFQTRDPFLLPHFSPFNFASDGGGEGL-AADQPSNLLSHMLQISPSS--GD 120

Query: 121 GVITTPPCDSPAVAVTGNSPT----------------SNPLCLRDNSDLQISPPRNPALK 180
           GVI+TPPC+S A AV GNSP+                SNPLCL +NS +QIS PRN A K
Sbjct: 121 GVISTPPCESLASAV-GNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNSANK 180

Query: 181 RRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240
           RRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG
Sbjct: 181 RRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240

Query: 241 CSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHE 300
           CSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR              
Sbjct: 241 CSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR-XXXXXXXXXXXXX 300

Query: 301 HNNENLATVTIKEEDMNEDQTTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMN--E 360
                                                GDHQ+F +DLIF EFADQMN  E
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDHQDFPFDLIFTEFADQMNDLE 360

Query: 361 HD------RHHATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSNGSLFK 390
           H+       H + D +N+N+L F +HGFS GN  E SKDPFLELYDW E S+GSLFK
Sbjct: 361 HENXXXXSHHLSADPLNNNMLMFGSHGFSTGNG-EGSKDPFLELYDWAENSSGSLFK 408

BLAST of Carg25395-RA vs. TAIR10
Match: AT1G30650.1 (WRKY DNA-binding protein 14)

HSP 1 Score: 219.2 bits (557), Expect = 4.6e-57
Identity = 152/300 (50.67%), Postives = 184/300 (61.33%), Query Frame = 0

Query: 3   CNLFE-MDNDNYQQAGDLTDVIRASSA---ATSAAHSSEFSLGDRHLLPHLPADDSSMNF 62
           C++ E +D +N+Q  GDLTDV+R       +     S+ + L   H  P  P+D +   F
Sbjct: 2   CSVSELLDMENFQ--GDLTDVVRGIGGHVLSPETPPSNIWPLPLSHPTPS-PSDLNINPF 61

Query: 63  GDPLLLPTSSTRDPFLHP--------HFSSF---NFDSHGGGWGFTA--------AERPS 122
           GDP +    S  DP L          +FS+    N + H    GF           +   
Sbjct: 62  GDPFV----SMDDPLLQELNSITNSGYFSTVGDNNNNIHNNN-GFLVPKVFEEDHIKSQC 121

Query: 123 TVFPRMLQISPTSKVGDGVITTPPCDSPAV------AVTGNSP-------TSNPL-CL-- 182
           ++FPR ++IS ++ + D    + PC+SPA+      A    SP       T++P  CL  
Sbjct: 122 SIFPR-IRISHSNIIHD----SSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLV 181

Query: 183 ---RDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQ 242
                +S +QIS PRN  LKRRK+Q KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQ
Sbjct: 182 DGTTFSSQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQ 241

Query: 243 KPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGST 261
           KPIKGSP+PRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWP QRNALAGST
Sbjct: 242 KPIKGSPFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGST 287

BLAST of Carg25395-RA vs. TAIR10
Match: AT2G34830.1 (WRKY DNA-binding protein 35)

HSP 1 Score: 214.9 bits (546), Expect = 8.6e-56
Identity = 142/293 (48.46%), Postives = 168/293 (57.34%), Query Frame = 0

Query: 11  DNYQQAGDLTDVIRA-SSAATSAAHSSEFSLGDRHLLPHLPADDSSMNF----------G 70
           DN+Q  GDLTDV+R   S   S +           + P  P  D  + F           
Sbjct: 2   DNFQ--GDLTDVVRGIGSGHVSPSPGPPEGXXXXSMSPP-PTSDLHVEFPSAATSASCLA 61

Query: 71  DPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQ---------ISP 130
           +P   P  S +DP +H   S  +      G G   + +   +FP++ +         + P
Sbjct: 62  NPFGDPFVSMKDPLIHLPASYIS------GAGDNKSNKSFAIFPKIFEDDHIKSQCSVFP 121

Query: 131 TSKV--GDGVITTPPCDSPAVAV----------------TGNSPTSNPLCLRDN-----S 190
             K+   + +     C+SPA+ V                T NSP +  L   +N     S
Sbjct: 122 RIKISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNXTSSCS 181

Query: 191 DLQI-SPPRNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGS 250
            +QI S PRN  +KRRK+Q KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPIKGS
Sbjct: 182 QVQISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPIKGS 241

