Carg10471-RA (mRNA) Silver-seed gourd

NameCarg10471-RA
TypemRNA
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionGdsl esterase/lipase
LocationCucurbita_argyrosperma_scaffold_098 : 364329 .. 368992 (-)
Sequence length2130
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTGTTCTCCGCCTTCTCTTCTCTCTCCTCCTCCTCATCTCTCTCTTCTCAGGTTTGGATTCTGTGCTTATTTGAATTACTTCACAATTTCTGTTTGTTCTCTCTCTAATTTCTGCCATTTAGGACGAGTATCGGCTGGTGGCGTTTATAATCACCACCACCACCACCACCAGCGCCACCGTCTCCGGCCAACAAAGCTGTTTGTTTTCGGGGATTCCTATGTTGATACTGGCAACATTCTTATTCCCCTTTCCAGCGCTCAGCAATTTCCTTATGGAATCACTTTTCCAGGAAAACCCAGTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGGTAAACAAATCTCTGTCTCTGTTTTTGTCTCTCTCTGTCTCACCGATTTTCCGACCGTCCTGTTCTTACCAAACACAAATGGCTGTCACACAAGGCCTTAAAATGATCTTAATCTTGAAATGTAATGGAACGTATGGGAATAATTGAATGCAGCGAGATATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACATCGTTTCCTTTCCCAAACATGACAACCCAAATCGACTTCTTTCAACAGCTCCAACATGGCGAATCTCTGTCCACCAAAACAGACAACCAATTCTCCGCCGCACTCGTCTCTGTTTCCGGCAATGACTATTCCTTGGACATAATCACTAACGGCCCTAAACAGGTACCAATCAATTCCGTAATCTAATCCCAAGAGAATCATAAACTGAAATCCCGATGTGTTTTGCAGGGATTGAAACCGTTCATAAACACGGTAGTGAACCAGATCATCATGGATTTGAAACGCATTGGGGAGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGGTGTCTTCCGGCATTTACAGCTCCCTCGTCGTTCAAGAAATGCAATGAAACCATAAACTCGTTCGTGAAGTTTCACAACTTCTTGCTGAAGAAAGCAGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTGTTCTTGATATGTATGGGGCGTTCATGTCCATAATTGAAGGGGAGAAGAGATACAACAATGGAGTGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGTAAAGATCCCAAGAAAGCTTTCTATTGGGATGCAGTTCATCCTACACAACAAGGATGGGCTGCTGCGTTTTCCGCTTTAAAAGCCTTTCGCAACCATTTTTAAGTTGAATTAGTGGAGAATGATGTTATGGCATTGTGCCACACCCACCATAACAACATAACAACATGTACTAATAAACCTCTTCCATATCAAAATCAATTCACACATCTTTTCATCTAATTTTTTCGTGCAATATTAATGTTCGTTAAAATAAAATATGGGACAAGAACATACAAACCTCTAGACACGTTTTAAAATTATGAAAGAATATTCGAAAAAGCATTTAGGGAATCGGTTTGATTCTATCTTTGTCCCTTTGGTTTGAAGAAAGGAAGGATCCTAAAGAATTGATCTTTCTTTTAGTTGTTGAATCTCTCTTTGTTGATCCACCAGTGATATTCCAAATCTTGGTTACTAATAAAATCCAATTGAATAGAATCTATTACTTGAATGAAATTAGATCTCGTGGAATCATGTTGAATATTTGACAAGATATTCTGTGCCTTGCTAAAAAAAACGTTCCTTGTTTACCAACCACACATTATCTAATGAAATCTAATTGGGGCTAATGACGATACATATGGGACCAAAATGGACAAATATTTACTCGGGGTAGAATTTGATTGTTACAAATGGTATTAGAGCTGGGTAAGAGGGGCGGATTGTGAGAGGGAAACATAAATGAAGAATTTCATGGAGATGAGATGAAATGAGAGGCGATGTAATCTTCTTTCATCTTTAACTTACCGGGTGAATATCCCTAGTTGGAATAATTTTCTTATTTACTTCTTTGCAATAATTTTTTAAAAGGTTAAATTAATAAAAAAACACATATAAATTTTTATTTTATCGGACTTCCAAATTTTCAATAATAACCTTAAACTAAAAAAAATCTTAAAAAATAAATTTGGAAAATGTTTCTTTTAAAAATTTTAAAATACAAACTTTAAAAAAAAAAATGAGAGAGGAAGTCAGAGGTGAGAGAAAACACACGCTCAAGATGAGAGAGAAGAAGAAAAAAAAAGGGTGCAAAGCCCACGTGGCCCAACATGGGCCGCACATCCCATGCGGCCCAGCTCGGGCACTCGGCCCAACGGGCGTGTGGCCGACCCGACGACCCGCCCGGTAGTGGGCCGAACCGATAGCCCAACCCGGTTATGTCCAATTGGGCCAGTCTGACCTGGTTCTGTTCAATTGGACCAATTTTGTTCCTTTTGCGTAATTTTCTCGTTTCAACTCCGATTTGGAATTATTTGAGGATTTTAGAGTTATTAAGAATGCAAATCCGAACAAAAAGTGTCATACTTTTTAATTAGGTGGCACCTTAAAACCTTAGATTTAACTATGCTCGCAGTATGTATGAAAGTATGATATGAGAGTATGTCATAGAAATTTGAGTATGAAATGAGTTGGTTTAATGTATGTAAGAGTACGTTAAATGTTATGATGTACATTTATCAAGAAACTCGATTATATATGTTTGAGCATAGAATGAGTTGAGACAACACATATTGAATGTGATTATGTATGTTTACTGAAATGGCACTATTTTATGACTAGATATTCATATTTAGATTGCTATTATGTCTCTATATCATAAGTTTACGATGTATACAGTTATATGGGGATGATTAGGAGTTCGACAACTAACCTTTAATCGTGAACCTGACTCGATTGACAGGAGTTTGAATAACCATACCGTCATTATTGAACGGGTGGAAATTTGAAAACAGAAAAACAAACTAGAAACAAAAATTACCAAACAATTCATTTAAGGATGAAAAACAACTGTCCAAAGTGATGTCGTTTGTTTGTCGTTAAACATTACATGTATATATTGTCGAGTCTTCACCGATTGTATTCAAGCTTACAAGAACAAGCGGCTTCTCCGCCGGCCACCGCTTAACGTTGTGAAAGCATTCGATCCAGTAATCAAACACCTGCAACAATTTCCCTTTCACCTTATAATTCAATCATTATCAATATGATGGATTTTGTTCTCCGCCTTCTCTTCTCTCTCCTCCTTCTCATCTCTCTTTTCTCAGGTTTGGATTCTGTGCTTATTTCATTTACTTCACAATTTCTGTTTCTTCTCTCTCTAATTTCTTCCATTTAGGAAGAGGATCGGCTGGTGGCGTTTACAATCACCACCACCACCGCCGCCGCCATCTCCGCCACCGTTTCCGGCCAACAAAGCTGTTTGTTTTTGGGGATTCCTATGCTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGCTCTGCAATTTCCTTATGGAATTACTTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGGTAAACAAATCTCTGTCTCTGTCTCTGTTTCTGTCTCTCTCTGTTTCGCCGATTTTCCGACCGTCCTGTTCTTACTAAAAAAAAATGGCTGTCAAACGAGGCCTTAAAATCATCTTAATCTCGAAATTTAATGGAACGTATGGGAATAATTGAATGCAGCGAGACATGTCGGGTTGAAATCTCCGATCCCATACAGTCTCCGAAACAGAGTTGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTCTTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCCCAATCCTCTGTCGCACTCGTCTCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGTATCAATCAATTCCGCAATCTAACCCCAAGAGAATCACAAACTGAAATTCCGATGTGTTTTACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACCATAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTTTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGTGTTCAAGACGCCCTTGAAGCCGTGTTGTCATGGAGTGGGGAGTGGATTTAGTTGCGGGAGTGTGGACGGAGAAGGGAAGAAGATGTTTGTGTTCTGTGAGGATCAGAAAGCAGCTTTCTTTTGGGATTCGGTGCATCCTACGCAACAAGGATGGGCCGCTGCTTTCTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

