Carg08181-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg08181-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Sentrin-specific protease 8
Location	Cucurbita_argyrosperma_scaffold_050 : 1167099 .. 1168055 (-)
Sequence length	831

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCTAAGTTCTTGACTTACAATGGCGTCGTGCTTCGAAGATCTGATGTACGCAGTTTAGAAGATTGTAACTATTTGAACGATGCCATCATTGATTTCTATTTTGCCTACCTGTTCGAGTCATATCAATTTGACGACATATTGTTAATACCGACGAGTATCTCGTTTTTGCTGGGCAACGCTTCTGACTGGGATACTTTCCTAGCTACAGTGGAATCTTTGAACTTTCATGAGAAGAAACTCATCTTCTTCACTGTCAACGACAACTCCAATTTCTCCTCTTCTGGCGGGTCTCATTGGAGCTTACTTGTGTACTGCAGAAAGCGAAATTTATTCATGCATTACCATAGCCTTCACATGATGAATAATGAATCTGCATTCGCCATCTACGAATCTGTCAATAGTTATATTAGTTATGATTCAGCTGAGTTCATGGAATGTCGAGCGCCTCGACAAAGGAACGAATATGATTGTGGATTGTACGTGATGGAAATTGCTAGAGTGATTTACGAGCACTATTTCAACTTTGATCAGTTTCAAAACGAAGATGAAATTTGTGGGTCGTTTAACGTGGTCCAAAGTGTGAATCGTAAACATGTTGAGTCCAATATGCGGTCTGAATCAATAAACATATATTTTTTTCAGTAGTTTTTTTTTTATTATCTGCCTCTTATTTTAGGATTGAATCAATAAACAGATCATTTTTTAAGTAATTTTGTTTATTATCTGCATTAATAAAGTCTTCACAAGATGAATAATGTCAACAATTATATCAGTTATGATTCAGCTGAGTTCATGGAATGCCGAGTGCCTGGACAAAGGAACGAATATGATCGTGGATTATTTAAATTTGATCATTTTCAAAACAAAGATGAAATTTGTGGGTCGTTTAAAGTGGTCCAAAATGTGAACTGTAAACATGTCGAGTCGAATATAGTGTCTGAAATGTTGTAA

mRNA sequence

ATGGATCCTAAGTTCTTGACTTACAATGGCGTCGTGCTTCGAAGATCTGATGTACGCAGTTTAGAAGATTGTAACTATTTGAACGATGCCATCATTGATTTCTATTTTGCCTACCTGTTCGAGTCATATCAATTTGACGACATATTGTTAATACCGACGAGTATCTCGTTTTTGCTGGGCAACGCTTCTGACTGGGATACTTTCCTAGCTACAGTGGAATCTTTGAACTTTCATGAGAAGAAACTCATCTTCTTCACTGTCAACGACAACTCCAATTTCTCCTCTTCTGGCGGGTCTCATTGGAGCTTACTTGTGTACTGCAGAAAGCGAAATTTATTCATGCATTACCATAGCCTTCACATGATGAATAATGAATCTGCATTCGCCATCTACGAATCTGTCAATAGTTATATTAGTTATGATTCAGCTGAGTTCATGGAATGTCGAGCGCCTCGACAAAGGAACGAATATGATTGTGGATTGTACGTGATGGAAATTGCTAGAGTGATTTACGAGCACTATTTCAACTTTGATCAGTTTCAAAACGAAGATGAAATTTGTGGGTCGTTTAACGTGGTCCAAAGTGTGAATCGTAAACATGTTGAGTCCAATATGCGTCTTCACAAGATGAATAATGTCAACAATTATATCAGTTATGATTCAGCTGAGTTCATGGAATGCCGAGTGCCTGGACAAAGGAACGAATATGATCGTGGATTATTTAAATTTGATCATTTTCAAAACAAAGATGAAATTTGTGGGTCGTTTAAAGTGGTCCAAAATGTGAACTGTAAACATGTCGAGTCGAATATAGTGTCTGAAATGTTGTAA

Coding sequence (CDS)