Query: 251 PYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGS 260
           PYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGS
Sbjct: 242 PYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGS 284

BLAST of Carg25395-RA vs. TAIR10
Match: AT4G01250.1 (WRKY family transcription factor)

HSP 1 Score: 144.4 bits (363), Expect = 1.4e-34
Identity = 80/140 (57.14%), Postives = 93/140 (66.43%), Query Frame = 0

Query: 140 PLCLRDNSDLQISPP---RNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWR 199
           PL L  +S    S P        KRRK Q KKV  + A           E + SD+WAWR
Sbjct: 86  PLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAA-----------EALNSDVWAWR 145

Query: 200 KYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNAL 259
           KYGQKPIKGSPYPRGYYRCS+SKGC ARKQVER+R+DP M ++TYT+EHNHP PT RN+L
Sbjct: 146 KYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSL 205

Query: 260 AGSTRSHPSRTSTSTTRITS 277
           AGSTR  PS   TS +  T+
Sbjct: 206 AGSTRQKPSDQQTSKSPTTT 214

BLAST of Carg25395-RA vs. TAIR10
Match: AT5G45050.1 (Disease resistance protein (TIR-NBS-LRR class))

HSP 1 Score: 142.9 bits (359), Expect = 4.2e-34
Identity = 99/226 (43.81%), Postives = 122/226 (53.98%), Query Frame = 0

Query: 160  KRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 219
            +R+ N+ K+VVC+       SRS       SDLW WRKYGQKPIK SPYPR YYRC+SSK
Sbjct: 1160 RRKNNKEKRVVCV---VDRGSRS-------SDLWVWRKYGQKPIKSSPYPRSYYRCASSK 1219

Query: 220  GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITS--- 279
            GC ARKQVERSRTDPN+ VITY SEHNHP+PT RN LAGSTRS  S+ S  TT  +S   
Sbjct: 1220 GCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVS 1279

Query: 280  -----PKHEH-----NNENLATVTIKEEDMNEDQTTEKATTTITTNQHQVEGDHQEFLYD 339
                 P   H      +   A + +KEEDM                    + D+ EF  D
Sbjct: 1280 QDKEGPDKSHLPSSPASPPYAAMVVKEEDME-------------------QWDNMEFDVD 1339

Query: 340  LIFDEFADQMNEHDRHHATDTMNDN-LLRFSNHGFSKGNDEERSKD 372
            +  D F  ++   D     D + +N    F +   S GN E + K+
Sbjct: 1340 VEEDTFIPELFPEDTFADMDKLEENSQTMFLSRRSSGGNMEAQGKN 1356

BLAST of Carg25395-RA vs. TAIR10
Match: AT5G52830.1 (WRKY DNA-binding protein 27)

HSP 1 Score: 135.6 bits (340), Expect = 6.7e-32
Identity = 73/135 (54.07%), Postives = 87/135 (64.44%), Query Frame = 0

Query: 160 KRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 219
           ++RKNQ K+ +C            + E + SDLWAWRKYGQKPIKGSPYPR YYRCSSSK
Sbjct: 145 RKRKNQQKRTIC----------HVTQENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSK 204

Query: 220 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKH 279
           GC ARKQVERS  DPN+ ++TYT EH HP PT RN+LAGSTR+     +      TSP  
Sbjct: 205 GCLARKQVERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNKSQPVNPVPKPDTSPLS 260

Query: 280 EHNNENLATVTIKEE 295
           +         T+KEE
Sbjct: 265 D---------TVKEE 260

BLAST of Carg25395-RA vs. Swiss-Prot
Match: sp|Q9SA80|WRK14_ARATH (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 8.3e-56
Identity = 152/300 (50.67%), Postives = 184/300 (61.33%), Query Frame = 0

Query: 3   CNLFE-MDNDNYQQAGDLTDVIRASSA---ATSAAHSSEFSLGDRHLLPHLPADDSSMNF 62
           C++ E +D +N+Q  GDLTDV+R       +     S+ + L   H  P  P+D +   F
Sbjct: 2   CSVSELLDMENFQ--GDLTDVVRGIGGHVLSPETPPSNIWPLPLSHPTPS-PSDLNINPF 61