mRNA sequence

ATGGATTCTGTTCTCCGCCTTCTCTTCTCTCTCCTCCTCCTCATCTCTCTCTTCTCAGGACGAGTATCGGCTGGTGGCGTTTATAATCACCACCACCACCACCACCAGCGCCACCGTCTCCGGCCAACAAAGCTGTTTGTTTTCGGGGATTCCTATGTTGATACTGGCAACATTCTTATTCCCCTTTCCAGCGCTCAGCAATTTCCTTATGGAATCACTTTTCCAGGAAAACCCAGTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGCGAGATATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACATCGTTTCCTTTCCCAAACATGACAACCCAAATCGACTTCTTTCAACAGCTCCAACATGGCGAATCTCTGTCCACCAAAACAGACAACCAATTCTCCGCCGCACTCGTCTCTGTTTCCGGCAATGACTATTCCTTGGACATAATCACTAACGGCCCTAAACAGGGATTGAAACCGTTCATAAACACGGTAGTGAACCAGATCATCATGGATTTGAAACGCATTGGGGAGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGGTGTCTTCCGGCATTTACAGCTCCCTCGTCGTTCAAGAAATGCAATGAAACCATAAACTCGTTCGTGAAGTTTCACAACTTCTTGCTGAAGAAAGCAGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTGTTCTTGATATGTATGGGGCGTTCATGTCCATAATTGAAGGGGAGAAGAGATACAACAATGGAGTGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGTAAAGATCCCAAGAAAGCTTTCTATTGGGATGCAGTTCATCCTACACAACAAGGATGGGCTGCTGCGTTTGGATTCTGTGCTTATTTCATTTACTTCACAATTTCTGTTTCTTCTCTCTCTAATTTCTTCCATTTAGGAAGAGGATCGGCTGGTGGCGTTTACAATCACCACCACCACCGCCGCCGCCATCTCCGCCACCGTTTCCGGCCAACAAAGCTGTTTGTTTTTGGGGATTCCTATGCTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGCTCTGCAATTTCCTTATGGAATTACTTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGCGAGACATGTCGGGTTGAAATCTCCGATCCCATACAGTCTCCGAAACAGAGTTGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTCTTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCCCAATCCTCTGTCGCACTCGTCTCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACCATAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTTTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGTGTTCAAGACGCCCTTGAAGCCGTGTTGTCATGGAGTGGGGAGTGGATTTAGTTGCGGGAGTGTGGACGGAGAAGGGAAGAAGATGTTTGTGTTCTGTGAGGATCAGAAAGCAGCTTTCTTTTGGGATTCGGTGCATCCTACGCAACAAGGATGGGCCGCTGCTTTCTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

Coding sequence (CDS)

ATGGATTCTGTTCTCCGCCTTCTCTTCTCTCTCCTCCTCCTCATCTCTCTCTTCTCAGGACGAGTATCGGCTGGTGGCGTTTATAATCACCACCACCACCACCACCAGCGCCACCGTCTCCGGCCAACAAAGCTGTTTGTTTTCGGGGATTCCTATGTTGATACTGGCAACATTCTTATTCCCCTTTCCAGCGCTCAGCAATTTCCTTATGGAATCACTTTTCCAGGAAAACCCAGTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGCGAGATATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACATCGTTTCCTTTCCCAAACATGACAACCCAAATCGACTTCTTTCAACAGCTCCAACATGGCGAATCTCTGTCCACCAAAACAGACAACCAATTCTCCGCCGCACTCGTCTCTGTTTCCGGCAATGACTATTCCTTGGACATAATCACTAACGGCCCTAAACAGGGATTGAAACCGTTCATAAACACGGTAGTGAACCAGATCATCATGGATTTGAAACGCATTGGGGAGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGGTGTCTTCCGGCATTTACAGCTCCCTCGTCGTTCAAGAAATGCAATGAAACCATAAACTCGTTCGTGAAGTTTCACAACTTCTTGCTGAAGAAAGCAGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTGTTCTTGATATGTATGGGGCGTTCATGTCCATAATTGAAGGGGAGAAGAGATACAACAATGGAGTGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGTAAAGATCCCAAGAAAGCTTTCTATTGGGATGCAGTTCATCCTACACAACAAGGATGGGCTGCTGCGTTTGGATTCTGTGCTTATTTCATTTACTTCACAATTTCTGTTTCTTCTCTCTCTAATTTCTTCCATTTAGGAAGAGGATCGGCTGGTGGCGTTTACAATCACCACCACCACCGCCGCCGCCATCTCCGCCACCGTTTCCGGCCAACAAAGCTGTTTGTTTTTGGGGATTCCTATGCTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGCTCTGCAATTTCCTTATGGAATTACTTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGCGAGACATGTCGGGTTGAAATCTCCGATCCCATACAGTCTCCGAAACAGAGTTGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTCTTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCCCAATCCTCTGTCGCACTCGTCTCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACCATAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTTTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGTGTTCAAGACGCCCTTGAAGCCGTGTTGTCATGGAGTGGGGAGTGGATTTAGTTGCGGGAGTGTGGACGGAGAAGGGAAGAAGATGTTTGTGTTCTGTGAGGATCAGAAAGCAGCTTTCTTTTGGGATTCGGTGCATCCTACGCAACAAGGATGGGCCGCTGCTTTCTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

