Carg10471 (gene) Silver-seed gourd

NameCarg10471
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionGdsl esterase/lipase
LocationCucurbita_argyrosperma_scaffold_098 : 364329 .. 368992 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTGTTCTCCGCCTTCTCTTCTCTCTCCTCCTCCTCATCTCTCTCTTCTCAGGTTTGGATTCTGTGCTTATTTGAATTACTTCACAATTTCTGTTTGTTCTCTCTCTAATTTCTGCCATTTAGGACGAGTATCGGCTGGTGGCGTTTATAATCACCACCACCACCACCACCAGCGCCACCGTCTCCGGCCAACAAAGCTGTTTGTTTTCGGGGATTCCTATGTTGATACTGGCAACATTCTTATTCCCCTTTCCAGCGCTCAGCAATTTCCTTATGGAATCACTTTTCCAGGAAAACCCAGTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGGTAAACAAATCTCTGTCTCTGTTTTTGTCTCTCTCTGTCTCACCGATTTTCCGACCGTCCTGTTCTTACCAAACACAAATGGCTGTCACACAAGGCCTTAAAATGATCTTAATCTTGAAATGTAATGGAACGTATGGGAATAATTGAATGCAGCGAGATATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACATCGTTTCCTTTCCCAAACATGACAACCCAAATCGACTTCTTTCAACAGCTCCAACATGGCGAATCTCTGTCCACCAAAACAGACAACCAATTCTCCGCCGCACTCGTCTCTGTTTCCGGCAATGACTATTCCTTGGACATAATCACTAACGGCCCTAAACAGGTACCAATCAATTCCGTAATCTAATCCCAAGAGAATCATAAACTGAAATCCCGATGTGTTTTGCAGGGATTGAAACCGTTCATAAACACGGTAGTGAACCAGATCATCATGGATTTGAAACGCATTGGGGAGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGGTGTCTTCCGGCATTTACAGCTCCCTCGTCGTTCAAGAAATGCAATGAAACCATAAACTCGTTCGTGAAGTTTCACAACTTCTTGCTGAAGAAAGCAGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTGTTCTTGATATGTATGGGGCGTTCATGTCCATAATTGAAGGGGAGAAGAGATACAACAATGGAGTGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGTAAAGATCCCAAGAAAGCTTTCTATTGGGATGCAGTTCATCCTACACAACAAGGATGGGCTGCTGCGTTTTCCGCTTTAAAAGCCTTTCGCAACCATTTTTAAGTTGAATTAGTGGAGAATGATGTTATGGCATTGTGCCACACCCACCATAACAACATAACAACATGTACTAATAAACCTCTTCCATATCAAAATCAATTCACACATCTTTTCATCTAATTTTTTCGTGCAATATTAATGTTCGTTAAAATAAAATATGGGACAAGAACATACAAACCTCTAGACACGTTTTAAAATTATGAAAGAATATTCGAAAAAGCATTTAGGGAATCGGTTTGATTCTATCTTTGTCCCTTTGGTTTGAAGAAAGGAAGGATCCTAAAGAATTGATCTTTCTTTTAGTTGTTGAATCTCTCTTTGTTGATCCACCAGTGATATTCCAAATCTTGGTTACTAATAAAATCCAATTGAATAGAATCTATTACTTGAATGAAATTAGATCTCGTGGAATCATGTTGAATATTTGACAAGATATTCTGTGCCTTGCTAAAAAAAACGTTCCTTGTTTACCAACCACACATTATCTAATGAAATCTAATTGGGGCTAATGACGATACATATGGGACCAAAATGGACAAATATTTACTCGGGGTAGAATTTGATTGTTACAAATGGTATTAGAGCTGGGTAAGAGGGGCGGATTGTGAGAGGGAAACATAAATGAAGAATTTCATGGAGATGAGATGAAATGAGAGGCGATGTAATCTTCTTTCATCTTTAACTTACCGGGTGAATATCCCTAGTTGGAATAATTTTCTTATTTACTTCTTTGCAATAATTTTTTAAAAGGTTAAATTAATAAAAAAACACATATAAATTTTTATTTTATCGGACTTCCAAATTTTCAATAATAACCTTAAACTAAAAAAAATCTTAAAAAATAAATTTGGAAAATGTTTCTTTTAAAAATTTTAAAATACAAACTTTAAAAAAAAAAATGAGAGAGGAAGTCAGAGGTGAGAGAAAACACACGCTCAAGATGAGAGAGAAGAAGAAAAAAAAAGGGTGCAAAGCCCACGTGGCCCAACATGGGCCGCACATCCCATGCGGCCCAGCTCGGGCACTCGGCCCAACGGGCGTGTGGCCGACCCGACGACCCGCCCGGTAGTGGGCCGAACCGATAGCCCAACCCGGTTATGTCCAATTGGGCCAGTCTGACCTGGTTCTGTTCAATTGGACCAATTTTGTTCCTTTTGCGTAATTTTCTCGTTTCAACTCCGATTTGGAATTATTTGAGGATTTTAGAGTTATTAAGAATGCAAATCCGAACAAAAAGTGTCATACTTTTTAATTAGGTGGCACCTTAAAACCTTAGATTTAACTATGCTCGCAGTATGTATGAAAGTATGATATGAGAGTATGTCATAGAAATTTGAGTATGAAATGAGTTGGTTTAATGTATGTAAGAGTACGTTAAATGTTATGATGTACATTTATCAAGAAACTCGATTATATATGTTTGAGCATAGAATGAGTTGAGACAACACATATTGAATGTGATTATGTATGTTTACTGAAATGGCACTATTTTATGACTAGATATTCATATTTAGATTGCTATTATGTCTCTATATCATAAGTTTACGATGTATACAGTTATATGGGGATGATTAGGAGTTCGACAACTAACCTTTAATCGTGAACCTGACTCGATTGACAGGAGTTTGAATAACCATACCGTCATTATTGAACGGGTGGAAATTTGAAAACAGAAAAACAAACTAGAAACAAAAATTACCAAACAATTCATTTAAGGATGAAAAACAACTGTCCAAAGTGATGTCGTTTGTTTGTCGTTAAACATTACATGTATATATTGTCGAGTCTTCACCGATTGTATTCAAGCTTACAAGAACAAGCGGCTTCTCCGCCGGCCACCGCTTAACGTTGTGAAAGCATTCGATCCAGTAATCAAACACCTGCAACAATTTCCCTTTCACCTTATAATTCAATCATTATCAATATGATGGATTTTGTTCTCCGCCTTCTCTTCTCTCTCCTCCTTCTCATCTCTCTTTTCTCAGGTTTGGATTCTGTGCTTATTTCATTTACTTCACAATTTCTGTTTCTTCTCTCTCTAATTTCTTCCATTTAGGAAGAGGATCGGCTGGTGGCGTTTACAATCACCACCACCACCGCCGCCGCCATCTCCGCCACCGTTTCCGGCCAACAAAGCTGTTTGTTTTTGGGGATTCCTATGCTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGCTCTGCAATTTCCTTATGGAATTACTTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGGTAAACAAATCTCTGTCTCTGTCTCTGTTTCTGTCTCTCTCTGTTTCGCCGATTTTCCGACCGTCCTGTTCTTACTAAAAAAAAATGGCTGTCAAACGAGGCCTTAAAATCATCTTAATCTCGAAATTTAATGGAACGTATGGGAATAATTGAATGCAGCGAGACATGTCGGGTTGAAATCTCCGATCCCATACAGTCTCCGAAACAGAGTTGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTCTTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCCCAATCCTCTGTCGCACTCGTCTCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGTATCAATCAATTCCGCAATCTAACCCCAAGAGAATCACAAACTGAAATTCCGATGTGTTTTACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACCATAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTTTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGTGTTCAAGACGCCCTTGAAGCCGTGTTGTCATGGAGTGGGGAGTGGATTTAGTTGCGGGAGTGTGGACGGAGAAGGGAAGAAGATGTTTGTGTTCTGTGAGGATCAGAAAGCAGCTTTCTTTTGGGATTCGGTGCATCCTACGCAACAAGGATGGGCCGCTGCTTTCTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