Query: 63  GDPLLLPTSSTRDPFLHP--------HFSSF---NFDSHGGGWGFTA--------AERPS 122
           GDP +    S  DP L          +FS+    N + H    GF           +   
Sbjct: 62  GDPFV----SMDDPLLQELNSITNSGYFSTVGDNNNNIHNNN-GFLVPKVFEEDHIKSQC 121

Query: 123 TVFPRMLQISPTSKVGDGVITTPPCDSPAV------AVTGNSP-------TSNPL-CL-- 182
           ++FPR ++IS ++ + D    + PC+SPA+      A    SP       T++P  CL  
Sbjct: 122 SIFPR-IRISHSNIIHD----SSPCNSPAMSAHVVAAAAAASPRGIINVDTNSPRNCLLV 181

Query: 183 ---RDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQ 242
                +S +QIS PRN  LKRRK+Q KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQ
Sbjct: 182 DGTTFSSQIQISSPRNLGLKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQ 241

Query: 243 KPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGST 261
           KPIKGSP+PRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWP QRNALAGST
Sbjct: 242 KPIKGSPFPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRNALAGST 287

BLAST of Carg25395-RA vs. Swiss-Prot
Match: sp|O64747|WRK35_ARATH (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.6e-54
Identity = 142/293 (48.46%), Postives = 168/293 (57.34%), Query Frame = 0

Query: 11  DNYQQAGDLTDVIRA-SSAATSAAHSSEFSLGDRHLLPHLPADDSSMNF----------G 70
           DN+Q  GDLTDV+R   S   S +           + P  P  D  + F           
Sbjct: 2   DNFQ--GDLTDVVRGIGSGHVSPSPGPPEGXXXXSMSPP-PTSDLHVEFPSAATSASCLA 61

Query: 71  DPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQ---------ISP 130
           +P   P  S +DP +H   S  +      G G   + +   +FP++ +         + P
Sbjct: 62  NPFGDPFVSMKDPLIHLPASYIS------GAGDNKSNKSFAIFPKIFEDDHIKSQCSVFP 121

Query: 131 TSKV--GDGVITTPPCDSPAVAV----------------TGNSPTSNPLCLRDN-----S 190
             K+   + +     C+SPA+ V                T NSP +  L   +N     S
Sbjct: 122 RIKISQSNNIHDASTCNSPAITVSSAAVAASPWGMINVNTTNSPRNCLLVDNNNXTSSCS 181

Query: 191 DLQI-SPPRNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGS 250
            +QI S PRN  +KRRK+Q KKVVCIPAPA  NSR SSGEVVPSDLWAWRKYGQKPIKGS
Sbjct: 182 QVQISSSPRNLGIKRRKSQAKKVVCIPAPAAMNSR-SSGEVVPSDLWAWRKYGQKPIKGS 241

Query: 251 PYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGS 260
           PYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGS
Sbjct: 242 PYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGS 284

BLAST of Carg25395-RA vs. Swiss-Prot
Match: sp|O04609|WRK22_ARATH (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.6e-33
Identity = 80/140 (57.14%), Postives = 93/140 (66.43%), Query Frame = 0

Query: 140 PLCLRDNSDLQISPP---RNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWR 199
           PL L  +S    S P        KRRK Q KKV  + A           E + SD+WAWR
Sbjct: 86  PLSLSASSGSVTSKPSGSNTSRSKRRKIQHKKVCHVAA-----------EALNSDVWAWR 145

Query: 200 KYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNAL 259
           KYGQKPIKGSPYPRGYYRCS+SKGC ARKQVER+R+DP M ++TYT+EHNHP PT RN+L
Sbjct: 146 KYGQKPIKGSPYPRGYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSL 205

Query: 260 AGSTRSHPSRTSTSTTRITS 277
           AGSTR  PS   TS +  T+
Sbjct: 206 AGSTRQKPSDQQTSKSPTTT 214

BLAST of Carg25395-RA vs. Swiss-Prot
Match: sp|Q9FL92|RRS1B_ARATH (Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 7.5e-33
Identity = 99/226 (43.81%), Postives = 122/226 (53.98%), Query Frame = 0

Query: 160  KRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 219
            +R+ N+ K+VVC+       SRS       SDLW WRKYGQKPIK SPYPR YYRC+SSK
Sbjct: 1160 RRKNNKEKRVVCV---VDRGSRS-------SDLWVWRKYGQKPIKSSPYPRSYYRCASSK 1219