Protein sequence

MDSVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHHQRHRLRPTKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFGFCAYFIYFTISVSSLSNFFHLGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF
BLAST of Carg10471-RA vs. NCBI nr
Match: XP_023543956.1 (GDSL esterase/lipase At5g03610-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678.7 bits (1750), Expect = 2.1e-191
Identity = 341/363 (93.94%), Postives = 347/363 (95.59%), Query Frame = 0

Query: 347 FGFCAYFIYFTISVSSLSNFFHLGRGSAGXXXXXXXXXXXXLRHRFRPTKLFVFGDSYAD 406
           FGFCAYFIYFTISV SLSNFFHLGRGSAG   XXXXXXXXX    FRPTKLFVFGDSYAD
Sbjct: 22  FGFCAYFIYFTISVCSLSNFFHLGRGSAG---XXXXXXXXXXXXXFRPTKLFVFGDSYAD 81

Query: 407 TGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPYSLRNRVGLRR 466
           TGNILFPISP LQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIP+S+RNRVG RR
Sbjct: 82  TGNILFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGSRR 141

Query: 467 FKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGND 526
           FKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGND
Sbjct: 142 FKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGND 201

Query: 527 YSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSY 586
           YSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSY
Sbjct: 202 YSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSY 261

Query: 587 KKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGVFK 646
           KKCNET+NSLVQFHNFLLKE VKKLNQETKHSSKVFIVDVYGAFMSII+GRKRYNNG FK
Sbjct: 262 KKCNETVNSLVQFHNFLLKEGVKKLNQETKHSSKVFIVDVYGAFMSIIQGRKRYNNGEFK 321

Query: 647 TPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKSFR 706
           TPLKPCC GV SGF CGSVDGEGKKMFV C+D+KAAFFWDSVHPTQQGWAAAFSAFKSFR
Sbjct: 322 TPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFR 381

Query: 707 NHF 710
           NHF
Sbjct: 382 NHF 381

BLAST of Carg10471-RA vs. NCBI nr
Match: XP_022925652.1 (GDSL esterase/lipase At5g03610-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 662.5 bits (1708), Expect = 1.6e-186
Identity = 341/347 (98.27%), Postives = 344/347 (99.14%), Query Frame = 0

Query: 1   MDSVLRLLFSLLLLISLFSGRVSAXXXXXXXXXXXXXXRLRPTKLFVFGDSYVDTGNILI 60
           MD VLRLLFSLLLLISLFSGRVSA  XXXXXXXXXXXXRLRPTKLFVFGDSYVDTGNILI
Sbjct: 1   MDFVLRLLFSLLLLISLFSGRVSA-GXXXXXXXXXXXXRLRPTKLFVFGDSYVDTGNILI 60

Query: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFSVRNRVGLRRFKETGV 120
           PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPF+V+NRVGLRRFKETGV
Sbjct: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGV 120

Query: 121 NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII 180
           NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII
Sbjct: 121 NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII 180

Query: 181 TNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET 240
           TNGPKQGLKPFINTVVNQII+DLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET
Sbjct: 181 TNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET 240

Query: 241 INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC 300
           INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC
Sbjct: 241 INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC 300

Query: 301 CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF 348
           CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF
Sbjct: 301 CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF 346

BLAST of Carg10471-RA vs. NCBI nr
Match: XP_022925650.1 (GDSL esterase/lipase At5g03610-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 647.9 bits (1670), Expect = 4.0e-182
Identity = 310/322 (96.27%), Postives = 318/322 (98.76%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH
Sbjct: 39  LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 98

Query: 448 VGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSV 507
           VGLKSPIP+S+RNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDS+
Sbjct: 99  VGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSI 158

Query: 508 FTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA 567
           FTNTDAQSSVALVSASGNDY+FYFAMNSSRQGLKPFIMSVV+QIIVNLKRIGGLGVKKIA
Sbjct: 159 FTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIA 218

Query: 568 VTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVY 627
           VTGLGPLGCLPVFTAPSSYK+CNETINSLVQFHN LLKEAVKKLNQETKHSSKVF+VDVY
Sbjct: 219 VTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVY 278

Query: 628 GAFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 687
           GAFMSIIEGRKRYNNG FKTPLKPCC+GVGSGFSCGSVDGEGKKMFV CEDQKAAFFWDS
Sbjct: 279 GAFMSIIEGRKRYNNGEFKTPLKPCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDS 338

Query: 688 VHPTQQGWAAAFSAFKSFRNHF 710
           VHPTQQGWAAA SAFKSFRNHF
Sbjct: 339 VHPTQQGWAAAISAFKSFRNHF 360

BLAST of Carg10471-RA vs. NCBI nr
Match: XP_022925651.1 (GDSL esterase/lipase At5g03610-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 647.9 bits (1670), Expect = 4.0e-182
Identity = 310/322 (96.27%), Postives = 318/322 (98.76%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH
Sbjct: 38  LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 97

Query: 448 VGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSV 507
           VGLKSPIP+S+RNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDS+
Sbjct: 98  VGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSI 157

Query: 508 FTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA 567
           FTNTDAQSSVALVSASGNDY+FYFAMNSSRQGLKPFIMSVV+QIIVNLKRIGGLGVKKIA
Sbjct: 158 FTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIA 217

Query: 568 VTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVY 627
           VTGLGPLGCLPVFTAPSSYK+CNETINSLVQFHN LLKEAVKKLNQETKHSSKVF+VDVY
Sbjct: 218 VTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVY 277

Query: 628 GAFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 687
           GAFMSIIEGRKRYNNG FKTPLKPCC+GVGSGFSCGSVDGEGKKMFV CEDQKAAFFWDS
Sbjct: 278 GAFMSIIEGRKRYNNGEFKTPLKPCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDS 337

Query: 688 VHPTQQGWAAAFSAFKSFRNHF 710
           VHPTQQGWAAA SAFKSFRNHF
Sbjct: 338 VHPTQQGWAAAISAFKSFRNHF 359

BLAST of Carg10471-RA vs. NCBI nr
Match: XP_023544846.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 643.7 bits (1659), Expect = 7.6e-181
Identity = 307/322 (95.34%), Postives = 314/322 (97.52%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH
Sbjct: 38  LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 97