mRNA sequence

ATGGATTCTGTTCTCCGCCTTCTCTTCTCTCTCCTCCTCCTCATCTCTCTCTTCTCAGGACGAGTATCGGCTGGTGGCGTTTATAATCACCACCACCACCACCACCAGCGCCACCGTCTCCGGCCAACAAAGCTGTTTGTTTTCGGGGATTCCTATGTTGATACTGGCAACATTCTTATTCCCCTTTCCAGCGCTCAGCAATTTCCTTATGGAATCACTTTTCCAGGAAAACCCAGTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGCGAGATATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACATCGTTTCCTTTCCCAAACATGACAACCCAAATCGACTTCTTTCAACAGCTCCAACATGGCGAATCTCTGTCCACCAAAACAGACAACCAATTCTCCGCCGCACTCGTCTCTGTTTCCGGCAATGACTATTCCTTGGACATAATCACTAACGGCCCTAAACAGGGATTGAAACCGTTCATAAACACGGTAGTGAACCAGATCATCATGGATTTGAAACGCATTGGGGAGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGGTGTCTTCCGGCATTTACAGCTCCCTCGTCGTTCAAGAAATGCAATGAAACCATAAACTCGTTCGTGAAGTTTCACAACTTCTTGCTGAAGAAAGCAGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTGTTCTTGATATGTATGGGGCGTTCATGTCCATAATTGAAGGGGAGAAGAGATACAACAATGGAGTGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGTAAAGATCCCAAGAAAGCTTTCTATTGGGATGCAGTTCATCCTACACAACAAGGATGGGCTGCTGCGTTTGGATTCTGTGCTTATTTCATTTACTTCACAATTTCTGTTTCTTCTCTCTCTAATTTCTTCCATTTAGGAAGAGGATCGGCTGGTGGCGTTTACAATCACCACCACCACCGCCGCCGCCATCTCCGCCACCGTTTCCGGCCAACAAAGCTGTTTGTTTTTGGGGATTCCTATGCTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGCTCTGCAATTTCCTTATGGAATTACTTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGCGAGACATGTCGGGTTGAAATCTCCGATCCCATACAGTCTCCGAAACAGAGTTGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTCTTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCCCAATCCTCTGTCGCACTCGTCTCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACCATAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTTTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGTGTTCAAGACGCCCTTGAAGCCGTGTTGTCATGGAGTGGGGAGTGGATTTAGTTGCGGGAGTGTGGACGGAGAAGGGAAGAAGATGTTTGTGTTCTGTGAGGATCAGAAAGCAGCTTTCTTTTGGGATTCGGTGCATCCTACGCAACAAGGATGGGCCGCTGCTTTCTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

Coding sequence (CDS)

ATGGATTCTGTTCTCCGCCTTCTCTTCTCTCTCCTCCTCCTCATCTCTCTCTTCTCAGGACGAGTATCGGCTGGTGGCGTTTATAATCACCACCACCACCACCACCAGCGCCACCGTCTCCGGCCAACAAAGCTGTTTGTTTTCGGGGATTCCTATGTTGATACTGGCAACATTCTTATTCCCCTTTCCAGCGCTCAGCAATTTCCTTATGGAATCACTTTTCCAGGAAAACCCAGTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGCGAGATATGTCGGGTTGAAATCTCCGATCCCATTCAGTGTCCGAAACAGAGTCGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACATCGTTTCCTTTCCCAAACATGACAACCCAAATCGACTTCTTTCAACAGCTCCAACATGGCGAATCTCTGTCCACCAAAACAGACAACCAATTCTCCGCCGCACTCGTCTCTGTTTCCGGCAATGACTATTCCTTGGACATAATCACTAACGGCCCTAAACAGGGATTGAAACCGTTCATAAACACGGTAGTGAACCAGATCATCATGGATTTGAAACGCATTGGGGAGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGGTGTCTTCCGGCATTTACAGCTCCCTCGTCGTTCAAGAAATGCAATGAAACCATAAACTCGTTCGTGAAGTTTCACAACTTCTTGCTGAAGAAAGCAGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTGTTCTTGATATGTATGGGGCGTTCATGTCCATAATTGAAGGGGAGAAGAGATACAACAATGGAGTGTTCAAGACGCCATTGAAGCCGTGTTGTTTTGGAGTGAAGAGTGGATTTGATTGTGGGAGTGTGGATGGAGAAGGGAAGAAGATGTTTGTGTTATGTAAAGATCCCAAGAAAGCTTTCTATTGGGATGCAGTTCATCCTACACAACAAGGATGGGCTGCTGCGTTTGGATTCTGTGCTTATTTCATTTACTTCACAATTTCTGTTTCTTCTCTCTCTAATTTCTTCCATTTAGGAAGAGGATCGGCTGGTGGCGTTTACAATCACCACCACCACCGCCGCCGCCATCTCCGCCACCGTTTCCGGCCAACAAAGCTGTTTGTTTTTGGGGATTCCTATGCTGATACCGGCAACATTCTTTTTCCCATTTCCCCCGCTCTGCAATTTCCTTATGGAATTACTTTTCCAGGAAAACCCATTGGCCGTTTCTCCGATGGTCGTGTTCTCACTGATTTTGCAGCGAGACATGTCGGGTTGAAATCTCCGATCCCATACAGTCTCCGAAACAGAGTTGGGTTGAGAAGATTCAAAGAAACAGGAGTAAACTTTGCATTTGGGGGAACCGGCGTCTTCGACACAGAGTATCCTTTGCCAAACATGACAACCCAGATCGACTTCTTTCAACAGCTCCGACATGGCGATTCCGTATTCACCAATACAGACGCCCAATCCTCTGTCGCACTCGTCTCTGCTTCCGGCAACGACTATTCCTTTTACTTCGCCATGAACAGCTCTAGACAGGGATTGAAACCGTTCATAATGTCGGTAGTGAATCAGATCATCGTGAATTTGAAACGCATTGGGGGGTTGGGAGTGAAGAAGATAGCGGTGACAGGGCTAGGCCCATTGGGATGTCTTCCGGTATTTACAGCGCCCTCATCATACAAGAAATGCAATGAAACCATAAACTCGTTGGTGCAGTTTCACAACTTTTTGTTGAAGGAAGCTGTGAAGAAGCTGAACCAAGAAACGAAGCATTCTTCGAAGGTGTTTATTGTTGATGTTTATGGGGCGTTCATGTCCATAATTGAAGGGCGGAAGAGATACAACAATGGAGTGTTCAAGACGCCCTTGAAGCCGTGTTGTCATGGAGTGGGGAGTGGATTTAGTTGCGGGAGTGTGGACGGAGAAGGGAAGAAGATGTTTGTGTTCTGTGAGGATCAGAAAGCAGCTTTCTTTTGGGATTCGGTGCATCCTACGCAACAAGGATGGGCCGCTGCTTTCTCTGCTTTTAAAAGCTTTCGCAACCATTTTTAA