Query: 220  GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITS--- 279
            GC ARKQVERSRTDPN+ VITY SEHNHP+PT RN LAGSTRS  S+ S  TT  +S   
Sbjct: 1220 GCFARKQVERSRTDPNVSVITYISEHNHPFPTLRNTLAGSTRSSSSKCSDVTTSASSTVS 1279

Query: 280  -----PKHEH-----NNENLATVTIKEEDMNEDQTTEKATTTITTNQHQVEGDHQEFLYD 339
                 P   H      +   A + +KEEDM                    + D+ EF  D
Sbjct: 1280 QDKEGPDKSHLPSSPASPPYAAMVVKEEDME-------------------QWDNMEFDVD 1339

Query: 340  LIFDEFADQMNEHDRHHATDTMNDN-LLRFSNHGFSKGNDEERSKD 372
            +  D F  ++   D     D + +N    F +   S GN E + K+
Sbjct: 1340 VEEDTFIPELFPEDTFADMDKLEENSQTMFLSRRSSGGNMEAQGKN 1356

BLAST of Carg25395-RA vs. Swiss-Prot
Match: sp|Q9FLX8|WRK27_ARATH (Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.2e-30
Identity = 73/135 (54.07%), Postives = 87/135 (64.44%), Query Frame = 0

Query: 160 KRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 219
           ++RKNQ K+ +C            + E + SDLWAWRKYGQKPIKGSPYPR YYRCSSSK
Sbjct: 145 RKRKNQQKRTIC----------HVTQENLSSDLWAWRKYGQKPIKGSPYPRNYYRCSSSK 204

Query: 220 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKH 279
           GC ARKQVERS  DPN+ ++TYT EH HP PT RN+LAGSTR+     +      TSP  
Sbjct: 205 GCLARKQVERSNLDPNIFIVTYTGEHTHPRPTHRNSLAGSTRNKSQPVNPVPKPDTSPLS 260

Query: 280 EHNNENLATVTIKEE 295
           +         T+KEE
Sbjct: 265 D---------TVKEE 260

BLAST of Carg25395-RA vs. TrEMBL
Match: tr|A0A0A0LYP6|A0A0A0LYP6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G547510 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 6.0e-136
Identity = 281/418 (67.22%), Postives = 306/418 (73.21%), Query Frame = 0

Query: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSL----GDRHLLPHLPADDSSM 60
           MFC+LFEMDN+NYQQAGDLTDVIR SSA  +   SSEFSL    GDR L  HLPAD+SSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRLSSAPVAGHFSSEFSLDPFSGDRQLWSHLPADNSSM 60

Query: 61  NFGDPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGD 120
           NFGDPL  P   TRDPFL PHFS FNF S GGG G  A ++PS +   MLQISP+S  GD
Sbjct: 61  NFGDPLSFP---TRDPFLLPHFSPFNFASDGGGEGLAADDQPSNLLSHMLQISPSS--GD 120

Query: 121 GVITTPPCDSPAVAVTGNSPT----------------SNPLCLRDNSDLQISPPRNPALK 180
           GVI+TPPC+S A AV GNSP                 SN LCL +NS +QIS PRN A K
Sbjct: 121 GVISTPPCESLATAV-GNSPRSTSVRGGGGLVLPTGGSNSLCLMENSGIQISSPRNSANK 180

Query: 181 RRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240
           RRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG
Sbjct: 181 RRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240

Query: 241 CSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTT-RITSPKH 300
           CSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRT+T+TT   TSPKH
Sbjct: 241 CSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTTTATTANKTSPKH 300

Query: 301 EHNNENLATVTIKEEDMNEDQTTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMNEH 360
           E  N+ L T TIKEE+M+                    GDHQ+F YDLIF EFADQM   
Sbjct: 301 ERINDILPTTTIKEEEMD---XXXXXXXXXXXXXXXXXGDHQDFPYDLIFTEFADQMXXX 360

Query: 361 DRHH--------ATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSNGSLFK 390
                       + D +N+N+L FSNHGFS GN  E SKDPFLELYDW E S+GSLFK
Sbjct: 361 XXXXXXXXXXXLSADPLNNNMLMFSNHGFSAGNG-EGSKDPFLELYDWAENSSGSLFK 408