Query: 448 VGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSV 507
           VGLKSPIP+S+RNRVGLRR KETGVNFAFGGTGVFDTEYP PNMTTQIDFFQQLRHGDS+
Sbjct: 98  VGLKSPIPFSVRNRVGLRRLKETGVNFAFGGTGVFDTEYPFPNMTTQIDFFQQLRHGDSI 157

Query: 508 FTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA 567
           FTN DAQSSVALVSASGNDYSFYFAMNS+RQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA
Sbjct: 158 FTNKDAQSSVALVSASGNDYSFYFAMNSTRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA 217

Query: 568 VTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVY 627
           VTGLGPLGCLPVFTAPSSYK+CNET+NSLVQFHN LLKE VKKLNQETKHSSKVFIVDVY
Sbjct: 218 VTGLGPLGCLPVFTAPSSYKQCNETVNSLVQFHNLLLKEGVKKLNQETKHSSKVFIVDVY 277

Query: 628 GAFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 687
           GAFMSIIEGRKRYNNG FKTPLKPCCHGVGSGFSCGSVDGEGKKM+V CED KAAFFWDS
Sbjct: 278 GAFMSIIEGRKRYNNGEFKTPLKPCCHGVGSGFSCGSVDGEGKKMYVLCEDHKAAFFWDS 337

Query: 688 VHPTQQGWAAAFSAFKSFRNHF 710
           VHPTQQGWAAAFSAFKSFRNHF
Sbjct: 338 VHPTQQGWAAAFSAFKSFRNHF 359

BLAST of Carg10471-RA vs. TAIR10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 353.2 bits (905), Expect = 3.7e-97
Identity = 174/314 (55.41%), Postives = 225/314 (71.66%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           FRPTKLFVFGDSYADTGNI    S + +FPYGITFPGKP GRFSDGRV TDF A+ VG+K
Sbjct: 39  FRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIK 98

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNT 511
           SPIPY  ++  G +R  + G+NFA+GGTGVF+T+ PLPNMTTQID FQ +     ++   
Sbjct: 99  SPIPYFWKDYAGKKRL-QYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPP 158

Query: 512 DAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGL 571
           +  SSVALVS +GNDYS + A+N        FI  VV+Q  VNL+RI  LGVKKIAV  L
Sbjct: 159 ELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSL 218

Query: 572 GPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFM 631
            PLGCLP FT  +S+++CNET N+LV  HN LL++ V KLN ETK S+ + I+D+Y AF+
Sbjct: 219 QPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQSTFI-ILDLYNAFL 278

Query: 632 SIIEGRKRYNNGV--FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVH 691
           ++ +  K  N G   F++PLKPCC GV   ++CGSVD +G K ++ C++ K AFFWD +H
Sbjct: 279 TVFK-NKGSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLH 338

Query: 692 PTQQGWAAAFSAFK 704
           PT++GW + +S  +
Sbjct: 339 PTEEGWRSVYSVLR 349

BLAST of Carg10471-RA vs. TAIR10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 323.6 bits (828), Expect = 3.1e-88
Identity = 159/313 (50.80%), Postives = 217/313 (69.33%), Query Frame = 0

Query: 391 RFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGL 450
           R RP +LFVFGDSYADTGNI   +S + + PYGITFP KP GRFSDGRV TDF AR++G+
Sbjct: 34  RLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGI 93

Query: 451 KSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDT-EYPLPNMTTQIDFFQQLRHGDSVFT 510
           KSPIPY+ ++  G  R    G+N+A+GGTGVF T + PLPNMTTQID+FQ++    ++++
Sbjct: 94  KSPIPYTWKDYAGKERLL-YGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYS 153

Query: 511 NTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVT 570
            +D  SS+ALVS +GNDY+ + A+      L  F+  VV+QI VN  RI  LGV KI + 
Sbjct: 154 PSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIP 213

Query: 571 GLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGA 630
            + PLGCLP  T  +S+++CN T N+    HN+LL +A+ +LN ETK S+ V ++D Y A
Sbjct: 214 SMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNETKPSTFV-VLDHYNA 273

Query: 631 FMSIIEGRKRYNNGV--FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 690
           F+++ +  K    GV  F  PLKPCC GV S + C +VD +G+K ++ CED KAAFFWD 
Sbjct: 274 FLTVFK-NKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDI 333

Query: 691 VHPTQQGWAAAFS 701
            HP+++GW + +S
Sbjct: 334 FHPSEEGWRSVYS 343

BLAST of Carg10471-RA vs. TAIR10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 260.4 bits (664), Expect = 3.3e-69
Identity = 145/321 (45.17%), Postives = 199/321 (61.99%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LR R  P KLFVFGDSYADTGN     + A   PYGITFPGKP GR+ DG + TDF  + 
Sbjct: 7   LRPRKVP-KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKV 66

Query: 448 VGLKSPIPYSLRNR-VGLRRFKETGVNFAFGGTGVFDT--EYPLPNMTTQIDFFQQLRHG 507
           +G +SP  Y    R  GL+R    G+NFAFGG+ + D+    P PN+T Q++F   L   
Sbjct: 67  LGAESPYLYRTHGRDKGLKR----GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLA 126

Query: 508 DSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVK 567
             V+ +    S V+L+S +G DY +Y   N    GLK  +  VV+ + VN+  +GGL  K
Sbjct: 127 GRVYGDI-TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFK 186

Query: 568 KIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQET---KHSSKV 627
           KIAVT L P+GCLP +T+ SS+K CNE+ ++LV+ HN LLK+ V KLN+++   K     
Sbjct: 187 KIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF 246

Query: 628 FIVDVYGAFMSII--EGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQ 687
           FI+D++ AFM+++  +G KR     FK P+K CC G      CG    +G K++  C+D 
Sbjct: 247 FIIDIHNAFMTVMKNKGSKR-----FKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDP 306

Query: 688 KAAFFWDSVHPTQQGWAAAFS 701
           K+ FFWD+VHPTQ+GW + +S
Sbjct: 307 KSFFFWDAVHPTQEGWRSIYS 309

BLAST of Carg10471-RA vs. TAIR10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 249.2 bits (635), Expect = 7.5e-66
Identity = 137/312 (43.91%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           F+PTKLFVFGDSYADTGN  F I P+ +FP GITFPG P GRFSDGRV TD+ A+++G++
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPL-PNMTTQIDFFQQLRHGDSVFTN 511
           +PI Y        R   + G+NFA+GG G F+T + L P  + QID F+QL    +V++ 
Sbjct: 103 TPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSP 162

