Carg03404-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg03404-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	ethylene-responsive transcription factor ERF069-like
Location	Cucurbita_argyrosperma_scaffold_005 : 1675621 .. 1676388 (+)
Sequence length	768

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGCGGCGGACATGGCTTCTTTGTCGGGGGACAGAGCTAACTGCCGCCGGAAACCGGCTGCTTCCGGGGACTCTGCAAAGCTGGAGCAAAAGCTTGTAAGGATCATCGTCACAGACGCCGACGCCACCGATTCGTCCAGTGAAGATGAATTGATTTTGGGATCAAGAACGGGAGTTAGAAGACAAGTAAGAGAGATCACAATTAATCGTTACTCTGTTCTTGATGGTTCTTCAGCGGAGTCCCCTGTTTCGGAGATCAGCAAGAAGCGGAATCGTAGATCACGTAGGTCCAAATCATCTTGCCGGAAGAAGTTCAGAGGAGTCAGACAGAGGGCTTGGGGCCGTTGGGCGGCGGAAATCCGGGATCCGATTCGCCGAAAGCGGATATGGCTTGGAACCTTTGATACGGCGGAAGAGGCGGCGGCGGTTTACGACCGAGCTGCTTTGGAACTCCAGGGCCCAAACGCCATAACGAACTTCTCCAGTGATGGGGCAGTTACAACGGCGGCCGACGGGGGTAACAAGCAGGAGGACGGCTTAGACTCACCCAAAACGACGGCGGTTTGGTCGCCGGCGTCAGTACTCCATTACGACGGCGTGTTTACTCCGATAGATGAGATGCTTTATTGTGGAGAGATCGATGAGTTCGAATTTGCCATGGCGACGGCTAGCTTGCCGGCGGCGCGGAGGCAGTACGGCGGCGAAGAAGATTTCGGGGAGTTGGAGCTGGACTTGGACTATTTCTTGGTTGACGTCATTTACTAA

mRNA sequence

ATGTCGGCGGCGGACATGGCTTCTTTGTCGGGGGACAGAGCTAACTGCCGCCGGAAACCGGCTGCTTCCGGGGACTCTGCAAAGCTGGAGCAAAAGCTTGTAAGGATCATCGTCACAGACGCCGACGCCACCGATTCGTCCAGTGAAGATGAATTGATTTTGGGATCAAGAACGGGAGTTAGAAGACAAGTAAGAGAGATCACAATTAATCGTTACTCTGTTCTTGATGGTTCTTCAGCGGAGTCCCCTGTTTCGGAGATCAGCAAGAAGCGGAATCGTAGATCACGTAGGTCCAAATCATCTTGCCGGAAGAAGTTCAGAGGAGTCAGACAGAGGGCTTGGGGCCGTTGGGCGGCGGAAATCCGGGATCCGATTCGCCGAAAGCGGATATGGCTTGGAACCTTTGATACGGCGGAAGAGGCGGCGGCGGTTTACGACCGAGCTGCTTTGGAACTCCAGGGCCCAAACGCCATAACGAACTTCTCCAGTGATGGGGCAGTTACAACGGCGGCCGACGGGGGTAACAAGCAGGAGGACGGCTTAGACTCACCCAAAACGACGGCGGTTTGGTCGCCGGCGTCAGTACTCCATTACGACGGCGTGTTTACTCCGATAGATGAGATGCTTTATTGTGGAGAGATCGATGAGTTCGAATTTGCCATGGCGACGGCTAGCTTGCCGGCGGCGCGGAGGCAGTACGGCGGCGAAGAAGATTTCGGGGAGTTGGAGCTGGACTTGGACTATTTCTTGGTTGACGTCATTTACTAA

Coding sequence (CDS)

ATGTCGGCGGCGGACATGGCTTCTTTGTCGGGGGACAGAGCTAACTGCCGCCGGAAACCGGCTGCTTCCGGGGACTCTGCAAAGCTGGAGCAAAAGCTTGTAAGGATCATCGTCACAGACGCCGACGCCACCGATTCGTCCAGTGAAGATGAATTGATTTTGGGATCAAGAACGGGAGTTAGAAGACAAGTAAGAGAGATCACAATTAATCGTTACTCTGTTCTTGATGGTTCTTCAGCGGAGTCCCCTGTTTCGGAGATCAGCAAGAAGCGGAATCGTAGATCACGTAGGTCCAAATCATCTTGCCGGAAGAAGTTCAGAGGAGTCAGACAGAGGGCTTGGGGCCGTTGGGCGGCGGAAATCCGGGATCCGATTCGCCGAAAGCGGATATGGCTTGGAACCTTTGATACGGCGGAAGAGGCGGCGGCGGTTTACGACCGAGCTGCTTTGGAACTCCAGGGCCCAAACGCCATAACGAACTTCTCCAGTGATGGGGCAGTTACAACGGCGGCCGACGGGGGTAACAAGCAGGAGGACGGCTTAGACTCACCCAAAACGACGGCGGTTTGGTCGCCGGCGTCAGTACTCCATTACGACGGCGTGTTTACTCCGATAGATGAGATGCTTTATTGTGGAGAGATCGATGAGTTCGAATTTGCCATGGCGACGGCTAGCTTGCCGGCGGCGCGGAGGCAGTACGGCGGCGAAGAAGATTTCGGGGAGTTGGAGCTGGACTTGGACTATTTCTTGGTTGACGTCATTTACTAA