ATGGATCCTAAGTTCTTGACTTACAATGGCGTCGTGCTTCGAAGATCTGATGTACGCAGTTTAGAAGATTGTAACTATTTGAACGATGCCATCATTGATTTCTATTTTGCCTACCTGTTCGAGTCATATCAATTTGACGACATATTGTTAATACCGACGAGTATCTCGTTTTTGCTGGGCAACGCTTCTGACTGGGATACTTTCCTAGCTACAGTGGAATCTTTGAACTTTCATGAGAAGAAACTCATCTTCTTCACTGTCAACGACAACTCCAATTTCTCCTCTTCTGGCGGGTCTCATTGGAGCTTACTTGTGTACTGCAGAAAGCGAAATTTATTCATGCATTACCATAGCCTTCACATGATGAATAATGAATCTGCATTCGCCATCTACGAATCTGTCAATAGTTATATTAGTTATGATTCAGCTGAGTTCATGGAATGTCGAGCGCCTCGACAAAGGAACGAATATGATTGTGGATTGTACGTGATGGAAATTGCTAGAGTGATTTACGAGCACTATTTCAACTTTGATCAGTTTCAAAACGAAGATGAAATTTGTGGGTCGTTTAACGTGGTCCAAAGTGTGAATCGTAAACATGTTGAGTCCAATATGCGTCTTCACAAGATGAATAATGTCAACAATTATATCAGTTATGATTCAGCTGAGTTCATGGAATGCCGAGTGCCTGGACAAAGGAACGAATATGATCGTGGATTATTTAAATTTGATCATTTTCAAAACAAAGATGAAATTTGTGGGTCGTTTAAAGTGGTCCAAAATGTGAACTGTAAACATGTCGAGTCGAATATAGTGTCTGAAATGTTGTAA

Protein sequence

MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGNASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYHSLHMMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQFQNEDEICGSFNVVQSVNRKHVESNMRLHKMNNVNNYISYDSAEFMECRVPGQRNEYDRGLFKFDHFQNKDEICGSFKVVQNVNCKHVESNIVSEML

Homology

[Close]

BLAST of Carg08181-RA vs. NCBI nr
Match: XP_022942008.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 411.0 bits (1055), Expect = 3.2e-111
Identity = 202/211 (95.73%), Postives = 204/211 (96.68%), Query Frame = 0


Query: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLG 60
           MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQ DDILLI TSISFLLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLG 60

Query: 61  NASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYHSLH 120
           NASDWDTF+ATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHY SLH
Sbjct: 61  NASDWDTFVATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF 180
           MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF 180

Query: 181 QNEDEICGSFNVVQSVNRKHVESNMRLHKMN 212
           QNEDEICGSFNVVQSVN KHVESNMR   +N
Sbjct: 181 QNEDEICGSFNVVQSVNCKHVESNMRSESIN 211

[Close]

BLAST of Carg08181-RA vs. NCBI nr
Match: XP_022988745.1 (NEDD8-specific protease 1-like [Cucurbita maxima])

HSP 1 Score: 402.9 bits (1034), Expect = 8.7e-109
Identity = 197/206 (95.63%), Postives = 199/206 (96.60%), Query Frame = 0


Query: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLG 60
           MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQ DDILL+PTSISFLLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYHSLH 120
           NA DWDTF+ATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHY SLH
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF 180
           MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRN YDCGLYVMEIARVIYE YFNFDQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180

Query: 181 QNEDEICGSFNVVQSVNRKHVESNMR 207
           QNEDEICGSFNVVQ VN KHVESNMR
Sbjct: 181 QNEDEICGSFNVVQRVNCKHVESNMR 206

[Close]

BLAST of Carg08181-RA vs. NCBI nr
Match: XP_022953908.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 308.1 bits (788), Expect = 2.9e-80
Identity = 151/210 (71.90%), Postives = 171/210 (81.43%), Query Frame = 0


Query: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLG 60
           M  KFLTY  VV++  D+ SLE  NYLND IIDFYFAYL  +YQ ++IL IPTS+SFLLG
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYHSLH 120
           N  DWD F++T+ESLN HEKKLIFF VNDNSN SSSGGSHWSLLVYCRKRNLFMHY SL 
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF 180
            MN+E AF +YESV+ YI+YD AEFMECRAPRQ N YDCGLYVME ARVIYEHYFN DQF
Sbjct: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180