Protein sequence

MDSVLRLLFSLLLLISLFSGRVSAGGVYNHHHHHHQRHRLRPTKLFVFGDSYVDTGNILIPLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAFGFCAYFIYFTISVSSLSNFFHLGRGSAGGVYNHHHHRRRHLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKSFRNHF
BLAST of Carg10471 vs. NCBI nr
Match: XP_023543956.1 (GDSL esterase/lipase At5g03610-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678.7 bits (1750), Expect = 2.1e-191
Identity = 341/363 (93.94%), Postives = 347/363 (95.59%), Query Frame = 0

Query: 347 FGFCAYFIYFTISVSSLSNFFHLGRGSAGXXXXXXXXXXXXLRHRFRPTKLFVFGDSYAD 406
           FGFCAYFIYFTISV SLSNFFHLGRGSAG   XXXXXXXXX    FRPTKLFVFGDSYAD
Sbjct: 22  FGFCAYFIYFTISVCSLSNFFHLGRGSAG---XXXXXXXXXXXXXFRPTKLFVFGDSYAD 81

Query: 407 TGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPYSLRNRVGLRR 466
           TGNILFPISP LQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIP+S+RNRVG RR
Sbjct: 82  TGNILFPISPGLQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIPFSVRNRVGSRR 141

Query: 467 FKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGND 526
           FKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGND
Sbjct: 142 FKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGND 201

Query: 527 YSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSY 586
           YSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSY
Sbjct: 202 YSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSY 261

Query: 587 KKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSIIEGRKRYNNGVFK 646
           KKCNET+NSLVQFHNFLLKE VKKLNQETKHSSKVFIVDVYGAFMSII+GRKRYNNG FK
Sbjct: 262 KKCNETVNSLVQFHNFLLKEGVKKLNQETKHSSKVFIVDVYGAFMSIIQGRKRYNNGEFK 321

Query: 647 TPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKSFR 706
           TPLKPCC GV SGF CGSVDGEGKKMFV C+D+KAAFFWDSVHPTQQGWAAAFSAFKSFR
Sbjct: 322 TPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDRKAAFFWDSVHPTQQGWAAAFSAFKSFR 381

Query: 707 NHF 710
           NHF
Sbjct: 382 NHF 381

BLAST of Carg10471 vs. NCBI nr
Match: XP_022925652.1 (GDSL esterase/lipase At5g03610-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 662.5 bits (1708), Expect = 1.6e-186
Identity = 341/347 (98.27%), Postives = 344/347 (99.14%), Query Frame = 0

Query: 1   MDSVLRLLFSLLLLISLFSGRVSAXXXXXXXXXXXXXXRLRPTKLFVFGDSYVDTGNILI 60
           MD VLRLLFSLLLLISLFSGRVSA  XXXXXXXXXXXXRLRPTKLFVFGDSYVDTGNILI
Sbjct: 1   MDFVLRLLFSLLLLISLFSGRVSA-GXXXXXXXXXXXXRLRPTKLFVFGDSYVDTGNILI 60

Query: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFSVRNRVGLRRFKETGV 120
           PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPF+V+NRVGLRRFKETGV
Sbjct: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGV 120

Query: 121 NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII 180
           NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII
Sbjct: 121 NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII 180

Query: 181 TNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET 240
           TNGPKQGLKPFINTVVNQII+DLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET
Sbjct: 181 TNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET 240

Query: 241 INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC 300
           INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC
Sbjct: 241 INSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMYGAFMSIIEGEKRYNNGVFKTPLKPC 300

Query: 301 CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF 348
           CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF
Sbjct: 301 CFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF 346

BLAST of Carg10471 vs. NCBI nr
Match: XP_022925650.1 (GDSL esterase/lipase At5g03610-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 647.9 bits (1670), Expect = 4.0e-182
Identity = 310/322 (96.27%), Postives = 318/322 (98.76%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH
Sbjct: 39  LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 98

Query: 448 VGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSV 507
           VGLKSPIP+S+RNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDS+
Sbjct: 99  VGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSI 158

Query: 508 FTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA 567
           FTNTDAQSSVALVSASGNDY+FYFAMNSSRQGLKPFIMSVV+QIIVNLKRIGGLGVKKIA
Sbjct: 159 FTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIA 218

Query: 568 VTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVY 627
           VTGLGPLGCLPVFTAPSSYK+CNETINSLVQFHN LLKEAVKKLNQETKHSSKVF+VDVY
Sbjct: 219 VTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVY 278

Query: 628 GAFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 687
           GAFMSIIEGRKRYNNG FKTPLKPCC+GVGSGFSCGSVDGEGKKMFV CEDQKAAFFWDS
Sbjct: 279 GAFMSIIEGRKRYNNGEFKTPLKPCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDS 338

Query: 688 VHPTQQGWAAAFSAFKSFRNHF 710
           VHPTQQGWAAA SAFKSFRNHF
Sbjct: 339 VHPTQQGWAAAISAFKSFRNHF 360

BLAST of Carg10471 vs. NCBI nr
Match: XP_022925651.1 (GDSL esterase/lipase At5g03610-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 647.9 bits (1670), Expect = 4.0e-182
Identity = 310/322 (96.27%), Postives = 318/322 (98.76%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH
Sbjct: 38  LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 97

Query: 448 VGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSV 507
           VGLKSPIP+S+RNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDS+
Sbjct: 98  VGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSI 157

Query: 508 FTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA 567
           FTNTDAQSSVALVSASGNDY+FYFAMNSSRQGLKPFIMSVV+QIIVNLKRIGGLGVKKIA
Sbjct: 158 FTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIMSVVSQIIVNLKRIGGLGVKKIA 217

Query: 568 VTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVY 627
           VTGLGPLGCLPVFTAPSSYK+CNETINSLVQFHN LLKEAVKKLNQETKHSSKVF+VDVY
Sbjct: 218 VTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLKEAVKKLNQETKHSSKVFVVDVY 277

Query: 628 GAFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 687
           GAFMSIIEGRKRYNNG FKTPLKPCC+GVGSGFSCGSVDGEGKKMFV CEDQKAAFFWDS
Sbjct: 278 GAFMSIIEGRKRYNNGEFKTPLKPCCYGVGSGFSCGSVDGEGKKMFVLCEDQKAAFFWDS 337

Query: 688 VHPTQQGWAAAFSAFKSFRNHF 710
           VHPTQQGWAAA SAFKSFRNHF
Sbjct: 338 VHPTQQGWAAAISAFKSFRNHF 359

BLAST of Carg10471 vs. NCBI nr
Match: XP_023544846.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 643.7 bits (1659), Expect = 7.6e-181
Identity = 307/322 (95.34%), Postives = 314/322 (97.52%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH
Sbjct: 38  LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 97