Protein sequence

MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGVRRQVREITINRYSVLDGSSAESPVSEISKKRNRRSRRSKSSCRKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDGLDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFGELELDLDYFLVDVIY

Homology

[Close]

BLAST of Carg03404-RA vs. NCBI nr
Match: XP_022944036.1 (ethylene-responsive transcription factor ERF069-like [Cucurbita moschata])

HSP 1 Score: 458.0 bits (1177), Expect = 2.1e-125
Identity = 254/255 (99.61%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60
           MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV
Sbjct: 1   MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60

Query: 61  RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAE 120
           RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAE
Sbjct: 61  RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAE 120

Query: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG 180
           IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG
Sbjct: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG 180

Query: 181 LDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG 240
           LDSPKTTAVWSPASVL+YDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG
Sbjct: 181 LDSPKTTAVWSPASVLYYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG 240

Query: 241 ELELDLDYFLVDVIY 256
           ELELDLDYFLVDVIY
Sbjct: 241 ELELDLDYFLVDVIY 255

[Close]

BLAST of Carg03404-RA vs. NCBI nr
Match: XP_023512530.1 (ethylene-responsive transcription factor ERF069-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.4 bits (1165), Expect = 5.2e-124
Identity = 234/255 (91.76%), Postives = 234/255 (91.76%), Query Frame = 0


Query: 1   MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60
           MSAAD ASLSGDRANCRRKP ASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV
Sbjct: 1   MSAADRASLSGDRANCRRKPDASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60

Query: 61  RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAE 120
           RRQVREITINRYSVLDGSSAESP                  CRKKFRGVRQRAWGRWAAE
Sbjct: 61  RRQVREITINRYSVLDGSSAESPVSEISKKRNPRSRRSKSSCRKKFRGVRQRAWGRWAAE 120

Query: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG 180
           IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSD AVTTAADGGNKQEDG
Sbjct: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDAAVTTAADGGNKQEDG 180

Query: 181 LDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG 240
           LDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG
Sbjct: 181 LDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG 240

Query: 241 ELELDLDYFLVDVIY 256
           ELELDLDYFLVDVIY
Sbjct: 241 ELELDLDYFLVDVIY 255

[Close]

BLAST of Carg03404-RA vs. NCBI nr
Match: XP_022986928.1 (ethylene-responsive transcription factor ERF069-like [Cucurbita maxima])

HSP 1 Score: 443.0 bits (1138), Expect = 7.0e-121
Identity = 229/255 (89.80%), Postives = 232/255 (90.98%), Query Frame = 0


Query: 1   MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60
           MSAADMASLSGDRANCR KPA SGDSAKLEQKLVRIIVTDADAT+SSSEDELILGSRT V
Sbjct: 1   MSAADMASLSGDRANCRWKPATSGDSAKLEQKLVRIIVTDADATNSSSEDELILGSRTAV 60

Query: 61  RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAE 120
           RRQVREITINRYSVLDGSSAESP                  CRKKFRGVRQRAWGRWAAE
Sbjct: 61  RRQVREITINRYSVLDGSSAESPVSEISKKRNPRSRRSKSSCRKKFRGVRQRAWGRWAAE 120

Query: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG 180
           IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG
Sbjct: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG 180

Query: 181 LDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG 240
           L+SP+TTAVWSPASVLHYDGVFTP DEMLYCGEIDEF FAMATASLPAARRQYGGEEDFG
Sbjct: 181 LNSPRTTAVWSPASVLHYDGVFTPKDEMLYCGEIDEFGFAMATASLPAARRQYGGEEDFG 240

Query: 241 ELELDLDYFLVDVIY 256
           ELELDLDYFLVDVIY
Sbjct: 241 ELELDLDYFLVDVIY 255

[Close]

BLAST of Carg03404-RA vs. NCBI nr
Match: XP_016901953.1 (PREDICTED: ethylene-responsive transcription factor ERF069-like [Cucumis melo])

HSP 1 Score: 347.8 bits (891), Expect = 3.1e-92
Identity = 180/255 (70.59%), Postives = 196/255 (76.86%), Query Frame = 0