Query: 512 TDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTG 571
            D  SSVA  S  GNDY  Y   N S +G       VV QI++++KRI  LGV+K+ V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 572 LGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLN-QETKHSSKVFI-VDVYG 631
             P  CLP    P   K C +T ++    HN LL++ + KLN +E  ++ K F+ +D+Y 
Sbjct: 223 SPPQKCLPKLVTP---KGC-DTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 282

Query: 632 AFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSV 691
           AF++I + +       F  P K CC     G  CG     GKK++  C+D K+ FFWD+V
Sbjct: 283 AFVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWDNV 342

Query: 692 HPTQQGWAAAFS 701
           H + QGW + FS
Sbjct: 343 HISDQGWRSVFS 348

BLAST of Carg10471-RA vs. TAIR10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 145.2 bits (365), Expect = 1.5e-34
Identity = 97/292 (33.22%), Postives = 132/292 (45.21%), Query Frame = 0

Query: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFSVRNRVGLRRFKETGV 120
           P + + + PYGITFPGKPSGR+SDG   TDF                             
Sbjct: 3   PTALSWKLPYGITFPGKPSGRYSDGLTATDFL---------------------------- 62

Query: 121 NFAFGGTGVFDTSFP-FPNMTTQIDFFQQLQHGESLST-KTDNQFSAALVSVSGNDYSLD 180
                G+ VFD+     PN++TQ+ F   L     + T   D   S AL+S SG DY   
Sbjct: 63  -----GSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGF 122

Query: 181 IITNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCN 240
           I  N        F+  +V  I                                     CN
Sbjct: 123 IDQNPNMAAYPAFVEFIVEDI------------------------------------HCN 182

Query: 241 ETINSFVKFHNFLLKKAVKKLNQETKHSSK---VFVLDMYGAFMSIIEGEKRYNNGVFKT 300
           E+ +  V+ HN  LKKAV KLN+E K  +K     ++D++ AFM+I+E   +  N  FK+
Sbjct: 183 ESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILE---KKGNKRFKS 217

Query: 301 PLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF 348
           PLKPCC G     DC  +D +G K + LC DPK AF+WD ++PTQ+GW + +
Sbjct: 243 PLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIY 217

BLAST of Carg10471-RA vs. Swiss-Prot
Match: sp|Q9LZS7|GDL71_ARATH (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.7e-96
Identity = 174/314 (55.41%), Postives = 225/314 (71.66%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           FRPTKLFVFGDSYADTGNI    S + +FPYGITFPGKP GRFSDGRV TDF A+ VG+K
Sbjct: 39  FRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIK 98

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNT 511
           SPIPY  ++  G +R  + G+NFA+GGTGVF+T+ PLPNMTTQID FQ +     ++   
Sbjct: 99  SPIPYFWKDYAGKKRL-QYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPP 158

Query: 512 DAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGL 571
           +  SSVALVS +GNDYS + A+N        FI  VV+Q  VNL+RI  LGVKKIAV  L
Sbjct: 159 ELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSL 218

Query: 572 GPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFM 631
            PLGCLP FT  +S+++CNET N+LV  HN LL++ V KLN ETK S+ + I+D+Y AF+
Sbjct: 219 QPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQSTFI-ILDLYNAFL 278

Query: 632 SIIEGRKRYNNGV--FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVH 691
           ++ +  K  N G   F++PLKPCC GV   ++CGSVD +G K ++ C++ K AFFWD +H
Sbjct: 279 TVFK-NKGSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLH 338

Query: 692 PTQQGWAAAFSAFK 704
           PT++GW + +S  +
Sbjct: 339 PTEEGWRSVYSVLR 349

BLAST of Carg10471-RA vs. Swiss-Prot
Match: sp|Q9SF94|GDL50_ARATH (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.7e-87
Identity = 159/313 (50.80%), Postives = 217/313 (69.33%), Query Frame = 0

Query: 391 RFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGL 450
           R RP +LFVFGDSYADTGNI   +S + + PYGITFP KP GRFSDGRV TDF AR++G+
Sbjct: 34  RLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGI 93

Query: 451 KSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDT-EYPLPNMTTQIDFFQQLRHGDSVFT 510
           KSPIPY+ ++  G  R    G+N+A+GGTGVF T + PLPNMTTQID+FQ++    ++++
Sbjct: 94  KSPIPYTWKDYAGKERLL-YGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYS 153

Query: 511 NTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVT 570
            +D  SS+ALVS +GNDY+ + A+      L  F+  VV+QI VN  RI  LGV KI + 
Sbjct: 154 PSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIP 213

Query: 571 GLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGA 630
            + PLGCLP  T  +S+++CN T N+    HN+LL +A+ +LN ETK S+ V ++D Y A
Sbjct: 214 SMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNETKPSTFV-VLDHYNA 273

Query: 631 FMSIIEGRKRYNNGV--FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 690
           F+++ +  K    GV  F  PLKPCC GV S + C +VD +G+K ++ CED KAAFFWD 
Sbjct: 274 FLTVFK-NKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDI 333

Query: 691 VHPTQQGWAAAFS 701
            HP+++GW + +S
Sbjct: 334 FHPSEEGWRSVYS 343

BLAST of Carg10471-RA vs. Swiss-Prot
Match: sp|Q9LZS8|GDL70_ARATH (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 5.9e-68
Identity = 145/321 (45.17%), Postives = 199/321 (61.99%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LR R  P KLFVFGDSYADTGN     + A   PYGITFPGKP GR+ DG + TDF  + 
Sbjct: 7   LRPRKVP-KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKV 66

Query: 448 VGLKSPIPYSLRNR-VGLRRFKETGVNFAFGGTGVFDT--EYPLPNMTTQIDFFQQLRHG 507
           +G +SP  Y    R  GL+R    G+NFAFGG+ + D+    P PN+T Q++F   L   
Sbjct: 67  LGAESPYLYRTHGRDKGLKR----GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLA 126

Query: 508 DSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVK 567
             V+ +    S V+L+S +G DY +Y   N    GLK  +  VV+ + VN+  +GGL  K
Sbjct: 127 GRVYGDI-TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFK 186

Query: 568 KIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQET---KHSSKV 627
           KIAVT L P+GCLP +T+ SS+K CNE+ ++LV+ HN LLK+ V KLN+++   K     
Sbjct: 187 KIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF 246

Query: 628 FIVDVYGAFMSII--EGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQ 687
           FI+D++ AFM+++  +G KR     FK P+K CC G      CG    +G K++  C+D 
Sbjct: 247 FIIDIHNAFMTVMKNKGSKR-----FKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDP 306