Query: 181 QNEDEICGSFNVVQSVNRKHVESNMRLHKM 211
           +++DEI G  NV++SV  K+VESNMR   M
Sbjct: 181 RSKDEISGPINVMRSVKSKYVESNMRSEMM 210

[Close]

BLAST of Carg08181-RA vs. NCBI nr
Match: XP_023526001.1 (NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 307.4 bits (786), Expect = 5.0e-80
Identity = 148/160 (92.50%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLG 60
           MDPKFLTYNGVVLRR DVRSLEDCNYLND IIDFYFAYLFES+Q DDILL+PTSISFLLG
Sbjct: 1   MDPKFLTYNGVVLRRFDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG 60

Query: 61  NASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYHSLH 120
           NA DWDTF+AT+ESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMH+ SLH
Sbjct: 61  NALDWDTFMATMESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHHDSLH 120

Query: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCG 161
           MMNNESAF IYESVNSYISYDSAEFMECRAPRQRN YDCG
Sbjct: 121 MMNNESAFTIYESVNSYISYDSAEFMECRAPRQRNGYDCG 160

[Close]

BLAST of Carg08181-RA vs. NCBI nr
Match: XP_023549404.1 (NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 301.6 bits (771), Expect = 2.7e-78
Identity = 148/206 (71.84%), Postives = 168/206 (81.55%), Query Frame = 0


Query: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLG 60
           M  KFLTY  VV++ SD+ SLE  NYLND IIDFYFAYL  +YQ ++IL IPTS+SFLLG
Sbjct: 1   MAAKFLTYGDVVIQGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYHSLH 120
           N  DWD F++T+ESLN  EKKLIFF VNDNSN SSSGGSHWSLLVYCRKRNLFMHY SL 
Sbjct: 61  NTLDWDIFISTIESLNLPEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF 180
            MN+ESAF +YESVN YI+Y+ AEFMECR PRQ N YDCGLYVME ARVIYEHY N DQF
Sbjct: 121 TMNSESAFTLYESVNGYINYEPAEFMECRTPRQNNGYDCGLYVMETARVIYEHYCNVDQF 180

Query: 181 QNEDEICGSFNVVQSVNRKHVESNMR 207
           +++DEI G  NV++SV  K+VE NMR
Sbjct: 181 RSKDEISGPINVMRSVKSKYVEFNMR 206

[Close]

BLAST of Carg08181-RA vs. TAIR10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 135.6 bits (340), Expect = 4.7e-32
Identity = 75/187 (40.11%), Postives = 114/187 (60.96%), Query Frame = 0


Query: 2   DPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGN 61
           D K L+Y  VVLRRSD+  L    +LND +I+FY ++L   +    I LIP SI+F + N
Sbjct: 7   DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66

Query: 62  ASDWDTFLATVESLNFHEKKLIFFTVNDNSNFS-SSGGSHWSLLVYCRKRNLFMHYHSLH 121
             D +     ++ LN  +K L+   VNDNSN   + GG HWSLLVY ++ N F+H+ S  
Sbjct: 67  CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126

Query: 122 MMNNESAFAIYESVNSYISYDSAEFMECR-APRQRNEYDCGLYVMEIARVIYEHYFNFDQ 181
            +N  SA  ++++V+ ++S   A + EC   P+Q+N YDCG++++  ARVI E +F+   
Sbjct: 127 GVNRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGG 186

Query: 182 FQNEDEI 187
            +N DE+
Sbjct: 187 MKNRDEL 192

[Close]

BLAST of Carg08181-RA vs. TAIR10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 42.7 bits (99), Expect = 4.2e-04
Identity = 30/131 (22.90%), Postives = 53/131 (40.46%), Query Frame = 0


Query: 9   NGVVLRRSDVRSLEDCNYLNDAIIDFYFAYL--------------FESYQFDDILLIPTS 68
           + VV+R+ D+  L+   ++ND IIDFY  YL              F  + F  +  +   
Sbjct: 303 DAVVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCFFFRKLANLDKG 362

Query: 69  ISFLLGNASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFM 126
                G    +       ++++  EK  IF  +N +         HWSL++ C    L  
Sbjct: 363 TPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCS--------FHWSLVIICHPGELVP 422