Query: 448 VGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSV 507
           VGLKSPIP+S+RNRVGLRR KETGVNFAFGGTGVFDTEYP PNMTTQIDFFQQLRHGDS+
Sbjct: 98  VGLKSPIPFSVRNRVGLRRLKETGVNFAFGGTGVFDTEYPFPNMTTQIDFFQQLRHGDSI 157

Query: 508 FTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA 567
           FTN DAQSSVALVSASGNDYSFYFAMNS+RQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA
Sbjct: 158 FTNKDAQSSVALVSASGNDYSFYFAMNSTRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIA 217

Query: 568 VTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVY 627
           VTGLGPLGCLPVFTAPSSYK+CNET+NSLVQFHN LLKE VKKLNQETKHSSKVFIVDVY
Sbjct: 218 VTGLGPLGCLPVFTAPSSYKQCNETVNSLVQFHNLLLKEGVKKLNQETKHSSKVFIVDVY 277

Query: 628 GAFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 687
           GAFMSIIEGRKRYNNG FKTPLKPCCHGVGSGFSCGSVDGEGKKM+V CED KAAFFWDS
Sbjct: 278 GAFMSIIEGRKRYNNGEFKTPLKPCCHGVGSGFSCGSVDGEGKKMYVLCEDHKAAFFWDS 337

Query: 688 VHPTQQGWAAAFSAFKSFRNHF 710
           VHPTQQGWAAAFSAFKSFRNHF
Sbjct: 338 VHPTQQGWAAAFSAFKSFRNHF 359

BLAST of Carg10471 vs. TAIR10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 353.2 bits (905), Expect = 3.7e-97
Identity = 174/314 (55.41%), Postives = 225/314 (71.66%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           FRPTKLFVFGDSYADTGNI    S + +FPYGITFPGKP GRFSDGRV TDF A+ VG+K
Sbjct: 39  FRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIK 98

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNT 511
           SPIPY  ++  G +R  + G+NFA+GGTGVF+T+ PLPNMTTQID FQ +     ++   
Sbjct: 99  SPIPYFWKDYAGKKRL-QYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPP 158

Query: 512 DAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGL 571
           +  SSVALVS +GNDYS + A+N        FI  VV+Q  VNL+RI  LGVKKIAV  L
Sbjct: 159 ELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSL 218

Query: 572 GPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFM 631
            PLGCLP FT  +S+++CNET N+LV  HN LL++ V KLN ETK S+ + I+D+Y AF+
Sbjct: 219 QPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQSTFI-ILDLYNAFL 278

Query: 632 SIIEGRKRYNNGV--FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVH 691
           ++ +  K  N G   F++PLKPCC GV   ++CGSVD +G K ++ C++ K AFFWD +H
Sbjct: 279 TVFK-NKGSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLH 338

Query: 692 PTQQGWAAAFSAFK 704
           PT++GW + +S  +
Sbjct: 339 PTEEGWRSVYSVLR 349

BLAST of Carg10471 vs. TAIR10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 323.6 bits (828), Expect = 3.1e-88
Identity = 159/313 (50.80%), Postives = 217/313 (69.33%), Query Frame = 0

Query: 391 RFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGL 450
           R RP +LFVFGDSYADTGNI   +S + + PYGITFP KP GRFSDGRV TDF AR++G+
Sbjct: 34  RLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGI 93

Query: 451 KSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDT-EYPLPNMTTQIDFFQQLRHGDSVFT 510
           KSPIPY+ ++  G  R    G+N+A+GGTGVF T + PLPNMTTQID+FQ++    ++++
Sbjct: 94  KSPIPYTWKDYAGKERLL-YGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYS 153

Query: 511 NTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVT 570
            +D  SS+ALVS +GNDY+ + A+      L  F+  VV+QI VN  RI  LGV KI + 
Sbjct: 154 PSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIP 213

Query: 571 GLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGA 630
            + PLGCLP  T  +S+++CN T N+    HN+LL +A+ +LN ETK S+ V ++D Y A
Sbjct: 214 SMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNETKPSTFV-VLDHYNA 273

Query: 631 FMSIIEGRKRYNNGV--FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 690
           F+++ +  K    GV  F  PLKPCC GV S + C +VD +G+K ++ CED KAAFFWD 
Sbjct: 274 FLTVFK-NKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDI 333

Query: 691 VHPTQQGWAAAFS 701
            HP+++GW + +S
Sbjct: 334 FHPSEEGWRSVYS 343

BLAST of Carg10471 vs. TAIR10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 260.4 bits (664), Expect = 3.3e-69
Identity = 145/321 (45.17%), Postives = 199/321 (61.99%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LR R  P KLFVFGDSYADTGN     + A   PYGITFPGKP GR+ DG + TDF  + 
Sbjct: 7   LRPRKVP-KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKV 66

Query: 448 VGLKSPIPYSLRNR-VGLRRFKETGVNFAFGGTGVFDT--EYPLPNMTTQIDFFQQLRHG 507
           +G +SP  Y    R  GL+R    G+NFAFGG+ + D+    P PN+T Q++F   L   
Sbjct: 67  LGAESPYLYRTHGRDKGLKR----GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLA 126

Query: 508 DSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVK 567
             V+ +    S V+L+S +G DY +Y   N    GLK  +  VV+ + VN+  +GGL  K
Sbjct: 127 GRVYGDI-TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFK 186

Query: 568 KIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQET---KHSSKV 627
           KIAVT L P+GCLP +T+ SS+K CNE+ ++LV+ HN LLK+ V KLN+++   K     
Sbjct: 187 KIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF 246

Query: 628 FIVDVYGAFMSII--EGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQ 687
           FI+D++ AFM+++  +G KR     FK P+K CC G      CG    +G K++  C+D 
Sbjct: 247 FIIDIHNAFMTVMKNKGSKR-----FKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDP 306

Query: 688 KAAFFWDSVHPTQQGWAAAFS 701
           K+ FFWD+VHPTQ+GW + +S
Sbjct: 307 KSFFFWDAVHPTQEGWRSIYS 309

BLAST of Carg10471 vs. TAIR10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 249.2 bits (635), Expect = 7.5e-66
Identity = 137/312 (43.91%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           F+PTKLFVFGDSYADTGN  F I P+ +FP GITFPG P GRFSDGRV TD+ A+++G++
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPL-PNMTTQIDFFQQLRHGDSVFTN 511
           +PI Y        R   + G+NFA+GG G F+T + L P  + QID F+QL    +V++ 
Sbjct: 103 TPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSP 162

Query: 512 TDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTG 571
            D  SSVA  S  GNDY  Y   N S +G       VV QI++++KRI  LGV+K+ V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 572 LGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLN-QETKHSSKVFI-VDVYG 631
             P  CLP    P   K C +T ++    HN LL++ + KLN +E  ++ K F+ +D+Y 
Sbjct: 223 SPPQKCLPKLVTP---KGC-DTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 282