Query: 688 KAAFFWDSVHPTQQGWAAAFS 701
           K+ FFWD+VHPTQ+GW + +S
Sbjct: 307 KSFFFWDAVHPTQEGWRSIYS 309

BLAST of Carg10471-RA vs. Swiss-Prot
Match: sp|Q9LZS9|GDL69_ARATH (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 2.5e-66
Identity = 143/311 (45.98%), Postives = 192/311 (61.74%), Query Frame = 0

Query: 396 KLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIP 455
           KLFVFG+SYADTGN + P + + + PYGITFPGKP GR+SDG   TDF A+ +G K  +P
Sbjct: 34  KLFVFGNSYADTGN-MKPTALSWKLPYGITFPGKPSGRYSDGLTATDFLAKQLGAK--LP 93

Query: 456 YSLRNRVGLRRFK-ETGVNFAFGGTGVFDTEYP-LPNMTTQIDFFQQLRHGDSVFT-NTD 515
           Y  R   G ++ K   G+NFAFGG+ VFD+     PN++TQ+ F   L     V+T + D
Sbjct: 94  YLWRTH-GKKKVKLNRGMNFAFGGSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGD 153

Query: 516 AQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLG 575
             SS AL+S SG DY  +   N +      F+  +V  I  +L  + GL  K IAVT L 
Sbjct: 154 LASSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLH 213

Query: 576 PLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSK---VFIVDVYGA 635
           PLGCLP  T  SS++ CNE+ + LV+ HN  LK+AV KLN+E K  +K     IVD++ A
Sbjct: 214 PLGCLPRVTVASSFRSCNESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKA 273

Query: 636 FMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVH 695
           FM+I+E   +  N  FK+PLKPCC G      C  +D +G K +  C D K+AFFWD ++
Sbjct: 274 FMTILE---KKGNKRFKSPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEIN 332

Query: 696 PTQQGWAAAFS 701
           PTQ+GW + +S
Sbjct: 334 PTQEGWRSIYS 332

BLAST of Carg10471-RA vs. Swiss-Prot
Match: sp|Q8RWJ4|GDL45_ARATH (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 249.2 bits (635), Expect = 1.4e-64
Identity = 137/312 (43.91%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           F+PTKLFVFGDSYADTGN  F I P+ +FP GITFPG P GRFSDGRV TD+ A+++G++
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPL-PNMTTQIDFFQQLRHGDSVFTN 511
           +PI Y        R   + G+NFA+GG G F+T + L P  + QID F+QL    +V++ 
Sbjct: 103 TPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSP 162

Query: 512 TDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTG 571
            D  SSVA  S  GNDY  Y   N S +G       VV QI++++KRI  LGV+K+ V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 572 LGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLN-QETKHSSKVFI-VDVYG 631
             P  CLP    P   K C +T ++    HN LL++ + KLN +E  ++ K F+ +D+Y 
Sbjct: 223 SPPQKCLPKLVTP---KGC-DTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 282

Query: 632 AFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSV 691
           AF++I + +       F  P K CC     G  CG     GKK++  C+D K+ FFWD+V
Sbjct: 283 AFVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWDNV 342

Query: 692 HPTQQGWAAAFS 701
           H + QGW + FS
Sbjct: 343 HISDQGWRSVFS 348

BLAST of Carg10471-RA vs. TrEMBL
Match: tr|A0A1S3CJ29|A0A1S3CJ29_CUCME (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.9e-127
Identity = 230/352 (65.34%), Postives = 263/352 (74.72%), Query Frame = 0

Query: 370 GRGSAGXXXXXXXXXXXXLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGK 429
           G+GSAG              HR R TKLFVFGDSY DTGNIL P S A QFPYGITFPGK
Sbjct: 3   GQGSAGGYYSNRHHR----SHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGK 62

Query: 430 PIGRFSDGRVLTDFAARHVGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLP 489
           P GRFSDGRVLTDFA +H+G+KSPIP+S+RN VG  R KE+G+NFAFGGTGVFDT  PLP
Sbjct: 63  PSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLP 122

Query: 490 NMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVN 549
           NMTTQID F+QLRH +S  +N D   SVALVS SGNDYSFY A N S QGLKPFI SVVN
Sbjct: 123 NMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVN 182

Query: 550 QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVK 609
           Q++++LKRI  LGVKKI VTGLGPLGCLP+FTAP S+K+CN+TINS VQFHNFLLK+AV 
Sbjct: 183 QVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVD 242

Query: 610 KLNQETKHSS---------------KVFIVDVYGAFMSIIEGRKRYNNGV--FKTPLKPC 669
           KLN++  +                   FI+DVY AF+S+I+GR     G+  FKTPLKPC
Sbjct: 243 KLNKQITNQQXXXXXXXXXXXXXXXXXFILDVYDAFLSLIQGRGSGRVGLLKFKTPLKPC 302

Query: 670 CHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKS 705
           C GV SGF+CGSVD +G K FV C D K+AFFWDSVHPTQ GW+ AFS+F S
Sbjct: 303 CFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTS 350

BLAST of Carg10471-RA vs. TrEMBL
Match: tr|A0A2U1N218|A0A2U1N218_ARTAN (SGNH hydrolase-type esterase domain-containing protein OS=Artemisia annua OX=35608 GN=CTI12_AA314900 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 3.9e-109
Identity = 227/612 (37.09%), Postives = 325/612 (53.10%), Query Frame = 0

Query: 96  VGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESL 155
           +G+KSP+P+ V      +   + G+NFAFGGTGVFDT    PN+TTQ  + Q+L   + +
Sbjct: 1   MGIKSPLPYRVTKYAPKK--LQAGINFAFGGTGVFDTGNFQPNLTTQTGYLQRLIK-DKV 60

Query: 156 STKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIA 215
            TK D + S A+V+VSGNDYS      G +Q L  FI  VVNQI +D+KRI +LG+ ++ 
Sbjct: 61  YTKRDLESSLAMVTVSGNDYSAFTAAGGTQQNLPAFITRVVNQITIDVKRIHDLGIPRVL 120

Query: 216 VTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMY 275
           V  L P+GCLP  T  SS+++C++T NS   FHN LL+ AV  LN  TK S+    LD++
Sbjct: 121 VNTLQPVGCLPQLTIQSSYQQCDQTQNSLASFHNQLLQVAVTTLNNNTKKST-FLPLDLF 180

Query: 276 GAFMSIIEGEKRYNNGV-FKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWD 335
            +F ++++ +      + F TPLKPCC    +  +CGSVD +GK ++ +C +P+  F+WD
Sbjct: 181 TSFNTVLKNKGDITGNLKFDTPLKPCCMATTNTSNCGSVDEKGKPLYTVCNEPESMFFWD 240