[Close]

BLAST of Carg08181-RA vs. Swiss-Prot
Match: sp|Q9LSS7|RUBP1_ARATH (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 8.5e-31
Identity = 75/187 (40.11%), Postives = 114/187 (60.96%), Query Frame = 0


Query: 2   DPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGN 61
           D K L+Y  VVLRRSD+  L    +LND +I+FY ++L   +    I LIP SI+F + N
Sbjct: 7   DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66

Query: 62  ASDWDTFLATVESLNFHEKKLIFFTVNDNSNFS-SSGGSHWSLLVYCRKRNLFMHYHSLH 121
             D +     ++ LN  +K L+   VNDNSN   + GG HWSLLVY ++ N F+H+ S  
Sbjct: 67  CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYM 126

Query: 122 MMNNESAFAIYESVNSYISYDSAEFMECR-APRQRNEYDCGLYVMEIARVIYEHYFNFDQ 181
            +N  SA  ++++V+ ++S   A + EC   P+Q+N YDCG++++  ARVI E +F+   
Sbjct: 127 GVNRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGG 186

Query: 182 FQNEDEI 187
            +N DE+
Sbjct: 187 MKNRDEL 192

[Close]

BLAST of Carg08181-RA vs. Swiss-Prot
Match: sp|Q5FVJ8|SENP8_RAT (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.2e-23
Identity = 67/181 (37.02%), Postives = 101/181 (55.80%), Query Frame = 0


Query: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQF----DDILLIPTSIS 60
           MDP  L+Y   +LR+SDV  L+  N+LND II F F Y F S QF    D +  I   ++
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEY-FASSQFHDCSDHVCFISPEVT 60

Query: 61  FLLGNASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHY 120
             +   S        +E L+   K+++F  +NDNSN  ++GG+HWSLLVY + +N F HY
Sbjct: 61  QFIKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHY 120

Query: 121 HSLHMMNNESAFAIYESVNSYISY--DSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHY 176
            S    N+  A  + E + +++    D   F+E +AP Q+N YDCG+YV+     + ++ 
Sbjct: 121 DSHSRSNSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQQNSYDCGMYVICNTEALCQNL 179

[Close]

BLAST of Carg08181-RA vs. Swiss-Prot
Match: sp|Q96LD8|SENP8_HUMAN (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.8e-23
Identity = 65/181 (35.91%), Postives = 103/181 (56.91%), Query Frame = 0


Query: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQF----DDILLIPTSIS 60
           MDP  L+Y   +LR+SDV  L+  ++LND II F F Y F + QF    D +  I   ++
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEY-FANSQFHDCSDHVSFISPEVT 60

Query: 61  FLLGNASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHY 120
             +   S+       +E L+   K+++F  +NDNSN  ++GG+HWSLLVY + +N F HY
Sbjct: 61  QFIKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHY 120

Query: 121 HSLHMMNNESAFAIYESVNSYISY--DSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHY 176
            S    N+  A  + E + +++    D   F+E +AP Q+N YDCG+YV+     + +++
Sbjct: 121 DSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNF 179

[Close]

BLAST of Carg08181-RA vs. Swiss-Prot
Match: sp|Q9D2Z4|SENP8_MOUSE (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 3.2e-22
Identity = 72/213 (33.80%), Postives = 109/213 (51.17%), Query Frame = 0


Query: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQF----DDILLIPTSIS 60
           MDP  L+Y   +LR+SDV  L+  ++LND II F F Y F + QF    D +  I   ++
Sbjct: 1   MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEY-FANSQFHDCSDHVCFISPEVT 60

Query: 61  FLLGNASDWDTFLATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHY 120
             +   S        +E L+   K+++F  +NDNSN  ++GG+HWSLLVY + +N F HY
Sbjct: 61  QFIKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHY 120

Query: 121 HSLHMMNNESAFAIYESVNSYISY--DSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHY 180
            S    N+  A  + E + +++    D   F+E +AP Q N YDCG+YV           
Sbjct: 121 DSHSRSNSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQENSYDCGMYV----------- 180

Query: 181 FNFDQFQNEDEICGSFNVVQSVNRKHVESNMRL 208
                      IC +  + QS+ R+  ES ++L
Sbjct: 181 -----------ICNTEALCQSLFRRQPESPLQL 189