Query: 632 AFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSV 691
           AF++I + +       F  P K CC     G  CG     GKK++  C+D K+ FFWD+V
Sbjct: 283 AFVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWDNV 342

Query: 692 HPTQQGWAAAFS 701
           H + QGW + FS
Sbjct: 343 HISDQGWRSVFS 348

BLAST of Carg10471 vs. TAIR10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein)

HSP 1 Score: 145.2 bits (365), Expect = 1.5e-34
Identity = 97/292 (33.22%), Postives = 132/292 (45.21%), Query Frame = 0

Query: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFSVRNRVGLRRFKETGV 120
           P + + + PYGITFPGKPSGR+SDG   TDF                             
Sbjct: 3   PTALSWKLPYGITFPGKPSGRYSDGLTATDFL---------------------------- 62

Query: 121 NFAFGGTGVFDTSFP-FPNMTTQIDFFQQLQHGESLST-KTDNQFSAALVSVSGNDYSLD 180
                G+ VFD+     PN++TQ+ F   L     + T   D   S AL+S SG DY   
Sbjct: 63  -----GSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGF 122

Query: 181 IITNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCN 240
           I  N        F+  +V  I                                     CN
Sbjct: 123 IDQNPNMAAYPAFVEFIVEDI------------------------------------HCN 182

Query: 241 ETINSFVKFHNFLLKKAVKKLNQETKHSSK---VFVLDMYGAFMSIIEGEKRYNNGVFKT 300
           E+ +  V+ HN  LKKAV KLN+E K  +K     ++D++ AFM+I+E   +  N  FK+
Sbjct: 183 ESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILE---KKGNKRFKS 217

Query: 301 PLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAAF 348
           PLKPCC G     DC  +D +G K + LC DPK AF+WD ++PTQ+GW + +
Sbjct: 243 PLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIY 217

BLAST of Carg10471 vs. Swiss-Prot
Match: sp|Q9LZS7|GDL71_ARATH (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.7e-96
Identity = 174/314 (55.41%), Postives = 225/314 (71.66%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           FRPTKLFVFGDSYADTGNI    S + +FPYGITFPGKP GRFSDGRV TDF A+ VG+K
Sbjct: 39  FRPTKLFVFGDSYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIK 98

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNT 511
           SPIPY  ++  G +R  + G+NFA+GGTGVF+T+ PLPNMTTQID FQ +     ++   
Sbjct: 99  SPIPYFWKDYAGKKRL-QYGMNFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPP 158

Query: 512 DAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGL 571
           +  SSVALVS +GNDYS + A+N        FI  VV+Q  VNL+RI  LGVKKIAV  L
Sbjct: 159 ELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSL 218

Query: 572 GPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFM 631
            PLGCLP FT  +S+++CNET N+LV  HN LL++ V KLN ETK S+ + I+D+Y AF+
Sbjct: 219 QPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVAKLNNETKQSTFI-ILDLYNAFL 278

Query: 632 SIIEGRKRYNNGV--FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVH 691
           ++ +  K  N G   F++PLKPCC GV   ++CGSVD +G K ++ C++ K AFFWD +H
Sbjct: 279 TVFK-NKGSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLH 338

Query: 692 PTQQGWAAAFSAFK 704
           PT++GW + +S  +
Sbjct: 339 PTEEGWRSVYSVLR 349

BLAST of Carg10471 vs. Swiss-Prot
Match: sp|Q9SF94|GDL50_ARATH (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.7e-87
Identity = 159/313 (50.80%), Postives = 217/313 (69.33%), Query Frame = 0

Query: 391 RFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGL 450
           R RP +LFVFGDSYADTGNI   +S + + PYGITFP KP GRFSDGRV TDF AR++G+
Sbjct: 34  RLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFLARYLGI 93

Query: 451 KSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDT-EYPLPNMTTQIDFFQQLRHGDSVFT 510
           KSPIPY+ ++  G  R    G+N+A+GGTGVF T + PLPNMTTQID+FQ++    ++++
Sbjct: 94  KSPIPYTWKDYAGKERLL-YGMNYAYGGTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYS 153

Query: 511 NTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVT 570
            +D  SS+ALVS +GNDY+ + A+      L  F+  VV+QI VN  RI  LGV KI + 
Sbjct: 154 PSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIP 213

Query: 571 GLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGA 630
            + PLGCLP  T  +S+++CN T N+    HN+LL +A+ +LN ETK S+ V ++D Y A
Sbjct: 214 SMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNETKPSTFV-VLDHYNA 273

Query: 631 FMSIIEGRKRYNNGV--FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDS 690
           F+++ +  K    GV  F  PLKPCC GV S + C +VD +G+K ++ CED KAAFFWD 
Sbjct: 274 FLTVFK-NKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDI 333

Query: 691 VHPTQQGWAAAFS 701
            HP+++GW + +S
Sbjct: 334 FHPSEEGWRSVYS 343

BLAST of Carg10471 vs. Swiss-Prot
Match: sp|Q9LZS8|GDL70_ARATH (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 5.9e-68
Identity = 145/321 (45.17%), Postives = 199/321 (61.99%), Query Frame = 0

Query: 388 LRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARH 447
           LR R  P KLFVFGDSYADTGN     + A   PYGITFPGKP GR+ DG + TDF  + 
Sbjct: 7   LRPRKVP-KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKV 66

Query: 448 VGLKSPIPYSLRNR-VGLRRFKETGVNFAFGGTGVFDT--EYPLPNMTTQIDFFQQLRHG 507
           +G +SP  Y    R  GL+R    G+NFAFGG+ + D+    P PN+T Q++F   L   
Sbjct: 67  LGAESPYLYRTHGRDKGLKR----GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLA 126

Query: 508 DSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVK 567
             V+ +    S V+L+S +G DY +Y   N    GLK  +  VV+ + VN+  +GGL  K
Sbjct: 127 GRVYGDI-TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFK 186

Query: 568 KIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQET---KHSSKV 627
           KIAVT L P+GCLP +T+ SS+K CNE+ ++LV+ HN LLK+ V KLN+++   K     
Sbjct: 187 KIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHF 246

Query: 628 FIVDVYGAFMSII--EGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQ 687
           FI+D++ AFM+++  +G KR     FK P+K CC G      CG    +G K++  C+D 
Sbjct: 247 FIIDIHNAFMTVMKNKGSKR-----FKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDP 306

Query: 688 KAAFFWDSVHPTQQGWAAAFS 701
           K+ FFWD+VHPTQ+GW + +S
Sbjct: 307 KSFFFWDAVHPTQEGWRSIYS 309

BLAST of Carg10471 vs. Swiss-Prot
Match: sp|Q9LZS9|GDL69_ARATH (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 255.0 bits (650), Expect = 2.5e-66
Identity = 143/311 (45.98%), Postives = 192/311 (61.74%), Query Frame = 0

Query: 396 KLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPIP 455
           KLFVFG+SYADTGN + P + + + PYGITFPGKP GR+SDG   TDF A+ +G K  +P
Sbjct: 34  KLFVFGNSYADTGN-MKPTALSWKLPYGITFPGKPSGRYSDGLTATDFLAKQLGAK--LP 93