Query: 1   MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60
           MS  DMASLS  + NCRRK   SGDS K +Q+L+RIIVTDADATDSSSEDELILGSRT +
Sbjct: 1   MSVGDMASLSEHKTNCRRKALTSGDSTKPKQRLLRIIVTDADATDSSSEDELILGSRTTI 60

Query: 61  RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAE 120
           RRQVREITI R+SV D S                        R KFRGVRQR WGRWAAE
Sbjct: 61  RRQVREITIKRHSVADSSPKSPVSEICKKRNPRSRRSNNSSRRNKFRGVRQRPWGRWAAE 120

Query: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG 180
           +RDPI RKRIWLGTFDTAEEAAAVYDRAA+ELQGPNA TNFS DGAV TA DGG+K+EDG
Sbjct: 121 VRDPILRKRIWLGTFDTAEEAAAVYDRAAIELQGPNAATNFSGDGAVKTAVDGGSKKEDG 180

Query: 181 LDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG 240
           ++S KTTA WSP SVLHYD   TPI+EM+YCGE+DE    MA ASLPAARRQ  GEED G
Sbjct: 181 VESRKTTAAWSPTSVLHYDSFLTPIEEMVYCGEVDELGLEMAAASLPAARRQCCGEEDLG 240

Query: 241 ELELDLDYFLVDVIY 256
           E+ELDLDYFLVDVIY
Sbjct: 241 EMELDLDYFLVDVIY 255

[Close]

BLAST of Carg03404-RA vs. NCBI nr
Match: XP_022947817.1 (pathogenesis-related genes transcriptional activator PTI6-like [Cucurbita moschata])

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 180/255 (70.59%), Postives = 195/255 (76.47%), Query Frame = 0


Query: 1   MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60
           MS  +M      R N RRKP +SGDS +L+QKLVRIIVTDADATDSSS+DE++ GS  G+
Sbjct: 1   MSVGEMGFFPERRINHRRKPPSSGDSLELQQKLVRIIVTDADATDSSSDDEMVSGSTKGI 60

Query: 61  RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAE 120
           RRQVREI I  YSV  GSS ESP                   RKKFRGVRQR WGRWAAE
Sbjct: 61  RRQVREIPIGCYSVFYGSS-ESPVPAISKKRSTRSQSSKSFHRKKFRGVRQRPWGRWAAE 120

Query: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG 180
           IRDP RRKRIWLGTF+TAEEAAAVYDRAAL+L GPNA TNF  +G V+TAA GGNKQEDG
Sbjct: 121 IRDPTRRKRIWLGTFNTAEEAAAVYDRAALKLHGPNAETNFPDEGEVSTAAKGGNKQEDG 180

Query: 181 LDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAM-ATASLPAARRQYGGEEDF 240
           L SPKTTAVWSPASVLHYDG    IDEM +C EIDEFEF M A A LPAARRQ GGEEDF
Sbjct: 181 LKSPKTTAVWSPASVLHYDGALPLIDEM-FCREIDEFEFNMEAVARLPAARRQCGGEEDF 240

Query: 241 GELELDLDYFLVDVI 255
           GE+ELDLDYFLVDV+
Sbjct: 241 GEVELDLDYFLVDVL 253

[Close]

BLAST of Carg03404-RA vs. TAIR10
Match: AT4G27950.1 (cytokinin response factor 4)

HSP 1 Score: 125.6 bits (314), Expect = 4.5e-29
Identity = 68/140 (48.57%), Postives = 89/140 (63.57%), Query Frame = 0


Query: 26  SAKLEQKLVRIIVTDADATDSSSEDELILGSRTGVRRQVREITINRYSVLDGSSAESPXX 85
           S +   +LVR+ +TD DATDSSS++E  L  R  V+R + EI +   S   G  + SP  
Sbjct: 35  SVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVKRLINEIRVEPSSSSTGDVSASPTK 94

Query: 86  XXXXXXXXXXXXXXXXC---RKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEAA 145
                               +KK+RGVRQR WG+WAAEIRDP +R+RIWLGTF TAEEAA
Sbjct: 95  DRKRINVDSTVQKPSVSGQNQKKYRGVRQRPWGKWAAEIRDPEQRRRIWLGTFATAEEAA 154

Query: 146 AVYDRAALELQGPNAITNFS 163
            VYD AA++L+GP+A+TNF+
Sbjct: 155 IVYDNAAIKLRGPDALTNFT 174

[Close]

BLAST of Carg03404-RA vs. TAIR10
Match: AT5G53290.1 (cytokinin response factor 3)