[Close]

BLAST of Carg08181-RA vs. Swiss-Prot
Match: sp|Q54XR2|SENP8_DICDI (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.0e-15
Identity = 55/179 (30.73%), Postives = 96/179 (53.63%), Query Frame = 0


Query: 2   DPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQF------DDILLIPTSI 61
           DP  ++YN   + +SD+  L+   +LND+II FY  +L +  +       + +LL+  S+
Sbjct: 3   DPLIVSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLSPSV 62

Query: 62  SFLLGNA-SDWDTFLATVESLNFHEKKLIFFTVNDNSNFS-SSGGSHWSLLVYCRKRNLF 121
            F      S+ +  L   + L+   K++IFF + +N + +   GG+HWSLL++ +  N F
Sbjct: 63  VFCCSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSLNKF 122

Query: 122 MHYHSLHMMNNESAFAIYESVNSYIS-----YDSAEFM-ECRAPRQRNEYDCGLYVMEI 167
           ++Y S++  N+  A  I       +S      +  EF+   + P+Q+N YDCGLYV+ I
Sbjct: 123 IYYDSINSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVLSI 181

[Close]

BLAST of Carg08181-RA vs. TrEMBL
Match: tr|A0A2G5D630|A0A2G5D630_AQUCA (Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_02700117v1 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 6.1e-34
Identity = 86/179 (48.04%), Postives = 116/179 (64.80%), Query Frame = 0


Query: 4   KFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGNAS 63
           K L+YN VVLRRSD+  L   ++LND II+FYF+YL   Y  +DILL+P SISF + N  
Sbjct: 9   KILSYNDVVLRRSDLDILSGPHFLNDRIIEFYFSYLASCYASEDILLVPPSISFWISNCP 68

Query: 64  DWDTFLATVESLNFHEKKLIFFTVNDNSNFS-SSGGSHWSLLVYCRKRNLFMHYHSLHMM 123
           D ++    VE L F +KK++ F VN+N + S + GGSHWSLL Y RK+N+F+H+ SL   
Sbjct: 69  DIESLKDFVEPLKFPDKKMVIFPVNNNDDVSMAEGGSHWSLLAYERKKNVFVHHDSLASS 128

Query: 124 NNESAFAIYESVNSYI----SYDSAEFMEC-RAPRQRNEYDCGLYVMEIARVIYEHYFN 177
           N   A  +Y+ V  ++    S   A F++C  +P+Q N YDCGLYV  IA+VI E Y N
Sbjct: 129 NRCHAKRLYKGVVKFMGISDSKVDAPFIDCTTSPQQMNGYDCGLYVTAIAKVICEWYVN 187

[Close]

BLAST of Carg08181-RA vs. TrEMBL
Match: tr|A0A2G9G856|A0A2G9G856_9LAMI (Sentrin-specific cysteine protease (Ulp1 family) OS=Handroanthus impetiginosus OX=429701 GN=CDL12_26086 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.4e-33
Identity = 83/175 (47.43%), Postives = 113/175 (64.57%), Query Frame = 0


Query: 2   DPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGN 61
           D K L+YN VVLRRSD+  L    +LND II+FYF+YL   Y  +DILL+P SI+F +  
Sbjct: 24  DEKILSYNDVVLRRSDLDILSGPYFLNDRIIEFYFSYLTSCYSSEDILLVPPSIAFWIKE 83

Query: 62  ASDWDTFLATVESLNFHEKKLIFFTVNDNSNFS-SSGGSHWSLLVYCRKRNLFMHYHSLH 121
             D  +    VE L+  +KKLI F +NDN++ S + GGSHWSLL + R  N+F+H+ S  
Sbjct: 84  CPDVASLKDFVEPLHLSQKKLIIFPINDNADVSMAEGGSHWSLLAFERNANVFVHHDSSG 143

Query: 122 MMNNESAFAIYESVNSYISYDSAEFMECRA-PRQRNEYDCGLYVMEIARVIYEHY 175
            +N   A  +Y +V SYI+   A+++EC + P+Q N YDCGLYV  IAR I + Y
Sbjct: 144 GINGAHAKRVYRTVVSYIASSDAKYVECSSTPKQVNGYDCGLYVTAIARAICDWY 198