Query: 456 YSLRNRVGLRRFK-ETGVNFAFGGTGVFDTEYP-LPNMTTQIDFFQQLRHGDSVFT-NTD 515
           Y  R   G ++ K   G+NFAFGG+ VFD+     PN++TQ+ F   L     V+T + D
Sbjct: 94  YLWRTH-GKKKVKLNRGMNFAFGGSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGD 153

Query: 516 AQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLG 575
             SS AL+S SG DY  +   N +      F+  +V  I  +L  + GL  K IAVT L 
Sbjct: 154 LASSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLH 213

Query: 576 PLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSK---VFIVDVYGA 635
           PLGCLP  T  SS++ CNE+ + LV+ HN  LK+AV KLN+E K  +K     IVD++ A
Sbjct: 214 PLGCLPRVTVASSFRSCNESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKA 273

Query: 636 FMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVH 695
           FM+I+E   +  N  FK+PLKPCC G      C  +D +G K +  C D K+AFFWD ++
Sbjct: 274 FMTILE---KKGNKRFKSPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEIN 332

Query: 696 PTQQGWAAAFS 701
           PTQ+GW + +S
Sbjct: 334 PTQEGWRSIYS 332

BLAST of Carg10471 vs. Swiss-Prot
Match: sp|Q8RWJ4|GDL45_ARATH (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 249.2 bits (635), Expect = 1.4e-64
Identity = 137/312 (43.91%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           F+PTKLFVFGDSYADTGN  F I P+ +FP GITFPG P GRFSDGRV TD+ A+++G++
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPL-PNMTTQIDFFQQLRHGDSVFTN 511
           +PI Y        R   + G+NFA+GG G F+T + L P  + QID F+QL    +V++ 
Sbjct: 103 TPITYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLM-RNVYSP 162

Query: 512 TDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTG 571
            D  SSVA  S  GNDY  Y   N S +G       VV QI++++KRI  LGV+K+ V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 572 LGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLN-QETKHSSKVFI-VDVYG 631
             P  CLP    P   K C +T ++    HN LL++ + KLN +E  ++ K F+ +D+Y 
Sbjct: 223 SPPQKCLPKLVTP---KGC-DTNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 282

Query: 632 AFMSIIEGRKRYNNGVFKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSV 691
           AF++I + +       F  P K CC     G  CG     GKK++  C+D K+ FFWD+V
Sbjct: 283 AFVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWDNV 342

Query: 692 HPTQQGWAAAFS 701
           H + QGW + FS
Sbjct: 343 HISDQGWRSVFS 348

BLAST of Carg10471 vs. TrEMBL
Match: tr|A0A1S3CJ29|A0A1S3CJ29_CUCME (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.9e-127
Identity = 230/352 (65.34%), Postives = 263/352 (74.72%), Query Frame = 0

Query: 370 GRGSAGXXXXXXXXXXXXLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGK 429
           G+GSAG              HR R TKLFVFGDSY DTGNIL P S A QFPYGITFPGK
Sbjct: 3   GQGSAGGYYSNRHHR----SHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGK 62

Query: 430 PIGRFSDGRVLTDFAARHVGLKSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLP 489
           P GRFSDGRVLTDFA +H+G+KSPIP+S+RN VG  R KE+G+NFAFGGTGVFDT  PLP
Sbjct: 63  PSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLP 122

Query: 490 NMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVN 549
           NMTTQID F+QLRH +S  +N D   SVALVS SGNDYSFY A N S QGLKPFI SVVN
Sbjct: 123 NMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVN 182

Query: 550 QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVK 609
           Q++++LKRI  LGVKKI VTGLGPLGCLP+FTAP S+K+CN+TINS VQFHNFLLK+AV 
Sbjct: 183 QVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVD 242

Query: 610 KLNQETKHSS---------------KVFIVDVYGAFMSIIEGRKRYNNGV--FKTPLKPC 669
           KLN++  +                   FI+DVY AF+S+I+GR     G+  FKTPLKPC
Sbjct: 243 KLNKQITNQQXXXXXXXXXXXXXXXXXFILDVYDAFLSLIQGRGSGRVGLLKFKTPLKPC 302

Query: 670 CHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKS 705
           C GV SGF+CGSVD +G K FV C D K+AFFWDSVHPTQ GW+ AFS+F S
Sbjct: 303 CFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTS 350

BLAST of Carg10471 vs. TrEMBL
Match: tr|A0A2U1N218|A0A2U1N218_ARTAN (SGNH hydrolase-type esterase domain-containing protein OS=Artemisia annua OX=35608 GN=CTI12_AA314900 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 3.9e-109
Identity = 227/612 (37.09%), Postives = 325/612 (53.10%), Query Frame = 0

Query: 96  VGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESL 155
           +G+KSP+P+ V      +   + G+NFAFGGTGVFDT    PN+TTQ  + Q+L   + +
Sbjct: 1   MGIKSPLPYRVTKYAPKK--LQAGINFAFGGTGVFDTGNFQPNLTTQTGYLQRLIK-DKV 60

Query: 156 STKTDNQFSAALVSVSGNDYSLDIITNGPKQGLKPFINTVVNQIIMDLKRIGELGVKKIA 215
            TK D + S A+V+VSGNDYS      G +Q L  FI  VVNQI +D+KRI +LG+ ++ 
Sbjct: 61  YTKRDLESSLAMVTVSGNDYSAFTAAGGTQQNLPAFITRVVNQITIDVKRIHDLGIPRVL 120

Query: 216 VTGLGPLGCLPAFTAPSSFKKCNETINSFVKFHNFLLKKAVKKLNQETKHSSKVFVLDMY 275
           V  L P+GCLP  T  SS+++C++T NS   FHN LL+ AV  LN  TK S+    LD++
Sbjct: 121 VNTLQPVGCLPQLTIQSSYQQCDQTQNSLASFHNQLLQVAVTTLNNNTKKST-FLPLDLF 180

Query: 276 GAFMSIIEGEKRYNNGV-FKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWD 335
            +F ++++ +      + F TPLKPCC    +  +CGSVD +GK ++ +C +P+  F+WD
Sbjct: 181 TSFNTVLKNKGDITGNLKFDTPLKPCCMATTNTSNCGSVDEKGKPLYTVCNEPESMFFWD 240

Query: 336 AVHPTQQGWAAAFGFCAYFIYFTISVSSLSNFFHLGRGSAGXXXXXXXXXXXXLRHRFRP 395
            VHPTQ GW A F                                               
Sbjct: 241 TVHPTQAGWRAVF----------------------------------------------- 300

Query: 396 TKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLKSPI 455
                                                                +G+KSP+
Sbjct: 301 -----------------------------------------------------MGIKSPL 360

Query: 456 PYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNTDAQ 515
           PY +      +   + G+NFAFGGTGVFDT    PN+TTQ  + Q L   + V+T  D +
Sbjct: 361 PYRVTKYAPKK--LQAGINFAFGGTGVFDTGNFQPNLTTQTGYLQGLIK-NKVYTKRDLE 420

Query: 516 SSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGLGPL 575
           SS+A+V+ SGNDYS + A   ++Q L  FI  VVNQI ++LKRI  LG+ ++ V  L P+
Sbjct: 421 SSLAMVTVSGNDYSAFTAAGGTQQNLPAFITRVVNQISIDLKRIHDLGIPRVLVNTLQPV 480