HSP 1 Score: 115.5 bits (288), Expect = 4.7e-26
Identity = 71/145 (48.97%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 32  KLVRIIVTDADATDSSS--EDELILGSRTGVRRQVREITI--NRYSVLDG---------- 91
           K V I  TD DATDSSS  ++E  L  R  V+R V EIT+  +  +V+ G          
Sbjct: 37  KKVSICYTDPDATDSSSDEDEEDFLFPRRRVKRFVNEITVEPSCNNVVTGVSMKDRKRLS 96

Query: 92  SSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDT 151
           SS++                      KKFRGVRQR WG+WAAEIRDP +R+RIWLGTF+T
Sbjct: 97  SSSDETQSPASSRQRPNNKVSVSGQIKKFRGVRQRPWGKWAAEIRDPEQRRRIWLGTFET 156

Query: 152 AEEAAAVYDRAALELQGPNAITNFS 163
           AEEAA VYD AA+ L+GP+A+TNFS
Sbjct: 157 AEEAAVVYDNAAIRLRGPDALTNFS 181

[Close]

BLAST of Carg03404-RA vs. TAIR10
Match: AT4G23750.1 (cytokinin response factor 2)

HSP 1 Score: 102.4 bits (254), Expect = 4.1e-22
Identity = 58/146 (39.73%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 30  EQKLVRIIVTDADATD-----SSSEDELILGSRTGVRRQVREITINRYSVLDGSSAESPX 89
           + +++RI VTD DATD                    ++ V E+ ++  +V+ GS  +   
Sbjct: 33  QTRILRISVTDPDATDXXXXXXXXXXXXXXXXXXXXKKFVNEVYLDSGAVVTGSCGQMES 92

Query: 90  XXXXXXXXXXXXXXXXXC--------RKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFD 149
                                      KKFRGVRQR WG+WAAEIRDP++R R+WLGT++
Sbjct: 93  KKRQKRAVKSESTVSPVVSAXXXXTGEKKFRGVRQRPWGKWAAEIRDPLKRVRLWLGTYN 152

Query: 150 TAEEAAAVYDRAALELQGPNAITNFS 163
           TAEEAA VYD AA++L+GP+A+TNFS
Sbjct: 153 TAEEAAMVYDNAAIQLRGPDALTNFS 178

[Close]

BLAST of Carg03404-RA vs. TAIR10
Match: AT4G11140.1 (cytokinin response factor 1)

HSP 1 Score: 100.1 bits (248), Expect = 2.0e-21
Identity = 45/58 (77.59%), Postives = 51/58 (87.93%), Query Frame = 0


Query: 104 KKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNF 162
           +KFRGVRQR WG+WAAEIRDP RR R+WLGTFDTAEEAA VYD AA++L+GPNA  NF
Sbjct: 85  RKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVYDNAAIQLRGPNAELNF 142

[Close]

BLAST of Carg03404-RA vs. TAIR10
Match: AT3G61630.1 (cytokinin response factor 6)

HSP 1 Score: 95.5 bits (236), Expect = 5.0e-20
Identity = 78/170 (45.88%), Postives = 93/170 (54.71%), Query Frame = 0


Query: 32  KLVRIIVTDADATDSSSEDELILGSRTG--VRRQVREITINRYSVLDGSSAESPXXXXXX 91
           +LVRI VTD  ATD                V+R V+EI   +            XXXXXX
Sbjct: 29  RLVRITVTDPFATDXXXXXXXXXXXXXXPRVKRYVKEIRFCQGESXXXXXXXXXXXXXXX 88

Query: 92  XXXXXXXXXXXXCRKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEAAAVYDRAA 151
           XXXXXXX       KK+RGVRQR WG++AAEIRDP  R RIWLGTF TAEEAA  YDRAA
Sbjct: 89  XXXXXXXVSTSVKPKKYRGVRQRPWGKFAAEIRDPSSRTRIWLGTFVTAEEAAIAYDRAA 148

Query: 152 LELQGPNAITNFSSDGAVTTAADGGNKQEDGLDSPKTTAVWSPASVLHYD 200
           + L+GP A+TNF +    T   D               ++ SP SVL ++
Sbjct: 149 IHLKGPKALTNFLTPPTPTPVIDLQTVSACDYGRDSRQSLHSPTSVLRFN 198

[Close]

BLAST of Carg03404-RA vs. Swiss-Prot
Match: sp|O04682|PTI6_SOLLC (Pathogenesis-related genes transcriptional activator PTI6 OS=Solanum lycopersicum OX=4081 GN=PTI6 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 3.2e-32
Identity = 94/222 (42.34%), Postives = 122/222 (54.95%), Query Frame = 0


Query: 31  QKLVRIIVTDADATDSSSEDELILGSRTGVRRQVREITINRYSVLDGSSAESPXXXXXXX 90
           Q++VRII+TDADATDSS ++      R  VRR  R +T       + +   S        
Sbjct: 35  QRVVRIILTDADATDSSDDE-----GRNTVRRVKRHVT-------EINLMPSTKSIGDRK 94

Query: 91  XXXXXXXXXXXCRKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEAAAVYDRAAL 150
                       RKKFRGVRQR WGRWAAEIRDP R KR+WLGT+DT EEAA VYD+AA+
Sbjct: 95  RRSVSPDSDVTRRKKFRGVRQRPWGRWAAEIRDPTRGKRVWLGTYDTPEEAAVVYDKAAV 154

Query: 151 ELQGPNAITNF----SSDGAVTTAADGGNKQEDGLDSPKTTAVWSPASVLHYDGVFTPID 210
           +L+GP+A+TNF    +++              DG D  +     SP SVL  D  F P D
Sbjct: 155 KLKGPDAVTNFPVSTTAEXXXXXXXXXXXXXADGGDKSENDVALSPTSVL-CDNDFAPFD 214

Query: 211 EMLYCGEIDEFEFAM-ATASLP--AARRQYGGEEDFGELELD 246
            + +C E+D F F + +   LP  A   +Y G+E FGE + D
Sbjct: 215 NLGFC-EVDAFGFDVDSLFRLPDFAMTEKYYGDE-FGEFDFD 241

[Close]

BLAST of Carg03404-RA vs. Swiss-Prot
Match: sp|Q9SUE3|CRF4_ARATH (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 8.1e-28
Identity = 68/140 (48.57%), Postives = 89/140 (63.57%), Query Frame = 0


Query: 26  SAKLEQKLVRIIVTDADATDSSSEDELILGSRTGVRRQVREITINRYSVLDGSSAESPXX 85
           S +   +LVR+ +TD DATDSSS++E  L  R  V+R + EI +   S   G  + SP  
Sbjct: 35  SVRDSARLVRVSMTDRDATDSSSDEEEFLFPRRRVKRLINEIRVEPSSSSTGDVSASPTK 94

Query: 86  XXXXXXXXXXXXXXXXC---RKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEAA 145
                               +KK+RGVRQR WG+WAAEIRDP +R+RIWLGTF TAEEAA
Sbjct: 95  DRKRINVDSTVQKPSVSGQNQKKYRGVRQRPWGKWAAEIRDPEQRRRIWLGTFATAEEAA 154

Query: 146 AVYDRAALELQGPNAITNFS 163
            VYD AA++L+GP+A+TNF+
Sbjct: 155 IVYDNAAIKLRGPDALTNFT 174

[Close]

BLAST of Carg03404-RA vs. Swiss-Prot
Match: sp|Q9FK12|CRF3_ARATH (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 8.4e-25
Identity = 71/145 (48.97%), Postives = 88/145 (60.69%), Query Frame = 0


Query: 32  KLVRIIVTDADATDSSS--EDELILGSRTGVRRQVREITI--NRYSVLDG---------- 91
           K V I  TD DATDSSS  ++E  L  R  V+R V EIT+  +  +V+ G          
Sbjct: 37  KKVSICYTDPDATDSSSDEDEEDFLFPRRRVKRFVNEITVEPSCNNVVTGVSMKDRKRLS 96

Query: 92  SSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDT 151
           SS++                      KKFRGVRQR WG+WAAEIRDP +R+RIWLGTF+T
Sbjct: 97  SSSDETQSPASSRQRPNNKVSVSGQIKKFRGVRQRPWGKWAAEIRDPEQRRRIWLGTFET 156

Query: 152 AEEAAAVYDRAALELQGPNAITNFS 163
           AEEAA VYD AA+ L+GP+A+TNFS
Sbjct: 157 AEEAAVVYDNAAIRLRGPDALTNFS 181

[Close]

BLAST of Carg03404-RA vs. Swiss-Prot
Match: sp|Q9SUQ2|CRF2_ARATH (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.4e-21
Identity = 58/146 (39.73%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 30  EQKLVRIIVTDADATD-----SSSEDELILGSRTGVRRQVREITINRYSVLDGSSAESPX 89
           + +++RI VTD DATD                    ++ V E+ ++  +V+ GS  +   
Sbjct: 33  QTRILRISVTDPDATDXXXXXXXXXXXXXXXXXXXXKKFVNEVYLDSGAVVTGSCGQMES 92

Query: 90  XXXXXXXXXXXXXXXXXC--------RKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFD 149
                                      KKFRGVRQR WG+WAAEIRDP++R R+WLGT++
Sbjct: 93  KKRQKRAVKSESTVSPVVSAXXXXTGEKKFRGVRQRPWGKWAAEIRDPLKRVRLWLGTYN 152

Query: 150 TAEEAAAVYDRAALELQGPNAITNFS 163
           TAEEAA VYD AA++L+GP+A+TNFS
Sbjct: 153 TAEEAAMVYDNAAIQLRGPDALTNFS 178

[Close]