Query: 336 AVHPTQQGWAAAFGFCAYFIYFTISVSSLSNFFHLGRGSAGXXXXXXXXXXXXLRHRFRP 395
            VHPTQ GW A F                                               
Sbjct: 241 TVHPTQAGWRAVF----------------------------------------------- 300

Query: 396 TKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPI 455
                                                                +G+KSP+
Sbjct: 301 -----------------------------------------------------MGIKSPL 360

Query: 456 PYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQ 515
           PY +      +   + G+NFAFGGTGVFDT    PN+TTQ  + Q L   + V+T  D +
Sbjct: 361 PYRVTKYAPKK--LQAGINFAFGGTGVFDTGNFQPNLTTQTGYLQGLIK-NKVYTKRDLE 420

Query: 516 SSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPL 575
           SS+A+V+ SGNDYS + A   ++Q L  FI  VVNQI ++LKRI  LG+ ++ V  L P+
Sbjct: 421 SSLAMVTVSGNDYSAFTAAGGTQQNLPAFITRVVNQISIDLKRIHDLGIPRVLVNTLQPV 480

Query: 576 GCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSII 635
           GCLP  T  SSY++C++T NSL  FHN LL+ AV  LN  TK S+    +D++ +F +++
Sbjct: 481 GCLPQLTIQSSYQQCDQTQNSLASFHNQLLQVAVTTLNNNTKKST-FLPLDLFTSFNTVL 504

Query: 636 EGRKRYNNGV-FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQ 695
           + +      + F TPLKPCC    +  +CGSVD  GK ++  C + ++ FFWD+VHPTQ 
Sbjct: 541 KNKGDITGNLKFDTPLKPCCMATTNTSNCGSVDENGKPLYTVCNEPESMFFWDTVHPTQA 504

Query: 696 GWAAAFSAFKSF 706
           GW A   +  +F
Sbjct: 601 GWRAVSQSLGTF 504

BLAST of Carg10471-RA vs. TrEMBL
Match: tr|A0A1S3CH67|A0A1S3CH67_CUCME (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500887 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 1.3e-104
Identity = 203/346 (58.67%), Postives = 242/346 (69.94%), Query Frame = 0

Query: 366 FFHLGRGSAGXXXXXXXXXXXXLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGIT 425
           F H G+GS+                 F P KLFVFGDSY DTGNI    S A  FPYGIT
Sbjct: 17  FLHFGQGSSAV--------------HFHPLKLFVFGDSYVDTGNIHVNASSARNFPYGIT 76

Query: 426 FPGKPIGRFSDGRVLTDFAARHVGL-KSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDT 485
           FPG P GRFSDGRVLTDF A++VGL KSPIP+++  R G R+  + G+NF FGGTGVFDT
Sbjct: 77  FPGVPSGRFSDGRVLTDFLAKYVGLKKSPIPFTVWQRFG-RKGAKYGMNFGFGGTGVFDT 136

Query: 486 EYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFI 545
            +P PNMTTQI+FF+ L   +S+F++ D  SS+ALVS SGNDYSFYF  N S +G KPFI
Sbjct: 137 SFPFPNMTTQINFFRNL-IVNSIFSSYDIHSSIALVSPSGNDYSFYFTNNGSLEGFKPFI 196

Query: 546 MSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLL 605
            SVVNQI VNLKRI  LGVKK+ V GLGP+GC P  TAP S+KKCN+T++S+  FHN LL
Sbjct: 197 FSVVNQISVNLKRIYKLGVKKVVVLGLGPVGCYPKPTAP-SFKKCNKTMDSIAVFHNTLL 256

Query: 606 KEAVKKLNQETKHSS-KVFIVDVYGAFMSIIEGRKRYNNG-VFKTPLKPCCHGVGSGFSC 665
           K+AV+KLN ETK  S   FI+++Y   +SII+ ++    G  FKTPLKPCC GV S FSC
Sbjct: 257 KQAVEKLNYETKEGSPNFFILNMYDTVLSIIKNKENRKGGATFKTPLKPCCFGVNSNFSC 316

Query: 666 GSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKSFRNH 709
           GSVD  G KM+  C+    A FWD+VHPTQ GW A FS+FKS   H
Sbjct: 317 GSVDERGNKMYTLCKRPDLALFWDAVHPTQNGWFATFSSFKSTLKH 345

BLAST of Carg10471-RA vs. TrEMBL
Match: tr|A0A0A0KIM6|A0A0A0KIM6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452040 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 3.8e-104
Identity = 203/341 (59.53%), Postives = 240/341 (70.38%), Query Frame = 0

Query: 368 HLGRGSAGXXXXXXXXXXXXLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFP 427
           HLG+GS+             +   F P KLFVFGDSY DTGNI    S A  FPYGITFP
Sbjct: 19  HLGQGSSS-----------PVHAHFYPLKLFVFGDSYVDTGNINVNTSSARNFPYGITFP 78

Query: 428 GKPIGRFSDGRVLTDFAARHVGLK-SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEY 487
           G P GRFSDGRVLTDF A +VGLK SPIP++   + G +   + G+NFAFGGTGVFDT Y
Sbjct: 79  GFPSGRFSDGRVLTDFLANYVGLKRSPIPFTAWKKTGSKLRAKCGINFAFGGTGVFDTLY 138

Query: 488 PLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMS 547
           P PNMTTQI+FFQ L   +S+FT+ D  SS+ALVS SGNDYSFY A N S +G+KPF++S
Sbjct: 139 PFPNMTTQINFFQNL-IANSIFTSYDIHSSIALVSPSGNDYSFYLATNGSPEGVKPFVIS 198

Query: 548 VVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKE 607
           VVNQI VNLKRI  LGVKKI V GLGP+GC P  TAP S+KKCNET+NSL  FHN LLK+
Sbjct: 199 VVNQISVNLKRIYKLGVKKIVVVGLGPVGCYPSPTAP-SFKKCNETMNSLAVFHNTLLKQ 258

Query: 608 AVKKLNQETK--HSSKVFIVDVYGAFMSIIEGRKRYNNG-VFKTPLKPCCHGVGSGFSCG 667
           AV+KLN ETK   S   FI+++Y   +SII+ +     G  F+TPLKPCC GV S FSCG
Sbjct: 259 AVEKLNSETKLGGSPNFFILNMYDTVLSIIKNKGNPKVGATFQTPLKPCCFGVSSNFSCG 318