BLAST of Carg25395-RA vs. TrEMBL
Match: tr|A0A1S3CQV3|A0A1S3CQV3_CUCME (probable WRKY transcription factor 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503297 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.9e-121
Identity = 261/417 (62.59%), Postives = 288/417 (69.06%), Query Frame = 0

Query: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSL----GDRHLLPHLPADDSSM 60
           MFC+LFEMDN+NYQQAGDLTDVIR +SA  +   SSEFSL    G+  L  HLPAD+SSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAPVAGHFSSEFSLDSFSGEGRLWSHLPADNSSM 60

Query: 61  NFGDPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGD 120
           NFGDPL   +  TRDPFL PHFS FNF S GGG G  AA++PS +   MLQISP+S  GD
Sbjct: 61  NFGDPL---SFQTRDPFLLPHFSPFNFASDGGGEGL-AADQPSNLLSHMLQISPSS--GD 120

Query: 121 GVITTPPCDSPAVAVTGNSPT----------------SNPLCLRDNSDLQISPPRNPALK 180
           GVI+TPPC+S A AV GNSP+                SNPLCL +NS +QIS PRN A K
Sbjct: 121 GVISTPPCESLASAV-GNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNSANK 180

Query: 181 RRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240
           RRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG
Sbjct: 181 RRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240

Query: 241 CSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHE 300
           CSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR              
Sbjct: 241 CSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR-XXXXXXXXXXXXX 300

Query: 301 HNNENLATVTIKEEDMNEDQTTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMN--E 360
                                                GDHQ+F +DLIF EFADQMN  E
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDHQDFPFDLIFTEFADQMNDLE 360

Query: 361 HD------RHHATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSNGSLFK 390
           H+       H + D +N+N+L F +HGFS GN  E SKDPFLELYDW E S+GSLFK
Sbjct: 361 HENXXXXSHHLSADPLNNNMLMFGSHGFSTGNG-EGSKDPFLELYDWAENSSGSLFK 408

BLAST of Carg25395-RA vs. TrEMBL
Match: tr|A0A1S3CPD8|A0A1S3CPD8_CUCME (probable WRKY transcription factor 14 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503297 PE=4 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 6.2e-117
Identity = 257/417 (61.63%), Postives = 284/417 (68.11%), Query Frame = 0

Query: 1   MFCNLFEMDNDNYQQAGDLTDVIRASSAATSAAHSSEFSL----GDRHLLPHLPADDSSM 60
           MFC+LFEMDN+NYQQAGDLTDVIR +SA  +   SSEFSL    G+  L  HLPAD+SSM
Sbjct: 1   MFCSLFEMDNNNYQQAGDLTDVIRPTSAPVAGHFSSEFSLDSFSGEGRLWSHLPADNSSM 60

Query: 61  NFGDPLLLPTSSTRDPFLHPHFSSFNFDSHGGGWGFTAAERPSTVFPRMLQISPTSKVGD 120
           NFGDPL   +  TRDPFL PHFS FNF S GGG G  AA++PS +   MLQISP+S  GD
Sbjct: 61  NFGDPL---SFQTRDPFLLPHFSPFNFASDGGGEGL-AADQPSNLLSHMLQISPSS--GD 120

Query: 121 GVITTPPCDSPAVAVTGNSPT----------------SNPLCLRDNSDLQISPPRNPALK 180
           GVI+TPPC+S A AV GNSP+                SNPLCL +NS +QIS PRN A K
Sbjct: 121 GVISTPPCESLASAV-GNSPSSTSVRGGGGVVVPTGGSNPLCLMENSGIQISSPRNSANK 180

Query: 181 RRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYPRGYYRCSSSKG 240
           RRK+QVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP    RCSSSKG
Sbjct: 181 RRKSQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRKYGQKPIKGSPYP----RCSSSKG 240

Query: 241 CSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSRTSTSTTRITSPKHE 300
           CSARKQVERSRT+PNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR              
Sbjct: 241 CSARKQVERSRTNPNMLVITYTSEHNHPWPTQRNALAGSTRSHPSR-XXXXXXXXXXXXX 300

Query: 301 HNNENLATVTIKEEDMNEDQTTEKATTTITTNQHQVEGDHQEFLYDLIFDEFADQMN--E 360
                                                GDHQ+F +DLIF EFADQMN  E
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDHQDFPFDLIFTEFADQMNDLE 360