BLAST of Carg03404-RA vs. Swiss-Prot
Match: sp|O82503|CRF1_ARATH (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.7e-20
Identity = 45/58 (77.59%), Postives = 51/58 (87.93%), Query Frame = 0


Query: 104 KKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNF 162
           +KFRGVRQR WG+WAAEIRDP RR R+WLGTFDTAEEAA VYD AA++L+GPNA  NF
Sbjct: 85  RKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVYDNAAIQLRGPNAELNF 142

[Close]

BLAST of Carg03404-RA vs. TrEMBL
Match: tr|A0A1S4E155|A0A1S4E155_CUCME (ethylene-responsive transcription factor ERF069-like OS=Cucumis melo OX=3656 GN=LOC103496254 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.0e-92
Identity = 180/255 (70.59%), Postives = 196/255 (76.86%), Query Frame = 0


Query: 1   MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60
           MS  DMASLS  + NCRRK   SGDS K +Q+L+RIIVTDADATDSSSEDELILGSRT +
Sbjct: 1   MSVGDMASLSEHKTNCRRKALTSGDSTKPKQRLLRIIVTDADATDSSSEDELILGSRTTI 60

Query: 61  RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAE 120
           RRQVREITI R+SV D S                        R KFRGVRQR WGRWAAE
Sbjct: 61  RRQVREITIKRHSVADSSPKSPVSEICKKRNPRSRRSNNSSRRNKFRGVRQRPWGRWAAE 120

Query: 121 IRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDG 180
           +RDPI RKRIWLGTFDTAEEAAAVYDRAA+ELQGPNA TNFS DGAV TA DGG+K+EDG
Sbjct: 121 VRDPILRKRIWLGTFDTAEEAAAVYDRAAIELQGPNAATNFSGDGAVKTAVDGGSKKEDG 180

Query: 181 LDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQYGGEEDFG 240
           ++S KTTA WSP SVLHYD   TPI+EM+YCGE+DE    MA ASLPAARRQ  GEED G
Sbjct: 181 VESRKTTAAWSPTSVLHYDSFLTPIEEMVYCGEVDELGLEMAAASLPAARRQCCGEEDLG 240

Query: 241 ELELDLDYFLVDVIY 256
           E+ELDLDYFLVDVIY
Sbjct: 241 EMELDLDYFLVDVIY 255

[Close]

BLAST of Carg03404-RA vs. TrEMBL
Match: tr|A0A0A0KB59|A0A0A0KB59_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G133770 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.6e-84
Identity = 171/263 (65.02%), Postives = 187/263 (71.10%), Query Frame = 0


Query: 1   MSAADMASLSGDRANCRRKPAASGDSAKLEQKLVRIIVTDADATDSSSEDELILGSRTGV 60
           MS  DMASLSG + NCRRK   SG+S K  Q+L+RIIVTDADATDSSSEDELILGSRT +
Sbjct: 19  MSVGDMASLSGHKTNCRRKALTSGESTKPNQRLLRIIVTDADATDSSSEDELILGSRTAI 78

Query: 61  RRQVREITINRYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRK-KFRGVRQRAWGRWAA 120
           RRQVREITI RYSV D SS +SP                  CR+ KFRGVRQR WGRWAA
Sbjct: 79  RRQVREITIKRYSVPDSSSPKSPVSEICKKRNPRSRRSNNSCRRNKFRGVRQRPWGRWAA 138

Query: 121 EIRDPIRRKRIWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADG------ 180
           E+RDPI RKRIWLGTFDTAEEAAAVYDRAA+ELQGPNA TNFS DGAV +A +G      
Sbjct: 139 EVRDPILRKRIWLGTFDTAEEAAAVYDRAAIELQGPNAATNFSGDGAVKSAVEGXXXXXX 198

Query: 181 -GNKQEDGLDSPKTTAVWSPASVLHYDGVFTPIDEMLYCGEIDEFEFAMATASLPAARRQ 240
                            WSP SVLHYD   TPI+EM YCGE+DE    +  ASLP ARRQ
Sbjct: 199 XXXXXXXXXXXXXXXXAWSPTSVLHYDSFLTPIEEMGYCGEVDELGLEIGAASLPTARRQ 258

Query: 241 YGGEEDFGELELDLDYFLVDVIY 256
           YGGEE+ GE+ELDLDYFLVDVIY
Sbjct: 259 YGGEEELGEIELDLDYFLVDVIY 281

[Close]

BLAST of Carg03404-RA vs. TrEMBL
Match: tr|A0A200QTC6|A0A200QTC6_9MAGN (AP2/ERF domain OS=Macleaya cordata OX=56857 GN=BVC80_1767g13 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 3.8e-38
Identity = 104/228 (45.61%), Postives = 135/228 (59.21%), Query Frame = 0