Query: 668 SVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKS 705
           SVD  G KM+  C+    A FWD+VHPTQ+GW A F++  S
Sbjct: 319 SVDEHGNKMYTLCKRPDLALFWDTVHPTQKGWFATFNSLIS 346

BLAST of Carg10471-RA vs. TrEMBL
Match: tr|A0A2P6RBN6|A0A2P6RBN6_ROSCH (Putative acetylajmaline esterase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0472731 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 4.3e-100
Identity = 182/313 (58.15%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           +RPTKLFVFGDSY+DTGN +  ++ + ++PYGITFPGKP GRFSDGRVLTDF A+ VG+K
Sbjct: 50  WRPTKLFVFGDSYSDTGNNMKSVTKSWKYPYGITFPGKPTGRFSDGRVLTDFLAKFVGVK 109

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNT 511
           SPIPY  RN VG++  K  G+NFA+GGTGVF+T    PNMTTQIDFFQQ    DSVF   
Sbjct: 110 SPIPYKFRN-VGVKHLK-YGMNFAYGGTGVFNTLVAAPNMTTQIDFFQQFIK-DSVFNAQ 169

Query: 512 DAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGL 571
           D  SSVAL++ +GNDY++Y A+N+S +G KPFI SVVNQ+ VNLKR+  LGVKKI VT L
Sbjct: 170 DLHSSVALITLAGNDYAYYLALNNSSEGFKPFITSVVNQLSVNLKRVYDLGVKKIVVTAL 229

Query: 572 GPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFM 631
            PLGCLP  TA  S++KCN T N LV FHN LL++A+ KLN E+K  S   ++D+Y +FM
Sbjct: 230 QPLGCLPGSTAELSFQKCNGTQNLLVGFHNLLLQQAIAKLNNESK--SSFIVLDLYTSFM 289

Query: 632 SIIEGRKRYNNGV-FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHP 691
           S+++ +  +   + F+ PL+PCC G+ S + CGSVD  G K +  C++ K+AFFWD  HP
Sbjct: 290 SVLKNKGDHLGSIKFENPLRPCCVGISSEYDCGSVDDSGAKKYTICDNPKSAFFWDGAHP 349

Query: 692 TQQGWAAAFSAFK 704
           TQQGW A +SA +
Sbjct: 350 TQQGWLAVYSALQ 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023543956.12.1e-19193.94GDSL esterase/lipase At5g03610-like, partial [Cucurbita pepo subsp. pepo][more]
XP_022925652.11.6e-18698.27GDSL esterase/lipase At5g03610-like isoform X3 [Cucurbita moschata][more]
XP_022925650.14.0e-18296.27GDSL esterase/lipase At5g03610-like isoform X1 [Cucurbita moschata][more]
XP_022925651.14.0e-18296.27GDSL esterase/lipase At5g03610-like isoform X2 [Cucurbita moschata][more]
XP_023544846.17.6e-18195.34GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G03610.13.7e-9755.41GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT3G09930.13.1e-8850.80GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT5G03600.13.3e-6945.17SGNH hydrolase-type esterase superfamily protein[more]
AT2G36325.17.5e-6643.91GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT5G03590.11.5e-3433.22GDSL-like Lipase/Acylhydrolase superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9LZS7|GDL71_ARATH6.7e-9655.41GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2... [more]
sp|Q9SF94|GDL50_ARATH5.7e-8750.80GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2... [more]
sp|Q9LZS8|GDL70_ARATH5.9e-6845.17GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3... [more]
sp|Q9LZS9|GDL69_ARATH2.5e-6645.98GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3... [more]
sp|Q8RWJ4|GDL45_ARATH1.4e-6443.91GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CJ29|A0A1S3CJ29_CUCME1.9e-12765.34GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=4... [more]
tr|A0A2U1N218|A0A2U1N218_ARTAN3.9e-10937.09SGNH hydrolase-type esterase domain-containing protein OS=Artemisia annua OX=356... [more]
tr|A0A1S3CH67|A0A1S3CH67_CUCME1.3e-10458.67GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500887 PE=4... [more]
tr|A0A0A0KIM6|A0A0A0KIM6_CUCSA3.8e-10459.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452040 PE=4 SV=1[more]
tr|A0A2P6RBN6|A0A2P6RBN6_ROSCH4.3e-10058.15Putative acetylajmaline esterase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g047... [more]
The following terms have been associated with this mRNA:
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: INTERPRO
TermDefinition
IPR035669SGNH_plant_lipase_like
IPR036514SGNH_hydro_sf
IPR001087GDSL
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016788 hydrolase activity, acting on ester bonds

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg10471Carg10471gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10471-RA.CDS.7Carg10471-RA.CDS.7CDS
Carg10471-RA.CDS.6Carg10471-RA.CDS.6CDS
Carg10471-RA.CDS.5Carg10471-RA.CDS.5CDS
Carg10471-RA.CDS.4Carg10471-RA.CDS.4CDS
Carg10471-RA.CDS.3Carg10471-RA.CDS.3CDS
Carg10471-RA.CDS.2Carg10471-RA.CDS.2CDS
Carg10471-RA.CDS.1Carg10471-RA.CDS.1CDS


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg10471-RA.exon.7Carg10471-RA.exon.7exon
Carg10471-RA.exon.6Carg10471-RA.exon.6exon
Carg10471-RA.exon.5Carg10471-RA.exon.5exon
Carg10471-RA.exon.4Carg10471-RA.exon.4exon
Carg10471-RA.exon.3Carg10471-RA.exon.3exon
Carg10471-RA.exon.2Carg10471-RA.exon.2exon
Carg10471-RA.exon.1Carg10471-RA.exon.1exon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg10471-RACarg10471-RA-proteinpolypeptide


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 45..344
e-value: 7.6E-13
score: 48.8
coord: 397..702
e-value: 1.2E-11
score: 44.9
IPR036514SGNH hydrolase superfamilyGENE3DG3DSA:3.40.50.1110coord: 28..347
e-value: 2.4E-31
score: 111.4
coord: 380..704
e-value: 3.9E-31
score: 110.7
NoneNo IPR availablePANTHERPTHR22835:SF324SUBFAMILY NOT NAMEDcoord: 388..704
coord: 34..347
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 388..704
coord: 34..347
NoneNo IPR availableSUPERFAMILYSSF52266SGNH hydrolasecoord: 326..347
coord: 42..61
coord: 121..285
NoneNo IPR availableSUPERFAMILYSSF52266SGNH hydrolasecoord: 678..702
coord: 394..413
coord: 451..637
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 397..702
e-value: 2.08929E-83
score: 267.944