Query: 361 HD------RHHATDTMNDNLLRFSNHGFSKGNDEERSKDPFLELYDWTEKSNGSLFK 390
           H+       H + D +N+N+L F +HGFS GN  E SKDPFLELYDW E S+GSLFK
Sbjct: 361 HENXXXXSHHLSADPLNNNMLMFGSHGFSTGNG-EGSKDPFLELYDWAENSSGSLFK 404

BLAST of Carg25395-RA vs. TrEMBL
Match: tr|A0A022PXY9|A0A022PXY9_ERYGU (Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a006007mg PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.0e-67
Identity = 166/307 (54.07%), Postives = 195/307 (63.52%), Query Frame = 0

Query: 3   CNLFEMDNDNYQQAGDLTDVIRASSAA-TSAAHSSEFSLGDRHL----------LPHLPA 62
           C+ F    +NYQQ GDLTD++RAS  + T+A  ++  +L D             + + P 
Sbjct: 2   CSHFLKRMENYQQ-GDLTDILRASGGSKTAAEEAAAAALADWQFPAANNTVDNNINNYPT 61

Query: 63  DDS--------SMNFGDPLLL----PTSSTRDP----FLHPHFSSFNFDSHGGGWGFTAA 122
           DD         S + GDPLLL      ++T  P    FLH   S    +S     GFT+ 
Sbjct: 62  DDPVDVFGDPFSSHMGDPLLLLHGINAAATAVPASGLFLHSS-SEETGNSSSSTVGFTSI 121

Query: 123 E---RPSTVFPRMLQISPTSKVGDGVITTPPCDSPAV----AVTGNSPTSNPLCLRDNS- 182
           +   RP+ +F RMLQI+P  K     +  PPCDSPA      +    P +N L +  NS 
Sbjct: 122 DGLKRPNNMFSRMLQIAPNPK-----LPLPPCDSPAAESPRGLVKAPPDNNTLIISPNSS 181

Query: 183 ----------DLQISPPRNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVPSDLWAWRK 242
                     +LQIS PRN  ++RRK+Q KKVVCIPAPAPANSR SSGE+VPSDLWAWRK
Sbjct: 182 KLGCLLESPGNLQISSPRNTGIRRRKSQAKKVVCIPAPAPANSR-SSGEIVPSDLWAWRK 241

Query: 243 YGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALA 265
           YGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALA
Sbjct: 242 YGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALA 300

BLAST of Carg25395-RA vs. TrEMBL
Match: tr|A0A2P4MH78|A0A2P4MH78_QUESU (Putative wrky transcription factor 14 OS=Quercus suber OX=58331 GN=CFP56_71155 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.2e-64
Identity = 162/322 (50.31%), Postives = 192/322 (59.63%), Query Frame = 0

Query: 11  DNYQQAGDLTDVIRASS------AATSAAHSSEFSLGDRHLLPHLPADDSSMNFGDPLLL 70
           DNYQ  GDLTD++RAS          S+   SE+      ++     +    NFGDP   
Sbjct: 5   DNYQ--GDLTDIVRASGGPYGCITGNSSQPISEWQFPSDPMIFSSIQEQPRDNFGDPF-- 64

Query: 71  PTSSTRDPFLHP-HFSSFNF-------------------DSH------------GGGWGF 130
             S+ RDP L+  H SS +                    +SH            GGG G 
Sbjct: 65  --SNMRDPLLNELHLSSSSSFFGSSNSADVIGTATSSVEESHTSGFTGLLHVDVGGGVGS 124

Query: 131 TAA------------ERPSTVFPRMLQISPTSKVGDGVITTPPCDSPAVAVTGNS----- 190
           +++            +RP  +F RMLQISP     +  ++  PC SP +A    +     
Sbjct: 125 SSSSTSVNVLDHDEMKRPCNIFSRMLQISP----NNAKLSVSPCGSPVMARGIKAPAMLP 184

Query: 191 ------PTSNPLCLRDNSDLQISPPRNPALKRRKNQVKKVVCIPAPAPANSRSSSGEVVP 250
                 P S+  C  DN+ +QIS PRNP +KRRK+Q KKVVCIPAPA ANSR  SGEVVP
Sbjct: 185 SDILSVPNSSKPCFIDNTAVQISSPRNPGIKRRKSQAKKVVCIPAPAAANSR-PSGEVVP 244