Query: 576 GCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFMSII 635
           GCLP  T  SSY++C++T NSL  FHN LL+ AV  LN  TK S+    +D++ +F +++
Sbjct: 481 GCLPQLTIQSSYQQCDQTQNSLASFHNQLLQVAVTTLNNNTKKST-FLPLDLFTSFNTVL 504

Query: 636 EGRKRYNNGV-FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQ 695
           + +      + F TPLKPCC    +  +CGSVD  GK ++  C + ++ FFWD+VHPTQ 
Sbjct: 541 KNKGDITGNLKFDTPLKPCCMATTNTSNCGSVDENGKPLYTVCNEPESMFFWDTVHPTQA 504

Query: 696 GWAAAFSAFKSF 706
           GW A   +  +F
Sbjct: 601 GWRAVSQSLGTF 504

BLAST of Carg10471 vs. TrEMBL
Match: tr|A0A1S3CH67|A0A1S3CH67_CUCME (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500887 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 1.3e-104
Identity = 203/346 (58.67%), Postives = 242/346 (69.94%), Query Frame = 0

Query: 366 FFHLGRGSAGXXXXXXXXXXXXLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGIT 425
           F H G+GS+                 F P KLFVFGDSY DTGNI    S A  FPYGIT
Sbjct: 17  FLHFGQGSSAV--------------HFHPLKLFVFGDSYVDTGNIHVNASSARNFPYGIT 76

Query: 426 FPGKPIGRFSDGRVLTDFAARHVGL-KSPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDT 485
           FPG P GRFSDGRVLTDF A++VGL KSPIP+++  R G R+  + G+NF FGGTGVFDT
Sbjct: 77  FPGVPSGRFSDGRVLTDFLAKYVGLKKSPIPFTVWQRFG-RKGAKYGMNFGFGGTGVFDT 136

Query: 486 EYPLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFI 545
            +P PNMTTQI+FF+ L   +S+F++ D  SS+ALVS SGNDYSFYF  N S +G KPFI
Sbjct: 137 SFPFPNMTTQINFFRNL-IVNSIFSSYDIHSSIALVSPSGNDYSFYFTNNGSLEGFKPFI 196

Query: 546 MSVVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLL 605
            SVVNQI VNLKRI  LGVKK+ V GLGP+GC P  TAP S+KKCN+T++S+  FHN LL
Sbjct: 197 FSVVNQISVNLKRIYKLGVKKVVVLGLGPVGCYPKPTAP-SFKKCNKTMDSIAVFHNTLL 256

Query: 606 KEAVKKLNQETKHSS-KVFIVDVYGAFMSIIEGRKRYNNG-VFKTPLKPCCHGVGSGFSC 665
           K+AV+KLN ETK  S   FI+++Y   +SII+ ++    G  FKTPLKPCC GV S FSC
Sbjct: 257 KQAVEKLNYETKEGSPNFFILNMYDTVLSIIKNKENRKGGATFKTPLKPCCFGVNSNFSC 316

Query: 666 GSVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKSFRNH 709
           GSVD  G KM+  C+    A FWD+VHPTQ GW A FS+FKS   H
Sbjct: 317 GSVDERGNKMYTLCKRPDLALFWDAVHPTQNGWFATFSSFKSTLKH 345

BLAST of Carg10471 vs. TrEMBL
Match: tr|A0A0A0KIM6|A0A0A0KIM6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452040 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 3.8e-104
Identity = 203/341 (59.53%), Postives = 240/341 (70.38%), Query Frame = 0

Query: 368 HLGRGSAGXXXXXXXXXXXXLRHRFRPTKLFVFGDSYADTGNILFPISPALQFPYGITFP 427
           HLG+GS+             +   F P KLFVFGDSY DTGNI    S A  FPYGITFP
Sbjct: 19  HLGQGSSS-----------PVHAHFYPLKLFVFGDSYVDTGNINVNTSSARNFPYGITFP 78

Query: 428 GKPIGRFSDGRVLTDFAARHVGLK-SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEY 487
           G P GRFSDGRVLTDF A +VGLK SPIP++   + G +   + G+NFAFGGTGVFDT Y
Sbjct: 79  GFPSGRFSDGRVLTDFLANYVGLKRSPIPFTAWKKTGSKLRAKCGINFAFGGTGVFDTLY 138

Query: 488 PLPNMTTQIDFFQQLRHGDSVFTNTDAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMS 547
           P PNMTTQI+FFQ L   +S+FT+ D  SS+ALVS SGNDYSFY A N S +G+KPF++S
Sbjct: 139 PFPNMTTQINFFQNL-IANSIFTSYDIHSSIALVSPSGNDYSFYLATNGSPEGVKPFVIS 198

Query: 548 VVNQIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKE 607
           VVNQI VNLKRI  LGVKKI V GLGP+GC P  TAP S+KKCNET+NSL  FHN LLK+
Sbjct: 199 VVNQISVNLKRIYKLGVKKIVVVGLGPVGCYPSPTAP-SFKKCNETMNSLAVFHNTLLKQ 258

Query: 608 AVKKLNQETK--HSSKVFIVDVYGAFMSIIEGRKRYNNG-VFKTPLKPCCHGVGSGFSCG 667
           AV+KLN ETK   S   FI+++Y   +SII+ +     G  F+TPLKPCC GV S FSCG
Sbjct: 259 AVEKLNSETKLGGSPNFFILNMYDTVLSIIKNKGNPKVGATFQTPLKPCCFGVSSNFSCG 318

Query: 668 SVDGEGKKMFVFCEDQKAAFFWDSVHPTQQGWAAAFSAFKS 705
           SVD  G KM+  C+    A FWD+VHPTQ+GW A F++  S
Sbjct: 319 SVDEHGNKMYTLCKRPDLALFWDTVHPTQKGWFATFNSLIS 346

BLAST of Carg10471 vs. TrEMBL
Match: tr|A0A2P6RBN6|A0A2P6RBN6_ROSCH (Putative acetylajmaline esterase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0472731 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 4.3e-100
Identity = 182/313 (58.15%), Postives = 233/313 (74.44%), Query Frame = 0

Query: 392 FRPTKLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDFAARHVGLK 451
           +RPTKLFVFGDSY+DTGN +  ++ + ++PYGITFPGKP GRFSDGRVLTDF A+ VG+K
Sbjct: 50  WRPTKLFVFGDSYSDTGNNMKSVTKSWKYPYGITFPGKPTGRFSDGRVLTDFLAKFVGVK 109

Query: 452 SPIPYSLRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMTTQIDFFQQLRHGDSVFTNT 511
           SPIPY  RN VG++  K  G+NFA+GGTGVF+T    PNMTTQIDFFQQ    DSVF   
Sbjct: 110 SPIPYKFRN-VGVKHLK-YGMNFAYGGTGVFNTLVAAPNMTTQIDFFQQFIK-DSVFNAQ 169

Query: 512 DAQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQIIVNLKRIGGLGVKKIAVTGL 571
           D  SSVAL++ +GNDY++Y A+N+S +G KPFI SVVNQ+ VNLKR+  LGVKKI VT L
Sbjct: 170 DLHSSVALITLAGNDYAYYLALNNSSEGFKPFITSVVNQLSVNLKRVYDLGVKKIVVTAL 229