[Close]

BLAST of Carg08181-RA vs. TrEMBL
Match: tr|A0A1D1Z0K7|A0A1D1Z0K7_9ARAE (NEDD8-specific protease 1 OS=Anthurium amnicola OX=1678845 GN=NEDP1_1 PE=4 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 9.8e-32
Identity = 81/185 (43.78%), Postives = 114/185 (61.62%), Query Frame = 0


Query: 2   DPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGN 61
           D K L +  VVLRRSD+  L   +Y+ND II+F+F+ L  S   DDILL+P SISF + N
Sbjct: 7   DDKILCFRDVVLRRSDLDILRGPHYINDRIIEFFFSDLSSSVSLDDILLVPPSISFWIAN 66

Query: 62  ASDWDTFLATVESLNFHEKKLIFFTVNDNSNFS-SSGGSHWSLLVYCRKRNLFMHYHSLH 121
            SD++     VE L   EKK++ FTVNDN + + + GG HWSLL Y R++N+F+H+ S+ 
Sbjct: 67  CSDFEGLEDVVEPLKLSEKKVVVFTVNDNDDVTVAEGGLHWSLLAYDREKNVFIHHDSMA 126

Query: 122 MMNNESAFAIYESVNSYIS--YDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFD 181
            +N   A  +Y+ V  ++     SA F+E   PRQ N YDCGL+VM IA++I   +    
Sbjct: 127 GLNRLYADRLYKRVRRFLGDLGSSAGFVEGFTPRQENGYDCGLFVMLIAKLIVRWHVQRT 186

Query: 182 QFQNE 184
              N+
Sbjct: 187 DLDNQ 191

[Close]

BLAST of Carg08181-RA vs. TrEMBL
Match: tr|M4CR35|M4CR35_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis OX=51351 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.8e-31
Identity = 79/187 (42.25%), Postives = 117/187 (62.57%), Query Frame = 0


Query: 2   DPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGN 61
           D K L+YN VVLRRSD+  L   N+LND +I+FY +YL   +    I LIP SI+F + N
Sbjct: 7   DDKILSYNDVVLRRSDLDILNGPNFLNDRVIEFYLSYLSTVHTSSAISLIPPSIAFWISN 66

Query: 62  ASDWDTFLATVESLNFHEKKLIFFTVNDNSNFS-SSGGSHWSLLVYCRKRNLFMHYHSLH 121
             D ++    ++ L   +  L+ F VNDN+N   + GG HW+LLVY ++ N F H+ SL 
Sbjct: 67  CPDTESLKDFIKPLGLRDSDLLIFPVNDNTNVELAEGGLHWTLLVYYKEANSFFHHDSLM 126

Query: 122 MMNNESAFAIYESVNSYISYDSAEFMECR-APRQRNEYDCGLYVMEIARVIYEHYFNFDQ 181
            +N  SA  ++E+V+ ++S   A + EC   P+QRN YDCG+Y++ IA+VI + +F+   
Sbjct: 127 GLNKWSAKQLFEAVSPFVSDGDAIYRECSDTPQQRNGYDCGVYLLAIAQVICQ-WFSSGG 186

Query: 182 FQNEDEI 187
            QN DE+
Sbjct: 187 VQNRDEL 192

[Close]

BLAST of Carg08181-RA vs. TrEMBL
Match: tr|A0A151TN41|A0A151TN41_CAJCA (Sentrin-specific protease 8 OS=Cajanus cajan OX=3821 GN=KK1_022089 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.8e-31
Identity = 89/211 (42.18%), Postives = 120/211 (56.87%), Query Frame = 0


Query: 2   DPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQFDDILLIPTSISFLLGN 61
           D K L+YN VVLRRSD+  L    YLND II+FYF+YL   +   D LL+P SI+F +  
Sbjct: 7   DEKILSYNDVVLRRSDLDILSGPYYLNDRIIEFYFSYLSSCHPSQDTLLVPPSIAFWILK 66