Query: 251 SDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPW 272
           SDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPW
Sbjct: 245 SDLWAWRKYGQKPIKGSPYPRGYYRCSSSKGCSARKQVERSRTDPNMLVITYTSEHNHPW 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022955061.18.8e-224100.00probable WRKY transcription factor 35 [Cucurbita moschata][more]
XP_022994821.13.0e-21696.66probable WRKY transcription factor 35 [Cucurbita maxima][more]
XP_023542883.12.8e-21496.40probable WRKY transcription factor 35 [Cucurbita pepo subsp. pepo][more]
XP_011659293.19.1e-13667.22PREDICTED: probable WRKY transcription factor 14 [Cucumis sativus] >KGN65962.1 h... [more]
XP_008465657.12.8e-12162.59PREDICTED: probable WRKY transcription factor 14 isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G30650.14.6e-5750.67WRKY DNA-binding protein 14[more]
AT2G34830.18.6e-5648.46WRKY DNA-binding protein 35[more]
AT4G01250.11.4e-3457.14WRKY family transcription factor[more]
AT5G45050.14.2e-3443.81Disease resistance protein (TIR-NBS-LRR class)[more]
AT5G52830.16.7e-3254.07WRKY DNA-binding protein 27[more]
Match NameE-valueIdentityDescription
sp|Q9SA80|WRK14_ARATH8.3e-5650.67Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
sp|O64747|WRK35_ARATH1.6e-5448.46Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
sp|O04609|WRK22_ARATH2.6e-3357.14WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1[more]
sp|Q9FL92|RRS1B_ARATH7.5e-3343.81Disease resistance protein RRS1B OS=Arabidopsis thaliana OX=3702 GN=RRS1B PE=1 S... [more]
sp|Q9FLX8|WRK27_ARATH1.2e-3054.07Probable WRKY transcription factor 27 OS=Arabidopsis thaliana OX=3702 GN=WRKY27 ... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LYP6|A0A0A0LYP6_CUCSA6.0e-13667.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G547510 PE=4 SV=1[more]
tr|A0A1S3CQV3|A0A1S3CQV3_CUCME1.9e-12162.59probable WRKY transcription factor 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
tr|A0A1S3CPD8|A0A1S3CPD8_CUCME6.2e-11761.63probable WRKY transcription factor 14 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
tr|A0A022PXY9|A0A022PXY9_ERYGU2.0e-6754.07Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a006007mg PE... [more]
tr|A0A2P4MH78|A0A2P4MH78_QUESU1.2e-6450.31Putative wrky transcription factor 14 OS=Quercus suber OX=58331 GN=CFP56_71155 P... [more]
The following terms have been associated with this mRNA:
Vocabulary: Molecular Function
TermDefinition
GO:0043565sequence-specific DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: INTERPRO
TermDefinition
IPR036576WRKY_dom_sf
IPR003657WRKY_dom
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg25395Carg25395gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg25395-RACarg25395-RA-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25395-RA.exon.1Carg25395-RA.exon.1exon
Carg25395-RA.exon.2Carg25395-RA.exon.2exon
Carg25395-RA.exon.3Carg25395-RA.exon.3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg25395-RA.CDS.1Carg25395-RA.CDS.1CDS
Carg25395-RA.CDS.2Carg25395-RA.CDS.2CDS
Carg25395-RA.CDS.3Carg25395-RA.CDS.3CDS


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 189..249
e-value: 1.4E-37
score: 140.9
IPR003657WRKY domainPFAMPF03106WRKYcoord: 191..248
e-value: 7.7E-26
score: 90.1
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 184..250
score: 33.109
IPR036576WRKY domain superfamilyGENE3DG3DSA:2.20.25.80coord: 176..251
e-value: 2.6E-29
score: 102.7
IPR036576WRKY domain superfamilySUPERFAMILYSSF118290WRKY DNA-binding domaincoord: 187..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..278
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..276
NoneNo IPR availablePANTHERPTHR32096FAMILY NOT NAMEDcoord: 13..312
NoneNo IPR availablePANTHERPTHR32096:SF49SUBFAMILY NOT NAMEDcoord: 13..312