Query: 28  KLEQKLVRIIVTDADATDSSSEDELILGSRTGVRRQVREITINRYSVLDGSSAESPXXXX 87
           K+ QK+VRII TD DATDSSS+DE     R  V+R +R+I      ++  ++ +      
Sbjct: 42  KIRQKVVRIIFTDVDATDSSSDDEEEFVRR--VKRHIRQI-----DIVSPAAKKRRKVSS 101

Query: 88  XXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEAAAVYDR 147
                          +K+FRGVRQR WGRWAAEIRDP R KR+WLGT+DT EEAAAVYD 
Sbjct: 102 VNSKRFRLSESDERHQKRFRGVRQRPWGRWAAEIRDPTRGKRLWLGTYDTPEEAAAVYDN 161

Query: 148 AALELQGPNAITNFSSDGAVTTAADGGNKQEDGLDSPKTTAVWSPASVLHYDGVFTPIDE 207
           AA+ L+GP+AITNF S     T         +G D  KTTA  SP SVL Y G FTP++ 
Sbjct: 162 AAVRLKGPDAITNFPSVSQTQTTV-----TSNGDDFSKTTAASSPTSVLRY-GDFTPLES 221

Query: 208 MLYCGEIDEFEFAM-ATASLPAARRQYGGEEDFGELELDLDYFLVDVI 255
             Y G++D F F    + +LP   ++Y  EEDF   E D+D F ++V+
Sbjct: 222 FGY-GDVDAFGFDFELSLTLPELPKKYFAEEDFS--EFDMDDFSLEVV 253

[Close]

BLAST of Carg03404-RA vs. TrEMBL
Match: tr|A0A2R6P6H8|A0A2R6P6H8_ACTCH (Pathogenesis-related transcriptional activator like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc31880 PE=4 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.7e-36
Identity = 109/238 (45.80%), Postives = 143/238 (60.08%), Query Frame = 0


Query: 23  SGDSA-KLEQKLVRIIVTDADATDSSSEDELILGSRTGVRRQVREITINRYSVLDGSSAE 82
           SGDS+    +++VRII+TDADATDSS +D+     R  V+R V+EI IN+      S  +
Sbjct: 3   SGDSSMAFRRRVVRIILTDADATDSSGDDDKQAVRR--VKRLVKEININQ------SPNK 62

Query: 83  SPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAEIRDPIRRKRIWLGTFDTAEEA 142
                                RKKFRGVR+R WGRWAAEIRDP RRKR+WLGT+DT EEA
Sbjct: 63  LEQSKKRKFRSLGSAGSDAVRRKKFRGVRRRPWGRWAAEIRDPTRRKRVWLGTYDTPEEA 122

Query: 143 AAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDGLDSPKTTAVWSPASVLHYDGV 202
           A VYDRAA++L+GP+A+TNF +   V+   D  + + DG  S    A+ SP SVL YD +
Sbjct: 123 ATVYDRAAVKLKGPDAVTNFPN---VSVTEDAVDVKTDGEGSANVAAL-SPTSVLCYDDL 182

Query: 203 FTPIDEMLYCGEIDEFEF------AMATASLPAARRQYGGEEDFGELELDLDYFLVDV 254
            TP D   Y G++D F F      ++A  ++P     + G +D GE E +LD FLVDV
Sbjct: 183 -TPFDGFSY-GDVDAFGFDIDKPLSLADFAVP---ENFYGADDLGE-EFNLDDFLVDV 222

[Close]

BLAST of Carg03404-RA vs. TrEMBL
Match: tr|A0A2R6QJI8|A0A2R6QJI8_ACTCH (Pathogenesis-related transcriptional activator like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc16586 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.5e-36
Identity = 112/247 (45.34%), Postives = 141/247 (57.09%), Query Frame = 0


Query: 23  SGDSA-KLEQKLVRIIVTDADATDSSSEDELILGSRTGVRRQVREITIN----------- 82
           SGDS+    +++VR+I TDADATDSS +DE     R  V+R V+EI IN           
Sbjct: 3   SGDSSTAFRRRVVRVIFTDADATDSSGDDEEQAVRR--VKRLVKEININPSPNKREQSKK 62

Query: 83  -RYSVLDGSSAESPXXXXXXXXXXXXXXXXXXCRKKFRGVRQRAWGRWAAEIRDPIRRKR 142
            R   L  + +ES                    RKKFRGVR+R WGRWAAEIRDP RRKR
Sbjct: 63  RRIGSLGSAGSESSR------------------RKKFRGVRRRPWGRWAAEIRDPTRRKR 122