Query: 62  ASDWDTFLATVESLNFHEKKLIFFTVNDNSNFS-SSGGSHWSLLVYCRKRNLFMHYHSLH 121
               +     +E L+  +K L+ F +NDN +   + GGSHWSLL Y R  NLF+H+ S  
Sbjct: 67  CPVAEALSDFIEPLHLSDKTLVIFPINDNDDVERAEGGSHWSLLAYYRNANLFVHHDSCR 126

Query: 122 MMNNESAFAIYESVNSYISYDS----AEFMECR-APRQRNEYDCGLYVMEIARVIYEHYF 181
            MN   A  +Y++V+ Y+   S    A FMEC  +P Q N YDCGLYV  IAR+I   + 
Sbjct: 127 SMNAAPAKQLYKAVSGYMGLSSSASEASFMECTDSPLQENGYDCGLYVTAIARIICSWHV 186

Query: 182 NFDQFQNEDEICGSFNVVQSVNRKHVESNMR 207
           N    +N D     F+VV+      V ++MR
Sbjct: 187 N---HKNTDTNDLWFSVVKEQVSPSVVASMR 214

The following BLAST results are available for this feature:

BLAST of Carg08181-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022942008.1	3.2e-111	95.73	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
XP_022988745.1	8.7e-109	95.63	NEDD8-specific protease 1-like [Cucurbita maxima]	[more]
XP_022953908.1	2.9e-80	71.90	NEDD8-specific protease 1-like [Cucurbita moschata]	[more]
XP_023526001.1	5.0e-80	92.50	NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023549404.1	2.7e-78	71.84	NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg08181-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT5G60190.1	4.7e-32	40.11	Cysteine proteinases superfamily protein	[more]
AT4G33620.1	4.2e-04	22.90	Cysteine proteinases superfamily protein	[more]

BLAST of Carg08181-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LSS7\|RUBP1_ARATH	8.5e-31	40.11	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
sp\|Q5FVJ8\|SENP8_RAT	2.2e-23	37.02	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]
sp\|Q96LD8\|SENP8_HUMAN	3.8e-23	35.91	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
sp\|Q9D2Z4\|SENP8_MOUSE	3.2e-22	33.80	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
sp\|Q54XR2\|SENP8_DICDI	1.0e-15	30.73	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]

BLAST of Carg08181-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A2G5D630\|A0A2G5D630_AQUCA	6.1e-34	48.04	Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_02700117v1 PE=4...	[more]
tr\|A0A2G9G856\|A0A2G9G856_9LAMI	1.4e-33	47.43	Sentrin-specific cysteine protease (Ulp1 family) OS=Handroanthus impetiginosus O...	[more]
tr\|A0A1D1Z0K7\|A0A1D1Z0K7_9ARAE	9.8e-32	43.78	NEDD8-specific protease 1 OS=Anthurium amnicola OX=1678845 GN=NEDP1_1 PE=4 SV=1	[more]
tr\|M4CR35\|M4CR35_BRARP	2.8e-31	42.25	Uncharacterized protein OS=Brassica rapa subsp. pekinensis OX=51351 PE=4 SV=1	[more]
tr\|A0A151TN41\|A0A151TN41_CAJCA	2.8e-31	42.18	Sentrin-specific protease 8 OS=Cajanus cajan OX=3821 GN=KK1_022089 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Molecular Function**
Term	Definition
GO:0008234	cysteine-type peptidase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR038765	Papain_like_cys_pep_sf
IPR003653	Peptidase_C48_C

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008234	cysteine-type peptidase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg08181	Carg08181	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08181-RA.CDS.2	Carg08181-RA.CDS.2	CDS
Carg08181-RA.CDS.1	Carg08181-RA.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg08181-RA.exon.2	Carg08181-RA.exon.2	exon
Carg08181-RA.exon.1	Carg08181-RA.exon.1	exon

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg08181-RA	Carg08181-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.40.395.10		coord: 1..188 e-value: 1.4E-51 score: 177.2
None	No IPR available	PANTHER	PTHR12606:SF90	SUBFAMILY NOT NAMED	coord: 8..143
None	No IPR available	PANTHER	PTHR12606	SENTRIN/SUMO-SPECIFIC PROTEASE	coord: 8..143
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 73..182 e-value: 5.8E-10 score: 39.3
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 11..170 score: 15.922
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	SSF54001	Cysteine proteinases	coord: 1..179