Query: 572 GPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAVKKLNQETKHSSKVFIVDVYGAFM 631
            PLGCLP  TA  S++KCN T N LV FHN LL++A+ KLN E+K  S   ++D+Y +FM
Sbjct: 230 QPLGCLPGSTAELSFQKCNGTQNLLVGFHNLLLQQAIAKLNNESK--SSFIVLDLYTSFM 289

Query: 632 SIIEGRKRYNNGV-FKTPLKPCCHGVGSGFSCGSVDGEGKKMFVFCEDQKAAFFWDSVHP 691
           S+++ +  +   + F+ PL+PCC G+ S + CGSVD  G K +  C++ K+AFFWD  HP
Sbjct: 290 SVLKNKGDHLGSIKFENPLRPCCVGISSEYDCGSVDDSGAKKYTICDNPKSAFFWDGAHP 349

Query: 692 TQQGWAAAFSAFK 704
           TQQGW A +SA +
Sbjct: 350 TQQGWLAVYSALQ 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023543956.12.1e-19193.94GDSL esterase/lipase At5g03610-like, partial [Cucurbita pepo subsp. pepo][more]
XP_022925652.11.6e-18698.27GDSL esterase/lipase At5g03610-like isoform X3 [Cucurbita moschata][more]
XP_022925650.14.0e-18296.27GDSL esterase/lipase At5g03610-like isoform X1 [Cucurbita moschata][more]
XP_022925651.14.0e-18296.27GDSL esterase/lipase At5g03610-like isoform X2 [Cucurbita moschata][more]
XP_023544846.17.6e-18195.34GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G03610.13.7e-9755.41GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT3G09930.13.1e-8850.80GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT5G03600.13.3e-6945.17SGNH hydrolase-type esterase superfamily protein[more]
AT2G36325.17.5e-6643.91GDSL-like Lipase/Acylhydrolase superfamily protein[more]
AT5G03590.11.5e-3433.22GDSL-like Lipase/Acylhydrolase superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9LZS7|GDL71_ARATH6.7e-9655.41GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2... [more]
sp|Q9SF94|GDL50_ARATH5.7e-8750.80GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2... [more]
sp|Q9LZS8|GDL70_ARATH5.9e-6845.17GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3... [more]
sp|Q9LZS9|GDL69_ARATH2.5e-6645.98GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3... [more]
sp|Q8RWJ4|GDL45_ARATH1.4e-6443.91GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CJ29|A0A1S3CJ29_CUCME1.9e-12765.34GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=4... [more]
tr|A0A2U1N218|A0A2U1N218_ARTAN3.9e-10937.09SGNH hydrolase-type esterase domain-containing protein OS=Artemisia annua OX=356... [more]
tr|A0A1S3CH67|A0A1S3CH67_CUCME1.3e-10458.67GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500887 PE=4... [more]
tr|A0A0A0KIM6|A0A0A0KIM6_CUCSA3.8e-10459.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452040 PE=4 SV=1[more]
tr|A0A2P6RBN6|A0A2P6RBN6_ROSCH4.3e-10058.15Putative acetylajmaline esterase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g047... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: INTERPRO
TermDefinition
IPR035669SGNH_plant_lipase_like
IPR036514SGNH_hydro_sf
IPR001087GDSL
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016788 hydrolase activity, acting on ester bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10471-RACarg10471-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 45..344
e-value: 7.6E-13
score: 48.8
coord: 397..702
e-value: 1.2E-11
score: 44.9
IPR036514SGNH hydrolase superfamilyGENE3DG3DSA:3.40.50.1110coord: 28..347
e-value: 2.4E-31
score: 111.4
coord: 380..704
e-value: 3.9E-31
score: 110.7
NoneNo IPR availablePANTHERPTHR22835:SF324SUBFAMILY NOT NAMEDcoord: 388..704
coord: 34..347
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 388..704
coord: 34..347
NoneNo IPR availableSUPERFAMILYSSF52266SGNH hydrolasecoord: 326..347
coord: 42..61
coord: 121..285
NoneNo IPR availableSUPERFAMILYSSF52266SGNH hydrolasecoord: 678..702
coord: 394..413
coord: 451..637
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 397..702
e-value: 2.08929E-83
score: 267.944

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg10471Silver-seed gourdcarcarB172
Carg10471Silver-seed gourdcarcarB276
Carg10471Silver-seed gourdcarcarB280
Carg10471Silver-seed gourdcarcarB281
Carg10471Cucumber (Gy14) v2carcgybB0254
Carg10471Cucumber (Gy14) v2carcgybB0255
Carg10471Cucumber (Gy14) v1carcgyB0279
Carg10471Cucumber (Gy14) v1carcgyB0282
Carg10471Cucurbita maxima (Rimu)carcmaB0333
Carg10471Cucurbita maxima (Rimu)carcmaB0334
Carg10471Cucurbita maxima (Rimu)carcmaB0331
Carg10471Cucurbita maxima (Rimu)carcmaB0332
Carg10471Cucurbita maxima (Rimu)carcmaB0335
Carg10471Cucurbita moschata (Rifu)carcmoB0334
Carg10471Cucurbita moschata (Rifu)carcmoB0335
Carg10471Cucurbita moschata (Rifu)carcmoB0336
Carg10471Cucurbita moschata (Rifu)carcmoB0337
Carg10471Cucurbita moschata (Rifu)carcmoB0338
Carg10471Cucurbita pepo (Zucchini)carcpeB0337
Carg10471Cucurbita pepo (Zucchini)carcpeB0338
Carg10471Cucurbita pepo (Zucchini)carcpeB0339
Carg10471Cucurbita pepo (Zucchini)carcpeB0340
Carg10471Wild cucumber (PI 183967)carcpiB0269
Carg10471Wild cucumber (PI 183967)carcpiB0271
Carg10471Cucumber (Chinese Long) v2carcuB0265
Carg10471Cucumber (Chinese Long) v2carcuB0267
Carg10471Cucumber (Chinese Long) v3carcucB0269
Carg10471Cucumber (Chinese Long) v3carcucB0270
Carg10471Bottle gourd (USVL1VR-Ls)carlsiB212
Carg10471Bottle gourd (USVL1VR-Ls)carlsiB214
Carg10471Bottle gourd (USVL1VR-Ls)carlsiB215
Carg10471Melon (DHL92) v3.5.1carmeB0241
Carg10471Melon (DHL92) v3.5.1carmeB0242
Carg10471Melon (DHL92) v3.6.1carmedB0249
Carg10471Melon (DHL92) v3.6.1carmedB0250
Carg10471Watermelon (Charleston Gray)carwcgB0229
Carg10471Watermelon (Charleston Gray)carwcgB0231
Carg10471Watermelon (Charleston Gray)carwcgB0232
Carg10471Watermelon (97103) v1carwmB0225
Carg10471Watermelon (97103) v1carwmB0227
Carg10471Watermelon (97103) v1carwmB0228
Carg10471Watermelon (97103) v2carwmbB0240
Carg10471Watermelon (97103) v2carwmbB0242
Carg10471Watermelon (97103) v2carwmbB0244
Carg10471Wax gourdcarwgoB0302