Query: 143 IWLGTFDTAEEAAAVYDRAALELQGPNAITNFSSDGAVTTAADGGNKQEDGLDSPKTTAV 202
           +WLGT+DT EEAA VYDRAA++L+GP+A+TNF +   VT      + + DG  S    A+
Sbjct: 123 VWLGTYDTPEEAATVYDRAAVKLKGPDAVTNFPN---VTVTEKAVHVKTDGEGSDNVAAL 182

Query: 203 WSPASVLHYDGVFTPIDEMLYCGEIDEFEF----AMATASLPAARRQYGGEEDFGELELD 253
            SP SVL YD + TP DE  Y G++D F F     +  A        Y G++D GE E +
Sbjct: 183 -SPTSVLLYDEL-TPFDEFSY-GDVDAFGFDIDMPLRLADFTVPENLY-GDDDLGE-EFN 221

The following BLAST results are available for this feature:

BLAST of Carg03404-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022944036.1	2.1e-125	99.61	ethylene-responsive transcription factor ERF069-like [Cucurbita moschata]	[more]
XP_023512530.1	5.2e-124	91.76	ethylene-responsive transcription factor ERF069-like [Cucurbita pepo subsp. pepo...	[more]
XP_022986928.1	7.0e-121	89.80	ethylene-responsive transcription factor ERF069-like [Cucurbita maxima]	[more]
XP_016901953.1	3.1e-92	70.59	PREDICTED: ethylene-responsive transcription factor ERF069-like [Cucumis melo]	[more]
XP_022947817.1	1.1e-86	70.59	pathogenesis-related genes transcriptional activator PTI6-like [Cucurbita moscha...	[more]

BLAST of Carg03404-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT4G27950.1	4.5e-29	48.57	cytokinin response factor 4	[more]
AT5G53290.1	4.7e-26	48.97	cytokinin response factor 3	[more]
AT4G23750.1	4.1e-22	39.73	cytokinin response factor 2	[more]
AT4G11140.1	2.0e-21	77.59	cytokinin response factor 1	[more]
AT3G61630.1	5.0e-20	45.88	cytokinin response factor 6	[more]

BLAST of Carg03404-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O04682\|PTI6_SOLLC	3.2e-32	42.34	Pathogenesis-related genes transcriptional activator PTI6 OS=Solanum lycopersicu...	[more]
sp\|Q9SUE3\|CRF4_ARATH	8.1e-28	48.57	Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
sp\|Q9FK12\|CRF3_ARATH	8.4e-25	48.97	Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
sp\|Q9SUQ2\|CRF2_ARATH	7.4e-21	39.73	Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
sp\|O82503\|CRF1_ARATH	3.7e-20	77.59	Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Carg03404-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4E155\|A0A1S4E155_CUCME	2.0e-92	70.59	ethylene-responsive transcription factor ERF069-like OS=Cucumis melo OX=3656 GN=...	[more]
tr\|A0A0A0KB59\|A0A0A0KB59_CUCSA	1.6e-84	65.02	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G133770 PE=4 SV=1	[more]
tr\|A0A200QTC6\|A0A200QTC6_9MAGN	3.8e-38	45.61	AP2/ERF domain OS=Macleaya cordata OX=56857 GN=BVC80_1767g13 PE=4 SV=1	[more]
tr\|A0A2R6P6H8\|A0A2R6P6H8_ACTCH	2.7e-36	45.80	Pathogenesis-related transcriptional activator like OS=Actinidia chinensis var. ...	[more]
tr\|A0A2R6QJI8\|A0A2R6QJI8_ACTCH	3.5e-36	45.34	Pathogenesis-related transcriptional activator like OS=Actinidia chinensis var. ...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR016177	DNA-bd_dom_sf
IPR017392	AP2/ERF-transcript_factor
IPR036955	AP2/ERF_dom_sf
IPR001471	AP2/ERF_dom

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg03404	Carg03404	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg03404-RA.exon.1	Carg03404-RA.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg03404-RA.CDS.1	Carg03404-RA.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg03404-RA	Carg03404-RA-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 128..144 score: 65.99 coord: 106..117 score: 61.97
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 105..168 e-value: 5.3E-32 score: 122.3
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 105..154 e-value: 8.2E-13 score: 48.3
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 105..162 score: 23.367
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 106..161 e-value: 1.90831E-28 score: 103.12
IPR036955	AP2/ERF domain superfamily	GENE3D	G3DSA:3.30.730.10		coord: 104..163 e-value: 8.0E-31 score: 107.7
IPR017392	AP2/ERF transcription factor ERF/PTI6	PIRSF	PIRSF038123	PTI6	coord: 7..252 e-value: 2.4E-50 score: 169.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 90..104
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 80..104
None	No IPR available	PANTHER	PTHR31194	SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR	coord: 25..253
None	No IPR available	PANTHER	PTHR31194:SF18	SUBFAMILY NOT NAMED	coord: 25..253
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	SSF54171	DNA-binding domain	coord: 105..162